Digital Commons Network^™

Using Molecular Genetic And Demographic Tools To Improve Management Of Ex Situ Avian Populations, Gina Ferrie

Electronic Theses and Dissertations

Small populations, specifically those that are isolated from others, are more prone to extinction than larger inter-connected populations. The risks that these small isolated populations face include loss of genetic diversity due to founder effects and inbreeding due to population bottlenecks, as well as demographic uncertainty due to fluctuating fecundity and mortality rates and impacts of external environmental factors. Ex situ populations, including those managed as conservation breeding programs with species recovery aims, as well as those that do not have reintroduction goals but are managed for long term population sustainability, suffer from the same extinction risks as small and …

Development Of Molecular Diagnostic Tools For Mycobacterium Species, Hillary Bengtson

Electronic Theses and Dissertations

This dissertation focuses on the development of diagnostic tools for mycobacteria using hybridization based technologies including binary deoxyribozyme (BiDz) sensors and microarrays. The genus Mycobacterium, is a diverse group of bacteria containing 150+ species including M. tuberculosis (M.tb) and non-tuberculous mycobacteria (NTM) which exhibit a range of pathogenicity, drug susceptibility and growth characteristics. M. tuberculosis (M.tb) is the causative agent of tuberculosis (TB) and the leading cause of infectious disease related deaths worldwide. The control of TB is limited by the lack of sensitive and specific diagnostic tools available at the point of care (POC). The studies presented here illustrate …

Natural Disasters, Rebecca Cobb

Electronic Theses and Dissertations

Natural Disasters is a collection of twelve short stories that feature a variety of narrators as they interact with the ones they love. In these stories, characters experience puberty, friendship, love, loss, trauma, and the everyday magic of living as they fight to master their own failings. Those lucky enough find solace in the forgiving beauty of nature, while others succumb to the untamable power of its disasters. This thesis is useful, important, and unique as it focuses on the stories of a variety of characters, mostly women and children, and displays the beauty and fearsome power of nature as …

Targeted Therapy Development For Neurofibromatosis Type 2, Marisa Fuse

Electronic Theses and Dissertations

Neurofibromatosis type 2 (NF2) is a debilitating disease characterized by the formation of bilateral vestibular schwannomas, which compress the vestibulocochlear nerve and cause deafness. Additional peripheral schwannomas, meningiomas and ependymomas may also form. NF2 is caused by mutations in the NF2 gene, resulting in the loss of function of the merlin tumor suppressor. Merlin functions in multiple signaling pathways and its absence in Schwann cells yields increased cell survival and proliferation, thereby causing schwannoma formation. First line treatment for NF2 is watchful waiting and surgical removal of tumors, potentially resulting in facial paralysis and deafness. To date, there are no …

Reproductive Life History And Signal Evolution In A Multi-Species Assemblage Of Electric Fish, Joseph Waddell

Electronic Theses and Dissertations

Animals that co-occur in sympatry with multiple closely-related species use reproductive mate attraction signals not only to assess the quality of a potential conspecific mate (sexual selection), but also to discriminate conspecifics from heterospecifics (species recognition). However, the extent to which sexual selection and species recognition may interact, or even conflict, is poorly known. Neotropical electric fish offer unrivaled opportunities for understanding this problem. They generate simple, stereotyped mate attraction signals that are easy to record and quantify, and that are well-understood from the neurobiological perspective. Additionally, they live in electrically-crowded environments, where multiple congeners live and reproduce in close …

Women In White: My Journey Into Color, Madison Tarbox

Electronic Theses and Dissertations

In many religious circles white is a symbol for purity, cleanliness, and perfection. However, white is also synonymous with empty, blank, and colorless. Women in White: My journey into color is a project centralized around a personal study of the cultural pressures of "perfection" presented both in religious cultures and in the every-day life of a performer. Utilizing the catalyst of a cabaret-style performance, Women in White explores the struggles of nine different female characters in the musical theatre cannon and aims to draw a personal connection from their journey. Inspired by the wise words of my own mother, this …

Exploring Interactions Between Adult English Learners And Their Teachlive Digital Character Peers, Ting Yan

Electronic Theses and Dissertations

Peer interaction is an important part of language learning. The results of previous studies showed advantages and disadvantages of peer interaction between native speakers and English learners. Using the educational simulation platform TeachLivE as the interaction platform, this qualitative descriptive case study explored the possibilities of bridging the gap between classroom language practice and real-life second language communication. The study recruited eight participants for a task-based interaction project. Using conversation analysis, the study revealed details in communication between adult English learners and their digital character peers. During the interactions, the participants were actively involved and the most frequent communication patterns …

The Effect Of Artificial Light Pollution On Orientation Of Hatchling Loggerhead Sea Turtles (Caretta Caretta) In The Grand Strand Region, South Carolina, Emily S. Asp

Electronic Theses and Dissertations

Sea turtle hatchlings primarily utilize sight to detect differences in elevation and light intensity present along the horizon to navigate from the nest to the water’s edge. The addition of artificial lights can cause visual misdirection, resulting in disorientation (aimlessly wandering in circular paths) or misorientation (moving in distinct paths away from ocean). Extensive research has been done on effects of high levels of artificial light but little on effects of comparatively lower levels of artificial light on hatchling sea turtle orientation. This study examined these lower intensity areas to identify if there is a threshold of artificial light above …

The Spatial Ecology Of The Southern Copperhead In A Fragmented And Non-Fragmented Habitat, Megan Veronica Novak

Electronic Theses and Dissertations

Habitat fragmentation may alter the spatial ecology of organisms inhabiting the fragmented landscape by limiting the area of habitat available and altering microhabitat features. I quantified and compared movement of southern copperheads (Agkistrodon contortrix contortrix) in a fragmented and non-fragmented habitat to determine the effects of habitat fragmentation on the spatial ecology of the southern copperhead. Effective distance moved by each individual was measured by the use of thread bobbins attached via medical tape to the posterior quarter of the snake. Straight-line distance moved and occupied area were calculated with the GPS coordinates recorded at each snake sighting. Straight-line distance …

Sources And Long-Term Trends In Fecal Bacteria Contamination In The Fresh And Marine Waters Of The Grand Strand, Cara E. Schildtknecht

Electronic Theses and Dissertations

Microbial water quality is of significant concern in the two watersheds of the Grand Stand. Polluted runoff, malfunctioning septic tanks, and centralized sanitary sewer failures are common sources of fecal bacteria contamination in coastal areas and pose a threat to human health through recreational use of waterbodies and shellfish consumption. Volunteer water quality monitoring programs are crucial in expanding upon assessments of fecal bacteria contamination by regulatory monitoring. Bacteria monitoring data collected by volunteers and Coastal Carolina University’s Environmental Quality Laboratory has been used to identify sites to be investigated by microbial source tracking. Microbial source tracking has been used …

A Chemical And Genetic Approach To Study The Polyamine Transport System In Drosophila, Minpei Wang

Electronic Theses and Dissertations

Polyamines are small cationic molecules that play important roles in most vital cellular processes including cell growth and proliferation, regulation of chromatin structure, translation and programmed cell death. Cellular polyamine pools are maintained by a balance between biosynthesis and transport (export and import). Increased polyamine biosynthesis activity and an active transport system are characteristics of many cancer cell lines, and polyamine depletion has been shown to be a viable anticancer strategy. Polyamine levels can be depleted by α-difluoromethylornithine (DFMO), an inhibitor of the key polyamine biosynthesis enzyme ornithine decarboxylase. However, malignant cells often circumvent DFMO therapy by up-regulating polyamine import; …

Development Of Binary Composite Solid Microparticles For Improved Intranasal Absorption Of Low Permeation Drug Molecules, Dipy Madhukar Vasa

Electronic Theses and Dissertations

The objective of this work was to introduce a novel formulation strategy for intranasal delivery of two low permeation candidates: levodopa (L-DOPA; antiparkinsonism drug) and ribavirin (RBVN; an antiviral drug). The central hypothesis states that preparation of binary composite microparticles with Pluronic® triblock copolymers (polyethylene glycol-polypropylene glycol-polyethylene glycol) should allow increased drug transport across nasal mucosal tissue relative to drug alone. Testing the hypothesis necessitated execution of specific aims directed to addressing issues inherent to the proposed approach. The work herein is presented with respect to two separate subjects: (1) material understanding and (2) formulation development. In the first two …

Becoming A Priestly People: A Biblical Theology Of Liturgical Sacrifice As Spiritual Formation, Ximena Debroeck

Electronic Theses and Dissertations

The centrality of sacrifice in the Judeo-Christian tradition is undeniable; nevertheless, unidimensional presentations of sacrifice have contributed to an incomplete, and at times distorted understanding. This work does not aim to resolve every ambiguity or misunderstanding, rather, it presents a canonical approach interpretation and brings attention to three underappreciated dimensions of sacrifice: first, the relationship between the external actions of the ritual and the internal dynamic of spirituality and morality; second, the reality that sacrifice refers not only to death, but to a way of living; and third, atonement and reconciliation are not the only reasons to offer sacrifice. My …

Altruism, Autonomy, And Human Dignity For The Sustainability Of Post-Mortal Organ Donation, Aiyub Mohammad Fahad Alwehaibi

Electronic Theses and Dissertations

In most countries, the shortage for transplant organ sources has become common due to the significant increase in number of awaiting organ recipients, which, in turn, urges countries develop systemic programs in order to increase the number of organ donors and resolve their problems. Generally, either living or deceased organs are accepted in transplant practice, yet, each source holds its own benefits and adverse effects. Living organ donation encounters controversial and problematic issues at individual, social, and humanity levels. Cadaveric organ donation faces the obstacles of the criteria of death, which vary from cultural to medical perspectives, and the effective …

3d Bioprinting Hydrogel For Tissue Engineering An Ascending Aortic Scaffold, Benjamin Stewart

Electronic Theses and Dissertations

The gold standard in 2016 for thoracic aortic grafts is Dacron^®, polyethylene terephthalate, due to the durability over time, the low immune response elicited and the propensity for endothelialization of the graft lumen over time. These synthetic grafts provide reliable materials that show remarkable long term patency. Despite the acceptable performance of Dacron^® grafts, it is noted that autographs still outperform other types of vascular grafts when available due to recognition of the host's cells and adaptive mechanical properties of a living graft. 3-D bioprinting patient-specific scaffolds for tissue engineering (TE) brings the benefits of non-degrading synthetic …

An Integrated Archaeological Investigation Of Colonial Interactions At A Seventeenth-Century New England Site, Maeve E. Herrick

Electronic Theses and Dissertations

The focus of this research is the ways in which interactions between Indigenous peoples and English settler-colonists were manifested in the landscape at a seventeenth-century site in South Glastonbury, Connecticut. Magnetometry and ground-penetrating radar allowed for the location of anthropogenic and geological features on the landscape, and for the seventeenth-century landscape to be recreated. This reconstruction indicated that Europeans and Indigenous peoples may have been cohabitating the site. Archival research helped to uncover what types of interactions may have been occurring at the site. Excavations uncovered "Indigenous" artifacts in a "European" context, leading to the reconsideration of the prevailing perspectives …

An Ensemble Study Of The Sea Level Rise Impact On Storm Surge And Inundation In The Coastal Bangladesh, Mansur Ali Jisan

Electronic Theses and Dissertations

The hydrodynamic model Delft3D is used to study the impact of Sea Level Rise (SLR) on storm surge and inundation in the coastal region of Bangladesh. To study the present-day inundation scenario, track of two known tropical cyclones (TC) were used: Aila (Category 1; 2009) and Sidr (Category 5; 2007). Model results were validated with the available observations. Future inundation scenarios were generated by using the strength of TC Sidr, TC Aila and an ensemble of historical TC tracks but incorporating the effect of SLR. Since future change in storm surge inundation under SLR impact is a probabilistic incident, that’s …

An Exploratory Study On The Possible Effect Of Hydraulic Fracturing Activities On Air Quality In The Eagle Ford Shale Region Of Texas., Laura B. Fimibama

Electronic Theses and Dissertations

Eagle Ford Shale (EFS) play is one out of five major oil and gas formations in Texas, found in the South-Eastern region with approximately 30 counties. In addition to natural gas, it produces crude oil and petroleum liquids which in recent times has resulted in a surge in drilling due to a new drilling technology – hydraulic fracturing that injects massive quantities of water along with sand and chemical additives into a well to create tiny hairline cracks in rock formations. The success of this technology has made gas extraction more economically feasible. However, this technique has generated an array …

Cloning And Expression Of Bovine Alpha S1 Casein Peptides Of Variable Size And Position Of Sppep Inhibitor Sequence, Avleen Kaur Vishram

Electronic Theses and Dissertations

Eurygaster integriceps Puton (Sunn pest) is the most damaging insect pest of wheat in areas of North Africa, Eastern Europe and Western and Central Asia. Sunn pest injects an enzyme characterized as a prolyl endylprotease (spPEP) into wheat grains that digests the gluten protein (Darkoh et al., 2010). Previous studies have shown that casein peptides generated by Lactobacillus species have inhibitory effects on human (hPEP) and bacterial prolyl endoproteases (bPEP) which makes casein a good natural candidate to inhibit spPEP. Developing biopesticides for food products from αS1 casein is complicated due to some allergenic amino acid sequences. Ruiter et al …

Tobacco Use, Cessation, And Locus Of Control Among College Students, Bernard Ambe

Electronic Theses and Dissertations

ABSTRACT

The purpose of this study was to (a) determine the locus of control among American college students (b) determine if tobacco use or cessation correlate with any demographic variables to better understand the efficacy of tobacco interventions and help design an intervention most effective in the prominent LOC of college students.

The Global Adult Tobacco Survey (GATS) was modified for the purposes of this study and used to determine demographic factors and tobacco usage status. The modified GATS survey also included the LOC questionnaire which yielded the data. Seventy-four responses were recorded. The mean locus of control scores were …

Effect Of Reproductive Efficiency In Regards To Workload On Peripheral Circulations Of Blood Cortisol And Leptin In The Open Mare, Michaelle K. Coker

Electronic Theses and Dissertations

ABSTRACT

Effect of Reproductive Efficiency in Regards to Workload on Peripheral Circulations of Blood Cortisol and Leptin in the Open Mare

(December, 2016)

Michaelle Kathleen Coker, B.S., Texas A&M University

Thesis Director: Dr. John Michael Mehaffey

Quarter and Paint horse mares (n= 9) were randomly assigned to 1 of 3 groups after being blocked based by expected conception date. Groups consist of mares on no work (NW), light work (LW), and moderate work (MW). Blood was taken weekly via jugular venipuncture prior to first heat cycle from each mare. Additionally, blood was taken at time of ovulation and then weekly …

The Influence Of The Invasive Chinese Tallow (Triadica Sebifera) Leaf Litter On Aquatic Chemistry And Microbial Community Composition, Raymond D. Montez

Electronic Theses and Dissertations

Global climate change and anthropogenic activity have facilitated the movement and invasive potential of nonnative plants in native environments. These invasions can have negative effects on ecosystem diversity and function. The nonnative and invasive plant, Chinese Tallow (Triadica sebifera), has already invaded much of the south eastern US where it is outcompeting native tree species and changing ecosystem diversity in a variety of habitats. Leaf litter from the Chinese tallow has been shown cause changes in dissolved oxygen and pH in the aquatic environment. Turbidity is also affected when Chinese tallow litter is present in water. A series of …

Computational Methods For The Analysis Of Functional 4d-Ct Chest Images., Ahmed Soliman Naeem Soliman

Electronic Theses and Dissertations

Medical imaging is an important emerging technology that has been intensively used in the last few decades for disease diagnosis and monitoring as well as for the assessment of treatment effectiveness. Medical images provide a very large amount of valuable information that is too huge to be exploited by radiologists and physicians. Therefore, the design of computer-aided diagnostic (CAD) system, which can be used as an assistive tool for the medical community, is of a great importance. This dissertation deals with the development of a complete CAD system for lung cancer patients, which remains the leading cause of cancer-related death …

Transient Receptor Potential Cation Channel, Subfamilies V, Member 1 (Trpv1) And M, Member 1 (Trpm1) Contribute To Neural Signaling In Mouse Retina., Jennifer Noel

Electronic Theses and Dissertations

The retina processes light information through parallel pathways in order to extract and encode the visual scene. Light information is transmitted to the brain through approximately 30 ganglion cells (GCs), the retinal output neurons. Trp channels modulate the responses of retinal neurons within specific pathways. The study of the expression and function of the majority of Trp channels in the retina is largely in its infancy. My dissertation first investigated the expression and function of the transient receptor potential vanilloid-1 (TRPV1) receptor/channel in the retina. TRPV1, the first cloned and most highly studied Trp channel in the peripheral nervous system, …

Examination Of The Relevance Of Pi3k And Pten Pathway In Ustilago Maydis., Nathaniel Edward Dalton

Electronic Theses and Dissertations

Ustilago maydis is a fungal pathogen that is an obligate parasite on Zea mays (Corn) in order to undergo and complete its sexual life cycle. The cells grow as a haploid form until they meet a compatible partner and undergo a dimorphic switch from a budding yeast-like cell type to a filamentous dikaryon. Infection of the host plant is characterized by leaf chlorosis, anthocyanin content, and formation of large galls that develop on the stem, leaves, cobs, and flowers. Many signaling cascades such as MAPK and PKA are involved in the regulation of the infection lifecycle. The Phosphatidylinosotiol 3 Kinase/ …

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal

Electronic Theses and Dissertations

Unusual monoenoic fatty acids (UMFA’s) and specialized metabolites called anacardic acids (AnAc) are produced in glandular trichomes of Pelargonium ´ hortorum (geranium). The UMFA’s, 16:1^∆11 and 18:1^∆13 are precursors for the synthesis of unsaturated AnAc 22:1n5and 24:1n5 that contribute to pest resistance in geraniums. UMFAs and their derived AnAc metabolites not only provide a useful biological marker that differentiates the biosynthetic pathway for unusual mononenes from the common fatty acids (i.e. stearic, palmitic, oleic, linoleic and linolenic) but also have industrial, medical and agricultural applications. Fatty acid biosynthesis enzymes like acyl carrier proteins (ACPs); thioesterases (TEs) and β-ketoacyl-ACP …

The Zodiac Army., Keith Charles Marks

Electronic Theses and Dissertations

This thesis is the beginning of a book title The Zodiac Army. The book is the first in a trilogy and follows three different characters. The first, Ash, is a teenage boy who discovers he is a powerful magician who, along with another magician, can control the elements of nature. The two form a Twin pairing and will fight for each other and for the safety of the kingdom. Alora, the second character, is a young and faithful priestess in the Temple. Her faith, in both the gods and those in charge of the Temple, could bring the entire system …

Retinoic Acid Signaling Regulates Krt5 Independently Of Stem Cell Markers In Submandibular Salivary Gland Epithelium., Timur Maratovich Abashev

Electronic Theses and Dissertations

Vitamin A metabolism, which produces the signaling molecule Retinoic Acid (RA), has been demonstrated to be important for growth and branching morphogenesis of mammalian embryonic salivary gland epithelium. However, it is not known whether RA functions directly within epithelial cells or in associated tissues that influence morphogenesis of salivary epithelium. Moreover, downstream targets of RA transcriptional regulation have not been identified. Here we show that canonical RA signaling occurs in multiple tissues of embryonic mouse salivary glands, including epithelium, associated parasympathetic ganglion neurons, and non‑neuronal mesenchyme. By culturing epithelium explants in isolation from other tissues we demonstrate that RA influences …

The Role And Regulation Of Inhibitor Of Kappa B Kinase In The Impaired Monocyte Response., Norman James Galbraith

Electronic Theses and Dissertations

Trauma, major surgery and systemic infection can lead to a subsequent immunosuppressed state which can predispose patients to nosocomial infection and death. Approximately a sixth of patients will have impaired monocyte function, as determined by decreased HLA-DR expression or decreased TNF-α production in response to ex-vivo lipopolysaccharide (LPS) stimulation. This project aimed to develop an in-vitro model of impaired monocyte function using isolated monocytes from healthy volunteers, to study the effect of monocyte impairment on IκK signaling, and determine the role of IκK in the impaired monocyte response. These studies have provided the following results:

1. Monocytes treated with low dose …

Secular But Not Superficial : An Overlooked Nonreligious/Nonspiritual Identity., Daniel G. Delaney

Electronic Theses and Dissertations

Since Durkheim’s characterization of the sacred and profane as “antagonistic rivals,” the strict dichotomy has been framed in such a way that “being religious” evokes images of a life filled with profound meaning and value, while “being secular” evokes images of a meaningless, self-centered, superficial life, often characterized by materialistic consumerism and the cold, heartless environment of corporate greed. Consequently, to identify as “neither religious nor spiritual” runs the risk of being stigmatized as superficial, untrustworthy, and immoral. Conflicts and confusions encountered in the process of negotiating a nonreligious/nonspiritual identity, caused by the ambiguous nature of religious language, were explored …