Digital Commons Network^™

Genes Involved In Nitrogen Assimilation In Ustilago Maydis., Sunita Khanal

Electronic Theses and Dissertations

U. maydis can utilize nitrate as a source of nitrogen from its environment, a process requiring a nitrate transporter to bring the nitrate inside the cell. This is followed by a two-step reduction of nitrate to ammonium. At first, nitrate reductase reduces nitrate into nitrite; in the second reaction, nitrite is reduced to ammonium by nitrite reductase. The goal of the study was to identify the nitrate transporter and nitrite reductase genes in U. maydis and characterize them based on their role in mating and virulence. The genes um03848 and um03849 were identified bioinformatically as encoding, respectively, the nitrite reductase …

Inventory Management Of The Refrigerator's Produce Bins Using Classification Algorithms And Hand Analysis., Sarah Virginia Morris

Electronic Theses and Dissertations

Tracking the inventory of one’s refrigerator has been a mission for consumers since the advent of the refrigerator. With the improvement of computer vision capabilities, automatic inventory systems are within reach. One inventory area with many potential benefits is the fresh food produce bins. The bins are a unique storage area due to their deep size. A user cannot easily see what is in the bins without opening the drawer. Produce items are also some of the quickest foods in the refrigerator to spoil, despite being temperature and humidity controlled to have the fruits and vegetables last longer. Allowing the …

Tissue-Resident Myeloid-Derived Suppressor Cells Modulate Immune Homeostasis In Healthy Adipose., Katlin Brooke Stivers

Electronic Theses and Dissertations

Our goal with this study was to gain insight into the homeostatic immune cell network in healthy adipose tissue. Specifically, we sought to determine if the immature myeloid cells found in healthy and inflamed adipose were, in fact, tissue-resident myeloid-derived suppressor cells (MDSCs). Using various in vitro and in vivo methods, we have uncovered a population of CD11b^Hi Ly6C^Hi Ly6G^- MDSCs resident in healthy adipose tissue. To the best of our knowledge this is the first time that these cells have been investigated and described in healthy adipose tissue. Systemic MDSC depletion lead to the accumulation of …

Epithelial Responses To Porphyromonas Gingivalis In The Community Context., Zackary Ray Fitzsimonds

Electronic Theses and Dissertations

Porphyromonas gingivalis, a keystone pathogen for periodontal disease, initiates a complex molecular dialogue with gingival epithelial cells, leading ultimately to disruption of host signaling pathways. Dysbiotic host responses are also thought to contribute to the initiation and progression of oral squamous cell carcinomas. Recently, the homeostatic commensal Streptococcus gordonii has been shown to antagonize P. gingivalis-induced epithelial cell signaling events in host cells, such as proliferation and migration. The aim of this study was to characterize pathways that P. gingivalis targets to disturb host signaling, with a specific focus on pathways that S. gordonii can restore to homeostatic …

The Effects Of Increasing Running Speed On Vgrf And Asymmetry, Kaela M. Hierholzer

Electronic Theses and Dissertations

Biomechanical and physiological parameters related to running performance are usually studied separately. However, evaluating both aspects together could be beneficial in improving athletic performance. The purpose of this study was to observe the change in peak vGRF and asymmetry as speed increases, while observing physiological responses during a O_2maxtest. Data from athlete monitoring of 12 cross-country and triathlon athletes were analyzed. The athlete monitoring protocol included three unweighted countermovement jumps and a O_2maxtest performed by the athletes. The athletes had an average O_2maxof 53.4 ± 7.7 mL/kg/min, while their average vGRF asymmetry throughout the …

Head Start Preschool Teachers’ Perceptions Of Reggio Emilia Principles Practiced Within Their Own Setting: A Case Study, Tara Terry Voit

Electronic Theses and Dissertations

The Reggio Emilia Approach (REA) and Head Start (HS) value high-quality early education, support for families, and community partnerships according to the literature. Exploring principles of REA in relation to the HS program model may reveal alignment that will inform high-quality developmentally appropriate practices that are meaningful and enhance learning outcomes. This multiple case study explores the REA in alignment within three Head Start programs in New Mexico, Massachusetts, and Tennessee. Participant groups include one education director, one education leader, and four teachers in each of the three locations. In New Mexico, the HS program was located on a Native …

The Economics Of Love, Débora Silva Viana

Electronic Theses and Dissertations

In this thesis I argue that the obligation to love based on the Christian tradition requires Christians to disperse their resources in a way that significantly evens out wealth distribution and increases social justice. Christians disagree on the terminology and some tenets of the goal of the Christian life (e.g., deification, beatific vision, communion with God, salvation). However, the requirement to practice love is common to all of these concepts, thus making love normative for Christians. I argue that when love takes such a prominent role in one’s life, then it naturally influences how one manages one’s resources. If love …

Food Security As A Basic Right For College Students: A Descriptive Study Of The Factors Associated With Food Insecurity And Higher Education Attainment, Shannon Beatrice Que

Electronic Theses and Dissertations

Food insecurity amongst college students is a rising crisis. Students’ inability to access nutritional food on a consistent basis can negatively impact student retention, academic success, mental and physical health, and social mobility, all of which are at an even greater risk due to the rise of the COVID-19 pandemic. Additionally, children and adolescents in primary and secondary school who experience food insecurity can experience developmental delays, which can impede their ability to obtain a postsecondary degree. Limited research has been conducted on the topic of food insecurity at private universities as well as identifying chronic food insecurity. The present …

Medical Noncompliance In A Pediatric Patient Living In A Single-Parent Household, Lucy Obianuju Norrell

Electronic Theses and Dissertations

Abstract Noncompliance is a patient’s inability to comply with the recommended treatment for their complete recovery from an ailment, while compliance is the extent to which a patient adheres to the provider’s directive, such as medication and orders given. The purpose of this study was to determine if motivational interviewing when applied to noncompliant pediatric patients from single-parent households, would improve global medical compliance in this population as compared to the standard of care at the end of three months. Thirty-two participants were recruited using the homogeneous purposeful sampling. The project employed a mixed-method approach. Fred Kleinsinger’s noncompliant behavior tool …

Cystic Fibrosis Transmembrane Conductance Regulator Modulator Therapies In Cystic Fibrosis: A Retrospective Evaluation Of A Nationwide Specialty Pharmacy Database, Zumi Mehta

Electronic Theses and Dissertations

Background: Cystic fibrosis (CF) is a progressive, genetic disorder caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Patients with CF experience excessive mucus build up and infections leading to complications in the pancreas, lungs, and other organs. There has been a paradigm shift in the treatment of CF with the introduction of CFTR modulator therapies and given their high acquisition cost, assessing their economic burden along with the adherence rate of patients is imperative. Objective: (i) To assess the utilization of CFTR modulator therapies in a specialty pharmacy CF patient population and (ii) to calculate …

The Effects Of Different Amino Acid Concentrations, With Or Without Chromium Supplementation, On The Performance And Yield Of Commercial Broiler Chickens Fed Two Different Feeding Programs., Ian Brock

Electronic Theses and Dissertations

Abstract

The objective of this study was to evaluate performance parameters and meat yield of commercial broiler chickens fed diets with different amino acid concentrations, with or without chromium supplementation, and delivered through two different feeding programs over 54 days. The experimental study was completed as a randomized-block design with 4,800, Ross 708 X Ross 708 commercial broiler chickens, picked at random, and evenly divided into six treatment groups (800 birds per group). Treatments 1 and 4 where placed under feeding program (FP1). Treatments 2, 3, 5, and 6 where placed under a different feeding program (FP2). Three different feed …

Exploring The Relationship Between Dietary Self-Monitoring With A Mobile App And Weight Change, Jason Payne

Electronic Theses and Dissertations

Background: Approximately 70% of the adult population in the United States has overweight or obesity and increased risk for developing chronic diseases. Dietary self-monitoring adherence using a paper diary is associated with weight loss; however, few studies have examined this association with dietary self-monitoring apps. Objectives: 1) explore if an association exists between app-based dietary self-monitoring and weight change among adults with overweight or obesity and 2) explore the relationships between the frequency, consistency, and completeness of self-monitoring and weight change. Methods: Participants self-monitored dietary intake for 8 weeks using the app Calorie Counter by FatSecret. Participants were assigned …

The Physiology And Evolution Of Selenite Respiration In Bacteria, Michael Wells

Electronic Theses and Dissertations

The selenium oxyanions selenate (Se(VI)) and selenite (Se(IV)) can be utilized by some bacteria and archaea as terminal electron acceptors in anaerobic respiration. Se(VI) and Se(IV) respiration is mediated by a phylogenetically and ecologically diverse array of organisms, suggesting that selenium respiration is ubiquitous in natural environments. Several respiratory Se(VI) reductases have been characterized in bacteria, revealing that Se(VI) respiration has evolved independently several times in this domain. Se(IV) respiration, in contrast, has yet to be characterized. I have purified and characterized the first respiratory Se(IV) reductase from Bacillus selenitireducens MLS10. The Se(IV) reductase appeared to purify as a single …

Risk Factors Associated With Pressure Injury In Pediatric Congenital Heart Disease Patients, Ashlee Shields

Electronic Theses and Dissertations

Pediatric congenital heart disease (CHD) patients have unique risk factors associated with the pathophysiology of abnormal heart function. This vulnerable population is likely at an increased risk of acquiring a pressure injury during hospitalization. There are limited studies that include congenital heart disease patients and more specifically, factors unique to these patients. The purpose of this study was to identify risk factors associated with development of pressure injury in pediatric CHD patients. This retrospective study used a convenience sample from hospital-acquired data including subjects with congenital heart disease. The results demonstrated an association between pressure injury development and variables both …

Gene Delivery To The Blood-Brain Barrier For Ischemic Stroke Therapy, Wanzhu Zhao

Electronic Theses and Dissertations

Stroke is a major cause of death in the United States. We aim to develop safe and efficient approaches for the treatment of stroke. Regenerative therapies have been identified as the major need for curing stroke [1]. We explored two different carriers (poly (ethylene glycol)_5k-poly (L-aspartate-diethylenetriamine)₄₈ (PEG-DET) / Pluronic P84 (P84) and exosomes (EXOs) / microvesicles (MVs)) to deliver brain-derived neurotrophic factor (BDNF) plasmid DNA (pDNA), a functional gene encoding a neurotrophin. In the first project, our data showed that DNA nanoparticles (NPs) with 0.03% P84 was a promising formulation to deliver Luciferase pDNA into immortalized human …

Effects Of Warming On Decomposition Of Submerged Plant Litter And Associated Microorganisms In Streamside Channels, Kaity Ackerman

Electronic Theses and Dissertations

Surface air temperatures are predicted to increase in the near future, which will likely affect microbial activity and carbon flow in stream ecosystems. I performed an experiment in streamside channels at Coweeta Hydrological Laboratory, NC to assess responses of litter-associated microorganisms to moderate increases in water temperature (5 levels, ambient to +4°C). The objectives of the experiment were to determine: (1) if there are differences in the magnitude of responses to temperature among various microbial parameters and (2) whether microbial responses to temperature vary among plant litter of different carbon quality. Thus, I measured litter decomposition rate, fungal biomass (ergosterol), …

Soil Nutrient And Contaminant Assessment In Community Gardens, Louisville, Kentucky., Jessica Lynne Eggleston

Electronic Theses and Dissertations

Urban population impact on environmental warming has inspired interest in vegetative coverage to mitigate human influence. Community gardens are proposed to increase green space and access to fresh food, improve health, and quality of life. The potential for soil contamination in post-industrial cities is high presenting a health risk for gardeners. Three Louisville, Kentucky community gardens were chosen for analysis of soil texture, pH, concentration of trace nutrients and metals using Mehlich 1 Extraction. In two community gardens, relative absence of heavy metals suggest they are currently safe for gardening. One garden had a concentration of copper and zinc over …

Factors Of Dysphagia In The Overdose Population Post Extubation., Renee Brown

Electronic Theses and Dissertations

Post-extubation dysphagia (PED) has been studied in depth in a variety of populations including elective heart surgeries, trauma, critical illness, traumatic brain injury, and those requiring mechanical ventilation, but has not been researched in the overdose population (OD). This study aims to research factors of PED in the OD population. A retrospective study was conducted and found 24 participants who fit the inclusionary requirements. Correlation statistics were run between age and duration of intubation, age or duration of intubation and gender, and between intubation duration, age, and scores on Penetration-Aspiration Scale (P/A) (thin by cup and thin by straw). The …

Good Grief: An Analysis Of The Character Development Of Tonya In August Wilson’S King Hedley Ii Through The Lens Of “The Five Stages Of Grief”., Kala Ross

Electronic Theses and Dissertations

An actor who is portraying a grieving character can also grieve simultaneously about the character’s given circumstances, which affects the character development and mental health of the actor. This thesis assesses how the character Tonya, in August Wilson’s King Hedley II, engages with grief as an African American woman and offers a process for the actor to engage and disengage with loss. “Good Grief” will unpack Constantin Stanislavski’s system of acting, focusing on the Magic “If” and the given circumstances, while also introducing the “Five Stages of Grief” coined by Elisabeth Kubler-Ross. Through the scholarship of theatre practitioners and psychologists …

Role Of Plant Volatile Organic Compounds (Vocs) In Seed Priming And Plant-Herbivore Interactions., Abhinav Kumar Maurya

Electronic Theses and Dissertations

This dissertation explores and expands the existing knowledge on the role of plant volatiles in facilitating seed priming and providing direct defense against herbivore. Although the roles of plant volatiles in priming a plant’s defenses and providing direct defense against pests is well known, information regarding their effects on seed and the fitness of future plants and direct toxicity to herbivores is understudied. This dissertation does a thorough examination of these two understudied aspects of plant volatiles and provides novel insight into the role of plant volatiles in seed priming and direct defense (Chapter I). Seeds in the soil can …

Dual Role Of Antimicrobial Peptide Cathelicidin In Alcohol-Associated Liver Disease., Fengyuan Li

Electronic Theses and Dissertations

Patients with alcohol-associated liver disease (ALD) have high morbidity and mortality in its severe forms. One of the major features in different forms of ALD is the altered gut microbiota. Antimicrobial peptides (AMPs) play a crucial role in maintaining gut microbiota homeostasis. LL-37 (known as CRAMP in mouse) is the sole member of the human cathelicidin family and has piqued great research interest for its dual role in modulating microbiota and the immune response in metabolic diseases. Inflammasome activation is an important component of the liver pathophysiology in ALD and requires two signals for its full activation to induce the …

Identifying Protective Factors Against Overweight And Obesity Within The Social Environment Of Women With Low Incomes., Monica M. Adams

Electronic Theses and Dissertations

Over two-thirds of the United States population have overweight or obese (OW/OB) weight statuses due in large part to an obesogenic environment that encourages unhealthful weight related behaviors. The obesogenic environment appears to place a larger burden on women with low incomes as they experience OW/OB disproportionately compared to other groups. Studies seeking to understand the impact of the obesogenic environment on this population have been deficit focused, largely examining environmental risk factors for OW/OB and ignoring protective factors against it. Most women with low incomes do not have an obese weight status and some women who have OW/OB statuses …

Modulation Of Host Innate Immune Cells By Yersinia Pestis To Create A Permissive Environment For Replication., Amanda Rose Pulsifer

Electronic Theses and Dissertations

Yersinia pestis has gained widespread infamy due to the historic outbreak during the middle ages, referred to as The Black Death. Infection with Y. pestis typically begins with deposition of Y. pestis into the dermis (bubonic plague) or respiratory tract (pneumonic plague). Tissue resident macrophages are the first innate immune cell encountered by Y. pestis. Macrophages are likely a way for Y. pestis to avoid neutrophils early in infection when the neutrophil neutralizing Type Three Secretion System is not expressed. This work focuses on which Rab host proteins are manipulated by Y. pestis, and how neutrophils are forced to …

The Emerging Oral Pathogen, Filifactor Alocis, Disrupts Neutrophil Functions To Enhance Survival And Dysregulate Inflammation., Irina Miralda Molina

Electronic Theses and Dissertations

Periodontitis is an irreversible, chronic inflammatory disease where pathogenic microbial communities accumulate in the gingival crevice. Advances in culture-independent techniques have facilitated the identification of new bacterial species in periodontal lesions, such as the Gram-positive anaerobe, Filifactor alocis. Neutrophils are a major component of the innate host response, and the outcome of their interaction with F. alocis may be a determinant of oral health status. While neutrophil functions typically protect the host against periodontal disease, oral pathogens have adapted to evade or disarm neutrophil microbicidal functions while promoting mechanisms that drive inflammation, which also provides a source of nutrients …

Aboveground-Belowground Interactions: Roles Of Soil Biotic And Abiotic Factors On Switchgrass's (Panicum Virgatum) Growth, Defense Against Herbivory And Cell Wall Chemistry., Binod Basyal

Electronic Theses and Dissertations

Plants constantly interact with their biotic and abiotic soil environments. Most terrestrial plants form beneficial associations with soil microbes such as arbuscular mycorrhizal (AM) fungi, which are widely known for their ability to transfer soil phosphorus and nitrogen to the host plants. They help plants tolerate drought stress and improve plant defense against herbivores such as plant-parasitic nematodes (PPNs). This dissertation investigates the role of AM fungi on switchgrass’s (Panicum virgatum) growth, cell wall chemistry, and defense against PPNs using a combination of growth chamber and field studies. Switchgrass is a native warm-season species which is gaining traction as candidate …

Evaluation Of Metal Concentration In Water And Soil In The Southern Region Of Lake Nacogdoches, Olawunmi Taiwo

Electronic Theses and Dissertations

The concentrations of metals (Ba, Cd, Co, Cr, Cu, Mn, Ni, Pb, Sr, V, Zn) were investigated in soil and water samples collected over four different sites in the southern part of Lake Nacogdoches over one year from August 2018 through June 2019. Water samples were analyzed using the USEPA method 200.8 part 11.2 for total recoverable metals. Soil samples were analyzed using the USEPA method 3050B for total recoverable metals. Although the total concentration gives some indication of the level of contamination, it is not enough to give information on the bioavailability or mobility of the element. A modified …

Functionalizing Nanoparticles With Cafa Protein To Target Bar Peptide For Oral Delivery Applications., Hetal Desai

Electronic Theses and Dissertations

Background: Porphyromonas gingivalis adherence to Streptococcus gordonii may be important for P. gingivalis colonization in the oral cavity. Nanoparticles encapsulating synthetic peptide BAR (BAR-NPs) inhibit P. gingivalis adherence more potently than free BAR. However, BAR-NPs would exhibit low retention in an open flow environment. Hypothesis: Targeting BAR-NPs to the streptococcal surface using CafA protein will enhance their efficacy. Methods: CafA-modified NPs encapsulating BAR were synthesized using double emulsion approach. Surface binding and retention, and release kinetics of BAR from CafA-modified NPs was assessed. Functional inhibition assays were performed using dual a species biofilm. Results: CafA-modified NPs demonstrated specificity of adhesion, …

Geographic Imaginaries Of Urban Spatial Segregation: A Case Study Of The West End Neighborhoods In Louisville, Kentucky., Amber Dock

Electronic Theses and Dissertations

The objective of this thesis is to translate the framework of geographic imaginaries into an urban context in order to capture a narrative of how residents conceptualize and experience segregation. This framework is rooted in an investigation of local discourses as they exist within a specific social, political, and historical context. Institutionalized segregation and structural racism are the foundations on which the American urban context studied here was built upon. This study employs multiple methods, including contextualizing the study area, analyzing discursive content, and visualizing the results. The results of these analyses included empirically connecting concentrations of protected classes to …

Evaluation Of A Jail Reentry Program., Bailey Elisabeth Holland

Electronic Theses and Dissertations

Previous research has focused on prison reentry programs and the impact the program has on reducing rates of recidivism, but there is a lack of research on jail reentry programs and the impact of programs’ individual services have on recidivism. Secondary data was obtained from the Louisville Metro Jail Familiar Faces Action and Community Transition (F2ACT) reentry program, which included basic demographic data of the participants, a record of individual services received by each participant, and the number of times each participant was booked into Louisville Metro Corrections before and after participating in F2ACT. A multinomial logistic regression found that …

Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander

Electronic Theses and Dissertations

Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …