Digital Commons Network^™

Immunomodulation Of Myeloid-Derived Suppressor Cells By Particulate B-Glucan In Cancer., Sabrin Husein Albeituni

Electronic Theses and Dissertations

Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of immature myeloid cells that promote tumor progression. In this study, we investigated the effect of dectin-1 stimulation by yeast-derived particulate β-glucan in MDSC function and differentiation in cancer. In vivo treatment of mice bearing lewis lung carcinoma and mammary cell carcinoma with particulate β-glucan decreased tumor weight and splenomegaly, and reduced the accumulation of polymorphonuclear-MDSC (PMN-MDSC) but not monocytic-MDSC (M-MDSC) in the spleen and tumor. In addition, particulate β-glucan differentially modulated the function of different MDSC subsets; it enhanced PMN-MDSC respiratory burst and apoptosis, and induced the differentiation of M-MDSC into …

A Culturally-Sensitive Model Of The Development Of Child Anxiety., Jenny Marie Petrie

Electronic Theses and Dissertations

Explanatory models significantly enhance the understanding of etiological influences that place children at risk for anxiety, yet little is known about processes that promote resilient outcomes in children. While contextual factors influence risk and protective processes, cultural constructs have not been incorporated into existing models of anxiety and the role of culture remains obscure. The current study proposes a culturally sensitive model for understanding the etiological and mitigating processes underlying anxious symptoms in ethnic minority youth, and preliminarily tests basic components of the proposed model within a non-clinical community sample of 49 African American (AA) parent-child dyads who completed self-report …

The Impact Of An Interactive Technology Application On Elementary Student Achievement In Fractions., Shannon M. Stone

Electronic Theses and Dissertations

Data reported locally and nationally show that students struggle to understand fractional concepts. The purpose of this study was to examine the effects of an interactive tablet application on student achievement in fractions. Students in grades three through five at the treatment school worked with an interactive tablet application designed to facilitate student construction of fractions knowledge. The interactive tablet application is an open-platform that allowed teachers the autonomy to work with the researcher to load their desired content activities on the topic of fractions to develop instructional sessions for students. Students worked independently during the math workshop to complete …

Mechanisms And Intervention Strategies For Alcohol And Hiv-Antiretroviral Therapy-Induced Liver Injury., Hridgandh Donde

Electronic Theses and Dissertations

Chronic alcohol consumption is a leading cause of liver disease and liver-related death worldwide. Alcoholic liver disease includes, hepatic steatosis, steatohepatitis and ultimately fibrosis and cirrhosis. Emerging evidence has established the important role of the “gut-liver” axis in the development of alcoholic liver disease (ALD). Our recent work indicated that chronic alcohol induced perturbations in the gut microbiome and consequent changes in fatty acids have a major impact on the development of intestinal barrier dysfunction and ALD. The aim of this study was to investigate whether treatment with tributyrin - a butyrate prodrug results in protection against ALD in terms …

Places In The Polity Of Rhetoric : Topoi, Evolution, And The Fragmentation Of Discourse., Benjamin James Bickel Wetherbee

Electronic Theses and Dissertations

This dissertation integrates classical rhetoric with postmodern understandings of textual fragmentation. “Places in the Polity of Rhetoric” follows two mutually constitutive avenues of inquiry, one of which stresses the importance of understanding textual fragments as rhetorical topoi—that is, as generative “places” that allow writers and speakers to economically evoke larger fields of cultural meaning in the space of a single word, phrase, or image. The other stresses the evolution of rhetorical culture that emerges through the interaction between human agents, who use these topoi for rhetorical ends, and discursive agents (topoi themselves) who use human rhetors to propagate …

Dietary Carotenoids And The Complex Role Of Redness In The Behavior Of The Firemouth Cichlid Thorichthys Meeki., Sarah Anne Fauque

Electronic Theses and Dissertations

This dissertation takes a comprehensive approach to the role of dietary carotenoids on redness and the subsequent behaviors in the firemouth cichlid, Thorichthys meeki. I start with a brief introduction into signaling, the importance of carotenoids, and mate choice. The dissertation is then divided into three data chapters which are designed to stand as independent manuscripts. Chapter II documents how altering the availability of dietary carotenoids affects redness in the integument of male and female T. meeki. I tracked how redness changed in color and distribution in individuals over the course of 12 weeks. I confirm that a dichotomy …

Genomic And Transcriptomic Analyses Of Microbotryum Lychnidis-Dioicae Provide Insights Into The Biology Of A Fascinating Fungal Phytopathogen., Su San Toh

Electronic Theses and Dissertations

This study made use of the Silene latifolia/Microbotryum lychnidis-dioicae phytopathogen system as the focal system to establish the first reference genome for Microbotryum violaceum sensu lato. In silico analysis was performed on the genome assembly to identify various characteristics of the genome. Using RNA-Sequencing technologies on the Illumina platform, we collected transcriptomic data for both in vitro and in planta life stages of the fungus, providing the most comprehensive look at the gene expression and regulation of this fungus. Due to a lack of identifiable domains on the predicted genes, gene set enrichment analysis was done in …

Effects Of A Software Program Vs. Constant Time Delay In The Acquisition Of Sight Words For A Student With Significant Disabilities, Turkie Ali Algharbie

Electronic Theses and Dissertations

The development of sight words is essential for individuals with significant disability. There is a plethora of research highlighting the acquisition of sight words for this population but to date, most focus on teacher led interventions (Browder, Algrim-Delzell, Spooner, Mims, & Baker, 2009). This study investigated the use of computer technology vs. one-on-one instruction targeting sight words acquisition for a student with a significant disability. Results showed the participant indicated improved performance using the computer based intervention versus the constant time delay instructor led intervention.

Functional Characterization Of Aggregatibacter Actinomycetemcomitans Qsebc : A Bacterial Adrengeric Receptor And Global Regulator Of Virulence., Whitney Weigel

Electronic Theses and Dissertations

In order for a pathogen to successfully colonize the host, it must be able to acquire essential nutrients and regulate gene expression to respond to environmental fluctuations. One mechanism bacteria have evolved in order to detect these fluctuations and respond is the two component signal transduction systems (TCS). Aggregatibacter actinomycetemcomitans, a dental commensal associated with localized aggressive periodontitis, contains a TCS designated QseBC that was previously shown to regulate expression of several iron acquisition genes, biofilm formation and virulence, but the activating signal was unknown []. QseBC is a conserved TCS in the Enterobacteriaceae and Pasteurellaceae, but the activating signal …

Perceptions Of Acceptable Behavior With Social Networking Among Teachers, Parents, And Students In A Northeast Tennessee Middle School: An Exploratory Study, Lisa L. Raper

Electronic Theses and Dissertations

The purpose of this study was to investigate the perception of acceptable use of social media among teachers, students, and parents. In doing so the researcher touched on generational factors, communication, instructional practice with social networking and the perceptions of what was acceptable among these 3 groups within a middle school setting.

This qualitative study examined how students, parents, and teachers perceived the use of social networking. 30 subjects were randomly chosen and interviewed: 10 students, 10 parents and 10 teachers. There were 4 emerging themes: (a) types of social networking preferred and the reason, (b) relationships and acceptable usage, …

Influence Of Lactobacillus Rhamnosus Isolated From “Amabere Amaruranu” Cultured Milk On Adipogenesis, Justin E. Kotala

Electronic Theses and Dissertations

This study was performed to test the in vitro effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu”, a traditional Kenyan cultured milk, on 3T3-L1 and Caco-2 cell lines. Cultures of fully mature 3T3-L1 adipocytes were treated with bacterial isolate cell extract (CE), filtered spent broth (FSB) from overnight bacterial culture, or with a PBS control. Expression levels of PPAR³1 and 2, C/EBP±, and ATGL proteins in 3T3-L1 cells were upregulated by FSB treatment. CE treatment did not affect protein expression levels. Expression of MTTP and SREBP-1c proteins in Caco-2 cells showed no change with either treatment. Optical density measurements …

The Lawrentian Woman: Monsters In The Margins Of 20th-Century British Literature, Dusty A. Brice

Electronic Theses and Dissertations

Despite his own conservative values, D.H. Lawrence writes sexually liberated female characters. The most subversive female characters in Lawrence’s oeuvre are the Brangwens of The Rainbow. The Brangwens are prototypical models of a form of femininity that connects women to Nature while distancing them from society; his women are cast as monsters, but are strengthened from their link with Nature. They represent what I am calling the Lawrentian-Woman.

The Lawrentian-Woman has proven influential for contemporary British authors. I examine the Lawrentian-Woman’s adoption by later writers and her evolution from modernist frame to postmodern appropriation. First, I look at the …

Characterization Of Sbip68: A Putative Tobacco Glucosyltransferase Protein And Its Role In Plant Defense Mechanisms, Abdulkareem O. Odesina

Electronic Theses and Dissertations

Plant secondary metabolites are essential for normal growth and development in plants ultimately affecting crop yield. They play roles ranging from appearance of the plants to defending against pathogen attack and herbivory. They have been used by humans for medicinal and recreational purposes amongst others. Glycosyltransferases catalyze the transfer of sugars from donor substrates to acceptors. Glucosyltransferases are a specific type of glycosyltransferases known to transfer glucose molecules from a glucose donor to a glucose acceptor (aglycone) producing the corresponding glucose secondary metabolite or glycone, in this case glucosides. It was hypothesized that SBIP68, a tobacco putative glucosyltransferase-like protein glucosylated …

The Effects Of Alpha Stimulation On Induced Anxiety, Nolan Thomas Hill

Electronic Theses and Dissertations

Anxiety is defined as an emotional and physical reaction that prepares us to confront a feared stimulus. Among the many measureable changes induced by anxiety are changes in facial electromyography (EMG), heart rate (HR), and sweat gland activity (EDG). At a pathological level anxiety interferes with cognitive processes. Currently, when anxiety crosses into the pathological level, it is treated with a variety of therapies that share in their use of periods of exposure to anxiety-inducing stimuli. Several devices have been developed to alter brain activity by transcranial electrical stimulation (TCES). One such device, Alpha-Stim^®, has been shown to …

Against Celsus: Piety In Context, Dustin Janssen

Electronic Theses and Dissertations

This thesis explores Celsus’s and Origen’s differing understandings of what it means to be “pious” (ὅσιος). Celsus conceived of tradition as the norm for determining piety. On the other hand, Origen maintained that the true norm was found in the Logos and Wisdom of God—i.e., Jesus. This dichotomy of understanding is consistent with the backdrop of the religious revolution happening in the Roman world during the early centuries CE proposed by scholars like Guy Stroumsa.

While this thesis does not aim to prove or fully expound on the religious revolution, it will use the shift in religious thought as a …

Youth Graffiti Vandalism : Liminal Perspectives In The Light Of Masculinity, Social Contract Theory And Transformative Process., Alton C. Frabetti

Electronic Theses and Dissertations

American adolescents experience liminality as their rights, obligations and cognitive development place them in a transitional life-period between childhood and adulthood. This liminal period inspires compensatory activities, such as identification with mainstream popular styles, radical adherence to an ideology or religion, or, for those especially struggling, destructive behavior. Graffiti is one such compensatory activity. Its practitioners' risky conduct, as well as their overall claims to exalted artistic activity, are especially appealing to certain males attempting to construct a retrogressive notion of masculinity and self-esteem in response to the further condition of male liminal identity. The practice of graffiti confronts the …

Improved Oncolytic Virotherapy By Increasing Virus Spread Within Tumors., Stephen Wechman

Electronic Theses and Dissertations

Oncolytic adenoviruses (Ads) have great therapeutic potential for lung cancer. Cancer selective E1b-deleted Ads are safe, however their efficacy remains limited clinically. To improve E1b-deleted Ads, Adhz60 was selected for greater anti-cancer efficacy by a process called bioselection, producing AdUV. AdUV preferentially lysed A549 and H1299 lung cancer cells more effectively than both the cancer selective Adhz60 and non-selective Ad5. AdUV induced greater LC3-II expression, relative to LC3-I, indicating that AdUV (30.9-fold) induced autophagy more effectively than Ad5 (12.2-fold) and Adhz60 (7.8-fold) in A549 cells. Mice treated with AdUV had significantly smaller tumors (p-value < 0.001) and prolonged survival (p-value = 0.0005) than mice treated with the negative control AdGFP. These results suggest that AdUV efficiently lyses lung cancer cells in vitro and in vivo.

Chronic Ethanol Exposure Sensitizes The Lung In A Mouse Model Of Endotoxemia-Induced Acute Lung Injury : Potential Role Of Plasminogen Activator Inhibitor-1., Lauren G. Poole

Electronic Theses and Dissertations

The goal of this project is to characterize a new mouse model of alcohol-enhanced acute lung injury (ALI) and to determine the role of plasminogen activator inhibitor-1 (PAI-1) in this model. Male mice (WT and PAI-1-/-) were exposed to ethanol-containing Lieber-DeCarli diet or pair-fed control diet for 6 weeks; some animals were administered intraperitoneal lipopolysaccharide (LPS) prior to sacrifice. Chronic alcohol feeding enhanced induction of the chemokines MIP-2 and KC (murine IL-8 homologues) after LPS injection in wild type animals. This enhanced chemokine expression did not correlate with enhanced pulmonary neutrophil infiltration, however animals exposed to chronic ethanol showed sustained …

Regulation Of Receptor Tyrosine Kinases By Ubiquilin1., Zimple Kurlawala

Electronic Theses and Dissertations

Receptor Tyrosine Kinases (RTK’s) like EGFR, Insulin Receptor, IGF1R and others mediate growth, proliferation and balance between apoptosis and survival. Dysregulation of RTK activity has been identified in a wide range of cancers and contributes to initiation, progression and metastasis of tumors. Ubiquilins (UBQLN) are fairly novel proteins and are studied mostly in the field of neurodegenerative disorders. Our laboratory recently discovered a link between UBQLN1 function and tumorigenesis. UBQLN1 is lost in about 50% of non-small cell lung cancer (NSCLC) cases. We have identified an interaction between UBQLN1 and IGF1R. Furthermore, UBQLN1 regulates activity of IGF1R and EGFR. Following …

Identifying Hepatocellular Carcinoma (Hcc) Cells With Cancer Stem Cell-Like Properties., Harshulkumar M. Pandit

Electronic Theses and Dissertations

Hepatocellular Carcinoma (HCC) is a challenging condition to treat in the clinic due to its distinct tumor phenotype, aggressive growth, and chemotherapy resistance. Cancer Stem Cells (CSCs) are a poorly differentiated subpopulation of cells within the tumor microenvironment, and role of CSCs in tumor initiation and chemotherapy resistance is not well understood in HCC. In this study, we have successfully identified HCC cells with CSC-like properties that showed resistance to Doxorubicin. Using the established serum free culture techniques, we have enriched CSC-like cells among hepatocellular cancer cells. These enriched cells exhibit all CSC-like properties i.e. self-renewal capability, anchorage independent growth …

The Effect Of Protandim Supplementation On Oxidative Damage And Athletic Performance., Seteena Leigh Ueberschlag

Electronic Theses and Dissertations

A double-blind placebo-controlled trial determined the long term effects of Protandim® supplementation on 5-km running performance, as well as on acute and long-term oxidative damage assessed by serum lipid peroxides (Thiobarbituric acid-reacting substances: TBARS). Thirty-eight subjects were placed into one of two groups: an experimental (675 mg/day Protandim®) or control group (675 mg/day corn starch). 5-km runs were performed at baseline and 88 (SD 4) days post-supplementation with samples taken immediately pre- and 10-min post 5-km run, as well as at rest ∼30 and ∼60 days during the supplementation period. There was ~25% week-to-week coefficient of variation of TBARS at …

Suramin Protects From Cisplatin-Induced Acute Kidney Injury., Tess Dupre

Electronic Theses and Dissertations

In rodent models, suramin improves recovery following acute kidney injury (AKI). We hypothesized that suramin would be useful in protection against cisplatin-induced AKI. This hypothesis was tested by pre-treating C57BL/6j mice with suramin prior to cisplatin. Our data indicates that suramin protects the kidney from injury by decreasing cisplatininduced decreases in kidney function. Renal histology indicated that suramin significantly protected from cisplatin-induced AKI. Data indicate that suramin pretreatment attenuated mRNA expression of pro-inflammatory cytokines and chemokines following cisplatin treatment, while also decreasing markers of cell stress and cell death. We utilized the same experimental design with 10-month old FVB mice …

The Regulation And Mechanisms Of Egfr-Mediated Apoptosis In Mda-Mb-468 Cells., Nicole Marion Jackson

Electronic Theses and Dissertations

Background: The Epidermal Growth Factor Receptor (EGFR) is a 170- kilodalton transmembrane protein that belongs to the ErbB family of receptor tyrosine kinases. Upon ligand-mediated activation, the EGFR responsible for cell growth, proliferation, and tissue homeostasis in epithelial cells; however, the EGFR is overexpressed in many human malignancies including MDA-MB-468 cells, a metastatic breast epithelial cell line. Previous studies have indicated that within the MDA-MB-468 cell line, receptors at the cell surface promote cell growth when activated with Epidermal Growth Factor (EGF) ligand. Activated receptors that are internalized to the endosomes however induce apoptosis. This contrasting response at different cellular …

Exercise Mitigates The Effects Of Hyperhomocysteinemia On Adverse Skeletal Muscle Remodeling., Lee Winchester

Electronic Theses and Dissertations

Background: Hyperhomocysteinemia (HHcy) is heavily implicated in diabetes and cardiovascular disease. HHcy is known for causing inflammation and vascular remodeling, particularly through production of reactive oxygen species (ROS) and matrix metalloproteinase-9 (MMP-9). Although its impact on skeletal muscle is rather unclear, HHcy is known to cause skeletal muscle weakness and functional impairment. The impact of HHcy on inflammation makes it seem likely that HHcy causes skeletal muscle fibrosis through induction of inflammatory factors and destructive macrophages. Exercise has been shown to reduce homocysteine levels and therefore, could serve as a promising intervention for HHcy. The purpose of this study was …

Performing A Steady-State Seepage Analysis Using Seep/W : A Primer For Engineering Students., Matthew R. Broaddus

Electronic Theses and Dissertations

This thesis is comprised of five independent modules that were developed to provide a primer to understanding groundwater seepage. These modules serve as the template to conduct steady-state seepage analyses through embankments utilizing SEEP/W, a finite element seepage modeling program. Module 1 and 2 are more theoretical in nature to provide the background knowledge necessary to conduct a steady-state seepage analysis, while Modules 3, 4, and 5 focus more on the application of seepage models and how they can be used to solve various groundwater seepage problems. Supplementary material, including PowerPoint presentations, handouts, and a manual on how to utilize …

Combination Treatment With Conjugated Linoleic Acid And Nitrite Protects Against Myocaridal Infarction., Natia Qipshidze Kelm

Electronic Theses and Dissertations

According to the CDC, the most common type of heart disease is coronary artery disease, which frequently leads to myocardial infarction (MI). Therapeutic approaches to lessen the resulting cardiovascular injury associated with MI are limited. Recently, the management paradigm for cardiac injury has entered the molecular era and microRNAs (miRNAs) have been shown to act as negative regulators of gene expression by inhibiting mRNA translation and/or stimulating mRNA degradation. A single miRNA can modulate physiological or disease phenotypes by regulating whole functional systems. Importantly, miRNAs can regulate cardiac function, thereby modulating heart muscle contraction, heart growth and morphogenesis. MicroRNA-499 (miRNA-499) …

Relational Processes In Prayer : A Reflection Of The Effects Of Culture On Religious Experience., Benjamin David Jeppsen

Electronic Theses and Dissertations

This study explores the mediating effect of one’s perceived relationship with God on the relationship between prayer and mental health in a cultural context. As an interpersonal activity, prayer may operate along relational constructs. Research supports a theoretical mechanism of prayer that aligns with the benefits of supportive interpersonal relationships. It is possible, then, that as relational processes change across dimensions of individualism and collectivism, prayer may mirror these same changes. A model for collectivistic and individualistic approaches to prayer is proposed and placed within a relational model for prayer’s effects on mental health. However, due to instrument failure, the …

Promoting Differentiation And Survival Of Human C-Kit+ Cardiac Progenitor Cells Ex Vivo., Tareq Al-Maqtari

Electronic Theses and Dissertations

c-kit+ cardiac progenitor cells (CPCs) have recently gained much attention due to the therapeutic effects they exert on cardiac function following their administration into the infarcted heart as evidenced by animal studies and by a recent clinical trial (SCIPIO). However, injecting these cells in the heart is associated with poor differentiation into specialized cardiac cell types and with rapid death of the engrafted cells. With the ultimate goal of advancing cardiac stem cell therapy, we sought to facilitate the differentiation of human CPCs into cardiac cell types (e.g. cardiomyocytes, smooth muscle cells, endothelial cells and cardiac fibroblasts) by overexpressing selected …

What We See Changes How We See : Analyzing The Plasticity Of The Horizontal Effect., April Marie Schweinhart

Electronic Theses and Dissertations

The relationship between the processing of orientations by the human visual system has been related to the orientation content of the natural environment; horizontal orientations, while predominant in natural environments, are perceived less well than vertical and oblique orientations are perceived best, though they are least prevalent in the natural world. This ‘horizontal effect’ has further extended the well-studied relationship between visual encoding and natural scene statistics as the differential perception of orientations in broadband scenes inversely matches their differential representation in the natural environment. However, the original hypothesis that this relationship may have evolved across millennia in order to …

Mediation In Literacy : Language, Technology, And Modality., Hem Sharma Paudel

Electronic Theses and Dissertations

The issue of difference in writing, both in terms of language diversity and modalities, has received increasing attention in the context of new developments in technologies, increasing global migration, and intensified intersections of cultural and linguistic practices accompanying these changes. Theories of language and modality are trying separately to develop ways to best respond to the challenges and opportunities brought about by these changes. Responding to scholars’ recent calls for bridging the gap between studies of multilingualism and those of multimodality, this dissertation offers an approach that, instead of separating the study of modality and languages, questions such a tendency …