Digital Commons Network^™

Cell Toxicity And Uptake Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs) By Various Cell Ines In Vitro., Christelle Komguem Kamga

Electronic Theses and Dissertations

This research focused on investigating and comparing the cytotoxicity and cellular uptake of RRR-alpha-tocopheryl polyethylene glycol succinate (TPGS, with that of alpha-tocopheryl succinate (α-TS). Both TPGS and α-TS are water-soluble forms of vitamin E with important clinical applications. Cytotoxicity assays with RAW 264.7 and LNCaP cells incubated overnight with TPGS or α-TS at concentrations ≥ 12.4 μM suggest that α-TS is more cytotoxic than TPGS. Macrophages were found to be more sensitive than LNCaP cells when treated with similar concentrations of α-TS. For both cell lines, most of the TPGS or α-TS taken up remained esterified after 24 hours. Our …

Aggression: Relationships With Sex, Gender Role Identity, And Gender Role Stress., Robin L. Leonard

Electronic Theses and Dissertations

Sex, gender-role identity, and gender-role stress were assessed in terms of their relationship to observed gender differences in self-reported aggression. Physical and verbal aggression were explored, as well as the affective component of anger and cognitive component of hostility. The role of emotional intelligence in these relationships was also evaluated, as a possible correlate to the gender-related variables. The results indicated that both gender-role stress and gender-role identification were significantly associated with all components of aggression; however, only physical aggression was related to sex. Emotional intelligence was linked to sex and gender-role identity but not with gender-role stress. The results …

Trapped In Bluebeard's Chamber: Rose Terry Cooke And Nineteenth-Century "Desperate Housewives.", Bridget Renee Garland

Electronic Theses and Dissertations

Often overlooked in the study of nineteenth-century American literature, the New England writer Rose Terry Cooke elicited great popular appeal during the peak of her career. The admiration Cooke received from her readers and fellow writers compels one to question Cooke’s present-day obscurity. Cooke’s fiction and poetry seem inconsistent with the attitudes she express in her non-fiction, particularly concerning religion and women’s suffrage. She portrays women in miserable marriages, desperately looking for an escape. These “brides of Bluebeard” find different ways to cope with their predicament. While most never truly escape, many use (1) religious devotion, (2) masochism, and (3) …

The Tension Between Art And Science In Historical Writing., Michael Lee Depew

Electronic Theses and Dissertations

A perennial question in the philosophy of history is whether history is a science or an art. This thesis contests that this question constitutes a false dichotomy, limiting the discussion in such a way as to exclude other possibilities of understanding the nature of the historical task.

The speculative philosophies of Augustine, Kant, and Marx; the critical philosophies of Ranke, Comte along with the later positivist, and the historical idealist such as Collingwood will be surveyed. History is then examined along side art to discuss not only the similarities but, the differences.

Major similarities—narrative presentation, emplotation, and the selective nature …

Cost Effective Human Protein C Purification From Cohn Fraction Iv-1 Using Mini-Antibody., Doh Gyeuhn Ahn 1969-

Electronic Theses and Dissertations

Protein C (PC) is an important anticoagulant, antithrombotic, and antiinflammatory in blood plasma. PC deficiency can lead to severe venous thrombotic events, including lung embolism, stroke, and heart attack. In the body, PC is activated only when it is needed, and, therefore, PC does not cause bleeding problems that currently available anticoagulants may do. Purification of PC from plasma is currently done by immunoaffinity chromatography using monoclonal antibodies (Mabs), which is very expensive. Single chain variable regions (mini-Mab) with a PC binding capability, which can be produced in recombinant E. coli , were developed for PC purification. Compared to Mabs, …

Ridge Augmentation Comparing A Cancellous Block Allograft To Freeze Dried Bone Particles And Utilizing An Acellular Dermal Matrix Barrier Membrane., Brenton Lahey

Electronic Theses and Dissertations

Aims. The primary aim of this randomized, controlled, blinded clinical trial was to compare the clinical and histologic results of ridge augmentation comparing a cancellous block allograft to a particulate mineralized freeze dried bone allograft using acellular dermal matrix as a barrier membrane. Methods. Twenty-four patients that met the following inclusion criteria were sequentially entered into the study: 1) at least age 18; 2) had at least a one tooth ridge defect treatment planned to receive a dental implant; 3) the defect was bordered by at least one tooth; and 3) an IRB approved informed consent was signed. Twelve test …

Efficacy Of Cardiac Education On Dietary Habits Of Subjects Participating In Cardiac Rehabilitation., Marie-Rose Antoine Abdo

Electronic Theses and Dissertations

The purpose of this study was to determine if cardiac nutrition education can affect dietary patterns of subjects participating in Cardiac Rehabilitation. Surveys, a pretest given prior to the first cardiac class and a second identical test, administered following cardiac rehabilitation classes, were conducted. Fifty-five randomly selected participants from the outpatient Cardiac Rehabilitation Program were initially given the posttest. Thirty-three of the 55 surveys were returned. Cardiac health classes offered by various health professionals included cardiac nutrition education, group nutrition classes, guest lectures on nutrition, and cooking demonstrations. The tests were compared to measure changes in diet and were analyzed …

A Comparison Of Academic Achievement And Value-Added Grades On The State Report Cards In Tennessee, 2001-2003., Kyle Allen Evans

Electronic Theses and Dissertations

This study uses the state report cards published by the Tennessee Department of Education to compare the academic achievement grades to the value-added grades to determine if there is a relationship between the two grading systems. The data used for this study are from the 2001, 2002, and the 2003 state report cards published for each school using the five subject areas of reading, language, math, science, and social studies.

One thousand sixty schools in the state of Tennessee were for this study. The socioeconomic status (SES) of the schools was used as a covariate to determine if the socioeconomic …

A Comparative Study Of The Perceptions Of Elementary School Administrators, Teachers, And Students Regarding Recess And Free Play In The Public School., Amy Bennett Banner

Electronic Theses and Dissertations

According to recent studies, the number of schools that have severely limited or eliminated recess and free-play opportunities is on the rise across the nation. School officials cite the increasing levels of state and federal pressure to perform on standardized tests as the primary reason for this shift away from the playground. The threat of lawsuits and safety concerns are also listed as factors in this change of policy.

The purpose of this mixed methods study was to examine the perceptions of directors of schools, supervisors, principals, assistant principals, teachers, and students regarding recess and free play in three East …

A Solemn Assembly., John Michael Cline

Electronic Theses and Dissertations

A group of oil paintings completed in partial requirement of my MFA degree is discussed. The paintings are on wood panels and are the result of a combination of old master techniques of under-painting and glazing and more contemporary approaches to the painting process. Each painting represents a particular concept or event from Mormon theology; whereas, the pictorial structure is inspired by Medieval manuscript painting. Thus, this body of work is a synthesis between two worldviews existing centuries apart, yet sharing certain core values and beliefs.

Social Disruption In The Gothic Novels Of Horace Walpole, Elizabeth Inchbald, And Jane Austen., Lia Criselda Lim Pun-Chuen

Electronic Theses and Dissertations

The Gothic novel plays on the exaggeration of prescribed sex roles and uses various narrative techniques to produce a social commentary on gender politics and to illustrate the consequences of a destroyed social structure. Through the examination of the construct of the Gothic narrative and its fragmentary style, the novels of Horace Walpole, Elizabeth Inchbald, and Jane Austen reveal similar treatments of the sexuality of their characters. The implementation of key Gothic elements—such as the castle, tyrannical father, and distressed damsel—serve to propel the novels’ questioning of the patriarchal system, the theme of women as commodities, and the economic value …

Storytelling And Story Reading: A Comparison Of Effects On Children's Memory And Story Comprehension., Matthew P. Gallets

Electronic Theses and Dissertations

For years, storytellers have been going to schools to share stories with children. However, to date only limited research has been done on the effects of storytelling on children’s learning. This project was part of an ongoing study involving several researchers. In this portion of the project, the effects of storytelling and story reading were compared. The population studied consisted of kindergarten, first, and second grade students. Half the students were read stories aloud, the other half were told the same stories by a storyteller. Data were collected regarding students ability to recall facts they had heard, as well as …

Chronic Olanzapine Treatment Eliminates Cognitive Deficits Produced By Neonatal Quinpirole Treatment., Stephanie K. Thacker

Electronic Theses and Dissertations

This study evaluated the effects of chronic olanzapine treatment on cognitive performance and neurochemical function in a rodent model of schizophrenia. Animals were neonatally treated with quinpirole, a dopamine D₂ receptor agonist, or saline. Quinpirole treatment produces an increase of dopamine D₂ receptor sensitivity that extends into adulthood, known as D₂ receptor priming, similar to a phenomenon that occurs in schizophrenia. These same rats were treated in adulthood for 28 days with olanzapine, an atypical antipsychotic, or saline. Dopamine D₂- primed rats demonstrated significant deficits on a cognitive task that were alleviated by olanzapine treatment. …

Graph Theory For The Secondary School Classroom., Dayna Brown Smithers

Electronic Theses and Dissertations

After recognizing the beauty and the utility of Graph Theory in solving a variety of problems, the author decided that it would be a good idea to make the subject available for students earlier in their educational experience. In this thesis, the author developed four units in Graph Theory, namely Vertex Coloring, Minimum Spanning Tree, Domination, and Hamiltonian Paths and Cycles, which are appropriate for high school level.

Primary Sex Reversal In Female Betta Splendens Following Treatments With Temperature And Trenbolone Acetate., Tracy S. Smith

Electronic Theses and Dissertations

Sex reversal in fish can occur as a result of disturbances in natural conditions or laboratory manipulations. Trenbolone Acetate (TBA), a synthetic anabolic-androgenic steroid used mainly in the cattle industry, was applied to female Betta splendens, a sexually dimorphic fish. Previously, females were analyzed for secondary sex characteristics after treatment with TBA, increased temperature, or a combination of both. This study focused on primary sex characteristics by histological examination. Gonads were surgically removed and prepared following basic histological techniques. Following H&E staining, sections were observed for signs of ovarian regression, as well as signs of testicular formation. The presence …

Mud And Ashes., Timothy Mcdonald

Electronic Theses and Dissertations

The artist discusses the work in Mud and Ashes, his Master of Fine Arts exhibition held at Slocumb Galleries, East Tennessee State University, Johnson City, Tennessee, from March 28 to April 1, 2005. The exhibition marks a turning point in the artist’s work, occupying a beginning place as he completes his graduate career. The work consists of fifteen paintings and one sculptural installation. The paintings are on paper and employ local materials such as red clay, pollen, and beeswax along with traditional artist’s materials such as oil and charcoal. Fire and a power sander have also been used as …

Characterization Of Microbial Communities In Karstic Sediments : A Study Of Cave Bacteria In Central Kentucky., Justine Kay Roback 1964-

Electronic Theses and Dissertations

Few previous studies have compared microbial communities in subterranean and surface environments. Chemical analyses used to characterize the surface and cave microbial environments indicated limited exchange between surface and subsurface waters. Here, bacterial communities from four distinct cave sediments collected in central Kentucky and adjacent surface streams were compared using a combination of culture and molecular methods. Predictions were that: 1) Cave communities will have unique community structures when compared to those of nearby surface sediments, and 2) Genetic diversity of bacterial communities in cave sediments will be higher than those in nearby surface sediments. Since diversity and community structure …

Investigations Of N-Acetyltransferases In Human Hepatocytes And Rat Models., Jason Matthew Walraven

Electronic Theses and Dissertations

This thesis begins by reviewing human N-acetyltransferases (Chapter I), then outlines experiments involving human hepatocytes and rat N-acetyltransferases (Chapters II-V). These experiments facilitated the development of a dissertation project designed to study N-acetyltransferase tissue-specific expression in inbred rat strains (Chapter VI). Real-time PCR experiments concluded that genetic and/or environmental factors influence N-acetyltransferase expression in cryopreserved human hepatocytes. Therefore, an animal model must be used to study N-acetyltransferase expression. Initial studies of recombinanatly expressed rat N-acetyltransferases (Nats) revealed a correlation between rat and human N-acetyltransferases in nucleotide and amino acide sequence, catalytic activity, substrate selectivity and thermostability, suggesting the rat is …

Role Of Inducible Nitric Oxide Synthase And P-Selectin In Platelet-Arteriolar Wall Adhesion And Associated Arteriolar Constriction During Lung Reperfusion., Alexander V. Ovechkin 1957-

Electronic Theses and Dissertations

Reperfusion of the ischemic lung causes pulmonary arteriolar vasoconstriction and reduces alveolar perfusion. Lung ischemia-reperfusion (IR) injury leads to platelet and leukocyte activation which could contribute to decreased alveolar perfusion during reperfusion by platelet-arteriolar wall interactions (rolling and adhesion) and subsequent microvascular constriction. During the oxidative stress of ischemia and reperfusion, formation of reactive nitrogen species (RNS) may promote platelet adhesion to the vascular wall with subsequent formation of microthrombi and release of vasoactive substances. Platelets play an important role in causing reperfusion injury in the systemic vasculature through accumulation and release of mediators, but information is lacking about consequences …

General Ishii Shiro: His Legacy Is That Of Genius And Madman, Gregory Dean Byrd

Electronic Theses and Dissertations

This paper covers the development of the chemical weapons division founded by Ishii Shiro, and discusses the horrible experimentation that was done by the Japanese. These experiments have been a source of controversy. The Chinese feel the Japanese should acknowledge these as war crimes. When the Japanese left Manchuria, they left the world’s largest chemical waste dump behind, and even to this day the government refuses to admit the actions of Unit 731. The information on biological warfare that the Japanese discovered during the experimentation in China later was used as a negotiation tool with the United States to secure …

Effects Of Food Deprivation On Blood Lipid Concentration And Composition In Steller Sea Lions (Eumetopias Jubatus), Michelle Lea Berman

Electronic Theses and Dissertations

Steller sea lions, the largest Otariid, fast during their breeding season; during this time they refrain from ingesting food for a period of 12-43 days. Fasting, while undertaking an extremely energetically demanding activity (breeding and pupping), requires specific physiological adaptations. This study examined the physiological response to fasting of two age classes, juveniles and sub-adults, during the breeding and non-breeding seasons to determine how these animals utilize lipids and the pattern of fatty acid mobilization from lipid stores during fasting. Four juveniles and 5 sub-adults were fasted for one and two weeks, respectively, and blood samples were collected approximately every …

Communicating Culture Through Capoeira, Desislava Georgieva

Electronic Theses and Dissertations

The popularity of capoeira, a dance based in the martial arts and originating among slaves in Sixteenth-Century Brazil, has increased greatly in recent decades as it has spread worldwide as a performance representative of Brazilian history and culture. In 1974, capoeira was declared the national sport of Brazil. Today capoeira academies and competitions may be found wherever Brazilian culture is celebrated and communicated in many major foreign cities. My thesis, through the participant/observer method and the use of long interviews, examines capoeira as it is practiced in one particular academy far from the art form's place of origin. In the …

Novel Protein-Protein Interactions Regulate The Proteolytic Activity Of The Pro- Apoptotic Serine Protease, Omi/Htra2, Supriya Singh

Electronic Theses and Dissertations

Omi/HtrA2 is a mitochondrial serine protease with high homology to the bacterial HtrA proteins. Omi promotes caspase-dependent apoptosis by binding and degrading IAPs-inhibitor of apoptosis proteins. Omi can also induce caspase-independent apoptosis but the actual mechanism is still unknown. IAP's are not the only substrates cleaved by Omi. There are at least two more known substrates of Omi, the HAX-1 and the ped/pea-15 proteins. HS1-associated protein X-1 (HAX-1) is a mitochondrial protein, degraded by Omi after induction of caspase-dependent apoptosis. Ped/pea-15 is also an anti-apoptotic protein and is cleaved by Omi after induction of caspase-independent apoptosis. The proteolytic activity of …

Responses Of The Florida Mouse (Podomys Floridanus) To Habitat Management, Jason Depue

Electronic Theses and Dissertations

The Florida mouse (Podomys floridanus), a species restricted to the Lake Wales Ridge and the Atlantic Coastal Ridge, is recognized by the Florida Fish and Wildlife Conservation Commission as a species of special concern, highlighting its status as a species that is threatened by habitat loss statewide. Publicly owned lands offer protection for the species, but management is generally focused on protecting biodiversity in general and not a particular species. The response of the Florida mouse to land management practices such as mechanical treatment and prescribed fires is poorly documented. This research examined the population responses of Florida mice on …

Real Time Rt-Pcr For Direct Detection Of Viable Mycobacterium Avium Subspecies Paratuberculosis In Chron's Disease Patients And Association Of Map Infection With Downregulation In Interferon-Gamma Receptor (Infg1) Gene In Crohn's Disease Patients, Mounir Chehtane

Electronic Theses and Dissertations

Association of Mycobacterium avium subspecies paratuberculosis (MAP) with Crohn's disease (CD) and not with ulcerative colitis (UC), two forms of inflammatory bowel disease (IBD), has been vigorously debated in recent years. This theory has been strengthened by recent culture of MAP from breast milk, intestinal tissue and Blood from patients with active Crohn's disease. Culture of MAP from clinical samples remained challenging due to the fastidious nature of MAP including its lack of cell wall in infected patients. The advent of real time PCR has proven to be significant in infectious disease diagnostics. In this study, real time reverse transcriptase …

Expression Of Hepatitis C Viral Non-Structural 3 Antigen In Transgenic Chloroplasts, Anubhuti Bhati

Electronic Theses and Dissertations

Hepatitis C viral infection is the major cause of acute hepatitis and chronic liver disease and remains the leading cause of liver transplants (NIH). An estimated 180 million people are infected globally (WHO). There is no vaccine available to prevent hepatitis C. The treatment with antiviral drugs is expensive, accompanied with various side effects and is limited only to those at risk of developing advanced liver disease. The treatment is also effective in only about 30% to 50% of treated patients and still a high percentage of patients are resistant to therapy. Therefore, there is an urgent need for the …

The Effects Of Ecotourism On Polar Bear Behavior, Gillian Eckhardt

Electronic Theses and Dissertations

Polar bears spend the majority of their lives on the sea ice, where they gain access to seals and mates. In western Hudson Bay, the sea ice melts for three to four months in the summer, and polar bears there are forced onto land. These bears live on their fat reserves for the duration of the iceless period, until temperatures get colder in the fall and freeze up begins. The aggregation of polar bears near Churchill, Manitoba during the ice free period has led to a thriving tourist industry, with a large influx of tourists visiting Churchill in the fall …

Expression Of Cholera Toxin B Subunit-Rotavirus Nsp4 Enterotoxin Fusion Protein In Transgenic Chloroplasts, Anila Kalluri

Electronic Theses and Dissertations

Rotavirus, the major cause of life-threatening infantile gastroenteritis, is a member of the Reoviridae family and is considered to be the single most important cause of virus-based severe diarrheal illness in infants and young children particularly 6 months to 2 years of age in industrialized and developing countries. Infection in infants and young children is often accompanied by severe life threatening diarrhea, most commonly following primary infection. Diarrhea is the major cause of death among children around the world. Responsible for 4 to 6 million deaths per year according to the World Health Organization (WHO), diarrhea is especially dangerous for …

A Theoretical Approach To Assessing Annual Energy Balance In Gray Whales (Eschrichtius Robustus), Nathalie Lucie Elizabeth Greenwald

Electronic Theses and Dissertations

While direct measurements of energetic demands are nearly impossible to collect on large cetaceans, comprehensive bioenergetic models can give insights on such parameters by combining physiological and ecological knowledge. This model was developed to estimate necessary food intake of gray whales, Eschrichtius robustus, of the Eastern North Pacific stock. Field Metabolic Rates (FMR) for gray whales were first estimated based on various assumptions (e.g. volumetric representation of gray whales, extent of their feeding season, and blubber depth distribution) using morphometric data, energetic costs, and food assimilation according to age and gender specific requirements. Food intake rates for gray whales of …

Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts, Arati Limaye

Electronic Theses and Dissertations

The skyrocketing costs of prescription medicine in developed countries and their lack of availability in developing countries are the most challenging problems of human health. Primary reasons for such high cost are fermentation-based production, expensive purification methods, the need for low temperature storage and transportation and the delivery through sterile injections. Most of these expenses could be minimized or eliminated when therapeutic proteins are expressed and orally delivered via plant cells. Chloroplasts have the machinery to fold complex and biologically active eukaryotic proteins in the soluble chloroplast stromal compartment. Protein expression through chloroplast transformation system offers a number of advantages …