Digital Commons Network^™

Inhibition Of Chemotherapeutic-Induced Apoptosis In Esophageal Squamous Cancer Cells By The Keystone Periodontal Pathogen, Porphyromonas Gingivalis., Atul Kumar Agrawal

Electronic Theses and Dissertations

Recent evidence has shown that P. gingivalis, a Gram-negative, anaerobic oral bacterium, is negatively associated with the presence and outcome of esophageal squamous cell carcinoma (ESCC). While potential underlying mechanisms are yet to be established, P. gingivalis infection is known to inhibit apoptosis in gingival epithelial cells. Therefore, we hypothesized that P. gingivalis may also inhibit of the induction of apoptosis in human ESCC cells exposed to the commonly employed anti-ESCC chemotherapeutic agent, cisplatin. The capacity of P. gingivalis, at variant multiplicities of infection, to suppress cisplatin-induced necrosis and apoptosis in EC9706 ESCC cells was established by lactate …

Identifying The Signaling Mechanisms Of Egfr-Mediated Apoptosis., Nicole Marion Jackson

Electronic Theses and Dissertations

The Epidermal Growth Factor Receptor (EGFR) is a 170-kilodalton transmembrane protein that belongs to the ErbB family of receptor tyrosine kinases. Upon ligand-mediated activation, the EGFR is responsible for cell growth, proliferation, and tissue homeostasis; however, the EGFR is overexpressed in many human malignancies, including MDA-MB-468 cells, a metastatic breast epithelial cell line. Studies within this cell line, and other cell lines characterized with high EGFR levels, have shown that EGF stimulation results in the induction of apoptosis. However, the mechanisms and signaling effectors implicated in this process have yet to be elucidated. The overarching research goal of this dissertation …

Assessment Of Social, Dietary And Biochemical Correlates Of Cardiometabolic Risk In Pre-Adolescent Hispanic Children, Abraham Basil Alhassan

Electronic Theses and Dissertations

Obesity, elevated blood pressure and dyslipidemia are highly prevalent in Hispanic children. Compared to their non-Hispanic White peers, Hispanic children experience higher prevalence of obesity and hypertension. The Hispanic population in Tennessee has been growing, with about a tenth of newborn babies being Hispanic. This study aimed to: 1. Examine the influence of sociodemographic factors on Hispanic children’s cardiometabolic risk; 2. Assess the relationship between food group intake and cardiometabolic risk in Hispanic children; and 3. Evaluate the efficacy of non-traditional biomarkers for detecting cardiometabolic risk in Hispanic children. Data for the study came from a larger cross-sectional pilot study …

A Novel Mra-Based Framework For The Detection Of Changes In Cerebrovascular Blood Pressure., Yitzhak Atakilt Gebru

Electronic Theses and Dissertations

Background: High blood pressure (HBP) affects 75 million adults and is the primary or contributing cause of mortality in 410,000 adults each year in the United States. Chronic HBP leads to cerebrovascular changes and is a significant contributor for strokes, dementia, and cognitive impairment. Non-invasive measurement of changes in cerebral vasculature and blood pressure (BP) may enable physicians to optimally treat HBP patients. This manuscript describes a method to non-invasively quantify changes in cerebral vasculature and BP using Magnetic Resonance Angiography (MRA) imaging.

Methods: MRA images and BP measurements were obtained from patients (n=15, M=8, F=7, Age= 49.2 …

Examining The Effects Of Macrophage Populations On Cancerous Tumor Growth., Grace E. Mahlbacher

Electronic Theses and Dissertations

The most abundant immune cell types of the tumor microenvironment macrophages recruited there by tumor-eluted factors. The role of these immune cells in tumor progression, and the interplay between tumor and immune cells is an emerging field of research with potential for novel treatment strategies. Here, a TIE2 expressing macrophage (TEM) subtype is integrated into a virtual tumor model. Within the 2D microenvironment, the TEM will differentiate from an extravasated monocyte precursor, congregate around the abluminal side of the vasculature in response to a chemoattractant gradient, secrete cytokines which favor differentiation of a separate angiogenic macrophage subtype [1]. The effects …

Effect Of High Cationic Flocculant On Anaerobic Digestion Of Municipal Wastewater., Prathap D. John

Electronic Theses and Dissertations

Anaerobic digestion (AD) of municipal sludge is a widely used stabilization process at municipal wastewater treatment plants. It is highly effective in reducing sludge volume and produces methane gas which is used as fuel in the wastewater plant. Following digestion sludge is sent to a centrifuge to separate the solids from the liquids. Louisville MSD adds flocculent to material in the centrifuge to better coagulate the solids, thereby aiding in separation of the solids from the liquids. The potential for further AD, COD reduction, and methane generation of the liquid effluent from the centrifuge was unknown, particularly in regards to …

Video Game Engagement, Gender, And Age: Examining Similarities And Differences In Motivation Between Those Who May Or May Not Play Video Games, Joseph Camarata

Electronic Theses and Dissertations

This research aims to fill a research gap by examining video games to explore whether gender, age, or hours played per week would exert any influence on the information of those who may or may not play video games. Mood Management Theory and Uses and Gratification Theory were used as the theoretical foundation for this study. Four-hundred-three East Tennessee State University students who received the survey via email were asked to voluntarily participate in a survey about their motivations behind playing video games. Results from MANOVA showed that the motivations of male participants on video games were significantly higher than …

Trout Fishing In The Smokies And The Blue Ridge, 1880-Present: How-To, History, And Habitat, Nathaniel Cole Skaggs

Electronic Theses and Dissertations

This study focuses on trout fishing in the Great Smoky Mountains and the Blue Ridge Mountains from 1880 to 2017. I begin with a collection of personal narratives of fly-fishing in Tennessee to portray the allure of southern Appalachia trout fishing. I then describe the transition from native Cherokee fishing practices to sport fishing in the Smokies and the Blue Ridge by 1880. I explore a brief history of the National Parks and the United States Forest Service during the early 1900s, and address European fly-fishing influences in the United States during the twentieth century. I examine the habitats of …

The Establishment And Development Of The Mockingbird As The Nightingale’S “American Rival”, Gabe Cameron

Electronic Theses and Dissertations

During the eighteenth and nineteenth centuries, many British poets attempted to establish a universal poetic image in the European nightingale, often viewing it as a muse or contemporary artist. This use of the songster became so prevalent that it was adopted, along with other conventions, for use in the United States. Yet, despite the efforts of both British and American poets, this imperialized songbird would ultimately fail in America, as the nightingale is not indigenous to the United States. The failure of this nightingale image, I contend, is reflective of the growing need to establish a national identity in nineteenth-century …

Range-Wide Prevalence And Impacts Of Pseudocercosporella Inconspicua On Lilium Grayi And An Assessment Of L. Superbum And L. Michauxii As Reservoirs, Cindy L. Barrett

Electronic Theses and Dissertations

Lilium grayi (Gray’s Lily), a southern Appalachian endemic species, is threatened by a Lilium-specific fungal pathogen, Pseudocercosporella inconspicua. The disease is characterized by tan lesions that can cause early senescence, while also lowering seed production and viability. This project tested for P. inconspicua conidia and accessed health at nine locations. The disease was present and ubiquitous across the range of L. grayi. Through identification of P. inconspicua conidia in the field, L. superbum (Turk’s Cap Lily) was identified as an additional host, while L. michauxii (Michaux’s Lily) was disease-free. However, infection was inducible in both species. With …

Effects Of Predatory Cues On Copper Sensitivity And Metabolic Rate In Gambusia Affinis, Melissa N. Campbell

Electronic Theses and Dissertations

Organisms living in aquatic environments are subject to a number of stressors from natural (temperature, predation, food availability) and anthropogenic origins (pesticides, metals, etc). Natural stressors may amplify the effects of contaminants and increase an organism’s sensitivity to them. Understanding the impact of these combined factors is therefore essential for the practical management of contaminants. This study sought to examine how predatory cues affect copper tolerance in the mosquitofish, Gambusia affinis. The influence of predatory cues on fish metabolic rate was also evaluated to gain insight on any interactive effects between the natural stressor and copper. Alarm cues, chemicals released …

Student Self-Assessment Of Operative Dentistry Experiences : A Time Dependent Exercise In Best Practice Outcomes., Celine Joyce Cornelius Timothius

Electronic Theses and Dissertations

Purpose: The purpose of this project was to evaluate student self-assessment of operative dentistry experiences. Methods: First, a retrospective analysis of student self-assessment and faculty assessment grade sheets were evaluated for mean differences and correlations across time. Both preclinical (D2: n=120) and clinical (D3: n=120; D4: n=120) grades sheets were evaluated. Secondly, twenty-five (n=25) students from each of the D1, D2, D3 and D4 classes were asked to evaluate dentoform work. Twenty-five (n=25) operative calibrated faculty graded the same two dentoforms. Results: D2 student’s self-assessment scores were significantly higher than the faculty assessment scores (t-test; p0.05) and there was a …

Mindfulness And Alcohol-Related Problems Among Individuals With Fibromyalgia: Chronic Pain And Depressive Symptoms As Mediators, Julie I. Morrissey

Electronic Theses and Dissertations

Mindfulness is a cognitive attribute that is associated with better health and well-being. Fibromyalgia is a neurosensory disorder primarily characterized by chronic pain and comorbid depression, leading to an increased risk for alcohol-related problems. Empirical literature confirms mindfulness has beneficial associations with chronic pain, depression, alcohol-related problems, and fibromyalgia. Mindfulness may lead to better health and well-being by facilitating self-monitoring, objective reperceiving, and purposeful changing of health-related behaviors. It was hypothesized that higher levels of mindfulness would be related to lower levels of chronic pain and depressive symptoms, and, in turn, to fewer alcohol-related problems among individuals with fibromyalgia. Cross-sectional …

The Increasing Risk Of Vector-Borne Diseases: Mapping The Effects Of Climate Change And Human Population Density On Future Aedes Aegypti Habitats, Julie Obenauer

Electronic Theses and Dissertations

The Aedes aegypti mosquito is the vector for four infectious diseases of global concern – Yellow Fever, Dengue, Chikungunya, and Zikavirus. Previous attempts to model the expansion of the vector habitat due to global climate change have rarely included characteristics related to the human populations on which this mosquito is dependent. The purpose of this research was to determine whether the inclusion of human population density improves model performance while creating risk maps that can be used to determine where humans are most likely to be exposed to the vector in the future. The resulting model demonstrated that the inclusion …

Prevalence Of Communication Disorders In Children With Neonatal Abstinence Syndrome On School Speech-Language Pathology Caseloads: A National Survey, Brittany V. Ratliff

Electronic Theses and Dissertations

There is concern about the recently increasing number of infants born with Neonatal Abstinence Syndrome (NAS), yet little is known about its long-term neurodevelopmental effects. School-based speech-language pathologists (SLPs) are in a unique position to comment on potential long-term consequences of NAS because their caseloads include children with a variety of communication disorders and comorbidities. School-based SLPs across the United States (N = 258) responded to a survey about the presence of children with NAS on their caseloads and their perceptions of the children’s communication disorders and comorbidities. Results revealed that children with NAS currently are being treated by …

Kindergarten Teachers' Perceptions Of Student Readiness For School, James A. Wernke

Electronic Theses and Dissertations

The design method for this study was non-experimental quantitative. The survey was distributed via email to Kindergarten teachers in two Northeastern Tennessee school districts. There were 69 Kindergarten teachers who responded to the survey. The study revealed that Kindergarten teachers perceive that gender, socioeconomic status, and preschool experience have a significant impact on student readiness for school. Chronological age was not perceived to have an impact on student readiness for school to a significant extent. Kindergarten teachers perceived that preschool experience has the greatest impact on student readiness for school when asked to rank the order of impact from greatest …

Environmental Factors And School Disorder: The Role Of Urbanicty, Brandon S. Coffey

Electronic Theses and Dissertations

The purpose of this study is to explore the differential impact of various environmental and organizational factors on levels of school misconduct. Although we have a general understanding of this relationship, little effort has been made to determine whether the effects are influenced by urbanicity. The current study utilizes data from the 2007-2008 School Survey on Crime and Safety to address this gap in the literature by utilizing a series of negative binomial regression models that seek to determine differences between predictive factors in urban and rural settings. Results indicate that disorganization has a similar effect within urban and rural …

Peptide Identification: Refining A Bayesian Stochastic Model, Theophilus Barnabas Kobina Acquah

Electronic Theses and Dissertations

Notwithstanding the challenges associated with different methods of peptide identification, other methods have been explored over the years. The complexity, size and computational challenges of peptide-based data sets calls for more intrusion into this sphere. By relying on the prior information about the average relative abundances of bond cleavages and the prior probability of any specific amino acid sequence, we refine an already developed Bayesian approach in identifying peptides. The likelihood function is improved by adding additional ions to the model and its size is driven by two overall goodness of fit measures. In the face of the complexities associated …

Functionalized Silica Gel For Adsorption Of Cesium From Solution, Kenneth Marshall Seaton Iii

Electronic Theses and Dissertations

Mesoporous silica gel containing embedded phosphotungstic acid (PTA) was synthesized by sol-gel co-condensation of tetraethyl orthosilicate with PTA in acidic media. The obtained material had high Brunauer-Emmett-Teller Theory (BET) surface area and pore volume. A characteristic band of the Keggin structure of PTA was present in its FT-IR spectrum while its X-ray diffraction patterns were absent. This proved the embedding of PTA on a sub-molecular level and not as a second phase. Acidic sites were determined by neutralization with base in aprotic solvent, followed by titration of the remaining base with an acid. The material demonstrated high adsorption capacity of …

Friend Or Foe? Martial Race Ideology And The Experience Of Highland Scottish And Irish Regiments In Mid-Victorian Conflicts, 1853-1870, Adam Spivey

Electronic Theses and Dissertations

This thesis examines martial race ideology in the British Army during the mid-nineteenth century. A “martial race” was a group of people that the British considered to excel in the art of warfare due to biological and cultural characteristics. This thesis examines perceived “martial” natures or lack thereof of the Highland Scots and the Irish during this era. Central to this analysis are the Crimean War (1853-1856) and the Indian Mutiny of 1857 which provided opportunities for soldiers to display their “martial” qualities. The Crimean War was the first war where the daily newspapers covered every aspect of the war …

Denoising Tandem Mass Spectrometry Data, Felix Offei

Electronic Theses and Dissertations

Protein identification using tandem mass spectrometry (MS/MS) has proven to be an effective way to identify proteins in a biological sample. An observed spectrum is constructed from the data produced by the tandem mass spectrometer. A protein can be identified if the observed spectrum aligns with the theoretical spectrum. However, data generated by the tandem mass spectrometer are affected by errors thus making protein identification challenging in the field of proteomics. Some of these errors include wrong calibration of the instrument, instrument distortion and noise. In this thesis, we present a pre-processing method, which focuses on the removal of noisy …

Inclusive Teaching Strategies: An Evaluation Of Course Structure And Summative Assessment In Introductory Biology, Oluwaseun O. Agboola

Electronic Theses and Dissertations

Several active learning strategies have been used when increasing the structure of a course as increasing course structure has been known to improve student learning in introductory STEM courses. Much has been studied on the value of frequent formative assessment; however, few studies have evaluated the effective modes of delivering summative assessment. This study examines the use of summative assessment as an inclusive teaching practice to improve first generation college student success in introductory biology and also uses faculty surveys to find out how instructors structure their introductory biology course and why they are structured that way. Final exams were …

Spirituality And Suicidal Behavior: The Mediating Role Of Self-Forgiveness And Psychache, Benjamin B. Hall

Electronic Theses and Dissertations

Growing evidence for protective factors of spirituality against physical and mental health related outcomes has led to the consideration of spirituality as a protective factor for suicidal behaviors. Although initial support for this association is promising, spirituality has yet to be explored as it relates to psychache. Additionally, self-forgiveness has emerged as an important protective factor of suicidal behavior, but has not been explored in the context of psychache. Following a model developed by Webb, Hirsch, and Toussaint (2015), the current project explores the protective role of spirituality on suicidal behavior based on three dimensions of spirituality: ritualistic, theistic, and …

Self-Efficacy Sources And Academic Motivation: A Qualitative Study Of 10th Graders, Salina K. Bryant

Electronic Theses and Dissertations

The NAEP (2016) report shows that the performance of the country’s highest achievers is increasing in reading while the lowest-achieving students have lower scores than previous reports and are performing worse than ever. Not only are these students expected to succeed academically, these students must know how to problem solve, work in teams, and be creative. The longstanding issue of how to motivate students is not new. Motivation consists of the factors that stimulate the desire to attain a goal. Self-efficacy is defined as the belief in one's capabilities to carry out, organize and perform a task successfully (Bandura, 1997). …

Niche Modeling For The Giant Panda, Ailuropoda Melanoleuca, And The Original Panda, Ailurus Fulgens: Habitat Preferences And Evolutionary Consequences, Lauren M. Lyon

Electronic Theses and Dissertations

The well-known symbol for conservation, the giant panda bear, and the original red panda have been forced into remote habitats due to anthropogenic disturbance, making ecological study difficult. Therefore the first known species distribution model was created to predict the most likely areas of occurrence within the known range of these elusive animals. These models were then projected onto North America and evaluated against existing breeding programs. Additionally, the close proximity of the Gray Fossil Site and the discovery of the most complete fossil red panda specimens in the world allowed ecomorphological comparisons between the modern red panda, Ailurus fulgens …

An Epidemiological Study Of Obesity Among Asian Americans In California, United States, Shaoqing Gong

Electronic Theses and Dissertations

Obesity has reached epidemic levels in the United States (U.S.). Despite an increasing number of studies on obesity, a very few have addressed this debilitating condition among Asian American adults. The overall objective of this study is to utilize the latest cycles of the California Health Interview Survey (CHIS) to better understand obesity and identify its correlates among Asian Americans. The study population comprised Asian American adults aged 18 years or older from the CHIS with data pooled from the 2013 and 2014 survey years. Obesity (≥27.5 kg/m²) was defined using the World Health Organization (WHO) Asian body …

Quantifying The Structure Of Misfolded Proteins Using Graph Theory, Walter G. Witt

Electronic Theses and Dissertations

The structure of a protein molecule is highly correlated to its function. Some diseases such as cystic fibrosis are the result of a change in the structure of a protein so that this change interferes or inhibits its function. Often these changes in structure are caused by a misfolding of the protein molecule. To assist computational biologists, there is a database of proteins together with their misfolded versions, called decoys, that can be used to test the accuracy of protein structure prediction algorithms. In our work we use a nested graph model to quantify a selected set of proteins that …

Differentiating Between A Protein And Its Decoy Using Nested Graph Models And Weighted Graph Theoretical Invariants, Hannah E. Green

Electronic Theses and Dissertations

To determine the function of a protein, we must know its 3-dimensional structure, which can be difficult to ascertain. Currently, predictive models are used to determine the structure of a protein from its sequence, but these models do not always predict the correct structure. To this end we use a nested graph model along with weighted invariants to minimize the errors and improve the accuracy of a predictive model to determine if we have the correct structure for a protein.

Perception Of Factors That Facilitate Or Inhibit Associate Degree Completion At The Community College Level: A Case Study, Cathryn J-C Hughes Miss

Electronic Theses and Dissertations

The purpose of this study was to examine the perceptions of students, administrators, and faculty of one community college on the factors that facilitate or inhibit collegiate student success toward associate degree completion. Degree completion was defined as graduating with an associate degree. The following research questions guided this study:

1) What support systems or resources are in place at the community college to assist students with degree completion?

2) What factors in the college student success course at this community college facilitate or inhibit successful degree completion?

The case study was conducted in a single community college inNorth Carolina. …

Cranial Morphological Distinctiveness Between Ursus Arctos And U. Americanus, Benjamin James Hillesheim

Electronic Theses and Dissertations

Despite being separated by millions of years of evolution, black bears (Ursus americanus) and brown bears (Ursus arctos) can be difficult to distinguish based on skeletal and dental material alone. Complicating matters, some Late Pleistocene U. americanus are significantly larger in size than their modern relatives, obscuring the identification of the two bears. In the past, fossil bears have been identified based on differences in dental morphology or size. This study used geometric morphometrics to look at overall differences in cranial shape and used step-wise discriminant analysis to identify specific characters that distinguish cranial morphology between …