Digital Commons Network^™

Understanding Human Gut Bacterial Interactions And Their Role In Colonization Resistance Against Clostridioides Difficile Using Mini-Bioreactor Array And Caenorhabditis Elegans As Model Systems, Achuthan Ambat

Electronic Theses and Dissertations

No abstract provided.

Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir

Electronic Theses and Dissertations

Pyraclostrobin, a QoI fungicide, is extensively used in the management of fungal leaf spot diseases. In this study, 215 P. tritici-repentis isolates collected from wheat fields in South Dakota were evaluated for their sensitivity to Pyraclostrobin. Of 215 isolates, 48 isolates (22%) exhibited insensitivity based on G143A mutation and EC50 values using spore germination assay. Our results indicate reduced sensitivity to insensitivity to pyraclostrobin in some isolates suggesting regular monitoring of the P. tritici-repentis population to QoI fungicides is essential to track the evolution of insensitive population. Spelt wheat (Triticum spelta L.) is an ancient cereal that is gaining small …

U.S. Consumers’ Willingness To Pay For Raised Carbon-Friendly Beef: Moderation Effect Of Information And Consumer Characteristics, Mariam Ishaq

Electronic Theses and Dissertations

No abstract provided.

Development Of Corn Kernel-Based Biocomposite Films For Food Packaging Applications, Swastika Bera

Electronic Theses and Dissertations

Most of the current and active food packaging resources and methods are nonbiodegradable and nonrenewable therefore harmful to the environment. Due to this, alternate sources of food packaging materials are in high demand. In this study, a bio-composite film has been developed, with Corn kernel powder as fiber reinforcement which is mixed with gelatin, and lignin two biopolymers as the matrix. The effect of Corn Kernel (CK) reinforcement on the Gelatin/Lignin (G/L) matrix on mechanical and barrier properties has been studied. CK has shown great potential as reinforcement to natural polymer, gelatin, and lignin (G/L) for food packaging applications as …

Effects Of Dietary Genistein And Fugacity Modelling In An Aquaculture System, Travis Schaeffer

Electronic Theses and Dissertations

Fish production from aquaculture has continued to grow over the past several decades, with finfishes being fed formulated, fish meal-based diets supporting most of that industry’s growth. However, increased demand and costs, coupled with static volume and erratic supplies of fish meal, has resulted in the use of alternative plant-derived protein sources. A common oilseed protein source is soybean meal (SBM), because of its protein content and amino acid profile; however, it also contains multiple anti-nutritional factors, including phytoestrogens. Phytoestrogens are naturally produced, estrogenmimicking compounds, with isoflavones receiving the most investigation as higher levels of these compounds occur within SBM. …

Identification And Quantification Of Novel Antimicrobials, Hazera Khatun Koly

Electronic Theses and Dissertations

No abstract provided.

Assessments Of Water Chemistry And Fish Community Structure In The Buffalo Creek Watershed, Tributary To The Allegheny River In Armstrong And Butler Counties, Pennsylvania, Kathleen Wilson

Electronic Theses and Dissertations

The Buffalo Creek watershed has experienced water quality impairments for various reasons, including but not limited to, agricultural influence, abandoned mine drainage (AMD), on-site wastewater malfunction, and stormwater. The source and cause of the most prevalent impaired areas remains unknown. The goal of this study was to analyze water chemistry using state water quality standards and mass ratio analysis, then to conduct fish community analysis. We found toxic metal pollution across the watershed, with iron, cadmium, copper, and selenium frequently exceeding criteria. We determined which streams are polluted by resource extraction activities by conducting mass ratio analysis. We found conventional …

The Ethical Imperative Of Inclusion In Healthcare Innovation: Scaling And Sustaining Digital Health Equity, Lukas E. Chandler

Electronic Theses and Dissertations

The COVID-19 pandemic revealed the harm of digital health exclusion. Excluded groups could not benefit from virtual care due to device access gaps, broadband connectivity, and digital and language literacy levels. A lack of engagement with historically excluded populations in digital health innovation is an ethical harm and a maleficent form of social exclusion. Digital tools in healthcare delivery represent more than just a strategy for promoting health equity; they signify an opportunity to address health inequities in the health technology design process.

The historical focus of healthcare ethics on bedside decision-making issues largely explains why the field lacks concerted …

A Qualitative Study Into Modern Psychoanalytic Joining And Mirroring Through Psychotherapeutic Exchanges, Tricia Shihui Wang

Electronic Theses and Dissertations

This dissertation intends to address current deficits in knowledge regarding the modern psychoanalytic interventions of joining and mirroring, including substantial definition issues in the literature and the absence of current writings on the psychotherapeutic interventions of joining and mirroring in practice. The focus of the study was on the ever-dynamic landscape between modern analytically-informed practitioners and their clients in psychotherapeutic exchange(s) that the practitioners believed encompassed joining and/or mirroring interventions. Participants were six experienced practitioners who described their clinical work as being informed by modern psychoanalysis and who have engaged or were currently engaging in individual psychotherapy with a client. …

Redefining Safety: A Reproductive Justice Ethics Of Care Approach To Maternal Health, Elizabeth Balskus

Electronic Theses and Dissertations

This dissertation will argue for the adoption of a multi-dimensional concept of safety in healthcare to include emotional, psychological and communal health. It will then illustrate how this concept of safety is foundational to a dignity-enhancing ethics of care approach that finds its normative grounding in Reproductive Justice and human capabilities, given that both patient and provider need to feel safe in order to fully engage in a dialogical-interpretive process of care and that caring efforts must strive to be equitable on both interpersonal and structural levels. This framework will then be applied to maternal health, showing how an approach …

Implications Of Individuals With Music Learning Experience Using A Segmented Multimedia Lesson In A Non-Music Discipline, Cheryl Farren Tkacs

Electronic Theses and Dissertations

The purpose of this study was to investigate how prior music learning experience relates to learning with multimedia resources in non-music disciplines. Specifically, whether years of music learning experience, type of music learning experience, or type of multimedia were associated with task scores and task duration in a non-music discipline. The study revealed the transferable benefits of music learning experience to learning outcomes in other disciplines. Encouraging music studies as part of the base curriculum is thus strongly encouraged. However, as years of music learning and the presence or absence of segmenting in multimedia learning resources were not found to …

Tabletop Role-Playing Games As Autism Psychotherapy: A Video-Cued Multivocal Clinical Ethnography, Adina Rubin-Budick

Electronic Theses and Dissertations

This qualitative study is a video-cued multivocal ethnography that analyzed the clinical reasoning and perspectives of psychotherapists using Tabletop Role-Playing Games (TRPG) as a therapeutic treatment for children and adolescents diagnosed with Autism Spectrum conditions. With a growing number of TRPG therapists nationwide using this modality in their clinical practices, this study aimed to develop a clearer understanding of the ways in which their therapeutic orientation, gaming practices, and knowledge of Autism Spectrum Conditions informs their use of TRPG psychotherapy with Autistic clients.

Research that focuses on clinical decision-making and the knowledge that informs clinical interventions and technique generally refers …

Situating Heirloom Presence Within The Family Narrative: That Is/Was Then, This Is/Was Now, Rachel Savorelli

Electronic Theses and Dissertations

Intramundane objects often become “intergenerational transfers of contaminated objects,” commonly known as heirlooms (Belk 151; Heisley and Cours 425; Holmes). Rather than priceless possessions, heirlooms are mostly ordinary objects, such as photographs and gardening tools. Regardless of rarity or monetary value, as familial artifacts, heirlooms compel an ethic of responsibility. For this project, the term “heirlooming” is a performance of communicative practices of story-telling, ritualizing, and kin-keeping that contribute to heirloom preservation and continuation. A narrative ethics background structures family communication to both make meaning and make sense of ongoing and changing practices across generations. Heirlooms situated within family narratives …

Microplastic Accumulation In The Digestive Tract Of Young-Of-Year Atlantic Sharpnose Sharks (Rhizoprionodon Terraenovae) In The Grand Strand, Sc, Andrew Curtis Sitlinger

Electronic Theses and Dissertations

This study focused on the presence and accumulation of microplastic fibers in the digestive tract and livers of young-of-year Atlantic Sharpnose Sharks (Rhizopriondon terraenovae) from two sampling locations along the Grand Strand of South Carolina. R. terraenovae is a small, mesopredatory elasmobranch found abundantly along northwestern Atlantic Ocean coastlines. Thirty specimens of R. terraenovae were collected from May through August of 2020. Microplastics were found in all specimens. A total of 672 plastic particles were identified over the course of the study, with an average of 22.4 ± 10.5 (SD) plastics per specimen. The majority of the plastics were classified …

Fine-Scale Site Selection Of Strand-Feeding Common Bottlenose Dolphins (Tursiops Truncatus) And Their Prey, Deborah Kleinclaus

Electronic Theses and Dissertations

Strand-feeding is a unique, cooperative foraging strategy used by some common bottlenose dolphins (Tursiops truncatus) at low tide in South Carolina and Georgia salt marshes, where a group of dolphins charges schools of fish, thrusting them onto the bank with their bow wave and then capturing prey items onshore. Strand-feeding dolphins are estuarine residents and frequent predictable stranding sites, suggesting an intimate knowledge of the area. Dolphin behavior prior to stranding suggests they do not herd the fish before charging, but instead prey on fish already shoaling near the bank. The use of active acoustics from strand-feeders in order to …

Death Of A Bacterium: Exploring The Inhibition Of Staphylococcus Aureus By Burkholderia Cenocepacia., Tiffany Brandt

Electronic Theses and Dissertations

Antimicrobial resistance is a phenomenon of increasing concern as antimicrobial overuse and misuse eliminate current therapeutic options, ushering society into a post-antimicrobial era. Antibiotic discovery and synthesis efforts are urgently needed to counter the increasing burden of antimicrobial resistance. Staphylococcus aureus is a causative agent of a variety of clinical manifestations including bacteremia, endocarditis, soft tissue infection, osteomyelitis, and device-related infections. S. aureus infection presents additional treatment challenges due to its capacity for biofilm formation, which is a mode of growth that confers protection from antibiotics and physical elimination, and the emergence of antibiotic resistant strains, including methicillin-resistant S. aureus …

Understanding Neuroanatomy In A Virtual 3d Environment: Creation And Use Of A New Survey Tool To Evaluate The Effectiveness Of 3d Software In Neuroanatomy Education For Understanding Superficial And Deep Brain Structures., Akash Khare

Electronic Theses and Dissertations

Studying cross-sections is a critical approach to learning and testing knowledge in neuroanatomy and the role of 3D technologies have been gradually increasing in medical education, especially after the COVID-19 pandemic. A study was conducted in a quasi-experimental one-group pre-post interventional design in an online setting by creating and evaluating the effectiveness of a virtual lab in neuroanatomy for all neuroscience students enrolled in the Fundamentals of Neuroscience course in our department at the University of Louisville. Study modules were created using the 2D resources used in previous years and 3D web applications of Visible Body and AnatomyLearning.com software. A …

Design, Evaluation, And Control Of Nexus: A Multiscale Additive Manufacturing Platform With Integrated 3d Printing And Robotic Assembly., Danming Wei

Electronic Theses and Dissertations

Additive manufacturing (AM) technology is an emerging approach to creating three-dimensional (3D) objects and has seen numerous applications in medical implants, transportation, aerospace, energy, consumer products, etc. Compared with manufacturing by forming and machining, additive manufacturing techniques provide more rapid, economical, efficient, reliable, and complex manufacturing processes. However, additive manufacturing also has limitations on print strength and dimensional tolerance, while traditional additive manufacturing hardware platforms for 3D printing have limited flexibility. In particular, part geometry and materials are limited to most 3D printing hardware. In addition, for multiscale and complex products, samples must be printed, fabricated, and transferred among different …

The Impact Of Volatile Organic Compound Exposure On Subclinical Biomarkers Of Cardiovascular Injury., Breandon Taylor

Electronic Theses and Dissertations

Pollution has been identified as the leading environmental cause of non-communicable disease and premature deaths globally. Volatile organic compounds (VOCs) are gaseous chemical constituents of pollution derived from a variety of sources, including industrial solvents and byproducts, automobile exhaust, tobacco smoke, cleaning supplies, and personal care products. VOCs are also abundant at various Superfund and Hazardous Waste Sites. Emerging data suggest that VOC exposure is associated with several adverse health outcomes, including cardiovascular disease (CVD). VOCs and their metabolites can potentially damage the endothelial lining of blood vessels, resulting in perturbed vascular function and vascular inflammation. We hypothesize that VOC …

Investigation Of Human N-Acetyltransferases (Nat1 And Nat2) Genetic Polymorphisms In Susceptibility To Aromatic Amine And Alkylaniline Genotoxicity., Mariam Habil

Electronic Theses and Dissertations

Arylamine N-acetyltransferases, NAT1 and NAT2, catalyze the detoxification and/or activation of carcinogens. Single nucleotide polymorphisms or SNPs result in different human NAT1 and NAT2 genotypes. We hypothesize that different NATs alleles will impact levels of N-acetylation, HPRT mutations, DNA damage and oxidative stress induced by carcinogens. NER-deficient Chinese hamster ovary (CHO) cells transfected with human CYP1A2 and NAT1*4, NAT1*14B, NAT2*4, NAT2*5B or NAT2*7B have been used to investigate N-acetylation of benzidine, β-naphthylamine (BNA), and 4, 4’-methylene bis (2-chloroaniline) (MOCA) as aromatic amines and 3,4-dimethylaniline (3,4-DMA) as one of alkylanilines and their associated toxicity. Our …

A New Direction For Public Understanding Of Science: Toward A Participant-Centered Model Of Science Engagement., Christopher Rickels

Electronic Theses and Dissertations

Engaging the public with science is not an easy task. When presented, scientific findings, public health recommendations, and other scientific information filter through the personal values, beliefs, and biases of members of the public. Science communicators must contend with these differences in order to be effective in cultivating a public understanding of science. Given the importance of scientific understanding for living well in a complex world, increasing science understanding through science engagement is imperative. The field of public engagement with science is dichotomized by a public information deficit approach and a contextualist approach. The deficit approach prizes the factual content …

Metabolic Foundations Of Exercise-Induced Cardiac Growth., Kyle Fulghum

Electronic Theses and Dissertations

Regular aerobic exercise promotes physiological cardiac growth, which is an adaptive response thought to enable the heart to meet higher physical demands. Cardiac growth involves coordination of catabolic and anabolic activities to support ATP generation, macromolecule biosynthesis, and myocyte hypertrophy. Although previous studies suggest that exercise-induced reductions in cardiac glycolysis are critical for physiological myocyte hypertrophy, it remains unclear how exercise influences the many interlinked pathways of metabolism that support adaptive remodeling of the heart. In this thesis project, we tested the general hypothesis that aerobic exercise promotes physiological cardiac growth by coordinating myocardial metabolism to promote glucose-supported anabolic pathway …

The Impact Of Pegylation On Cellular Uptake And In Vivo Biodistribution Of Gold Nanoparticle Mri Contrast Agents., Nagwa El-Baz

Electronic Theses and Dissertations

Gold nanoparticles (GNPs) have become a pivotal platform for the delivery of pharmaceutical and diagnostic agents as well as for general therapeutic purposes. Despite their potential for use in biomedicine, their interaction with serum proteins is crucial as it could change their biological profile due to the formation of a protein corona, which can affect their delivery to target tissues in the body. Grafting the GNPs with polyethylene glycol (PEG) is widely used in research to decrease opsonization of the particles by serum proteins and decrease the uptake by the reticuloendothelial system. Here in this dissertation, we have developed a …

Defining The Impact Of Clinically Modeled Hindlimb Stretching, Exercise, & Inactivity On Functional Recovery After Spinal Cord Injury., Greta Cesarz

Electronic Theses and Dissertations

Spinal cord injury (SCI) is a devastating, life altering event that affects approximately 282,000 Americans. The most obvious side effect of SCI is paralysis due to damage to the spinal cord that disrupts ascending and descending pathways as well as central pattern generating circuitry. In addition to paralysis, patients suffer from other debilitating side effects including altered cardiovascular function, autonomic dysreflexia, neuropathic pain, spasticity, and contractures. In contrast to humans, rodents display spontaneous locomotor recovery following incomplete SCI due to in-cage activity/training. Previously, our laboratory has studied the effect of lack of in-cage training by utilizing custom designed rodent wheelchairs. …

Cadence And Range Of Motion Modulate Pedal Force In A Rat Model Of Motorized Cycling After Spinal Cord Injury., Gregory States

Electronic Theses and Dissertations

Motorized cycling (MC) can be utilized post-spinal cord injury (SCI) in patients who lack the strength and/or stability to participate in traditional physical exercise interventions. MC has been applied with the goal of improving locomotor function or cardiovascular health in both human and animal models of SCI. However, a discrepancy exists between the results of human and animal studies of MC, particularly regarding cardiovascular outcomes. Despite the abundance of studies in both humans and animals, the mechanism behind the improvements in cardiovascular function following MC are poorly understood. We posited that increased venous return during MC is likely due to …

Re-Engineering Cardio-Oncology Testing Using Biomimetic Heart Slice Cultures., Jessica Miller

Electronic Theses and Dissertations

28% of drug withdrawal from the market are due to unforeseen disruptions in cardiomyocyte contractility and electrophysiology. The most commonly used platforms for drug testing are in vivo animal models and in vitro cell culture models. While both have been of paramount importance for the discovery and detection of many cardiotoxicities and mechanisms of action, they lack the ability to model an intact human myocardium. This work aims to establish cardiac tissue slices, which are 300-micron thin tissue sections taken from the left ventricular myocardium, as an alternative platform for cardio-oncology studies, specifically cardiotoxicity testing. Additionally, this work aims to …

Sex And The Superman: Gender And The Superhero Monomyth, Christopher Maverick

Electronic Theses and Dissertations

Since the 1938 introduction of Superman, superheroes have been ever-present in American popular culture. Indeed, with the modern preponderance of comic book movies dominating the American cinematic box-office, superhero fantasy is arguably the most important genre of fiction being produced in the contemporary moment. Peter Coogan, Kurt Busiek and many other scholars have discussed the prominence and relevance of the superhero fantasy as a genre. Still others, including Umberto Eco and Marco Arnaudo, have asserted that the superhero is not so much a genre and as it is the evolution of mythology. In Sex and the Superman, I argue …

The Mechanisms Of Mitochondrial Dysfunction In T Cell Aging During Chronic Viral Infection, Madison B. Schank

Electronic Theses and Dissertations

Human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infections induce a myriad of disturbances to CD4 T cell functions, including mitochondrial compromise, excessive inflammation, increased telomeric DNA damage and attrition, cellular exhaustion and senescence, and accelerated aging. In this dissertation, the mechanisms underlying metabolic failure, accelerated aging, and cellular dysfunctions were evaluated in CD4 T cells from healthy subjects (HS) treated with a telomere-targeting drug (KML001) or HCV-infected individuals or people living with HIV (PLHIV) compared to HS. We observed that KML001-induced telomere injury resulted in mitochondrial swelling and decreased mitochondrial membrane potential, cellular respiration, mitochondrial DNA (mtDNA) copy …

Examining The Effects Of Npra Antagonist Anantin On Sci-Induced Ployuria., Lindsey Zipperer

Electronic Theses and Dissertations

Polyuria, or the over production of urine, is a prevalent condition that significantly impacts the quality of life in individuals suffering from a spinal cord injury (SCI). Post-SCI induced polyuria is thought to have been associated with altered levels of vasopressin (AVP), atrial natriuretic peptide (ANP), and natriuretic peptide receptor A (NPRA). In the present study, the function of NPRA was analyzed in the rat contusion model using anantin, an NPRA antagonist. A daily dose of either 100 µg of anantin or vehicle was administered intraperitoneally immediately following the SCI surgery and continued until termination, 4 weeks post injury (wpi). …

Foreign Policy In Media: An Examination Of The Captain America Films., Kat Hernandez

Electronic Theses and Dissertations

Popular culture and media are consumed daily by billions around the world, media which can contain meaningful and politically relevant material. The three Captain America films from the 2010s represent this phenomenon in the presentation of Captain America as a nationalist superhero with geopolitically relevant storylines. The direction and production of these films illustrate significant parallels to foreign policy choices of the United States. Through an interpretive analysis of the Captain America films: Captain America: The First Avenger, Captain America: The Winter Soldier, and Captain America: Civil War, this project seeks to analyze Captain America’s image and …