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Undesirable Forms, Maria Risner May 2018

Undesirable Forms, Maria Risner

Electronic Theses and Dissertations

The Master of Fine Arts exhibition, Undesirable Forms, presents a collection of paintings and sculptures that focus on the repulsion and discomfort a woman can experience within her mind and body. These works were exhibited at the Tipton gallery, in downtown Johnson City. The pieces included in this exhibition consist of encaustic paintings on panel, and sculptures created from plaster life-casts.

The ideas discussed in this paper are influenced by Julia Kristeva and Sigmund Freud’s work relating to the abject/grotesque female body. This paper also discusses inspiration from other artists, such as Natalie Frank and Helen Chadwick, who work …


A Role Of Vitamin B2 In Reducing Amyloid-Beta Toxicity In A Caenorhabditis Elegans Alzheimer’S Disease Model, Muhammad Tukur Ameen May 2018

A Role Of Vitamin B2 In Reducing Amyloid-Beta Toxicity In A Caenorhabditis Elegans Alzheimer’S Disease Model, Muhammad Tukur Ameen

Electronic Theses and Dissertations

Alzheimer’s disease (AD) is associated with amyloid-beta peptide deposition and loss of mitochondrial function. Using a transgenic C. elegans AD worm model expressing amyloid-beta in body wall muscle, we determined that supplementation with either of the forms of vitamin B2, flavin mononucleotide (FMN) or flavin adenine dinucleotide (FAD) protected against amyloid-beta mediated paralysis. FMN and FAD were then assayed to determine effects on ATP, oxygen consumption, and reactive oxygen species (ROS) with these compounds not significantly improving any of these mitochondrial bioenergetic functions. Knockdown of the daf-16/FOXO transcriptional regulator or the FAD synthase enzyme completely abrogated the …


The Combined Neuropharmacology And Toxicology Of Major 'Bath Salts' Constituents Mdpv, Mephedrone, And Methylone, Serena Allen May 2018

The Combined Neuropharmacology And Toxicology Of Major 'Bath Salts' Constituents Mdpv, Mephedrone, And Methylone, Serena Allen

Electronic Theses and Dissertations

The synthetic cathinones, 3,4- methylenedioxypyrovalerone (MDPV), 4-methylmethcathinone (mephedrone), and 3,4- methylenedioxymethcathinone (methylone), gained worldwide notoriety as the psychoactive components of ‘bath salts;’ a marketing term used to circumvent federal drug laws and permit their legal sale. Previous studies have shown that these drugs share pharmacological characteristics with cocaine and the amphetamines, however, descriptions of their neurotoxic properties are limited. Moreover, while forensic analysis has revealed that the most frequently abused bath salts ‘brands’ contain binary and ternary mixtures of MDPV, mephedrone, and methylone, the majority of preclinical research has focused on explicating the individual effects of these drugs. Therefore, the …


Diel Rhythmicity Found In Behavior But Not Biogenic Amine Levels In The Funnel-Web Spider Agelenopsis Pennsylvanica (Araneae, Agelenidae), Alexander E. Demarco May 2018

Diel Rhythmicity Found In Behavior But Not Biogenic Amine Levels In The Funnel-Web Spider Agelenopsis Pennsylvanica (Araneae, Agelenidae), Alexander E. Demarco

Electronic Theses and Dissertations

Quantifying individual differences in behavior and the extent that behavior is influenced by circadian control is of paramount importance in behavioral ecology. In addition, the proximate mechanisms underlying behavior are also critical in order to obtain a more complete picture of how behavior evolves. Biogenic amines (BAs) are simple nitrogenous compounds derived from amino acids and have been consistently and extensively linked to behavior. For this study, we analyzed temporal patterns of BAs in relation to the antipredator (boldness) and aggressive behavior in female Agelenopsis pennsylvanica, a funnel-web spider. Using HPLC-ED, we compared behavioral responses to temporal patterns of …


The Effect Of Alcohol Consumption On Adipokine Secretion, Ashley Degroat May 2018

The Effect Of Alcohol Consumption On Adipokine Secretion, Ashley Degroat

Electronic Theses and Dissertations

Alcoholic Fatty Liver Disease (AFLD) is caused by excessive alcohol consumption and is a leading cause of liver related mortalities, with currently no treatments available. The goal of this project was to establish the effect of alcohol consumption on adipose tissue-derived secreted factors, adiponectin and C1q TNF Related Proteins 1-3 (CTRP1-3). We propose that excessive alcohol consumption will reduce circulating levels of adiponectin and CTRPs 1-3. Mice were fed a Lieber-Decarli control or alcohol diet for 10-days with a gavage (NIAAA model) or 6-weeks with no gavage (chronic model). Serum and adipose tissue were collected and CTRPs 1-3 and adiponectin …


A Comparison Of Four Wetlands Assessment Procedures Relevant To The Alluvial Valleys Of East Texas, Amy Camp May 2018

A Comparison Of Four Wetlands Assessment Procedures Relevant To The Alluvial Valleys Of East Texas, Amy Camp

Electronic Theses and Dissertations

Currently several methods are used by public and private entities to assess wetland functional condition in support of the Section 404 Clean Water Act program. Four of these are: Forested Wetlands in Alluvial Valleys of the Coastal Plain of the Southeastern United States (SEHGM); A Regional Guidebook for Applying the Hydrogeomorphic Approach to the Functional Assessment of Forested Wetlands in Alluvial Valleys of East Texas (ETXHGM); Texas Rapid Assessment Method (TXRAM); and the Wildlife Habitat Appraisal Procedure (WHAP). Little previous research compares wetland functional assessment methods. Results of the SEHGM, ETXHGM, TXRAM, and WHAP methods were compared on five East …


The Preservation Of The Colonial Spanish Horse And Robert E. Brislawn, Sr., Gretchen L. Patterson Ms. Apr 2018

The Preservation Of The Colonial Spanish Horse And Robert E. Brislawn, Sr., Gretchen L. Patterson Ms.

Electronic Theses and Dissertations

The Preservation of the Colonial Spanish Horse and

Robert E. Brislawn, Sr.

In 1493, the Spanish horse importation to the Caribbean islands provided an important stimulus to American exploration as an agent of transportation. In the late nineteenth century, the necessity of horses declined with the advent of railroads and motorized vehicles. Reduction of Native American populations through wars, disease, and forfeiture of territory also contributed to less reliance on horses. Born in 1890, Robert E. Brislawn, Sr., a dedicated horseman, decided to collect and preserve the best Spanish-type horses that he could locate as links to western heritage. From …


Survey Of Rickettsia Spp. In Ticks In Nacogdoches County, Texas, Nathaniel Blakley Apr 2018

Survey Of Rickettsia Spp. In Ticks In Nacogdoches County, Texas, Nathaniel Blakley

Electronic Theses and Dissertations

Rickettsia parkeri is an obligate intracellular pathogenic bacterium that is commonly transmitted by the Gulf Coast tick, Amblyomma maculatum. Rickettsia parkeri is the causative agent of Rickettsia parkeri rickettsiosis, which is a disease characterized by nonspecific symptomology. Significant effort by numerous research groups focuses on determining the geographic distribution of potential vectors of this pathogen. The purpose of this study was to study A. maculatum populations in Nacogdoches County, Texas, for the presence of Rickettsia parkeri. Over a two-year period, 49 ticks were collected in Nacogdoches county and taxonomically identified. The DNA was extracted using DNeasy Blood and Tissue …


Entry-Level Physical Therapy Students' Knowledge And Attitudes Of Nutrition, Jordan D. Day Apr 2018

Entry-Level Physical Therapy Students' Knowledge And Attitudes Of Nutrition, Jordan D. Day

Electronic Theses and Dissertations

Like other allied health fields, physical therapy has incorporated health promotion as a part of their practice. A large and important component of health promotion is nutrition. Little research has been conducted on the knowledge and attitudes of nutrition in physical therapists and has only included small sample sizes. Therefore, the purpose of the present study was to expand upon the knowledge and attitudes of nutrition in physical therapy students. Subjects included doctoral physical therapy students from across the United States, ranging from first year to senior level. The present research was conducted online via a Qualtrics survey. Surveys, included …


Design And Development Of A Multifunctional Surgical Device For Ground And Space-Based Surgical Applications., Brooke Elaine Barrow Apr 2018

Design And Development Of A Multifunctional Surgical Device For Ground And Space-Based Surgical Applications., Brooke Elaine Barrow

Electronic Theses and Dissertations

With the possibility of longer ventures into space, NASA will face many new medical challenges. The ability to surgically treat trauma and other disorders in reduced gravity requires reliable wound access, containment, and visualization. In collaboration with Carnegie Mellon University, the University of Louisville is currently developing the AISS (Aqueous Immersion Surgical System) to increase efficiency and control of the operative field in space-based surgeries. Reliable wound access and containment is achieved by placing a transparent wound-isolation dome securely over the wound-site and pressurizing it with a saline solution. Leak-free trocars provide access ports for various surgical instruments. This system …


Gated Fences, Shaina Hawkins Apr 2018

Gated Fences, Shaina Hawkins

Electronic Theses and Dissertations

Gated Fences is set in 1941 America and revolves around the lives of two families: The Hendersons and the Rivers. This novel addresses discrimination that existed among acquaintances, neighbors, friends, and even family members due to the lingering effects of slavery. It tackles issues such as forbidden love, life, and death obstacles, and the harsh realities faced by those who lived in a “separate but equal” society. Readers will learn about the value of family, the importance of friendship, reasoning behind why the characters place their faith in God, and the hope that conquers even in the darkest hour.


Influence Of Stereotyping And Prejudices On Perceptions Of Superheroes, Emily A. Sanchez Mar 2018

Influence Of Stereotyping And Prejudices On Perceptions Of Superheroes, Emily A. Sanchez

Electronic Theses and Dissertations

Fictional superheroes possess characteristics that can be identified with on a human level (e.g., gender and race); however, their superhuman nature may lead them be evaluated as a distinct outgroup. The current study attempted to assess how racial and gender attitudes may affect perceptions of superheroes and how other participant characteristics may impact ratings. The current study found that gender and race influenced evaluations of perceived attributes of superhero drawings. Figures depicted with an unnatural skin tone were rated less favorably. Although, there were no differences between ratings of Black and White heroes, Black heroes were consistently rated higher, particularly …


Influence Of Stereotyping And Prejudices On Perceptions Of Superheroes, Emily A. Sanchez Mar 2018

Influence Of Stereotyping And Prejudices On Perceptions Of Superheroes, Emily A. Sanchez

Electronic Theses and Dissertations

Fictional superheroes possess characteristics that can be identified with on a human level (e.g., gender and race); however, their superhuman nature may lead them be evaluated as a distinct outgroup. The current study attempted to assess how racial and gender attitudes may affect perceptions of superheroes and how other participant characteristics may impact ratings. The current study found that gender and race influenced evaluations of perceived attributes of superhero drawings. Figures depicted with an unnatural skin tone were rated less favorably. Although, there were no differences between ratings of Black and White heroes, Black heroes were consistently rated higher, particularly …


F. Scott Fitzgerald As A "Hot Nietzschean": The Influence Of Friedrich Nietzsche's Philosophy In This Side Of Paradise, The Beautiful And Damned, And The Great Gatsby, Lindsey Carman Jan 2018

F. Scott Fitzgerald As A "Hot Nietzschean": The Influence Of Friedrich Nietzsche's Philosophy In This Side Of Paradise, The Beautiful And Damned, And The Great Gatsby, Lindsey Carman

Electronic Theses and Dissertations

Beginning in 1915, F. Scott Fitzgerald was exposed to the ideas of Friedrich Nietzsche under the guidance of mentors and from his personal reading lists. While reading Nietzsche, Fitzgerald's concern with the rise of cultural pessimism in 1920s America appeared in his fiction. Interestingly, both the philosopher and author explore the decline of Western culture in the twentieth century––a period of identity crises that affected America and Europe. This thesis investigates Fitzgerald's misreading of Nietzschean ideas that appears in his fiction to highlight the author's interest in explaining the cause of America's decline. In particular, this thesis appropriates a Nietzschean …


Development Of Low Frequency Electron Paramagnetic Resonance Methods And Instrumentation For Biological Applications, Laura A. Buchanan Jan 2018

Development Of Low Frequency Electron Paramagnetic Resonance Methods And Instrumentation For Biological Applications, Laura A. Buchanan

Electronic Theses and Dissertations

EPR is a powerful biophysical tool that can be used to measure tumor physiology. With the addition of magnetic field gradients, the spectral properties of paramagnetic species can be mapped. To facilitate EPR imaging, methods and instrumentation at frequencies between 250 MHz and 1 GHz were developed.

At low spin concentrations, the rapid scan background signal is often many times larger than the EPR signal of interest. To help remove the background contribution, a data acquisition procedure that takes advantage of a cross-loop resonator and bipolar power supplies was developed at 250 MHz. In this procedure, two scans are collected. …


Narrativizing Theory: The Role Of Ambiguity In Religious Aesthetics, Benjamin John Peters Jan 2018

Narrativizing Theory: The Role Of Ambiguity In Religious Aesthetics, Benjamin John Peters

Electronic Theses and Dissertations

This project expands S. Brent Plate's "invented religious aesthetics" by bringing it into conversation with Umberto Eco's theory of ambiguity. It articulates the space that ambiguity opens within the field of religious aesthetics when viewed as a liminal or interdisciplinary theory that neither privileges the starting points of transcendental aesthetics nor the "neo-arches" of theories of materiality. It hints at new ways of studying and describing religious worlds while also illustrating the porous borderlines between narrative and theory. It argues that a religious aesthetic rooted in ambiguity emphasizes both the provisionality of knowledge and the narrativization of reality.


Studying Facial Expression Recognition And Imitation Ability Of Children With Autism Spectrum Disorder In Interaction With A Social Robot, Farzaneh Askari Jan 2018

Studying Facial Expression Recognition And Imitation Ability Of Children With Autism Spectrum Disorder In Interaction With A Social Robot, Farzaneh Askari

Electronic Theses and Dissertations

Children with Autism Spectrum Disorder (ASD) experience limited abilities in recognizing non-verbal elements of social interactions such as facial expressions [1]. They also show deficiencies in imitating facial expressions in social situations. In this Master thesis, we focus on studying the ability of children with ASD in recognizing facial expressions and imitating the expressions using a rear-projected expressive humanoid robot, called Ryan. Recent studies show that social robots such as Ryan have great potential for autism therapy. We designed and developed three studies, first to evaluate the ability of children with ASD in recognizing facial expressions that are presented to …


Social Conflict On The Seas: Links Between Overfishing-Induced Marine Fish Stock Declines And Forced Labor Slavery, Jessica L. Sparks Jan 2018

Social Conflict On The Seas: Links Between Overfishing-Induced Marine Fish Stock Declines And Forced Labor Slavery, Jessica L. Sparks

Electronic Theses and Dissertations

Despite media attention detailing labor abuses in fisheries, social-ecological systems research has largely failed to consider whether fish stock declines could be contributing to increases in forced labor slavery. Empirical fisheries data suggests, though not a ubiquitous response to declining stocks, many vessels will fish longer, farther from shore, and deeper in waters to maintain yields. This effort intensification increases production costs, and Brashares et al. (2014), consistent with slavery theory, posited cheap and/or unpaid labor as an approach to offset increasing costs and continue harvesting fish species at a rate otherwise cost-prohibitive.

Using fuzzy cognitive mapping - a participatory, …


Fact Vs. Fiction: Uncovering The Experiences Of Homeless/Street Youths' Involvement In Survival Sex, Amber R. Mcdonald Jan 2018

Fact Vs. Fiction: Uncovering The Experiences Of Homeless/Street Youths' Involvement In Survival Sex, Amber R. Mcdonald

Electronic Theses and Dissertations

Youth involvement in trading/selling sex has become of concern for policy makers, scholars, and the general public in recent years (Cecchet & Thorburn, 2014; Hossain, et al, 2010; Pub L No. 106-386; Reid & Piquero, 2013; Sapiro, Johnson, Postmus, & Simmel, 2016; Shared Hope International, 2015). The behavior of trading/selling sex is defined dichotomously within the literature. One body of literature, employs the term "survival sex" when empirical work focuses on trading/selling sex among lesbian, gay, bisexual, transgender, or queer (LGBTQ) identified individuals or youth that are homeless (Greene, Ennett & Ringwalt,1999; Halcon & Lifson, 2004; Walls & Bell, 2011). …


Away From The End Of Motherhood: Sites Of Haunting In The Social Imaginary In Lemonade And The Handmaid's Tale, Julia Michele Fleming Jan 2018

Away From The End Of Motherhood: Sites Of Haunting In The Social Imaginary In Lemonade And The Handmaid's Tale, Julia Michele Fleming

Electronic Theses and Dissertations

This thesis analyzes the television series adaptation of The Handmaid's Tale, specifically the episode "A Woman's Place," and Beyoncé's Lemonade: A Visual Album. I argue that these cultural texts leverage representations of women's lived experiences to scrutinize contemporary American anxieties about motherhood and reproductive justice. Lemonade, a celebration of Black womanhood, presents a counterpoint to The Handmaid's Tale's preoccupation with white motherhood in way that speculates on the utopian potentials of a woman-centered society.

Using bell hooks' film analysis, Avery Gordon's "haunting," and Luce Irigaray's "mimicry," I examine two interconnected themes: feminist aesthetics and generational haunting. …


Patient-Specific Quantification Of The Relationship Between The Left Atrium Pressure And The Ostial Diameter Of The Left Atrial Appendage, Sky Tianqi Gao Jan 2018

Patient-Specific Quantification Of The Relationship Between The Left Atrium Pressure And The Ostial Diameter Of The Left Atrial Appendage, Sky Tianqi Gao

Electronic Theses and Dissertations

The left atrial appendage has been a historically understudied region of the heart until fairly recently with the new understanding of its role in the stroke pathway of patients with atrial fibrillation. The goal of this study is to take a look at the biomechanical behavior of the left atrium and left atrial appendage under normal physiological loading conditions using material properties taken from biaxial stretch tests. Several different options for material properties models were tested and biaxial stretch test data of cadaveric human tissue samples for the left atrium and appendage were fit to a Fung-type strain-energy function for …


Robust Distributed Stabilization Of Interconnected Multiagent Systems, Vahid Rezaei Jan 2018

Robust Distributed Stabilization Of Interconnected Multiagent Systems, Vahid Rezaei

Electronic Theses and Dissertations

Many large-scale systems can be modeled as groups of individual dynamics, e.g., multi-vehicle systems, as well as interconnected multiagent systems, power systems and biological networks as a few examples. Due to the high-dimension and complexity in configuration of these infrastructures, only a few internal variables of each agent might be measurable and the exact knowledge of the model might be unavailable for the control design purpose. The collective objectives may range from consensus to decoupling, stabilization, reference tracking, and global performance guarantees. Depending on the objectives, the designer may choose agent-level low-dimension or multiagent system-level high-dimension approaches to develop distributed …


Curiosity And Gifted Identification: A Mixed Methods Study, Cameron Hays Jan 2018

Curiosity And Gifted Identification: A Mixed Methods Study, Cameron Hays

Electronic Theses and Dissertations

This dissertation was designed to explore the link between epistemic curiosity and giftedness, in order to provide an additional potentially non-biased assessment to contribute to gifted identification "body of evidence" files for underrepresented and underserved populations. Students of color and students of lower socio-economic status are less likely to be placed into gifted and talented enrichment programs (Donovan & Cross, 2002). If a significant relationship is found between high curiosity and students who benefit from gifted education, psychometric instruments used for measuring curiosity could be invaluable in placing students in appropriate educational environments.

The study used a mixed methods design …


Effects Of Anthropogenic Noise On Mating Behavior And Fitness, Gabrielle A. Gurule-Small Jan 2018

Effects Of Anthropogenic Noise On Mating Behavior And Fitness, Gabrielle A. Gurule-Small

Electronic Theses and Dissertations

When environments change rapidly, adaptive phenotypic plasticity can ameliorate negative effects of environmental change on survival and reproduction. Recent evidence, however, suggests that plastic responses to human induced environmental change are often maladaptive or insufficient to overcome novel selection pressures. Anthropogenic noise is a ubiquitous and expanding disturbance with demonstrated effects on fitness-related traits of animals like stress responses, foraging, vigilance, and pairing success. Elucidating the lifetime fitness effects of noise has been challenging because long-lived vertebrate systems are typically studied in this context. In both chapters described herein, I reared field crickets, Teleogryllus oceanicus, in masking traffic noise, …


Developing An Affect-Aware Rear-Projected Robotic Agent, Ali Mollahosseini Jan 2018

Developing An Affect-Aware Rear-Projected Robotic Agent, Ali Mollahosseini

Electronic Theses and Dissertations

Social (or Sociable) robots are designed to interact with people in a natural and interpersonal manner. They are becoming an integrated part of our daily lives and have achieved positive outcomes in several applications such as education, health care, quality of life, entertainment, etc. Despite significant progress towards the development of realistic social robotic agents, a number of problems remain to be solved. First, current social robots either lack enough ability to have deep social interaction with human, or they are very expensive to build and maintain. Second, current social robots have yet to reach the full emotional and social …


An Archaeological Exploration Of Agriculture, Trade, And Indigenous Relationships At A Seventeenth-Century New England Site, Jasmine Coreen Saxon Jan 2018

An Archaeological Exploration Of Agriculture, Trade, And Indigenous Relationships At A Seventeenth-Century New England Site, Jasmine Coreen Saxon

Electronic Theses and Dissertations

A multi-method approach including ground-penetrating radar, magnetometry, historical research, excavations, and artifact analyses was used to gather data at a 17th century archaeological site in South Glastonbury, Connecticut. Interpretation of these data provided evidence that the Europeans who occupied this site were involved in a variety of activities such as agriculture, trade, and developing Indigenous relationships. These activities included cultivating an agricultural surplus instead of relying on subsistence farming, access to trading networks that extended throughout the Colonies and into Europe, and cohabitation with the Indigenous peoples in the area. This research led to an examination of various historical narratives …


Looking Through The Trees: An Anthropologist, A Museum, And The Sasquatch, Carissa Kepner Jan 2018

Looking Through The Trees: An Anthropologist, A Museum, And The Sasquatch, Carissa Kepner

Electronic Theses and Dissertations

The Sasquatch is incredibly popular in American society. This project explores the impact of the Sasquatch phenomenon on those that live in and visit Bailey, Colorado. It focuses on how the Sasquatch Outpost museum contributes to this impact, especially through outdoor activities, visiting the museum, visiting the Outpost general store, and the sharing of sighting stories. This work takes an in-depth look at the concept of the amateur museum, or micromuseum, and how it can contribute to the larger museum world, particularly through the ideas of Outsider Art and serious leisure. By evaluating the processes, exhibits, and procedures of the …


Whither The Gender Of Get Out: A Critique Of The Cinematic (Im)Possibilities Of The Black Political Imagination, Daelena Tinnin Jan 2018

Whither The Gender Of Get Out: A Critique Of The Cinematic (Im)Possibilities Of The Black Political Imagination, Daelena Tinnin

Electronic Theses and Dissertations

This thesis investigates the entanglements of spatialized racial-sexual violence, conceptualizations of black female subjectivity, questions of the limitations and excesses of media representations and the socioeconomic, cultural and spatiotemptoral relations that make black images visible and (im) possible as they are situated in the cinematic black political imagination. Through a materialist media analysis of the 2017 film Get Out, I argue that the film and its articulation of the afterlife of slavery fails to account for gender by tangentially engaging black women in its dissection of race and racism. I contend that black women are the absent presence in …


Origins Of Animal Cell Adhesion, Jennyfer Mora Mitchell Jan 2018

Origins Of Animal Cell Adhesion, Jennyfer Mora Mitchell

Electronic Theses and Dissertations

A fundamental requirement for multicellularity is cell adhesion. This includes mechanisms by which cells adhere to each other and to their secreted extracellular matrix (ECM). Two well characterized adhesion complexes in animals include: 1) adherens junctions (AJs), which are involved in cell-cell adhesion and composed of cadherin receptors, p120-, α- and β-catenin, and 2) focal adhesions (FAs), which are involved in cell-ECM adhesion and include proteins such as integrins, vinculin, paxillin, talin and focal adhesion kinase (FAK). The molecular components of AJs are widely conserved in animals, and largely absent in non-animals. In contrast, the molecular components of FAs have …


Improving The Detection Limit Of Tau Aggregates For Use With Biological Samples, Emily Rickman Hager Jan 2018

Improving The Detection Limit Of Tau Aggregates For Use With Biological Samples, Emily Rickman Hager

Electronic Theses and Dissertations

The protein Tau is found in neurofibrillary tangles in Alzheimer's disease and over 20 other neurodegenerative diseases. An assay has been developed to detect minute amounts of fibrils from human brain tissue. This assay subjects brain tissue extract and recombinant Tau to several rounds of sonication and incubation. Incubation allows recombinant Tau to add itself to the ends of the existing fibrils in brain tissue extract. Sonication breaks the existing fibrils in the brain tissue extract offering more ends for Tau to add onto. Cycles of sonication and incubation have been shown to allow for amplification of Tau fibrils from …