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Therapeutic Immunoglobulin G Surface Mobility Promotes Antibody-Dependent Cellular Phagocytosis By Arp2/3 And Syk Mediated Rearrangement Of Fcγ Receptors, Seongwan Jo
Electronic Theses and Dissertations
Therapeutic antibodies are rapidly growing class of drugs for treating cancers and immunological disorders by targeting specific antigens or blocking certain pathway to inhibit the tumor growth. In cancer immunotherapy, it was found that Fcγ receptor (FcγR) in immune cells play an important role in antibody dependent cell toxicity against tumor cells. Therefore, understanding FcγR activation mechanism, which requires ligation with immunoglobulin (IgG), is crucial to enhance potency of therapeutic antibodies. One crucial immune responses triggered upon FcγR ligation with IgG is phagocytosis which indicates clearing targets by engulfment. It is known that FcγRs form microclusters which gets rearranged during …
Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez
Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez
Electronic Theses and Dissertations
This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …
Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar
Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar
Electronic Theses and Dissertations
Phospholipids (PLs) found in milk are of great interest due to their health and nutritional benefits associated with their consumption. By-products generated during the manufacture of dairy products represent a source of PLs with potential for value-added opportunities. Currently, the extraction of PLs from by-products results in overall lowefficiencies, and it involves subsequent solvent separation and lipid recovery. This dissertation studied the extraction of milk PLs from by-product streams with a tertiary anime (N, N-dimethyl cyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent. This type of solvents can be reversibly switched between a hydrophobic and hydrophilic form in the presence or …
College Students Conceptions Of Particulate Nature Of Matter And The Impact On Research Instrumentation, Bridget Klutse
College Students Conceptions Of Particulate Nature Of Matter And The Impact On Research Instrumentation, Bridget Klutse
Electronic Theses and Dissertations
The main objective of this research was to explore the understanding and conceptions of Particulate Nature of Matter (PNM) with Chem 115 L students as the participants. Conceptions were defined specifically as beliefs and alternative beliefs about topics. The research also assessed the impact of analytical instrumentation in the chemistry laboratory on learning chemistry concepts. Eight questions (8) with multiple choice answers were administered to 10 students at the beginning and after the Fall 2018 semester via selective/purposeful sampling. Data were collected using surveys (pre- and post-surveys) and interviews (pre- and postinterviews), then analyzed quantitatively and qualitatively. The eight questions …
Implications Of Broadleaf, Grass, And A Blend Of Broadleaf And Grass Cover Crops On Soil Health And Corn Production In South Dakota, Hunter Bielenberg
Implications Of Broadleaf, Grass, And A Blend Of Broadleaf And Grass Cover Crops On Soil Health And Corn Production In South Dakota, Hunter Bielenberg
Electronic Theses and Dissertations
Cover crops have recently gained attention in the U.S. Midwest because of their potential to increase soil organic matter and protect overall soil health. This study was conducted to determine the effects of different cover crop mixtures on soil health measurements and corn grain yield at increasing nitrogen (N) rates. Cover crops were planted in the fall after small grains harvest as a dominantly grass mixture, dominantly broadleaf mixture, or a 50/50 grass and broadleaf mixture with a no cover crop control. Soil and cover crop biomass samples were collected in the fall before winter cold termination and in the …
Soil Organic Carbon, Hydro-Physical Properties, Pore Structure And Greenhouse Gas Fluxes Under Integrated Crop-Livestock System, Jashanjeet Kaur Dhaliwal
Soil Organic Carbon, Hydro-Physical Properties, Pore Structure And Greenhouse Gas Fluxes Under Integrated Crop-Livestock System, Jashanjeet Kaur Dhaliwal
Electronic Theses and Dissertations
Integrated crop-livestock system (ICLS) can improve soil organic carbon (SOC), enhance soil hydro-physical properties, and mitigate greenhouse gas (GHG) emissions. The objectives of this study were to: (i) investigate the influence of grazing, cover crop (CC), and a combination of grazing and CC on labile soil carbon (C) and nitrogen (N) fractions and soil enzyme activity, (ii) assess the impacts of short-term (3-4 years) and long-term (>30 years) ICLS on SOC, bulk density, structural quality and water infiltration, (iii) evaluate soil pore structural properties such as porosity, number of pores, pore size, pore shape, fractal dimension, anisotropy and tortuosity …
Impact Of Carcass Chilling System On Carcass Characteristics And Biochemical Changes In Beef Carcasses, Lydia Margaret Hite
Impact Of Carcass Chilling System On Carcass Characteristics And Biochemical Changes In Beef Carcasses, Lydia Margaret Hite
Electronic Theses and Dissertations
Effective and efficient chilling of beef carcasses will directly influence meat quality and consumer acceptance. Over the past century the United States’ meat industry has evolved, and spray chilling system have become common practice utilized by the beef industry. However, many of the chilling systems presently in use were designed to chill carcasses averaging lighter than the average beef hot carcass weight of today. Beef hot carcass weight has increased 68 kilograms from 1991 to 2019. The increase in beef hot carcass weight could result in longer chilling periods and present possible challenges for chilling systems used by the beef …
The Influence Of Carcass Weight On Beef Primal Temperature, Tenderness, And Palatability, Samantha Egolf
The Influence Of Carcass Weight On Beef Primal Temperature, Tenderness, And Palatability, Samantha Egolf
Electronic Theses and Dissertations
The United States beef industry has experienced increasing hot carcass weights (HCW) and improvements to quality grade the last three decades. Postmortem chilling of beef carcasses is critical to overall product quality. Carcass weight and composition may influence carcass temperatures during postmortem chilling. The objective of this research was to evaluate the effects of HCW and quality grade during postmortem chilling and on product quality. A-maturity carcasses were selected by weight [light = 296–341 kg; middle = 386–432 kg; heavy = 466–523 kg]. A 20 cm data logger was placed into the chuck and round and a 10 cm data …
Exploration Of Host Health Benefits By A Defined Consortium Of Butyrate-Producing Human Gut Bacteria In Gnotobiotic Mouse Model, Linto Antony
Electronic Theses and Dissertations
Aberrant gut microbiota composition is found to be associated with several human diseases such as inflammatory bowel diseases (IBD). Reduction in butyrate producing bacteria is one of the characteristic features of such dysbiotic bacterial community in the gut. Modulation of gut microbiota to bring the dysbiotic state back to normal healthy state is a promising therapeutic strategy to cure several diseases like recurrent Clostridium difficile infection (rCDI) and IBD where traditional therapies using pharmacological substances fails to make a difference. Restoration of butyrate producers is found to be an effective method of such gut microbiota modulation. The potential to produce …
Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan
Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan
Electronic Theses and Dissertations
This study aimed to evaluate the transcriptomic profile of both corium tissue from lactating dairy cows experiencing sole ulcers and skin lesions from lactating dairy cows diagnosed with digital dermatitis (DD). Hoof biopsies were performed in multiparous Holstein dairy cows selected based on their condition as clinically healthy or diagnosed with sole ulcers (n=7/group). Similarly, skin biopsies were taken from the center of active (M2/M4.1) DD lesions or non-active (M0/M4/M1) from multiparous Holstein dairy cows (n = 7/group) to assess the impact of DD on the skin transcriptome via RNA-seq analysis. All RNA samples were sequenced using Illumina, NovaSeq S4 …
Design, Synthesis, And Evaluation Of Glutathione Derivatives As Brain Targeting Agents, Yue Huang
Design, Synthesis, And Evaluation Of Glutathione Derivatives As Brain Targeting Agents, Yue Huang
Electronic Theses and Dissertations
Treatment of central nervous system (CNS) diseases, such as Alzheimer’s disease, Parkinson’s diseases, and brain tumors, is still a challenge due to the existence of the blood-brain barrier (BBB). The BBB as a membrane barrier that separates systemic blood circulation from the CNS is structurally formed by endothelial cells, astrocyte, pericyte and neurons and is one of the crucial protection mechanisms for the CNS. The BBB protects the brain by preventing foreign compounds (non-endogenous compounds) from entering the brain and helps maintain brain homeostasis. In addition, the presence of drug efflux pumps (i.e., P-glycoproteins, multidrug-resistance proteins) in the BBB helps …
Evaluating Impacts Of Tryptophan And Branched Chain Amino Acids In Swine Diets Containing Corn Based Dried Distillers Grains On The Growth Performance And Carcass Characteristics Of Grow-Finish Pigs, David Alan Clizer
Electronic Theses and Dissertations
A total of four studies were conducted in an effort to determine the impact of Trp and the BCAA in swine diets containing DDGS or HPDDG on the growth performance and carcass characteristics of the growing and finishing pig. The first study utilized 1,170 pigs (PIC 800 x PIC, initial BW 38.6 kg) in a 98-d grow-finish study to determine the performance response of pigs fed increasing levels of SID Trp:Lys in swine diets containing 40% DDGS. Treatments consisted of diets containing 40% DDGS with a SID Trp:Lys ratios of 15, 18, 21, or 24% or a corn-SBM diet for …
Electrical Sensing In Non-Excitable Cells To Promote Galvanotaxis And Tissue Survival, Anyesha Sarkar
Electrical Sensing In Non-Excitable Cells To Promote Galvanotaxis And Tissue Survival, Anyesha Sarkar
Electronic Theses and Dissertations
Weak DC fields have been shown to induce polarity, cell migration and cell proliferation in 2D cultures in vitro. To understand the mechanism by which non-excitable cells sense such weak EFs, we have investigated the mechanism of cathode-directed water flow (electro-osmosis) in the boundary layer of cells by reducing it with neutral, viscous polymers. Our results indicate that low molecular weight polymers decrease cathodal migration and promote anodal migration in a concentration dependent manner. High molecular weight polymers do not affect directionality and can be explained using porosity and hydraulic permeability between the polymers. These results provide the first evidence …
The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus
The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus
Electronic Theses and Dissertations
Nitrogen(N) is one of the most important plant nutrients for plant growth and yield, however, its abundance in the soil is not sufficient for profitable crop production. The use of chemical fertilizers helps address soil N deficiency in agriculture. However, due to the environmental pollution resulting from excessive use of fertilizers, alternative forms of N for agriculture are a necessity. Leguminous plants such as soybean (Glycine max) form a symbiotic association with N-fixing rhizobia to meet their N demands. Legume-rhizobia symbiosis results in the formation of unique structures called nodules where rhizobia convert atmospheric nitrogen into plant usable forms, thus …
The Interactions Of Change In Nutrition Prior To And After Artificial Insemination On Plasma Metabolites, Steroid Hormones, And Uterine Histotroph In Beef Heifers, Taylor N. Andrews
The Interactions Of Change In Nutrition Prior To And After Artificial Insemination On Plasma Metabolites, Steroid Hormones, And Uterine Histotroph In Beef Heifers, Taylor N. Andrews
Electronic Theses and Dissertations
The management of heifers can have lifetime effects on their reproductive success and efficiency. Nutrition is one of the management factors that can influence steroid hormone production, oocyte development, embryo development, and uterine histotroph, thus affecting heifers’ reproductive efficiency. Therefore, the objectives of the following studies were to evaluate the impact of nutritional changes prior to and after Artificial Insemination (AI) on plasma cholesterol concentrations, and uterine mineral concentrations (Chapter 2) and the impact of nutritional change after AI on uterine mineral concentrations, steroid hormone production, and circulating metabolites (Chapter 3). In experiment 1 (Chapter 2), beef heifers (n = …
Interrogating The Socio-Ethical Dilemmas Of Precision Agriculture Technologies, Ayorinde Ogunyiola
Interrogating The Socio-Ethical Dilemmas Of Precision Agriculture Technologies, Ayorinde Ogunyiola
Electronic Theses and Dissertations
Farming has moved into a digital age where data and information are available to farmers to make informed agronomic and financial on-farm decisions. The development of precision agriculture (PA), such as big data technologies and machine learning algorithms is transforming the agricultural food production system in diverse ways, economically, environmentally, and socially. Despite benefits afforded by PA to agricultural productivity and environmental sustainability, these technologies can raise unintended societal challenges that can also limit their adoption among farmers. PA is changing how farming is done, reconstructing farmers’ social identities, and influencing relationships between farmers, agronomists, and technology developers. This dissertation …
Rational Design Of Advanced Functional Materials For Electrochemical Devices, Shun Lu
Rational Design Of Advanced Functional Materials For Electrochemical Devices, Shun Lu
Electronic Theses and Dissertations
In recent years, there has been a fast-growing trend in developing urea (CO(NH2)2) as a substitute H2 carrier in energy conversion due to its high energy density, nontoxicity, stability, and nonflammability. Urea, a byproduct in the metabolism of proteins and a frequent contaminant in wastewater, is an abundant compound that has demonstrated favorable characteristics as a hydrogen-rich fuel source with 6.7 wt % gravimetric hydrogen content. Also, there is 2-2.5 wt % urea from mammal urine; therefore, 0.5 million ton of additional fuels will be produced per year just from human urine (240 million ton each year). Electrochemical oxidation has …
Biomechanical Investigation Of Plant Vascular Tissue For Bio-Inspired Design And Flexible Composites, Mukesh Roy
Biomechanical Investigation Of Plant Vascular Tissue For Bio-Inspired Design And Flexible Composites, Mukesh Roy
Electronic Theses and Dissertations
Since the inception of evolution, nature has continuously optimized all the natural materials. These optimizations are so efficient that scientists and engineers have started taking inspiration from nature. Such inspiration-based solutions provide sustainable development of new materials with enhanced structural properties and functionalities (such as stiffness, stability, toughness, self-healing etc.). This variety of properties and functionalities is mainly due to the hierarchical organization of such components. Abstraction of the hidden cues of the hierarchical organization, principles and underlying structure-function relations are some of the critical approaches of bioinspired materials design. Inspiration from animals and plants has helped develop the design …
An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler
An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler
Electronic Theses and Dissertations
Within the last century, bighorn sheep (Ovis canadensis) in the badlands ecosystem of western South Dakota have been subjected to complete extirpation, reintroduction, disease die-offs, genetic bottlenecking, and population augmentation. Subsequently, the population in Badlands National Park (BNP) appears to have recovered, but it was unknown to what degree past events had influenced the population. From 2017-2019, we conducted research on 5 subherds within 2 management units in BNP to 1) survey for the presence of respiratory pathogens and estimate the prevalence of other potentially infectious diseases; 2) assess adult and lamb survival and cause-specific mortality; 3) estimate population size …
Improving Nutritional Properties And Extractability Of Pea Proteins For Human Consumption Via Fungal Bioprocessing, Camille Massmann
Improving Nutritional Properties And Extractability Of Pea Proteins For Human Consumption Via Fungal Bioprocessing, Camille Massmann
Electronic Theses and Dissertations
Yellow peas are a rich source of protein and phytochemicals. Peas are compatible with many diets restricted by sensitivities, allergies, or personal choice. As a result, peas are gaining popularity in food markets, despite the challenges they present. The biggest challenges for pea proteins in food markets are high content of fiber, starch, and saponins. These components cause processing challenges and create undesirable textures and bitter flavors. Pea proteins are typically extracted with alkaline extraction or air classification methods which result in 80% and 50% protein products respectively. With low protein purity, these products are not competitive with other, more …
Encroachment: College Athletes’ Experiences With Identity Development As Affected By Media Representations Of Social Justice Demonstrations, Madison Remi Vanwalleghen
Encroachment: College Athletes’ Experiences With Identity Development As Affected By Media Representations Of Social Justice Demonstrations, Madison Remi Vanwalleghen
Electronic Theses and Dissertations
This study was conducted to understand how communication and identity are experienced by Black university athletes in asking about their experiences and how they gauge mainstream media portrayals of Black NFL athletes making verbal or physical statements about or in protest of social injustice, racism, and inequality in America. I interviewed five university athletes identifying as Black/African American or Mixed- Race using media representations of Black NFL athletes’ protests since the NFL has dominated mainstream media headlines regarding recent athletes’ protests, especially in connection to the Black Lives Matter social movement. I also used several theories to interpret the data …
The Effects Of Weather, Body Weight, And Feed Intake On Total And Liquid Water Intake In Beef Cattle, Zachary Mcdaniel
The Effects Of Weather, Body Weight, And Feed Intake On Total And Liquid Water Intake In Beef Cattle, Zachary Mcdaniel
Electronic Theses and Dissertations
Two completely randomized design feedlot experiments were conducted over the course of a year. Both experiments were performed in order to determine the effects of weather, body weight (BW), and feed intake on total daily water intake (TDWI) and liquid daily water intake (LDWI) in beef steers. Experiment 1 utilized Angus weaned steers (n = 27; initial BW = 273 + 21.3 kg). Experiment 2 utilized yearling Bos taurus steers (n = 26; initial BW = 421 + 16.2 kg). Individual feed and water intake were collected using the Insentec (Insentec RIC, Hokofarm, Marknesse, Netherlands) automated feeding and water system. …
Cortisol In Hair As A Measure Of Chronic Stress During Sow Gestation And The Pattern Of Cortisol In Blood During Parturition In Sows, Talia Everding
Cortisol In Hair As A Measure Of Chronic Stress During Sow Gestation And The Pattern Of Cortisol In Blood During Parturition In Sows, Talia Everding
Electronic Theses and Dissertations
Cortisol is known as the stress hormone, as it influences many metabolic processes to maintain glucose homeostasis during stressful experiences, including physical and psychological stress. It can be detected in biological matrices such as blood and hair and is released rapidly during sudden stressors and continuously during longterm stress. Blood cortisol fluctuates rapidly in response to acute stressors like pain, exertion, and fear; in hair cortisol accumulates steadily over the period of hair growth and may be useful for detecting chronically elevated cortisol resulting from long-term stress. The objective of this research was to, 1) determine the influence of a …
U.S. Consumers' Acceptance And Willingness To Pay For Genetically Modified And Genome-Edited Foods, Bindu Paudel
U.S. Consumers' Acceptance And Willingness To Pay For Genetically Modified And Genome-Edited Foods, Bindu Paudel
Electronic Theses and Dissertations
Even though genetically modified (GM) crops have a lot of economic potential for producers and consumers, they have not been widely accepted due to increasing concerns related to food safety, human, animal, and environmental health. Unlike GM technology, genome-editing technology does not involve the transfer of genetic materials. It is simpler, cheaper, more precise, and faster relative to GM technology and has immense potential for incorporating producer and consumer traits. Genome-editing technology, as a novel process, not has been explored much from the consumer’s side. This study uses a nationally representative survey of 1,573 U.S. consumers to examine their acceptance …
Isolation Of Marker Compounds From Copaiba Essential Oil And Mimosa Pigra For Quality Evaluation Of Dietary Supplements, Mohammed Hawwal
Isolation Of Marker Compounds From Copaiba Essential Oil And Mimosa Pigra For Quality Evaluation Of Dietary Supplements, Mohammed Hawwal
Electronic Theses and Dissertations
Natural products have been used as a primary means of treating illness since ancient times, and they remain an important pool of U.S. FDA approved drugs. Approximately 33% of all current pharmaceutical medications discovered between 1981 and 2014 are either natural products or derived from natural products (Newman and Cragg 2016). The process of isolating specialized metabolites from medicinal plants has led to the development of particularly important medicines worldwide (Rakotomalala, Agard et al. 2013) such as Taxol® from Taxus brevifolia Nutt. (Taxaceae) and salicylic acid from the bark of Salix spp. This research is composed of five chapters; the …
Studying Morphological, Physiological, And Molecular Regulation Of Stomatal Conductance And Its Relationship To Water Use Efficiency In Alfalfa, Surbhi Gupta
Electronic Theses and Dissertations
Alfalfa (Medicago sativa), being a leguminous, highly fibrous, and proteinaceous plant has always been one of the top choices for the forage production but has high cost of irrigation in many dry and warm areas such as California and Arizona. Thus, a reduction of irrigation by using the higher water use efficiency (WUE) varieties can help the growers in reducing the cost and is critical for sustainable agriculture production. WUE is closely related to water loss through transpiring stomata. A study in our lab reported that Riverside (RS) an alfalfa genotype that has naturized in the national grassland in South …
Long Term Evaluation Of E. Coli Removal From Stormwater Using Recycled Steel Byproducts, Brenden Olevson
Long Term Evaluation Of E. Coli Removal From Stormwater Using Recycled Steel Byproducts, Brenden Olevson
Electronic Theses and Dissertations
Stormwater runoff often carries a variety of pollutants and pathogens that can endanger public health and wildlife. One contaminant of concern often highlighted in stormwater quality is Escherichia Coli (E.Coli) because its presence indicates microbial contamination in water. Different types of media filtration are being studied to reduce contaminant concentrations in stormwater. In this study, steel byproducts were examined as a potential material to use in media filtration to remove E. Coli and other contaminants from stormwater. Batch scale studies were conducted in laboratory to evaluate the E. Coli removal potential of steel chips and steel slag over a 24-hour …
Novel Acetylcholine Chloride Based Water Immiscible Deep Eutectic Solvent: Formulation, Characterization And Application In Drug Solubilization, Shiksha Subedi
Novel Acetylcholine Chloride Based Water Immiscible Deep Eutectic Solvent: Formulation, Characterization And Application In Drug Solubilization, Shiksha Subedi
Electronic Theses and Dissertations
Deep eutectic solvents (DESs) are the emerging class of new and inexpensive solvents composed of two or three safe components capable of self-association through hydrogen bond interaction. The depression in the melting point characterizes DESs compared to those of the individual components. These solvents have properties like the traditionally used ionic liquids (ILs), but they can offset the significant drawbacks of ILs, such as biodegradability, toxicity, and complex synthesis. Due to these remarkable advantages, the research in DESs is increasing exponentially. However, current deep eutectic solvents still have limitations to apply to the real chemical industries due to hydrophilicity and …
Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam
Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam
Electronic Theses and Dissertations
BVDV is a major pathogen of ruminants that causes immune dysfunction and lead to secondary bacterial infection. The underlying mechanism of this dysfunction is not well understood. Based on previous results, we believe that macrophages play a central role in BVDV-associated immune dysfunction. Previously, we have shown that the infected monocyte-derived macrophage (MDM) supernatant of the high virulent strains of BVDV significantly induced lymphocyte apoptosis. In the current study, we performed experiments to identify the apoptotic factors in the infected MDM supernatants in vitro. Also, we have extended our investigation to include testing the impact of infected macrophages on neutrophil …
Influence Of Preharvest Management Strategies In Beef Cattle On Growth Performance, Carcass Characteristics, And Meat Quality, Erin Gubbels
Influence Of Preharvest Management Strategies In Beef Cattle On Growth Performance, Carcass Characteristics, And Meat Quality, Erin Gubbels
Electronic Theses and Dissertations
The overall goal of this thesis project was to better understand the influence of preharvest management strategies at critical growth and development periods on beef performance and meat quality. This was accomplished through two primary objectives: 1) to investigate the effects of maternal prepartum dietary energy source during mid- and late-gestation on growth performance, carcass composition, and meat quality of offspring and 2) to compare the influence of two low stress weaning methods with conventional weaning on post-weaning performance and carcass characteristics of steers. For objective 1, Angus-based cows from two sources (n = 129 from South Dakota State University, …