Digital Commons Network^™

(Pseudo)Phase Tuning In The Service Of Synthesis: Technologies For Sustainable Catalysis., Justin Douglas Smith

Electronic Theses and Dissertations

Strategies for manipulation of chemical phases were explored with emphasis on synthetic chemistry applications. Some strategies are fairly divergent from their origin, but all ultimately began from investigations of micellar media. Accordingly, Chapter 1 reviews the historical development of micellar catalytic processes. Chapters 2 and 3 detail development of synthetic methods in micellar solutions of designer surfactant PS-750-M. PS-750-M was designed to create benign micellar solutions which effectively mimic toxic organic solvents; it contains a proline-based linker between its hydrophobic and hydrophilic regions which imparts increased polarity to the micellar core. Chapter 2 details an interfacial nucleophilic substitution of perfluoroarenes …

Peer Verbal Sexual Harassment In Early Adolescence: A Feminist And Sexual Scripts Theoretical Approach., Shawn M. Rolfe

Electronic Theses and Dissertations

Until recently, sexual harassment research has focused primarily on adults and college and high school students with little attention given to younger students. While recent research has noted sexual harassment occurs among younger students, the literature addressing sexual harassment among middle school students is still sparse. Additionally, most studies of sexual harassment have focused on sexual harassment generally, with no distinction made between verbal and sexual harassment. In fact, only limited research has been exclusively conducted on verbal sexual harassment. Using self-report data, the current study adds to the literature by examining the nature of verbal sexual harassment as well …

Electrochemically-Modulated Surface Plasmon Waves At A Nano-Scale For Investigations And Applications Of Redox Assemblies., Aymen Hasan Qatamin

Electronic Theses and Dissertations

In this thesis, an electrochemically-modulated surface plasmon resonance (EC-SPR) technique was developed for challenging investigations and applications in different redox assemblies. The principle of the technique is based on the nano-scale confined and sensitive interplay between a surface plasmon wave and a redox-active probe featuring an optical transition when undergoing a faradaic redox process. Such faradaic process is electrochemically controlled by modulating the surface electric potential of the SPR platform to create an optical output signal that is substantially immune to the negative impacts of background environment and enabling the identification and quantification minute properties linked to a molecular event …

Soluble Epoxide Hydrolase Inhibition: A Novel Therapeutic Strategy In Alcoholic Liver Disease., Jeffrey Barr Warner

Electronic Theses and Dissertations

Emerging evidence suggests that soluble epoxide hydrolase (sEH) inhibition is a valuable therapeutic strategy for the treatment of numerous diseases. sEH rapidly degrades cytochrome P450-produced epoxygenated lipids (epoxy-fatty acids, epFAs), which generally exert beneficial effects on several cellular processes. sEH hydrolysis of epoxy-fatty acids produces dihydroxy-fatty acids which are typically less biologically active than their parent epoxide. sEH inhibition has shown efficacy in treating numerous pathologies associated with non-alcoholic liver disease, yet no efforts to date have tested the efficacy of sEH inhibition in alcoholic liver disease (ALD). Herein, we measured the levels of sEH expression and epFAs in human …

Nonlinear Control And Observation Of Full-Variable Speed Wind Turbine Systems., Nicholas Hawkins

Electronic Theses and Dissertations

With increasing concern for the environmental effects of power generation from fossil fuels, wind energy is a competitive source for electrical power with higher efficiency than other clean sources. However, the nature of this power source makes controlling wind turbines difficult. The variability of wind as a source either requires highly accurate measurement equipment or sophisticated mathematical alternatives. In addition to the unknown quantities of the weather itself, the efficiency of power capture at the turbine blades is highly nonlinear in nature and difficult to ascertain. The ability of either determine these troublesome quantities, or control the system despite ignorance …

Trust In Primary Care Providers: Perspectives From Deaf People., Greir Ander Huck Flynn

Electronic Theses and Dissertations

Trust is an integral part of the patient-provider relationship, responsible for influencing several key aspects of healthcare behaviors and patient perceptions of health outcomes. Though the topic has previously been discussed by scholars, little evidence exists to support the application of our understanding of trust in culturally Deaf populations. As such, this dissertation seeks to lay the necessary groundwork for further investigations on the topic by first understanding the nature of trust in patient-provider relationships from the perspectives of culturally Deaf people. The dissertation is comprised of five chapters. The first chapter provides an introduction to salient terms and concepts …

A Novel Role For Rassf1a In The Regulation Of Ras Activation., Desmond Ramón Harrell Stewart

Electronic Theses and Dissertations

Ras is the most frequently activated oncogene in human cancer. It is not only the most frequently mutated oncogene, but is also rendered hyperactive in the wild-type form by aberrant regulation. Ras drives transformation and contributes to tumor aggressiveness by activating multiple downstream mitogenic effectors. Ras also possesses the paradoxical ability to induce apoptosis and senescence. Ras-induced apoptosis is not well understood, but has been largely attributed to the RASSF tumor suppressors, particularly RASSF1A. RASSF1A mediates Ras-induced apoptosis by activating pro-apoptotic proteins such as the MST kinases and BAX. RASSF1A is among the most frequently inactivated tumor suppressors in human …

Epithelial Responses To Porphyromonas Gingivalis In The Community Context., Zackary Ray Fitzsimonds

Electronic Theses and Dissertations

Porphyromonas gingivalis, a keystone pathogen for periodontal disease, initiates a complex molecular dialogue with gingival epithelial cells, leading ultimately to disruption of host signaling pathways. Dysbiotic host responses are also thought to contribute to the initiation and progression of oral squamous cell carcinomas. Recently, the homeostatic commensal Streptococcus gordonii has been shown to antagonize P. gingivalis-induced epithelial cell signaling events in host cells, such as proliferation and migration. The aim of this study was to characterize pathways that P. gingivalis targets to disturb host signaling, with a specific focus on pathways that S. gordonii can restore to homeostatic …

Incarceration Experiences Of Older African American Adults Living With Hiv - A Constructivist Grounded Theory Study., Verena Schmidt

Electronic Theses and Dissertations

Two epidemics disproportionally impact older (50 years and over) African Americans compared to the general U.S. population: Incarceration and HIV/AIDS. Those aged 50 and older constitute the fastest growing age group of persons who are incarcerated in the United States. It is estimated that by the year 2030 about one third of the incarcerated population in the U.S. will be aged 55 years and older. While areas of “Incarceration, racial disparities and HIV” as well as “Aging and HIV” have been well studied and discussed in the literature, little is known about the lived incarceration experiences of African American persons …

Compassion Or Neoliberal Governance: Critiquing The Discourse Of Compassionate Louisville., Ekramul Islam

Electronic Theses and Dissertations

Since 2011, the city of Louisville, the site of this research, has been calling itself “Compassionate City.” The dissertation takes Compassionate Louisville as a city brand and seeks to investigate the effect of the image construction on the local politics. It aims to investigate the latent discourses produced by Compassionate Louisville in relation to the neoliberal political economy of the city. This dissertation employs the Critical Discourse Analysis (CDA) methodology. Compassionate Louisville, being a brand, produces a discourse of compassion. The CDA methodological approach helps to investigate this manufactured discourse in relation to the neoliberal socio-political context of the city. …

Pentecostal Women And Religious Reformation In The Progressive Era: The Political Novelty Of Women’S Religious And Organizational Leadership, Sherry Kaye Ms.

Electronic Theses and Dissertations

The Progressive Era in America from 1870 to 1920 introduced unprecedented change in the way Americans lived, worked, and thought about themselves in relation to the rest of the world. New platforms of charitable benevolence, religious activism, and legislative reform were enacted to meet the changed demographic landscape initiated by waves of new immigration from Europe. The tenor of religious worship shifted in mainstream and evangelical churches to reflect not only new ways of response to these changes, but new ideas of women as authoritative leaders in secular and religious institutions. Charismatic evangelical women influenced by an era of change …

Serious Mental Illness And Rural Primary Care: Provider Training, Attitudes, And Opinions, Lydia Eisenbrandt

Electronic Theses and Dissertations

Healthcare resources are especially limited in rural regions of the US. The lack of Primary Care Providers (PCPs) and mental/behavioral health services is problematic, as there are high rates of behavioral and medical concerns within rural populations. Special populations, such as rural persons with Serious Mental Illness (SMI), are medically complex and represent a vulnerable and underserved population. Healthcare outcomes for persons with SMI are poor compared to the general population and commonly lead to premature death. Various barriers prevent this population from accessing optimal healthcare, especially in rural areas, due to negative perceptions/stigma, a lack of understanding from PCPs, …

Methods For Mass Data Acquisition Of Whiskey Webs., Adam D. Carrithers

Electronic Theses and Dissertations

Whiskey webs are a recently discovered phenomenon in which the monolayer collapse during evaporation of diluted American whiskey results in self-assembled, web-like structures. It was found that the pattern of weblike structures were unique between different American whiskey products. It is desirable to use these unique patterns as a “fingerprint” which can be used to identify whiskeys, their chemical constituents, and perhaps even counterfeits. In pursuit of identifying whiskey products based on their web pattern, it is necessary to have a large database of samples imaged for each product. The device discussed herein is the realization of an apparatus capable …

Development, Expansion And Role Of Myeloid-Derived Suppressor Cells In Post-Sepsis Immune Suppression, Tuqa Alkhateeb

Electronic Theses and Dissertations

Myeloid-derived suppressor cells (MDSCs) numbers increase significantly in sepsis and are associated with high mortality rates. These myeloid cell precursors promote immunosuppression, especially in the late (post sepsis) stage. However, the mechanisms that underlie MDSC expansion and programming are not completely understood. To investigate these mechanisms, we used a cecal-ligation and puncture (CLP) mouse model of polymicrobial sepsis that progresses from an early/acute proinflammatory phase to a late/chronic immunosuppressive phase. Previous studies in our laboratory showed that microRNA (miR)-21 and miR-181b elevate levels of the transcription factor nuclear factor 1 (NFI-A) that promotes MDSC expansion. We report here that miR-21 …

Utilizing The Public On Public Lands: The Application Of Community Science To Monitor And Model Erosion In National Forests, Jacob L. Hansen

Electronic Theses and Dissertations

Unpaved forest roads are adversely affecting coldwater streams through excessive erosion and the subsequent sedimentation of adjacent waterways. To help identify areas of concern, Trout Unlimited (TU) in the Southern Appalachian region developed a Community Science initiative to gather data on sediment sources and stream-road crossings. Volunteers were recruited and trained to monitor road and trail conditions and collect and submit data using a Survey123 application on their cell phones. Analysis of the contributed data reveals statistical connections between drainage type and both erosion level and stream sedimentation. The contributed data were also included as a calibration for the lite …

The Relationship Between Cell-Free Dna And Resistance Training, Henry Lang

Electronic Theses and Dissertations

The primary purposes of this dissertation were to explore relationship between cell free DNA (cf-DNA), creatine kinase (CK), C-reactive protein (CRP), vertical jump testing delayed onset muscle soreness (DOMS) in response to a high-volume resistance training protocol, and to assess the sensitivity of cf-DNA to different resistance training volume loads. The secondary purpose was to examine the relationship between cf-DNA and relative strength. Study 1 was an exploratory attempt to discover relationships between cf-DNA, CK, CRP, delayed onset muscle soreness, and performance variables. Seventeen resistance trained males were recruited, 9 were randomly assigned to receive BCAAs while 8 received a …

The Effects Of Elevation On Foraging Behavior Of Bats In Southern Appalachia, Victoria Long

Electronic Theses and Dissertations

There are limited studies on bat activity in higher elevations in the Appalachian region. Levels of bat activity were compared in south central Appalachia at low (< 914.4 m) and high (> 1, 524 m) elevations in open, forest edge, and riparian habitats. Additionally, habitat suitability was modeled for a common species, big brown bat (Eptesicus fuscus). The study started May 27^th 2019, and sites were monitored biweekly until October 2^nd 2019. Six species and one genus were recorded during the study. Species from the Myotis genus were grouped together because of similar call characteristics. Results show that species were significantly more …

The Effects Of Increasing Running Speed On Vgrf And Asymmetry, Kaela M. Hierholzer

Electronic Theses and Dissertations

Biomechanical and physiological parameters related to running performance are usually studied separately. However, evaluating both aspects together could be beneficial in improving athletic performance. The purpose of this study was to observe the change in peak vGRF and asymmetry as speed increases, while observing physiological responses during a O_2maxtest. Data from athlete monitoring of 12 cross-country and triathlon athletes were analyzed. The athlete monitoring protocol included three unweighted countermovement jumps and a O_2maxtest performed by the athletes. The athletes had an average O_2maxof 53.4 ± 7.7 mL/kg/min, while their average vGRF asymmetry throughout the …

Tumor-Derived Exosomes Drive Immunosuppressive Macrophages In A Pre-Metastatic Niche Through Nf-Kβ Dependent Glycolytic Metabolic Reprogramming., Samantha M. Morrissey

Electronic Theses and Dissertations

The formation of a pre-metastatic niche is a fundamental requirement for primary tumor metastasis. One of the defining characteristics of a pre-metastatic niche is infiltration of immunosuppressive macrophages. However, how these macrophages acquire their immunosuppressive phenotype remains largely unexplored. Here, we demonstrate that tumor-derived exosomes (TDE) polarize macrophages towards an immunosuppressive phenotype characterized by increased PD-L1 expression through NF-kB-dependent metabolic reprogramming in mice and humans. While NF-κB has previously been shown to act as a direct transcription factor for PD-L1, we report a novel mechanism where TDE-induced NF-κB activation drives PD-L1 expression by augmenting the glycolytic capacity of macrophages through …

A Study Of The United States Influence On German Eugenics., Cameron Williams

Electronic Theses and Dissertations

This thesis is a study of the influence and effects that the United States had upon Germany from the rise of eugenics to its fall following the end of World War II. There are three stages to this study. First, I examine the rise of eugenics in the United States from its inception to the end of World War I and the influence it had upon Germany. Then I examine the interwar era along with the popularization of eugenics within both countries before concluding with the Second World War and post war era.

My thesis focuses on both the active …

Paleoenvironmental Reconstruction Of Quaternary Saltville, Virginia, Using Ostracode Autecology, Austin Gause

Electronic Theses and Dissertations

The Saltville valley in southwestern Virginia is home to Quaternary localities containing paleontological and archaeological remains. Historically the valley has been mined for salt and the small lakes, ponds and springs along the valley floor have a brackish signature. A preliminary report on the site’s ostracode fauna suggested that the site’s water was not always saline. This study analyzed modern and Quaternary ostracodes to understand the valley’s hydrologic and chemical evolution. Sediments contained primarily freshwater species, including the environmentally sensitive Candona crogmaniana. The presence of Pelocypris tuberculatum and a new Fabaeformiscandona species throughout a vertical section spanning the latest Pleistocene …

Investigation Of Factors Affecting The Hiv Continuum Of Care In Tennessee, Elaine Loudermilk

Electronic Theses and Dissertations

Introduction: Factors contributing to the HIV continuum of care (HCC) for adults in Tennessee (TN) have not been studied in depth with known predictors for HIV risk, specifically adverse childhood experiences (ACEs), among low annual income or sexual minority groups [lesbian, gay, bisexual, or transgender (LGBT)].

Methods: A mixed methods study design was used to assess factors related to the HCC. Quantitative analysis used the TN Behavioral Risk Factor Surveillance System data to examine at risk and ever tested for HIV, to (1) investigate ACEs among adults with low-income (<$25,000 annually) in 2016-2017 and (2) among LGBT in 2018. Descriptive statistics and multiple logistic regression (MLR) analysis stratified by TN grand division were performed. Qualitative analysis included 11 interviews with persons living with HIV (PLWH) receiving care at a local clinic to evaluate surveillance questions related to ACEs and the HCC to develop a culturally appropriate survey.

Results: Quantitative results found that among LGBT (N=262) and low-income …

Roles Of Endothelial Cell Heat Shock Protein A12b And Β-Glucan, A Reagent For Trained Immunity In The Regulation Of Inflammation In Sepsis, Fei Tu

Electronic Theses and Dissertations

Sepsis is dysregulated host immune response to infection causing life-threatening organ dysfunction. Endothelial cell dysfunction and uncontrolled inflammatory responses are two contributors for sepsis-induced mortality. The crosstalk between endothelial and immune cells plays a critical role in the pathophysiology of sepsis. Therefore, understanding the mechanism of interaction between endothelial and immune cells will provide novel information to develop therapeutic strategies for sepsis.

Pathogen associated moleculear patterns (PAMPs) and/or damage associated molecular patterns (DAMPs) produced during sepsis, activate endothelial cells to increase the expression of adhesion molecules, attracting immune cell infiltration into the tissues. Uncontrolled inflammatory responses during the early phase …

Thwarted Interpersonal Needs, Depression, And Sleep Disturbances In Primary Care: Does Gratitude Help You Sleep?, Heather R. Altier

Electronic Theses and Dissertations

Sleep disturbances are prevalent in primary care patients and can be exacerbated by interpersonal dysfunction and depression. As well, thwarted interpersonal needs (TIN), including thwarted belongingness and perceived burdensomeness, contribute to depression. However, the presence of gratitude, a cognitive-emotional protective factor, may improve symptoms. We longitudinally examined the mediating role of depressive symptoms on the relation between TIN and sleep disturbances, and the moderating role of gratitude on the TIN-sleep disturbances and depression-sleep disturbances linkages. Our primary care patient sample (N = 223) completed self-report surveys at baseline (T1) and at a one-year follow-up (T2; n = 97). Patients …

Examining The Relationship Among Middle School Students’ Performance On The Tnready Assessment, District Checkpoints, And Teacher-Assigned Grades, Kristina Dempsey

Electronic Theses and Dissertations

The purpose of this nonexperimental, quantitative study was to determine if there is a significant correlation among teacher-assigned grades (TAG), district checkpoint scores (CP), and student scale-scores on TNReady tests. The focus was on 1,445 seventh and eighth grade students who were enrolled at a middle school in northeast Tennessee during the academic years of 2017-2018 and 2018-2019, specifically for the content areas of English Language Arts and mathematics. The second purpose of this study was to examine any moderating effects of the categorical variable, students with disabilities (SWD) status, on the correlations among the district and state assessments and …

Examining The Warm Handoff In Rural Integrated Care, Jamie Tedder

Electronic Theses and Dissertations

An ever-growing body of evidence supports the efficacy of integrated care as a treatment approach; however, less is known about what specific components of integrated care are most effective. This is especially true of warm handoffs, which are an often discussed but understudied process in integrated care. A total of 246 patient charts were reviewed to determine if type of referral (warm handoff or traditional) increased the likelihood of follow-up with behavioral health services as well as factors that might impact this relationship. There were no significant differences between type of referral and likelihood of follow-up with behavioral health services. …

Axonal Regrowth Of Olfactory Sensory Neurons After Chemical Ablation And Removal Of Axonal Debris By Microglia, Rudy Chapman

Electronic Theses and Dissertations

Olfactory sensory neurons (OSNs) are contained within the olfactory epithelium (OE) and are responsible for detecting odorant molecules in the air. The exposure of OSNs to the external environment is necessary for their function, but it also leaves them exposed to potentially harmful elements and thus results in a high turnover rate. Despite the high turnover, the olfactory sense is maintained throughout life through the division of a population of stem cells that produce new OSNs both during normal turnover and after an injury occurs in the OE. When new OSNs are born, they must extend axons from the OE …

Head Start Preschool Teachers’ Perceptions Of Reggio Emilia Principles Practiced Within Their Own Setting: A Case Study, Tara Terry Voit

Electronic Theses and Dissertations

The Reggio Emilia Approach (REA) and Head Start (HS) value high-quality early education, support for families, and community partnerships according to the literature. Exploring principles of REA in relation to the HS program model may reveal alignment that will inform high-quality developmentally appropriate practices that are meaningful and enhance learning outcomes. This multiple case study explores the REA in alignment within three Head Start programs in New Mexico, Massachusetts, and Tennessee. Participant groups include one education director, one education leader, and four teachers in each of the three locations. In New Mexico, the HS program was located on a Native …

A Case Study Of Civil War Environmental And Medical History Through The Disease Seasoning Of The 58th North Carolina Infantry Regiment In East Tennessee, Ashlie Richard

Electronic Theses and Dissertations

This thesis is a case study aimed at a key argument in the emerging field of Civil War medical and environmental history. While historians have long acknowledged disease as a major killer during the Civil War, only recently have environmental and medical historians turned their collective attentions to unpacking the complex interconnections of disease, environmental conditions, and culture. By examining the 58^th North Carolina Infantry Regiment from the mountains of western North Carolina, this thesis asserts that the combined role of the disease environment and conditions in military camps created the massive outbreaks of disease that characterized the seasoning …

The Lived Experiences Of Adolescent Ell Students In East Tennessee, Sarah Mould

Electronic Theses and Dissertations

Adolescent immigrant students face many challenges upon entering public schools for the first time, especially in rural areas where schools may not have the resources and cultural competence to meet their needs. Background factors like culture, previous schooling, and socioeconomic status combine with contextual factors in the learning environment, which further affect their academic outcome. The qualitative tradition of phenomenology was used to explore and describe the experiences and challenges of thirteen Spanish-speaking immigrant or newcomer youth who entered East Tennessee public middle or high schools within the last fifteen school years.

The findings indicated that parents’ perspectives and understanding …