Digital Commons Network^™

Building An Affirmative Lgbtq+ Leadership Paradigm: An Autoethnography., Joshua D. Covington

Electronic Theses and Dissertations

This study is an autoethnography written in the form of layered accounts with a theoretical framework of queer theory. It was based on my journey as an LGBTQ+ individual and educational leader. The purpose of this study was to utilize my experiences as an LGBTQ+ educational leader to investigate the conditions that supported and limited my self-efficacy as an LGBTQ+ educational leader. Furthermore, this study investigated what things my experiences could teach us about building an affirmative LGBTQ+ leadership paradigm. My autoethnography was written as three significant stages of my life. The first stage was my experience with education as …

The Role Of E-Liquid Constituents In E-Cigarette-Induced Cardiac Arrhythmia And Autonomic Imbalance., Cory Kucera

Electronic Theses and Dissertations

Introduction. Accumulating evidence indicates that exposure to electronic cigarettes (e-cigs) promotes sympathetic dominance and electrophysiologic instability in the heart, potentially culminating in arrhythmogenesis. E-liquids contain various formulations of nicotine and flavorings, but the effects of specific e-liquid constituents and their concentrations on e-cig-induced autonomic imbalance and electrical dysfunction are unresolved. To that end, we tested the hypothesis that e-cigs modify cardiac autonomic balance and ventricular arrhythmogenesis in mice dependent on e-liquid constituent type and concentration. Methods. Using a crossover design and a serial exposure regimen, ECG-telemetered male C57BL/6J mice underwent whole-body exposure to e-cig aerosols generated from e-liquids containing different …

Changes To Water Quality And Discharge In An Urban Stream In Southwest Jefferson County., Karina Henson

Electronic Theses and Dissertations

Mill Creek is the largest watershed in Southwest Jefferson County, where thousands of people live and work. Streams in the watershed have historically been channelized and altered to promote drainage, and stream health is of major concern. Louisville MSD is currently developing a watershed plan for Mill Creek by seeking understanding of current stream conditions. This paper will focus on historical trends in stream discharge and water quality. Peak and daily discharge, dissolved oxygen, specific conductance, and temperature data from USGS gages at Mill Creek and the Mill Creek Cutoff was analyzed to understand how water quality and flow rates …

From Androgyny To Binary And Back: A Qualitative Analysis Of Nonbinary Tiktoks., Savannah Hatton

Electronic Theses and Dissertations

This study explores how nonbinary individuals represent and express their gender on TikTok. Given the limited amount of research that has been done on nonbinary gender expression in online spaces, this study uses TikTok to observe the ways that nonbinary individuals perform gender. Additionally, given the relative newness of TikTok, this study serves to inform growing research on identity formation through social media. It used a sample of fifty TikToks by nonbinary creators to perform a content analysis. The findings reveal significant variation in how nonbinary individuals "do gender" on the platform, with some individuals identifying closely with binary labels, …

Cannabidiol Tweet Miner: A Framework For Identifying Misinformation In Cbd Tweets., Jason Turner

Electronic Theses and Dissertations

As regulations surrounding cannabis continue to develop, the demand for cannabis-based products is on the rise. Despite not producing the psychoactive effects commonly associated with THC, products containing cannabidiol (CBD) have gained immense popularity in recent years as a potential treatment option for a range of conditions, particularly those associated with pain or sleep disorders. However, due to current federal policies, these products have yet to undergo comprehensive safety and efficacy testing. Fortunately, utilizing advanced natural language processing (NLP) techniques, data harvested from social networks have been employed to investigate various social trends within healthcare, such as disease tracking and …

Inhibition Of Ras As A Novel Therapeutic Approach In Luminal B Breast Cancer., Raphael Ngozichi Jigo

Electronic Theses and Dissertations

Historically, the RAS oncoprotein has not been implicated in breast cancer due to less than 1% of breast cancer cases bearing oncogenic RAS mutations. Recently, however, it has been reported that greater than 60% of Luminal B breast cancer cases have a decreased expression of negative RAS regulators RASAL2 and DAB2IP. Thus, in many Luminal B breast cancers, RAS remains in a constitutively active state without mutation, in turn driving oncogenesis. To date, there are no FDA-approved direct inhibitors of wild type RAS. We have developed a direct RAS inhibitor that acts on wild type RAS using in silico drug …

Investigation Of Possible Inflammatory Response After Ultrasound And Microbubble Procedure In The Spinal Cord., Jenna Wilkerson

Electronic Theses and Dissertations

The purpose of this experiment is to explore the inflammatory effects of focused ultrasound paired with intravenously delivered microbubbles when used in the lumbar spinal cord. There are currently very few pharmaceuticals that can successfully enter the blood spinal cord barrier (BSCB). This technique used at the lumbar enlargement might allow targeted treatment of pathologies involving the spinal cord.

To test the possible inflammatory response of this technique, an animal experiment was performed. 15 animals were given a T12 laminectomy and allowed to fully recover. Once recovered, all animals received FUS+MB at L2 and were euthanized in groups of five …

Addition Of Process Control Experiment To Unit Operations Ii Laboratory., Bradley Esselman

Electronic Theses and Dissertations

Process control is an essential aspect of manufacturing, contributing to improved process safety, efficiency, product consistency, and energy optimization. Chemical engineers are often responsible for ensuring the safe, efficient, and cost-effective operation of industrial processes, and process control plays a pivotal role in accomplishing this.

Chemical engineering students at the University of Louisville complete the course, Elements of Process Control, during the second semester of their fourth year. This lecture-based course offers limited hands-on, practical experience. To supplement the lack of hands-on experience in the classroom, a new process control experiment was designed and implemented into the Unit Operations …

Design And Evaluation Of A Multi-Functional Surgical Device For Microgravity Surgeries., Sienna Shacklette

Electronic Theses and Dissertations

No abstract provided.

The Initial Validation Of A Self-Care Belief And Behavior Questionnaire In The Iecmh Workforce, Julia Najm

Electronic Theses and Dissertations

The Infant and Early Child Mental Health (IECMH) workforce is essential in improving the lives of vulnerable children and families. IECMH practitioners are exposed to challenging emotional experiences which increases their risk of internalizing disorders, burnout, and emotion regulation difficulties, especially in the time of COVID-19. Thus, it is important to identify possible interventions to increase provider wellness in the face of taxing work. The construct of self-care offers a promising area of study as a possible intervention point. However, current operationalizations of self-care have been limited and have emphasized behavioral components rather than cognitive components (e.g., beliefs) pertinent to …

Secondary Educator And Administrator Perceptions Of Positive Behavior Interventions And Supports And Student Academic Achievement, Cynthia Everitt

Electronic Theses and Dissertations

This qualitative study addresses secondary educator perceptions of Positive Behavioral Interventions and Supports (PBIS) and student academic achievement. PBIS is a proactive approach with a multitiered framework. When used properly, PBIS can be a tool for school faculty to establish behavioral expectations and procedures, prevent disruptive behavior, and improve the school climate and culture. This phenomenological study was conducted using two secondary schools in West Virginia. Participants were identified and chosen through purposive sampling techniques according to their years of experience teaching and utilizing PBIS interventions.

The data for this study included unstructured, open-ended interviews based on three research questions. …

“Just As Scared As I Am”: Daughters’ Perspectives Of The Impact Of The Overturning Of Roe V. Wade On Mother-Daughter Reproductive Conversations, Casadie Smith

Electronic Theses and Dissertations

Existing literature has identified mothers as the primary reproductive educators of their daughters and has determined mother-daughter conversations about reproduction to be an indicator of daughters’ sexual and reproductive behaviors. The overturning of Roe v. Wade on June 24, 2022, had an immediate impact on the reproductive lives of many people, including daughters. Through one-on-one interviews with 10 daughters, this qualitative study identifies three changes in mother-daughter reproductive conversations that were influenced by the overturning of Roe v. Wade: (a) The initiation of conversations, (b) the topics of conversations, and (c) the tone of these conversations. The results of this …

Gratitude And Sleep Quality In Primary Care: Mediating Effects Of Health Self-Efficacy, Health Behaviors, And Psychopathology, Heather R. Altier

Electronic Theses and Dissertations

Sleep disturbances are prevalent among primary care patients, and psychological dysfunction, including stress, anxiety, and depression, are robust contributors to poor sleep health. Yet, the presence of potential protective characteristics, such as health self-efficacy and engaging in adaptive health behaviors, may mitigate such outcomes. Gratitude (i.e., recognition and appreciation of experiences, relationships, and surroundings), a positive psychological cognitive-emotional characteristic, may serve as a catalyst of these beneficial downstream effects, given its association with improved health functioning and sleep. In a sample of primary care patients (N = 869) from 50 urban and 30 rural practices in Germany, health self-efficacy …

Use Of Transposon Screening For Salicylic Acid-Assisted Desiccation Killing In Salmonella, Shannon D. Elliott

Electronic Theses and Dissertations

Salmonella enterica serovar Typhimurium is one of the most prevalent food-borne pathogens, affecting millions around the world every year, making it a threat to global health. Salmonella possesses the ability to survive the normally lethal condition of desiccation, however, discovery of the genes and mechanisms behind this phenomenon are still ongoing. Using a transposon mutagenesis approach to construct a broad transposon library, this study aimed to uncover genes that may be contributing to changes in Salmonella’s survivability under desiccation, particularly when exposed to the antimicrobial molecule salicylic acid. Building on previous findings showing salicylic acid can alter cell viability …

The Old Family Clock: Exploring High Variation Of Chronotype In The Common House Spider, Parasteatoda Tepidariorum, Caitlin Jones

Electronic Theses and Dissertations

Circadian rhythms are often tightly confined around a mean of 24 hours with little variation in traditional model organisms in the absence of environmental time cues. Some spider species fall well outside this normal range with mean periods of 18 hours and variation around these means of 6 hours or more. This extreme variation in circadian rhythm is not well-understood and is the focus of the current study. Using Parasteatoda tepidariorum as a model organism, we investigated whether variation in eight circadian parameters could be explained by genetic variability, environmental conditions, or an endogenous feature of the circadian system of …

Analyzing The Influence Of Mathematics Intervention Teacher Actions On Students' Mathematics Identities: An Examination Of Bipoc Student Perceptions And Past Learning Experiences., Sydni P. Morris

Electronic Theses and Dissertations

This dissertation examines mathematics education's past and present state in the United States, emphasizing mathematics intervention instruction. This dissertation includes a complex and critical analysis of intervention practices and historical and structural inequities based on race present in these interventions and mathematics education. In combination, it examines the construct of mathematics identities, their role in student success with mathematics, and trends surrounding mathematics education and its impact on populations of students of color. Using Critical Race Theory as an underlying framework and Critical Counter-Narrative as a methodology, it argues a need for marginalized student voices to be present in the …

Food Insecurity And Residential Segregation Among Adults In The United States: The National Health And Nutrition Examination Survey 2017- March 2020 Pre-Pandemic Data., Chandre' L. Chaney

Electronic Theses and Dissertations

Food insecurity is when a household has insufficient food supply due to limited economic resources. It is a public health issue that continues to persist. The health, social, and economic impact affects millions of people nationwide. Residential segregation is a primary cause of inequities and health disparities. It shapes the differences in socio-economic conditions between Blacks and Whites living in the U.S. This country is segregated across racial lines in many of our most populated metropolitan cities. Americans worship in different churches, learn in disparate schools, and live in separate neighborhoods. Residential segregation has consequences that impact the economy, professional …

School-Wide Academic Outcomes And Instructional Modality Used During The 2018-2022 School Years, Matthew Bowser

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine if there was a significant relationship between school-wide academic outcomes and the instructional modalities utilized by public schools during the 2019-2022 school years. This study also determined if there was a significant difference in assessment scores earned by public school students in Tennessee before and after the shift from in-person schooling caused by the COVID-19 Pandemic. The researcher used a chi-squared test for independence to determine if there was a significant relationship between the modality of instruction used within a school during the 2020-2021 and 2021-2022 school years and the school-wide …

Mapping The Binding Site Within Integrin D2 For Carboxyethylpyrrole (Cep)-Modified Proteins, Afia Prema

Electronic Theses and Dissertations

Neutrophils and macrophages accumulate at sites of inflammation and cause chronic inflammation leading to various diseases. Therefore, to better understand chronic disease pathways it is important to investigate the properties of macrophage accumulation in inflamed tissues. The I-domain of the macrophage receptor integrin a_Db₂ plays a vital role in macrophage retention by binding to CEP (carboxyethyl pyrrole), a ligand available at inflammatory sites. This thesis mainly focuses on evaluating the binding site within integrin a_Db₂ that binds carboxyethyl pyrrole (CEP)-modified proteins. So, a recombinant plasmid construct containing the integrin I-domain was developed. Seven non-conserved …

Strong As A Mother: The Resilience Of Women Who Have Previously Experienced Infertility, Madison Hinkle

Electronic Theses and Dissertations

Infertility impacts numerous individuals during their reproductive journey. Yet, there is little research and information available that examines if having a history of infertility goes on to have further implications after an individual conceives and gives birth. Thus, the current study aimed to examine if infertility impacts the postpartum period, and if there were any particular risk or resiliency factors that contributed to this relationship. In this study, women aged 18 to 50, who had given birth within the last 12 months, participated in an online survey that assessed a variety of physical and mental health constructs. Overall, results largely …

Acute Responses To Combined Accentuated Eccentric Loading And Rest Redistribution, Sungwon Chae

Electronic Theses and Dissertations

This dissertation was 2 parts of investigations of various acute responses to high-volume combined accentuated eccentric loading and rest redistribution (AEL + RR). Resistance-trained men (n = 12, 25.6 ± 4.4 years, 1.77 ± 0.06 m, 81.7 ± 11.4 kg) completed a back squat (BS) 1 repetition maximum (1RM) and weight releaser familiarization session. Three BS exercise conditions (sets × repetitions × eccentric/concentric loading) comprised AEL + RR 5: 3 × (5 × 2) × 110/60%, AEL + RR 2: 3 × (2 × 5) × 110/60%, and traditional sets (TS): 3 × (1 × 10) × 60/60% 1RM. The …

Impacts Of Urbanization On Pollination Success For Common Milkweed (Asclepias Syriaca), David Rockow

Electronic Theses and Dissertations

Urbanization is among the greatest threats to biodiversity on the planet, and is projected to increase in magnitude in coming years. This growing threat makes it important to better understand how urbanization may impact vital ecosystem services, such as pollination. Studies on the impact of urbanization on pollination vary wildly in their findings. This study analyzed multiple aspects of pollination success in common milkweed populations varying in urbanization level, including insect community composition, pollen removal/deposition, and fruit/seed production. By analyzing multiple pollination aspects we can determine the overall mechanism in which urbanization instills on the entire pollination process. Overall, urbanization …

Behavioral Indicators Of Reflective Functioning In Mother-Child Dyadic Interactions, Rachel Clingensmith

Electronic Theses and Dissertations

Positive parenting practices and secure attachments are consistently linked to healthy child outcomes (Ainsworth & Bowlby, 1991; Waters et al., 2000). Research on cognitive processes that scaffold parental behaviors which contribute to secure attachment is an essential contribution to the literature, particularly given the potential for early intervention with at-risk families. Parental Reflective Functioning (PRF) is a construct of increasing interest which has been linked to secure attachments and positive child outcomes, with one commonly used self-report measure of PRF being the Parental Reflective Functioning Questionnaire (PRFQ; Camoirano, 2017; Clingensmith, 2021; Luyten et al., 2017). As such, the purpose of …

Comparison Of The 2022 Monkeypox (Mpox) Outbreak Using Mathematical Modeling And Time Series Clustering, Mark-Daniels Tamakloe

Electronic Theses and Dissertations

Monkeypox virus (MPXV) is the causative agent of monkeypox (mpox), a rare viral disease that affects humans [1]. It is primarily found in Africa and is transmitted to humans through contact with sick animals, particularly rodents and monkeys, or through human-to-human transmission [2]. From the beginning of May 2022, cases of mpox have been recorded from non-endemic nations, and the illness has continued to be reported in other endemic nations. Majority of confirmed cases have been recorded in Europe and North America. In this thesis, we compare the spread of the outbreak across the top ten countries using a combination …

Tennessee Promise And Two-Year Community College Retention And Graduation In Rural Appalachia, Tammy Dycus

Electronic Theses and Dissertations

The purpose of this quantitative, non-experimental study was to explore the relationship between the implementation of the Tennessee Promise scholarship program and the two-year Tennessee community college retention rates and graduation rates of first-time, full-time Tennessee students from rural Appalachian counties. Results from this study may help higher education stake-holders better understand the features of Tennessee Promise that are influencing an increase in community college retention and graduation rates for Tennessee students from rural Appalachian counties. The theoretical framework that guided this research was the social capital framework. Data including use of Tennessee Promise, county of origin, retention, and graduation …

Investigation Of The Protein Components In A Periplasmic Mechanism Regulating Bacterial Morphology, Alexandra Pulliam

Electronic Theses and Dissertations

Salmonella is a leading bacterial cause of foodborne illness worldwide. During a previous study investigating the enzymes responsible for regulating cyclic-di-GMP concentrations, a mutant in the cyclic-di-GMP-specific phosphodiesterase STM3615 was identified that displayed a phenotype characterized by decreased survival on agar plates and a shorter bacterium length. I was able to determine that the periplasmic domain of STM3615 was responsible for this phenotype, not the enzymatic phosphodiesterase domain. Based upon a bioinformatic analysis of the protein, I then hypothesized that the periplasmic domain of STM3615 was interacting with a periplasmic protein to give rise to this phenotype. To identify this …

Extraction, Purification, And Characterization Of Potential Bioactive Compounds Produced By Janthinobacterium Lividum Tajx1901, Andy Elorm Agbakpo

Electronic Theses and Dissertations

Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel compounds. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). The isolated soil bacterium was successfully cultured on rich media agar plates, followed by extraction using methanol and chloroform. The purification methods utilized include flash column chromatography, preparative thin-layer chromatography, and …

Isolation And Structural Determination Of Bioactive Metabolites From The Soil Bacterium, Arthrobacter Sp. Tajx1902, Amonah Arije

Electronic Theses and Dissertations

As antimicrobial resistance persistently disrupts the treatment of microbial infection, natural product chemistry has played a significant role in identifying novel drugs with novel modes of action critical to getting ahead of resistance. The primary goal of this project is to extract and identify potential novel antimicrobial natural products produced by Arthrobacter sp. Although underexplored, Arthrobacter sps. have been known to produce bioactive compounds with versatility; one such is a depsipeptide with quorum-sensing inhibitory activity.¹ In this research, an Arthrobacter sp. TAJX1902, isolated from a soil sample, showed inhibitory activity against a filamentous indicator bacterium and a violacein-producing Janthinobacterium …

Microsporidian Spores And The Integrin Binding Loop Of The Madam Protein Are Important For Integrin Signaling And Attachment To Host Cells, Cindy L. Barrett

Electronic Theses and Dissertations

Microsporidia are a distant fungal pathogen that have severe clinical consequences for the immunocompromised. Previous work identified a microsporidian pathogen protein termed Microsporidian ADAM or MADAM. This protein has close sequence homology to other ADAM proteins (A Disintegrin and Metalloproteinase) in two microsporidian species, Encephalitozoon intestinalis and E. cuniculi. ADAM proteins have a wide range of functions, including binding to integrins and host signaling. It is known that many pathogens manipulate integrins to invade host cells, and it is predicted that microsporidia are also exploiting this host target. Previous work with the MADAM protein demonstrated that this protein has a …

Overdose Prevention Behaviors And The Rural Risk Environment Among People Who Inject Drugs In Rural Appalachia, Jody Teel

Electronic Theses and Dissertations

Introduction: The entire United States has felt the effects of the overdose epidemic, but rural Appalachia has experienced disproportionate overdose deaths. This variation in overdose mortality can be attributed to the risk environment for overdose in rural Appalachia. Overdose prevention behaviors are individual level behaviors that people who inject drugs can implement that may have the potential to reduce their risk of overdose, however limited research exists regarding the utilization of those behaviors. It is important to consider how the risk environment can influence the utilization of overdose prevention behaviors among people who inject drugs. Therefore, the purpose of this …