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Role Of Atm In T Cell Dysfunction During Chronic Viral Infections, Juan Zhao

Electronic Theses and Dissertations

Hepatitis C virus (HCV) or human immunodeficiency virus (HIV) infection leads to a phenomenon of inflammaging, in which chronic infection or inflammation induces an immune aged phenotype with T cell dysfunction. Thus, HCV or HIV infection has been deemed as a model to study the mechanisms of T cell infammaging and viral persistence in humans. In this dissertation, T cell homeostasis, DNA damage and repair machineries were investigated in patients with chronic HCV or HIV infection at risk for inflammaging. We found a significant depletion in CD4 T cells, which was correlated with their apoptosis in chronically HCV/HIVinfected patients, compared …

Adverse Childhood Experiences, Homeless Chronicity, And Age At Onset Of Homelessness, Joseph T. Tucciarone Jr.

Electronic Theses and Dissertations

Childhood adversity is associated with numerous negative outcomes across multiple domains, including mental and physical health, interrelationships, and social functioning. Notably, research suggests that childhood adversity has a dose-response relationship with these outcomes; that is, greater numbers of adverse experiences in childhood are associated with worse outcomes. These outcomes overlap with many risk factors of homelessness. This study sought to address two questions: 1) Does a dose-response relationship exist between childhood adversity and chronic homelessness? 2) Does childhood adversity negatively predict the age at which homelessness first occurs? Adults experiencing homeless who are accessing homeless services in the Tri-Cities area …

Religious Attendance, Surrender To God, And Suicide Risk: Mediating Pathways Of Feeling Forgiven By God And Psychopathology, Kelley Pugh

Electronic Theses and Dissertations

Suicide is a national public health concern, and college students may be at increased risk. Symptoms of psychopathology (i.e., stress, anxiety, and depression) may contribute to risk, whereas religiosity (i.e., religious attendance, surrendering to God, and feeling forgiven by God) may reduce risk. Students from a rural southeastern university (N=249) completed self-report measures. Serial mediation analyses indicate that attendance and surrender to God are inversely- predictive of suicide risk, both directly and through the indirect pathways of feeling forgiven by God (1st order mediator) and psychopathology (2nd order mediators). In all models, specific indirect effects occurred through feeling forgiven by …

Performing Bernarda: Activating Power And Identity, Ana Martinez Medina

Electronic Theses and Dissertations

The musical Bernarda Alba tells the story of a woman who is confined within the heavily patriarchal and Catholic society that was 1930s Spain. Because of this, I thought it the perfect arena to explore power dynamics on stage. My thesis will explore status, hierarchies, relationships, and identity via the stolid matriarchal character Bernarda Alba. Through analyzing the playwright's words, fleshing out the character, and exploring the character's relationships with others in rehearsal, I have studied how to activate status on stage. There are many sociology theories and psychological studies that can be applied to theatre-making in order to create …

Gender Performance In Shakespeare's Twelfth Night, Amanda Lee

Electronic Theses and Dissertations

Cross-dressing is a recurrent theme in Shakespeare's comedies, and the theatrical trend of gender bending casting has added an extra layer of complexity to performing his work. How does the gender of the actor affect the performance of a role in Shakespeare? How does it affect the perception of the role, and how can an actor utilize that perception to connect more fully with the audience? How does the female perspective illuminate hitherto unexplored elements of Shakespeare's text and characters? I was inspired by Orlando Shakes' all male production of Twelfth Night to research gender theory in relation to classical …

Telomeric Dna Damage And Repair Machineries In Hiv Infection, Lam Nguyen

Electronic Theses and Dissertations

In this thesis, we investigated T cell homeostasis and DNA damage repair machineries in HIV infection. We found that the frequencies of CD4T cells were low, which is associated with cell apoptosis in HIV patients compared to healthy subjects. Importantly, these events were closely correlated to the increase in T cell exhaustion, senescence, DNA damage, and telomere attrition. Mechanistically, while the DNA damage sensors Mer11, Rad50, and NBS1 (MRN) complexes remained intact, the ataxia-telangiectasia mutated (ATM) kinase and its downstream checkpoint kinase 2 (CHK2) were significantly inhibited during HIV infection. Additionally, telomeric repeat-binding factor 2 (TRF2) that functions to protect …

The Prevalence And Context Of Adult Female Overweight And Obesity In Sub-Saharan Africa, Ifeoma Ozodiegwu

Electronic Theses and Dissertations

Adult women bear a disproportionate burden of overweight and obesity in Sub-Saharan Africa (SSA). Precise information to understand disease distribution and assess determinants is lacking. Therefore, this dissertation aimed to: (i) analyze the prevalence of adult female overweight and obesity combined in lower-level administrative units; (ii) analyze the effect modification of educational attainment and age on the association between household wealth and adult female overweight and obesity; (iii) synthesize qualitative research evidence to describe contextual factors contributing to female overweight and obesity at different life stages. Bayesian and logistic regression models were constructed with Demographic and Health Survey (DHS) data …

A Comparison Of Standard Denoising Methods For Peptide Identification, Skylar Carpenter

Electronic Theses and Dissertations

Peptide identification using tandem mass spectrometry depends on matching the observed spectrum with the theoretical spectrum. The raw data from tandem mass spectrometry, however, is often not optimal because it may contain noise or measurement errors. Denoising this data can improve alignment between observed and theoretical spectra and reduce the number of peaks. The method used by Lewis et. al (2018) uses a combined constant and moving threshold to denoise spectra. We compare the effects of using the standard preprocessing methods baseline removal, wavelet smoothing, and binning on spectra with Lewis et. al’s threshold method. We consider individual methods and …

The Effects Of Two Novel Anti-Inflammatory Compounds On Prepulse Inhibition And Neural Microglia Cell Activation In A Rodent Model Of Schizophrenia, Heath W. Shelton

Electronic Theses and Dissertations

Recent studies have shown elevated neuroinflammation in a large subset of individuals diagnosed with schizophrenia. A pro-inflammatory cytokine, tumor necrosis factor-alpha (TNFα), has been directly linked to this neuroinflammation. This study examined the effects of two TNFα modulators (PD2024 and PD340) produced by our collaborators at P2D Bioscience, Inc., to alleviate auditory sensorimotor gating deficits and reduce microglial cell activation present in the polyinosinic:polycytidylic (Poly I:C) rodent model of schizophrenia. Auditory sensorimotor gating was assessed using prepulse inhibition and microglial activation was examined and quantified using immunohistochemistry and confocal microscopy, respectively. Both PD2024 and PD340 alleviated auditory sensorimotor gating deficits …

The Relations Of Religiosity, Social Support, And Acculturation Attitudes Among Refugees, Carolyn H. Casada

Electronic Theses and Dissertations

Refugees are entering the United States in increasing numbers. Identifying factors that promote successful acculturation is an important task for those working to help refugees. As religiosity and social support have previously been linked to better mental health outcomes in refugees, they should be considered when examining acculturation. Using the Duke University Religious Index, the Multidimensional Scale of Perceived Social Support, and the Acculturation Attitudes Scale, this study examined the relations between religiosity, perceived social support, and acculturation strategies. Additional variables examined were number of migrations, language of religious services currently attended, and religious services demographics. Hypotheses were that scores …

Culturally Responsive Training For Secondary English Language Teachers, Tina Mccorkle

Electronic Theses and Dissertations

In this quantitative study, I investigated the effectiveness of a training intervention program to positively impact secondary teacher attitudes and perceptions of culturally responsive teaching (CRT). The study is relevant in Alvin Independent School District given the demographic shift resulting in an increase in Hispanic students and students learning English as a second language. The purpose of the study was to investigate teacher attitudes and perceptions of CRT to determine if there was a statistically significant difference according to pre- and post-survey data in order to evaluate the effectiveness of the CRT program intervention. The CRT research from Ladson-Billings (1992) …

The Effect Of Image Repair On Perceived Relationship Satisfaction After Infidelity, Sydney M. Shields

Electronic Theses and Dissertations

In the wake of infidelity, romantic partners must decide how to move beyond the act of betrayal. Although infidelity can be concealed, sometimes others learn of the infidelity, which may impel a cheater to take steps to repair his or her image. This study examined the use of image repair on social media (Facebook), specifically the tactic of admitting responsibility, in the wake of infidelity. After reading a vignette describing infidelity by the male partner in a heterosexual relationship and viewing a social media post from the cheater, participants answered a series of questions about the couple’s perceived relationship satisfaction. …

Faculty Socialization Of Graduate Students’ Attitudes And Perceptions Toward The Irb, Elisa Jolls

Electronic Theses and Dissertations

Graduate student mistrust and fear of the institutional review board (IRB) is an issue that is commonly encountered at academic institutions. Research has shown that students emulate and are vulnerable to assuming the norms of faculty, which is supported by research that shows that students who have difficult relationships with the IRB often have faculty mentors who also have difficult relationships with the IRB. The purpose of this qualitative case study was to explore how novice researchers’ perceptions of the IRB changed through their research submission and IRB interactions required during the IRB approval process, as well as to identify …

Nano-Biophysical Approaches For Assessing Nanoparticle Interactions With Biological Systems, Zachary Untracht

Electronic Theses and Dissertations

Understanding interactions between nanoparticles and biological systems is fundamental for the development of emerging nano-biotechnology applications. In this thesis, I present an investigation of zinc oxide (ZnO) nanoparticles interactions with biomolecules in two separate studies. The first section of my thesis covers tracking and detection of ZnO nanoparticles using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). ZinkicideTM is a bactericidal ZnO nanoparticle which has been developed for agriculture. The characterization of Zinkicide in biological media and in solution has been difficult due to its high dispersibility and ultra-small size. SDS-PAGE is considered a golden standard for protein qualitative interpretations. In …

Nike's Corporate Social Advocacy (Csa) Practices As Related To Strategic Issues Management (Sim) And Threats To Organizational Legitimacy, Eve Heffron

Electronic Theses and Dissertations

This research examined how corporate social advocacy (CSA) and corporate social responsibility (CSR) efforts impacted perceptions of authenticity. Using an experimental survey, participants were randomly exposed to Nike's actions related to the Black Lives Matter (BLM) movement via mock-online news articles. Participants completed a survey that contained Likert-type scale items regarding attitudes (perceived corporate intent, perceived authenticity, brand trust, and brand credibility) and behavioral intentions (word of mouth intentions (WOM), and purchase intention (PI)). Results indicated that positive attitudes significantly increased when Nike implemented an action step after taking a public stance on a controversial social-political issue. Further, results revealed …

The Unacceptable Rise: An Investigation Of School Shootings In The United States, Tracy Gulliford

Electronic Theses and Dissertations

This research study examined teachers' understanding of risk factors and characteristics of potential perpetrators of school shootings, as well as the steps to take when they are identified in students. Participants completed a survey that contained Likert-type scale items regarding self efficacy and open-ended questions regarding school violence experiences. A mixed methods analysis revealed three primary conclusions. First, teachers have high levels of self-efficacy regarding the importance of and ability to deal effectively with troubled students. This conclusion suggests that teachers do internalize the relevance of this issue to them. Second, this study revealed a gap in terms of the …

Brief Behavioral Health Intervention Program For Patients With Stable Coronary Artery Disease, Chelsea Wiener

Electronic Theses and Dissertations

Healthy eating, physical activity, stress management, and smoking cessation are widely recognized as essential for preventing and treating coronary artery disease (CAD). Research on lifestyle programs for patients with CAD has largely focused on long-term interventions (e.g., several months to one-year in duration). Further, many studies have recruited patients immediately post-cardiac event. By contrast, evaluation of brief lifestyle interventions for stable patients treated in outpatient cardiology is lacking. The present study evaluated the feasibility, acceptability, and efficacy of a 3-session behavioral health lifestyle program for patients with stable CAD being treated in an outpatient cardiology clinic. Thirty-three patients were randomized …

Fabrication Of Polyelectrolyte Nanoparticles Through Hydrophobic Interaction, Ruginn Porce Catarata

Electronic Theses and Dissertations

Anticancer drugs like gemcitabine (GEM) are used to treat cancers such as, pancreatic ductal adenocarcinoma (PDAC). However, the use of free gemcitabine yields challenges including cytotoxicity to healthy cells and poor circulation time. By encapsulating GEM in nanoparticles these challenges can be overcome. In this study poly(acrylic acid) (PAA)-GEM nanoparticles are fabricated by coupling GEM onto PAA. The particle formation is driven by the hydrophobic interaction of GEM, which collects in the core of the nanoparticle, forming a PAA shell. The nanoparticles were optimized by studying the PAA/GEM ratio and pH during fabrication. Characteristics of the nanoparticles including size, morphology …

Development Of 3d Printed And 3d Metal-Based Micro/Nanofabricated, And Nano-Functionalized, Microelectrode Array (Mea) Biosensors For Flexible, Conformable, And In Vitro Applications, Charles Didier

Electronic Theses and Dissertations

Emerging fields such as "Organs on a Chip", disease modeling in vitro, stem cell manufacturing and wearable bioelectronics are demanding rapid development of 3D Microelectrode Arrays (MEAs) for electrical interfacing with biological constructs. The work reported in this thesis focuses on two developmental tracks: "Dynamic 3D MEAs" and metal microfabrication for 3D MEAs. In the first part of the thesis, we explore the capabilities and limitations of 3D printed microserpentines (µserpentines) and utilize these structures to develop dynamic 3D microelectrodes. Analytical modeling of µserpentines flexibility followed by integration into a flexible Kapton® package and PDMS insulation are demonstrated. These 3D …

Supervised Machine Learning Techniques For Short-Term Load Forecasting, Harish Amarasundar

Electronic Theses and Dissertations

Electric Load Forecasting is essential for the utility companies for energy management based on the demand. Machine Learning Algorithms has been in the forefront for prediction algorithms. This Thesis is mainly aimed to provide utility companies with a better insight about the wide range of Techniques available to forecast the load demands based on different scenarios. Supervised Machine Learning Algorithms were used to come up with the best possible solution for Short-Term Electric Load forecasting. The input Data set has the hourly load values, Weather data set and other details of a Day. The models were evaluated using MAPE and …

Rasch Analysis Of Relational Well-Being Within The National Survey Of Adoptive Parents Of 2007: A Comparison Of Multidimensional, Consecutive, And Unidimensional Approaches To Measure Construction, Michael A. Furno

Electronic Theses and Dissertations

A unidimensional Rasch approach was used to explore whether the data collected through the National Survey of Adoptive Parents of 2007 (NSAP) for the well-being items represented a single latent construct and to establish a base model for comparison. A consecutive approach was then used as an exploratory tool to draw out potential multiple dimensions. Finally, multidimensional item response theory (MIRT) was used to confirm the results of the consecutive approach findings while comparing with the unidimensional baseline. Items within the survey were evaluated for scale function as well as invariance.

The comparison of three approaches (unidimensional, combined consecutive, and …

Divergence Of Threespine Stickleback That Differ In Nuptial Coloration, Clara Sophie Jenck

Electronic Theses and Dissertations

Recent research has led to a much better understanding of the evolutionary processes that mold and structure variation within and among populations. How populations diverge at the genome-wide level and how polymorphism is maintained within a species, however, remains unclear. We address these questions with two freshwater color morphs, red and black, of the threespine stickleback fish (Gasterosteus aculeatus) from the northwest United States, in which a shift from red to black nuptial coloration occurred in several locations following glacial retreat. We measured phenotypic variation in a suite of traits and used next generation sequencing to characterize within-species and among-morph …

Amelioration Of Alzheimer's Disease Pathology In 12-Month-Old Happ(Sweind) Transgenic Mice After Treatment With A Cysteine Rich Whey Supplement, Immunocal®, Srivalli Puttagunta

Electronic Theses and Dissertations

Deficits in Reelin expression and signaling play a pathogenic role in Alzheimer’s disease (AD). Thus, strategies aimed at correcting Reelin deficits may provide a novel therapeutic approach to treating AD. The cysteine-rich, whey protein supplement, Immunocal®, has recently been shown to rescue Reelin expression in a mouse model of Schizophrenia. Given that Reelin-expressing neurons of the entorhinal cortex region are a highly vulnerable population of cells that are lost early in AD, we examined the effects of Immunocal® in the hippocampal-entorhinal cortex formation in a mouse model of AD. Glutathione levels and Reelin expression in the hippocampal-entorhinal cortex formation (entorhinal …

"En El Poder Del Espíritu": A Qualitative Research Study On Social Ethics/Theology Among U.S. Latina/O Pentecostals, Néstor A. Gómez Morales

Electronic Theses and Dissertations

Are most US Latina/o pentecostals concerned about sharing the gospel without having any interest in the reality of oppression, discrimination, and marginalization? Are they just dancing and singing in their temples, having ecstasies and emotional experiences while ignoring the poor and their social struggle? Many scholars see US Latina/o pentecostalism as a tradition with an anemic social ethic, one that lacks any significant interest in the needs of the poorest in society. However, new research on progressive pentecostals shows a different panorama. The theological perspectives and social ethics of a vast sector of US Latina/o churches have changed considerably since …

Exploring Early Temperament Predictors Of Maladaptive Behavior In A Group Of Elementary School Age Children With Autism Spectrum Disorder, Caren D. Rhodes-Doudna

Electronic Theses and Dissertations

The current study is an ex post facto nonexperimental design analyzing archival data collected from previous trials of a large-scale longitudinal study conducted by researchers at JFK Partners, Center of Excellence in Autism and Neurodevelopmental Disabilities in collaboration with the University of Colorado Anschutz Medical School (Principal Investigator: Susan Hepburn, Ph.D.). Specifically, the study looked at the temperament characteristics of mood, intensity, adaptability, and approach measured within early childhood and how they relate to later maladaptive behavior within a group of elementary age children diagnosed with ASD. Maladaptive behavior is a particularly salient outcome, as it impacts the child and …

Targeted-Ion Mass Spectrometry For The Identification Of Forensically Relevant Biological Fluids And Samples From Sexual Assault Evidence, Heather Erin Mckiernan

Electronic Theses and Dissertations

Forensic practitioners have long sought efficient and reliable means for identifying those samples that are best suited for successful genetic profiling. Traditional serological screening methodologies rely upon enzyme activity and antibody-based serological tests. These tests can be consumptive, laborious and costly while reliance on antibody-based serological testing can be prone to error. Positive results resulting from non-target biological fluids, the potential for cross- reactivity and non-specific binding events yield merely presumptive results. This has led forensic biologists to omit serological testing, at least in the case of sexual assault kit samples, in favor of Y-Screen assays. While these Y-Screen approaches …

"We Were Queens": Historical Loss Among Native Hawaiians: Exploring Historical Trauma-Informed Suicide Prevention, Antonia Rose Garriga Alvarez

Electronic Theses and Dissertations

Native Hawaiian people, and especially lesbian, gay, bisexual, transgender, māhū and/or queer (LGBTQM) Native Hawaiians, face health and mental health disparities that are disproportionate when compared with other racial/ethnic minorities in Hawai`i, and when compared to the United States as a whole. Native Hawaiians have the highest mortality rates for numerous biomedical diseases, including higher rates of substance abuse, diabetes, and even asthma, of any ethnic group in the state of Hawai`i (Andrade et al., 2006; Liu & Alameda, 2011). Suicide rates, in particular, have been rising since Hawai`i began collecting data in 1908 (Else & Andrade, 2008), and continue …

Development And Characterization Of An Inexpensive Single-Particle Fluorescence Spectrometer For Detection And Classification Of Pollen And Other Bioaerosols, Benjamin E. Swanson

Electronic Theses and Dissertations

Atmospheric aerosols are ubiquitous throughout the Earth’s atmosphere and can be important with respect to environmental systems and human health. Pollen particles are a class of primary biological aerosol particles (PBAPs) that cost the United States billions of dollars a year in loss of productivity and healthcare costs due to allergy and respiratory effects. Traditional methods of pollen detection rely on collection and subsequent identification by visual microscopy, yet few measurement stations exist in the United States. As such, current pollen forecasting models have relatively high prediction uncertainty, especially in regions without sampling stations. Recently, laser-induced fluorescence instrumentation has been …

Solar Forecasting And Integration In Power Systems, Mohana Alanazi

Electronic Theses and Dissertations

Renewable energy resources are becoming critical players in the electricity generation sector, primarily due to viability in combating global warming, effectiveness in reducing pollution caused by fossil fuel based generation, and diversifying energy mix to ensure energy security and sustainability. Solar energy is one of the most common types of renewable energy that has grown rapidly over the past decade and is anticipated to grow even faster in the future. Power supply from renewable resources is forecasted to surpass other types of generation in a foreseeable future. Numerous factors, including but not limited to the dropping cost of solar technology, …

An Hgis Approach To Land-Use/Land-Cover Change In The Blanice Watershed, Czech Republic, Kelly J. Measom

Electronic Theses and Dissertations

In the South Bohemian region of the Czech Republic, the landscape is distinguished by a network of long narrow fields bordered by hedgerows clustered in small groups. These unique clusters of hedgerows have been interacting with their environment, effectively mitigating erosion, since they were first established in the High Middle Ages. In this research project I used historical maps to characterize land-use and land-cover (LULC) change relating to hedgerow features in one cadastral territory in the Blanice Watershed. Using georeferenced historical maps from 1837 and 1952, and unreferenced historical maps from 1837 to 1953, I compared the historical LULC to …