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Cloning And Expression Of Dazl In Rana Pipiens, Jamie Boorech Jan 2003

Cloning And Expression Of Dazl In Rana Pipiens, Jamie Boorech

Electronic Theses and Dissertations

The DAZ gene on the human Y chromosome has been implicated in male fertility. Humans and other animals possess an autosomal version of this gene, referred to as DAZ-like or Dazl, which functions in gametogenesis and fertility. In order to expand our knowledge of Dazl, I cloned Dazl from the northern leopard frog Rana pipiens. To obtain the clone, I screened a R. pipiens ovary cDNA library with a RpDazl-specific PCR-generated radioactive probe. RpDazl, as determined by sequencing, is 3.5kb in length, with an 843 nucleotide open reading frame (ORF) and a 3' untranslated region (UTR) of 2635 nucleotides. At …


Transformation Of Arsenic Oxyanions In Bacterial And Model Systems, Ganna Polshyna Jan 2003

Transformation Of Arsenic Oxyanions In Bacterial And Model Systems, Ganna Polshyna

Electronic Theses and Dissertations

The HPLC-UV-Vis method with 7.5 mM sulfuric acid as a mobile phase and 195 nm wavelength detection was used for the separation and quantification of As(III) and As(V) in bacterial media used for growing of Clostridium sp. Strain OhILAs bacteria. In this method, the arsenic species were eluted in order As(V) > As(III), and the retention time were not affected by bacteria media matrix. It has been shown that the shape of the curve of increasing of the As(III) concentration is similar to that of bacteria growing. The main disadvantage of the present method is its failure to separate As(III) ions …


Using Pc12 Cells And Rat Cortical Neurons To Evaluate Poly(Caprolactone) And Collagenous Microcarriers For Applications In Nerve Guide Fabrication, Rebecca Waddell Jan 2003

Using Pc12 Cells And Rat Cortical Neurons To Evaluate Poly(Caprolactone) And Collagenous Microcarriers For Applications In Nerve Guide Fabrication, Rebecca Waddell

Electronic Theses and Dissertations

Tissue-engineered nerve guides can provide mechanical support as well as biochemical stimulation for nerve regeneration. PC12 cells and rat cortical neurons were used to test poly(caprolactone) (PCL), collagenous microcarriers (CultiSphers), and a novel composite of the two materials as an initial step in the fabrication of multi-channeled nerve guides. PC12 cells were found to attach, proliferate and differentiate on laminin-coated PCL and on CultiSphers. When the initial concentration of PC12 cells was increased, the percentage of CultiSphers with cells attached increased as well. Changing the cell culture conditions from static to dynamic, however, had the opposite effect on cell attachment. …


Archaic Effigy Beads : A New Look At Some Old Beads, Jessica F. Crawford Jan 2003

Archaic Effigy Beads : A New Look At Some Old Beads, Jessica F. Crawford

Electronic Theses and Dissertations

No abstract provided.


The Well-Being Of Children As Viewed Through Their Conceptions Of Death, Jennifer Kampmann Jan 2003

The Well-Being Of Children As Viewed Through Their Conceptions Of Death, Jennifer Kampmann

Electronic Theses and Dissertations

An explorative study was conducted to try and understand how young children’s emerging death concepts form including, (a) what family demographics and child factors contributed to family well-being, (b) did family well-being influence children’s social competence, and (c) did family well-being and children’s social competence influence children’s death conceptions. Although the subject of death contains many unique characteristics, it is not easily separated from other aspects of life; death is inseparable from the whole human experience (DeSpelder & Strickland, 2002). It was the assumption of this paper that children develop their conceptions of death based on the appreciation they hold …


Kin Recognition In Captive Wild Turkeys (Meleagris Gallopavo) / By Leigh Ben Truong, Leigh Ben Truong Jan 2003

Kin Recognition In Captive Wild Turkeys (Meleagris Gallopavo) / By Leigh Ben Truong, Leigh Ben Truong

Electronic Theses and Dissertations

No abstract provided.


An Attempt At Balance : Staging Guare's The House Of Blue Leaves, Richard J. Albert Jan 2003

An Attempt At Balance : Staging Guare's The House Of Blue Leaves, Richard J. Albert

Electronic Theses and Dissertations

No abstract provided.


Pheromonal Mediation Of Sex Change In The Bridled Goby, Coryphopterus Glaucofraenum, A Protogynously Hermaphroditic Reef Fish, Kristen Erica Marsh Jan 2003

Pheromonal Mediation Of Sex Change In The Bridled Goby, Coryphopterus Glaucofraenum, A Protogynously Hermaphroditic Reef Fish, Kristen Erica Marsh

Electronic Theses and Dissertations

No abstract provided.


The Development Of Criteria For The Selection Of Wetland Restoration Sites In The Yazoo Backwater Area, Mississippi, Angela A. Davis Jan 2003

The Development Of Criteria For The Selection Of Wetland Restoration Sites In The Yazoo Backwater Area, Mississippi, Angela A. Davis

Electronic Theses and Dissertations

No abstract provided.


Quality Control In Microarrays / By Neelima Rungta, Neelima Rungta Jan 2003

Quality Control In Microarrays / By Neelima Rungta, Neelima Rungta

Electronic Theses and Dissertations

No abstract provided.


Victorian Governesses And The Deconstruction Of The Ideal Of Ladyhood / By Sommer M. Sneed, Sommer M. Sneed Jan 2003

Victorian Governesses And The Deconstruction Of The Ideal Of Ladyhood / By Sommer M. Sneed, Sommer M. Sneed

Electronic Theses and Dissertations

No abstract provided.


Phytochemical And Biological Investigation Of Zanthoxylum Monophyllum (Lam) P. Wilson (Rutaceae), Laura C. Johansmann Jan 2003

Phytochemical And Biological Investigation Of Zanthoxylum Monophyllum (Lam) P. Wilson (Rutaceae), Laura C. Johansmann

Electronic Theses and Dissertations

No abstract provided.


The Tie That Binds : Maternal Influence In The Works Of Charles Reade, Rachel Crawford Mckee Jan 2003

The Tie That Binds : Maternal Influence In The Works Of Charles Reade, Rachel Crawford Mckee

Electronic Theses and Dissertations

No abstract provided.


Effects Of Ph And Amendments On Soil, Niranjan K. Kottala Jan 2003

Effects Of Ph And Amendments On Soil, Niranjan K. Kottala

Electronic Theses and Dissertations

No abstract provided.


The Sensitivity Of Glochidia Of Freshwater Mussel Ligumia Subrostrata To Arsenic And Suspended Sediments, Tomoko Kikuchi Jan 2003

The Sensitivity Of Glochidia Of Freshwater Mussel Ligumia Subrostrata To Arsenic And Suspended Sediments, Tomoko Kikuchi

Electronic Theses and Dissertations

No abstract provided.


Effects Of Polycyclic Aromatic Hydrocarbons On Cyp19 And Cyp1b Expression In Teleosts, Monali Master Jan 2003

Effects Of Polycyclic Aromatic Hydrocarbons On Cyp19 And Cyp1b Expression In Teleosts, Monali Master

Electronic Theses and Dissertations

No abstract provided.


A Light Design Process For Twelfth Night, Erin Elizabeth Alberts Jan 2003

A Light Design Process For Twelfth Night, Erin Elizabeth Alberts

Electronic Theses and Dissertations

No abstract provided.


Hemingway, Bullfight, Code, Man, Nathan Henton Jan 2003

Hemingway, Bullfight, Code, Man, Nathan Henton

Electronic Theses and Dissertations

No abstract provided.


Beauty As Currency, Joseph S. Abide Jan 2003

Beauty As Currency, Joseph S. Abide

Electronic Theses and Dissertations

No abstract provided.


Use Of Bioassays To Determine Receptor-Mediated Induction Of Cytochrome P4501a And Estrogenic Activity In Sediment And Natural Product Samples, Srinivas Annavarapu Jan 2003

Use Of Bioassays To Determine Receptor-Mediated Induction Of Cytochrome P4501a And Estrogenic Activity In Sediment And Natural Product Samples, Srinivas Annavarapu

Electronic Theses and Dissertations

No abstract provided.


A Fractal Analysis Of Estrogen, Estrogen-Receptor Interactions And Estrogen Metabolism In Two Related Species Of Catfish, Harshala D. Butala Jan 2003

A Fractal Analysis Of Estrogen, Estrogen-Receptor Interactions And Estrogen Metabolism In Two Related Species Of Catfish, Harshala D. Butala

Electronic Theses and Dissertations

No abstract provided.


An Examination Of Soil Biogeochemical Properties And Seed Bank Dynamics Of Timber Harvested Lands In Lafayette And Calhoun Counties, Mississippi, Lisa Chambers-Strong Jan 2003

An Examination Of Soil Biogeochemical Properties And Seed Bank Dynamics Of Timber Harvested Lands In Lafayette And Calhoun Counties, Mississippi, Lisa Chambers-Strong

Electronic Theses and Dissertations

No abstract provided.


Protein Folding And Misfolding Mechanisms - Pathological Implications, Detection And Modeling, Nigil Satish Jeyashekar Jan 2003

Protein Folding And Misfolding Mechanisms - Pathological Implications, Detection And Modeling, Nigil Satish Jeyashekar

Electronic Theses and Dissertations

No abstract provided.


Statistically Designed Spheronization And Scale-Up Of Ibuprofen Microparticulates Using The Rotor Disk Fluid-Bed Technology: Coating For Prolonged Release And Hard Gelatin Encapsulation Of Microparticulates, Beatrice Chukwumezie Jan 2003

Statistically Designed Spheronization And Scale-Up Of Ibuprofen Microparticulates Using The Rotor Disk Fluid-Bed Technology: Coating For Prolonged Release And Hard Gelatin Encapsulation Of Microparticulates, Beatrice Chukwumezie

Electronic Theses and Dissertations

The aim was to develop uncoated and coated ibuprofen microparticulates in a one-step fluid-bed machine with rotor-disk insert, for immediate and prolonged drug delivery. Feasibilty studies using ibuprofen: Avicel (RC-581; 50:50), sodium lauryl sulfate (1%) as surfactant and water as binder in FLM-15 Vector Flo-coater with 12" stainless-steel and waffle-disk inserts showed that amount of binder, plate type and the presence of surfactant affected most of spheroid characteristics. These variables were used in a 2x2x3 full factorial (replicated) experiment. Blocking was used to study batch-to-batch reproducibility of the process and product variables. Our results confirmed that the binder amount, plate-type …


Feeding Identity: The Critical Hermeneutics Of Contemporary Kleinian Psychoanalytically Oriented Psychotherapy From The Perspective Of A Novice, David M. Greco-Brooks Jan 2003

Feeding Identity: The Critical Hermeneutics Of Contemporary Kleinian Psychoanalytically Oriented Psychotherapy From The Perspective Of A Novice, David M. Greco-Brooks

Electronic Theses and Dissertations

Clinical supervision is required for every graduate student pursuing a clinical track in psychology. Yet empirical research has focused almost exclusively on identifying efficacious supervision techniques in terms of altering "trainee characteristics" rather than investigating in depth the effects of supervision as changes in the characteristics of the trainee's communicative behavior. Moreover, the question of how the specialized communicative behavior practiced by the trainee works to facilitate change for the client/patient has not been addressed by quantitative empirical studies of supervision or psychotherapy process. This study addresses these problems by asking: (1) What characterizes the author's specialized communicative competence after …


Uncoiling The Gut Of Eleutherodactylus Coqui: Characterization Of The Anatomical Development And Proliferation Pattern, Carrie Elizabeth Langer Jan 2003

Uncoiling The Gut Of Eleutherodactylus Coqui: Characterization Of The Anatomical Development And Proliferation Pattern, Carrie Elizabeth Langer

Electronic Theses and Dissertations

Among the frogs there are two forms of development: biphasic and direct. Xenopus laevis, a biphasic developer, develops from an embryo into a tadpole, and then metamorphoses into an adult frog. Eleutherodactylus coqui is a direct developer, meaning that it lacks a tadpole stage. The embryo develops directly into the adult form. Comparing development between these two frogs provides insight on the evolution of these groups. I present an anatomical description of the gut development of E. coqui, and compare my findings to the development of X. laevis. E. coqui does not have a highly coiled gut like that of …


Regulation Of Vitellogenin And Other Serum Proteins By Estrogen And Xenobiotic Estrogens In Salamanders, Lisa Marie Nespoli Jan 2003

Regulation Of Vitellogenin And Other Serum Proteins By Estrogen And Xenobiotic Estrogens In Salamanders, Lisa Marie Nespoli

Electronic Theses and Dissertations

Environmental estrogens pose potential health risks to humans and wildlife by disrupting physiological and developmental processes; therefore, our laboratory has been designing in vivo bioassays on induction of the egg-yolk precursor protein vitellogenin. I have provided information on vitellogenin induction by estrogens in tiger salamanders (Ambystoma tigrinum), spotted salamanders (Ambystoma maculatum), hellbenders (Cryptobranchus alleganiensis), Morelet's crocodiles (Crocodylus moreletii), and developed vitellogenin immunoassays in these species. Salamanders were estrogen-injected; blood was collected at intervals. Estrogen exposed male salamanders of all species, produced substantial quantities of a 200-kDa protein, the approximate size of vertebrate vitellogenins. Sequencing of estrogen-induced proteins showed substantial homology …


Socratic Encounters: Plato's Alcibiades, Andre Archie Jan 2003

Socratic Encounters: Plato's Alcibiades, Andre Archie

Electronic Theses and Dissertations

The aim of this dissertation is to situate our reading of the Platonic dialogue Alcibiades Major among both ancient and modern readings of the dialogue. Since the nineteenth century the issue of authenticity has preoccupied most modern commentators of the dialogue, but from all reasonable evidence, commentators from the ancient world had no such qualms about attributing the authorship of Alcibiades Major to Plato. Our reading of Alcibiades Major is in line with modern commentators who take both the dialogue's dramatic features and educative value seriously, while not ignoring that in some quarters Alcibiades Major's authorship is still in dispute. …


Frederick Douglass And The Gospel Of Zarathustra / By Neil Segars, Neil Segars Jan 2003

Frederick Douglass And The Gospel Of Zarathustra / By Neil Segars, Neil Segars

Electronic Theses and Dissertations

No abstract provided.


American Shame : The Psychodynamics Of Willa Cather's The Song Of The Lark, Stephen Monroe Jan 2003

American Shame : The Psychodynamics Of Willa Cather's The Song Of The Lark, Stephen Monroe

Electronic Theses and Dissertations

No abstract provided.