Digital Commons Network^™

Foreign Policy In Media: An Examination Of The Captain America Films., Kat Hernandez

Electronic Theses and Dissertations

Popular culture and media are consumed daily by billions around the world, media which can contain meaningful and politically relevant material. The three Captain America films from the 2010s represent this phenomenon in the presentation of Captain America as a nationalist superhero with geopolitically relevant storylines. The direction and production of these films illustrate significant parallels to foreign policy choices of the United States. Through an interpretive analysis of the Captain America films: Captain America: The First Avenger, Captain America: The Winter Soldier, and Captain America: Civil War, this project seeks to analyze Captain America’s image and …

The Role Of Mmp-3 In Copper Oxide Nanoparticle-Induced Pulmonary Inflammation And Fibrosis., Yuanbao Zhang

Electronic Theses and Dissertations

Copper oxide nanoparticles (Nano-CuO) are wildly used in medical and industrial fields and our daily necessities. However, the biosafety assessment of Nano-CuO is far behind their rapid development. This study was to investigate the potential mechanisms underlying Nano-CuO-induced pulmonary inflammation and fibrosis, especially to determine whether Nano-CuO exposure could dysregulate MMP-3, an important mediator in pulmonary fibrosis, and its roles in Nano-CuO-induced pulmonary inflammation and fibrosis. Aim 1 was to investigate whether exposure to Nano-CuO caused MMP-3 dysregulation in lung epithelial cells and the role of MMP-3 in Nano-CuO-induced alteration of cell junction-associated proteins. The potential mechanisms that might be …

Hgf-Mediated C-Met Signaling In Human Corneal Epithelial Cells., Kate Tarvestad

Electronic Theses and Dissertations

Vision is often regarded as the primary sense of humans. The cornea is the main refractive tissue that permits light through to the retina, allowing a clear image. When the cornea sustains damage, it opens a pathway for infection and blindness through fibrotic processes. Healing the corneal tissue is critical for vision restoration and pain alleviation. Growth factors and their cognate receptors are currently under investigation as tools to restore proper corneal physiology. We hypothesize that manipulating the hepatocyte growth factor (HGF)/ c-Met signaling pathway is one route to promote quality corneal healing. This thesis investigates the signaling, trafficking, and …

The Role Of Generative Adversarial Networks In Bioimage Analysis And Computational Diagnostics., Ahmed Naglah

Electronic Theses and Dissertations

Computational technologies can contribute to the modeling and simulation of the biological environments and activities towards achieving better interpretations, analysis, and understanding. With the emergence of digital pathology, we can observe an increasing demand for more innovative, effective, and efficient computational models. Under the umbrella of artificial intelligence, deep learning mimics the brain’s way in learn complex relationships through data and experiences. In the field of bioimage analysis, models usually comprise discriminative approaches such as classification and segmentation tasks. In this thesis, we study how we can use generative AI models to improve bioimage analysis tasks using Generative Adversarial Networks …

The Mechanisms Of Mitochondrial Dysfunction In T Cell Aging During Chronic Viral Infection, Madison B. Schank

Electronic Theses and Dissertations

Human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infections induce a myriad of disturbances to CD4 T cell functions, including mitochondrial compromise, excessive inflammation, increased telomeric DNA damage and attrition, cellular exhaustion and senescence, and accelerated aging. In this dissertation, the mechanisms underlying metabolic failure, accelerated aging, and cellular dysfunctions were evaluated in CD4 T cells from healthy subjects (HS) treated with a telomere-targeting drug (KML001) or HCV-infected individuals or people living with HIV (PLHIV) compared to HS. We observed that KML001-induced telomere injury resulted in mitochondrial swelling and decreased mitochondrial membrane potential, cellular respiration, mitochondrial DNA (mtDNA) copy …

Establishing The Efficacy Of Non-Cellular Components Of Adipose-Derived Stromal Vascular Fraction In Promoting Angiogenesis., Daniel Benson

Electronic Theses and Dissertations

Microvascular disease is hallmarked by pathophysiological conditions such as endothelial senescence, intimal thickening which impairs vasodilation, and regression of the capillary beds causing tissue ischemia in the myocardium or in peripheral vascular networks. Adipose-derived stromal vascular fraction (SVF) has previously demonstrated the ability to revascularize tissue. Increasing evidence shows that regenerative cells elicit their therapeutic benefit by paracrine mechanisms, leaving open extracellular vesicles (EVs) as a potential crux of the cell therapy paradigm. To test this idea, three types of gelatin methacrylate hydrogels were employed: SVF gels, EV gels derived from SVF, and blank control gels, which were used in-vitro …

“It’S About The Two Selves”: Experiences In Code-Switching Between Home And Academic Environments, Travis Wolven

Electronic Theses and Dissertations

This qualitative research study is an exploration of how college students navigate code-switching between their home and academic environments. Data were collected from five participants using interview and small group methods. Through the lenses of Coordinated Management of Meaning (CMM) and Memorable Messages (MM) frameworks, the researcher explores how key MMs affect how participants coordinate and manage meaning in communications with others in their home and college environments. Findings were fourfold: 1) participants chose between following established and creating new rules when code-switching; 2) participants shared experiences and strategies regarding knowing when and how to code-switch; 3) preparing audiences for …

Voltage-Gated Potassium Channel Dependent Mechanisms Of Cardiovascular Adaptation To Chronic Exercise., Sean Raph

Electronic Theses and Dissertations

Exercise increases cardiac workload, escalating the demand for oxygen. The myocardial vasculature responds to the greater demand for oxygen by increasing blood flow to match the needs of the heart during augmented work. Increasing blood flow requires the coronary arteries dilate (a.k.a. vasodilation); this is mediated predominantly by vascular smooth muscle cell relaxation. Vasodilation is driven through inhibition of calcium influx into vascular smooth muscle cells. The prevention of calcium influx is largely mediated by efflux of potassium via potassium channels causing membrane hyperpolarization, which in turn closes voltage-dependent (or gated) calcium channels (VDCC). Changes in vascular smooth muscle cell …

Coping Strategies And Self-Compassion As Protective Factors In The Infant And Early Childhood Mental Health Workforce, Megan Wolff

Electronic Theses and Dissertations

The mental health field experiences high levels of stress, resulting in a greater risk of poor professional quality of life, likely exacerbated by the additional stress associated with COVID-19. The present study examined the effect COVID-19 stress had on the professional quality of life of the infant and early childhood mental health (IECMH) workforce and whether coping strategies and self-compassion acted as protective factors. Results indicated that higher COVID-19 stress was associated with higher burnout and secondary traumatic stress (STS) and lower compassion satisfaction (CS). The results also showed that the pathway from COVID-19 stress to burnout was moderated by …

Fossil Mammals From Hickory Tree Cave, Sullivan County, Tennessee, Alexis Rivera

Electronic Theses and Dissertations

Hickory Tree Cave, also known as Big Spring Cave, is located in the southern Appalachians and is known for fossils that are considered to be Quaternary in age. Fossil mammals were identified and assigned to the lowest taxonomical level possible. Most remains are fragmented or digested and it seems likely that various taphonomic processes are responsible for the resulting assemblage. The site lacks the extreme boreal component of Pleistocene cave faunas in the region (e.g. Baker Bluff Cave), with most reported taxa inhabiting Appalachian deciduous forest environments in North America today. While the presence of tapir (Tapirus sp.) …

Investigating The Pi3k/Akt/Atm Pathway, Telomeric Dna Damage, T Cell Death, And Crispr/Cas9-Mediated Gene Editing During Acute And Chronic Hiv Infection, Sushant Khanal

Electronic Theses and Dissertations

Human Immunodeficiency Virus (HIV) infection initiates major metabolic and cell- survival complications. Anti-retroviral therapy (ART) is the current approach to suppress active HIV replication to a level of undetected viral load, but it is not a curative approach. Newer and sophisticated gene editing technologies could indeed be a potent antiviral therapy to achieve a clinical sterilization/cure of HIV infection. Chronic HIV patients, even under a successful ART regimen, exhibit a low-grade inflammation, immune senescence, premature aging, telomeric DNA attrition, T cell apoptosis, and cellular homeostasis. In this dissertation, we investigated CD4 T cell homeostasis, degree of T cell apoptosis, an …

An Acute Sense Of Place: The Songs Of Norman Blake, Thomas Jutz

Electronic Theses and Dissertations

American flat-picking guitarist, singer and songwriter Norman Blake holds legendary status among guitar players, bluegrass, and folk musicians.

The aim of this research is to analyze the interaction of sense of place in Norman Blake’s songwriting. This research will explore the techniques Blake uses to create that acute sense of place. Elements of literary criticism, cultural geography, ethnomusicology, and sense of place studies, as well as historical background information on Northern Alabama and North Georgia will be employed to show how this particular region of Southeastern Appalachia has informed Blake’s songwriting.

The research questions that I aim to answer are …

A Functional Study Of Topological Dna Problem In Human T Cells During Chronic Viral Infection, Xindi Dang

Electronic Theses and Dissertations

T cells play an important role in adaptive immune system against viral infections, while premature aging and dysfunction of T cells induced by unrepaired DNA damages are always non-negligible snags during the long-term of fighting with chronic viral infections, such as Hepatitis B virus (HBV), Hepatitis C virus (HCV) or Human Immunodeficiency Virus (HIV) infection. In this dissertation, we investigated the role of topological DNA damage in reprogramming telomeric DNA damage responses (DDR), mitochondrial metabolisms, and T cell functions using CD4+ T cells derived from individuals with chronic viral infections or healthy subjects treated with topoisomerase inhibitors. The healthy human …

Importance Of The Microhabitat And Microclimate Conditions In The Northern Gray-Cheeked Salamander (Plethodon Montanus) Across An Elevation Gradient, Trevor Chapman

Electronic Theses and Dissertations

The southern Appalachian Mountains have among the highest salamander diversity in the world, largely due to local speciation in the family Plethodontidae. Plethodontid salamanders (i.e., lungless salamanders) are particularly sensitive to habitat climate conditions due to their reliance on cutaneous respiration, and their immediate environmental conditions (microhabitat) likely influence their dispersion and activity more than the large-scale atmospheric conditions. The Northern Gray-cheeked salamander (Plethodon montanus) is restricted to high elevations in the Appalachian Mountains. Our goal was to investigate the relationship between P. montanus and its microhabitat by examining behavioral preference for climatic conditions, characterizing the microclimate with …

Light And Temperature Entrainment Of Two Circadian-Driven Behaviors In The Flesh Fly Sarcophaga Crassipalpis, Raven Ragsdale

Electronic Theses and Dissertations

Circadian rhythms dictate the timing of both once-in-a-lifetime adult emergence (eclosion) and daily locomotor activity rhythms in the flesh fly S. crassipalpis. Light cycles are considered the primary environmental time cue (zeitgeber), but the life history of S. crassipalpis suggests that temperature cycles (thermocycles) may also play a key role. This work evaluates the efficacy of thermocycling as a zeitgeber in S. crassipalpis. We found that shifting both light and temperature cycles of sufficient amplitude affect the phasing of eclosion and locomotor activity, but result in different patterns. Additional experiments suggest greater thermocycle sensitivity during the late metamorphic …

Nitrogen-Doped And Phosphorus-Doped Epoxy-Sealed Carbon Fiber Ultramicroelectrodes As Electrochemical Sensors For Detection Of Hydrogen Peroxide, Emmanuel Peprah-Yamoah

Electronic Theses and Dissertations

Ultramicroelectrodes (UMEs) are useful as probes for evaluating electroactive species in confined spaces (e.g., inside living cells) and for measuring fast electrochemical reactions. However, UME applications often require modification of the electrode surface to improve selectivity and sensitivity towards target analytes. Previous research in our group demonstrated that a simple soft nitriding method introduces surface nitrogen (N)-containing groups on carbon fiber (CF), leading to improved electroreduction of hydrogen peroxide (H₂O₂) on CF-UMEs. However, sensitivity for H₂O₂ detection using N-CF-UMEs was low compared to that for other modified UMEs. As an alternative to N-CF-UMEs, …

Neurocognitive And Momentary Assessment Of Fear-Based Mechanisms Underlying The Transition From Partial To Full Remission In Anorexia Nervosa., Caroline Christian

Electronic Theses and Dissertations

Background. Anorexia nervosa (AN) is a serious psychiatric illness, with high mortality, chronicity, and societal costs. Many individuals with AN that are weight restored do not to fully remit from cognitive-affective symptoms (e.g., fear of weight gain) maintaining the disorder, contributing to the poor outcomes. This critical stage of AN is called partial remission. AN is characterized by high levels of fear and avoidance, which may prevent full remission; however, the role of fear-based mechanisms (i.e., fear approach; avoidance) in partially-remitted AN remains untested. Methods. In the current study (N = 55 partially-remitted AN), participants completed two behavioral paradigms …

Examining Effective And Realized Healthcare Access In The West South Central Division., Katherine Cameron

Electronic Theses and Dissertations

This study explores realized and effective access domains outlined by Andersen’s Behavioral Model of Health Services Use. Exploring other domains of access is critical to understanding how the healthcare system functions (Kirby & Yabroff, 2020). This study evaluates rural-urban and racial/ethnic differences in access to care using two underresearched domains of healthcare access. It also allows for exploring access disparities within rural communities amongst minority populations. This dissertation is divided into five chapters. Chapter One provides an overview of health disparities and inequities and a brief overview of the study. Chapter Two details a high-level history of racism and its …

Death Of A Bacterium: Exploring The Inhibition Of Staphylococcus Aureus By Burkholderia Cenocepacia., Tiffany Brandt

Electronic Theses and Dissertations

Antimicrobial resistance is a phenomenon of increasing concern as antimicrobial overuse and misuse eliminate current therapeutic options, ushering society into a post-antimicrobial era. Antibiotic discovery and synthesis efforts are urgently needed to counter the increasing burden of antimicrobial resistance. Staphylococcus aureus is a causative agent of a variety of clinical manifestations including bacteremia, endocarditis, soft tissue infection, osteomyelitis, and device-related infections. S. aureus infection presents additional treatment challenges due to its capacity for biofilm formation, which is a mode of growth that confers protection from antibiotics and physical elimination, and the emergence of antibiotic resistant strains, including methicillin-resistant S. aureus …

Bayesian Methods For Graphical Models With Neighborhood Selection., Sagnik Bhadury

Electronic Theses and Dissertations

Graphical models determine associations between variables through the notion of conditional independence. Gaussian graphical models are a widely used class of such models, where the relationships are formalized by non-null entries of the precision matrix. However, in high-dimensional cases, covariance estimates are typically unstable. Moreover, it is natural to expect only a few significant associations to be present in many realistic applications. This necessitates the injection of sparsity techniques into the estimation method. Classical frequentist methods, like GLASSO, use penalization techniques for this purpose. Fully Bayesian methods, on the contrary, are slow because they require iteratively sampling over a quadratic …

A New Direction For Public Understanding Of Science: Toward A Participant-Centered Model Of Science Engagement., Christopher Rickels

Electronic Theses and Dissertations

Engaging the public with science is not an easy task. When presented, scientific findings, public health recommendations, and other scientific information filter through the personal values, beliefs, and biases of members of the public. Science communicators must contend with these differences in order to be effective in cultivating a public understanding of science. Given the importance of scientific understanding for living well in a complex world, increasing science understanding through science engagement is imperative. The field of public engagement with science is dichotomized by a public information deficit approach and a contextualist approach. The deficit approach prizes the factual content …

Metabolic Foundations Of Exercise-Induced Cardiac Growth., Kyle Fulghum

Electronic Theses and Dissertations

Regular aerobic exercise promotes physiological cardiac growth, which is an adaptive response thought to enable the heart to meet higher physical demands. Cardiac growth involves coordination of catabolic and anabolic activities to support ATP generation, macromolecule biosynthesis, and myocyte hypertrophy. Although previous studies suggest that exercise-induced reductions in cardiac glycolysis are critical for physiological myocyte hypertrophy, it remains unclear how exercise influences the many interlinked pathways of metabolism that support adaptive remodeling of the heart. In this thesis project, we tested the general hypothesis that aerobic exercise promotes physiological cardiac growth by coordinating myocardial metabolism to promote glucose-supported anabolic pathway …

The Impact Of Pegylation On Cellular Uptake And In Vivo Biodistribution Of Gold Nanoparticle Mri Contrast Agents., Nagwa El-Baz

Electronic Theses and Dissertations

Gold nanoparticles (GNPs) have become a pivotal platform for the delivery of pharmaceutical and diagnostic agents as well as for general therapeutic purposes. Despite their potential for use in biomedicine, their interaction with serum proteins is crucial as it could change their biological profile due to the formation of a protein corona, which can affect their delivery to target tissues in the body. Grafting the GNPs with polyethylene glycol (PEG) is widely used in research to decrease opsonization of the particles by serum proteins and decrease the uptake by the reticuloendothelial system. Here in this dissertation, we have developed a …

Age-Related Differences In The Relationship Between Activity Familiarity And Well-Being., S. Kelly Shryock

Electronic Theses and Dissertations

Background and Objectives: This study explored age-related differences in the relationship between activity novelty/familiarity and well-being, conceptualized within theories including Selection, Optimization, and Compensation (SOC), Self-Determination Theory (SDT), and Socioemotional Selectivity Theory (SST). For all ages, participation in activities has been shown to enhance well-being. Known mediators of this relationship include psychological, physical, and psychosocial benefits. It is less clear what types of experiences are optimal across the lifespan. Research Design and Methods: A online cross-sectional, correlational study (N= 200) was conducted. Measures included demographic data, ratings of three self-identified recent activities, three activity choices of familiar or …

A Mixed Methods Assessment Of Menstrual Hygiene Management And School Attendance Among Schoolgirls In Edo State, Nigeria., Madeline Tomlinson

Electronic Theses and Dissertations

Menstrual hygiene in lower-middle-income countries (LMICs) is limited and has been linked to many health, educational and social effects. However, the prevalence, details, and impact of menstrual hygiene management have not been well characterized in rural and peri-urban settings across Nigeria. The overall purpose of this research was to investigate menstrual hygiene management (MHM) among adolescent schoolgirls in peri-urban areas of Edo State, Nigeria; and to determine the educational consequences associated with poor MHM. MHM was measured using previously validated questions that were adapted after a formative assessment of MHM within the Edo State community. We used a mixed methods …

Investigating The Income Academic Achievement Gap: An Exploration Of The Roles Of Mindfulness And Self-Concept Clarity In Low-Income College Students., Natalie Burke

Electronic Theses and Dissertations

The purpose of the current study was to examine whether linguistic patterns previously associated with trait mindfulness and self-concept clarity in low-income college students’ application essays are associated with academic performance and psychosocial variables. The aims of the study were as follows: 1) Explore relations between linguistic markers that have been previously associated with higher mindfulness/self-concept clarity and GPA and 2) Explore relations between linguistic markers that have been previously associated with higher mindfulness/self-concept clarity and anxiety/depression. Participants were 54 undergraduate students in the Cardinal Covenant program (a scholarship program for students from low-income families) who began college in the …

Changing Public Perception Of Dementia: The Effect And Credibility Of Three Informational Models., Diana Hedrick

Electronic Theses and Dissertations

Background: Since the 1970s, Alzheimer’s disease and dementia have been portrayed in a medicalized, symptom-focused manner to help spread awareness, improve diagnosis, and push for treatment research. In recent decades, there has been movement towards a biopsychosocial, person-centered approach that considers social and psychological factors that interact with biological diseases processes. Common biopsychosocial approaches retain an emphasis on deficits and symptoms. New research has integrated positive psychology constructs and dementia. This integration of positive psychology principles and dementia care and research has the potential to more accurately capture the experience of having dementia. Objectives: This dissertation tested how the portrayal …

Re-Engineering Cardio-Oncology Testing Using Biomimetic Heart Slice Cultures., Jessica Miller

Electronic Theses and Dissertations

28% of drug withdrawal from the market are due to unforeseen disruptions in cardiomyocyte contractility and electrophysiology. The most commonly used platforms for drug testing are in vivo animal models and in vitro cell culture models. While both have been of paramount importance for the discovery and detection of many cardiotoxicities and mechanisms of action, they lack the ability to model an intact human myocardium. This work aims to establish cardiac tissue slices, which are 300-micron thin tissue sections taken from the left ventricular myocardium, as an alternative platform for cardio-oncology studies, specifically cardiotoxicity testing. Additionally, this work aims to …

Alpha, Beta-Unsaturated Aldehydes: The Underrepresented Markers Of Disease., Saurin Sutaria

Electronic Theses and Dissertations

The peroxidation of unsaturated fatty acids is a widely recognized metabolic process that creates a complex mixture of volatile organic compounds including aldehydes. Elevated levels of reactive oxygen species in cancer cells promote random lipid peroxidation, which leads to an increase in a variety of aldehydes. Many of these volatile aldehydes are exhaled and are of interest as potential markers of disease. Chapter 1 presents a review of reported aldehydes in the exhaled breath of lung cancer patients. alpha,beta-Unsaturated aldehydes, detected primarily when derivatized during exhaled breath preconcentration, are underreported in the reviewed articles. Chapter 1 concludes with our hypothesis …

Mejorar La Raza: Measuring The Implicit Anti-Black Racial Bias Of Multiracial Individuals., Katalina Traxler

Electronic Theses and Dissertations

This dissertation sought to examine if there are implicit anti-Black racial bias differences across multiracial people who identify as Black/White compared to multiracial people who do not identify as Black/White, accounting for age, citizenship, and education level. It was also determined if age, citizenship status, and education level moderate the relationship between multiracial identity and implicit anti-Black racial bias. The theories guiding this dissertation were: Heider’s Balance Theory, Multiracial Identity Development, and White Identity Development. The data were collected from Project Implicit, a non-profit organization and international collaborative of researchers who are interested in implicit social cognition. The data analysis …