Digital Commons Network^™

Microrobots For Wafer Scale Microfactory: Design Fabrication Integration And Control., Ruoshi Zhang

Electronic Theses and Dissertations

Future assembly technologies will involve higher automation levels, in order to satisfy increased micro scale or nano scale precision requirements. Traditionally, assembly using a top-down robotic approach has been well-studied and applied to micro-electronics and MEMS industries, but less so in nanotechnology. With the bloom of nanotechnology ever since the 1990s, newly designed products with new materials, coatings and nanoparticles are gradually entering everyone’s life, while the industry has grown into a billion-dollar volume worldwide. Traditionally, nanotechnology products are assembled using bottom-up methods, such as self-assembly, rather than with top-down robotic assembly. This is due to considerations of volume handling …

Food Insecuity In Historically Disenfranchised Parts Of Jefferson County, Ky., Kaitlyn Smith

Electronic Theses and Dissertations

The objective of this study is to investigate the availability of fresh and nutritious foods in Louisville’s West End communities. This study operates under the assumption that residents in Jefferson County have unequal access to food. This study also addresses the question of how residents and local non-profits feel about strategies that build community through food. This study is significant because across Louisville, more than 120,000 people are living with food insecurity, meaning they don't have reliable access to healthy, affordable food (Dare to Care 2019). Multiple reports that have been commissioned by local non-profits and metro departments in the …

Experimental And Numerical Study Of Process Parameters Effects Towards Part Quality And Porosity During Powder Bed Additive Manufacturing Processes., Subin Shrestha

Electronic Theses and Dissertations

Powder bed additive manufacturing (PB-AM) process utilizes an electron beam or a laser as a heat source to melt the metallic powder particles. These processes have the capability of freeform fabrication, however certain defects such as porosity, high surface roughness, etc. would hinder its application. It is important to understand the effect of the process parameters and the underlying physical phenomena, which lead to the formation of such defects. In this regard, a three-dimensional (3D) thermo-fluid model is developed to study the effect of beam speed on the surface morphology during powder bed electron beam additive fabrication (PB-EBAF). Besides, the …

A Novel Mathematical Model Of The Trojan Y-Chromosome Strategy With Optimal Control, Christopher Turner

Electronic Theses and Dissertations

Invasive species are a prevalent problem all over the world. Controlling and eradicating an invasive species is an even more diffcult problem. The Trojan Y Chromosome (TYC) eradication strategy is one control method. This method alters the female to male sex ratio by introducing sex reversed males called supermales. These sex reversed males can only produce male progeny. Mathematical models of this strategy have shown that a population can be driven to extinction with a continuous supply of these sex reversed males. There are many different mathematical models of this strategy, but most have serious flaws, such as negative solutions …

Mathematical Characterization Of A Microfluidic Ultrasound Driven Transfection Device., Chris J. Holton

Electronic Theses and Dissertations

The objective of this thesis is to develop a mathematical model characterizing the behavior of a microfluidic sonoporation device in order to understand how standing wave conditions influence molecular delivery to cells and determine whether the model predicts device performance. A prior model based on an ultrasonic separation cell that uses standing waves to separate particles is adapted for translation to the microfluidic device. This study generates data on acoustic pressure profiles across the cell as well as identifying optimal driving frequencies. This model is validated and the equations and methods for developing this model are translated to the microfluidic …

Labview Improvements And Equipment Redesign Of Unit Operations Distillation Column, Madalyn S Wead

Electronic Theses and Dissertations

Unit Operations labs are important tools to provide hands-on learning with various chemical processes. The distillation lab experiment allows students to connect equations and definitions from textbooks to the physical processes of distillation as they occur. LabVIEW was the software development tool used to create a program to control the distillation column from startup to shutdown. Improvements were made to both the distillation process equipment and the LabVIEW code to create a more interactive lab experience, enhance students’ conceptual understanding of distillation, and prepare students for their future career as chemical engineers.

Equipment addressed included non-linear valves, malfunctioning temperature and …

Functional Nanomaterials By Combining Aminooxy Chemistry And Iodine Activation At Momolayer Protected Clusters., Tirtha Raj Sibakoti

Electronic Theses and Dissertations

Aminooxy (-ONH₂) groups are most commonly known for their chemoselective reaction with carbonyl compounds (aldehydes/ketones) under mild reaction conditions. Aminooxy-based click chemistry is a versatile means of ligation as evidenced by broad application in material science, biology, biochemistry, analytical chemistry, and nanoscience. Our work exploits the facile reaction of aminooxy groups presented on the surface of gold (Au) or palladium (Pd) monolayer protected clusters (MPCs) with various aldehydes via oximation reactions, which form the robust oxime ether adducts. The functionalization of hexanethiolate-stabilized Au MPCs with a newly developed trifunctional amine-containing aminooxy alkanethiol ligand by thiol place-exchange affords aminooxylated …

Joint Location And Inventory Models And Algorithms For Deployment Of Hybrid Electric Vehicle Charging Stations., Jie Zhang

Electronic Theses and Dissertations

This thesis describes a study of a novel concept of hybrid electric vehicle charging stations in which two types of services are offered: battery swapping and fast level-3 DC charging. The battery swapping and fast-charging service are modeled by using the M/G/s/s model and the M/G/s/$\infty$ model, respectively. In particular, we focus on the operations of joint battery swapping and fast charging services, develop four joint locations and inventory models: two for the deployment of battery swapping service, two for the deployment of hybrid electric vehicle charging service. The first model for each deployment system considers a service-level constraint for …

Optimization And Simulation Models To Improve Access To Organ Transplantation In The United States., Fatemeh Karami

Electronic Theses and Dissertations

Organ allocation in the U.S. is administrated by the United Network of Organ Sharing (UNOS). UNOS’s mission is to ensure fair and equitable allocation of organs as stated in the Code of Federal Regulations, which reads "neither place of residence nor place of listing shall be a major determinant of access to transplant". Despite the regulations, there has been endless controversy surrounding the disparity in access to organ transplants. In this context, the primary research goal in this dissertation was to reduce geographic disparity in access to transplants in the U.S., with a focus on heart and kidney transplants. To …

Computational Modeling Of Photolysis And Catalysis Reactions In Vitamin B12-Dependent Enzymes., Abdullah Al Mamun

Electronic Theses and Dissertations

Vitamin B₁₂ is a complex organometallic molecule, the derivatives of which such as adenosylcobalamin (AdoCbl) and methylcobalamin (CH₃Cbl) act as a cofactor in numerous enzymatic reactions. These two biologically active cofactors contain a unique organometallic Co-C σ bond. Important feature of this Co-C bond is that it can be activated by both thermally and photolytically inside the enzymatic environment as well as in the solution. In the case of enzymatic reactions where AdoCbl molecule act as a cofactor, the cleavage of the Co-C bond constitutes the key catalytic step. The most intriguing features of this cleavage is …

Novel Bayesian Methodology For The Analysis Of Single-Cell Rna Sequencing Data., Michael Sekula

Electronic Theses and Dissertations

With single-cell RNA sequencing (scRNA-seq) technology, researchers are able to gain a better understanding of health and disease through the analysis of gene expression data at the cellular-level; however, scRNA-seq data tend to have high proportions of zero values, increased cell-to-cell variability, and overdispersion due to abnormally large expression counts, which create new statistical problems that need to be addressed. This dissertation includes three research projects that propose Bayesian methodology suitable for scRNA-seq analysis. In the first project, a hurdle model for identifying differentially expressed genes across cell types in scRNA-seq data is presented. This model incorporates a correlated random …

Preventable Hospitalization Among Type 2 Diabetes Patients In Kentucky Before And After Medicaid Expansion 2010-2017., Turky Jamil Arbaein

Electronic Theses and Dissertations

Objectives: 1) Analyze county level variation in T2DM-PH rates in Kentucky before ACA (2010-2013) and after the ACA (2014-2017). 2) Analyze the relationship between county level socioeconomic factors (income per capita, percentage of uninsured people, percent of urban population, primary care and general preventive offices, population aged 65 and above, median age, household income, percentage in poverty, and unemployment rate ) and county level T2DM-PH rates before (2010-2013) and after (2014-2017) ACA implementation in Kentucky. Method: This research was conducted in two phases: Phase one of this study estimated the county-level PH variation among T2DM patients across eight years (2010-2017), …

Analyzing The Effects Of Of Seasonal Land Cover And Precipitation On The Sediment Delivery Ratio Of An Agriculture Dominated Watershed., Jonah Liebman

Electronic Theses and Dissertations

Soil erosion is of escalating importance as increasing population and climate change have put increasing pressures on agricultural food production. Vegetation and precipitation are two factors that control the amount of soil erosion extant within a region. Sediment delivery ratios (SDRs) assess the ratio of soil eroded from a watershed system that is permanently removed from the system through stream sediment discharge. Using 1) river discharge and sediment concentration data and 2) the Revised Universal Soil Loss Equation (RUSLE), this thesis analyzes fluctuations in monthly SDRs for an average hydroclimatological crop-harvest season for the Senachwine Creek watershed, IL. Through calculating …

Electrochemical Reduction Of Carbon Dioxide And Carbon Monoxide For The Production Of Green Fuels And Chemicals., Jacob M. Strain

Electronic Theses and Dissertations

It has become apparent that closing the carbon cycle on this planet in order to mitigate disastrous consequences of runaway global warming has become one of the most pressing issues of our civilization. One of the ways we need to accomplish this goal is by finding news methods to generate fuels that will be carbon neutral. Renewable fuels and green chemicals will be a major component of closing the carbon cycle and restoring our planet’s ecosystem into a sense of balance. A method that can help achieve this goal is the reduction of CO₂. If CO₂ can …

Assessing Predictors Of Harmful Algal Blooms In The Western Lake Erie Basin., Katherine M. Weigle

Electronic Theses and Dissertations

Harmful algal blooms (HABs) have been a recurring issue in the Western Lake Erie Basin for more than a decade, despite policies implemented to improve the water quality of the Great Lakes. HABs describe algae that can be harmful to both ecosystem function and human health. The Western Lake Erie Basin, in particular, experiences Microcystis aeruginosa blooms in the summer months due to a multitude of factors leading to increased phosphorus and nitrogen loads in the contributing watersheds. In this study, lake water surface temperature, land cover, river discharge, and precipitation were examined as potential triggers for the occurrence and …

Using Active Learning Techniques To Improve Oral Health Literacy Among Children In Vulnerable Populations., Sarah Maria Downes

Electronic Theses and Dissertations

This thesis examines the effectiveness of an active learning approach in improving oral health literacy and attitude about science among at-risk students in the age range of third to sixth grades. This is done by designing and implementing a novel Oral Health Outreach module and video. Active learning components include engaging PowerPoint, oral hygiene discussion and demonstration, and finally a biofilm simulation, which utilizes glow-in-the-dark paint, dentoform models, and black lights that allow students to visualize the importance of plaque removal. Student comprehension of oral health concepts increased by an average of 52.4% on post-tests (n=61, p

The Effect Of Radium Partitioning On Hydrothermal Fluid Flow Determination Across Guaymas Basin, Charlotte R. Kollman

Electronic Theses and Dissertations

Analysis of the distribution of radium through sedimentary porewater profiles is a promising method for constraining diffuse discharge/recharge flow rates in hydrothermal settings; these parameters are critical for evaluating hydrothermal circulation and its chemical and biological implications. The short-lived 224Ra (T1/2 = 3.54 days) is an effective tracer due to its behavior in marine environments and changes in activity due to ingrowth/decay. However, the distribution of radium may be influenced by its varying solid:aqueous partitioning coefficients (Kd) and is critical to understand for any study utilizing radium as a tracer. This project explores Kd across the hydrothermally active Guaymas Basin …

Community Involvement In Flood Mitigation: A Survey-Based Approach In Marion County, Sc, Lillian C. Howie

Electronic Theses and Dissertations

Marion County is located in northern South Carolina between the Great Pee Dee and Little Pee Dee Rivers. Because Marion County was the location of severe flooding during hurricanes Matthew (2016) and Florence (2018), the South Carolina Floodwater Commission identified Marion County as a location for drainage system improvement by the Infrastructure and Shoreline Armoring Task Force. In order to align plans for drainage system improvement with the needs of the local communities, commission chairman Thomas Mullikin requested a community survey to gauge the residents' personal experiences and views on what changes should be made to address the problem. The …

Investigating Dorsal Fin Hydrodynamic Function Of The Lemon Shark, Negaprion Brevirostris, Vivian Lynn Turner

Electronic Theses and Dissertations

Many sharks are considered highly efficient swimmers. Their swimming efficiency is partially governed by their morphological features such as dorsal fins, which play a role in their hydrodynamics. Most sharks feature two dorsal fins that can vary in size and location over the body. The first dorsal fin has been shown to improve stabilization, maneuverability, and increase thrust production during swimming, whilst the hydrodynamic role of the second dorsal fin is largely unknown. An understanding of the hydrodynamic function of the dorsal fins can be utilized in engineering applications, e.g., to replicate fins on underwater autonomous vehicles to increase energy …

Temporal Transport Dynamics Of The Amazon River Plume Revealed Using Radium Isotope Analysis, Elana J. Ames

Electronic Theses and Dissertations

The Amazon River is the largest river by discharge in the world. It carries terrestrial nutrients into the Western Tropical North Atlantic via a buoyant freshwater plume, conveying water hundreds to thousands of kilometers away and driving critical biogeochemical cycles near the coast. Factors controlling the delivery of nutrients offshore by the plume are complicated and interconnected, yet these nutrients impact the foundation of phytoplankton community structures across the entire plume ecosystem. To better understand the temporal dynamics of this massive, highly influential region, we employ naturally occurring radium isotopes (223Ra, 224Ra, 226Ra, and 228Ra) to analyze mixing and transport …

A Qualitative Case Study To Investigate How Educators Promote Parent Collaboration And Involvement For African American Elementary Males With Learning Disabilities, Derek E. Pierce

Electronic Theses and Dissertations

The disproportionate identification and achievement gap of African American students and their peers are inexhaustible. African American students continue to lag tremendously behind their Asian and Caucasian peers. There is limited research of African American students with disabilities as it relates specifically to various components of special education. To understand the narrative surrounding African American males, their achievement, and the collaboration between their parents and educators, the researcher will evaluate various components in conjunction with educators’ composition and characteristics and various parent’s attributes and behaviors. The purpose of this qualitative case study is to investigate how educators promote parent collaboration …

Culturally Familiar Texts To Improve Reading And Writing Outcomes For High School English Language Learners: An Explanatory Sequential Mixed Methods Study, Taryn Courtney Robertson

Electronic Theses and Dissertations

Research suggests that culturally relevant pedagogical strategies are essential for improving culturally and linguistically diverse student achievement. However, there is little research about which specific strategies provide the largest impacts. And there is even less research on which strategies help LatinX English Language Learners achieve academic success in the areas of reading and writing. The purpose of this study was to determine the impact of utilizing culturally familiar text, as compared to culturally unfamiliar text, on reading comprehension and summary writing outcomes for secondary LatinX ELL students. This study sought to determine if there were statistically significant differences in reading …

Controlling Transition Metal-Catalyzed Alkyne Annulations Utilizing Polarized Ynol Ethers, Brandon L. Coles-Taylor

Electronic Theses and Dissertations

Transition metal-catalyzed alkyne annulations have developed into incredibly powerful synthetic tools over there the past quarter century. These reactions provide rapid access to important organic scaffolds such as indole, quinoline, isoquinoline, indene, and isocoumarin scaffolds. Transition metal mediated alkyne annulations have proven invaluable in synthetic fields, such as natural product total synthesis, by offering efficient pathways to otherwise synthetically difficult to access substrates.

Foundational works performed by chemist such as Larock, Ackermann, Satoh, and Miura have been established through relying upon the usage of symmetrical alkynes. When unsymmetrical alkynes are used in annulation processes mixtures of regioisomers are often isolated. …

Supporting The Affective Needs Of Gifted Readers: An Educational Criticism And Connoisseurship Study, Kimberly G. Freed

Electronic Theses and Dissertations

The purpose of this Educational Criticism and Connoisseurship Study was to analyze the perceptions of parents and teachers of reading supports and barriers on the affective development of gifted readers. The research questions that guided this study were: What do parents and teachers perceive as the most effective reading supports that promote the affective development of young gifted readers? What do parents and teachers perceive as the major barriers that hinder the affective development of elementary gifted readers? How can children’s literature be used to support or hinder the affective development of gifted readers? Participants of this study were parents …

Creating Life-Long Relationships With The Arts: A Caring Philosophical Approach, Jennifer Sparkman Bartee

Electronic Theses and Dissertations

It is important to understand how performing and visual arts teachers make their classes personally relevant for students. This study examines what the intentions of elementary performing and visual arts teachers who chose to teach with additional affective content. Four research questions were addressed in this study: 1) What are the instructional intentions of care-oriented elementary arts teachers? 2) What are the affective intentions of care-oriented elementary arts teachers? 3) How are these intentions actualized (or not actualized) within elementary arts classrooms? 4) What is the significance of these findings for elementary schools throughout the country?

To respond to these …

Navigating Uncharted Waters: An Exploration Of The Social And Emotional Competence Of First-Year Teachers, P. Daniel Riordan Jr.

Electronic Theses and Dissertations

This study is an exploration of the social and emotional competence of first-year teachers with particular attention directed to how these five core competencies influence the way new teachers navigate tension and exercise agency. Tension is embedded into the fabric of a teacher’s first year. New to the field and still learning the complex art of how to teach, novice teachers must navigate an unfamiliar context without a complete understanding of the potential threats to their well-being. The study explores tension experienced by first-year teachers and their social and emotional competence through classroom observations, interviews, and weekly voice memos with …

Examining Artifacts Of The Watershed Segmentation, Emily Jo Armitage

Electronic Theses and Dissertations

The watershed segmentation is an algorithm used to systematically track cell intercalary behaviors during germ band extension of the Drosophila embryo. Neighboring cells share a contracting vertical interface, called a T1, which continues contracting to a single point, a T2, and extending in the horizontal direction to create what is called a T3 interface (Fig. 1). Additionally, higher order vertices called rosettes occur when five or more cells meet at a common vertex. Simulated T2 events demonstrate that cell angle and not noise level in the image contributes to the incorrect detection of artifactual T1s in more acute angled cells …

Iron In Urban Air Pollution, Joseph R. Salazar

Electronic Theses and Dissertations

The processes by which iron is solubilized are largely unknown. There are three hypotheses on how iron is soluble: 1) iron is thought to undergo an acid based solubilization process, 2) iron undergoes an oxidation/reduction process and reduces iron(III) to the more soluble iron(II), and 3) iron is bound to organic molecules, which solubilizes it. The 3 hypotheses on iron water-solubilized are explored by presenting two individual studies.

The first study investigates iron directly from car exhaust. Exhaust particles were collected from 32 vehicles. Iron solubility in these samples ranged from 0%-82% with the average iron solubility being 30%. X-ray …

Building Community In The Classroom For Culturally Linguistically Economically Diverse Gifted Learners, Michelle Elaine Norberto-Whipple

Electronic Theses and Dissertations

The purpose of this study was to explore the perceptions of active community building in a classroom with culturally linguistically economically diverse (CLED) gifted learners in a semirural school district who underachieve. The central research question was, how does actively building classroom community interact with CLED gifted learners who underachieve? The research questions were the following: What are educators’ perceptions of CLED gifted learners who underachieve? How do educators implement community-building in the classroom? What are parents’ perceptions of actively building community in the classroom with CLED gifted learners who underachieve? The four primary categories that emerged were (a) meeting …

Quantification Of Mitochondrial Zinc Homeostasis And Analysis Of Zinc And Polyamine-Mediated Axonal Trafficking, Dylan Hunt Fudge

Electronic Theses and Dissertations

The zinc ion is an important emerging signaling molecule for biological processes. In this work we engineered improved zinc sensors based on our previously developed fluorescent sensor GZnP1 to provide sensors with a higher fluorescent readout, faster kinetics, and a superior sensitivity to zinc. We utilized these zinc sensors and further developed the sensors to answer questions pertaining to biological zinc. We showed the labile zinc concentration in the mitochondrial matrix was less than 1 pM while the labile zinc concentration in the cytosol and mitochondrial IMS were comparable (~100 pM) across four different cell lines. Using these zinc sensors …