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Development Of Blended Polymeric Fibers To Alter The Release Of Peptide Targeting Porphyromonas Gingivalis., Sonali Suresh Sapare May 2018

Development Of Blended Polymeric Fibers To Alter The Release Of Peptide Targeting Porphyromonas Gingivalis., Sonali Suresh Sapare

Electronic Theses and Dissertations

Periodontitis is a chronic inflammatory disease that infects the tissues of the periodontium. It is estimated that 47.2% or 67.2 million American adults suffer from mild, moderate and severe periodontitis. Globally, 30-50% of the adult population is afflicted with periodontal disease, making it one of the most prevalent infectious diseases in the world. Therapeutics targeting P. gingivalis may be effective to alter periodontitis progression. However, the current treatment modalities that target critical pathogens to maintain host-biofilm homeostasis are limited, urging the development of specifically targeted therapeutics to limit P. gingivalis recolonization of the oral cavity after periodontal treatment and healing. …

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Therapeutic Potential Of A Plant-Made Cholera Toxin B Subunit Variant For The Treatment Of Ulcerative Colitis., Joshua Mark Royal May 2018

Therapeutic Potential Of A Plant-Made Cholera Toxin B Subunit Variant For The Treatment Of Ulcerative Colitis., Joshua Mark Royal

Electronic Theses and Dissertations

Cholera toxin B subunit (CTB) is a mucosal immunomodulatory protein that induces robust mucosal and systemic antibody responses. This well-known biological activity has been exploited in cholera prevention (as a component of Dukoral® vaccine) and vaccine development for decades. On the other hand, several studies have investigated CTB’s immunotherapeutic potential in the treatment of inflammatory diseases such as Crohn’s disease and asthma. Furthermore, we recently found that a plant-made variant of CTB (CTBp) could induce colon epithelial wound healing in mouse colitis models. In this thesis, it is revealed that the wound healing effects are unique to the plant-made variant, …

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Potential For Developing Run-Of-River Systems In Eastern Kentucky : A Gis Approach To Site Suitability., Thomas Jeffords May 2018

Potential For Developing Run-Of-River Systems In Eastern Kentucky : A Gis Approach To Site Suitability., Thomas Jeffords

Electronic Theses and Dissertations

Coal has a questionable future with the potential exhaustion of available coal. Alternate sources of energy production should be considered, such as hydropower dams, or the more environmentally friendly, Run of River (RoR) hydropower system. This study seeks to answer the question: What is the potential for RoR hydroelectric systems in Eastern Kentucky counties with significant decreases in coal production and employment? I hypothesize that GIS will identify suitable sites within Pike County for RoR systems. Site suitability for Eastern Kentucky was assessed and determined that Pike County did not have a suitable physical environment for RoR systems. The power …

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African American Grandmothers As The Black Matriarch : You Don't Live For Yourself., Tanisha Nicole Stanford May 2018

African American Grandmothers As The Black Matriarch : You Don't Live For Yourself., Tanisha Nicole Stanford

Electronic Theses and Dissertations

This study examined historical and contemporary roles of African American grandmothers within the familial system, and their socio-psychological experiences. The primary method of data collection was semi-structured, conversational style interviews with an oral history aspect. There were six grandmothers interviewed, two from the midwest region of the United States, and four from the southern region. The findings reveal stories that corroborate with the literature on the role of women in African American families and that of the Black matriarch, considering their strength are not inherent but necessary. They are not born matriarchs or strong black women, they become that person …

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Inflammasome Independent Leukotriene-B4 Production Drives Crystalline Silica Induced Sterile Inflammation., Bindu Hegde May 2018

Inflammasome Independent Leukotriene-B4 Production Drives Crystalline Silica Induced Sterile Inflammation., Bindu Hegde

Electronic Theses and Dissertations

Silicosis is an irreversible lung inflammatory disease caused by chronic exposure to crystalline silica (CS) and is one of the most prevalent occupational diseases worldwide. Lipid chemoattractant Leukotriene B4 (LTB4) plays an important role in neutrophilic inflammation that drives silicosis and promotes lung cancer. Previous studies in our laboratory have demonstrated that CS-induced neutrophilic inflammation and lung tumor burden in K-rasLA1 mice is abrogated in the LTB4-receptor deficient mice. Another pathway whose importance is well studied in the progression of silicosis is the Nalp3 inflammasome pathway. Studies have shown inflammasome- dependent IL-1b to be …

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There Is Water In The World For Us : The Environmental Theories Of Alice Walker., Janae Lewis Hall May 2018

There Is Water In The World For Us : The Environmental Theories Of Alice Walker., Janae Lewis Hall

Electronic Theses and Dissertations

The emergence of African-American Environmental thought responds to the ongoing erasure of Black experiences and their perspectives on nature. Mainstream environmentalism maintains a legacy of perceived innocence and incorruptibility towards the land, while Black Environmentalism demonstrates the limitations of that ideology. Limitations include the erasure of history in regards to stealing land from Indigenous people, the brutality of slavery, legalized lynching, forced removal from the land, exploitation in sharecropping, destruction of sacred lands, heavy pollution in urban centers, and harmful environmental policies. For Black and Indigenous peoples, it is impossible to view American soil as innocent. This project surveyed the …

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Gender Boundary Negotiation Within The U.S. Immigrant/Refugee Resettlement : How Transnational Bridge-Building Matters., Telesphore Kagaba May 2018

Gender Boundary Negotiation Within The U.S. Immigrant/Refugee Resettlement : How Transnational Bridge-Building Matters., Telesphore Kagaba

Electronic Theses and Dissertations

For the past several decades, social research about U.S. immigrant and refugee resettlement has focused on some of the ways federal and non-governmental organizations help immigrants and refugees integrate into mainstream society. Little research, if any, has explored how giving voice to immigrants and refugees is done in bridging transnational gaps and strengths in the process of their adjustment to the host country. This dissertation is grounded within transnational theory (Avenarius 2012, Levitt 2011, Peteet 1997) that argues that immigrants and refugees bring their experiences and cultures from their home countries, time in refugee camps, and other often traumatic experiences …

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Functional And Structural Impact Of The Loss Of The Leucine-Rich Repeat Protein Lrit1 In The Mouse Retina., Catherine Ann Cobb May 2018

Functional And Structural Impact Of The Loss Of The Leucine-Rich Repeat Protein Lrit1 In The Mouse Retina., Catherine Ann Cobb

Electronic Theses and Dissertations

Mutations in genes encoding the leucine-rich repeat (LRR) proteins nyctalopin and LRIT3 lead to complete congenital stationary night blindness because they are critical to depolarizing bipolar cell function in the retina. LRIT3 has two closely related family members, LRIT1 and LRIT2. In silico analyses of publicly available RNA-Seq data showed that Lrit1 was highly expressed in the retina. Here I describe the expression pattern and impact of loss of LRIT1 on retinal function. To enable these studies, we used CRISPR/Cas9 technology to create an Lrit1-/- mouse line. Retinal morphology and morphometry analyses showed no gross changes in retinal structure …

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The Styles Of Criminology : Writing Among The Stars., Ethan Maxwell Higgins May 2018

The Styles Of Criminology : Writing Among The Stars., Ethan Maxwell Higgins

Electronic Theses and Dissertations

This study investigates how criminological writing style is "a [more] complicated terrain" than we realize in criminology (Butler, 1990, p. xix). Data is drawn from interviews with forty of the one hundred most influential criminological writers (Walters, 2015) to explore perceptions of good writing in the field, namely in describing writing practices, norms and values. Thus, the findings of this study come in three chapters. The first two result chapters explore the terrain of writing in criminology by presenting perceptions of best practices and values of good writing. The third results chapter then examines these perceptions within a lens of …

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Assessment And Optimization Of Environmental Systems Using Data Analysis And Simulation., Milad Ebrahimi May 2018

Assessment And Optimization Of Environmental Systems Using Data Analysis And Simulation., Milad Ebrahimi

Electronic Theses and Dissertations

For most environmental systems, specifically wastewater treatment plants and aquifers, a significant number of performance data variables are attained on a time series basis. Due to the interconnectedness of the variables, it is often difficult to assess over-arching trends and quantify temporal operational performance. The objective of this research study was to provide an effective means for comprehensive temporal evaluation of environmental systems. The proposed methodology used several multivariate data analyses and statistical techniques to present an assessment framework for the water quality monitoring programs as well as optimization of treatment plants and aquifer systems. The developed procedure considered the …

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Breaking With Tradition(?) : Female Representations Of Heroism In Old English Poetry., Kathryn A. Green May 2018

Breaking With Tradition(?) : Female Representations Of Heroism In Old English Poetry., Kathryn A. Green

Electronic Theses and Dissertations

For the Anglo-Saxons, strength, bravery, and the willingness to put oneself in harm’s way for king and kingdom were not only part of contemporary society but recurring themes in Old English literature. Poems like Beowulf and The Battle of Maldon reinforce the important bond between lord and retainer and the heroic ethos key to that relationship. Women were not historically part of this relationship and, therefore, not subject to the heroic code in the same way; consequently, they are rarely seen as anything more than conventional mothers, queens, wives, sisters, daughters, and virgins, all identified by their relationships to men, …

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The Effects Of Habitat And Connectivity On Tropical Ant Ecology And Behavior., Benjamin Jacob Adams May 2018

The Effects Of Habitat And Connectivity On Tropical Ant Ecology And Behavior., Benjamin Jacob Adams

Electronic Theses and Dissertations

This dissertation explores the role that physical connections among and within habitats play in determining the local diversity and behaviors of cursorial organisms. Understanding the processes that regulate local community assembly and that contribute to the maintenance of species diversity is a cornerstone of community ecology. Ants are a useful model for studies of local communities because they are abundant and fulfill a variety of ecological roles. Chapter one of the dissertation examines the how lianas (woody vines) function as connective structures among tree crowns and how these connections affect arboreal ant community structure within a tropical forest canopy. Trees …

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Divergent Evolution Of Di-Lysine Er Retention Vs. Farnesylation Motif-Mediated Anchoring Of The Ankb Virulence Effector., John David Perpich May 2018

Divergent Evolution Of Di-Lysine Er Retention Vs. Farnesylation Motif-Mediated Anchoring Of The Ankb Virulence Effector., John David Perpich

Electronic Theses and Dissertations

Legionella pneumophila is an aquatic organism capable of intracellular replication within a wide range of protozoan hosts and within human macrophages where it causes Legionnaires’ Disease. L. pneumophila manipulates a variety of host cell processes by translocating, into the host cell cytosol, more than 300 effector proteins via the type IVB secretion system (T4SS). The AnkB effector from the AA100/130b strain (AnkB-AA100) contains a C-terminal CaaX motif that rapidly becomes farnesylated upon translocation into the host cell and anchored to the cytosolic face of the Legionella-containing vacuole (LCV) membrane, which is essential for intracellular replication. We show that a …

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A New Surgical Approach For O-C2 Fusion, Solving The Problem Of Dysphagia., M. Kathryn Mcclure May 2018

A New Surgical Approach For O-C2 Fusion, Solving The Problem Of Dysphagia., M. Kathryn Mcclure

Electronic Theses and Dissertations

The fusion of the second cervical vertebrae to the occipital bone (O-C2 fusion) for head stabilization can result in postoperative dysphagia and dyspnea, negatively impacting the patient’s quality of life. Currently, the O-C2 angle is used for head placement, which may not place the head neutrally. We hypothesize that aligning the external auditory meatus with midline of the C2 will reduce oropharyngeal stenosis, reducing dysphagia. One male patient with poor swallow quality of life who required a revision of a previous O-C2 surgery was evaluated via videofluoroscopy and completed the standard swallow quality of life questionnaire (SWAL-QOL) before and after …

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Factors Influencing The Retention Of Women Faculty In Stem Disciplines., Carrie J. Christensen May 2018

Factors Influencing The Retention Of Women Faculty In Stem Disciplines., Carrie J. Christensen

Electronic Theses and Dissertations

Low numbers of women faculty in STEM (science, technology, engineering and mathematics) disciplines continues to be a concern in higher education. Even though completion of STEM degrees by women has increased in many disciplines, increases in the number of women faculty have not been seen. Additionally, women continue to leave faculty positions at twice the rate of men. In order to remain globally competitive, the US needs to retain a diverse STEM professoriate. This dissertation examined the factors influencing the retention of women faculty in STEM disciplines and their over-representation in non-research intensive institutions. The analysis was broken into two …

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Network Science Algorithms For Mobile Networks., Heba Mohamed Elgazzar May 2018

Network Science Algorithms For Mobile Networks., Heba Mohamed Elgazzar

Electronic Theses and Dissertations

Network Science is one of the important and emerging fields in computer science and engineering that focuses on the study and analysis of different types of networks. The goal of this dissertation is to design and develop network science algorithms that can be used to study and analyze mobile networks. This can provide essential information and knowledge that can help mobile networks service providers to enhance the quality of the mobile services. We focus in this dissertation on the design and analysis of different network science techniques that can be used to analyze the dynamics of mobile networks. These techniques …

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Soil Ecology Of The Exotic Dune Grass Leymus Arenarius., Matthew L. Reid May 2018

Soil Ecology Of The Exotic Dune Grass Leymus Arenarius., Matthew L. Reid

Electronic Theses and Dissertations

Exotic plants can alter the structure and function of native communities both above- and belowground, which is particularly important in primary successional sand dune ecosystems where plant-soil interactions are critical drivers of successional dynamics. In Great Lakes sand dunes, the native foundation grass Ammophila breviligulata dominates early in succession until an accumulation of detrimental soil organisms causes its die-back of the grasses. Die-back of this native foundation species can be slowed by mutualistic interactions between plants and mycorrhizal fungi. Changes to the plant community resulting from invasion, and subsequent effects on the soil community have the potential to disrupt the …

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Multi Self-Adapting Particle Swarm Optimization Algorithm (Msapso)., Gerhard Koch May 2018

Multi Self-Adapting Particle Swarm Optimization Algorithm (Msapso)., Gerhard Koch

Electronic Theses and Dissertations

The performance and stability of the Particle Swarm Optimization algorithm depends on parameters that are typically tuned manually or adapted based on knowledge from empirical parameter studies. Such parameter selection is ineffectual when faced with a broad range of problem types, which often hinders the adoption of PSO to real world problems. This dissertation develops a dynamic self-optimization approach for the respective parameters (inertia weight, social and cognition). The effects of self-adaption for the optimal balance between superior performance (convergence) and the robustness (divergence) of the algorithm with regard to both simple and complex benchmark functions is investigated. This work …

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An Agent Based Model To Assess Crew Temporal Variability During U.S. Navy Shipboard Operations, Kevin Muhs May 2018

An Agent Based Model To Assess Crew Temporal Variability During U.S. Navy Shipboard Operations, Kevin Muhs

Electronic Theses and Dissertations

Understanding the factors that affect human performance variability as well as their temporal impacts is an essential element in fully integrating and designing complex, adaptive environments. This understanding is particularly necessary for high stakes, time-critical routines such as those performed during nuclear reactor, air traffic control, and military operations. Over the last three decades significant efforts have emerged to demonstrate and apply a host of techniques to include Discrete Event Simulation, Bayesian Belief Networks, Neural Networks, and a multitude of existing software applications to provide relevant assessments of human task performance and temporal variability. The objective of this research was …

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Tissue Engineered Micro And Macrovasculature Utilizing Stromal Vascular Fraction., Joseph Samir Zakhari May 2018

Tissue Engineered Micro And Macrovasculature Utilizing Stromal Vascular Fraction., Joseph Samir Zakhari

Electronic Theses and Dissertations

This dissertation describes the use of stromal vascular fraction to tissue engineer 3D microvasculature and macrovasculature. Stromal vascular fraction is an easily isolatable cell source from adipose tissue depots. It has demonstrated remarkable potential both in vitro and in vivo for forming microcirculation capable of perfusion upon implantation. SVF is clinically utilized as a therapeutic cell source for anti-inflammation for osteoarthritis and is being studied for ischemic tissue application to stimulate revascularization. The work described herein is divided within four chapters. Chapter I provides an introductory overview and lists the aims and hypothesis for the dissertation. Chapter II describes experiments …

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Optimizing High-Speed Exercise Performance : Effect Of Load., Ling Bai May 2018

Optimizing High-Speed Exercise Performance : Effect Of Load., Ling Bai

Electronic Theses and Dissertations

The purpose of this study was to identify an optimal load for the knee extension exercise done with Impulse (Newnan, GA). Forty-one women and twenty-nine men made six laboratory visits, including two familiarization sessions of the knee extension exercise and four real workout sessions which entailed four 30-second exercise sets, separated by 120-second rests, against four different loads (0Kg, 3.41Kg, 5.68Kg, 7.95Kg). A Latin Squares design was used to counterbalance the sequence of the sets. Average force (AF), peak force (PF), total work (TW), Impulse value, and the number of knee extension repetitions were each analyzed with a 2 (gender) …

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Characterizing The Virulence Factor Yape In Yersinia Pestis., Tiva Templeton Vancleave May 2018

Characterizing The Virulence Factor Yape In Yersinia Pestis., Tiva Templeton Vancleave

Electronic Theses and Dissertations

Yersinia pestis is the causative agent of bubonic plague and is primarily transmitted by fleas. Upon infection, the bacteria rapidly travel to the regional lymph nodes causing inflammation and cellulitis in these tissues (referred to as buboes). Two outer membrane proteins, YapE and Pla, have been implicated to have roles in dissemination to the lymph nodes. Their adhesive properties have shown that they are able to interact with host macrophages thereby increasing their ability to disseminate to regional lymph nodes. More recently, we have shown that YapE is cleaved by another virulence factor important for lymph node colonization, Pla, to …

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Region Based Gene Expression Via Reanalysis Of Publicly Available Microarray Data Sets., Ernur Saka May 2018

Region Based Gene Expression Via Reanalysis Of Publicly Available Microarray Data Sets., Ernur Saka

Electronic Theses and Dissertations

A DNA microarray is a high-throughput technology used to identify relative gene expression. One of the most widely used platforms is the Affymetrix® GeneChip® technology which detects gene expression levels based on probe sets composed of a set of twenty-five nucleotide probes designed to hybridize with specific gene targets. Given a particular Affymetrix® GeneChip® platform, the design of the probes is fixed. However, the method of analysis is dynamic in nature due to the ability to annotate and group probes into uniquely defined groupings. This is particularly important since publicly available repositories of microarray datasets, such as ArrayExpress and NCBI’s …

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Effect Of Porcelain Thickness And Translucency On The Final Shade Of Ips E.Max Cad Overlaying A Dark Substrate., Peter F. Alena May 2018

Effect Of Porcelain Thickness And Translucency On The Final Shade Of Ips E.Max Cad Overlaying A Dark Substrate., Peter F. Alena

Electronic Theses and Dissertations

IPS e.max is a very esthetic lithium disilicate used in dental ceramics. Unfortunately, it can unintentionally change color after cementation with resin cements. The purpose of this study is to better understand how combining thickness and translucency affects the post-cementation shade of IPS e.max CAD overlaying a dark substrate, thereby enabling clinicians to choose more appropriate techniques and materials. IPS e.max CADs (A1 shade) had either a low translucency (LT) or high translucency (HT), and cut with 0.3mm, 0.5mm, and 1.0mm thicknesses. Each sample overlaid a resin cement and dark substrate, a spectrophotometer helped derive the color difference (ΔE*ab), and …

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Horse Racing Prediction Using Graph-Based Features., Mehmet Akif Gulum May 2018

Horse Racing Prediction Using Graph-Based Features., Mehmet Akif Gulum

Electronic Theses and Dissertations

This thesis presents an applied horse racing prediction using graph based features on a set of horse races data. We used artificial neural network and logistic regression models to train then test to prediction without graph based features and with graph based features. This thesis can be explained in 4 main parts. Collect data from a horse racing website held from 2015 to 2017. Train data to using predictive models and make a prediction. Create a global directed graph of horses and extract graph-based features (Core Part) . Add graph based features to basic features and train to using same …

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A Model To Study The Effects Of Whole Life Chronic Exposure To Arsenic Or Cadmium On The Development Of Adult Metabolic Syndrome : Initial Characterization Of Hepatic Changes., Jamie L. Young May 2018

A Model To Study The Effects Of Whole Life Chronic Exposure To Arsenic Or Cadmium On The Development Of Adult Metabolic Syndrome : Initial Characterization Of Hepatic Changes., Jamie L. Young

Electronic Theses and Dissertations

Metabolic syndrome (MetS) is a group of diseases affecting < 30% of adults. Although obesity is a major risk for the development of MetS, it does not account for all cases, suggesting contribution of other risk factors. We hypothesized that early life exposure to arsenic (As) or cadmium (Cd) may represent such a risk. The purpose of this study was to characterize a model to discern the effects of early life exposures to Cd and As on high fat diet (HFD)-induced MetS. Adult C57BL/6J mice were exposed to control or metals containing drinking water. Pregnant dams and offspring were continuously exposed to the same toxicants as their parents. At weaning, offspring were fed LFD or HFD and sacrificed 10 or 24 weeks later. Metal exposure caused time- and sex-dependent alterations in HFD-induced variables of liver damage. The initial results suggest that these toxicants enhanced obesity-induced liver injury.

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Investigation Of Cardiovascular Tissue Following 56fe-Radiation And Potential Countermeasure Effectiveness., Alexandra R Decarlo May 2018

Investigation Of Cardiovascular Tissue Following 56fe-Radiation And Potential Countermeasure Effectiveness., Alexandra R Decarlo

Electronic Theses and Dissertations

Exposure to ionizing radiation is an important medical concern because it can lead to health problems including cancer and cardiovascular issues. In space, astronauts are exposed to ionizing radiation that is not experienced by those who remain on Earth. This radiation can cause health problems such as cardiovascular disease. One way this can happen is through the creation of reactive oxygen species, which can activate TGF-β1, contributing to fibrosis or other cardiovascular problems. Antioxidants can be a potential pharmacological mitigator of these excess reactive oxygen species because of their ability to neutralize reactive oxygen species. One such antioxidant with potential …

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Plga-Modified Nanoparticles For The Treatment Of Hypo-Vascularized Hpv-Related Cervical Cancers., Lee B. Sims May 2018

Plga-Modified Nanoparticles For The Treatment Of Hypo-Vascularized Hpv-Related Cervical Cancers., Lee B. Sims

Electronic Theses and Dissertations

A major challenge associated with delivery of active agents in the female reproductive tract (FRT) is the ability of agents to efficiently diffuse through the cervicovaginal mucosa (CVM) and reach the underlying sub-epithelial immune cell layer and vasculature. A variety of drug delivery vehicles have been employed to improve the delivery of agents across the CVM and offer the capability to increase the longevity and retention of active agents to treat infections of the female reproductive tract. Nanoparticles (NPs) have been shown to improve retention, diffusion, and cell-specific targeting via specific surface modifications, relative to other delivery platforms. In particular, …

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Enhancing Performance Of Ionic Liquid Property Prediction With Molecular Dynamics., Trisha H. Patel May 2018

Enhancing Performance Of Ionic Liquid Property Prediction With Molecular Dynamics., Trisha H. Patel

Electronic Theses and Dissertations

Molecular dynamics have been used to predict thermodynamic and transport properties of eight room-temperature ionic liquids. Simulation parameters including box size and van der Waals cutoffs were varied. The density, heat capacity, and self-diffusion coefficients of the ionic liquids were computed and compared to experimental data and to previously published simulations. Predicted properties were generally close to their experimentally observed values. It was determined that the prediction of ionic liquid properties via molecular dynamics simulations could be accelerated several-fold by using less stringent integration parameters and smaller simulation sizes. The properties of density and heat capacity did not change significantly …

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Comparative Analysis Of Survival And Decay Of Fecal Indicator Bacteria In Bovine Feces And Freshwater Microcosms, Reem Tariq May 2018

Comparative Analysis Of Survival And Decay Of Fecal Indicator Bacteria In Bovine Feces And Freshwater Microcosms, Reem Tariq

Electronic Theses and Dissertations

Agricultural runoff can carry FIB that can pollute surface waters through the soil matrix. This study was designed to inspect the impact of temperature and matrix on the survival of FIB. The FIB were routinely enumerated over an 18-day period from fecal samples and freshwater microcosms maintained at 4oC, 22oC, and 35oC. It was found that the FIB studied underwent a primary growth of up to 1-log10 to 3-log10, highlighting the weakness of conventional FIB as indicators of pathogen contamination. The concentrations of FIB in the water phase were found to be significantly greater than those observed in the fecal …

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