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Electronic Theses and Dissertations

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Does Speech-To-Text Assistive Technology Paired With Graphic Organizers Improve The Written Expression Of Students With Traumatic Brain Injuries?, Kayla Cuifolo Dec 2021

Does Speech-To-Text Assistive Technology Paired With Graphic Organizers Improve The Written Expression Of Students With Traumatic Brain Injuries?, Kayla Cuifolo

Electronic Theses and Dissertations

A traumatic brain injury (TBI) can range from mild to severe and can cause debilitating outcomes that require children to need specialized medical or educational services post-injury. Outcomes vary and are dependent on the location of injury, age, severity, and environmental factors. Some common deficits that happen as a result of a brain injury are fine motor and executive functioning skill difficulties. Fine motor and executive functioning skills are an important component of written expression. Therefore, this current study utilized a brief experimental analysis in order to determine the effects that speech-to-text assistive technology along with a graphic organizer has …


The Lived Experiences Of Self-Concealment Among Lgbq+ Counselors In The Workplace, Richard Charette Ii Dec 2021

The Lived Experiences Of Self-Concealment Among Lgbq+ Counselors In The Workplace, Richard Charette Ii

Electronic Theses and Dissertations

This study sought to unearth the narratives of LGBQ+ counselors’ experiences of self-concealment in the workplace. Self-concealment was an identity management strategy that was defined as an active, and sometimes persistent effort to conceal LGBQ+ identity. Self-concealment of affectional identity was well researched in workplace contexts, however only one study existing study examined self-concealment among LGBQ+ counselors. In that previous qualitative inquiry, Jeffery and Tweed (2014) called for further exploration of self-concealment among LGBQ+ counselors when self-concealment emerged as a surprising finding.

The phenomenon of self-concealment was under-researched in counseling but has been extensively conceptualized as both a developmental stage …


Loneliness, Interpersonal Goals, Life Satisfaction, And Subjective Well-Being In Older Adults, Francesca C. Ezeokonkwo Dec 2021

Loneliness, Interpersonal Goals, Life Satisfaction, And Subjective Well-Being In Older Adults, Francesca C. Ezeokonkwo

Electronic Theses and Dissertations

Background: Loneliness is detrimental to health and is linked to numerous physiological and psychological problems. People across the age spectrum can be affected by loneliness at one point or another in their lives; however, older adults are disproportionally affected.

Aims: This study investigated the effect of interpersonal goals (compassionate and self-image goals), life satisfaction, and subjective well-being on loneliness in older adults and the influence of demographics and social support. The Ecosystem-Egosystem Theory of Social Motivation served as the theoretical framework.

Design and Method: This descriptive cross-sectional correlational study used the 2016 Health and Retirement Study. Participants were 65 years …


A Public Health Ethics Approach To Substance Use Disorder, Adele Flaherty Dec 2021

A Public Health Ethics Approach To Substance Use Disorder, Adele Flaherty

Electronic Theses and Dissertations

The goal of the dissertation is to undertake an analysis of substance use disorders that focuses on a public health ethics perspective. The ethical argument focuses upon justifying the use of harm reduction and is primarily concerned with the current opioid crisis. While substance abuse/misuse over the course of history has been identified as a public health concern, this dissertation presents substance use disorders over the course of the lifespan, examining various contexts in which it can affect daily living and health outcomes. It achieves this by analyzing substance use disorders through the lens of the socioecological model of public …


Examining The Interaction Of Adverse Childhood Events, Dental Issues, Asthma And Autism, Cathy Koetting Dec 2021

Examining The Interaction Of Adverse Childhood Events, Dental Issues, Asthma And Autism, Cathy Koetting

Electronic Theses and Dissertations

Dissertation supervised by Dr. Rebecca Kronk

Purpose: Adverse childhood events (ACEs) have a profound and long-term effect on the health outcomes of many children and adults. However, few studies have focused on specific ACEs and health outcomes in children with autism spectrum disorder (ASD). The aim of the study was to investigate if ASD moderated the relationship between ACEs and specific health outcomes in children who had been the subject of a child welfare system report.

Design and Methods: Using the second National Survey of Child and Adolescent Well-Being (NSCAW II), a secondary analysis was performed on a sample of …


Seasonal Flammability Comparisons Of Native And Exotic Plants In The Post Oak Savannah, Blackland Prairie, And Pineywoods Ecoregions Of Texas, Michael Tiller Dec 2021

Seasonal Flammability Comparisons Of Native And Exotic Plants In The Post Oak Savannah, Blackland Prairie, And Pineywoods Ecoregions Of Texas, Michael Tiller

Electronic Theses and Dissertations

East Texas’ diverse landscape can present year-round wildfire seasons that can be influenced by seasonal and regional differences in climate and physiography. Greater insight into the fundamental thermal behavior of wildland fuels can aid in fire behavior prediction and development of fire-resistant plant lists. This study focused on estimating seasonal and regional flammability characteristics of five evergreen species: yaupon (Ilex vomitoria), Chinese privet (Ligustrum sinense), greenbrier (Smilax spp.), eastern red cedar (Juniperus virginiana), and escarpment live oak (Quercus fusiformis); and two deciduous species: Chinese tallow (Triadica sebifera) and southern …


The Journey Towards Critical Self-Authorship For Native Lacrosse Athletes At Ncaa Division I Institutions., Brandon C. Joseph Dec 2021

The Journey Towards Critical Self-Authorship For Native Lacrosse Athletes At Ncaa Division I Institutions., Brandon C. Joseph

Electronic Theses and Dissertations

This dissertation is an examination of contemporary collegiate lacrosse as a symptom of settler colonialism and the experiences of Native lacrosse athletes at NCAA Division I historically white institutions. It begins with a brief historical overview of Native lacrosse and Native education through a lens of settler colonial theory and Tribal Critical Race Theory. It uses Critical Indigenous Research Methods to examine the experiences of contemporary Native collegiate lacrosse players and their journey towards critical self-authorship. The ultimate goal was to explore the extent to which Native lacrosse athletes felt they could show up as their authentic Native self in …


Translating Particulate Hexavalent Chomium-Induced Chromosome Instability, Loss Of Homologous Recombination Repair And Targeting Of Rad51 From Human Lung Fibroblasts To Human Bronchial Epithelial Cells., Idoia Meaza Isusi Dec 2021

Translating Particulate Hexavalent Chomium-Induced Chromosome Instability, Loss Of Homologous Recombination Repair And Targeting Of Rad51 From Human Lung Fibroblasts To Human Bronchial Epithelial Cells., Idoia Meaza Isusi

Electronic Theses and Dissertations

Particulate hexavalent chromium [Cr(VI)] is a well-established human lung carcinogen. RAD51, a key protein in homologous recombination repair pathway, is inhibited after prolonged exposure to Cr(VI), leading to an increase in chromosome instability after prolonged exposures in human lung fibroblasts. chromosome instability is the proposed driver of Cr(VI) carcinogenesis. Since tumors from chromate workers develop from epithelial cells, we sought to translate these findings from human bronchial fibroblasts to human bronchial epithelial cells. We hypothesized Cr(VI) inhibits RAD51 after prolonged exposure leading to an increase in chromosome instability in human bronchial epithelial cells (BEP2D). We characterized the cytotoxicity and measured …


Part-Time., Anthony Mark Piedmonte Dec 2021

Part-Time., Anthony Mark Piedmonte

Electronic Theses and Dissertations

This thesis is a creative writing project in prose covering the first 70 pages of a novel manuscript titled Part-time. Part-time will center around a 25-year-old medical school dropout starting a new job at a neighborhood golf clubhouse and gym. This coming-of-age narrative follows Danny as he struggles with his sexual identity, student debt, and job outlook after returning to the privileged community he grew up in. This sample will introduce the major characters, the clubhouse setting, and the forming relationship dynamics. By the end of these pages, readers will gain insight into how the part-time employees of the Longwood …


A Soft Place To Land: The Importance Of Otherparenting At Pwis., Stephanie Renae Mayberry Dec 2021

A Soft Place To Land: The Importance Of Otherparenting At Pwis., Stephanie Renae Mayberry

Electronic Theses and Dissertations

This phenomenological study examined the importance of otherparenting performed by Black student affairs professionals at a predominantly White research-intensive institution in the mid-South. Otherparenting is a U.S. tradition that has West African roots in chattel slavery. Otherparenting is defined as those that assist blood mothers and fathers by sharing parenting responsibilities. Semi-structured interviews were conducted with seven participants across various functional units at the institution. The examination illustrated the commitment Black staff have in assisting Black students through the college campus rife with structural barriers, while also accounting for the level of taxation the commitment to otherparenting places on Black …


Hospital-Based Professional Spiritual Care: Evaluating The Utilization, Availability, And Impact Of Chaplains., Kelsey B. White Dec 2021

Hospital-Based Professional Spiritual Care: Evaluating The Utilization, Availability, And Impact Of Chaplains., Kelsey B. White

Electronic Theses and Dissertations

BACKGROUND: The Affordable Care Act, enacted in 2010, transformed healthcare policy and forced hospitals to reevaluate traditional methods for care delivery. Researcher advocated for patient-centered models of care to reduce costs, address inequitable access to services, and improve service quality. These models prioritize patient values, preferences, and beliefs inclusive of patients’ religious and spiritual needs. Professional chaplains provide religious and spiritual care within many hospital settings. This dissertation explored the characteristics of hospitalized persons using chaplains, the factors associated with a hospital reporting a chaplaincy department, and how those services impacted patient satisfaction. METHODS: The first analysis used a two-part …


Literacy Abilities Of Children And Adolescents With Williams Syndrome., Caroline Greiner De Magalhaes Dec 2021

Literacy Abilities Of Children And Adolescents With Williams Syndrome., Caroline Greiner De Magalhaes

Electronic Theses and Dissertations

In this dissertation I report findings from two studies of the literacy abilities of children with Williams syndrome (WS), a genetic disorder associated with intellectual disability. I had two overarching goals: 1) evaluate the applicability of theories of reading and spelling acquisition developed based on typically developing children to children with WS; and 2) provide results that would inform the development of targeted assessments and interventions. In Study 1, individual differences in phonological awareness, visual spatial perception, vocabulary, overall intellectual abilities, and reading instruction approach (systematic phonics vs. other approaches) for sixty-nine 6 – 7-year-olds (most of whom were in …


Local Feature Selection For Multiple Instance Learning With Applications., Aliasghar Shahrjooihaghighi Dec 2021

Local Feature Selection For Multiple Instance Learning With Applications., Aliasghar Shahrjooihaghighi

Electronic Theses and Dissertations

Feature selection is a data processing approach that has been successfully and effectively used in developing machine learning algorithms for various applications. It has been proven to effectively reduce the dimensionality of the data and increase the accuracy and interpretability of machine learning algorithms. Conventional feature selection algorithms assume that there is an optimal global subset of features for the whole sample space. Thus, only one global subset of relevant features is learned. An alternative approach is based on the concept of Local Feature Selection (LFS), where each training sample can have its own subset of relevant features. Multiple Instance …


Charting The Unknown: Examining The Prevalence And Correlates Of Seconday Traumatic Stress In Kentucky Probation And Parole Officers., Amanda Marie Roberts Dec 2021

Charting The Unknown: Examining The Prevalence And Correlates Of Seconday Traumatic Stress In Kentucky Probation And Parole Officers., Amanda Marie Roberts

Electronic Theses and Dissertations

The purpose of this dissertation was to discern the prevalence and magnitude of both exposure to traumatic events and Secondary Traumatic Stress (STS); as well as potential correlates of STS in a group of Probation and Parole officers in the state of Kentucky Department of Corrections (KY DOC). Participants (N=302) completed a written survey which asked them to indicate demographic variables (age, gender, and ethnicity), as well as several important occupational variables, namely years of experience in the KY DOC, caseload volume, prison work experience, and whether or not they are responsible for a sexual offender caseload. Participants were also …


Drone Location And Scheduling Problems In Humanitarian Logistics., Zabih Ghelichi Dec 2021

Drone Location And Scheduling Problems In Humanitarian Logistics., Zabih Ghelichi

Electronic Theses and Dissertations

Unmanned Aerial Vehicles (UAVs), commonly referred to as drones, are a promising technology for the last-mile delivery of medical and aid items in humanitarian logistics. In emergency scenarios, like disasters, where transportation networks are destroyed and people are stranded, drones can accelerate the delivery of urgently needed items, e.g., food and water, insulin shots and blood pressure pills, to those trapped in the disaster-affected areas. Drones can also provide logistics services in many non-emergency situations by delivering medical items, e.g., vaccine shots and lab specimens, to remote communities and hard-to-access locations. The contribution of using UAVs goes beyond merely having …


Social Disorganization And Violent Crime Across Nonmetropolitan Areas Of Kentucky., Daniel Ray Jude Dec 2021

Social Disorganization And Violent Crime Across Nonmetropolitan Areas Of Kentucky., Daniel Ray Jude

Electronic Theses and Dissertations

This macro-level study examines the effects of social disorganization predictors on violent crime registered in nonmetropolitan areas of Kentucky from 2012 to 2016. The study intends to expand the line of research interested in verifying the applicability of the social disorganization theory (Shaw & McKay, 1942), including its new theoretical developments, to non-urban settings and plans to contribute to the literature willing to provide a better understanding of violent crime in rural areas. In response to Narag et al.’s (2009) call for a theoretical integration of environmental contaminants among the ecological factors that influence variations in crime, the study also …


A Remote Sensing Perspective: Mapping The Human Footprint In The Zambezi Region Of Namibia., Ariel E Weaver Dec 2021

A Remote Sensing Perspective: Mapping The Human Footprint In The Zambezi Region Of Namibia., Ariel E Weaver

Electronic Theses and Dissertations

The “human footprint” can be used as a general proxy to estimate human activities across the landscape. The human footprint in the Zambezi Region of Namibia is critically important for regional management of conservation efforts and land use planning. The land covers in the Zambezi Region are characteristically difficult to separate spectrally, due to a highly heterogeneous savanna landscape. Object Based Image Analysis (OBIA) and Random Forest (RF) methods are notable for their ability to improve classification accuracies of remotely sensed imagery. In this study, I investigate the extent of the human footprint in the Zambezi Region of Namibia, using …


Manifestations Of Students’ Voices: Examining Shifts, Academic Demands, And Identity Work In How Students Make Themselves Understood., Lauren Elizabeth Fletcher Dec 2021

Manifestations Of Students’ Voices: Examining Shifts, Academic Demands, And Identity Work In How Students Make Themselves Understood., Lauren Elizabeth Fletcher

Electronic Theses and Dissertations

Voice is a concept that is both highly sought after and elusive in education. While schools aim to foster students’ voices, many academic structures inadvertently conceal their voices and in turn their identities. Definitions of voice have been assumed, vague, or looked at as a writing trait, with little consideration of voices’ dynamic and mediated structures. Drawing on scholarship grounded in sociocultural theories and dialogism (e.g., Bakhtin, 1986; Engeström; 1987, Leont’ev, 1981; Rosenblatt, 1978; Vygotsky, 1978), I contribute a new, tangible definition of voice, in which voice is a dynamic happening, continually negotiated and constructed. This dissertation explores students’ voices, …


Exploring The Promotion Of Youth Voice And Activism By Youth Development Workers In Community-Based Programs., Rebecka M. Bloomer Dec 2021

Exploring The Promotion Of Youth Voice And Activism By Youth Development Workers In Community-Based Programs., Rebecka M. Bloomer

Electronic Theses and Dissertations

Youth-adult partnerships (Y-APs) and youth voice promotion are best practices within the youth development sector, but youth development workers receive little training or guidance in employing these concepts. A scarcity of research explores the relationships between organizational supports, as demonstrated by training, supervision, evaluation, and worker engagement in promoting youth voice. The two studies within this dissertation investigated the social processes of how and why youth development workers promoted youth voice. Program participants were primarily youth of color living in areas of high multidimensional poverty. They also explored how and why organizational factors impacted the promotion of youth voice within …


An Exploration Of Afrocentric Features At A Black Homeschool Collective., Tytianna Nikia Maria Wells Dec 2021

An Exploration Of Afrocentric Features At A Black Homeschool Collective., Tytianna Nikia Maria Wells

Electronic Theses and Dissertations

This dissertation explores the development and benefits of familial relationships as the primary feature of Afrocentricity at Black Scholars Academy (BSA), a Pre-Kindergarten (Pre-K) through 12th-grade Black homeschool collective in the United States. This qualitative study draws upon Afrocentricity as a theoretical framework to analyze and interpret data collected from classroom observations, individual interviews with current and former teachers and students, and textual artifacts between July and November 2019. Familial relationships—an African time orientation, a personalized learning plan, OurStory, and Rising Meeting—are present in BSA. Familial relationships helped students develop cultural pride, agency, self-determination, independence, liberation through education, and a …


Effects Of Chemosensory Experience And Context On Consummatory Behaviors., Saphira M. Chiu Dec 2021

Effects Of Chemosensory Experience And Context On Consummatory Behaviors., Saphira M. Chiu

Electronic Theses and Dissertations

Eating food generates associations between odors and tastes (i.e., flavor) that guide future choices. Experience with an odor-taste mixture links an odor with a taste’s quality and hedonic value, resulting in a preference for an odor paired with a palatable taste over an odor paired with an unpalatable taste. However, experience with a neutral stimulus (i.e., latent inhibition) or environment (i.e., context) can influence the formation of conditioned associations. Here, I used a two-bottle brief-access task to determine whether rats display an innate preference between unpaired odors (isoamyl acetate and benzaldehyde), how preexposure to the unpaired odors impacts mixture-dependent consummatory …


The Forgotten Ones: A Qualitative Study To Understand The Phenomena Of Medically Disqualified Student-Athletes., Regina Chanel Johnson Dec 2021

The Forgotten Ones: A Qualitative Study To Understand The Phenomena Of Medically Disqualified Student-Athletes., Regina Chanel Johnson

Electronic Theses and Dissertations

The purpose of this phenomenological study was to gain a better understanding of the experiences of medically disqualified student-athletes (MDSA) at National Collegiate Athletic Association (NCAA) Division I institutions. This study is significant because few studies have examined MDSAs, yet they present concerns around career-ending injuries. Drawing on the Kübler-Ross Model of Death and Dying (1969) framework, this study examined if MDSAs have faced experiences in stage-like forms such as denial, anger, bargaining, depression, and acceptance. This study called the attention of athletic administrators to examine and develop support resources for this often-overlooked student-athlete group. Over five chapters, this dissertation …


Collaborative Human-Machine Interfaces For Mobile Manipulators., Shamsudeen Olawale Abubakar Dec 2021

Collaborative Human-Machine Interfaces For Mobile Manipulators., Shamsudeen Olawale Abubakar

Electronic Theses and Dissertations

The use of mobile manipulators in service industries as both agents in physical Human Robot Interaction (pHRI) and for social interactions has been on the increase in recent times due to necessities like compensating for workforce shortages and enabling safer and more efficient operations amongst other reasons. Collaborative robots, or co-bots, are robots that are developed for use with human interaction through direct contact or close proximity in a shared space with the human users. The work presented in this dissertation focuses on the design, implementation and analysis of components for the next-generation collaborative human machine interfaces (CHMI) needed for …


Soil Gypsum Content Analysis Of The Proposed Road Route Extension Of Fm 2185 In The Gypsum Plain, Culberson County, Texas, Chikaodili Ohakawa Dec 2021

Soil Gypsum Content Analysis Of The Proposed Road Route Extension Of Fm 2185 In The Gypsum Plain, Culberson County, Texas, Chikaodili Ohakawa

Electronic Theses and Dissertations

Soils serve as the underlying substrate on which foundations for most structures are built. The properties of a soil type are therefore an important consideration in building engineered structures. Gypsic soils are problematic for construction of such structures. One of the main problems with gypsic soils is dissolution. Gypsum (CaSO4·2H2O) in soils dissolves easily creating voids which can be detrimental to the integrity of engineering structures. To mitigate this, it is important to determine the amount of gypsum present in soils, before construction is initiated.

In this study, three methods were employed in the determination of …


The Role Of Embodied Metaphor In The Relationship Between Vestibular Dysfunction, Anxiety And The Self, Kaitlyn Abrams Dec 2021

The Role Of Embodied Metaphor In The Relationship Between Vestibular Dysfunction, Anxiety And The Self, Kaitlyn Abrams

Electronic Theses and Dissertations

There is considerable evidence that vestibular dysfunction and anxiety are correlated. Clinical and philosophical arguments suggest that a loss of sense of self, experienced through symptoms like derealization and depersonalization, might contribute to this relationship. The present study used a mixed methods approach, including Systematic Metaphor Analysis, to analyze written short answer and quantitative survey data from one hundred and thirteen participants with vestibular disorders. The Systematic Metaphor Analysis revealed several primary metaphor concepts which elucidated the connections between concrete experience and abstract concepts in vestibular dysfunction. These included metaphors of Agency, Stability, Orientation, Self-Object, and Figure-Ground. Primary metaphors for …


Preclinical Development Of Epicertin, A Novel Biotherapeutic For The Treatment Of Ulcerative Colitis., Micaela Ann Reeves Dec 2021

Preclinical Development Of Epicertin, A Novel Biotherapeutic For The Treatment Of Ulcerative Colitis., Micaela Ann Reeves

Electronic Theses and Dissertations

Epicertin (EPT) is a recombinant variant of the cholera toxin B subunit with a C-terminal KDEL endoplasmic retention motif. Previously, orally administered EPT demonstrated mucosal healing activity in acute and chronic dextran sulfate sodium colitis models, indicating its therapeutic potential for ulcerative colitis (UC) treatment. However, oral dosing with EPT requires neutralization of gastric acid prior to administration, hindering its facile application in the treatment of chronic diseases like UC. One solution to this issue is that EPT may also be administered intracolonically without losing efficacy. This work describes the determination of a target intracolonic dose of EPT for future …


Pyridine Nucleotide Redox Potential In Coronary Smooth Muscle Couples Myocardial Blood Flow To Cardiac Metabolism., Marc Matthew Dwenger Dec 2021

Pyridine Nucleotide Redox Potential In Coronary Smooth Muscle Couples Myocardial Blood Flow To Cardiac Metabolism., Marc Matthew Dwenger

Electronic Theses and Dissertations

The maintenance of myocardial oxygen supply during stress is essential for sustaining cardiac health. Enhancement of coronary blood flow upon increases in myocardial oxygen demand (i.e., hyperemia) relies on regulation of voltage-gated K+ (Kv) channels by their intracellular β subunits (i.e., Kvβ proteins). Considering that, Kvβ proteins are aldo-keto reductases (AKRs) and regulate Kv channel gating, we tested the hypothesis that elevation of myocardial oxygen demand modifies intracellular NAD(H) in arterial myocytes. Furthermore, we tested whether the resultant change in the redox state of the pyridine nucleotide pool directly regulates coronary Kv1 channel activity. High-resolution imaging mass spectrometry and …


Investigations Of Potential Mechanisms Underlying Spinal Cord Injury-Induced Polyuria., Jason H. Gumbel Dec 2021

Investigations Of Potential Mechanisms Underlying Spinal Cord Injury-Induced Polyuria., Jason H. Gumbel

Electronic Theses and Dissertations

Spinal cord injury (SCI) results in neurological impairments including motor, sensory, and autonomic dysfunction. These neurological deficits result in a litany of complications apart from muscular paralysis, including bladder, bowel, cardiovascular, and sexual function. SCI-induced polyuria (the overproduction/passage of urine) remains understudied, and therefore mechanisms behind it are largely unknown and require extensive investigation for potential targeted therapies to improve quality of life. The objective of this dissertation was to investigate potential mechanisms of SCI-induced polyuria and explore potential therapies to improve quality of life in the SCI population. Metabolic cages, Western blot, enzyme-linked immunoassay, and immunostaining were first used …


Exploring Service Provider Perspectives On Facilitators And Barriers To Needle Exchange Program Participation By Females Who Inject Drugs., Tammi Alvey Thomas Dec 2021

Exploring Service Provider Perspectives On Facilitators And Barriers To Needle Exchange Program Participation By Females Who Inject Drugs., Tammi Alvey Thomas

Electronic Theses and Dissertations

As the opioid epidemic lingers on across the country, many areas have set up harm reduction strategies such as needle exchange programs (NEPs) to combat the long-term consequences of injection drug use (IDU). Males and females face a plethora of health issues associated with injection drug use such as human immunodeficiency virus (HIV) and hepatitis C. While males comprise the largest portion of the injection drug use population, most research is gender neutral, which makes it difficult to discern issues specifically related to females inhibiting our ability to design interventions and procedures targeted to address their needs. Females require varying …


Impact Of Pank1 Deletion On Mitochondrial Acetylation And Cardiac Function During Pressure Overload., Timothy N. Audam Dec 2021

Impact Of Pank1 Deletion On Mitochondrial Acetylation And Cardiac Function During Pressure Overload., Timothy N. Audam

Electronic Theses and Dissertations

Recent studies have associated elevated protein acetylation levels with heart failure in humans. Although mechanisms promoting elevated acetylation levels are not fully known, excess acetyl-CoA may drive enzyme-independent acetylation of cardiac proteins. Accumulation of acetyl-CoA depends on the availability of sufficient CoA, whose production is regulated by pantothenate kinases in the CoA biosynthetic pathway. We show that cardiac proteins are hyperacetylated during heart failure in humans and tested in mice whether limiting CoA abundance would improve ventricular remodeling during pressure overload-induced hypertrophy. We limited cardiac CoA levels by deleting the rate-limiting enzyme in CoA biosynthesis, Pank1 (one of three PANK-encoding …