Digital Commons Network^™

Isolation Of Novel Myxobacteria And Progress Towards Heterologous Expression Of Uncharacterized Secondary Metabolites, Andrew Ahearne

Electronic Theses and Dissertations

Myxobacteria, first observed by Roland Thaxter in the late 19th century, are known for their unique swarming behavior, complex fruiting body formation and biosynthetic potential. Myxobacteria are noted for their large genomes and the presence of numerous biosynthetic gene clusters (BGCs) that hint at their potential to produce novel metabolites with therapeutic properties. Isolation of myxobacteria utilizing E. coli and filter paper baiting yielded 20 myxobacteria isolated from rhizospheric soil samples in North America. Among these isolates, nine were identified as novel species, representing the genera Archangium, Myxococcus, Nannocystis, Polyangium, Pyxidicoccus, Sorangium, and Stigmatella. Comprehensive growth profiles, biochemical assays, and …

Efficient Design Of Neural Network Hardware Accelerator For Enhanced Performance In Deep Learning Systems, Tamador Nadem Mohaidat

Electronic Theses and Dissertations

Artificial intelligence (AI) hardware accelerator is an emerging research for several applications and domains. The hardware accelerator aims to provide high computational speed while retaining low cost and high learning performance. The main challenge is to design complex machine learning models on hardware with high performance. In this thesis, the aim is to develop the hardware implementation of perceptron within a neural network. Innovative hardware-based approaches are explored to optimize perceptron networks, enhancing accuracy, reliability, and fault tolerance while minimizing resource requirements and power consumption.

A thorough investigation into machine learning accelerators and associated challenges was initially conducted. The hardware …

Tanzanian Cyberscape: Exploring Language Preference Patterns Among Instagram Users, Carolyne Saria Ulomy

Electronic Theses and Dissertations

Virtual platforms such as Instagram are not only characterized by massive collaboration, sharing, and access to information but also by multilingual exchanges. For example, Tanzanian social media users choose to disseminate or publish information online using different languages present in their linguistic repertoires. This study explores multilingualism in social media (Instagram) within the Tanzanian context, as well as address the statuses of Swahili, English, Swanglish/Kiswanglish, Community Languages (CLs) and Foreign Languages (FLs) on social media. Specifically, the research investigated two overarching questions: What are the factors influencing language choices among Tanzanian Instagram users? and is there an association between content …

Nutrient Intake, Performance, And Body Composition In Preseason Wrestlers, Gregory Coapstick

Electronic Theses and Dissertations

BACKGROUND: College athletes, especially in weight class sports, often experience energy deficits. Weight class sports such as wrestling are at greatest risk for deficiencies, and little is known about the relationships between body composition, nutrient intake, and performance in these athletes.
OBJECTIVES: The purposes of this study were to quantify macronutrient and micronutrient intake of pre-season male collegiate wrestlers and compare to recommendations, and examine relationships among nutritional intakes, body composition, and performance measurements of strength, anaerobic, and aerobic capacity.
METHODS: Male Division I wrestlers (n=11, mean ± SD age: 21.3±1.7 years, wrestling experience: 14.9±2.5 years) were recruited during preseason. …

Influence Of Reduced Salt In Fully Cooked Ham And Sausage Cured With Natural Celery Powder On The Growth Of Listeria Monocytogenes At 4⁰C, Anne Sherod

Electronic Theses and Dissertations

This research evaluated the influence of reducing salt on outgrowth of L. monocytogenes on ready to eat (RTE) deli-style sliced ham cured with natural celery powder, fully cooked sausage links cured with traditional sodium nitrite and sausage links cured with natural celery powder, vacuum packaged and stored at 4⁰C on the outgrowth of L. monocytogenes. Experiment 1 evaluated L. monocytogenes outgrowth on ham over 153 days on the following treatments, current retail ham (HC) 2.0% salt; reduced salt 1 (HRS1) 1.5% salt; reduced salt 2 (HSR2) 1.35% salt; and reduced salt 2 without antimicrobials (HRS2WA) 1.35% salt without potassium lactate …

Various Surfactant Soil Conditioner Effect On Plant Nutrient Concentration, Soil Nutrient Depletion, Soil Volumetric Water Content, Plant Biomass And Yield In Corn, Timothy Veldkamp

Electronic Theses and Dissertations

Soil conditioners help improve soil structure by increasing aeration, water infiltration and water holding capacity leading to nutrient availability and reduced irrigation needs. The goal of this study was to evaluate the impact of multiple soil conditioners (SC1, SC2, SC3, SC4, and SC5) on corn (Zea mays L.) and their ability to reduce irrigation by 30%. This was evaluated in the field in 2022 and 2023 by Aurora, SD using a Randomized Complete Block Design with six replications. Conditioners were applied as broadcast pre-emergence treatments with 70% irrigation. Control treatments of 70% and 100% irrigation, respectively were included. SC1 application …

Impact Of Varying Molecular Weights Of Hyaluronic Acid On The Functional Properties Of Process Cheese Product, Rutvi Joshi

Electronic Theses and Dissertations

Hyaluronic acid (HA) is being accepted for use as a food ingredient and/or food supplement. It is available in a wide range of molecular weights (MW) and represents unique moisture retention properties depending on the MW. The moisture retention property of HA would be of great economic potential for the dairy industry. HA is a natural polysaccharide and holds the potential as a functional ingredient in dairy and food applications to modify viscosity and textural properties. The first research objective of the study was to determine the impact of very low to high MW of HA - 8kDa, 320kDa, 980kDa, …

Role Of Production Systems On Carcass And Palatability Attributes Of Bison, Lydia Margaret Hite O'Sullivan

Electronic Theses and Dissertations

The overall objective of this dissertation was to determine the impact of finishing systems on carcass and palatability attributes of bison. The specific objectives were 1) determine the influence of finishing system (grain-finished vs. grass-finished) on the mechanisms of tenderness formation in the longissimus lumborum of bison bulls, 2) determine the influence of diverse finishing systems on carcass characteristics and proximate composition of bison heifers, and 3) determine the influence of diverse finishing systems on palatability of striploin steaks from bison heifers. Results indicate that finishing systems differentially influence carcass characteristics, tenderness, and flavor attributes of bison. Specifically, this is …

Impacts Of Starter Fertilizer On Corn Development, Nutrient Uptake And Grain Yield In Eastern South Dakota, Larousse Dorissant

Electronic Theses and Dissertations

Cold and wet spring conditions during planting in the Northern Corn Belt can limit nutrient mobility, especially phosphorus (P) due to reduced mineralization. This often triggers the use of starter fertilizers to enhance nutrient availability within the root zone, and as a result improve nutrient uptake and the overall plant growth. Albeit research has shown that starter fertilizer applications can significantly boost corn grain yield potential, the most substantial yield responses from starter fertilization are typically observed in fields where soil test P levels fall below the critical soil test P threshold. This dissertation explores i) the effects of starter …

Investigation Of Evolution In Fungal Traits At Multiple Genetic Scales, Brooke Allen

Electronic Theses and Dissertations

The study of fungal traits is critical for understanding community assembly and ecosystem function, as traits related to resource acquisition, enemy avoidance, and stress tolerance link fungi to their ecological roles. Symbiotic relationships, such as those between ectomycorrhizal (EcM) fungi and their host plants, greatly influence fungal traits and play a key role in fungal evolution. In this study, we addressed the evolution of EcM fungal traits, particularly biosynthetic gene clusters (BGCs), at multiple genetic scales, including individual genes, genotypes within populations, and a broader phylogenetic perspective across the fungal kingdom. First, we surveyed the diversity of BGCs in native …

North Ms Foodrx Farm-To-Farmacy: A Home Delivery Local Food Prescription Model, Cameron De'cal Brown

Electronic Theses and Dissertations

This portfolio presents a comprehensive overview of my contributions and involvement in the Farm-to-Farmacy (FTF) program model. Farm-to-Farmacy is an extension of the FoodRx program which aims to address food insecurity in rural parts of North Mississippi. This program was inspired by the 2019 FoodRx program in the Mississippi Delta that assisted in delivering fresh produce to the doorsteps of the elderly and homebound. Farm-to-Farmacy incorporates a unique approach by soliciting the support of local farmers which assists in sustainability for a program of this stature but also supports community engagement and involvement. The Farm-to-Farmacy program relies on local farmers …

Advancing Diversity, Equity, And Inclusion: A Graduate Portfolio On Lebonheur Children's Hospital Initiatives, James Lee Hawkins

Electronic Theses and Dissertations

In every facet of life, diversity finds a way to play a role. By advocating for, acknowledging, and drawing attention to the disparities that emerge within the various spaces people may encounter, we consciously strive to bridge the gap, aiming for a future that is more equitable and inclusive. Diversity, equity, and inclusion (DEI) have not only become personal moral imperatives but also essential pillars at the forefront for companies, healthcare, educational institutions, and businesses alike.

Mental Health In Rural Mississippi Counties: Experiences And Beliefs Of African American Pastors, Angela R. Adams

Electronic Theses and Dissertations

African Americans seek formal mental health treatment services at lower rates than white Americans (Substance Abuse and Mental Health Services Administration [SAMHSA], 2024) and higher risk of mental illness exists among rural African Americans (Haynes et al., 2017). Religiousness and spirituality have emerged as widely utilized coping methods among African Americans, therefore it is important to understand pastoral perspectives on mental health. In addition to the type of services being sought, the decision to seek help plays an important role in treatment utilization. Along these lines, two theoretical frameworks were used to formulate this study, the Theory of Planned Behavior …

Nourishing Narratives: A Photovoice Exploration Of Black Maternal Nutrition In Tennessee, Carissa Bacon

Electronic Theses and Dissertations

This qualitative study explored the perceptions of facilitators, barriers, and feelings of empowerment related to achieving healthy nutrition outcomes among pregnant and postnatal African American women in Tennessee. Data in this study was gathered utilizing the qualitative Community-Based Participatory Research (CBPR) approach of photovoice and the Transdisciplinary Contextualized Resilience Framework (TCRF) to examine factors influencing nutrition across multiple levels. The study recruited 13 African American mothers who ages ranged from 24-35 years old and who lived in the Greater Knoxville, Tn area. The study utilized a survey to capture the participants’ demographic characteristics and employed a modified version of the …

Water Insecurity In K-12 Schools In The U.S.: Understanding State Laws, Drinking Water Violations, And Lived Experiences, Shannon O'Donnell Fitzgerald

Electronic Theses and Dissertations

Introduction: Access to safe, reliable drinking water is essential for children's health, development, and academic success. This study examines water insecurity in U.S. K-12 schools, focusing on state policies, county-level violations, associated outcomes, and school staff experiences.

Methods: This mixed-methods study utilized secondary data analysis, mapping, regression analyses, and primary data collection. Secondary data from state sources were used to examine the presence of state legislation and Department of Education and Department of Health policies on school drinking water across the United States, examine associations between state legislation and policies with county drinking water violations, and investigate relationships between water …

Structural And Binding Studies Of Marine Glycans With Potential Therapeutic Use, Hoda Alsayed Mohamed Ahmed

Electronic Theses and Dissertations

This dissertation presents a comprehensive analysis of various marine-derived polysaccharides, focusing on their structures and binding properties. Using nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR), distinct marine polysaccharides were structurally characterized, and their interactions with various biological targets were evaluated. The initial study explores a glucan isolated from Marcia hiantina, a bivalve mollusk, and its biological activity. Detailed structural analysis via NMR and chromatography techniques revealed a branched glucose-based polysaccharide with potent antioxidant and anti-inflammatory properties. Further studies on the red alga Botryocladia occidentalis identified three sulfated galactans in its cell wall. Through NMR, we characterized the least …

Competition Between Bradyrhizobium Strains For Soybean Nodulation, Lilia Ernestina Montanez Hernandez

Electronic Theses and Dissertations

Biological Nitrogen Fixation (BNF) has surged as a natural strategy to provide available nitrogen to crops by taking advantage of diazotrophs, microorganisms that can reduce atmospheric nitrogen to ammonia. Soybean, a major crop, greatly benefits from BNF by forming endosymbiotic relationships with rhizobacteria from the genus Bradyrhizobium, which culminates in the formation of specialized organs called nodules, where nitrogen fixation occurs. This is known as Symbiotic Nitrogen Fixation (SNF). Inoculation of soybean fields with high nitrogen fixing Bradyrhizobium strains to stimulate SNF is being used as a strategy to increase crop productivity and nitrogen content. However, in North America, SNF …

Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman

Electronic Theses and Dissertations

Various technologies and alternative ingredients are being developed to increase the nutrient value of fibrous feedstuffs, improve animal performance, and promote sustainable swine production. Fiber-degrading enzymes have been extensively studied in growing pigs, with few studies focused on gestating sows. When considering gestating sows have greater energy digestibility of fibrous feedstuffs than grower pigs, the efficacy of fiber-degrading enzymes needs to be determined for implementation in commercial sow diets. As fiber inclusion in gestation diets benefits sow performance, corn dried distiller grains with solubles (DDGS) are a candidate fiber source for gestation alternative energy ingredient. Functional fibers, such as yeast …

Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki

Electronic Theses and Dissertations

Chickpea (cicer arietinum) samples from five distinct varieties (crown, royal, orion, sierra, and frontier) were subjected to varying storage conditions to investigate the impact of temperature and relative humidity (rh) on their chemical, functional, physical properties, and vitamin b profile. over 360 days, samples were stored at two temperatures (21°c and 40°c) and three rh levels (40%, 55%, 65%), with collections made every 90 days. initial observations focused on key chemical properties including moisture, protein, fat, and total starch content. subsequently, protein and starch fractions were isolated for further analysis, employing techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) …

Effects Of Isoacids Supplementation In Lactating Cows’ Diet Varying In Forage Fiber Level On Performances, Feed Efficiency, And Milk Fatty Acids Profile, Sumon Ahmed

Electronic Theses and Dissertations

No abstract provided.

Evaluation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross

Electronic Theses and Dissertations

Two randomized complete block design experiments were conducted to: 1) examine the effects of soybean meal with or without additional soybean hulls in replacement of modified corn distillers grains plus (MDGS) solubles on growth performance efficiency of dietary net energy utilization and carcass traits responses in finishing beef steers, 2) determine if partial or complete replacement of dried distillers grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) in diets based upon high-moisture ensiled corn influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Experiment 1 was a 118-d …

Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias

Electronic Theses and Dissertations

Objective: Quantify South Dakota (SD)’s capacity to procure locally-sourced fruit and vegetables (FVs) for K-12 school meals.
Methods: Secondary analysis of 2017 SD agricultural census and school meal data. Yields were converted to edible cups of FVs using school meal guidelines provided by the U.S. Department of Agriculture. Student enrollment and meal redemptions were used to estimate the number of FV cups needed.
Results: Estimated SD FV production met minimum total K-12 cup requirements, except for total fruit (66%) and dark green vegetables (80%). Legumes supplied 77% of the total cups of vegetables produced in 2017, whereas dark green vegetables …

Increasing Soy Products Inclusion In Swine Diets To Improve Growth Performance, Health, And Pork Quality, Ashir Foreman Atoo

Electronic Theses and Dissertations

Despite its excellent nutritional profile, soybean meal (SBM) is limited in nursery pig diets due to its anti-nutritional factors (ANFs), which young pigs tolerate poorly. In the first study, strategies to mitigate these effects included supplementation with enzymes, alone or with acidifiers. A total of 240 weaned pigs (5.9 ± 0.8 kg average body weight [BW]) were assigned in randomized complete block design to five dietary treatments: low, medium, or high SBM diets, high SBM with multienzyme, and high SBM with multienzyme plus acidifier. Diets were fed in three phases: phase 1 (0–1 weeks), phase 2 (1–3 weeks), and phase …

Translocations Of North American Bison Across Large Climatic Gradients: Impacts On Thermoregulation, Body Size, And Helmintic Infection, Hayden Wolfe

Electronic Theses and Dissertations

Every year, one-eighth of all North American bison (Bison bison) are translocated, often across large climatic gradients. Over the last 16 years bison have been translocated from Wind Cave National Park in South Dakota to establish six herds across the United States central grasslands in Iowa, Missouri, Illinois, Indiana, and Kansas. Influence of climatic gradients on herd-level thermoregulation, body size, and helmintic infection were evaluated. Overall, 1174 thermal images and 1050 fecal samples were collected between 2021 and 2023. Physiological heat flux was used to measure thermoregulation, asymptotic body mass (ABM) was used for body size, and helminth genera abundance …

Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson

Electronic Theses and Dissertations

The objective of this thesis was to determine the influence of beef hot carcass weight on proteolysis to better understand early postmortem proteolysis. Beef tenderness formation is influenced by various factors, such as temperature decline and proteolysis. Limited focus has been given to the effects of carcass weight on proteolysis. This study aimed to investigate the influence of increased beef hot carcass weight (HCW) on proteolysis of four muscles (serratus ventralis, longissimus thoracis, longissimus lumborum, and semitendinosus) during early postmortem aging times. Beef steers (n =12) were harvested and categorized into two weight groups: lightweight (LW; HCW = 349 ± …

2,3,4 Trihydroxybenzoic Acid, A Plant Secondary Metabolite Inhibits Cancer Cell Growth, Mina Akter

Electronic Theses and Dissertations

Nature offers many sources such as fruits, beverage crops, vegetables, herbs, spices, tea, dark chocolate, and wine, that are rich in hydroxybenzoic acids (HBAs). They are a prominent phenolic acids with excellent biochemical and antioxidant properties. Due to the vast dietary health benefits of HBAs such as anti-inflammatory, anti-allergenic, immunoregulatory, antimicrobial, antithrombotic, antidiabetic, anticancer properties and cardioprotective capabilities, they are attracting the attention of physicians and researchers alike to understand their mechanisms of action. It is anticipated that the understanding of the role of HBAs for their greater use in diet/supplements is beneficial to reverse common illnesses such as inflammation, …

Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl

Electronic Theses and Dissertations

The Afrikaner breed of cattle is indigenous to South Africa and due to their hardiness was once the most popular breed amongst farmers. The objectives for this study were to: (1) estimate environmental effects (age at first calving, season) and (2) genetic variance affecting longevity in Afrikaner cattle using survival analysis and a Weibull model and (3) evaluate the effect of the Infusion Project on longevity utilizing a Cox model. For the first objective, age at first calving impacted longevity as younger age at first calving was associated with decreased longevity. Season of birth and year of birth also impacted …

Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga

Electronic Theses and Dissertations

Weaning is a necessary step in pig production. After weaning, pigs are subjected to various nutritional, psychological, and environmental stresses. The weaning period is an important period for the intestinal development of the pig. During this time, digestion, immunity, metabolism, and various other aspects of the pig change rapidly. Therefore, providing high-quality feed ingredients is crucial to encourage the newly weaned pig to start consuming solid feed. Two independent studies were conducted to determine if the inclusion of: 1) corn fermented protein (CFP) or 2) probiotics or postbiotics impact nursery pigs' growth performance and gut health. The first experiment utilized …

The Effect Of Social Determinants Of Health On End-Stage Kidney Disease Mortality Across Diverse Adult Populations: Systematic Review And Meta-Analysis, Prince Agyapong

Electronic Theses and Dissertations

Background: This systematic review and meta-analysis aimed to examine the influence of social determinants of health (SDOH) on End-Stage Kidney Disease (ESKD) mortality among diverse racial populations. Given the high morbidity and mortality associated with ESKD, understanding the impact of various SDOH factors across different racial groups is crucial for improving patient outcomes.
Methods: A comprehensive literature search was conducted to identify studies reporting on the relationship between SDOH and ESKD mortality using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) format. Citations were collated in EndNote 21 and screened in Covidence by two independent reviewers, with inter-rater …

The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter

Electronic Theses and Dissertations

Phosphorus (P) management is an ongoing challenge in modern agriculture. Due to the importance of P in agriculture, P fertilizer additions are made throughout the U.S. to avoid nutrient-depleted soil. However, the application of P is problematic, as an excess of P in an agricultural system elevates the risk of environmental degradation, and current synthetic P sources are a fleeting resource. Additionally, a majority of P found in agricultural soils are insoluble, in the form of secondary and primary P minerals, making this large pool of P unavailable to plants. Fortunately, soil fungi organisms, such as arbuscular mycorrhizal fungi (AMF) …