Digital Commons Network^™

Approaches To Arthropod Conservation : Landscape Genetics, Community Assessment, And Prediction Of Extinction Risk., Victoria Annette Prescott

Electronic Theses and Dissertations

Although urbanization is a leading cause of species extinction throughout the world, the impact of urban development on arthropods is little studied and, as a result, poorly understood. I used three distinct approaches to studying arthropod conservation in North America. First, I used landscape genetics techniques to study the impact of urbanization on gene flow among populations of Rabidosa rabida, the rabid wolf spider. While gene flow was not detrimentally reduced, urban development correlated with a reduction in migration rates among populations, and to my knowledge, this is the first study to document isolation by resistance in spiders. Next, …

Cost Of Care For Hospitalized Patients With Pulmonary Mycobacterial Diseases In The United States., Mary Beth Wells Allen

Electronic Theses and Dissertations

Background: Pulmonary mycobacterial diseases describe both tuberculosis (TB) and nontuberculous mycobacteria (NTM). Few data are available measuring the cost burden of mycobacterial diseases on the national level. The purpose of this study was to evaluate the cost burden and measure emerging trends in hospitalization of pulmonary TB and NTM in the US from 2001 through 2012. Methods: This study was a retrospective community based cost analysis of hospitalized patients with a principal diagnosis of pulmonary mycobacterial diseases from 2001 through 2012. Data for pulmonary TB and NTM were retrieved from the Healthcare Cost and Utilization Project (HCUP), US Department of …

Identification Of Residual Descending Pathways After Human Spinal Cord Injury., Darryn A. Atkinson

Electronic Theses and Dissertations

Spinal Cord Injury (SCI) in humans is a heterogeneous diagnosis, resulting in variable paralysis and paresthesia based on the mechanism, rostro-caudal location, and severity of injury. Both neurophysiological and anatomical studies have suggested that subclinical residual supraspinal-spinal connectivity exists in a subset of individuals deemed to have motor and sensory complete injuries. Recent reports of volitional movement in chronic, motor complete individuals during epidural spinal stimulation have provided compelling evidence that these residual projections may be capable of mediating volitional movement when the functional state of spinal circuitry is electrically modulated. It was the goal of this project to identify …

Spreading Speeds Along Shifting Resource Gradients In Reaction-Diffusion Models And Lattice Differential Equations., Jin Shang

Electronic Theses and Dissertations

A reaction-diffusion model and a lattice differential equation are introduced to describe the persistence and spread of a species along a shifting habitat gradient. The species is assumed to grow everywhere in space and its growth rate is assumed to be monotone and positive along the habitat region. We show that the persistence and spreading dynamics of a species are dependent on the speed of the shifting edge of the favorable habitat, c, as well as c^*(∞) and c^*(−∞), which are formulated in terms of the dispersal kernel and species growth rates in both directions. When …

The Role Of Nicotine, A7 Nicotinic Acetylcholine Receptors And Extracellular Matrix Remodeling In Pulmonary Fibrosis., Glenn Ward Vicary

Electronic Theses and Dissertations

The median survival for idiopathic pulmonary fibrosis (IPF) patients from diagnosis is a dismal 3 years. This condition is characterized by pulmonary fibroproliferation and excess production and disordered deposition of extracellular matrix (ECM) proteins resulting in obliteration of the original tissue architecture, loss of lung function and eventual death due to respiratory failure. The main hindrance to the development of effective treatments against pulmonary fibrosis is the late detection of its progression and is often of unknown cause. Tobacco smoke represents the most important environmental factor linked to the development of pulmonary fibrosis, with over 60% of IPF patients current …

The Effects Of Direct Peritoneal Resuscitation On Leukocyte Infiltration After Acute Brain Death., Jessica Lee Weaver

Electronic Theses and Dissertations

Background: Brain death triggers an inflammatory response that is associated with worse outcomes for recipients of transplanted organs procured from brain dead donors. The intestine has been shown to release inflammatory mediators in other types of shock, but its role is brain death is not well described. Direct peritoneal resuscitation (DPR) improves visceral organ blood flow and has been shown to reduce inflammation after hemorrhagic shock. Better understanding of this inflammatory process within the peripheral organs, the effects of DPR, and the role of the intestine could aid in better resuscitation of potential organ donors. Methods: Male Sprague-Dawley rats were …

Dietary Resource Utilization Patterns And Head Morphology Among Three Sympatric Watersnake Species., Micah Warren Perkins

Electronic Theses and Dissertations

The coexistence of similar species may be related to a variety of resource utilization differences. Dietary resource utilization variation may be the most important difference allowing for the coexistence of sympatric snake species. Many watersnakes (Nerodia spp.) live in sympatry and use similar aquatic habitats feeding on similar prey. While these sympatric watersnakes may have different general foraging patterns, snake diet may be affected by a variety of factors. Therefore, I initiated an investigation to understand the coexistence of sympatric plain-bellied (N. erythrogaster), diamondback (N. rhombifer) and northern (N. sipedon) watersnakes by addressing …

Using A Mouse Model To Investigate Male Bias In Autism Spectrum Disorder Incidence., Elizabeth Ann Gordon

Electronic Theses and Dissertations

The review (Chapter 1) overviews our current understanding of the sex bias in autism spectrum disorders (ASD), whereby males are 4 times more likely to develop the condition. The longstanding uncertainty surrounding the etiology of autism can be informed by inclusion of both sexes in future research to better understand the mechanism responsible for this sex bias. Both clinical studies and animal models are useful tools to elucidate the underlying cause(s) of sexual dimorphic morphological, physiological, and behavioral differences in the ASD phenotype. Sex differences in social behaviors are common across species, underscoring the importance of assessing baseline sex differences …

Pro-Inflammatory Cytokines Promote Viral Replication And Cell Cycle Mediators In Arenavirus-Induced Hepatitis., Gretchen E. Holz

Electronic Theses and Dissertations

Lassa virus (LASV) is an arenavirus and causative agent of Lassa fever (LF), a viral hemorrhagic fever in West Africa for which there is no vaccine. Lymphocytic choriomeningitis virus (LCMV), is used as a surrogate to mimic LASV-induced liver pathology. LCMV-WE, not LCMV-ARM, causes disease in primates and mice characterized by hepatitis, high viral load, hepatocyte proliferation, and upregulated proliferative triggers (e.g. TNF-α, IL-6 ). We hypothesize LCMV-WE induces pathological hepatocyte proliferation via pro-inflammatory triggers (TNF-α, IL-6) from macrophages, leading to: increased viral replication, modulated cell cycle, and arrested cell cycle. RAW 264.7 macrophages and AML-12 hepatocytes, were used as …

Establishing A Clinically Relevant Mouse Model Of Human Aml To Test Novel Transmethylation Inhibitors., Aditya Barve

Electronic Theses and Dissertations

Acute myeloid leukemia (AML) is a highly heterogeneous clonal disorder characterized by an accumulation of malignant immature myeloid progenitors in the bone marrow (BM) that hinder normal hematopoiesis. Patient AML exhibits a dramatic heterogeneity in terms of cytogenetics, disease morphology, and associated prognoses and/or chemotherapeutic sensitivity. Thus it becomes clearly evident that the investigation of novel therapeutics for AML will require model systems that are capable of recapitulating this stark heterogeneity in a patient specific manner. Furthermore, it is now understood that the surrounding bone marrow (BM) microenvironment and supporting cells play a critical role in leukemic progression as well …

Tnf-Like Weak Inducer Of Apoptosis (Tweak) : Not So Weak After All., Joseph Dekward Mcmillan Iv

Electronic Theses and Dissertations

Background: Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a proinflammatory cytokine belonging to the TNF super family. TWEAK produces a variety of cellular responses through the binding to fibroblast growth factor inducible 14 (Fn14), a member of TNF receptor superfamily. Although Fn14 lacks a death domain, TWEAK has been found to induce apoptosis in some cell types by perturbing the activity of certain pathways such as TNF-receptor signaling. TWEAK is also known to regulate proliferation and differentiation of myogenic cells. We have previously reported that the TWEAK-Fn14 system causes skeletal muscle wasting both in vitro and in …

Glutathione S-Transferase P In Glucose Homeostasis In Mice., Shubha Ghosh Dastidar

Electronic Theses and Dissertations

High calorie diets have fostered the current pandemic of obesity and comorbid conditions of non-alcoholic fatty liver disease (NAFLD), systemic insulin resistance (IR) and type 2 diabetes (T2D). Hepatic glutathione S-transferases (GSTs) are dysregulated in obesity, NAFLD and diabetes. The multifunctional GST pi isoform (GSTP) catalyzes acrolein metabolism and inhibits JNK (c-Jun NH₂-terminal kinase). The purpose of this study was to test specifically whether GSTP deficiency disturbs glucose homeostasis in mice. Hepatic GST proteins were downregulated by short-term high fat diet (HFD) in wild type (WT) mice concomitant with glucose intolerance, increased hepatic JNK activation and protein-acrolein …

Evaluating Cardiovascular Dysfunction During Increased Activity And Exercise Rehabilitation Following Incomplete Thoracic Spinal Cord Injury In The Adult Rat., Kathryn A. Harman

Electronic Theses and Dissertations

Spinal cord injury (SCI) results in whole-body dysfunction. While the majority of SCI research is focused on improving locomotor function after injury, cardiovascular (CV) disease continues to be the leading cause of morbidity in the chronic SCI patient population. The combination of injury-induced destruction of autonomic pathways, maladaptive plasticity within lumbosacral circuits, and the progressive decline in physical fitness contribute to the poor CV status of SCI individuals. Currently, there is little emphasis on implementing appropriately-timed acute rehabilitation techniques aimed to curtail maladaptive remodeling and improve CV outcomes. Furthermore, no pre-clinical or clinical studies have investigated the most appropriate time-course …

Distress, Circadian Rhythms, Immunity, And Survival In Presurgical Breast Cancer Patients., Whitney Nicole Rebholz

Electronic Theses and Dissertations

Women with breast cancer experience significant stress related to diagnosis and treatment, which may negatively affect physiology (e.g., circadian, endocrine, and immune) and contribute to cancer progression. Demographic (e.g., ethnic minority) and neighborhood factors can contribute to cancer-related stress. African American women with breast cancer experience more adverse disease pathology and earlier mortality; however, factors that explain these disparities rarely have been explored. It is hypothesized that psychosocial stressors (i.e., ethnicity, neighborhood stress, and cancerrelated stress) will be related to one another. Associations of psychosocial stress with physiological disruption are posited with regard to circadian, endocrine, and immune function. It …

Virginia Woolf’S Fictional Biographies, Orlando And Flush, As Prefigures Of Postmodernism, Jacob C. Castle

Electronic Theses and Dissertations

This thesis examines the way in which the fictional biographies of Virginia Woolf, Orlando and Flush, prefigure central tenets of postmodern fiction. To demonstrate the postmodern elements present in Orlando and Flush, this thesis focuses on how the fictional biographies exhibit three postmodern characteristics: concern for historiography, extensive use of parody, and the denaturalization of cultural assumptions. Born from Woolf’s desire to revolutionize biography by incorporating elements of fiction alongside historical fact, these two novels parallel later works of historiographic metafiction in several key respects. Woolf’s extensive use of parody in Orlando and Flush prefigures how postmodern parody …

Pharmacokinetics, Tissue Distribution, Synergistic Activity, And Antitumor Activity Of Two Isomeric Flavones, Crystal L. Whitted

Electronic Theses and Dissertations

Flavonoids are polyphenolic secondary metabolites found in plants that have bioactive properties including antiviral, antioxidant, and anticancer. Two isomeric flavone were extracted from Gnaphalium elegans and Achyrocline bogotensis, plants used by the people from the Andean region of South America as remedies for cancer. 5,7-dihydroxy-3,6,8-trimethoxy-2-phenyl-4H-chromen-4-one (5, 7–dihydroxy- 3, 6, 8 trimethoxy flavone/ flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy-2-phenyl-4H-chromen-4-one (3, 5–dihydroxy-6, 7, 8–trimethoxy flavone/ flavone B) have shown antineoplastic activity against colon cancer cell lines dependent upon their differentiation status. Pharmacokinetic studies reported herein were used to determine dosing for antitumor assays, as well as determine target tissue concentration. These included the …

Psychophysiological Analysis Of A Pedagogical Agent And Robotic Peer For Individuals With Autism Spectrum Disorders., Mohammad Nasser Saadatzi

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by ongoing problems in social interaction and communication, and engagement in repetitive behaviors. According to Centers for Disease Control and Prevention, an estimated 1 in 68 children in the United States has ASD. Mounting evidence shows that many of these individuals display an interest in social interaction with computers and robots and, in general, feel comfortable spending time in such environments. It is known that the subtlety and unpredictability of people’s social behavior are intimidating and confusing for many individuals with ASD. Computerized learning environments and robots, however, prepare a predictable, …

Heritage Tourism In Washington County, Tennessee: Linking Place, Placelessness, And Preservation, Chad F. Bailey

Electronic Theses and Dissertations

This thesis examines the formation of spatial theory and the linkage between space and place and their relationship with historic preservation and heritage tourism. First, this thesis analyzes the terms space and place, and how scholars define each term. Second, this thesis focuses on the concept of placelessness. Third, this thesis examines historic preservation as a strategy to help alleviate placelessness and as a crucial link to heritage tourism. This thesis also will use regional examples of preservation and tourism as exemplified by the preservation efforts of private organizations, citizens, and government officials in Jonesborough,Johnson City, and Washington County,Tennessee. This …

The Perceptions Of Northeast Tennessee Educators Regarding Arts Integration, Philip A. Wright

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine the perceptions of Northeast Tennessee Educators regarding arts integration. Specifically this study was an examination of the perceptions of district and K-8 school level administrators, K-8 general classroom teachers of math, science, social studies, or literacy, and K-8 arts specialists of dance, drama, music, or visual arts. Nine school districts in Northeast Tennessee agreed to participate in the study. Data were collected through an online survey system, SurveyMonkey.com. Data from 179 participants were used in the study. Seventeen items from the survey were measured on a 5-point Likert scale. Those items …

Structural And Functional Analysis Of Grapefruit Flavonol-Specific-3-O-Gt Mutant P145t, Sangam Kandel Mr

Electronic Theses and Dissertations

This research is focused on the study of the effect of mutating proline 145 to threonine on the substrate and regiospecificity of flavonol specific 3-O-glucosyltransferase (Cp3GT). While the mutant P145T enzyme did not glucosylate anthocyanidins, it did glucosylate flavanones and flavones in addition to retaining activity with flavonols. HPLC was used for product identification and showed mutant P145T glucosylated naringenin at the 7-OH position forming naringenin-7-O-glucoside and flavonols at the 3-OH position. Homology modeling and docking was done to predict the acceptor substrate recognition pattern and models were validated by experimental results. In other related work, …

Restoring Relationship: How The Methodologies Of Wangari Maathai And The Green Belt Movement In Post-Colonial Kenya Achieve Environmental Healing And Women's Empowerment, Casey L. Wagner

Electronic Theses and Dissertations

The effects of the colonial project in Kenya created multi-faceted damages to the land and indigenous people-groups. Using the lens of ecofeminism, this study examines the undergirding structures that produce systems such as colonization that oppress and destroy land, people, and other beings. By highlighting the experience of the Kikuyu people within the Kenyan colonial program, the innovative and ingenious response of Wangari Maathai's Green Belt Movement proves to be a relevant and effective counter to women's disempowerment and environmental devastation in a post-colonial nation. The approach of the Green Belt Movement offers a unique and accessible method for empowering …

Prudery And Perversion: Domination Of The Sexual Body In Middle-Class Men, Women, And Disenfranchised Bodies In Victorian England, Ashley Barnett

Electronic Theses and Dissertations

This research argues that with the rise of the middle-class, Victorian England saw the development of a power model in which middle-class men, middle-class women and disenfranchised bodies of children and lower-class women suffered from the demands of bodily domination. Because the bodily health of middle-class men was believed to represent national health, it was imperative that he dominate his body, particularly with regard to sexual urges. Consequently, the bodies of women with whom he sought sexual release suffered from forms of bodily domination as well. Through an analysis of journals and private writings of those living in Victorian England, …

The Handweavers Of Modern-Day Southern Appalachia: An Ethnographic Case Study, Cathryn F. Washell

Electronic Theses and Dissertations

One of the most prominent traditions associated with the Southern Appalachians is the art of weaving. Extensive research has focused on the history of Appalachian weaving, but there is little on the current weaving community. Today, the region still serves as an axis for weaving, and many practicing weavers, weaving instructors, and learning institutions can be found in Southern Appalachia. The core of this study is the interviews with ten weavers that reside and practice their work in Appalachia. Using concept coding, the transcripts of the interviews led to the development of four major themes that highlight the weavers’ discovery …

Determining Public Perceptions Toward Wildland Fire In The Veluwe Region Of The Netherlands, Amy Brennan

Electronic Theses and Dissertations

The Netherlands has been facing a growing threat of wildfires due to warmer

and drier weather patterns. The purpose of this study was to identify public

perceptions toward wildland fire in the forested Veluwe region of the country. The

Dutch have little experience with wildland fires or fire as a management tool. In a

collaborative effort between Stephen F. Austin State University and the Instituut

Fysieke Veiligheid, the Dutch public safety agency, a survey was distributed to

residents and visitors to the Veluwe to reveal and quantify public opinions and

perceptions regarding wildland fire and public expectations of government

agencies …

Residual Feed Intake And The Relationship With Performance, Temperament And Carcass Traits In Growing And Finishing Steers, Courtney R. Branton

Electronic Theses and Dissertations

Two trials were conducted with Santa Gertrudis steers, trial 1 (n = 115) and trial 2 (n = 118), were fed a roughage-based diet during the growing phase and high-grain diet during the finishing phase. Steers were weighed at 14-d intervals and dry matter intake (DMI) measured for 70 d during both phases. Residual feed intake (RFI) is the difference between actual DMI and DMI predicted from linear regression of DMI on mid-test metabolic body weight. Ultrasound carcass measurements were measured on day 0 and 70 of the growing phase. Steers were harvested after finishing phase and carcass cooler traits …

Breeding Season Avian Community Composition And Prey Availability In Eucalyptus And Slash Pine Plantations Of Southwestern Louisiana, Elizabeth J. Messick

Electronic Theses and Dissertations

The increased demand for wood products related to industries such as bioenergy and paper has resulted in a need for a consistent supply of raw materials. Eucalyptus (Eucalyptus spp.) plantations have the potential to boost wood production for pulpwood and biomass feedstocks. Species characteristics such as rapid, indeterminate growth, coppice regrowth, resistance to disease and insects, and tolerance of a range of environmental conditions make these species successful short-rotation woody crops (SRWCs). Camden white gum (Eucalyptus benthamii), a more cold tolerant species, has made management of eucalyptus plantations viable in southern portions of the United States such …

Effects Of Attachment Style, Coping Strategy, Social Support And Spirituality On Parental Grief Among Older Chinese Parents : Structural Equation Modeling., Yongqiang Zheng

Electronic Theses and Dissertations

Most modern grief theories and clinical working models are constructed in the context of Western culture, and the effectiveness or explanatory power of those theories and models have not been fully tested in other cultures, especially through a more complex interactive model. The current study focuses on the role of attachment style, coping strategy, social support and spirituality on older bereaved parents’ grief. It also explores if differences in age, gender, causes of the child’s death, time since the death have a differential effect on parental grief. The purpose of this study was to test the main factors effectively mediating …

The Available Means Of Imagination : Personal Narrative, Public Rhetoric, And Circulation., Stephanie D. Weaver

Electronic Theses and Dissertations

This dissertation examines the digital circulation of personal narratives by non-celebrity individuals that become part of larger public and political debates. I posit the “available means of imagination” to describe the ways that narratives – cultural, fictional, and personal – influence our ability to understand the many facets of a given public debate before tracing the interactions among narrative, emotion, and circulation in a series of case studies using new materialist methods. I argue that emotion plays a key role in structures of participation of social media and in how we subsequently engage with contemporary political issues, especially with regards …

Sex, Sexuality And Sexual Practice For Trans Individuals And Their Romantic Partners., Kinton Rossman

Electronic Theses and Dissertations

Transgender populations demonstrate significant sexual diversity, with over three-fourths (77%) of transgender individuals identifying as sexual minorities (Grant et al., 2011); however, a majority of research on sex, sexuality, and sexual practice has focused exclusively on cisgender and heterosexual individuals and, perhaps more importantly, addressed these issues a heteronormative and cisnormative perspective (Nichols, 2014). Such frameworks have created significant gaps in research about sex, sexuality, and sexual practice for trans individuals including understanding how gender transition influences sex and sexuality and how trans individuals and their partners engage in sexual practice. The current study addressed these omissions in the literature …

Memory And Fine Motor Skill Test Performance Among Children Living Near Coal Ash Storage Sites., Lindsay Koloff Tompkins

Electronic Theses and Dissertations

Coal ash, a byproduct of coal combustion,is reduced in 47 U.S. states andfrequently contains heavy metals, some of which are known neurotoxins. An estimated 1.5 million children live near sites where coal ash is produced andstored, yet there have been no studies assessing coal ash exposure and children’s neuro behavioral health. This study is part of a larger cross-sectional study, Coal Ash and Neuro behavioral Symptoms in Children Aged 6- 14 Years Old, and aimed to determine the relationship between children’s memory and fine motor skill test performance and the proximity of the home to coal ash storage sites, the …