Digital Commons Network^™

Epicertin: Preclinical Development Of A Novel Mucosal Healing Agent For Ulcerative Colitis., Micaela Reeves

Electronic Theses and Dissertations

This dissertation describes the development of a biotherapeutic agent, EPICERTIN, that fulfills an unmet treatment need for ulcerative colitis (UC): mucosal healing. EPICERTIN is a recombinant cholera toxin B subunit (CTB) variant with a KDEL endoplasmic reticulum (ER) retention motif that is crucial for its wound healing capabilities. Previous studies have demonstrated that EPICERTIN’s mucosal healing activity is due, in part, to its prolonged residence in the ER which initiates an unfolded protein response (UPR) and subsequent TGF-β signaling and barrier repair in colonic epithelial cells. This activity was demonstrated in a dextran sulfate sodium (DSS) colitis model as well …

Impact Of Employee-Supervisor Attachment Styles On The Prevalence And Symptomatic Expression Of Secondary Traumatic Stress In Public Child Welfare Workers., Lisa Purdy

Electronic Theses and Dissertations

Child welfare organizations throughout the nation grapple with turnover rates estimated to be as high as 65% (e.g., CWLA, 2016; Edwards & Wideman, 2018). Turnover contributes negatively to client outcomes, organizational financial shortages, and to perpetuating stressful conditions for the child welfare workforce. One factor found to contribute to turnover within child welfare is that of secondary traumatic stress (STS). Child welfare workers are known to experience higher rates of STS than any other helping professional (Brady, 2017). However, while many risk and protective factors for secondary traumatic stress have been evaluated, there remains a gap in the literature as …

Our Story, Our Song: Assessing And Addressing Black Women's Healthcare Experiences., Jason Deakings

Electronic Theses and Dissertations

Despite advancements in medicine and health sciences, health disparities and declining life expectancy persists among Black women in the United States. Researchers and non-traditional healthcare providers suggest alternative healing (e.g., music and Sister Circles) and research methodologies to achieve a culturally conscious, traumainformed, quality, and holistic care and practice. The intersecting identities and cultural responsibility of Black women highly influences the barriers and facilitators on the uptake of healthcare services. Utilizing a community engaged research approach, this qualitative study assessed the healthcare experiences, as well as the role of music in the lived and retold experiences of Black women navigating …

Fear Of Becoming A Victim Of Crime On A College Campus: A Visual And Factorial Experimental Design Survey Analysis Of Location And Demographic Factors., Nancy Steinmetz

Electronic Theses and Dissertations

Vanessa LoBue (2013) states that the emotion of fear, “a signal of impending threat” (p. 38) is common among mammals. For over five decades, there has been substantial research into what in society, or our communities, makes us fearful. It is this author’s intention to examine how college students’ fear of crime or fear of victimization may be heightened or intensified by specific factors that are commonplace on college campuses and areas adjacent to those campuses. Nicole Rader (2004) argues that the fear of crime discourse needs to be expanded to a larger “construct” called “the threat of victimization” (p. …

Still Got The Blues - No Time But Here, No Place But Now: Geographies Of Art & Activism In The Work Of Clyde Woods., Jake Mace

Electronic Theses and Dissertations

The fight against displacement and dispossession in the increasingly inequitable process of reterritorialization is arguably the most pressing struggle of the modern city – and it is articulated through cultural production as much as through political debate and economic development. Yet research that is at the nexus of critical, urban, and cultural studies and which explores the resistant potential of countercultural production rather than the hegemonic impact of mainstream cultural production is underdeveloped. Clyde Woods’s blues epistemology offers a guide to reading cultural production of marginalized groups as maps of meaning that offer alternative visions of the past, the present, …

A Score Of Voices: Creating And Scoring An Original Film With A Cappella Choir, Zachary James Moore

Electronic Theses and Dissertations

Film scores typically feature large instrumental ensembles, sometimes with choir added at pivotal moments. Rarely, however, is the choir entrusted with a greater percentage of the score, and far too scores explore the sonic potential that vocal music has to offer. In fact, in a recent study of 800 films, none contained a score that was completely c. Thus, the body of unaccompanied choral film scores is practically non-existent. This curious gap in repertoire inspired me to create the documentary Downstream (2023) and score it entirely with an unaccompanied choir. The full score to this new work is contained within …

Neonate Atlantic Sharpnose Shark (Rhizoprionodon Terraenovae) Relative Abundance And Body Condition In Two South Carolina Estuaries Varying In Urbanization, Rileigh E. Hawk

Electronic Theses and Dissertations

Urbanization near estuaries has been shown to affect the growth and survival of juvenile sharks using the system as a nursery. North Inlet and Murrells Inlet, South Carolina, are similarly-sized, tidally-dominated, bar-built estuaries with extensive Sporobolus-lined tidal creeks but differ in degree of human impact. Previously, Murrells Inlet was shown to have a lower abundance and diversity of large sharks than North Inlet and Atlantic Sharpnose Sharks (Rhizoprionodon terraenovae) were shown to use North Inlet as a primary nursery. To examine potential differences in neonate shark abundance and growth between a developed estuary, Murrells Inlet, and a protected estuary, North …

Development Of Three Novel Edna Assays For Detecting Coastal Sharks, Kristina Dawn Flanigan

Electronic Theses and Dissertations

Environmental DNA (eDNA) analysis is an emerging, non-invasive community monitoring tool. This study aimed to determine if eDNA methods can be reliably used in a large brackish, partially mixed estuary by developing and testing three novel eDNA primers, for Sandbar Sharks (Carcharhinus plumbeus), Blacknose Sharks (Carcharhinus acronotus), and Bonnetheads (Sphyrna tiburo). These primers were designed to target 109, 156, and 120 base pair (bp) fragments, respectively, of the highly conserved NAD2 gene in the mitochondrial genome of each species. Primer function was validated through testing against 102 known genomic source samples and 25 filtered water samples from aquaria in which …

Understanding The Persuasive Attributes Of Twitch Advertisements: A Study On The Effects Of Current Advertisements And Sponsorships, Colleen Sharkey

Electronic Theses and Dissertations

The research in this study aims to investigate the effectiveness of Twitch’s current advertising option, which includes mid-roll, pre-roll, and sponsored streams. It will focus on usefulness of content, brand credibility, purchase intent, and streamer credibility. Twitch has seen a surge in growth and attention in recent years, with an average of 31 million daily visitors and 1.3 trillion minutes watched globally in 2021 alone (Twitch Sales, 2022). However, despite Twitch’s growing popularity as an entertainment and entrepreneurship platform, little research has been conducted on the impact of its advertising options on brand perception. This study seeks to address this …

Geophysical, Archaeological, And Geospatial Investigations At Presidio Los Adaes, 18th Century Capital Of Spanish Texas, Robert Linam

Electronic Theses and Dissertations

Presidio Los Adaes (16NA16), located in present day northwest Louisiana, served as the capital of Spanish Texas for much of the 18th century. Maps and historical documents provide architectural plans for the fort and other buildings on the site but differ in the size and location of several buildings. In 2009, a geophysical survey of the site with ground penetrating radar, magnetometry, electrical resistance, and electromagnetic induction showed good preservation of architectural foundations. Visual assessment of the maps and geophysical data, information from archaeological excavations, and spatial statistics suggests that the fort was originally built as specified by the architect, …

Use Of Space And Foraging Behavior Of Loggerhead Shrikes In Urban Areas, Katie Anne Maddox

Electronic Theses and Dissertations

Loggerhead shrikes, Lanius ludovicianus, are a declining grassland songbird native to North America. Land-use change has been associated with the decline of grassland bird species. However, the process of urbanization creates novel short-grass habitats reminiscent of shrikes’ natural habitats. Loggerhead shrikes have been observed living in developed areas, particularly in Southeastern coastal states. Understanding the long-term impacts of urban-dwelling on loggerhead shrike populations is vital to identifying whether urban areas offer refuge or act as an ecological sink for shrike populations.

In chapter one, I quantified the use of space by shrikes in urban habitats within Horry County, South …

Deterrent Effects Of The Select Magnetic And Repellent Treated (Smarttm) Hooks And The Recently Developed "Smarter" Hooks On Sharks In Recreational Hook-And-Line And Longline Trials, Ryan Lowndes

Electronic Theses and Dissertations

As bycatch continues to impact global shark populations, there is a continuing need for effective bycatch reduction devices. Prior research has shown promise in exploiting sharks' electrosensory ability to this end. We tested the deterrent efficacy of the Select Magnetic and Repellant Treated (SMARTTM) and the newly developed "SMARTER" hooks in experimental longline and hook-and-line trials. Both are magnetized and contain an electropositive metal component made of magnesium (SMART) and a magnesium alloy designed to extend longevity (SMARTER). We deployed 127 longlines with SMART hooks, SMARTER hooks, controls, and procedural controls from 2021-2022 in Winyah Bay, South Carolina, and caught …

Synaptic Properties Of Parabigeminal Circuits., Kyle Whyland

Electronic Theses and Dissertations

Subcortical structures of the visual system have been the subject of intense study in recent years, but there remain some important unanswered questions regarding the synaptic relationships linking the nuclei that comprise this important sensory network within the brain. In these studies, we use several modern and traditional approaches, including viral tract tracing, in vitro slice physiology, immunohistochemistry, optogenetics, and electron microscopy to characterize the circuits linking the superior colliculus (SC), parabigeminal nucleus (PBG), and lateral geniculate nucleus (LGN), with particular focus on GABAergic and cholinergic cell types. We found that the SC, an important visuomotor structure with connections to …

An Evaluation Of Hospital-Based Health Technology Assessment Processes In The United States., Scott Skinner

Electronic Theses and Dissertations

This research study explored the practices of hospital-based health technology assessment (HTA) in the U.S., as experienced by healthcare technology management (HTM) professionals. HTA can play an important role in helping to evaluate health technologies at both the macro (e.g., policy) level as well as at the micro (e.g., institutional) level of the public health ecosystem (C. S. Goodman, 2004). HTA seeks to broadly evaluate the effects, properties, and other impacts of various health technologies (C. S. Goodman, 2004). Common goals of HTA are to evaluate the safety, effectiveness, and cost-benefit of health technologies. A primarily phenomenological approach was used …

“The Urgent Necessity For More Original Creative Works By Transgender And Gender Expansive Artists" Is Not The Title Of My Thesis., Alicia Fireel

Electronic Theses and Dissertations

This thesis is a mix of personal reflection, academic research, and an examination of The Girl Crazy Queer and Other Fairy Tales, a play I wrote and performed in October of 2023, co-produced by The University of Louisville’s Department of Theatre Arts and Pandora Productions, a Louisville-based theatre company dedicated to Queer theatre. This thesis and the play mirror each other; both contemplate Queer experiences, Queer histories, Queer trauma, and Queer stories. In the thesis I accomplish this through examining four subjects: myself, my play, the connection between modes of storytelling and aspects of transgender Queerness, and the perils that …

An Investigation Into Structural Plasticity In Peripheral Taste Neurons Associated With Taste Cell Turnover., Zachary Whiddon

Electronic Theses and Dissertations

The continual replacement of taste cells creates interesting questions regarding how the innervating neurons are impacted during this process. Here we ask how innervation within taste buds is affected when taste cell entry is inhibited and reestablished. Inhibition of sonic hedgehog signaling (Shh) is thought to inhibit taste cell turnover. Consistently, fewer new cells were added to individual taste buds after treatment with a Shh-inhibitor compared to vehicle treatment, and taste bud volume decreased after 16 days of treatment. We next examined how taste nerve fiber extension into the gustatory epithelium is affected by preventing taste cell turnover. Ten days …

The Forest For The Trees: How A Local Arboretum Shapes Rhetoric And Discourse Surrounding Environmentalism., Cooper Day

Electronic Theses and Dissertations

Environmental communication has been situated as a crisis discipline; however, scholars have recently explored how to shift to include care within the discipline. To be sure, this does not mean the crisis element should be abandoned, but, instead coupled with care so that environmental messages are positive and forward looking. This project contributes to this shift by looking at how green spaces are constructed to deliver messages of environmental care. More specifically, I analyze how Bernheim Arboretum and Research Forest, located about 30 miles South of Louisville, KY, has been constructed and works to promote environmental care in a variety …

Tumor Targeting Gold Nanoparticles For Delivery Of Rna And Dna Oligonucleotide Therapies For Glioblastoma., Nicholas Allen

Electronic Theses and Dissertations

Glioblastoma (GBM) brain tumors are highly aggressive gliomas due to genetic and cellular heterogeneity. Current GBM treatment consists of surgical resection of the tumor combined with radio- or chemo-therapies. While these treatments have increased the life expectancy for GBM patients up to 20 months, they have had little effect on the 5-year survival rate. The complex cellular and genetic composition of the tumor makes current treatments less effective long term. One approach to developing more effective GBM treatments is to customize nanoparticle-based drug delivery systems that can directly target the aberrant gene expression patterns within a particular GBM tumor. Delivery …

Profitable, Alternative Income Generation And Improved Quality Of Life Among Global Beekeepers, Chris Honeycutt

Electronic Theses and Dissertations

Interventions that promote alternative income generation (AIG) to address social determinants of health are associated with increased household income and improved health outcomes. Organizations have used beekeeping to address poverty and other development goals with mixed outcomes. Beekeeping presents a viable option to promote AIG. Organizations increase their risk of failing to achieve positive outcomes when beekeeping interventions are inadequate. This project aims to develop an organizational assessment tool that measures organizational position in relation to evidence-based factors for income generation and improved quality of life among global beekeepers. The assessment is organized into seven domains that correspond with PRECEDE-PROCEED …

Cardiovascular Disease In Central Appalachia, An Exploratory Study Of Behavioral, Community, And Patient-Centered Care Influences, Kristy Gagnon

Electronic Theses and Dissertations

The Central Appalachian region of the United States disproportionately experiences higher rates of cardiovascular diseases (CVD) and associated risk factors. Primary risk factors for CVD include hypertension, hypercholesterolemia, and tobacco use, all of which have the potential to be mitigated through lifestyle behavior changes. Dietary and physical activity practices are the two main driving forces for the development of hypertension and hypercholesterolemia, while tobacco use is a behavioral choice on its own. Subsequentially, shifting lifestyle choices to emphasize healthier living has the potential to reduce CVDs within the Central Appalachian region. Considering behavioral choices are not made in isolation and …

Vibroacoustic Response Of The Tympanic Membrane To Hyoid-Borne Sound Generated During Echolocation In Bats, Chelsie Snipes

Electronic Theses and Dissertations

The hyoid apparatus in laryngeally echolocating bats forms a mechanical connection between the larynx and auditory bullae and has been hypothesized to transfer the outgoing echolocation call to the middle ear during echolocation call emission. We used µCT data to build models of the hyoid apparatus and middle ear from six species of bats and used finite element modeling (FEM) to measure the vibroacoustic response of the tympanic membrane due to hyoid-borne sound generated during echolocation. We found that hyoid-borne sound in all six species stimulated the eardrum within a range likely heard by bats. Although there were minor differences …

First Skulls Of Arctomeles Dimolodontus (Mustelidae: Melinae) From The Gray Fossil Site (Early Pliocene, Tn) Reveal Extreme Intraspecific Variation, Charles Bruce

Electronic Theses and Dissertations

Here, a morphological description of the extinct meline badger Arctomeles dimolodontus from the Gray Fossil Site (GFS), Tennessee is provided with comparison to extant Meles meles. Originally described solely on upper teeth of a single individual, the recovery of several crania and jaws provides a sample of at least five individuals, affording the largest known fossil sample in North America of the enigmatic Melinae. Examination of A. dimolodontus skull material reveals extreme intraspecific variation in previously identified diagnostic characters, highlighting the need for caution when identifying fossil taxa based on similarly fragmentary remains. Dental variation is likely a result …

Emerging Tick-Borne Diseases In Northeast Tennessee, Jacob Schultz

Electronic Theses and Dissertations

Tick populations have been immigrating into northeast Tennessee from east Tennessee, Kentucky, Virginia, and North Carolina. Counties in states bordering northeast Tennessee harbor tick species associated with human illness. Human diseases transmitted by ticks include ehrlichiosis, spotted fever rickettsial group diseases, tularemia, anaplasmosis, babesiosis, Lyme disease, alpha-gal syndrome, Heartland virus, Powassan virus, and southern tick-associated rash illness (STARI). These diseases cause morbidity and mortality in human populations and may pose a high risk to individuals, wildlife, and livestock. The Cherokee National Forest covering the east Tennessee border provides a permissible environment for ticks to immigrate and thrive. Residents of northeast …

The Reinforcement Enhancing Effects Of Delta-9-Tetrahyrdrocannabinol (Thc) In Male And Female Rats, Kynah Walston

Electronic Theses and Dissertations

Cannabis is widely consumed by humans for pharmacological effects that are mediated by THC, though there is little evidence that THC is a primary reinforcer in non-human animal models. We hypothesized that THC may have potent reinforcement enhancing effects, comparable to other drugs (e.g., nicotine and caffeine) which are also widely consumed by humans, but difficult to establish as primary reinforcers in non-humans. In three experiments with male and female rats saccharin (SACC) or a visual stimulus (VS) served as reinforcers for operant behavior. We explored several pharmacological parameters of THC on responding for SACC or VS, including THC dose, …

Tell The Bees That Transcendentalism Is Lost: The Search For The Lost Transcendental Space In The Bee Poetry Of Emily Dickinson And Sylvia Plath, Adyson M. Ross

Electronic Theses and Dissertations

Ralph Waldo Emerson’s bee poem, “The Humble-Bee,” expresses the nineteenth-century transcendentalist philosophy of finding wholeness and oneness in nature while Sylvia Plath’s twentieth-century bee poems function as a response to Plath’s feelings of alienation and repression, indicating that transcendental peace is lost in the postmodern era. Emerson’s poem indicates the spiritual fulfilment found through observing bees and highlights the harmony between humans and nature, but women of the nineteenth century find difficulty achieving this same level of freedom; Emily Dickinson reclaims the language of transcendentalism in her bee poetry to explore a world otherwise denied to her. The effects of …

Identifying Taphonomic Distribution Patterns At The Gray Fossil Site, Shawn Haugrud

Electronic Theses and Dissertations

Since the early days of the discovery of the Gray Fossil Site (GFS), meticulous efforts to preserve the spatial data were a priority. Direct surveying of fossils prior to recovery, as well as grid mapping the site, provided relative spatial data within a square meter. Such efforts meant that even fragments and microfossils, recovered during the screening operations and eventual concentrate picking, maintained some spatial data. Available spatial data are used to identify smaller deposits within the greater system, as well as non-random distribution patterns among a number of GFS taxa. Patterns are particularly pronounced in the large-bodied taxa, Teleoceras …

Inhibiting Survival Of Salmonella During Desiccation Through The Use Of Naturally Occurring Signals, Joseph Headrick

Electronic Theses and Dissertations

A rising problem in agriculture is the increase of antibiotic-resistant Salmonella cases associated with chicken eggs, which transmit infection to humans. To counter this, new approaches to combat Salmonella in chickens and desiccated on eggshells are vital in the prevention of human foodborne illness. Disrupting signaling pathways with naturally occurring compounds provides a potential novel avenue for prevention of Salmonella infections, as this would disrupt sensing of these environments and inhibit subsequent optimal gene expression. Starting with signals identified in previous studies, salicylic acid was found to inhibit Salmonella desiccation survival on both eggshells and plastic. To expand upon this, …

Witness, Revival, Testimony, Laura Ann Schroeder

Electronic Theses and Dissertations

The artist, Laura Ann Schroeder, discusses her Master of Fine Arts thesis exhibition, Witness, Revival, Testimony, which was installed at Tipton Gallery in Johnson City, TN from March 2, 2023 through March 31, 2023, with a public reception held on March 24, 2023. The exhibition consisted of a collection of sculptural works and installations that evoke scenes and memories from the artist’s childhood. This body of work deconstructs the traditional family dynamic and the private domestic space through recreations of everyday life. The artworks are primarily made with repurposed consumer textiles and techniques like stitching and quilting that have historically …

Consuming The World: Poetic Appetite, Memory, And Identity In Li-Young Lee’S Food Poems, Claire Liszka

Electronic Theses and Dissertations

Food is a universal human necessity, yet food often serves more than a biological purpose as it informs individual and communal identities, and even facilitates memory. This thesis explores personal memory, the development of identity, and an almost reverential connection to nature in several food poems by Li-Young Lee in Rose (1986) and Behind My Eyes (2008). Born in 1957, Lee has been writing poetry since he was young, studying under Gerald Stern in the late 1970s, and he is known for writing sublime, transcendent yet incredibly accessible and expressive poetry. This thesis gives an overview of food studies and …

Mankind Is Machine: A Monstrous Posthuman Reading Of Philip K. Dick’S Selected Works, Gabriel Davis

Electronic Theses and Dissertations

The works of Philip K. Dick act as an ideal template for readers to explore what it means to be human in a technologically dominated world. Dick’s emphasis on the usage of androids and artificial intelligence as literary monsters allows for a posthuman reading of the traditional literary monster, notably in how their uncanny nature and behavior helps reveal the synthetic tendencies of humanity. In Do Androids Dream of Electric Sheep?, “Imposter,” and “I Hope I Shall Arrive Soon,” each narrative incorporates artificial intelligence and androids acting as others to reveal the machine-like qualities of Dick’s human characters. This …