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Characterization Of A 30s Ribsomal Subunit Intermediate Found In Escherichia Coli Cells Growing With Neomycin And Paromomycin., Cerrone Renee Foster

Electronic Theses and Dissertations

The bacterial ribosome is a target for inhibition by numerous antibiotics. Neomycin and paromomycin are aminoglycoside antibiotics that specifically stimulate the misreading of mRNA by binding to the decoding site of 16S rRNA in the 30S ribosomal subunit. Recent work has shown that both antibiotics also inhibit 30S subunit assembly in Escherichia coli and Staphylococcus aureus cells. This work describes the characteristics of an assembly intermediate produced in E.coli cells grown with neomycin or paromomycin. Antibiotic treatment stimulated the accumulation of a 30S assembly precursor with a sedimentation coefficient of 21S. The particle was able to bind radio labeled antibiotics …

Risk Assessment For Hazardous Materials Incident, Washington County, Tennessee: Road Transportation, Rail Transportation, And Hazardous Materials Storage., Steed Kenlee Stagnolia

Electronic Theses and Dissertations

This study was conducted to provide a quantitative risk assessment for road transportation, railway transportation, and storage of hazardous materials in Washington County, Tennessee. A roadside survey, railroad hazardous materials survey, and a chemical inventory were conducted. It revealed an estimated 267,858 HAZMAT shipments per year on primary and secondary roadways and an estimated total of 9.4 x 109 tons of HAZMAT shipped by rail per year. There are 49 facilities that store a daily average of 33,722,700 lbs of HAZMAT. The risk of a hazardous materials incident occurring in Washington County is highest for Interstate transportation. The risk for …

Confidence Intervals For Population Size In A Capture-Recapture Problem., Xiao Zhang

Electronic Theses and Dissertations

In a single capture-recapture problem, two new Wilson methods for interval estimation of population size are derived. Classical Chapman interval, Wilson and Wilson-cc intervals are examined and compared in terms of their expected interval width and exact coverage properties in two models. The new approach performs better than the Chapman in each model. Bayesian analysis also gives a different way to estimate population size.

The Effect Of Caffeine And Ethanol On Flatworm Regeneration., Erica Leighanne Collins

Electronic Theses and Dissertations

Flatworms, or planarian, have a high potential for regeneration and have been used as a model to investigate regeneration and stem cell biology for over a century. Chemicals, temperature, and seasonal factors can influence planarian regeneration. Caffeine and ethanol are two widely used drugs and their effect on flatworm regeneration was evaluated in this experiment. Non-toxic levels of caffeine, a stimulant, and ethanol, a depressant, were determined. The tails of the flatworms were removed and the regeneration stage was analyzed every 3 days for 15 days to see the effect of these drugs alone and in combination on regeneration. For …

Characterization Of The Group Ii Intron Gs. Int1 From The Thermophilic Bacterium Geobacillus Stearothermophilus., Huijing Sun

Electronic Theses and Dissertations

Group II Introns are small segments of DNA that reside in the chromosome of bacteria or the organelles of primitive eukaryotes. These elements have some very interesting properties. First, they are retrotransposons that can move from one location to a new location in DNA via a reverse transcription mechanism. Second, they form a large ribozyme that mediates self-splicing of the intron from pre-mRNA. A Group II Intron type protein with similarity to reverse transcriptase was discovered in the thermophilic bacterium Geobacillus stearothermophilus strain 10 (Vellore et al., 2004, Appl. Environ. Microbiol. 70: 7140-7147). Numerous copies of the intron, …

The Effects Of Movement On Literacy., Kathy S. Luppe

Electronic Theses and Dissertations

The purpose of this study was to extend the knowledge of the relationship between movement and cognition by examining the impact of a sensory-motor program on the literacy skills of first grade learners from one elementary school in East Tennessee.

Literacy skills were evaluated using five subtests of the Dynamic Indicators of Basic Early Literacy Skills (DIBELS) assessment.

A 2 x 2 x 2 mixed factorial MANOVA was used to analyze group, gender, and test time effects on multiple dependent variables. The analysis yielded a statistically significant result (p < .05) in gains for the movement group and a …

Adolescents And Marijuana Use: The Affects Of Peer And Parent Relationships And Substance Abuse Education., Samuel Joseph Cosimano

Electronic Theses and Dissertations

The purpose of this study was to analyze gender, race, substance abuse programs such as Drug Abuse Resistance Education (D.A.R.E.), parents, and peers and their ability to influence or predict adolescents and their decisions to use marijuana. All of the variables used for this study came from secondhand data collected by Esbensen and Osgood (1999), Gang Resistance Education and Training (G.R.E.A.T.). The analysis revealed that males are more likely to have ever used marijuana, that mixed race adolescents have a higher rate than other races to have ever used marijuana, that when adolescents complete the substance abuse program, D.A.R.E. have …

A Comparison Of The Academic Achievements Of Intermediate Students Based On Socioeconomic Status And Participation In An After-School Program., Anthony Fayne Maxwell

Electronic Theses and Dissertations

The purpose of this study was to determine what, if any, associations exist between students' academic success on achievement tests and the predictor variables of students' socioeconomic status, participation in an after-school program, and gender. Middlesboro Intermediate School contains a high number of students who participate in the federal free- or reduced-price school meals program, as well as attend the after-school program. This study factored in the student's academic success on the Kentucky Core Content Test and the socioeconomic status of students based on their qualification for the federal free- or reduced-cost school meals program, their participation in the after-school …

The Impact And Efficacy Of Diabetes Education Programs Among Adults., Mireille Hamdan

Electronic Theses and Dissertations

The purpose of this study was to measure the impact of diabetes education classes in increasing knowledge and promoting healthy lifestyle behaviors. Thirty-three subjects participated in the classes between October 2004 and October 2005. Only six subjects agreed to participate in this study. Changes in knowledge after the classes were measured by a survey test one year following the completion of classes. Survey responses were analyzed using percentages. Subject's Hgb A1C and weights were also collected to measure the direct impact of education on participants' blood glucose management. Overall, participants were very knowledgeable of diabetes symptoms, complications, carbohydrate counting, and …

Evaluation Of An Elementary School Wellness Concept In Rural East Tennessee., Audrey Lynn Taylor

Electronic Theses and Dissertations

Parents of elementary school children in the small, rural area of Unicoi County, TN were surveyed to determine their attitudes toward health, nutrition, and Unicoi County Schools' Wellness Policy. Elementary school classrooms were randomly chosen to receive surveys for the children's parents to return by mail. Data were compiled and analyzed using SPSS software. Over 99% of parents stated that nutrition education in schools was important, and 96% stated schools played an important role in their child's health. The assessment provided meaningful data and laid groundwork for future nutrition education programs. The research showed rural, lower-income parents are supportive of …

The Effects Of Nicotine Conditioned Place Preference In D2 Primed Adolescent Rats: Age-Related And Gender Effects., Yoko Emily Ogawa

Electronic Theses and Dissertations

This study investigated nicotine conditioned place preference (CPP) in two different ages of adolescence using a rodent model of schizophrenia. Both 2- and 3-chambered CPP apparatuses were used to test whether the CPP was due to an aversion to the white chamber. Animals were neontally treated with the dopamine D₂/D₃ agonist, quinpirole, or saline and raised to either early postweanling age (P 22) or adolescence (P 29). Rats were conditioned to prefer the white chamber using nicotine. Results showed that nicotine induced CPP and appeared to alleviate an increased stress response in D₂ primed animals, which …

Beverage Consumption And Hypertension: Findings From The Third National Health And Nutrition Examination Survey., Vishal Arunbhai Mandge

Electronic Theses and Dissertations

The study sample was comprised of 18,953 subjects aged 18 years and older who participated in the Third National Health and Nutrition Examination Survey. Preference for diet soda over regular soda was higher in females as compared to males and in Caucasians as compared to other races. Mean alcohol consumption was almost three times higher in males than in females. Undiagnosed hypertension was more common in males than in females, in 65-90 than in 50-64 year olds, and in people with less than high school education compared to those with higher education. Diastolic pressure was correlated with the level of …

An Evolving Change In Public Schools: An Assessment Of Teachers' And Administrators' Perceptions And Classroom Changes Concerning High-Stakes Testing., Selena Marie Kiser

Electronic Theses and Dissertations

The intent of this study was to investigate changes in the curriculum caused by high-stakes testing mandates within 3 Southwest Virginia school systems to find best practices for instructional application in classrooms. This qualitative study was comprised of indepth interviews and observations with elementary school teachers and administrators. High-stakes testing has impacted the nation in myriad ways. The mandates from the government presented teachers and administrators with conditions that must be met according to the No Child Left Behind Act and individual state's standards. Teachers' perceptions of curriculum changes, Developmentally Appropriate Practices (DAP), testing mandates according to high-stakes testing, and …

An Assessment Of Read 180 Regarding Its Association With The Academic Achievement Of At-Risk Students In Sevier County Schools., Jayson Nave

Electronic Theses and Dissertations

READ 180 is an intensive reading intervention program designed to meet the needs of students whose reading achievement is below the proficient level. The program addresses individual learning styles through adaptive software, interesting literature, and direct instruction with reading skills. The purpose of this study was to compare the achievement of academically at-risk students in Sevier County Public Schools in East Tennessee who participated in the READ 180 pilot program with the achievement of their academically at-risk peers not enrolled in the intervention program before and after its implementation in order to assess the reading intervention program. The Sevier County …

The Development Of The Self-Injury Self-Report Measure., Sonia Lorraine Coney

Electronic Theses and Dissertations

Despite the amount of research that has been conducted on self-injury there is a lack of empirically validated instruments with which to measure self-injurious behavior. The present study developed a measure to examine self-injury and the associated features. Undergraduate students (n = 184) were administered a set of surveys to assess demographics, self-injurious behavior, suicidal ideation, Axis I and Axis II disorders, and impulsivity. Results indicated that a reliable measure, able to assess the extent of self-injury as well as associated features, was developed. Such a measure will enable clinicians to better assess self-injury and enable researchers to more fully …

A Study Of Perceptions Of Achievement Factors For At-Risk Students In Comparison To Honor Students At A Northeast Tennessee High School., Christopher Michael Hampton

Electronic Theses and Dissertations

Student success and motivation are issues that baffle parents and educators. Various factors go a long way in explaining why these educational phenomena occur. Current research has confirmed that issues such as parental involvement, home life, attitude, intellect, and teacher and student relations and rapport have an impact on motivation and future success of students. This research project focused on students' perceptions of factors that affect their success and achievement. These factors were categorized into four areas: intrapersonal influences, parental influences, educational influences, and external influences. These areas were explored in an attempt to promote an awareness of the factors …

Forecasting Prescription Of Medications And Cost Analysis Using Time Series., Mussie Angesom Tesfamicael 1975-

Electronic Theses and Dissertations

The purpose of this research study is to examine the use of time series forecasting and text mining to investigate the prescription of antibiotics. The specific objective is to examine the relationship between the total payments, private insurance payments, Medicare payments, Medicaid payments, number of prescriptions and quantity of prescriptions for different antibiotics. Currently, there is no method available to forecast antibiotic prescription costs, so we have adopted several methods that will help health care providers and hospitals to know about the prescription of the antibiotics being prescribed. The payment made for each antibiotic is based upon an average cost …

A Study Of Relationships Of School Climate, School Culture, Teacher Efficacy, Collective Efficacy, Teacher Job Satisfaction And Intent To Turnover In The Context Of Year-Round Education Calendars., Joseph W. Mattingly 1953-

Electronic Theses and Dissertations

The goal of this study was to investigate the relationships between school climate, school culture, teacher efficacy, collective efficacy, teacher job satisfaction and intent to turnover in the context of year-round education (YRE) calendars. The research design of this study utilized an e-mail invitation to participate with a uniform resource locator (URL) embedded in the message to link participants to an internet-based questionnaire. The questionnaire was delivered to 1,254 teachers employed in nine participating school districts in Kentucky that operated on YRE school calendars. The response rate was 60%. Teachers ( N = 748) responded to an internet-based questionnaire consisting …

A Theory-Based Intervention To Increase Dental Utilization By Disadvantaged Children., Catherine J. Binkley

Electronic Theses and Dissertations

Oral disease is the greatest unmet healthcare need of disadvantaged children in the United States, with only 20% of disadvantaged Medicaid-eligible children receiving routine dental care. Peter Margolis proposed a theoretical model of access to healthcare services and reported the effectiveness of a case manager intervention in increasing medical care utilization by disadvantaged mothers and infants. This study was based on Margolis's theory and used a case manager to assist parents in obtaining dental care. Methods. The study was based on secondary analyses of data from the "Parental Help Seeking for Dental Care" project. The randomized, controlled intervention study was …