Digital Commons Network^™

Innate Immunity, The Hepatic Extracellular Matrix, And Liver Injury: Mathematical Modeling Of Metastatic Potential And Tumor Development In Alcoholic Liver Disease., Shanice V. Hudson

Electronic Theses and Dissertations

The overarching goals of the current work are to fill key gaps in the current understanding of alcohol consumption and the risk of metastasis to the liver. Considering the evidence this research group has compiled confirming that the hepatic matrisome responds dynamically to injury, an altered extracellular matrix (ECM) profile appears to be a key feature of pre-fibrotic inflammatory injury in the liver. This group has demonstrated that the hepatic ECM responds dynamically to alcohol exposure, in particular, sensitizing the liver to LPS-induced inflammatory damage. Although the study of alcohol in its role as a contributing factor to oncogenesis and …

Cd11b Regulation Of B Cell Receptor Signaling In Health And Systemic Lupus Erythematosus., Paul Lorenzo Dascani

Electronic Theses and Dissertations

Loss of function mutation in CD11b has been associated with incidence of systemic lupus erythematosus (SLE), a disease driven by B cell production of pathogenic autoimmune antibody. Our previous work has revealed the ability of CD11b to regulate B cell receptor (BCR) signaling and control autoimmune disease in mice. However, how CD11b regulates the immune response under normal conditions remains unknown. Through the use of a CD11b knockout model, we demonstrated that CD11b-deficient mice have an elevated antigen-specific humoral response in a Th2 type immunization. Deletion of CD11b resulted in elevated serum IgM and IgG antibody, increases in antigen-specific germinal …

Stalkers : What Are They Thinking?, Bethany Lynne Keller

Electronic Theses and Dissertations

Stalking became a prominent term in U.S culture in 1990 after a celebrity was murdered by a stalker in 1989. While media portrays stalking as a violent crime, often directed towards celebrities and prominent political figures, this is a much more common occurrence than people are aware, most often directed at women and perpetrated by men. Stalking often consists of the milder end of the continuum of behaviors, such as spying and leaving gifts for the target, and is often perpetrated by an individual the target knows, such as an acquaintance of an ex-intimate. Forty-eight percent of stalkers fall in …

Frederic Lindley Morgan : Gentleman Architect And Reluctant Modernist., Lisa Grace Carpenter

Electronic Theses and Dissertations

Frederic Lindley Morgan, a major architect in Louisville,Kentucky, was born in Loda,Illinois in 1889. Morgan, in partnerships with other architects throughout his career, designed a variety of projects including homes, schools, churches, commercial buildings and even an airport. Morgan worked up until he died, in 1970, in Louisville. What makes Mr.Morgan's work distinctive is not the timelessness of his designs, nor the fact he was dubbed "Louisville's society architect," nor his lengthy 50 plus year career. Morgan had the ability to take his training, in combination with his talent and savvy business sense, and work successfully in a variety of …

Fostering Compassionate Care For Persons With Alzheimer's Disease In Nursing Facilities., Sam Gilbert Cotton

Electronic Theses and Dissertations

The aim of this dissertation study was to examine the impact of the implementation of a new Compassionate Care (CC) curriculum on the quality of care provided by Certified Nursing Assistants (CNAs) to residents with Alzheimer’s disease (AD). More specifically, this dissertation used Kirkpatrick’s model of evaluation to assess the reactions, learning, and behavior change of the CNAs exposed to the curriculum, and ultimately the impact of the curriculum on the stress levels of residents with AD. To accomplish this, we had two studies that aligned with the Kirkpatrick model of evaluation. For Study #1, the evaluation of the compassionate …

Cholesterol Metabolism And Statin Effects On An Fh Class Ii Ldl-Receptor Mutation., Linda Omer

Electronic Theses and Dissertations

Familial hypercholesterolemia (FH) is a common genetic disease and has been studied with the aim of finding a curative measure for decades. FH is caused by mutations in the low-density lipoprotein receptor (LDLR) resulting in defects in LDL-cholesterol (LDL-C)-receptor mediated endocytosis and development of premature cardiovascular disease (CVD). Here I describe the use of a corrected and non-corrected LDLR FH cell model to investigate receptor-mediated endocytosis and statin effects. For these studies, we reprogrammed FH fibroblast cells to induced pluripotent stem cells (iPSC) and confirmed their pluripotency and ability to differentiate to hepatocyte-like cells (HLC). A clustered regularly interspaced short …

Microaggressions In Lgb Individuals: The Protective Role Of Positive Lgb Identity., Ghazel Tellawi

Electronic Theses and Dissertations

The purpose of the current study was to examine LGB identity from a multidimensional approach in the context of microaggressions. The aims of the study were as follows: 1) to examine whether positive and negative facets of LGB identities are correlated; 2) to determine whether positive LGB identity facets served as protective factors against the negative impact of microaggressions; 3) to explore the unique contribution of having a positive LGB identity against the negative impact of microaggressions when compared to other protective factors (social support and outness). Participants were 135 undergraduate students recruited through the University of Louisville’s research participant …

Sexual Violence And The Institutional Integration Of First Year College Students., Ann Katherine James

Electronic Theses and Dissertations

This purpose of this study is to explore the relationships among identity, sexual violence, reporting choices, perception of campus climate and institutional integration. Student affairs professionals and other educators are particularly concerned about the effects of sexual violence on college students because of the deleterious effects that such violence has on factors critical to student success. Research indicates that 1 in 5 students will experience actual or attempted sexual assault while in college (Fisher, 2000). Students with greater levels of social and academic integration have a greater commitment to their college or university and thus graduate at higher rates (Tinto, …

The Unequal City: The Mass Criminalization Of The Urban Poor., Elizabeth Michele Jones

Electronic Theses and Dissertations

Exploring and understanding the widespread use of arrests and incarceration in urban neighborhoods of concentrated poverty is the subject of this dissertation. The research addresses gaps in theoretical debates about the causes of mass criminalization that position the phenomenon as the result of either neoliberalism or racism. In addition, the dissertation explores the impact of mass criminalization on urban citizenship. Urban citizenship is a theoretical frame that considers the substance of the economic, social, political, and mobility dimensions of city life. The research methodology is a case study of two impoverished neighborhoods in the city of Louisville, Kentucky that incorporates …

"Why Am I In School?": A Mixed Methods Investigation Into Stopping Out Of College., Kathryn Gardner Adamchik

Electronic Theses and Dissertations

Higher education research often looks at student retention as a dichotomous outcome, either students stay enrolled or not; however, students’ enrollment pathways are more complicated than that as they frequently transfer, swirl between institutions, and stopout and return. This study was designed to explore students who stopout and return to the same institution within their six-year graduation window. More specifically, I sought to learn who stops out, why they stopout, what happens while out, why they come back, and what may be different upon their return. Four conceptual frameworks were applied to understand students’ experiences with stopping out including Tinto’s …

Influences Of Porphyromonas Gingivalis On The Viability Of Esophageal Cancer Cells., Muddasir Mohammed

Electronic Theses and Dissertations

A recent study demonstrated an association between the Porphyromonas gingivalis (Pg)infection and the progression of ESCC (Gao et al., 2016). However, how Pg infection affects the nature of ESCC remains unknown. We examined the effects of Pg on the proliferation and chemotherapy drug-induced apoptosis of ESCC cells and elucidated the signaling molecules involved in these processes. Method: Apoptosis and cell proliferation were tested using Flow cytometry and Tetrazolium salt based proliferation assays respectively. Western Blot was utilized to detect the protein expression. CDK2 and Cyclin E were knocked down in the ESCC cells through transfection. Unpaired T-test and …

Exploring The Capabilities Approach In A Sport For Development And Peace Setting., Jeffrey F. Levine

Electronic Theses and Dissertations

Sport for Development and Peace (SDP) has become a popular approach to development. However, critical scholars remain skeptical of SDP’s effectiveness. Utilizing a holistic approach that includes local voices may help a program’s effectiveness. Scholars are searching for a framework flexible enough to accommodate the varied nature of programs. One theoretical approach is the Capabilities Approach, which evaluates well-being based on what people can do and be. This case study explored the Capabilities Approach in an SDP setting. Youth Odyssey, a non-profit organization that works with at-risk youth through adventure programming, was the case. This case study explored what role …

Counter Narratives: A Phenomenological Study Of High Achieving African American Male High School Students., De'nay Lanise Speaks

Electronic Theses and Dissertations

This dissertation examined the contributing factors to the success of high achieving African American male high school students who attend an all-male, predominantly Black, college-preparatory academy. The study took place in an urban, Midwestern school district and highlighted the lived experience and perspectives of students who have at least a 3.0 grade point average. Through the lens of Critical Race Theory, this study was an effort to bring attention to counter narratives to the otherwise deleterious image portrayed of African American males. The history and plight of the African American male is included to provide a comprehensive understanding of the …

Understanding The Relationship Between Anti-Transgender Bias, Non-Affirmation, And Post-Traumatic Stress: A Model Of Internalized Transphobia-Mediated Post-Traumatic Stress., Sebastian Mitchell Barr

Electronic Theses and Dissertations

Transgender people’s increased risk for negative mental health outcomes, when compared to cisgender peers or the general public, has been well documented in the psychological literature. Researchers have begun to establish empirical support for a relationship between anti-transgender bias, non-affirmation of gender identity, internalized transphobia, and other transgender-specific minority stressors. Although little work has explored the mechanisms of this relationship, some psychologists have proposed conceptualizing these factors as potentially traumatic experiences and understanding the poor mental health outcomes as manifestations of complex post-traumatic stress. In this dissertation, I examine whether there is empirical support for this framework by evaluating the …

Arab-Islamic And Folk Health Models: New Perspectives On Syrian Refugee Resettlement In The Us., Ghina Kheir

Electronic Theses and Dissertations

This research builds upon the framework of the Health Traditions Model (HTM) as described in 1994 by Rachel Spector, a scholar and practitioner of a cultural care model of health among multicultural populations. I integrate this model into a study of Syrian refugees’ resettlement experience in Louisville, Kentucky. Syrian refugees’ voices and narratives of their corresponding Arab-Islamic and folk approaches to health, illness, and healing are the launch site for this project. I argue that approaching the resettlement experience from the refugees’ own cultural and Arab-Islamic perspectives toward health, illness, and healing aids in achieving a pluralistic health care system …

Direct And Indirect Effects Of Invasive Cirsium Arvense On Pollination In Southern Appalachian Floral Communities, Jesse Daniels

Electronic Theses and Dissertations

Invasive plants can alter pollination dynamics in invaded communities by disrupting patterns of pollinator visitation, pollen transfer dynamics (conspecific [CP] and heterospecific [HP]), and reproductive success. The direction of invasive effects (competitive, neutral, and facilitative) may be partially determined by spatial scale and species’ floral traits. Here, we investigated pollinator visitation, CP and HP receipt, and pollen tube growth for species in a C. arvense present community and non-present community at two scales. At the community-level, the effect of C. arvense on pollinator visitation varied among species. Floral symmetry seemed to explain this variation. At the floral neighborhood-level, we found …

Localization Of Sip470, A Plant Lipid Transfer Protein In Nicotiana Tabacum, Shantaya Andrews

Electronic Theses and Dissertations

SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed …

Characterization Of Microreactor And Analysis Of Carbonyl Compounds In Exhaled Breath., Qi Li

Electronic Theses and Dissertations

Analysis of trace volatile organic compounds at parts per billion (ppbv) to parts per trillion (pptv) level has become an important research frontier because of the applicability for environmental monitoring and noninvasive diagnosis of diseases. The methods of preconcentration for detection of trace aldehydes and ketones both in ambient air and human exhaled breath have increased considerably over the last decade. However, the majority of these methods are not efficient. In this dissertation, we have improved an innovative microreactor that is suitable for quantitative analysis of volatile carbonyl compounds (VOCs) in ambient air as well as in human exhaled breath. …

Evaluation Of The Predictive Ability Of A Screening Instrument Used To Diagnose Co-Occurrence Of Anxiety In Children With High-Functioning Autism., Austin Zachary

Electronic Theses and Dissertations

Anxiety has shown to have a high co-occurrence in children with Autism Spectrum Disorder (ASD), but has been difficult to co-diagnose. This study analyzed the ability of the Screen for Child Anxiety-Related Emotional Disorders (SCARED) to predict positive ASD screenings of the Social Responsiveness Scale (SRS). Parents and children ages 8 to 18 years (N = 100) were screened for anxiety using the SCARED and autism using the SRS. Moderate correlation was found between parent-reported SCARED and positive screening of the SRS (r = .579), with weak correlation found in the child-reported SCARED (r = .105). LASSO regression was conducted …

Effect Of Grape Components On Periodontal Disease., Juili Subodh Shivde

Electronic Theses and Dissertations

Background: Half of the adult American population (47 %) are victims of periodontitis, a chronic inflammatory disease which leads to destruction of the tissues supporting the tooth. Periodontitis is initiated by periodontal pathogens and caused by excessive or prolonged host mediated inflammation. Porphyromonas gingivalisis one important agent implicated in the etiopathogenesis of chronic periodontitis. Pro-inflammatory cytokines induced by periodontal pathogens can cause alveolar bone loss and periodontitis. Bone loss is caused by the imbalance of bone formation and bone resorption. Osteoclasts are major cells that cause bone resorption. Moreover, periodontal inflammation also leads to bone resorption. Grapes are rich …

Adolescent Sexual And Reproductive Health In The Gambia., Sandhya Lohani

Electronic Theses and Dissertations

Adolescence is a crucial period in human development. Knowledge and awareness about when to have children, when to have sex, and use of contraceptives impacts the chances of teenage pregnancy and risk of sexually transmitted diseases. The objective of this study is to determine the prevalence and associated risk factors related to age of sexual debut, teenage pregnancy, and use of contraceptives in the Gambia. This study also compares the data with 2014 survey research conducted in the Gambia. The total study population were 50 adolescents aged 13-21 years from Bafuluto and surrounding communities in the Gambia. Among 50 respondents, …

The Role Of Aldehyde Dehygrogenase 2 In Liver Injury Caused By Vinyl Chloride And High-Fat Diet., Liya Chen

Electronic Theses and Dissertations

Background. Vinyl chloride (VC) is an abundant environmental contaminant that causes steatohepatitis at high exposure levels. We have shown previously that low concentrations of VC exacerbate high fat diet (HFD)-induced liver injury in mice. The mechanisms involved in the progression of liver injury caused by VC and HFD include oxidative stress, inflammation, metabolic and mitochondrial dysfunction. Mitochondrial aldehyde dehydrogenase 2 (ALDH2) serves as a key line of defense against most reactive aldehydes, including lipid aldehydes (e.g., 4-HNE) and chloroacetaldehyde (VC metabolite). We hypothesize that this defense may play a key role in the interaction between HFD and VC in fatty …

Epidermal Growth Factor-Like Ligands Regulate Dimer Selection., Jamie S. Rush

Electronic Theses and Dissertations

There are thirteen known endogenous EGF-like ligands. We previously reported that Betacellulin (BTC) increases ligand-mediated corneal wound healing more than Epidermal Growth Factor (EGF) [Peterson et al. (2014) IOVS 55(5):2870-80], although the molecular reason for this is unknown. Despite being better at promoting wound healing via enhanced cell migration, BTC has reduced receptor affinity and weaker induction of EGFR phosphorylation. These data indicate that BTC’s response is not due to enhanced affinity or EGFR-kinase activity. Receptor phosphorylation and proximity ligation assays indicate that BTC treatment significantly increases ErbB3 phosphorylation and EGFR:ErbB3 heterodimers. BTC traffics EGFR at a faster rate than …

Synthesis And Characterization Of Targeted Bar Encapsulated Polylactic-Co-Glycolic Acid Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Ranjith Radha Krishnan

Electronic Theses and Dissertations

Periodontal disease is one of the most prevalent infectious diseases worldwide. Between 30-50% of the global adult population suffers from periodontal disease. Some form of periodontitis is present in 46% of American adults, corresponding to annual expenditures in excess of 14 billion dollars for treatment and prevention. Current treatments for periodontal diseases involve mechanical removal of plaque, correction of risk factors, gingival surgery and/or antibiotic therapy. To our knowledge there is no effective therapeutic approach that aims to limit pathogen colonization of the oral biofilm or re-colonization after treatment. Interaction of the pathogen Porphyromonas gingivalis with oral streptococci is critical …

Particulate Hexavalent Chromium Does Not Induce Centrosome Amplification In Sperm Whale And Bowhead Whale Cells., Jennifer Haruka Toyoda

Electronic Theses and Dissertations

Hexavalent chromium [Cr(VI)] is a widespread environmental and occupational carcinogen. The mechanism of carcinogenesis remains poorly understood, but chromosome instability (CIN) is the dominant theory. How numerical CIN arises is unclear, but it correlates with centrosome amplification. Both phenotypes are hallmarks of cancers, early events in carcinogenesis, and have been shown to occur after Cr(VI) exposure to human skin and lung fibroblasts. In this study, we investigate numerical CIN and centrosome amplification in whale cells. Whales are our closest marine relatives, have long lifespans, and are exposed to environmental Cr(VI). Importantly, they have low cancer rates and cell culture studies …

Early-Childhood Computer-Based Testing: Effects Of A Digital Literacy Intervention On Student Confidence And Performance, Benjamin-David Christopher Legrand

Electronic Theses and Dissertations

Early-childhood digital students grow up in a fast-evolving age of technology requiring them to use and create with technologies and demonstrate core content knowledge. Although third grade students are mandated to master a new language of standardized testing, a large percentage must also learn a language of technology to complete new computer-based tests to measure content mastery. Krashen (1982) defines high affective filter as negative emotional/motivational factors interfering with understanding and cognition. This high affective filter reduces confidence and negatively impacts measuring content mastery on new computerbased tests. Two third grade classrooms at a high-poverty metropolitan school participated in a …

The Impact Of Foreign Aid On Extreme Poverty: A Case Study Of Liberia’S Development Complexities (1980-2018), Roosevelt Seedee

Electronic Theses and Dissertations

Many countries in West Africa, including Liberia, remain trapped in extreme poverty and dysfunctional social services amid continued increase in foreign aid. This study examines complexities influencing decisions of government donors in determining the kind of development assistance needed in Liberia and nation states in West Africa. This research explains the ways in which aid perpetuates poverty instead of alleviating it using interdisciplinary research approaches. Although aid is critical to Liberia’s development agenda, aid implementation faces numerous challenges because of extant poverty and rampant corruption.

Part of the problem in Liberia is not solely the failure of aid to meet …

The Role Of Cyp2a5 In Cadmium-Induced Liver Injury, Julia Salamat

Electronic Theses and Dissertations

Cadmium is present in food and groundwater. Tobacco smoking and occupational exposure are also major sources for cadmium. Cadmium is primarily accumulated in liver, a major organ metabolizing exogenous chemicals. Chemical metabolism may cause detoxification, but it can also cause bio-activation resulting in liver damage. Cytochrome P450s (CYP) are major liver metabolism enzymes, and cadmium chloride (CdCl2) can induce CYP2A5 in mice. We examined the effect of CYP2A5 on CdCl2-induced liver injury using CYP2A5-knockout (cyp2a5-/-) mice. The cyp2a5-/- mice and their control WT mice were injected CdCl2 intraperitoneally at 5 mg/kg body weight, respectively, to induce liver injury. …

Perceptions Of Confidentiality And Stigma Associated With Use Of Counseling Services, Jesi Hall

Electronic Theses and Dissertations

Counseling services are offered in most universities, but are often underutilized. Past researchers found that students listed time, cost, stigma, and confidentiality concerns as their top reasons for avoiding seeking help (e.g., Clement et al., 2015; Dearing, Maddux, and Tangney, 2005). The purpose of this study was to identify whether concerns about confidentiality affect stigma related to use of mental health services. Students answered questions about perceived stigma and the reasons they have avoided seeking counseling in the past. Those who had previously used services reported fewer confidentiality concerns. In a stepwise multiple regression, concerns about confidentiality were found to …

Imidacloprid Persistence, Mobility, And Effect On Ecosystem Function, Joanna Hardin

Electronic Theses and Dissertations

Imidacloprid is a neonicotinoid pesticide used to protect against biting and sucking insects. Land managers rely on its systemic properties, however long-term studies investigating imidacloprid effects on ecosystem function are limited. This study investigated imidacloprid applications to Tsuga caroliniana and Tsuga canadensis over time and compared concentrations to measures of ecosystem function including soil respiration, microbial function, and invertebrate density. Results indicate that imidacloprid is persistent (p0.5). Microbial function and invertebrate density were not significantly different between control and treatment locations nor did imidacloprid concentrations correlate with ecosystem functional indicator activity (p>0.05). It is evident that imidacloprid does not …