Trained Immunity Enhances The Immune Response And Maintains Microbiome Diversity In Aging And Sepsis, P. Spencer Gill

Electronic Theses and Dissertations

The global population is rapidly aging. It is estimated that over the next thirty years, the number of individuals >60 years of age will increase by over a billion, and the number of individuals over age 80 may increase by 300 million. As humans age, our immune system becomes progressively weaker through a process called immune senescence. This age-related decrease in immune function increases susceptibility to infection and chronic diseases. Sepsis is a leading cause of death worldwide. Over the past two decades, there has been an increased incidence of sepsis which is due, in part, to our aging population …

Role Of Ataxia Telangiectasia Mutated Kinase In Western-Type Diet-Induced Cardiac Outcomes Under Basal And Ischemic Conditions, Mary Wingard

Electronic Theses and Dissertations

Ataxia-telangiectasia mutated kinase (ATM), a serine/threonine kinase, plays a role in DNA damage repair, redox sensing, and metabolism. In the heart, ATM contributes significantly in the myocardial infarction (MI)-induced cardiac remodeling with effects on fibrosis, hypertrophy, apoptosis and inflammation. This study investigates the role of ATM deficiency in 14 weeks Western-type diet (WD)-induced cardiac outcomes prior to and 1-day post-MI in a sex-specific manner using wild-type (WT) and ATM heterozygous knockout (hKO) mice. In male mice, ATM deficiency induced rapid body weight gain and preload-associated dysfunction, while WT mice displayed afterload-associated dysfunction 14 weeks post-WD. Myocyte apoptosis and hypertrophy were …

Genital Chlamydia Infection Is Influenced By The Female Sex Hormones Estrogen And Progesterone In Vivo, Amy Gail Gravitte

Electronic Theses and Dissertations

Chlamydia is the most common bacterial sexually transmitted infection in the United States and worldwide. It often goes unnoticed due to lack of symptoms and left untreated it can ascend the female genital tract to cause sequelae like pelvic inflammatory disease and irreversible tubal infertility. In reproductive-aged women, female sex hormones estrogen (E2) and progesterone (P4) concentrations fluctuate during the menstrual cycle and are influenced by hormonal contraceptives and hormone replacement therapy. E2 and P4 influence genital Chlamydia infection in women and mice, but these multifactorial interactions are not entirely mapped out. The complex interplay of E2 and P4 with …

A Phenomenological Study Examining How The Covid-19 Pandemic Changed The Way Teachers Use Technology To Deliver Instruction From March 2020 - May 2021, Dedra Lamb

Electronic Theses and Dissertations

The purpose of this qualitative study was to examine teacher perception of changes in the instructional delivery and learning opportunities via technology during and throughout the COVID-19 pandemic from March 2020 until May 2021. The COVID-19 pandemic has greatly affected educational organizations. School closures in March 2020 forced teachers to change their instructional delivery from an in-person platform to a virtual platform. This disruption to the delivery of instruction with the use of technology changed the way teachers plan for learning, delivery content, present learning activities, and assessment. The urgency required teachers to develop new strategies and experiment with adaptations …

Implementing Mindfulness Meditation To Reduce Nurse Burnout, Jannelle V. Sanchez

Electronic Theses and Dissertations

Abstract

There will be an estimated shortage of 100,000 nurses by the year 2022, with 500,000 seasoned nurses retiring. The nursing profession has recently experienced a devastating pandemic that left no time to combat the detrimental effects of burnout, specifically for intensive care nurses, who are the frontline workers of a physically and psychologically draining working environment conducive to burnout. Nurses experience burnout for a mixture of reasons, but mainly it is due to the lack of interventions to help them combat the disease. Mindfulness meditation represents an interventional tool that has been shown to reduce the negative stressors associated …

School Nursing Practice And Experiences During School Closures, Susie E. Birden Brown

Electronic Theses and Dissertations

This evidence-based quality improvement project was to evaluate public school nurses’ relationships and reaction to their new work environment during the closure of schools in Northern California resulting from the COVID-19 pandemic. This project addressed the change in school nursing, especially the nurses’ working environment. A stress management program was offered to the school nurses as an intervention to give them information on perceived stress. The stress management program included mindfulness and stress-relieving techniques over a 1-month period. The research methodology used in the project was a pre- and postintervention using the Perceived Stress Scale-10 (PSS) to measure if the …

Opioid Use Disorder: A Crisis Of Concern, Evangella Harville

Electronic Theses and Dissertations

According to a statement from the U.S. Centers for Disease Control and Prevention, between 2000 and 2017, approximately 700,000 people died from drug overdoses in the United States. Approximately 128 persons lose their life each day from an opioid overdose. The data for this study were collected by the vice president of operations at the study site from a preexisting database for 2019 and 2020. The participants were inpatient patients, 18 and older, both male and female, and were from all ethnicities. The study facility was located in Western New York and offered a 30-bed inpatient treatment center with 24-hour …

The Cultural Worldview Of Women Trafficked For Sex In The U.S: An Ethnonursing Study Exploring Health And Well-Being Beliefs, Values And Practices From An Emic Perspective, Christine Lepianka

Electronic Theses and Dissertations

Introduction: Women who have been trafficked for sex in the United State report that the health care they receive is not consistently helpful or tailored to their unique needs. The purpose and domain of inquiry (DOI) for this study was to discover, understand, and describe health and well-being beliefs, values, and practices of U.S. born women who have been trafficked for sex in the United States, in order to provide culturally congruent nursing care. Method: Leininger’s Culture Care Theory (CCT), ethnonursing research method (ERM), and enablers guided the researcher as she explored the DOI. Interviews were conducted with 11 key …

Multivariate Cluster Analysis Of The Teacher Stress Inventory (Tsi) Prior To And During Covid-19, Berenice Saez

Electronic Theses and Dissertations

The coronavirus disease 2019 (COVID-19) pandemic has caused a negative situation with no precedents in the education system of the United States (U.S.). To mitigate the spread of the virus, many school closures occurred nationwide, and schools transitioned from face-to-face instruction to a mixture of self-directed guide home education or online teaching. These drastic changes could be causing teachers, as many other professionals exposed to sudden adjustments (e.g., medical doctors/firefighters), to express high levels of stress, emotional burden, and anxiety. The purpose of this study is to compare the profiles of the teacher stress inventory (TSI) before and during COVID-19. …

Development And Validation Of Biosafety Climate Scale And Survey-Based Analyses Of Safety Perceptions In Biological Research And Teaching Laboratories At Public Universities In The Usa., Sivarchana Mareedu

Electronic Theses and Dissertations

Biosafety plays a key role in ensuring safety of researchers’ as well as the public from unintentional exposures to infectious agents. However, the occurrence of lab acquired infections, exposures, and safety lapses in biological laboratories underscores challenges in biosafety program management. The raise in emerging and reemerging infectious diseases and the ongoing COVID-19 pandemic reiterates the need for biosafety and research community to work together. Literature recommends the application of safety climate in measuring safety culture. The goal of this dissertation is to quantify biosafety climate and examine associated factors to understand the gaps between research and biosafety professionals in …

"When The Voices Of Children Are Heard": Evaluating The Biological Effects Of Socioeconomic Status On Children In Postmedieval London., Ashley R. Ezzo

Electronic Theses and Dissertations

As the most vulnerable members of society, children (and their treatment) may reveal important sociocultural and socioeconomic praxes throughout human history. During the Postmedieval period, children, especially low socioeconomic status (SES) individuals, participated in the workforce. By examining the demographic distributions and paleopathological profiles of juvenile skeletal remains from high SES (St. Bride’s Crypt and Chelsea Old Church) and low SES (St. Bride’s Lower and Crossbones) Postmedieval (1700-1850 CE) London cemeteries, we can better understand how SES impacted the biological health and mortality of children. While results indicated higher mortality risk associated with low SES circumstances, most differences in pathological …

Development Of 3d-Bioprinted Scaffolds For Oral Probiotic Application., Jhanvi Patel

Electronic Theses and Dissertations

Background: Porphyromonas gingivalis adheres to and invades gingival epithelial cells, resulting in decreased cell viability. Previous studies have indicated that probiotics are effective against dental pathogens; however, few approaches provide sustained-delivery of active agents in the oral cavity. Hypothesis: Probiotics will limit P. gingivalis effects on Telomerase Immortalized Gingival Keratinocyte (TIGK) cells and 3D-printed scaffolds will prolong probiotic release. Methods: Lactobacillus acidophilus, Lactobacillus reuteri, and Bifidobacterium bifidum were assessed with adhesion and antibiotic protection assays to limit P. gingivalis effects on TIGKs. Scaffolds were printed with a select probiotic and evaluated for release kinetics. Results: Free L.a., …

An Investigation Of The Impact Of Covid-19 Infection Control On Visual Art Installations In Hospitals With Pediatric Patients, Shannon Kimich

Electronic Theses and Dissertations

This study investigated the impact of COVID-19 infection control on visual artwork in pediatric hospitals and hospitals caring for pediatric patients. The study involved five hospitals in three southern America states and addressed visual artwork and art installations. The researcher administered an internet-based survey to participants in job functions related to hospital administration, environmental services, and project management across the facilities and received participation from 18 respondents. Additionally, telephone interviews were conducted with three participants with the job titles project manager/analyst, child-life specialist, and environmental services director. Survey questions were designed to examine visual art programs in selected children’s hospitals …

Predicting Covid-19 Behavior: Is It All Due To Political Orientation?, Caleb Potts

Electronic Theses and Dissertations

ABSTRACT

Adherence to COVID-19 preventing measures is becoming increasingly important as governments across the world realize COVID-19 is not an acute, but a chronic problem. There is, however, disagreement over to what extent COVID-19 is a problem. In the United States, the division appears to be primarily along party lines (Democrat and Republican[1]), though even within parties there is division. This division might be explained by differences in: the behavioral immune system and trust in government. Additional factors to examine include: personality, fear of COVID-19, and religious beliefs. The present study used previously validated self-report measures to assess …

Professional Ethics For College Health Services, Dessa Mrvos

Electronic Theses and Dissertations

College health services provide a crucial resource for campus communities in the United States, substantially affecting student health, safety, and wellbeing. The impact and quality of campus clinical services impact student life through varied approaches to the delivery of services, health innovations, and business models. The role that campus health services plays in dealing with risk mitigation is essential: well-established public health principles of disease prevention, health promotion, and health education are sustained and advanced by campus clinical health services. Despite the widely recognized importance of college health services, there is very little literature on the contribution of ethics related …

Sex-Based Differences In Microbiota Composition And Metabolomic Profiles Associated With Altered Macrophage Function And Protection From Lupus In Bwf1 Mice., James William Harder

Electronic Theses and Dissertations

Systemic lupus erythematosus (SLE) is a complex and an insidious disease that is still not completely understood, and has very few treatment options. Those that are available are ineffective and/or have serious side effects. Both genetic and environmental factors contribute to the susceptibility and resistance to SLE, and understanding the environmental factors underlying this disease could lead to more effective prevention and/or treatment strategies. Like most autoimmune diseases, SLE is much more prevalent in females than males. While there are numerous factors that contribute to this lupus susceptibility, there is increasing evidence that the microbiota can strongly influence lupus progression, …

Particulate Matter Exposure And Community-Acquired Pneumonia Mortality Among Hospitalized Adults With Pneumococcal Pneumonia: Incidence Study (Happi) Participants., Jack Anthony Pfeiffer

Electronic Theses and Dissertations

The role of air pollution as an agent of elevated morbidity and mortality has come under increasingly intense scrutiny in recent years. As the body of literature grows, so too does our understanding of how extensive the harm of such pollutants can be. Particulate matter (PM) particularly harmful due to its small size, ability to travel great distance while airborne, and capacity to infiltrate numerous bodily systems, often to the detriment of those exposed. Besides being associated with increased mortality risk in more heavily exposed populations, PM exposure has also been associated with complications in the respiratory, cardiovascular, and nervous …

Harnessing The Power Of Trained Immunity In The Setting Of Pancreatic Cancer: A Novel Mechanism Of Immune Trafficking And Tumor Control., Anne Elena Geller

Electronic Theses and Dissertations

Despite the success of immunotherapy in many types of cancer, pancreatic adenocarcinoma (PDAC) has yet to benefit. Innate immune cells are critical to antitumor immunosurveillance and recent studies have revealed that these populations possess a form of memory, termed trained innate immunity, which occurs through transcriptomic, epigenetic, and metabolic reprograming. Though trained innate immunity has mostly been investigated in the context of infection, the induction of trained innate immunity could also protect against tumors, and specifically pancreatic tumors. Here, we demonstrate that yeast-derived particulate β-glucan, a known inducer of trained immunity, traffics to the pancreas following IP administration. This causes …

Std Prevention: An Educational Intervention For Exotic Dancers, Maria Delurdes Dehoyos

Electronic Theses and Dissertations

The Centers for Disease Control and prevention reported nearly 2.3 million STD cases diagnosed in 2017, and the number is continuing to rise. San Antonio, Texas, is ranked third in the state of Texas with the highest STD rates and leading in the state with congenital syphilis. There is an increased risk for contracting STDs working in the sex industry. There are little to no studies pertaining to any educational interventions offered to exotic dancers to increase personal knowledge on STDs. This research has focused on an educational intervention offered by a nurse practitioner to exotic dancers in two adults-only …

Anger Bias In The Evaluation Of Crowds, Diana Mihalache

Electronic Theses and Dissertations

Although people are good at classifying emotions, they also make mistakes. These errors tend to be negatively biased and potentially serve a protective function. Research on biases in emotion perception has largely focused on perception of individual faces and little is known about biases in evaluations of crowds. In the first investigation, I conducted six experiments, evaluating anger bias—a tendency to judge facial expressions as angry—in the context of single faces and emotionally homogenous crowds. I found that observers were biased to classify faces as angry, especially when evaluating crowds. This amplified bias emerged in the context of perceptual uncertainty …

A Biophysical Investigation Of Hiv-1 Envelope Cytoplasmic-Tail Mediated Retention In The Gag Lattice, Nicholas Scott Groves

Electronic Theses and Dissertations

The infectivity of Human Immunodeficiency Virus is critically dependent on successful incorporation of the trimeric viral envelope glycoprotein, Envelope (Env), into Gag lattice assembly sites on the host-cell plasma membrane during virus biogenesis. The mechanistic nature of interaction between Gag, the structural protein, and Env, the viral spike, are dependent on the Gag matrix (MA) and Env cytoplasmic tail (CT) domains. Env laterally diffuses on the host cell plasma membrane until encountering a budding Gag lattice, where it is retained until particle scission and release. The mechanism of Env entry and retention in the lattice remains elusive and enigmatic. Here …

Optimized Microbial Recombinant Production Of Hiv-1 Anti-Envelope Antibody Fragments With Applications To Single Particle Tracking Of Virus Assembly, Merissa Michelle Bruns

Electronic Theses and Dissertations

In my findings, I have established a set series of protocols to recombinantly produce, purify and apply various fluorescent probes in vitro for the fluorescent labeling and study of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains insufficient knowledge about the molecular dynamics and interactions of HIV-1 Env protein with its counterpart, Gag, on the inner host cell surface during assembly of a mature virus particle. There also remains an insufficient amount of data for the understanding and clarification of the mechanism of action of a known host cell HIV-1 restriction factor, …

Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez

Electronic Theses and Dissertations

This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …

Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan

Electronic Theses and Dissertations

This study aimed to evaluate the transcriptomic profile of both corium tissue from lactating dairy cows experiencing sole ulcers and skin lesions from lactating dairy cows diagnosed with digital dermatitis (DD). Hoof biopsies were performed in multiparous Holstein dairy cows selected based on their condition as clinically healthy or diagnosed with sole ulcers (n=7/group). Similarly, skin biopsies were taken from the center of active (M2/M4.1) DD lesions or non-active (M0/M4/M1) from multiparous Holstein dairy cows (n = 7/group) to assess the impact of DD on the skin transcriptome via RNA-seq analysis. All RNA samples were sequenced using Illumina, NovaSeq S4 …

An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler

Electronic Theses and Dissertations

Within the last century, bighorn sheep (Ovis canadensis) in the badlands ecosystem of western South Dakota have been subjected to complete extirpation, reintroduction, disease die-offs, genetic bottlenecking, and population augmentation. Subsequently, the population in Badlands National Park (BNP) appears to have recovered, but it was unknown to what degree past events had influenced the population. From 2017-2019, we conducted research on 5 subherds within 2 management units in BNP to 1) survey for the presence of respiratory pathogens and estimate the prevalence of other potentially infectious diseases; 2) assess adult and lamb survival and cause-specific mortality; 3) estimate population size …

Isolation Of Marker Compounds From Copaiba Essential Oil And Mimosa Pigra For Quality Evaluation Of Dietary Supplements, Mohammed Hawwal

Electronic Theses and Dissertations

Natural products have been used as a primary means of treating illness since ancient times, and they remain an important pool of U.S. FDA approved drugs. Approximately 33% of all current pharmaceutical medications discovered between 1981 and 2014 are either natural products or derived from natural products (Newman and Cragg 2016). The process of isolating specialized metabolites from medicinal plants has led to the development of particularly important medicines worldwide (Rakotomalala, Agard et al. 2013) such as Taxol® from Taxus brevifolia Nutt. (Taxaceae) and salicylic acid from the bark of Salix spp. This research is composed of five chapters; the …

Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam

Electronic Theses and Dissertations

BVDV is a major pathogen of ruminants that causes immune dysfunction and lead to secondary bacterial infection. The underlying mechanism of this dysfunction is not well understood. Based on previous results, we believe that macrophages play a central role in BVDV-associated immune dysfunction. Previously, we have shown that the infected monocyte-derived macrophage (MDM) supernatant of the high virulent strains of BVDV significantly induced lymphocyte apoptosis. In the current study, we performed experiments to identify the apoptotic factors in the infected MDM supernatants in vitro. Also, we have extended our investigation to include testing the impact of infected macrophages on neutrophil …

Responses Of Myxobacteria To Plant-Related Chemosignals Suggests Their Roles In The Rhizobiome, Barbara Iyare Adaikpoh

Electronic Theses and Dissertations

In the rhizosphere, plants curate and maintain distinct rhizobiome, some of which serve as secondary defense mechanisms against pathogenic microorganisms. Additionally, in response to biotic stress, plants generate phytohormones which regulate signaling pathways to activate systemic resistance. These phytohormones contribute to the chemical space within the rhizosphere in addition to other low-molecular-weight metabolites used for cell-to-cell communication within species and across kingdoms. However, little is known about the influences of stress-related phytohormones on beneficial bacteria even though biocontrol strategies abundantly explore plant-beneficial microorganisms to manage pathogens in agricultural systems. Myxobacteria are competent predators of plant pathogens and have demonstrated responses …

Chronology Of The Fatherland Site, Daniel Shawl

Electronic Theses and Dissertations

A recently discovered mound (Mound E) at the Grand Village of the Natchez Indians (22AD501, the Fatherland Site) dates to the Foster phase of the Mississippian period (1300-1500 CE). This new chronology is stems from analysis conducted on ceramic assemblages from Robert S. Neitzel’s (1965) excavations and ceramic assemblages from Tony Boudreaux’s (2019) excavations at the site. Frequency seriations, non-metric multidimensional scaling, and radiocarbon dates suggest that Mound E was constructed and in use several hundred years prior to European contact at the site. The results of this body of research brings new insight into a portion of history of …

Effectiveness Of An Educational Intervention To Reduce Ageism In Undergraduate Students In An Entry-Level Nutrition Class, Ann Irvin Armstrong

Electronic Theses and Dissertations

Although the older adult population continues to increase, ageism remains an issue in the healthcare system and is prevalent among nutrition-related professionals. The aim of our study is to is determine the effectiveness of an educational intervention to reduce ageism among pre-healthcare professionals including Nutrition major students. Undergraduate students enrolled in an entry-level nutrition class were recruited and randomized into an intervention (INT) group (n=30) and a control (CON) group (n=29). The online educational intervention consisted of pre-recorded lectures and videos regarding ageism and myths regarding aging for the INT group, and on cultural competency and biases (other than ageism) …