Developing Solvent Tolerant Microbial Membranes: Lipid Extraction And Laurdan Fluorescence Approaches For Biofuel Optimization, Gladstone Anku

Electronic Theses and Dissertations

The high demand for sustainable energy has increased interest in advanced biofuels, though production is limited by solvent toxicity to microbial hosts. This study investigated the role of lipid composition in modulating membrane fluidity and solvent tolerance using Bacillus subtilis as a model organism. Five strains were cultured, and membrane lipids were extracted and analyzed using thin-layer chromatography and gas chromatography–mass spectrometry; however, inconsistent results limited compositional characterization. To address this problem, reconstituted vesicles composed of phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) were used as model membranes. Membrane fluidity was assessed using Laurdan fluorescence spectroscopy by measuring generalized polarization (GP) values …

Characterization Of Catechol-Type Siderophore(S) From P. Mosselii Cfml 90-83, Kemper R. Rasnake

Electronic Theses and Dissertations

Iron is essential for many biological processes, where it is used as a cofactor. Even though it is abundant in the Earth’s crust, it is in an insoluble form unavailable to microorganisms in the soil. To overcome this, bacteria produce siderophores, which are secondary metabolites that have a high affinity for iron. This allows bacterial cells to obtain insoluble iron that is essential for survival. Pseudomonas mosselii, a Gram negative bacterium, was isolated from soil. It was selected for further analysis as it was found to produce siderophore(s) under iron-limited conditions, which have not been characterized previously. Therefore, we aimed …

Health Professions Students’ Perceptions Of Medical Laboratory Scientists: Professional Practice And Scope Of Practice, Thomas W. Williamson

Electronic Theses and Dissertations

Medical Laboratory Scientists (MLSs) are vital yet often overlooked members of the healthcare team. This study investigated perceptions of MLSs among students in medicine, pharmacy, and nursing programs. Grounded in professional identity theory and literature on interprofessional stereotypes, the study utilized a quantitative survey distributed to 1,113 students, with 71 responses analyzed. Nine research questions explored differences in perceptions of MLSs’ professional practice, scope of practice, entry requirements, value as team members, and consultative roles. Results indicate students generally value MLSs as members of the healthcare team and acknowledge their contributions to patient care; however, their understanding is frequently restricted …

Staying The Course: Community College Student Lived Experiences On Program Completion And Motivation, Jessica Leigh Mills

Electronic Theses and Dissertations

The purpose of this phenomenological study was to explore the lived experiences of former community college students who successfully completed an associate degree, certificate, or program of study and to understand how they experienced the personal, institutional, and environmental factors that supported their persistence to completion. The study, guided primarily by Tinto’s (1975,1988) Model of Student Departure and was complemented by Bandura’s (1988) Social Learning Theory (SLT), and Maslow’s (1943) Hierarchy of Needs, explored the extrinsic and intrinsic motivations students attributed to their successful completion of their associate degree, certificate, or program of study. Twelve former community college students who …

Teacher Autonomy In Accountability-Driven Contexts: A Phenomenological Study Of Middle School Mathematics Teachers In East Tennessee, Hollyann Perdue

Electronic Theses and Dissertations

The purpose of this qualitative phenomenological study was to explore East Tennessee middle school mathematics teachers’ experiences of professional autonomy and how those experiences relate to institutional decision making within the accountability-driven educational environment. This study utilized the Self-Determination Theory (Ryan & Deci, 2017) as a framework to examine how teachers perceived their ability to make instructional decisions while navigating district expectations, pacing requirements, mandated curriculum, and state testing pressures. Data were collected through one-on-one semi-structured Zoom interviews with 12 East Tennessee middle school mathematics teachers representing varying levels of teaching experience, effectiveness ratings, and instructional settings, including both general …

Weathering More Than Storms: Understanding The Mental Health Disparities Of Black Farmers In The Appalachian Region, Aliyah Smith

Electronic Theses and Dissertations

Black farmers in Appalachia represent a vital and historically marginalized population within one of America’s most economically distressed regions. Black farmers have endured generations of systemic racism, resulting in land loss, economic hardship, and mental stressors. This study examines the intersection of race, agriculture, and behavioral health to illustrate how structural barriers and cultural stigmas shape the mental well-being of Black farmers across Appalachia.

Findings from the analysis will guide the development of a conceptual model based on constructs from the Social-Ecological Model (SEM), Intersectionality Theory, and Critical Race Theory (CRT). This study will use Key Informant Interviews (KIIs) conducted …

An Exploration Of The Psychological Impacts Of Code-Switching, Cristina Culler

Electronic Theses and Dissertations

This dissertation is an interpretative phenomenological analysis of the lived experiences of four Black American adults who have frequently engaged in code-switching. Historically, code-switching has been perceived as a necessary skill that helps marginalized folx adopt the norms and practices of the dominant culture. As a result, code-switching has become a routine practice specifically for Black Americans who often occupy white spaces. Since code-switching has been perceived as a skill, the oppressive origins of code-switching often go unnoticed. Therefore, this dissertation examines code-switching through a critical lens that deems this practice a product of racist ideologies that prioritize whiteness. Through …

Navigating The Dark Side: Emotional Labor's Impact On Hr Professionals' Wellness, Esmeralda Amelia Rendón

Electronic Theses and Dissertations

This qualitative descriptive study was designed to document and describe how female-identifying human resource (HR) generalist practitioners serving as sole or primary employee relations practitioners in small- to medium-sized organizations across the United States experience and manage emotional labor in their roles, cope with its demands, and how these experiences impact their wellness and perceptions of organizational support. Despite the centrality of emotional labor (EL) in human resource professionals’ (HRPs) roles, little was known about how HRPs in these underresourced environments cope with these demands. Data were collected through semistructured virtual interviews with eight participants and analyzed using Braun and …

Forest Cover Effects On Canopy Interception Loss, Evapotranspiration, And Soil Infiltration On Stormwater Detention Basins, Alexandria N. C. Perez

Electronic Theses and Dissertations

Developing cities and increased impervious surfaces has resulted in more surface runoff and severe flooding. Harris County, Texas has experienced major flooding events, such as Hurricane Harvey in 2017 which was estimated to be a 9,000-year flood event. The Harris County Flood Control District (HCFCD) is dedicated to creating, maintaining, and finding new ways to reduce flooding damage such as the use of detention basins as green infrastructure. These basins are either populated by grass or are planted with various tree species. This study will identify the planted trees’ canopy interception loss, reference evapotranspiration, and enhanced soil infiltration effects within …

The Impact Of Student Behavioral Demands On Teacher Burnout In Secondary Education, Adrienne J. Smith

Electronic Theses and Dissertations

ABSTRACT

Teacher burnout is a growing concern in secondary education because it affects teacher well-being, instructional effectiveness, and retention (Anton & Ryzin, 2024; Madigan & Kim, 2021). This quantitative correlational study examined the relationship between behavioral demands and emotional exhaustion among 64 Texas secondary public-school teachers. Guided by the Job Demands–Resources (JD–R) model and Conservation of Resources (COR) theory, the study also examined whether teacher self-efficacy and school climate moderated this relationship. Behavioral demands were measured as a composite of student aggression, defiance, and inattentiveness. Pearson correlations and moderation analyses conducted in Jamovi indicated that behavioral demands were positively associated …

A Modest Proposal For Discourse Analysis In Nonbinary Communities: Method And Case Study, Nikoli Michael Daigneault

Electronic Theses and Dissertations

While linguists have long used discourse analysis to study language variation in binary and nonnormative gender groups, nonbinary people have often been excluded from analyses or treated without a nonbinary-informed framework. Nevertheless, nonbinary language use can provide key insights into the relationship between gender and language. In this thesis, I propose Nonbinary Discourse Analysis, a set of recommendations and best practices for conducting discourse analysis in nonbinary communities. These methods are informed by previous empirical research on nonnormative gender identities and theoretical work in trans linguistics (Zimman, 2020) and trans epistemologies (Nordmarken, 2019; Raclaw, in press). To demonstrate the methodology, …

Illuminating Sanja Matsuri: Lived Religion And Festival Practice In Asakusa, Caleb R. Merckle

Electronic Theses and Dissertations

This thesis examines Sanja Matsuri, a major Shintō and Buddhist festival in Asakusa, Tokyo, through the lenses of lived religion, participant observation, autoethnography, and anthropology. Departing from institution-centered approaches, it foregrounds the experiential dimensions of festival participation, showing how physical devotion, collective movement, and festive intensity function as expressions of spiritual meaning. Ethnographic fieldwork conducted in 2023 and 2025 included participant observation, informal interviews with local and foreign participants, and Reflexive Discourse Analysis of fieldnotes and bilingual transcripts. Analysis reveals a dynamic interplay between formal sacralization strategies — such as mitama-ire rituals and structured processions — and spontaneous, informal participant …

The Effectiveness Of Writing Professional Development For Instructional Leaders On Student Academic Outcomes, Kourtney Y. Lavergne

Electronic Theses and Dissertations

The purpose of this qualitative case study was to explore how elementary instructional leaders in a Southeast Texas school district perceived and utilized the R.A.C.E. (Restate, Answer, Cite, Explain) professional development training as an instructional tool to improve student writing performance. The study was guided by two research questions: (a) How do elementary instructional leaders perceive the R.A.C.E. professional development training as an effective English writing curriculum tool to improve student performance? and (b) How do instructional leaders utilize the R.A.C.E. professional development training to improve student writing performance in their classrooms? The study was grounded in adult learning theory …

A Perspective As Observed By Public Middle School Teachers On A Lack Of Empathy Demonstrated By Public Middle School Students, Gail P. Hall

Electronic Theses and Dissertations

The purpose of this qualitative descriptive study was to explore a perspective as observed by public middle school teachers on a lack of empathy demonstrated by public middle school students. The research analyzed how public middle school teachers in the southern region of Texas described their perception of how a lack of empathy demonstrated by public middle school students impacted students during the school day. The study involved 10 selected participants who participated in a semistructured open-ended interview on the Google Meet platform. The participants consisted of 90% female and 10% male, with a combined total of 91 years of …

From Hashtags To Regime Change: How Ai-Driven Social Media Algorithms Fueled Youth Protests Against Authoritarianism In Bangladesh But Stalled In Pakistan (2020-2025), Rafia Nishan Abbasi

Electronic Theses and Dissertations

In this research, it has been investigated that how and why youth protests were amplified by AI based social media algorithms in Bangladesh; however, their impacts resulted in occasional and isolated resistance in Pakistan during 2022-25, taking in account the differences in state regulations, platform governance, and civil society networks. While utilizing Mixed-methods approach, this study employs comparative discourse analysis, a structured survey (N=466), semi-structured interviews, legal and policy tracing, and platform data. This research has proposed a novel theoretical framework of Algorithm Regime Mediation (ARM). ARM considers platform algorithms as politically intermingled intermediaries which can determine socio-political outcomes by …

Phase-Resolved Measurement And Arbitrary Mode Conversion Of Structured Light, Somayeh Ghazanfarpour

Electronic Theses and Dissertations

This dissertation presents a single-shot phase and amplitude retrieval technique based on spatial light modulator (SLM)-based digital holography. The proposed method enables accurate reconstruction of complex optical fields from a single interferogram, reducing sensitivity to environmental fluctuations and improving experimental stability compared to conventional multi-shot approaches. In addition, a programmable arbitrary mode converter is developed using SLM-based wavefront shaping. By introducing controlled astigmatism and engineering the differential Gouy phase between orthogonal components, the system enables flexible transformation between structured light modes, including Hermite–Gaussian and Laguerre–Gaussian beams. Numerical results demonstrate reliable phase recovery and efficient mode conversion, validating the performance of …

Disproportionality And Possibilities: State-Level Policy Recommendations For Special Education Oversight In A Shifting Federal Landscape, Amanda B. Roberts

Electronic Theses and Dissertations

This study employed a convergent parallel mixed-methods design to explore how U.S. states identify and respond to disproportionality in special education within a shifting federal policy landscape. As uncertainty surrounds the future role of the U.S. Department of Education, questions have emerged about states’ capacity to safeguard educational equity. Without federal oversight, states would assume responsibility for maintaining protections for students with special needs, making it critical to strengthen safeguards for marginalized and underresourced populations.

This study analyzed the current special education governance structures nationwide to inform equity-oriented policy and practice recommendations. Quantitative data were collected from Individuals with Disabilities …

Otherness And The Trinity: Toward A Critical Reimagining Of Christian Divinity From A Postcolonial Ethos, Taylor Muma

Electronic Theses and Dissertations

In this dissertation, I address the Trinity’s potential as an emancipatory relational archetype in the philosophy of religion, while at the same time recognizing the complicity of Christian doctrine in colonial conquest. My dissertation will be a reimagining of trinitarian theology as an ecotonal paradigm of multiplicity and alterity that provides a generative means of moving beyond the enmeshment of divinity with constructs of mastery.

The post-structuralist and postcolonial methodologies in this project emerge from the work of such scholars as Homi Bhabha, Jacques Derrida, Emmanuel Levinas and Gloria Anzaldúa. They foreground critical ideations of otherness and multiplicity in ways …

The Organizational Experience Of Next Gen Learning Through Portrait Of A Graduate, Kathleen J. Campbell

Electronic Theses and Dissertations

This qualitative single-case study investigates the organizational journey of navigating the complexities of systemic transformation. In 2021, Silver Peak School District (SPSD; a pseudonym), a rural educational organization in the western United States, implemented a graduate competency framework known as Portrait of a Graduate. This study analyzed the experiences of teachers, instructional coaches, and principals with the explicit Portrait policies, practices, and resource flows of five learning structures—graduate competencies (in SPSD, this is analogous to Portrait), deeper learning, agency, performance assessment, and teaching culture—in a district in the messy middle of Portrait adoption. By focusing on a district situated in …

She Believed She Could, So She Did: A Lifecycle Approach To Understanding Leadership And Self-Efficacy Development In Women Astronauts, Savanah Faith Sellars Bray

Electronic Theses and Dissertations

This dissertation examines how women leaders develop and sustain self-efficacy, and develop as leaders, throughout their lives. Although decades of research document the barriers women face navigating leadership journeys, including the challenges described in the Women’s Leadership Labyrinth of prejudice, discrimination, and childcare issues, existing literature offers limited insight into how women actively build belief in their capacity to lead and accomplish their goals. Further, research consistently shows that women report lower leadership self-efficacy than men, even with comparable experience, yet the developmental processes that shape this gap, beginning in childhood, remain underexplored. Guided by self-efficacy research and its four …

A Case Study Of Principal Preparation In A Social Justice-Informed Principal Preparation Program, Katie Golieb

Electronic Theses and Dissertations

This qualitative case study examined how a social justice-informed principal preparation program fostered the development of socially just school leaders and how graduates perceived their capacity for resilience as educational leaders in the contemporary PK–12 context. The Mountain Range Principal Preparation Program (MRP3), a university–district partnership designed to prepare educational leaders to be transformative leaders equipped to improve student achievement and address challenges in low-performing schools, served as the site of inquiry. The study explored how principals made meaning of their preparation experiences and how those experiences shaped their enactment of equity-focused leadership and their ability to sustain such work. …

A Computational Micropatterning Approach To Study Caveolae And Protein Localization In Vascular Biology, Andrew B. Grespin

Electronic Theses and Dissertations

Caveolae are specialized, flask-shaped membrane invaginations highly expressed in endothelium and dysregulated in atherosclerosis. Caveolae play a central role in buffering membrane tension, yet the principles governing their spatial organization remain elusive. Thus, we sought to generate the most comprehensive and systematic analysis of blood vessel caveolar spatial organization. However, cell culturing, the backbone of human-focused biological research, does not standardly do well to model physiologically relevant cell behaviors such as migration or polarity-based tissue formation, particularly for punctate proteins and structures like caveolae. Micropatterns are cell-adhesive shapes that biophysically confine cell(s) to a user defined geometry which stereotype organelle …

Defying Impossibility: Critical Qualitative Reorientations To Black Lgbtqia+ Sense Of Belonging, Olivia H. Hunte

Electronic Theses and Dissertations

Belonging is a human need central to survival and can be cultivated within all spheres of life. Social environments are vital for experiencing a sense of belonging. In some social environments, Black LGBTQIA+ people encounter greater impediments to their daily lives with consequences for survival, including threatening their belonging needs. Within the United States, systems of oppression create and inform conditions within social environments that can potentially thwart pathways to belonging for Black LGBTQIA+ people. Thus, fulfillment of belonging needs for Black LGBTQIA+ people is one of nuance, pain, resilience, hope, harm, trauma, resistance, and healing; scant research is available …

Difficult History During Difficult Times: Amache Junior Rangers, Katelyn Morkovsky

Electronic Theses and Dissertations

My research aims to understand approaches to teaching difficult histories to children at cultural heritage institutions. To do this, I examined the creation process of the Amache Junior Ranger booklet that launched at the site in the fall of 2025. Amache, a WWII Japanese American incarceration center, presents significant interpretive challenges in balancing the traumatic nature of the site with age-appropriate content as well as highlighting the agency people exercised without losing the gravity of the situation. In addition to how the booklet was made, I use my research to situate the booklet in the social and political climate in …

African American Business Owners' Descriptions Of Experiences With Lending Practices, Wannika R. Muhammad

Electronic Theses and Dissertations

Entrepreneurship has remained an influential and unfulfilled goal for African Americans from the 1800s until now. Due to barriers in lending practices, increased business retention rates for African American business owners still need to be achieved. The lack of credit and lending resources hinders business growth and retention. Although Black-owned firms contribute significantly to the U.S. economy, they remain classified as underutilized entities that benefit the least, without adequate access to financial services, such as banking and lending opportunities. This study’s participants consisted of 12–15 African American business owners in central Texas, through a qualitative descriptive exploration of their experiences …

A Quantitative Study On Broadband Access Rates In K-12 Schools And Student Academic Achievement, Tonya Michelle Todd

Electronic Theses and Dissertations

As technology evolves, so do K-12 educational platforms and policies. However, research into broadband access’s influence on student academic achievement is limited and some are outdated. Without adequate knowledge on the topic, educational and policy leaders are at risk of misallocating educational technology funds. Given the substantial monetary investment involved in the implementation of this public education policy and the limited research on the influence of broadband access rates on student academic achievement, the purpose of this study is to investigate whether the SC Educational Technology Plan’s broadband expansion influenced student academic performance.

This quantitative study sought to determine whether …

Suburban Black Girl: Lived Experiences Of Black Girls And The Impact Of Safe Spaces Within Predominantly White Institutions, Kennedi A. Stevenson

Electronic Theses and Dissertations

As Black and Biracial girls navigate the landscape of suburban Predominantly White Institutions (PWIs), they encounter a unique convergence of institutional surveillance and interpersonal erasure. While existing literature often focuses on the “achievement gap” or urban school settings, this qualitative study investigates the lived experiences of Black girlhood within the sea of Whiteness that defines suburban education. Specifically, this research explores how a gendered sanctuary— BSU Salon Talk — serves as a site of resistance and a catalyst for wellbeing. Grounded in Black Feminist Thought Theory (BFTT) and Intersectionality, the study utilizes the theoretical frameworks of Patricia Hill Collins’ Domains …

"It Was Time To Go": A Case Study In Occupational Culture, Tiffany M. Kline Costa

Electronic Theses and Dissertations

Abundant examples of negative community-police interactions demonstrate strained relations between marginalized community members and police across the United States. Likewise, nationwide data demonstrates discrepancies in how officers interact with People of Color versus White citizens. To gain insight into influences on officer behavior, this research shines a spotlight on the occupational culture of the Pittsburgh Bureau of Police (PBP) and the ways this specific occupational culture informs officer thoughts and behaviors. Via the analysis of the experiences of four former PBP officers, this research explores how culture impacts officer perspective, thoughts, and behaviors. Utilizing an interpretative phenomenological approach, data analysis …

Technology In Waldorf Education: Benefits, Challenges, And The Teacher's Role, Jennifer C. Larson

Electronic Theses and Dissertations

Historically considered a screen-free education, Waldorf pedagogy has gradually begun to integrate digital technology into the middle and upper grades over the last two decades. Research on the implications of extended screen time for children has driven this pedagogical decision alongside foundational commitments to relational practices, experiential learning, and imaginative artistry.

At the same time, recent years have marked an acceleration in digital expansion in education. COVID-19 normalized teaching and learning through remote learning platforms, and digital access is now an expected feature of daily life.  As Waldorf education seeks to offer holistic education in the context of this societal …

Becoming A "Still Point Of Grace": Identifying Obstacles Faced By Catholic Elementary School Teachers In Meeting The Needs Of Students With Generalized Anxiety In The Classroom, Kerry Smigielski

Electronic Theses and Dissertations

In the midst of a mental health crisis in the United States, teachers in the Diocese of Cityville need to be equipped to serve elementary students who experience anxiety within an authentic Catholic context. The purpose of this study is to begin improvement research on classroom supports for students with generalized anxiety in the Diocese of Cityville elementary schools. Journey lines and a focus group discussion were conducted within a causal systems analysis to respond to the central research question: “What obstacles do Catholic elementary school teachers in the Diocese of Cityville face in meeting the needs of students with …