Digital Commons Network^™

Model Validation And Transition To Turbulence In Orbiting Culture Dishes., Jonathan Michael D. Thomas

Electronic Theses and Dissertations

Wall shear stress (WSS) is commonly accepted as the primary influence affecting characteristics of anchored endothelial cells when subjected to fluid flow. Orbital shakers are commonly used to study cellular responses due to their ease of use, ability to run several experiments simultaneously, and since they exert physiologically relevant oscillatory shear. These studies require comprehensive resolution of WSS, however the fluid dynamics inside orbiting culture dishes has not yet been well described since the flow is complex and difficult to quantify analytically. A computational fluid dynamics (CFD) model of flow in an orbiting dish has been developed that yields detailed …

Identification Of Predictors And Moderators Of Weight-Related Behaviors In College Students., Heather Owens

Electronic Theses and Dissertations

College students are at increased risk for weight-gain and obesity compared to their non-student peers. Although many studies have confirmed these findings, few address the underlying causes of weight-related behaviors in college students. Associations between sleep behaviors (sleep duration and sleep quality), stress and weight-related behaviors (physical activity and food choices) have been identified, but studies have not adequately explored these associations among college students. Further, the influence of bioecological determinants of health cannot be overlooked when addressing health-related behaviors in diverse populations. The purpose of this dissertation was to explore factors impacting sleep behaviors and weight-related behaviors in college …

Microbiota Activated Cd103+ Dcs Stemming From Oral Microbiota Adaptation Specifically Drive Γδt17 Proliferation And Activation., Christopher Jay Fleming

Electronic Theses and Dissertations

IL-17 producing γδ T cells (γδT17) promote numerous autoimmune diseases such as psoriasis and arthritis, as well as, cancers of the colon, lung and breasts. Yet γδT17 peripheral regulation has yet to be thoroughly explored. In mice deficient in IL-17 signaling, we observed expansion of γδT17 in all major tissues. However, γδT17 expansion was not uniformly distributed systemically and was most prominent in oral draining cervical lymph nodes (LNs) with monoclonal expansion of Vγ6 γδT17. In vitro proliferation assays of these cervical LNs showed endogenous proliferation by γδT17 dependent on cell-to-cell contact with CD103⁺ DCs. CD86⁺ and CD80 …

Integrated Analysis Of Mirna/Mrna Expression And Gene Methylation Using Sparse Canonical Correlation Analysis., Dake Yang

Electronic Theses and Dissertations

MicroRNAs (miRNAs) are a large number of small endogenous non-coding RNA molecules (18-25 nucleotides in length) which regulate expression of genes post-transcriptionally. While a variety of algorithms exist for determining the targets of miRNAs, they are generally based on sequence information and frequently produce lists consisting of thousands of genes. Canonical correlation analysis (CCA) is a multivariate statistical method that can be used to find linear relationships between two data sets, and here we apply CCA to find the linear combination of differentially expressed miRNAs and their corresponding target genes having maximal negative correlation. Due to the high dimensionality, sparse …

An Environmental History Of The Rio Grande In The Panama Canal Zone, 1521- 1950., Francisco Javier Bonilla

Electronic Theses and Dissertations

This thesis is an examination of the history of environmental change in the defunct Rio Grande river valley in the former Panama Canal Zone. By mining secondary sources and engineering records, this study provides a narrative of the historic river’s role in the formation of the so-called interoceanic corridor through the isthmus of Panama from 1521 to 1950. More importantly, however, as a case study of river histories in Latin American environmental history, this understudied, Pacific-draining river illustrates how nature and non-human nature made Panama’s strategic transit region into a loci for material flows over time. The Rio Grande played …

Sparse Feature Learning For Image Analysis In Segmentation, Classification, And Disease Diagnosis., Ehsan Hosseini-Asl

Electronic Theses and Dissertations

The success of machine learning algorithms generally depends on intermediate data representation, called features that disentangle the hidden factors of variation in data. Moreover, machine learning models are required to be generalized, in order to reduce the specificity or bias toward the training dataset. Unsupervised feature learning is useful in taking advantage of large amount of unlabeled data, which is available to capture these variations. However, learned features are required to capture variational patterns in data space. In this dissertation, unsupervised feature learning with sparsity is investigated for sparse and local feature extraction with application to lung segmentation, interpretable deep …

Porphyromonas Gingivalis Gingipains Induce A Pro-Inflammatory Extracellular Microenvironment : The Role Of Par-2 And Fibronectin., Jeffrey S. Marschall

Electronic Theses and Dissertations

Periodontitis is a chronic inflammatory disease that is characterized by severe tissue destruction of the gingiva and other tooth supporting structures; if left untreated, tooth loss and disintegration of the alveolar bone occurs. This chronic inflammatory state has been linked to other systemic diseases such as cardiovascular disease, diabetes, rheumatoid arthritis, and Alzheimer’s disease. Porphyromonas gingivalis is the major pathogenic microbe in periodontitis. The main virulence factors of P. gingivalis are the Arg-aa and Lys-aa gingipains, which are proteolytic enzymes implicated in a plethora of activities that allow P. gingivalis to subvert the human immune system in the oral cavity …

Inference For A Zero-Inflated Conway-Maxwell-Poisson Regression For Clustered Count Data., Hyoyoung Choo-Wosoba

Electronic Theses and Dissertations

This dissertation is directed toward developing a statistical methodology with applications of the Conway-Maxwell-Poisson (CMP) distribution (Conway, R. W., and Maxwell, W. L., 1962) to count data. The count data for this dissertation exhibit three different characteristics: clustering, zero inflation, and dispersion. Clustering suggests that observations within clusters are correlated, and the zero inflation phenomenon occurs when the data exhibit excessive zero counts. Dispersion implies that the mean is greater/smaller than the variance unlike a Poisson distribution. The dissertation starts with an introduction of inference for a zero-inflated clustered count data in the first chapter. Then, it presents novel methodologies …

Adaptive Schools : Investigating Impact, Continuity, And Change In One School District., Winn Crenshaw Wheeler

Electronic Theses and Dissertations

This dissertation is an examination of the work of Adaptive Schools in Smith County Schools. It begins by explaining how the work of Adaptive Schools is relevant in the larger context of school reform. To explore this relevance, a qualitative analysis of Smith County was conducted. Smith County was selected based on systematic implementation of the work of Adaptive Schools. Analysis of interview data focused on: the impact of the work of Adaptive Schools within Smith County and the degree to which Adaptive Schools intersected with themes of continuity and change. Further analysis explores emergent questions about: (a) the function …

Abin1 In The Pathogenesis Of Glomerulonephritis And The Novel Polocyte-Neutrophil Proinflammatory Axis., Erik Anderson Korte

Electronic Theses and Dissertations

Glomerulonephritis (GN) is the most common cause of end-stage renal disease worldwide and is characterized by deposition of immune complex, inflammation, proteinuria and podocyte damage. Immunosuppressive therapy is effective in less than 50% of patients, indicating that immunological activity is not the only relevant cause of kidney injury. While methods have been described for neutrophil-mediated immune activation, specific glomerular mechanisms for recruitment of neutrophils and development of GN have not been determined. Here I present a pathogenic mechanism for the development of GN involving a novel podocyte-neutrophil axis and reveal the importance of glomerular NF-κB inhibition by the ubiquitin binding …

Insights Into The Introduction Histories And Population Genetic Dynamics Of The Nile Monitor (Varanus Niloticus) And Argentine Black And White Tegu (Salvator Merianae) In Florida., Jared Price Wood

Electronic Theses and Dissertations

This dissertation examines the population genetic dynamics of two Florida invasives: the Nile monitor (Varanus niloticus) and Argentine black and white tegu (Salvator merianae). I also provide insights into the introduction histories of both species. This study was developed as part of a collaborative effort with the Florida Wildlife Commission to expand our knowledge of these highly detrimental, invasive lizards. All research activities involving animals and animal tissues were approved by the University of Louisville’s Institutional Animal Care and Use Committee (IACUC Proposal #: 12024). I start with a brief introduction into what makes invasive species …

Modulation Of Cell Death Signaling And Cell Proliferation By The Interaction Of Homoserine Lactones And Paraoxonase 2., Aaron Mackallan Neely

Electronic Theses and Dissertations

Pseudomonas aeruginosa produces N-(3-oxododecanoyl)-homoserine lactone (C12) as a quorum-sensing molecule that functions to facilitate bacteria-bacteria communication. C12 has also been reported to affect many aspects of human host cell physiology, including evoking cell death in various types of cells. However, the signaling pathway(s) leading to C12-triggerred cell death remains unclear. To clarify cell death signaling induced by C12, we examined mouse embryonic fibroblasts (MEFs) deficient in one or more caspases. Our data indicate that, unlike most apoptotic inducers, C12 evokes a novel form of apoptosis in cells, probably through the direct induction of mitochondrial membrane permeabilization. Previous studies indicate that …

Exploring Distributed Instructional Leadership : Case Studies Of Assistant Principals In An Urban School District., Jimica Claudette Howard

Electronic Theses and Dissertations

This study examined the role of instructional assistant principals in distributed instructional leadership in three middle schools in a large urban school district. Using the leadership functions in Hallinger’s (2011) framework of instructional leadership, the distribution of instructional leadership functions were examined. Interviews, document analysis, and an observation determined that the assistant principal in only one out of three cases was engaging in distributed instructional leadership. One was named an instructional assistant principal and functioned as an operations manager and the third was called a “lead” assistant principal and also spent most of his time on operational/management functions. Conclusions and …

Same-Sex Child Molestation Within The Boy Scouts Of America Initiation And Termination In Victim Narratives., Leah Catherine Jihee Shon

Electronic Theses and Dissertations

Previous studies of child molestation have largely used a sample of either incarcerated or convicted sex offenders. Previous studies have also failed to provide substantial analyses regarding how physical contacts between child victims and adult perpetrators were initiated and terminated at the crime scene; few studies have focused on “what exactly happened” while physical touching was taking place through an analysis of behavioral sequences of both child victims and adult offenders. Although previous literature on interpersonal child abuse used child victims of sexual abuse as their data source, those studies focused on effective victim treatment plans to deal with victims’ …

Acrolein Is A Critical Mediator Of Alcohol-Induced Liver And Intestinal Injury In Alcoholic Liver Disease., Wei-Yang (Jeremy) Chen

Electronic Theses and Dissertations

Alcohol consumption can cause alcoholic liver disease (ALD), which remains a major cause of morbidity and mortality in the United States. Chronic alcohol consumption causes a pro-oxidant environment in the liver and increases hepatic lipid peroxidation. Acrolein is the most reactive and toxic aldehyde generated through lipid peroxidation. Acrolein forms protein adducts and triggers endoplasmic reticulum (ER) stress and hepatocyte apoptosis, which are recognized etiologic factors in ALD. Several studies have established the critical role of the gut-liver axis in ALD pathogenesis, wherein alcohol-induced gut barrier dysfunction contributes to liver injury. This study investigates, in vitro and in vivo, the …

Woody Plant Communities Of Three Urban Wetlands And The Success Of An Invasive Shrub (Lonicera Maackii) Over Natural And Experimental Flooding Gradients., Meghan Rhea Langley

Electronic Theses and Dissertations

Globally, wetlands are known for providing important ecosystem services that enhance the quality of human life and regulating global biogeochemical cycles. Despite the wide recognition of their value, temperate forested wetlands are the least protected type of ecosystem world-wide, and are threatened by human activities such as logging and development. The ecology of forested wetlands remaining in urbanized areas is impacted by a multitude of anthropogenic threats, including fragmentation (which decreases the amount of interior habitat and increases edge habitat), hydrologic modification (ditching and draining of wetlands) and the incursion of invasive species (which are frequently introduced by human activities). …

Chemoselective Reactions And Their Applications In Metabolite Isolation And Analysis., Sadakatali Shokatali Gori

Electronic Theses and Dissertations

Chemoselectivity is the preferential reaction of a reagent with a select functional group among other plausible reactions. This dissertation describes chemoselective reagents and methods explored to develop more accurate, efficient and high-throughput means to quantify metabolites containing either thiol, carboxylic acid or 1,2-diol moieties directly from biological mixtures. Chapter 1 discusses how dysregulation of thiol- or carboxylic acid-containing metabolites is either the cause or effect of cellular dysfunction. Quantifying such metabolites is important in discovery of pathways to understand etiology of diseases. Chapter 1 also discusses the need for sustainable syntheses using renewable resources and describes the traditional methods for …

Relationship Of Force Variables To Vertical Jumps Performance, Hugo A. De Paula Santana

Electronic Theses and Dissertations

The isometric mid-thigh pull (IMTP) has been cited often in the scientific literature; however, there is still a lack of agreement as to the ideal body position used during this test, and how body position impacts the relationship between IMTP performance and dynamic performance. Thus, one aim of this dissertation was to compare two different IMTP positions and correlate the kinetic outputs from each position to vertical jump (VJ) performance. Another purpose of this dissertation was analyze which method of data normalization for IMTP force variables best correlates to squat jump (SJ) and countermovement jump (CMJ) performance.

In the first …

Microrna-186 And Metastatic Prostate Cancer., Dominique Zilpha Jones

Electronic Theses and Dissertations

MicroRNA (miR) dysregulation alters cancer-associated gene expression, which contributes to cancer pathogenesis. For example, miR-186 over expression lead to enhanced proliferation and migration in pancreatic cancer cell models. However, the role of miR-186 in prostate cancer (PCa) remains controversial. Previously, miR-186-5p was up-regulated in PCa patient serum (stage III/IV) compared to controls. Furthermore, miR-186-5p was up-regulated in metastatic PCa (PC-3, MDA PCa 2b, LNCaP) relative to normal prostate epithelial cells (RWPE1). We hypothesized miR-186 inhibition will reduce aggressive PCa using metastatic cell models. To test this, we evaluated whether miR-186-5p inhibition would reduce aggressive PCa behavior and overexpression induce malignant …

Modulation Of Cardiac Kv Currents By Kvbeta2 And Pyridine Nucleotides., Peter Joseph Kilfoil

Electronic Theses and Dissertations

Myocardial voltage-gated potassium (Kv) channels regulate the resting membrane potential and the repolarization phase of the action potential. Members of the Kv1 and Kv4 family associate with ancillary subunits, such as the Kvβ proteins, that modify channel kinetics, gating and trafficking. Previous investigation into the function of cardiac β subunits demonstrated that Kvβ1 regulates I_to and I_K,slow currents in the heart, but the role of Kvβ2 in the myocardium remains unknown. In heterologous expression systems, Kvβ2 increases surface expression of Kv1 channels, shifts the activation potential of Kv1 channels to more polarized voltages, and increases the inactivation of …

The Queer Child And Haut Bourgeois Domesticity : Berthe Morisot And Mary Cassatt., Jessica Cresseveur

Electronic Theses and Dissertations

Since the 1970s, feminist art historians have extensively treated Mary Cassatt and Berthe Morisot. In particular, focusing on class-bound womanhood and domesticity, Griselda Pollock, Linda Nochlin, and Anne Higonnet have provided compelling psychoanalytic, Marxist, and semiotic analyses, seemingly exhausting all potentials for any further historical exploration of these artists. Yet, to date, investigations into the significance of the queer (deviations from normative sociocultural codes of gender identity, sexuality, and reproduction) in the works of Cassatt and Morisot have not been conducted. In this dissertation, queer theory complements the existing scholarship that has focused on the significance of women as mothers …

Predicting Nclex-Rn Performance : An Exploration Of Student Demographics, Pre-Program Factors, And Nursing Program Factors., Heather Davis Mitchell

Electronic Theses and Dissertations

Nursing programs are experiencing a decline in National Council Licensure Examination for Registered Nurses (NCLEX-RN) pass rates among graduates. While researchers have attempted to identify predictors of performance on the NCLEX-RN, identification of predictors remains elusive. Although the literature is replete with studies exploring NCLEX-RN predictors, prediction under the new 2013 NCLEX test plan and passing standards is not well established. Considering the ever-evolving diversity in students, combined with recent changes in the NCLEX-RN, further exploration of predictors of performance is warranted. Using a correlational design, the study sought to identify the predictors of NCLEX-RN performance for Bachelors of Science …

Long Term Facial Alveolar Bone Changes Associated With Endosseous Implants In The Anterior Maxilla., Deepika Joshi

Electronic Theses and Dissertations

Aim: To quantify the radiographic presence and thickness of facial alveolar bone (FAB_r) adjacent to implants placed in the anterior maxillary region. Lack of FAB_r may suggest that graft procedures are desirable prior to implant placement. Material and Methods: With IRB approval, a retrospective analysis of cone beam computed tomographic cross-sectional images of sites with at least one implant in the anterior maxillary region was performed. Details regarding type of implant and location were recorded. FAB_r perpendicular to the long axis each implant was measured at seven levels by two observers independently and means and standard …

Identities Without Origins : Fat/Trans Subjectivity And The Possibilities Of Plurality., Mc Lampe

Electronic Theses and Dissertations

This project draws upon the work of Michel Foucault, Judith Butler, and Donna Haraway to critically analyze the political power and utility of origin stories as they are used within discourses of identity. I specifically examine the dominant cultural and counter-origin stories of transgender and fat bodies and argue that the counter origin stories constructed by both trans and fat studies/activism continue to engage with norms that regulate identity. These regulations create an impossible situation for individuals who are both trans and fat as they are not recognized as intelligible subjects within either category due to their lack of appropriate …

Role Of Microrna-21 In Atherogenesis., Rihab Hamed-Berair

Electronic Theses and Dissertations

MicroRNA-21 (miR-21) is an evolutionarily conserved microRNA, abundant in most cardiovascular tissues. It has been implicated in the pathogenesis of several cardiovascular diseases including restenosis, myocardial infarction, and heart failure. However, little is known about the contribution of miR-21 in atherosclerosis. My data show that expression of miR-21 is increased by >1.5-fold in murine atherosclerotic lesions and by 1.5-2.0-fold in the macrophages of Western diet (WD)-fed LDLR-KO mice (for 12-20 weeks). In vitro, LDL, oxidized LDL, acetylated LDL and LPS induced miR-21 by 2-4-fold and down-regulated its target protein, PDCD4, in bone marrow-derived macrophages. Basally, macrophages isolated from miR-21-KO …

Influence Of In-Office Oral Hygiene Instructions On The Parental Assessment Of Their Child's Oral Health., Sireesha Kodali

Electronic Theses and Dissertations

Purpose: To identify the relation between oral health education and parental assessment of their child’s dental condition. Methods: Our study population included 33,342 children, from Smile KY! annual screenings from 2002 to 20014. Data collection was through questionnaire forms. Our primary risk factor was “dental health instructions received” (yes/no) and our outcome of interest was “parental assessment of your child’s dental health” (good/fair/poor). We had 13 years of data from 2002 to 2014 and used ‘SAS’ statistical software for data manipulation and recoding. Descriptive statistics, chi-square test of independence, trend analysis and multiple logistic regression modeling were performed on ‘SPSS’ …

The Agricultural Growth And Malting Production Of Barley Grains In Northeast Tennessee And Southwest Virginia, Mark Evans Patton Mr.

Electronic Theses and Dissertations

The purpose of this study is to explore the history and production processes of beer and examines the components that contribute to its brewing. The narrative will provide a timeline of the processes that are required to create the product of beer beginning with the agricultural products and following the brewing processes that result in beer as the finished product. I will also examine the business of agriculture and grain processing and will provide some historical perspectives of grain, beer, and malting. I will also consider whether the growing, processing, and malting of grains in the East Tennessee and Southwest …

Ecological Niche Modeling Of The North American Giant Salamander: Predicting Current And Future Potential Distributions And Examining Environmental Influences, Selena S. Roark

Electronic Theses and Dissertations

North American Giant Salamanders (Cryptobranchus alleganiensis), commonly known as hellbenders, have been experiencing a population decline for decades due to human influences, such as pollution and habitat destruction. Many efforts are underway to save the hellbender but their entire potential geographical range has not been well-studied. Currently, hellbender populations are delineated by county boundaries and are on the International Union for Conservation of Nature Red List. The Genetic Algorithm for Rule-set Production, an Ecological Niche Model, was used to model the current hellbender potential distribution at a macro-scale under two different environmental scenarios. Additionally, future potential distributions were …

Automated Analysis Of Cancerous Histological Samples., Thomas Allen Tennill

Electronic Theses and Dissertations

The use of immunohistochemistry has become commonplace in the field of cancer diagnosis. A major limitation to the ability to use this tool effectively is the time consuming tasks required for the analysis of data due to the fact that it is largely done by hand. Additionally, because of this, there is an inherent level of subjectivity in the results obtained from this process that may depend on who it is conducting the analysis. Therefore, there exists a need for a method that is able to quantify results from immunohistochemical techniques in a way that it is both time-effective and …

Development Of A Novel Device For Ventricular Assist Device Outflow Graft Anastomosis., Young Choi

Electronic Theses and Dissertations

Purpose: Left ventricular assist device (LVAD) therapy can be live-saving for advanced heart failure patients. Conventional anastomosis (surgical connection) of LVAD outflow grafts to the aorta requires aortic clamping and hand-suturing. Aortic clamping increases the risk for neurological complications. Hand-suturing may be time-consuming and requires significant surgical dexterity. There is currently no commercially available device for sutureless anastomosis of large vascular grafts (diameter > 5mm). To overcome these limitations, a prototype LVAD outflow graft anastomosis device (GrAD) that facilitates sutureless anastomosis was developed and tested to demonstrate proof-of-concept and feasibility for (1) secured attachment withstanding physiological pressures, and (2) comparable attachment …