Digital Commons Network^™

Computational Frameworks For Microrna Functional Analysis Of Inter-Kingdom And Indirect Targeting., Mohammed Sayed

Electronic Theses and Dissertations

Genes are DNA sequences that encode the information needed to synthesize molecules necessary for the function of the cell. Some genes are called protein-coding genes because they have the code required to manufacture proteins. The expression of a certain gene means its product (protein) is produced. Although some genes are not protein-coding, they regulate the gene expression of other protein-coding genes. Of these, microRNAs (miRNAs) are small RNA molecules that inhibit the expression of other genes by binding to their mRNA transcripts. miRNAs have been shown to be linked to several biological processes like development and diseases like cancer. Recently, …

Major League Baseball Fans’ Climate Change Attitudes And Willingness To Adapt: Climate Vulnerability Vs. America’S Pastime., Jessica R. Murfree

Electronic Theses and Dissertations

Climate change threatens the ability to enjoy sport around the world, including in the United States. While the scientific community reached consensus regarding the presence and severity of climate change near the turn of the twenty-first century, that same agreement has not been met across the American general public. Major League Baseball (MLB) is particularly vulnerable to climate change in the U.S. due to its season duration, geographic footprint, and largely outdoor nature. Therefore, the purpose of this study was to investigate relationships between U.S.-based MLB fans’ sport identification and their climate change attitudes, perceptions of climate change risk, and …

Formula, Moment, And Ips In Stockhausen's Evas Erstgeburt., Jonathon Pan

Electronic Theses and Dissertations

This analysis covers the form in Stockhausen’s Evas Erstgeburt, the first act from his Monday opera, Montag aus Licht. It discusses the presence of Stockhausen’s musical formula within this act and observes that his formula is integrated at several structural levels. This formula is a passage of music that determines the pitches, rhythms, and structure of all the music of Stockhausen’s Licht cycle. This formula is a three-voice schema, and these three voices are named Michael, Eve, and Lucifer. While the formula creates landmarks in the music, which indicate towards form, a formula analysis is limited in describing the direction …

Vitamin B12 Deficiency Does Not Stimulate Amyloid-Beta Toxicity In A Ceanorhabditis Elegans Model Of Alzheimer’S Disease, Opeyemi F. Showemimo

Electronic Theses and Dissertations

Alzheimer’s disease (AD) is symptomized by amyloid-beta plaques in the brain and accounts for more than 65 percent of dementia cases. Vitamin B12 (cobalamin) deficiency can result in similar cognitive impairment and roughly 15% of the elderly are vitamin B12 deficient. Vitamin B12 deficiency results in the accumulation of toxic methylmalonic acid and homocysteine. Hyperhomocysteinemia is a strong risk factor for AD. To test if vitamin B12 deficiency stimulates amyloid-beta toxicity, Caenorhabditis elegans expressing amyloid-beta in muscle were fed either vitamin B12-deficient OP50-1 or vitamin B12-rich HT115(DE3) E. coli bacteria. Increased amyloid-beta toxicity was found in worms fed the …

Improving Student Success And Engagement In Virtual And Hybrid Classrooms., Sydney Fogo

Electronic Theses and Dissertations

The following thesis examines virtual classes from the University of Louisville in the Fall 2020 semester to discover points of weakness relative to keeping students engaged in online courses. Three surveys were taken by a select group of participants over the course of the fall semester in order to collect student opinion on specific classes. Interviews were held with a subset of students from the group of participants and also with professors who taught the study’s participants in the fall of 2020. Their responses and conversations were used to determine possible areas of improvement for virtual classes. This information was …

Covid-19 Serological Diagnostic Development Using A Sars-Cov-2 Rbd Foldon Fusion, Joey Emery Breckenridge Jr.

Electronic Theses and Dissertations

Serological tests are conducted to assess humoral response against viral protein antigens, to assess viral exposure and protection from pathogens. The rapid development and modularity of serological assays have proven critical to managing the current SARS-CoV-2 pandemic. The receptor binding domain of SARS-CoV-2 is within the trimeric Spike protein and serves as a highly immunogenic target for potentially neutralizing antibodies. Current receptor binding domain serological assays use recombinant monomers or dimers of the receptor binding domain. The receptor binding domain is presented to the immune system natively in the context of the Spike protein trimer. Therefore, a recombinant trimeric receptor …

Skin Deep: Body Modification And Agentic Identities Among Women With Skin Conditions, Christopher Walonski

Electronic Theses and Dissertations

This study explores processes of identity construction among women who have skin conditions and body modifications. Analyzing seven semi-structured qualitative interviews, the author examines how individuals affected by skin conditions employ body modification practices to organize their identities and promote feelings of agency across both personal and social domains. Engaging a Bakhtinian dialogic lens, the author argues that body modification may operate as a de-stigmatization strategy that supports individuals with skin conditions in cultivating a sense of self-determination and bodily sovereignty. Shaped by grounded theory, this study’s findings trace relationships between body modification and the development of agentic identities among …

The Mouse Sees No Color: An Examination Of The Disney Corporation’S Recent Depictions Of Race In American History, Jordan Kern

Electronic Theses and Dissertations

Walt Disney Studios possesses a checkered past in how its films dealt with racism and representation. Some of the earliest films involved songs and characters that go against modern sensibilities. In recent years, the studio's films have attempted to go against their forebears' racist connotations. Racism, however, proved a constant problem for the company. This paper shall explore the various ways Disney feature films addressed (or did not address) themes of racism and discrimination in its films from 1990 to 2018. The first chapter discusses the business reasoning behind Disney's continued reluctance to address race issues adequately, chiefly fear of …

The Role Of Src In Nsclc Cell And Lung Fibroblast Migration On 3d Cell-Derived Matrix., Austin M Krueger

Electronic Theses and Dissertations

Lung cancer is responsible for the most cancer deaths between males and females, with a 6% five-year survival rate. Cancer metastasis is one of the leading causes of lung cancer lethality. Our lab has developed a method to culture cells on cell-derived matrix (CDM) and study cell-ECM interactions. This study utilized multiple cell migration methods to investigate the migration of NSCLC cells and IMR90 lung fibroblasts, both together and independently, on 2D plastic and 3D CDM. Our results showed that inhibiting Src, a significant influencer of cell migration and member of integrin activation, blocked cancer cell migration on 2D and …

The Medici Example: How Power Creates Art And Art Creates Power, Margaret Hayden

Electronic Theses and Dissertations

This project looks at two members of Florence’s Medici family, Cosimo il Vecchio (1389-1464) and Duke Cosimo I (1519-1574), in an attempt to assess how they used the patronage of art to facilitate their rule. By looking at their individual political representations through art, the specifics of their propagandist works and what form these pieces of art came, it is possible to analyze their respective rules. This analysis allows for a clearer understanding of how these two men, each in very different positions, found art as an ally for their political endeavors. While they were in power only one hundred …

Not All Pollinator Gardens Are Created Equally: Determining Factors Pertinent To Improving Pollinator Garden Effectiveness, Travis Watson

Electronic Theses and Dissertations

Increasing evidence documenting the decline of insect populations, resulting from increasing human disturbances has resulted in efforts to establish pollinator gardens to provide additional resources for insect populations. However, our understanding of biotic and abiotic garden characteristics important for attracting and sustaining pollinator diversity is limited. Here, we evaluated 17 pollinator gardens to evaluate the effect of five biotic and three abiotic garden characteristics on pollinator species richness, abundance, and proportional representation of four pollinator functional groups. Plant species richness positively influenced pollinator richness and negatively influenced flower visitation. Bombus proportional abundance responded to several variables (distance to vegetation, plant …

If You Can Dream It, You Can Do It: Walt Disney’S Hero’S Journey To Professional Identity, Charles Mccoin

Electronic Theses and Dissertations

The purpose of this multi-method single-case study was to examine the application of Joseph Campbell's Heroic Journey model as a means of professional identity creation in the life of Walt Disney.

Walt Disney was an entrepreneur, cartoonist, filmmaker, inventor, studio head, and family man whose career stretched through the first half of the 20th century. Walt used his imagination and creativity to establish industry norms in the animation, film, television, and amusement park industries. Walt Disney's legacy and vision continue to be a viable influence within the Walt Disney Company today.

Campbell's Heroic Journey model was used as the theoretical …

Fanfiction: A Look Into The Disruptions Of Gender Identity Through Tropes, Stephanie Preslar

Electronic Theses and Dissertations

Fanfiction provides the unique opportunity to explore disruptions of heteronormativity through tropes. By exploring different fanfictions in the Soulmate AU, ABO Dynamics, and Mpreg tropes, the disruptions indicate a desire to explore gender, identity, and sexuality through queered characters. Male slash fanfiction provides the chance to examine the disruption of the heteronormative through the queering of male characters and placing them into situations that may embrace the feminine or a female-gendered experience. The situating of heterosexual male characters into queered roles allows an examination of how this disrupts canonical ideas of gender, identity, and sexuality. By reviewing the male slash …

Intuition Of An Outsider: From Nothing To Voice In George Scarbrough’S Poetry, William Moore

Electronic Theses and Dissertations

Long acknowledged as a committed poet of place, this thesis examines tones of outsiderness and alienation that characterize George Scarbrough’s poetry. Scarbrough draws on familiarity with his childhood in southeast Tennessee, and from an outsider’s outlook, a perspective veritably prompted by the rejection he suffered as a homosexual and lover of language, Scarbrough’s poetry addresses the daunting themes of fear and nothingness. Analysis of his poetry also reveals qualities of hope and endurance, a commitment to received forms, and Modern innovation. Through his poetic voice, culminating in the alter ego of Han-shan, Scarbrough provides vital insights into the human experience.

Morphology And Paleoecology Of Nimravides Galiani (Felidae) And Barbourofelis Loveorum (Barbourofelidae) From The Late Miocene Of Florida, Christianne Ormsby

Electronic Theses and Dissertations

Saber-toothed remains have been found worldwide throughout the Cenozoic, until the end of the Pleistocene. One site from Alachua County, Florida preserves a diverse Miocene fauna, including the machairodontine Nimravides galiani (Felidae) and the saber-toothed Barbourofelis loveorum (Barbourofelidae). Both taxa roamed what would become the Love Bone Bed site during the Late Miocene (Late Clarendonian NALMA), ~ 9.5 Mya. Previous descriptions focused on crania; yet the large sample of postcrania remained undescribed. Hence, this project includes a detailed postcranial description of both taxa. Results show that N. galiani resembles extant felids, whereas B. loveorum resembles Smilodon fatalis, as well …

Effects Of Respiratory Perturbations On Aging And Healthspan In Daphnia Magna, Millicent Nkiruka Ekwudo

Electronic Theses and Dissertations

Aging is a degenerative process characterized by a decline in physiological functions and cellular activities. Environmental and pharmacological interventions affecting longevity pathways have been extensively studied in model organisms. This study investigated the effect of chronic mild intermittent hypoxia (4 mg O2/L) or mild mitochondrial uncoupling with three doses of 0 (control), 0.1, 1, and 5 μM of 2,4-Dinitrophenol (DNP), on life history and gene expression in four clones of Daphnia magna. Interestingly, clones from intermittent ponds displayed better tolerance to hypoxia and DNP. Although neither treatments extended longevity, hypoxia increased fecundity and body size, and decreased food consumption and …

The Failure Of Chivalry, Courtesy, And Knighthood Post-Wwi As Represented In David Jones’S In Parenthesis, Taylor L. Hubbard

Electronic Theses and Dissertations

This thesis analyzes David Jones’s In Parenthesis to demonstrate the failed notion of chivalry, courtesy, and knighthood in modernity during and after the war. Jones’s semi-autobiographical prose poem recounting his experiences of WWI was published in 1937, nineteen years after the war ended. Jones applied the concepts of chivalry, courtesy, and knighthood to his experiences during WWI through In Parenthesis. Jones used these concepts, which originated in the classical period and the Middle Ages, to demonstrate how they have changed over time, especially given the events of WWI. The best way for Jones to demonstrate the impact of WWI …

The Effects Of Virtual Nature Exposure On State Social Motivation, Samantha A. Castelblanco

Electronic Theses and Dissertations

Social health is an important predictor of overall health. Yet, it is an often neglected area of research. Strikingly, social connectedness is associated with a 50% reduction in risk of early death. While a plethora of research evidence supports the beneficial impact of nature exposure on physical and mental health, literature regarding the beneficial impact of nature exposure on social health is scant. In fact, no research to date has investigated the causal influence of nature exposure on social motivation, a construct comprised here of three measures (State Motivation to Foster Social Connections, State Positive Affect, and State Anxiety). The …

Personification In Advertising: A Rhetorical Analysis Of Digital Video Ads In The Insurance Industry, Dorm Kpedor

Electronic Theses and Dissertations

Major companies in the insurance industry—notably Allstate, Progressive, and Farmers—often employ personification as a creative rhetorical tool in digital video advertisements. By leveraging brand characters in various ways, these companies seek to establish trust and engender emotional impact in customers. Allstate ascribes destructive characteristics that are associated with house cats to its Mayhem character; in doing so they evoke the desired emotional responses of humor and fear. Progressive creates and deploys the Motaur character, a visual personification and play on the Centaur; in this case, the company’s rhetorical strategy is to evoke humor and nostalgia that resonate with motorcycle owners. …

A New All-Natural Wound Treatment Gel Shows Strong Inhibitory Activity Against Staphylococcus Aureus And Other Wound Pathogens, Tasha K. Nelson

Electronic Theses and Dissertations

Skin related injuries are some of the most dangerous forms of wounds. In addition to treating the wound itself, health care providers must be cautious of microbial infections. In this study, we evaluate a novel all-natural antimicrobial gel compound (AMG) designed to kill planktonic bacteria, penetrate bacterial biofilms, and accelerate wound healing. In -vitro experiments demonstrate that AMG is effective in inhibiting planktonic growth and biofilm development of eight common pathogens. LIVE/DEAD staining and confocal microscopy reveal that planktonic growth and three-dimensional structure of biofilms were significantly reduced. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was used to investigate a small …

Character Influence And Brand Relationships: How Morally Ambiguous Characters Shape Brand Identity?, Jason Alexander Daugherty

Electronic Theses and Dissertations

This study examines morally ambiguous characters in television dramas and they represent their respect brand. The goal of the study is to examine how these MACs define their respective brands, specifically the brands, AMC and Showtime. Recent studies observed people chose brands based on their affective disposition and social cognition towards specific influencers. These theories could inform how people relate to characters from TV shows and brands. An observation and narrative analysis of four characters promotional videos from AMC were Don Draper (Mad Men), Saul Goodman (Better Call Saul) and from Showtime Jackie Peyton (Nurse Jackie) and Dexter Morgan (Dexter). …

Gravel Geology And Muskoxen Paleontology Of A Late Pleistocene Fossil Site In Saltville, Virginia, Nickolas A. Brand

Electronic Theses and Dissertations

Two distinct studies within the Saltville Valley of southwestern Virginia revealed insights into local Pleistocene geology and paleontology. A variety of analytical techniques were applied to gravel deposits within the paleontological site of SV-5/7 that revealed this unit is very poorly sorted, has a subangular matrix, and contains significant components of silt and sand in addition to rounded cobbles. These results suggest that rather than being deposited by fluvial processes as previously suggested, these gravels were likely the result of one or many debris flows. Additionally, the identity of fossil muskoxen from Saltville was reassessed using cranial and dental material. …

The Camouflaged Crime: Perceptions Of Poaching In Southern Appalachia, Randi Miller

Electronic Theses and Dissertations

The purpose of this study was to explore perceptions of poaching within the Southern Appalachian Region. To date, little research has been conducted on the general topic of poaching and no studies have focused on this Region. Several research questions were pursued, including perceptions of poacher motivations, methods and concern regarding apprehension and punishment. The study gathered data through qualitative interviews with 27 conservation officers and outdoorsmen located in Southern Appalachia to address these questions. Results provided useful information regarding the unique characteristics of the poaching problem within the Region, which are discussed in detail. Implications and directions for future …

Zeta Function Regularization And Its Relationship To Number Theory, Stephen Wang

Electronic Theses and Dissertations

While the "path integral" formulation of quantum mechanics is both highly intuitive and far reaching, the path integrals themselves often fail to converge in the usual sense. Richard Feynman developed regularization as a solution, such that regularized path integrals could be calculated and analyzed within a strictly physics context. Over the past 50 years, mathematicians and physicists have retroactively introduced schemes for achieving mathematical rigor in the study and application of regularized path integrals. One such scheme was introduced in 2007 by the mathematicians Klaus Kirsten and Paul Loya. In this thesis, we reproduce the Kirsten and Loya approach to …

Plprepare: A Grammar Checker For Challenging Cases, Jacob Hoyos

Electronic Theses and Dissertations

This study investigates one of the Polish language’s most arbitrary cases: the genitive masculine inanimate singular. It collects and ranks several guidelines to help language learners discern its proper usage and also introduces a framework to provide detailed feedback regarding arbitrary cases. The study tests this framework by implementing and evaluating a hybrid grammar checker called PLPrepare. PLPrepare performs similarly to other grammar checkers and is able to detect genitive case usages and provide feedback based on a number of error classifications.

Virtual Reality For Public Speaking Rehearsals: Student And Faculty Perceptions, Carrie A. Saltsman

Electronic Theses and Dissertations

Speeches and speech rehearsals are an important part of many basic communication courses. However, instructors are developing new ways for students to rehearse their speeches every day; one way may be for students to use virtual reality. Although virtual reality is somewhat of a new innovation, it is effective in various settings, including higher education, and more specifically, the speech classroom. However, this efficacy may not matter if students and instructors are unwilling to adopt it into the classroom. Diffusion of innovation theory, developed by Rogers (2003), focuses on how an innovation is diffused and adopted. Using two of the …

Exploring The Prevalence Of Social Isolation In Students At A Christian College In West Texas, David Bryan Elliott

Electronic Theses and Dissertations

The concept known as “social isolation” is a problem that affects many people, but there are certain populations that do not have adequate research performed on them. Specifically, college students face a risk of social isolation that is detrimental to their well-being and academic success, and there is a gap in the literature about this relationship. This study explored the prevalence of social isolation in college students at a Christian college in West Texas and examined various factors to determine whether any protective factors or at-risk factors existed. An online survey was sent out that used 11 demographic factor questions …

Barriers To Succession Planning: A Case Study Of Atlanta Nonprofit Organizations, Adonnis J. Jules

Electronic Theses and Dissertations

Succession planning is not a common practice among nonprofit organizations, despite support for its effectiveness in supporting leadership development and providing organizational stability during a planned or unplanned transition. Nonprofits have acknowledged that their leadership ranks are thin, and a leadership transition tends to bring challenges to the organizations when vacancies need to be filled. Barriers exist that prevent nonprofits from engaging in succession planning, but little research has examined the nature of these barriers. This study focused on nonprofit organizations across four different industry sectors to determine the extent of their succession planning efforts and the barriers that exist …

Warrior Dialogue In Samuel: Honor, Shame, And Divine Proxy, Robert A. Kranz

Electronic Theses and Dissertations

Discourse between warriors plays a role in several battle scenes within the books of Samuel. The repartee is a way for the narrator to add color and depth to the narrative and develop essential themes. Social-scientific criticism has often examined these interactions through the cultural lenses of honor and shame. Peristiany, Bourdieu, and Pitt-Rivers are just some scholars who developed cultural frameworks based upon the importance of honor and shame in the Mediterranean region. Malina, extrapolating from North African cultural studies by Bourdieu, developed a challenge and response model for the acquisition of honor as depicted in the Bible. However, …

The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr.

Electronic Theses and Dissertations

Native bees are an important part of terrestrial ecosystems due to their coevolution with flowering plants. This study catalogued the bee fauna of two sandyland sites in the Big Thicket National Preserve and assessed whether a community was impacted by a historic hurricane-induced flooding event. It was hypothesized that a change in diversity metrics would be evident following the flood. Datasets were analyzed for differences in species richness, abundance, evenness, and Shannon’s diversity. Similarities between datasets were also assessed using the multivariate tests analysis of similarity (ANOSIM) and similarity of percentages (SIMPER). At two sites over two years of sampling …