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Characterization Of Fundamental Parameters That Modulate Acoustofluidic-Mediated Molecular Delivery To Cells., Connor Sterling Centner

Electronic Theses and Dissertations

Cell-based therapies represent the latest biotechnological revolution in medicine, but significant limitations exist in non-viral intracellular delivery techniques during manufacturing of cell therapies. To address these limitations, a novel acoustofluidic device was developed to deliver ultrasound waves into a flow chamber for rapid molecular delivery to human cells. Our acoustofluidic device delivers biomolecules by inducing expansion and collapse of exogenous gas-filled microbubbles (“cavitation”), which enhances molecular delivery to nearby cells. Experimental studies were conducted to characterize key parameters that influence acoustofluidic-mediated molecular delivery to human cells. A clinical ultrasound transducer was generally utilized with varying cationic microbubble concentrations. Intracellular delivery …

"Death Can't Touch Them Now": Aids Response And Memorialization In Louisville, Kentucky, 1982-1992., Olivia A. Beutel

Electronic Theses and Dissertations

This thesis aims to address the role of the queer community in Louisville, Kentucky during the AIDS epidemic. Beginning with the first reported AIDS death in the city in 1983 throughout the 1980s, dialogue focused on those living with AIDS, specifically on education for prevention and aid to those afflicted by the disease. Individuals in the queer community—gay men, lesbians, bisexual men and women, transgender men and women, and others—created resources that were not being provided by the larger city government. Then, in the 1990s, national attention to the AIDS Memorial Quilt encouraged people to participate in rituals of commemoration, …

Meat The Future., Xin Chen

Electronic Theses and Dissertations

This research focuses on the subtle relationship between human life, the environment, and other animals that we live among every day. Under the mainstream social background of capitalism and a commodity economy, animals are the victims of constant consumption and exploitation. However, meat is a basic substance for human nourishment, especially in certain cultures. As a result, meat becomes an important part of the commodity economy in capitalist societies, wherein the supply must be filled to accommodate society’s demand. This research formed an exhibition using glass and ceramic artwork using a playfully satirical tone to offer a crucial alternative: how …

Examining The State Of Substance Use Treatment Among Youth And Adults In The United States, Esther Adejoke Adeniran

Electronic Theses and Dissertations

In the United States (US), substance use, misuse, and substance use disorders are significant public health problems. Additionally, the difference between needing substance use treatment (SUT) and receiving treatment is becoming more pronounced. Therefore, the aims of this dissertation include, 1) to synthesize evidence of barriers and facilitators to integrating SUT into mainstream health care (MHC) after the Affordable Care Act was nationally implemented in 2014, 2) to examine the rate of treatment completion and dropout, along with its associated factors, among youth who received Medication-Assisted Opioid Therapy (MAT), 3) to determine the relationship between prior treatment episodes and length …

Genomic Tools And Models For Investigating The Role Of Germline Diversity In Mouse Antibody Repertoire Development., Justin T. Kos

Electronic Theses and Dissertations

Given the diversity and complexity within immunoglobulin (IG) loci, effective mouse models first require characterization of intra-strain differences and construction of high-quality reference assemblies for IG loci in several representative strains. To understand light chain germline diversity across biomedically significant mouse strains, we profiled the expressed IGK and IGL repertoires of 18 commonly used laboratory mouse strains using AIRR-seq. Across strains, we observed germline IGKV sequences shared by three different IGK haplotypes and a more conserved IGLV germline repertoire among common laboratory strains. Pacific Biosciences (PacBio) Single-Molecule Real-Time (SMRT) sequencing was used to sequence and assemble bacterial artificial chromosomes (BAC) …

Fabrication And Characterization Of Lactobacillus Crispatus Containing Bioprints For Bacterial Vaginosis Application., Anthony J. Kyser

Electronic Theses and Dissertations

Bacterial vaginosis (BV) is a condition in which healthy lactobacilli are replaced by an overabundance of pathogenic bacteria in the female reproductive tract. Current antibiotic treatments often fail to “cure” infection, resulting in recurrence in more than 50% of women, 6 months post-treatment. Recently, probiotics have demonstrated promise to restore vaginal health; however, as with other active agents, delivery requires once-to-twice daily administration. Recently, three-dimensional (3D)-bioprinting has enabled the fabrication of well-defined cell-laden architectures with tunable agent release, thereby presenting a novel approach with which to deliver probiotics. One promising bioink, gelatin alginate, was selected for study, due to its …

Facilitators To Becoming And Remaining Open Defecation Free In Uganda: Implications For Community-Led Total Sanitation Programming, Danette Lee Cagnet

Electronic Theses and Dissertations

Poor sanitation behaviors, including open defecation, present risks for diarrhea, which is credited with 600,000 deaths a year in children under age five. Although CLTS is a solution to ending open defecation, more than half of the investment in producing open defecation free (ODF) communities is lost during the maintenance phase. The purpose of this qualitative, case study was to understand stakeholder perceptions of the facilitators and hindrances to becoming ODF certified and maintaining ODF status and sanitary behaviors in a small village community located in Uganda, East Africa. The researcher held individual interviews and in-person focus group discussions to …

The Intersection Of Traumatic Brain Injury And Homelessness, Stephanie A. Chassman

Electronic Theses and Dissertations

The rates of TBI are significantly higher among individuals experiencing homelessness compared to the general population. Up to half of individuals experiencing homelessness may have a TBI. Accurate prevalence rates of TBI among individuals experiencing homelessness are difficult to obtain due to different methods of sampling participants and differing definitions of TBI; therefore, estimates may be underrepresented. Despite past research that has examined the relationship between TBI and homelessness, there are specific gaps in knowledge such as correlates and risk factors of TBI among individuals experiencing homelessness. This three-manuscript dissertation attempts to address these gaps in knowledge.

The first manuscript …

Developing, Refining, And Testing The Intersectional Model Of Service Use: A Transgender And Nonbinary-Specific Model Of Healthcare Access, Jarrod Call

Electronic Theses and Dissertations

Transgender and nonbinary (TNB) people are more likely to experience negative health outcomes than cisgender (i.e., not TNB) people, but less likely to access healthcare services due to the systemic forces of transphobia and cisgenderism. Despite this, there are few theoretical models of healthcare access specifically designed for TNB people. This three-paper dissertation addresses this gap by developing, refining, and testing the Intersectional Model of Service Use (IMSU), a population-specific framework for researching TNB healthcare access. Manuscript one describes how the initial IMSU was developed from an integrative review of currently available TNB healthcare access literature. This initial IMSU was …

Framing, Advice-Seeking, And Medical Trust In The R/Keto Community: An Analysis Of The Medical Flair On R/Keto, Sabrina Finke

Electronic Theses and Dissertations

First created in 1921, the ketogenic diet was used as a medical intervention for children suffering from severe epilepsy. However, in recent years, this highly restrictive diet has gained mainstream popularity for a number of illness management, wellness, and weight loss uses. Despite its rise in use and popularity, mainstream medical opinion still views the ketogenic diet as a last resort for severe conditions. Using a content analysis of posts tagged with the “medical” flair on the subreddit “r/Keto,” this thesis found commonalities among posts which praised the ketogenic diet and shared positive personal anecdotes were the most likely to …

Understanding The Impact Of Human Germline Single-Nucleotide Variants, Samuel Adjei

Electronic Theses and Dissertations

All genetic variations in the human genome are due to mutations, and therefore determining the impact of the different categories of mutations, particularly nonsynonymous single nucleotide variants (SNVs) which are the most abundant in the population is essential for understanding the genetics of human diseases. Generations of mutation studies have focused on a relatively small number, partly due to technological limitations. However, advances in next-generation sequencing have allowed for empirical assessments of genome-wide mutations. genome sequence of any individual contains extensive protein-altering genetic variation (missense mutations) and of which only a few are unambiguously deleterious. Characterizing the phenotypes of these …

Emerging Respiratory Viral Pathogens, April Nelsen

Electronic Theses and Dissertations

Respiratory disease is arguably the most important health concern for the production animal industry [1-3]. Respiratory problems accounted for the highest mortality both in the swine and beef industries [1, 4]. Respiratory pathogens remain the most vital for swine and bovine research and disease monitoring [2, 5-7]. As pathogens, in particular viral pathogens, mutate, novel disease-causing viruses emerge, and there becomes an increasing concern and need for identification with control perimeters. One frequently identified disease syndrome is Porcine Respiratory Disease Complex (PRDC). PRDC is characterized by pneumonia of mixed respiratory infections with contributions from the environment and management practices. The …

Inevitable Mortality And The Incessant Momentum Of Medicalized Death, Clifford Mckay

Electronic Theses and Dissertations

Atul Gawande, MD, affirms the inevitability of human mortality and the end of life in natural death. He decries current medical delivery’s incessant momentum toward medicalized death and recommends a meaningful conversation between doctor and patient to chart a course of care that supports what is important to the patient. But the tradition of a doctor’s dominance and a patient’s submissiveness disrupts the conversation. New definitions of both doctor and patient, and the intent to respect a patient’s autonomy, will facilitate decision-making and support patients’ values at the end of life, diminishing the momentum of medicalized death.

Acceptability And Feasibility Of Healthcare Communication Considerations For Altering Perceptions Towards Influenza Vaccine: A Qualitative Study, Saumil Jadhav

Electronic Theses and Dissertations

Background

Influenza (influenza) is a contagious disease, causing thousands of deaths every year. The Advisory Committee on Immunization Practices (ACIP) recommends routine annual influenza vaccination for all people over 6 months of age who do not have contraindications, yet the vaccination coverage for 2018-19 season was 49.2%, around 20% less than the goal set by Healthy People 2030 of 70% coverage. The national foundation of infectious diseases survey showed that 51% of respondents do not think the flu vaccine works, 34% are concerned with the side effects from the vaccine, and 22% are concerned about getting flu from the vaccine. …

Willingness To Use Hiv Pre-Exposure Prophylaxis (Prep) And Preferences For A Pharmacy-Based Prep Program Among Men Who Have Sex With Men, Swarnali Goswami

Electronic Theses and Dissertations

The purpose of this dissertation was to: 1) estimate willingness to use PrEP (WTUP) among men who have sex with men (MSM) using meta-analysis; 2) to assess patterns of minority stressors, HIV/AIDS acquisition stress and PrEP stigma among MSM and evaluate the association of such patterns with WTUP, using latent profile analysis; and 3) to elicit MSM’s preferences for a community pharmacy-based PrEP delivery program, using a discrete choice experiment.

First, a meta-analysis using a random-effects model revealed a moderate pooled prevalence proportion for WTUP of 0.58 (95% CI: 0.54-0.61) (or 58 out of 100) among PrEP naïve MSM estimated …

Progress In Natural Product-Inspired Drug Discovery, Md Imdadul Huque Khan

Electronic Theses and Dissertations

Natural products and product-derivatized molecules are significant sources for drug discovery and development, and many have been discovered and developed to treat various diseases, including cancer, infectious diseases, neurological disorders, etc. This dissertation reports on the progress of our natural product-inspired research in the discovery of new compounds to treat cancer, infectious diseases, and neuroHIV.

We discovered many glutamine-based compounds and prodrugs that are effective in suppressing the growth of several breast cancer cell lines without introducing cytotoxicity in a non-cancerous cell line. Our lead compound has shown good exposure to the brain, and several compounds were also found to …

A Novel Crispr/Cas Gene Editing System For Plants Which Bypasses Genetic Transformation And Tissue Culture, Aastha Acharya

Electronic Theses and Dissertations

CRISPR/Cas system is a powerful gene-editing tool that has revolutionized gene editing in many ways. However, the current delivery methods for using CRISPR/Cas system inn plants have some drawbacks that have greatly limited their routine application in plant research and improvement. Specifically,, the methods heavily rely on either genetic transformation for delivering Cas and/or its gRNA coding sequences into the targeted cells or tissue culture, or both for recovering the whole edited plants. To overcome these drawbacks, we have developed a simple, easy to use yet effective system for CRISPR/Cas gene editing in plants that can bypass genetic transformation and …

Identification And Characterization Of Candidate Rumen Bacterial Species From Beef Cattle With The Potential For Metabolizing Lipids, Cheyenne E. Hron

Electronic Theses and Dissertations

Ruminants and the microorganisms within their rumen have an intricate symbiotic relationship. Ruminants are able to convert plant materials that are indigestible to humans into meat, milk, and fiber for human consumption and use. Supplementing lipids in diets fed to beef cattle could be beneficial for the host animal as lipids are a higher density energy source. However, they can be detrimental to rumen microorganisms. While much research has been involved in elucidating the composition of the ruminal microbiome, knowledge of the effects of lipids on the ruminal microbiome has been quite limited. In this context, we took advantage of …

Polymeric Nanovaccine Delivery System For Influenza Vaccine, Chaitanya K. Valiveti

Electronic Theses and Dissertations

Vaccines are the most efficient and cost-effective method for preventing illnesses caused by infectious pathogens. Even though the great success of vaccines over decades, the development of safe and robust vaccines is still essential for emerging new pathogens, re-evolving old pathogens, and improving the insufficient protection given by existing vaccines. One of the most critical strategies for developing effective new vaccines is selecting and using a suitable adjuvant or immune stimulant. Immunologic adjuvants are essential for improving vaccine potency by enhancing the immune response of vaccine antigens. The amount of antigen could be spared with improved potency, especially during mass …

Perceptions Of Black First-Generation College Students Regarding The Impact Of Recruitment Practices At A Predominantly White Institution During The Covid-19 Pandemic, Deann Handy Walter

Electronic Theses and Dissertations

In 2020, the coronavirus disease (COVID-19) plagued the United States, becoming one of the greatest disruptions to the U.S. higher education system in history. Affecting every aspect of institution’s operations, prospective fall 2020 and beyond students were impacted greatly by the interruption of traditional college recruitment practices which would have occurred as they made their enrollment decisions. Most affected by this interruption were students of underrepresented groups. This dissertation focuses particularly on Black first-generation students and their perceptions regarding the impact of recruitment practices at a Predominantly White Institution during the COVID-19 pandemic. A qualitative study is proposed as the …

The Shades Of Immigration: How Skin Tone Shapes Attitudes Towards Venezuelan Immigrants In Latin America, Madison Hamilton

Electronic Theses and Dissertations

Venezuela is currently home to one of the most profound economic crises in modern-day Latin America and the most severe in any non-war-ridden country in recent years. Due to this, mass amounts of Venezuelan immigrants have left to countries such as Colombia, Chile, Ecuador, and Peru. This immigration within Latin America, with similar culture and language, leads to the question of if and how does skin tone predict attitudes towards Venezuelans in several Latin American countries? This study uses data from the 2018/19 LAPOP data survey to assess the link between skin tone and support for Venezuelan immigrants. Results of …

Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain

Electronic Theses and Dissertations

This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …

A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud

Electronic Theses and Dissertations

Pneumonia is a major factor affecting populations of free-ranging bighorn sheep (Ovis canadensis) across western North America. Pneumonia can occur in large-scale epizootics, during which greater than half of the population typically dies. After these epizootics, surviving ewes continue to conceive and bear lambs. However, lamb recruitment may remain low due to periodic or annual pneumonia outbreaks causing high lamb mortality rates, sometimes greater than 90%. Our study focused on the Rapid City, South Dakota bighorn sheep (BHS) herd that has recorded pneumonia-induced population decline since 2009. The first objective was to improve lamb health and survival by identifying and …

Connecting Human Dignity, Non-Violence, And Environment For Healthcare Ethics In Nigeria, John Mark Ogu

Electronic Theses and Dissertations

Abstract

Connecting Human Dignity, Non-violence, and Environment in Health Care for Nigeria

By

John Mark Chinaemerem Ogu

December 2021

Dissertation supervised by Peter Ikechukwu Osuji, Ph.D.

This dissertation presents a global public health ethical approach based on social justice that connects human dignity, non-violence, and the environment in Nigeria. Recently, different places globally are witnessing uprisings and violence; some of these are linked to environmental scarcity. These are threats to global health, peace, development, and security because violence of any type or form is a global public health and security menace and an affront to human dignity. Environmental scarcity, especially …

Q-Griffithsin Interactions And Utility For The Prevention And Treatment Of Mucosal Infections., Henry Nabeta

Electronic Theses and Dissertations

Griffithsin (GRFT) is a carbohydrate binding agent (lectin) that was originally identified in the red alga Griffithsia sp. Q-Griffithsin (Q-GRFT) is an oxidation stable analog of GRFT. GRFT has demonstrated inhibitory activity against HIV-1, Coronaviruses, Hepatitis C, influenza and Ebola viruses. The broad-spectrum activity suggests the potential utility of this lectin in a wide range of viral infections. However, the lectin’s activity in mucosal infections has not been extensively studied. Using in vitro, ex vivo and in vivo assays, we have demonstrated that Q-GRFT maintains the ability to bind glycosylated ligands following incubation in murine, macaque and human rectal …

Black Codes Re-Envisioned: The Dred Scott Majority Opinion As An Antiblack Performative Speech Act., Tiffany Dillard-Knox

Electronic Theses and Dissertations

This dissertation is a discursive analysis of the decision in the Dred Scott v Sandford, 1857 case written by Chief Justice Roger Taney. It begins with an overview of the literature on performative speech acts, focusing on the aspects of performatives that relate to Louis Miron and Jonathan Xavier Inda’s thesis that race is a performative speech act. Breaking from their use of race as the analytic, this analysis is situated within a black/nonblack paradigm. This provides a framework that focuses on the unique ways in which the discourse of the text enacts, accumulates and renders blackness fungible. The …

Exploring Service Provider Perspectives On Facilitators And Barriers To Needle Exchange Program Participation By Females Who Inject Drugs., Tammi Alvey Thomas

Electronic Theses and Dissertations

As the opioid epidemic lingers on across the country, many areas have set up harm reduction strategies such as needle exchange programs (NEPs) to combat the long-term consequences of injection drug use (IDU). Males and females face a plethora of health issues associated with injection drug use such as human immunodeficiency virus (HIV) and hepatitis C. While males comprise the largest portion of the injection drug use population, most research is gender neutral, which makes it difficult to discern issues specifically related to females inhibiting our ability to design interventions and procedures targeted to address their needs. Females require varying …

Understanding Wellbeing In Refugee Youth From The African Great Lakes Region., Victory Osezua

Electronic Theses and Dissertations

Young refugees are a vital and growing population in the United States that experience health inequities that make them vulnerable to adverse health outcomes. The health inequities that are evident between refugees and the general population elucidate the disparate power relations between the outcomes of the colonial past and postcolonial present. Although research shows that colonialism and their more recent experience of racism may have adverse long-term impacts on young people's health, it is essential to understand the contexts that shape their wellbeing. While the westernized model of wellbeing focuses on the individual, this study prioritizes the prevention of illness …

The Association Between Depression And Anxiety With Covid-19 Outcomes., Erica Miller

Electronic Theses and Dissertations

Previous studies have shown that mental disorders affect COVID-19 mortality. This study investigated the effect of depression and/or anxiety on COVID-19 outcomes. Depression/anxiety was defined by actively taking medication and/or diagnosis. The outcomes were ICU admission; ventilation; mortality; and time to mortality. Of 698 hospitalized patients, there were 204 (29%) defined to have either depression or anxiety. There were 109 deaths, and of those, 52 (48%) were diagnosed with depression/anxiety. Multivariable logistic regression and Cox proportional hazards models were used to examine associations. ICU admission and ventilation were not significantly associated with depression/anxiety. Depression/anxiety was associated with mortality (OR: 1.84, …

The Impact Of Statin Use On Outcomes Of Diabetic Adult Patients Hospitalized For Community Acquired Pneumonia., Joel Lanceta

Electronic Theses and Dissertations

BACKGROUND: Statins, a class of drugs that treat hyperlipidemia, may have an immunosuppressive effect for patients with community acquired pneumonia (CAP). Retrospective and in vitro studies have suggested an immunomodulatory, antioxidative and anticoagulant effects from statin use in patients with Type 2 Diabetes Mellitus (T2DM) hospitalized for CAP. Prospective studies that have tested any effect of statin therapy on patients with T2DM and CAP have not been found literature. To date, prospective studies showing of the effects statin therapy may have on T2DM patients hospitalized for CAP are not available. METHODS: This dissertation is a secondary analysis using deidentified data …