Digital Commons Network^™

Crispr/Cas9 Whole-Genome Screens Identify Genes Critical For Neutral Lipid Metabolism In Macrophages, Rifat Sultana

Electronic Theses and Dissertations

Lipid-laden macrophages contribute to atherosclerotic plaque formation in atherosclerosisrelated heart diseases. Thus, understanding formation and degradation of lipid would help identify therapeutic targets for the treatment of atherosclerosis. In the first chapter of this dissertation, I have explained the current understanding of cholesterol metabolism in macrophages and how mTOR could play a potential role in this process. Additionally, I have described how CRISPR/Cas9 whole-genome screens present an effective tool to identify genes regulating cholesterol metabolism including positive and negative regulators of lipid droplet formation and lipid droplet degradation. In Chapter 2, I have described the results of the CRISPR/Cas9 whole-genome …

Genome-Wide Association Mapping And Genomic Prediction For Enhancing Fhb Resistance In Hard Winter Wheat, Jinfeng Zhang

Electronic Theses and Dissertations

Wheat is one of the most important staple crops providing 20% of energy for 35% of the world population. Fusarium head blight (FHB), primarily caused by the fugal Fusarium graminearum Schwabe, is a damaging disease in wheat that affects global wheat production every year and causes food safety issues. The disease not only reduces the grain yield and quality but also produces mycotoxin in the diseased kernels making them unsuitable for human consumption or as livestock feeds. Breeding FHB resistant cultivar is the most effective and economical approach to managing the disease. This study combines genome-wide association study (GWAS) and …

Efficacy Of Nanomaterials And Biorationals On The Bacterial Leaf Streak Management In Wheat, Abraham Hangamaisho

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is one of the most important cereal crops in the United States. Most wheat varieties are susceptible to bacterial leaf streak (BLS), a major disease caused by Xanthomonas translucens pv. undulosa (Xtpvu). BLS is challenging to manage since common chemicals do not provide adequate control. Nanomaterials and plant extracts have shown potential to provide a sustainable environmentally friendly control of animal and plant diseases. However, limited data are available on the efficacy of plant extracts and nanomaterials controlling BLS in wheat. The objective of this study was to evaluate the antibacterial activity of plant extracts and …

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …

Design, Synthesis, Structure Optimization, Structure-Activity Relationship (Sar), And Biological Evaluation Of Novel And Potent Thiazolo[4,5-D]Pyrimidine Corticotropin Releasing Factor (Crf) Receptor Antagonists, Md Rabiul Islam

Electronic Theses and Dissertations

No abstract provided.

Disentangling The Complex Evolutionary History Of Invasive Russian Thistle: Phylogenetic Relationships, The Role Of Hybridization, And Public Engagement, Deva Raj Khanal

Electronic Theses and Dissertations

Invasive species are forces of environmental, economic, and social change with detrimental impacts on ecosystems, commerce, agriculture, and human health. However, we still have a poor understanding of the processes that could be driving invasive potential. Hybridization may contribute to the establishment and spread of invasive lineages, as it may enhance genetic variation and facilitate rapid adaptation. Focusing on Russian thistle (Salsola tragus s.l., family Amaranthaceae) and closely related species, my thesis aims to clarify phylogenetic relationships and investigate how hybridization has shaped the invasion dynamics of these lineages. Known as one of the fastest plant invasions in the United …

Prairie Cordgrass Cellulose: A Sustainable Resource To Develop Biodegradable Films, Mominul Hoque

Electronic Theses and Dissertations

Plastics are versatile and cost-effective materials with a wide variety of functionalities. Their non-biodegradability, however, is an environmental concern. Plastic waste accumulation in landfills and natural habitats results in health issues, mainly due to the leaching of chemicals from plastic products that further transfer to humans and wildlife. Many countries around the globe have imposed restrictions on plastic use. Despite these concerted efforts, a pressing scientific need exists to find an alternative to plastics. Cellulose from abundant agricultural biomass stands out as a viable alternative due to its robust structure, biocompatibility, biodegradability, low density, and non-toxicity. This research aims to …

A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud

Electronic Theses and Dissertations

Pneumonia is a major factor affecting populations of free-ranging bighorn sheep (Ovis canadensis) across western North America. Pneumonia can occur in large-scale epizootics, during which greater than half of the population typically dies. After these epizootics, surviving ewes continue to conceive and bear lambs. However, lamb recruitment may remain low due to periodic or annual pneumonia outbreaks causing high lamb mortality rates, sometimes greater than 90%. Our study focused on the Rapid City, South Dakota bighorn sheep (BHS) herd that has recorded pneumonia-induced population decline since 2009. The first objective was to improve lamb health and survival by identifying and …

Northern Great Plains Saline Sodic Soil Development, Classification, Remediation, And Management., Shaina Westhoff

Electronic Theses and Dissertations

Soil salinity and sodicity are issues of growing concern in the United States (U.S.) and globally. Knowledge gaps for glaciated, dryland salt-affected soils exist because much original salinity and sodicity research focused on irrigated systems. Land managers are being asked to produce food, feed, fiber, and fuel for an expanding global population. The number of land managers and crop advisors who are affected by these soils is increasing. Addressing salinity and sodicity knowledge gaps will be critical for their success. Salinity and sodicity have been impeding crop productivity since the advent of cultivation. Saline and sodic soils form via multiple …

“Damned If Ya Do, Damned If Ya Don’T”: A Critical Narrative Inquiry Exploring The Gendered Racism Experienced By Black Women Housing Professionals In Higher Education, Shaniquè Jazmine Broom

Electronic Theses and Dissertations

Between 1999 and 2018, there was an 11% decrease in Black women staff and administrators at post-secondary institutions. This study utilized Black Feminist Thought and Sista Circle Methodology to uncover how Black women reflected on experiences of and coped with gendered racism at PWIs. Participants offered reflections on their relationships with Black women and men, white men and women, and students. Black women shared their reflections with discrimination and a deceptive institutional culture. Black women also discussed utilizing several coping strategies such as hyper-awareness, hypervigilance, enacting personal and professional boundaries, avoiding hypervisibility and engaging in personal and familial connections with …

Development And Initial Validation Of An Acculturation Measure For Arab Expatriates In Qatar Using Mixed Methods, Sara Ali Ahmed Zikri

Electronic Theses and Dissertations

Prior research on acculturation has predominantly focused on specific immigrant and ethnic groups in the United States, Europe, and Asia, and there is a lack of publicly available scales that measure acculturation among expatriates in countries of the Gulf Cooperation Council. The purpose of this study was to use a mixed methods research design to develop a theoretical framework of acculturation for white-collar Arab expatriates in Qatar as well as a culturally appropriate self-report acculturation measure. The acculturation stories and experiences of white-collar Arab expatriates in Qatar were qualitatively explored and analyzed, and their findings were used to construct an …

Transition Barriers Of U.S. Military Veterans With Combat Occupations, Carole Thomas

Electronic Theses and Dissertations

Many combat-occupation veterans experience difficulty translating their military occupation to a civilian occupation after they leave the military. Veterans may struggle with military culture, self-leadership, health concerns, stereotypes, and employment barriers such as disability and underemployment. In this qualitative descriptive research study, the researcher analyzed the transition barriers U.S. combat-occupation veterans experienced when entering the civilian sector and workforce. The researcher interviewed six participants via Zoom using semistructured interview questions. All participants met the criteria of being 18–29 years old, being a veteran with a combat occupation, and having a willingness to tell their transition experiences. Five of the six …

Making The Most Of Program Evaluation Data: Understanding Human Services Professionals’ Well-Being Through Qualitative Secondary Analysis, Elizabeth Ann Deaton Wacker

Electronic Theses and Dissertations

Qualitative secondary analysis (QSA) allows researchers to explore new research questions and ensure that participants’ voices are heard to the greatest extent possible, without the burdens of additional data collection. However, this approach is rarely used outside of the health sciences, and little guidance exists in the literature about how to conduct QSA. This study is a secondary analysis of qualitative program evaluation data related to the well-being of human services professionals from two fields: child welfare and early care and education (ECE). It explores these professionals’ well-being, as well as the methodological issues of how well-being has been addressed …

A Call For Critically Inclined Higher Education Data, Wendy A. Fish

Electronic Theses and Dissertations

The economic logic of neoliberalism has reached its zenith—now firmly entrenched in our political, social, and cultural transactions (Giroux, 2014; Harvey, 2005). As a result, we stand on the precipice of a new era—the data era—in which neoliberalism’s orientation towards accountability, micro-management, and hierarchical ordering mediates all social and political narratives. Data, it is said, is fast becoming “the new oil”—the most valuable global resource (Bhageshpur, 2019; Harari, 2018).

This is problematic. Although there are countless ways in which the application of data has improved the world, it has a long, dark history as a technology of oppression. Yet, data …

Policymaker Discourse In Colorado Public Higher Education Appropriations, Nathan Willers

Electronic Theses and Dissertations

State funding for higher education is one of the most pressing issues facing both institutions and policymakers in 2022. Colorado has seen significant declines in the amount of state funding per student over the past two decades, resulting in increases in student burdens for tuition. Policy discourse analysis through a poststructural and neoliberal lens provided insight towards the legislative process and the discursive themes utilized by Colorado policymakers during the 2021-22 legislative budgeting cycle. Twelve artifacts were analyzed, including a mix of policy briefs, hearings, and budget and appropriations reports, for discursive data related to the state’s appropriations for public …

The Immobilization Of Ticagrelor On Implant Materials And Corrosion And Biofouling Inhibition By Novel Layered Polymer Thin Films For Marine Application, Tell Lovelace

Electronic Theses and Dissertations

Percutaneous coronary interventions are a widely used cardiovascular surgery which is typically accompanied by the insertion of a vascular stent. After the procedure patients are put on dual anti-platelet therapy (DAPT) to prevent stent thrombosis, a common complication to this procedure in which platelets aggregate in the blood vessel on the inserted stent. Systemic deactivation of platelets in patients via oral DAPT results in patients becoming bleeding risks in future medical procedures, necessitating the development of a localized delivery system for anti-platelet medications from the surface of the stent. A novel anti-thrombogenic vascular stent coating has been prepared by covalent …

Building A New Model Of Church-State Collaboration On Environmental Care In Ghana Based On Catholic Social Teaching And African Theological Traditions, Emmanuel Abbey-Quaye

Electronic Theses and Dissertations

Abstract

The care of the environment has always been considered by the Ghana Catholic Church as an important aspect of her pastoral care and evangelizing mission in Ghana. Hence, in their various teachings in communiqués and other pastoral documents from 1965 till now, the Ghana Catholic Bishops’ Conference has consistently addressed the major environmental challenges confronting Ghana such as deforestation, mining, pollution, etc., applying the principles of stewardship and care of creation, intergenerational solidarity, integral ecology, etc., to ground these teachings.

Besides, in acknowledging that neither the Catholic Church nor the Ghanaian Government alone can resolve the various ecological crises …

Culturally Therapeutic Approaches To Prejudice And Discrimination: Formulations, Interventions, And Structural Change, Jayme J. Jenkins

Electronic Theses and Dissertations

Aims. Though psychologists are generally well-positioned to implement micro- and macro-level interventions from their roles as clinicians, educators, researchers, and consultants, there is a paucity of formal guidance on how to approach client-generated or structural prejudice and discrimination. The current study addresses this literature gap by integrating previous literature and implementation models from a related field toward a novel treatment approach.

Background. A critical literature review supported intervention-focused conceptualizations of “supremacism” and demonstrated that mutually reinforcing micro- and macro-level discrimination generates demographic disparities in safety, liberty, and well-being. This typically emerges in the United States as White supremacist heteropatriarchy while …

Hip Muscle Function In Individuals With Chronic Ankle Instability, Lindsay Carroll

Electronic Theses and Dissertations

Purpose: Hip muscle function has been reported to be altered in individuals with chronic ankle instability (CAI). The purpose of this study was (1) to determine whether the single leg squat test (SLST) could be used to detect differences in proximal muscle activation between individuals with and without CAI and (2) to determine if there was a difference in maximum isometric body weight normalized torque output of the hip lateral rotators, extensors, and abductors between individuals with and without CAI.

Subjects: Forty-eight subjects (14 males, 34 females; median age = 22.00 years, interquartile range (IQR) = 1.00; median height = …

Relational And Social Aggression: A Scoping Literature Review, Angela Fidazzo

Electronic Theses and Dissertations

School violence is an omnipresent problem within the United States, at worst, resulting in the death of children and teachers across the nation. Over time, numerous strategies and tools have been introduced in order to reduce aggression within schools. However, as both overt and covert forms of aggression contribute to school violence, it is necessary for researchers to investigate covert, indirect forms of aggression as a way to better identify and prevent a salient contribution to this problem. Although much research has investigated bullying in general, further research is needed in better understanding the forms of covert bullying, relational and …

Counselors Dumbfounded: Implications For Counseling Ethics, Ivan Irizarry

Electronic Theses and Dissertations

How counselors should make ethical decisions frequently differs from how counselors actually make ethical decisions, which is often a non-linear process. Social Intuitionist Theory (Haidt, 2003) suggests this process entails a socially influenced intuitive judgment followed by post hoc rationale. The purpose of this study was to examine whether moral dumbfounding, which entails maintaining a moral judgment without supporting reason, occurs with practicing counselors. The results of survey data and open-ended coding showed that 58.5% of counselors were dumbfounded at least once when presented with four moral or ethical vignettes. Additional analyses exploring differences in dilemma-based vs. non-counseling vignettes, moral …

The Effect Of School-Based Mindfulness Intervention On Student Attention And Executive Function: A Meta-Analysis, Sarah E. Rea

Electronic Theses and Dissertations

Schools are facing increasing responsibility to foster the social-emotional development of students. One way in which schools can improve student functioning is through school-based mindfulness interventions. Using mindfulness practices, in particular, can teach students to increase their attention of surroundings and internal experiences, and awareness of their thoughts and behaviors. While the evidence-base for mindfulness interventions in schools continues to grow, there are several studies that show promising outcomes. The purpose of this study was to determine how large the effect sizes for school-based mindfulness studies are in regard to increasing student attention, cognitive flexibility, and working memory. Results indicate …

Detecting Viral Particles In Vitro Using Photoacoustic Flow Cytometry, Anie-Pier Samson

Electronic Theses and Dissertations

The Covid-19 pandemic is a powerful example of just how damaging the rapid spread of an unknown virus can be. Viruses have the ability to spread rapidly amongst individuals if not treated and controlled. The first key step towards treatment is the timely and specific diagnosis of the virus causing the infection. An innovative method for the rapid detection of viral particles in solution consists of tagging the viral particles paired with using photoacoustic flow cytometry to irradiate the particles and get a signal. The high affinity of Streptavidin for Biotin can be used to bind Streptavidin-coated microspheres to biotinylated …

Cgrp-Driven Lateralization Of Pain Modulation By The Central Amygdala, Heather Allen

Electronic Theses and Dissertations

The central amygdala (CeA) is a bilateral, mid limbic brain region involved in the processing of pain and emotion. Interestingly, the left and right CeA are functionally lateralized in the context of bladder pain, with the left and right CeA having opposing effects on bladder pain-like behavior. However, the specific mechanisms driving these divergent functions are unknown. Nociceptive signals from the bladder are relayed to the CeA via projection neurons in the parabrachial nucleus (PBN) that express high levels of calcitonin gene-related peptide (CGRP). In these studies, we investigated the influence of PBN CGRP signaling in the left and right …

Connecting Human Dignity, Non-Violence, And Environment For Healthcare Ethics In Nigeria, John Mark Ogu

Electronic Theses and Dissertations

Abstract

Connecting Human Dignity, Non-violence, and Environment in Health Care for Nigeria

By

John Mark Chinaemerem Ogu

December 2021

Dissertation supervised by Peter Ikechukwu Osuji, Ph.D.

This dissertation presents a global public health ethical approach based on social justice that connects human dignity, non-violence, and the environment in Nigeria. Recently, different places globally are witnessing uprisings and violence; some of these are linked to environmental scarcity. These are threats to global health, peace, development, and security because violence of any type or form is a global public health and security menace and an affront to human dignity. Environmental scarcity, especially …

Investigating Relationships Between Diabetes Distress, Diabetes Self-Care Behaviors, Intention To Use And Actual Use Of A Personal Health Record Within 30 Days Of Hospital Discharge In Persons With Type 2 Diabetes, Khaliah Fisher-Grace

Electronic Theses and Dissertations

Background: Diabetes distress (DD) is defined by emotional and behavioral challenges caused from diabetes and its management. Diabetes self-care behaviors can be compromised by DD. Personal Health Record (PHR) use has been associated with improvements in HbA1c, blood pressure, and LDL-C, but the relationship between DD and PHR use is unknown. Aims: 1) to determine differences between sociodemographic variables and intention to use and actual use of PHR in hospitalized persons with Type 2 Diabetes Mellitus (T2DM), 2) to investigate the relationship between DD, intention to use a PHR and actual use of a PHR within 30 days of hospital …

Forced Together, Growing Apart: An Exploration Of Heterosexual African American Males' Disengagement In Romantic Relationships With African American Females, Kenya Johns

Electronic Theses and Dissertations

The decline of intraracial dating has been more pronounced among African Americans in comparison to other racial groups. There is lack of available research tailored towards Black men’s dating experiences. The purpose of this study was to explore the lived experiences of heterosexual African American males related to their disengagement in romantic relationships with African American females.

A phenomenological study explored the lived experiences of 10 African American males and the dynamics that appear to contribute to the decline in intraracial dating in the African American community. Five themes emerged from the study: – self-regard, family matters, proximity, the new …

Design And Synthesis Of Fused Pyrimidines As Single Agents With Combination Chemotherapy Potential, As Tubulin Polymerization And One-Carbon Metabolism Inhibitors For Cancer, Tasdique Quadery

Electronic Theses and Dissertations

This dissertation describes the design, synthesis, and biological evaluation of bicyclic fused pyrimidines as single agents with combination chemotherapy potential with both antiangiogenic and cytotoxic effects. The dissertation also discusses one-carbon metabolism inhibitors and tumor targeting proteolysis targeting chimeras (PROTACs).

In cancer chemotherapy, the two major limitations are the dose-limiting toxicities of clinically used agents and the development of resistance to the treatment. Combination chemotherapy with antiangiogenic agents and microtubule targeting agents has shown an advantage against both these drawbacks. Single agents with both antiangiogenic activity and cytotoxicity would afford a therapy that circumvents pharmacokinetic problems of multiple agents, avoid …

Real-Time Monitoring Of Cell Death Progress Using Capacitance Spectroscopy, Suyang Wu

Electronic Theses and Dissertations

Biologics, including the monoclonal antibody (mAb), has experienced rapid development in the last decade. However, the price of biologics is often prohibitively high because of the low process efficiency. Delaying the inevitable cell death improves the productivity of upstream bioprocessing, whose success relies on monitoring the cell death onset that indicates the timing for preventive actions.

This study proposes to develop a real-time monitoring model that quantifies the dying cell percentage in lab-scale bioreactors using capacitance spectroscopy. The capacitance spectroscopy contains cell death-related information due to various physical properties changes during the cell death process, e.g., cytoplasmic conductivity change. The …

The Contribution Of Bioethics To Home Healthcare (Hhc) In Saudi Arabia, Saeed Alghamdi

Electronic Theses and Dissertations

This dissertation examines the contribution of bioethics to Home Healthcare (HHC) In Saudi Arabia (SA). Through exploring the critical bioethical issues that have influenced the implementation of home health care, the dissertation highlights the significance of incorporating bioethics in home health care. Organizational ethics has led to the regulation of healthcare professionals, patients, and the stakeholders within clinical care settings. Healthcare organizations and other stakeholders, such as global health bodies, should formulate laws and policies that promote bioethics in contemporary healthcare. The Kingdom of Saudi Arabia, through the Ministry of Health, should promote the adoption of home health care services …