Digital Commons Network^™

Role Of Tlrs, Hippo-Yap1 Signaling, And Micrornas In Cardiac Repair And Regeneration Of Damaged Myocardium During Ischemic Injury, Xiaohui Wang

Electronic Theses and Dissertations

Cardiovascular disease is a leading cause of death in the United States. Toll-like receptor (TLR)-mediated pathways have been demonstrated to play a role in myocardial ischemia/reperfusion (I/R) injury. We and others have shown that PI3K/Akt signaling is involved in regulating cellular survival and protecting the myocardium from I/R induced injury. In this dissertation, we provide compelling evidence that miR-125b serves to “fine tune” TLR mediated NF-kB responses by repressing TNF-a and TRAF6 expression. We constructed lentiviral expressing miR-125b, delivered it into the myocardium. The data showed that delivery of lentivirus expressing miR-125b significantly reduces myocardial infarct size and improves cardiac …

Enemy Exacerbation: Effects Of Predator Stress On Sulfate Lethality In Freshwater Amphipods (Gammarus Minus), Trevor Chapman

Electronic Theses and Dissertations

Predator cues can influence how aquatic organisms respond to anthropogenic contaminants. This study examined the effects of predator cues on behavior, metabolic rate, and sulfate (as Na₂SO₄) toxicity in amphipods (Gammarus minus). Predator cues included alarm cue (macerated conspecifics) and kairomone from mosquitofish (Gambusia affinis). Amphipods decreased activity and increased time in refuge when exposed to alarm cue, and increased time in refuge when exposed to kairomone. While median lethal concentrations (96-h LC₅₀) were not influenced by predator cues, analysis of dose response curves indicated that kairomone exposure increased amphipod …

Vulnerability Assessment Of Groundwater To No3 Contamination Using Gis, Drastic Model And Geostatistical Analysis, Adela Beauty Adu Agyemang

Electronic Theses and Dissertations

The study employed Geographical Information System (GIS) technology to investigate the vulnerability of groundwater to NO₃ content in Buncombe County, North Carolina in two different approaches. In the first study, the spatial distribution of NO₃ contamination was analyzed in a GIS environment using Kriging Interpolation. Cokriging interpolation was used to establish how NO₃ relates to land cover types and depth to water table of wells in the county. The second study used DRASTIC model to assess the vulnerability of groundwater in Buncombe County to NO₃ contamination. To get an accurate vulnerability index, the DRASTIC parameters were …

Get Thee To A Nunnery: Unruly Women And Christianity In Medieval Europe, Sarah E. Wolfe

Electronic Theses and Dissertations

This thesis will argue that the Beowulf Manuscript, which includes the poem Judith, Saxo Grammaticus’s Gesta Danorum, and the Old-Norse-Icelandic Laxdæla saga highlight and examine the tension between the female pagan characters and their Christian authors. These texts also demonstrate that Queenship grew fragile after the spread of Christianity, and women’s power waned in the shift between pre-Christian and Christian Europe.

Effects Of Lactobacillus Rhamnosus Mrs6an On Intestinal Gpat3 Pathway Involved In The Lipid Transport In Enterocytes, Ayodeji Oludare Ipinmoroti

Electronic Theses and Dissertations

Accumulation of triglyceride (TG) in enterocytes of the small intestine follows two specific absorption pathways: the monoacylglycerol acyltransferase pathway (MGAT) and glycerol acyltransferase pathway (GPAT), the latter (GPAT) is usually found in the small intestine. In this study, we investigated the effects of Lactobacillus rhamnosus MRS6AN, an isolate from “amabere amaruranu” a Kenyan traditional cultured milk, on triglyceride accumulation and expression of GPAT3, I-FABP, MTP and NPC1L1 in Caco-2 cell enterocyte model. Intracellular triglyceride level (TG) of Caco2 cells was significantly reduced by live L. rhamnosus (LB) compared with other bacterial products. MTP expression in Caco2 cells was minimally reduced …

A Study Of Salmonid Growth In Two Southern Appalachian Headwater Streams, Joshua Argo

Electronic Theses and Dissertations

This study sampled salmonid populations in two headwater streams in East Tennessee, Briar Creek and Left Prong Hampton Creek. Length and weight data were used to calculate the growth of these populations to determine if significant variation exists between isolated brook trout populations. Slope comparisons concluded that there was a difference in growth between brook trout populations of these streams (p

Cultivar, Season Extension, Fertilizer Trials Of Swiss Chard And Pedagogy Of Assessing Cultivars Using Technology, Chanelle Angeny

Electronic Theses and Dissertations

Abstract. Diversity of species is a cornerstone of horticulture, and the constant stream of new plant cultivars broadens the palette of options available to the industry. Fifteen Swiss chard (Beta vulgaris var. cicla) cultivars were evaluated during the fall/winter of 2014-2015 season (season 1) and 27 cultivars during the fall/winter of 2015-2016 season (season 2) to determine the best performing cultivars for production and landscape use for east Texas and the southeast by assessing survivability and yield. There was an increase in production of 3.0 ± 0.5 g per plant per harvest under the low tunnel. The cultivar …

Effect Of Protease Supplementation In Broiler Feed On Growth Performance, Carcass Yield And Total Nitrogen Retention In Fecal Matter And Litter, Jawad Al-Juboori

Electronic Theses and Dissertations

ABSTRACT

The objectives of this study were to determine the effects of protease supplementation on commercial broiler performance, carcass yield, and nitrogen retention in fecal matter and litter. Total of 4,800 female (Ross 708) birds split into 96 floor pens, and randomly assigned to one of four treatment groups. Birds were placed within 96, 5’x10’ floor pens in a randomized-block design at the SFASU Poultry Research Center. Birds were randomly divided among the pens at a stocking density of 1.00 ft2/bird (50 birds/pen*24 pens/treatment=1200 birds/treatment), and reared for 49 days on used pine shavings. The target average weight for the …

Quality And Yield Responses Of Seven Warm Season Forage Grasses To Partial Shading In A Simulated Silvopasture In East Texas, Jodi E. Hill

Electronic Theses and Dissertations

The goal of this project was to evaluate growth and nutritional characteristics of seven forages, including various warm season native grasses, grown under simulated partial shading (50%) typical of a loblolly pine silvopastoral system in East Texas. In order to meet the overall objective, slatted shade structures were constructed that simulated the quantity, quality, and overall light regime found beneath loblolly pine stands arranged for silvopasture. The forages selected for the study included ‘Tifton 9’ bahiagrass (Paspalum notatum), ‘Tifton 85’ bermudagrass (Cynodon dactylon), ‘Alamo’ switchgrass (Panicum virgatum), ‘Kaw’ Big Bluestem (Andropogon gerardii), ‘Americus’ …

Design Plan For The Sawmill Town History Wing At The Texas Forestry Museum, Kendall D. Gay

Electronic Theses and Dissertations

The Texas Forestry Museum in Lufkin, Texas is the only forestry museum in the state. It preserves artifacts and educates visitors about Texas’ forest industry history. The museum has a Sawmill Town History Wing that is outdated and in need of a refreshing exhibit design based on current best practices. Using a previous museum audit as a guide, the new exhibit will have better flow, panel aesthetics, content, and interactive elements. By creating a new exhibit, the museum is better able to educate and entertain the visitors about Texas’ forest industry history.

The Regulation Of Rotavirus–Infected Ht29.F8 And Ma104 Cells Treated With Arachidin 1 Or Arachidin 3, Caleb M. Witcher

Electronic Theses and Dissertations

Rotavirus (RV) infections cause severe life threatening diarrhea in young children and immunocompromised individuals. Several effective vaccines have been developed for young children but are not protective against all strains of RV, and there are no anti-RV therapeutics. Our laboratory has discovered a decrease in the number of infectious simian RV particles (SA114f) in human intestinal cell line, HT29.f8 cells with the addition of either of two stilbenoids, arachidin-1 (A1) or arachidin-3 (A3). This suggests effects on the host cell and RV replication. We examined the cellular effects of human RV strain (Wa) on a human intestinal cell line (HT29.f8) …

Racial Socialization And Its Moderating Effects On Microaggressions, Jusiah L. Prowell

Electronic Theses and Dissertations

The purpose of this research was to examine the relationship between racial socialization, and how it mitigates the distress caused by microaggressions. Although overt racism is no longer socially acceptable, racism still occurs in a covert form called microaggressions. Research has shown that microaggressions causes negative effects on its’ victims the same as overt racism. Also, literature has shown microaggressions in the classroom and on college campuses cause feelings of isolation and inferiority in college students. Racial socialization is the process of being immersed in one’s culture to strengthen against negative factors. The construct of racial socialization is the idea …

After The Shoe Fits: A Rhetorical Analysis Of Four Versions Of The Cinderella Narrative, Faith L. Boren

Electronic Theses and Dissertations

Fairy tales hold the power to influence societies and to challenge societal injustices, and the story of Cinderella exemplifies both of these roles. In this study, I conduct a rhetorical analysis of four different versions of the Cinderella narrative: Charles Perrault’s “Cendrillon,” the Brothers Grimm’s “Ascenputtel,” Anne Sexton’s "Cinderella,” and Disney’s Cinderella (2015). I examine Perrault’s “Cendrillon” and the Grimms’ “Aschenputtel” using constitutive rhetoric. This theory operates around the basic premise that rhetoric holds the power to aid in the shaping of societies. While analyzing “Cendrillon” and “Aschenputtel,” I specifically look for themes of classism and nationalism, respectively. I then …

Therapy Dogs And The Impact On Employees In The Pediatric Medical Setting, Laine Foith

Electronic Theses and Dissertations

More than 40% of nurses reported experiencing significant burnout. Burnout is characterized by disengagement, cynicism, negative views of personal accomplishment and ability, and emotional exhaustion. The healthcare providers that experience burnout can possibly expect a decrease in ability to recognize/report errors, increase of negative feelings toward the patient, and decrease levels of patient satisfaction (Ernest, 2014). One of the ways Schub (2015) suggested to regulate burnout for employees was to provide psychosocial support to colleagues to reduce stress. This study is one of the first attempts to bridge the gap between the unknown correlation between qualitative and quantitative benefits of …

Uncovering Meaning In Montessori Teachers’ Lived Experiences Of Cosmic Education As A Tool For Social Justice, John Allen Branch

Electronic Theses and Dissertations

This inquiry focused on the lived experiences of Montessori teachers in implementing Montessori’s Cosmic Education as a tool for social justice in their classrooms in order to more fully understand Cosmic Education’s meaning, purpose, and practice. The researcher also sought to understand how Cosmic Education could be an effective pedagogy of place, providing historical and social contexts in which students may develop and grow. The study used a post-intentional phenomenological design (Vagle, 2014), and was based on a series of interviews with five Montessori teachers from different classroom age levels. The data were analyzed using poetic inquiry through the form …

Cloning, Purification, And Biochemical Characterization Of Human Prolyl Endopeptidase, Travis K. Moore

Electronic Theses and Dissertations

Eurygaster integriceps Puton, common name sunn Pest, is one of the primary sources of wheat crop wastes in North Africa, Middle East, and Eastern Europe. It feeds by injecting the wheat grain with an enzyme characterized as prolyl endoprotease (spPEP) that breaks down Gluten, the wheat’s main constitutive protein necessary for bread production (Darkoh et al., 2010). Previously, it has been shown that peptides isolated from Lactobacillus hydrolysates of caseins in bovine milk are able to inhibit mammalian PEP in colon cells, as well as bacterial PEP (Juillerat-Jeanneret et al., 2010). While recombinant versions of these peptides are also potential …

Does Touch Have A Place In Interpretation? Researching The Role Of Appropriate Interpersonal Touch (Ait) In Interpretive Programs, Mickey Shortt Jr.

Electronic Theses and Dissertations

The role of interpersonal touch has been studied in communication fields, demonstrating a handshake or other form of appropriate interpersonal touch (AIT) has rendered favorable results in rapport building with an audience as well as developing audience comfort and receptivity to messaging. This study aimed to determine if the use of a handshake prior to interpretive programming would increase the positivity of perception of a visitor toward interpreters and/or the message shared by interpreters. Researchers also sought the viewpoints of professionals in the field of interpretation about the current use and perception of touch. Finally, a post-survey interview was conducted …

Challenges To Establishing Oaks And Pecan In Bottomland Forests In The Post Oak Savannah And Blackland Prairie, Luke B. Oliver

Electronic Theses and Dissertations

Removal and degradation of bottomland hardwood forests (BLHW) in the southern United States has been extensive over the past 200 years. Large mast-production is crucial to the survival of some high-priority wildlife. A two-year study monitored survival and growth of planted seedlings receiving different levels of overhead density reduction and competition control. Competition control did not increase survival of the oak species. Pecan seedlings receiving the weed barrier mat treatment had greater survival than pecan seedlings receiving the herbicide treatment, or those receiving no competition control. A greenhouse experiment examined hardwood seedlings’ response to two-phase flooding/drought treatments to measure the …

Social Dynamics, Network Structure, And Information Diffusion In Fish Shoals., Matthew Jerome Hasenjager

Electronic Theses and Dissertations

Animal populations are often highly structured, with individuals differing in terms of whom they interact with and how frequently they do so. The resulting pattern of relationships constitutes a population’s social network. In this dissertation, I examine how environmental variation can shape social networks and influence information flow within them. In Chapter I, I review the history of social network analysis in animal behavior research, and discuss recent insights generated by network approaches in behavioral ecology. I focus on the fields of: social learning, collective behavior, animal personalities, and cooperation. Animal network studies are often criticized for a lack of …

Improving Cardiac Cell Therapy: Role Of Hdac1 In Directing Cmc Cell Fate Decisions And Therapeutic Efficacy., John Zhao

Electronic Theses and Dissertations

Cardiac mesenchymal stromal cell (CMC) administration has improved cardiac function in pre-clinical animal models of heart failure. Specifically, both transdifferentiation and secretion of paracrine signaling molecules are potential mechanisms by which transplanted cells exert their cardiac reparative effects. The inhibition of histone deacetylases (HDACs) has been shown to enhance the cardiogenic differentiation capacity of various progenitor cell types. In the current study the consequences of HDAC1 inhibition on CMC in vitro differentiation, in vivo cardiac reparative capabilities, and in vitro cytokine secretion were investigated using either genetic depletion of HDAC1 or pharmacological inhibition with entinostat. CMCs depleted of HDAC1 exhibit …

Spinal Cord Injury Induced Cardiac Decline And The Limitations Of Exercise., Kathryn Michele Deveau

Electronic Theses and Dissertations

There is a general consensus that the level and the severity of spinal cord injury (SCI) impacts chronic cardiac abnormalities such that high thoracic and cervical SCI are the most severely affected. Furthermore, the extreme degree of immobility/inactivity experienced by the persons with SCI has implications on cardiac decline. Yet it has been difficult to separate the relative contributions of the decentralized autonomic nervous system (ANS) and the immediate and profound inactivity to chronic cardiac abnormalities. As such, this body of work sought to characterize a contusion SCI that results in persistent CV dysfunction. Echocardiography, Dobutamine stress echocardiography and pressure …

I. In The Time Of The Others : A Novel ; Ii. Out Of East Pakistan : Postcolonial Colony Bangladesh As A Case Study Of Postcolonial State And Postcolonial Nation-State From East Pakistan To Independence Through The Liberation War Of 1971 : A Critical Analysis., Nadeem Zaman

Electronic Theses and Dissertations

This dissertation is a combined creative and critical project consisting a novel and a theoretical component. The novel entitled In the Time of the Others is a fictional account set during the true event of the Bangladesh Liberation War of 1971. Using the war as a backdrop, the novel tells the story of one man trying to manage his family, marriage, and financial situation by returning to an inheritance he never claimed. The journey brings him from his home in southern East Pakistan (present-day Bangladesh) to the capital city Dhaka, to the home of his maternal uncle and aunt under …

From Damsel In Distress To Active Agent : Female Agency In Children's And Young Adult Fiction., Megan Sarah Mcdonough

Electronic Theses and Dissertations

This dissertation analyzes the different ways in which female characters in children’s and young adult fiction can claim agency. Using adaptation theory, feminist theory, and theories of agency and autonomy, this project examines how portrayals of female protagonists have changed to accept a multiplicity of strong females, and why we need these different kinds of characters within our culture. Working with the definition of agency as the choices one makes and the subsequent actions she takes, this dissertation examines how female characters from paradigm shifting texts claim agency. Each chapter uses a specific feminist lens to explore literary texts and …

Expression Of Zeb Factors In Oral Squamous Cell Carcinoma., Saira Ahmed

Electronic Theses and Dissertations

Objectives: The ZEB family of transcription factors (ZEB1 and ZEB2) have been demonstrated to play a role in metastatic progression of several cancers, and may also influence the initial transformation and generation of cancer stem cells. However, the expression pattern of ZEB proteins in the development of oral squamous cell carcinoma has not been investigated. The purpose of this study was to define changes in expression and subcellular localization of the ZEB family in both precancerous lesions and different grades of OSCC. Materials and Methods: Seventy-nine tongue biopsies were subjected to immunohistochemistry and immunofluorescence to determine the expression and subcellular …

The Interaction Of Homoserine Lactones And Paraoxonase 2 Modulates Cell Death Signaling And Cell Proliferation., Aaron Mackallan Neely

Electronic Theses and Dissertations

Pseudomonas aeruginosa produces N-(3-oxododecanoyl)-homoserine lactone (C12) as a quorum-sensing molecule that functions to facilitate bacteria-bacteria communication. C12 has also been reported to affect many aspects of human host cell physiology, including evoking cell death in various types of cells. However, the signaling pathway(s) leading to C12-triggerred cell death remains unclear. To clarify cell death signaling induced by C12, we examined mouse embryonic fibroblasts (MEFs) deficient in one or more caspases. Our data indicate that, unlike most apoptotic inducers, C12 evokes a novel form of apoptosis in cells, probably through the direct induction of mitochondrial membrane permeabilization. Importantly, C12 induces apoptosis …

A Tool To Evaluate Sustainability Of Pavement Maintenance Projects., Yibo Zhang

Electronic Theses and Dissertations

Pavement maintenance activities are widely regarded to be critical elements during pavement life cycle, different maintenance techniques have been used to restore or extend the service lives of pavements. However, the existing maintenance practices on pavement system have problems that impair the economic benefits, disturb the natural environment, and somewhat ignore the needs from stakeholders. To minimize these negative impacts, sustainability is an ideal way because it addresses the problems under Triple Bottom Line (TBL, refers to economy, environment, and society). Sustainability analysis within transportation or pavement industry has been studied in different countries, but the concept of sustainable pavement …

Don't Go Unless You Mean It : A Novel With Critical Afterword : Heteronormative Masculine Performance In Contemporary Fictions Of The Rural American South., Nathan N. Gower

Electronic Theses and Dissertations

This dissertation consists of a full-length novel for an adult audience as well as a substantial critical afterword to elucidate and complicate germane thematic concerns of the creative artifact. The novel, leaning on the rich traditions and many genre conventions of fictions of the American south, tells the story of an unlikely underground coal miner in Blue Banks, a fictional southern town set in the caverns and rolling hills of the western Kentucky coal fields. The narrative follows its fish-out-of-water protagonist, Cody Culver, a fledgling academic who thought he had escaped his fate as a third-generation coal miner when he …

Development And Evaluation Of A Biocompatible Electroactive Sensor For Continuous Blood Pressure Measurement., Scott D. Cambron

Electronic Theses and Dissertations

Piezo-active composites have been implemented for sensing and transduction for decades. The 0-3 ceramic/polymer composite is one of the most common composite types used for sensing applications, owing to their tailorable properties of the two-phase composition, consisting of a three-dimensionally connected polymer/rubber matrix (inactive phase) with a dispersion of isolated piezo-ceramic particles (active phase). This thesis describes a method to develop novel biocompatible perivascular band comprised of a two-phase piezo-active composite to be fabricated using simple manufacturing processes. Biomaterials such as tissue scaffolds comprised of silk fibroin (SF) and chitosan (CS), and biocompatible soft rubbers will be implemented as the …

Hard To See Through The Smoke : Remembering The 1912 Hillsville, Virginia Courthouse Shootout., Travis A. Rountree

Electronic Theses and Dissertations

This dissertation examines rhetorical rememberings of the 1912 Hillsville, Virginia courthouse shootout. It begins with an overview of the historical event, then through four chapters focuses on different rememberings that take up the event. Using Burke’s terministic screens, the study presents several lenses through which to view these rememberings. Chapter One presents the national and local newspaper constructions of the shootout in three terministic screens: the violent mountaineer, the gangster, and the uncolonized other. These three screens predate what is now the hillbilly image of the mountaineer. Chapter Two analyzes performative actions of the shootout. The ballads about the event …

Finding Exuberance For The Role Shelby In Baltimore., Danielle Smart

Electronic Theses and Dissertations

This thesis documents my discovery of exuberance as the key to a successful performance and reveals the challenges I faced in developing a character whose major actions in the play fall into the category of masking (i.e. hiding, avoiding, dodging). I define exuberance and discuss how I came to understand that sustaining exuberance requires a great deal of technical expertise specifically in acting, movement and voice in order to accommodate the demands of the character. I contend that exuberance also requires commitment and nurturing of the actor’s personal artistic vision. This thesis will discuss some of the difficulties I faced …