Digital Commons Network^™

Effects Of Electrical Stimulation And Testosterone In Translational Models Of Peripheral Nerve Injury, Gina Monaco

Dissertations

As functional recovery following peripheral nerve injury is dependent upon successful regeneration and target reconnection, combinatorial treatments that enhance different regeneration events may be required for recovery from severe injuries. The neurotherapeutic effects of electrical stimulation (ES) and gonadal steroids have been demonstrated independently and in combination in extratemporal and intratemporal facial nerve injuries. The goals of the first aim were to develop a reliable intracranial facial nerve crush model and to investigate the therapeutic potential of combining ES with testosterone propionate (TP) in this most proximal injury model. Adult male rats were divided into intracranial sham-operated, intracranial crush, and …

Acculturative Stress And Coping Strategies Used By Asian Indians Living In The United States: A Quantitative And Qualitative Inquiry, Ritu V. Thaker

Dissertations

The present research addressed acculturative stress experiences and coping strategies used by Asian Indians residing in the United States. The organizing research question was "What are the acculturative stress experiences and coping strategies used by first generation Asian Indians living in the United States?" The research question was explored through fourteen hypotheses.

A mixed method approach was employed. For the first phase, 54 first-generation Asian Indians were recruited from four sites using a purposive convenience sampling method, the SAFE-R standardized questionnaire was used to measure acculturative stress, the COPE scale to measure coping strategies, and a culturally grounded Socio-Demographic Information …

A Study Of The Therapeutic Potential Of Af4 Mimetic Peptides, Nisha N. Barretto

Dissertations

Mixed lineage leukemias (MLL) are a group of acute and aggressive leukemias. They account for over 70% of infant leukemias, and 10% of acute adult leukemias. Pediatric ALL and therapy related MLL leukemias carry poor prognosis in spite of several advancement in the field of leukemia research. Therefore, new therapies for MLL leukemias are needed.

Majority of MLL leukemias arise due to the balanced translocations of the MLL gene. As a result of these translocations, chimeric MLL fusion proteins are expressed. The most frequently occurring MLL fusion proteins are known to aberrantly recruit the super elongation complex (SEC) resulting in …

Biophysical Characterization Of Tryptophan Locales, Mg²+ Binding And Protein Folding In Gα Subunits, Matthew Najor

Dissertations

The objective of this study is to understand the structure of guanine nucleotide - binding (G) proteins using a variety of spectroscopic tools. G proteins are membrane-bound proteins consisting of α, β, and γ subunits required for the transduction of extracellular signals to various intracellular effectors. Activation of G protein coupled receptors by neurotransmitters or hormones result in a conformational change of a G protein that is triggered by the exchange of guanosine 5'- diphosphate (GDP) bound to the  subunit for guanosine 5'- triphosphate (GTP) and concomitant dissociation of the  dimer.

Wild type (WT) Giα1 has three tryptophan …

Particulates Initiate Immune Response Via Inducing Oxidative Stress-Mediated Nlrp3 Inflammasome Activation, Zhenyu Zhong

Dissertations

Liposomes are lipid particles formed by self-aggregated phospholipids. They are clinically used both as delivery vectors and immune adjuvants to treat many human infectious and cancerous diseases. However, how liposomes interact with host immune system remains poorly understood. Particularly, little is known about whether an innate immune receptor exists to sense liposomes. I hypothesize that liposomes activate NLRP3 inflammasome in macrophages, which results in the release of interleukin 1β (IL-1β), a proinflammatory cytokine that plays a key role in innate and adaptive immune responses.

To test my hypothesis, I determined the ability of inducing IL-1β secretion from macrophages by differentially …

Comprehensive Investigation Of The Transcription Profiles Of Solanum Lycopersicum Treated With Two Cerato-Platanins From Botrytis Cinerea, Talitha Caudle

Dissertations

Botrytis cinerea is a severe plant pathogen that destroys more than 200 crop species worldwide. During early infection, B. cinerea secretes a large store of proteins onto the host surface, many of which cause acute necrosis, senescence, and the loss of cell wall integrity. Recently, cerato-platanin (CP) family member BCSNOD1 was found among fungal secretory products. A subsequent expression profile of B. cinerea-infected strawberry showed the early induction of BCSNOD1 and the CP homologue BCSNOD2. The function of CPs is poorly understood, although evidence suggests a role in plant-pathogen interactions. To characterize CP during B. cinerea infection, the interactions of …

Phytochemical Investigation Of Lonchocarpus Species : A Search For Antioxidant Compounds, Caitlin E. Deskins

Dissertations

Natural product research plays a crucial role in the discovery of new drugs for the treatment of human diseases. One plant genus that has been shown to contain a variety of compounds with many different activities but has yet to be thoroughly investigated is the genus Lonchocarpus (Fabaceae, Papilionoideae). In this work, three species of Lonchocarpus from Monteverde, Costa Rica were investigated. Each of the species underwent antioxidant-guided fractionation to isolate compounds that are responsible for the extracts' antioxidant activity. Antioxidant activity was evaluated using two antioxidant assays, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay and the ferric reducing ability of plasma …

Ligation-Dependent Multiplex Pcr With Bead-Based Array Detection Comprises A Clinically Adaptable Method Of Microrna Profiling, Bobbi Ellen Johnston

Dissertations

Ligation-dependent PCR, when coupled with a bead-based detection array, comprises a method of nucleic acid detection that utilizes enzymes and equipment already being employed successfully in clinical labs. The process can be multiplexed in order to generate a profile of the sequences of interest. When applied to microRNA profiling, the ligation step requires modification to improve the efficiency of the T4 DNA ligase employed. With optimization, the assay has the potential to distinguish and detect multiple microRNA species simultaneously, providing a clinically adaptable method for microRNA profiling. The flexibility of the assay format would allow modification for a nearly limitless …

Effects Of Plasma Jet Parameters, Ionization, Thermal Conduction, And Radiation On Stagnation Conditions Of An Imploding Plasma Liner, Miloš Stanić

Dissertations

The disciplines of High Energy Density Physics (HEDP) and Inertial Confinement Fusion (ICF) are characterized by hypervelocity implosions and strong shocks. The Plasma Liner Experiment (PLX) is focused on reaching HEDP and/or ICF relevant regimes in excess of 1 Mbar peak pressure by the merging and implosion of discrete plasma jets, as a potentially efficient path towards these extreme conditions in a laboratory. In this work we have presented the first 3D simulations of plasma liner, formation, and implosion by the merging of discrete plasma jets in which ionization, thermal conduction, and radiation are all included in the physics model. …

Analysis Of Dna Methylation And Copy Number Variation In Renal Cell Carcinoma, Brittany N. Lasseigne

Dissertations

Renal cell carcinoma (RCC) is the 10th most commonly diagnosed cancer in the United States and its incidence is increasing. It is difficult to detect early and is relatively non-responsive to traditional radiation and chemotherapies. Previous work demonstrates the value of measuring copy number variation (CNV) and DNA methylation changes in RCC, but diagnostic biomarkers and additional treatment options in the clinic are still needed. This study examined genome-wide DNA methylation and CNV in RCC tumor and benign adjacent kidney tissues from 101 clinically-annotated patients. Analyses revealed widespread DNA methylation and copy number differences between tumor and benign tissues, particularly …

Chemical Investigation Of Opuntia Species And Stenocereus Thurberi, Cacti Native To North America : A Search To Identify Essential Oil Compositions And To Characterize Cyclic Siloxane Compounds, Cynthia Rena Wright

Dissertations

This study investigates the essential oil compositions of certain cacti species native to southern Arizona and the California Channel Islands. The species studied are Opuntia littoralis (Engelm.) Cockerell., Opuntia ficus-indica L. (Mill), Opunita prolifera Engelm., Opuntia acanthocarpa var. major (Engelm. & J.M. Begelow) L.D. Benson, Opuntia phaeacantha var. discata, and Stenocereus thurberi Engelm. Drosophila mojavensis, a cactus fly, utilizes the necrotic rots of O. littoralis and S. thurberi, which have been shown to be toxic to fruit flies, Drosophila melanogaster. The oil compositions of both O. littoralis and S. thurberi was characterized at differing states of necrosis and compared to …

Critical Functions Specified By The Mll Cxxc Domain Determine Leukemogenic Capacity, Noah Warren Birch

Dissertations

TheMixed Lineage Leukemia(MLL) gene can participate in chromosomal translocations which generate a fusion protein leading to acute leukemia. A better understanding of how MLL fusion proteins contribute to leukemia is necessary in order to develop more effective treatments. In my dissertation project, I investigated the functional role of amino acids within the MLL CXXC domain to determine how specific residues contribute to leukemogenic capacity.

MLL fusion proteins retain the amino-terminal portion of MLL including the CXXC DNA-binding domain while the carboxy-terminal portion is comprised of a fusion partner. The closest homolog of MLL, MLL2 (alternatively named MLL4), also contains a …

Toward The Development Of The Stereotypical Roles Of Black Young Men Scale, Amber Hewitt

Dissertations

There is a significant amount of literature on identity development in general, but there is a dearth of research focusing on identity development in relation to how other processes and constructs influence the identity development of African American young men. One such construct is the presence of stereotypical roles. The primary purpose of this study was to create a reliable and valid measure of the stereotypical roles of African American young men. This study explored the relationship between the endorsement of stereotypical roles, stigma consciousness, and masculinity of African American young men. African American young men (n = 164) between …

Psychosocial Distress Mediates Immune Dysregulation Through Alterations In Global Epigenetic Patterns And Chromatin Remodeling Proteins, Karen Krukowski

Dissertations

Psychosocial distress, characterized by increased perceived stress, anxiety and mood disturbance, is a common response of women to a diagnosis of breast cancer (Northouse, 1992; Pettingale et al., 1988; Stark and House, 2000; Witek-Janusek et al., 2007). This psychosocial distress leads to activation of the hypothalamic-pituitary-adrenocortical (HPA) axis and increased circulating glucocorticoids (GCs) (Chrousos, 2000; Chrousos and Gold, 1992; Schoneveld and Cidlowski, 2007). Increased psychosocial distress and increased HPA activation can lead to immune dysregulation consisting of reduced natural killer (NK) cell activity (NKCA) (Biondi, 2001; Kiecolt-Glaser et al., 1987; Kiecolt-Glaser et al., 2002; Witek-Janusek et al., 2008; Witek-Janusek et …

Open Enrollment In Advanced Placement Courses: Experiences Of Traditional And Non-Traditional Students, Scott Errol Mcalister

Dissertations

Beginning in the late 1990's, amongst concern that traditionally underrepresented groups of students such as minorities and low income students had been denied the opportunity to enroll in AP courses, The College Board (the non profit agency that runs the AP program) began to emphasize its belief that all students should have the right to take AP courses if they desire and that secondary schools should not limit AP enrollment based on prerequisites such as teacher recommendation, GPA, and class rank. As a result of these factors, the AP program began to see dramatic gains in the number of students …

Effects Of Acute Alcohol Exposure On Post Burn Intestinal Immunity: Role Of Il-23, Juan L. Rendon

Dissertations

More than one million burn injuries are reported yearly within the United States. These injuries result in approximately 500,000 emergency room visits and 40,000 hospitalizations annually. Greater than 50% of these injuries occur under the influence of alcohol/ethanol (EtOH) intoxication. Burn victims who sustain injury under the influence of EtOH exhibit significantly higher rates of morbidity and mortality than patients without EtOH exposure at the time of injury. Several lines of evidence suggest that gut pathogens and/or their products may play a role in the development of sepsis and multiple organ failure reported in burn and trauma patients. In line …

Just Like Everyone Else? Locating South Asians In 21st Century American Popular Media, Bhoomi K. Thakore

Dissertations

In the 21st century, representations of South Asians in American popular media have grown exponentially. This is a particularly interesting phenomenon when considering the status that South Asians occupy in the American racial hierarchy. In Just Like Everyone Else? Locating South Asians in 21st Century American Popular Media, I examine audience perceptions of South Asian/Indian characters and actors in American popular media. My research is guided by the following question: to what extent do audience perceptions of South Asians in U.S. popular media reflect pre-existing ideologies of race, gender, ethnicity, and immigration? Using data from 155 open-ended online questionnaires and …

Remembering The Cultural Trauma Legacies Of Slavery: African American Young Adult Perceptions On Racism, Ethnic Identity, And Racial Socialization, Kimya Pearl Barden

Dissertations

The purpose of this research investigation is to explore cultural trauma theory on African American young adult development. Cultural trauma theory asserts the adverse cross-cultural encounter, North American slavery, reproduces intergenerational psychosocial legacies for contemporary African Americans. Accordingly, cultural trauma theory is used to explore with African American young adults three "slave" legacies: ethnic identity formation, perceptions of racism, and racial socialization experiences. A qualitative case study approach is used for (N=26) participants enrolled in either college or a GED program. Each young adult participates in either an individual or focus group interview. To aid in data triangulation, older African …

Student Achievement In Middle Grades: Gauging The Effect Of Teacher Training On Student Learning, Natalya Gnedko

Dissertations

In the United States, teacher certification has been a baseline measure of teacher quality and gateway to the teaching profession for many decades. Research suggests that teacher certification is beneficial to student achievement, with findings particularly promising when content area of teacher certification is taken into account. Positive findings are largely limited to high school. However, in recent years middle grades have been increasingly viewed as a starting point for success in high school and college, which led many school districts to require advanced training for middle grades teachers. To examine the effectiveness of advanced teacher training in middle grades, …

Perceived Social Class, College Interest, And Post-Secondary Goals: An Application Of The Scat Interest And Choice Model, Jason Daniel Hacker

Dissertations

The purpose of this study was to investigate the role of perceived social class (PSC) in the educational development of 176 racially and economically diverse high school students. PSC was defined based on the tenants of differential status identity theory (Fouad & Brown, 2000) and then incorporated as a person variable in the interest and choice model of social cognitive career theory (Lent & Brown, 1996; Lent, Brown, & Hackett, 1994). The study first examined the relation of PSC to students' choice intention to pursue a college degree via cognitive self-evaluations (college self-efficacy, college outcome expectations, college interest). Alternatively, PSC …

Targeting The Notch-1/Igf-1r/Akt Axis In At Orthotopic Model Of Advanced Non-Small Cell Lung Cancer, Shuang Liang

Dissertations

Lung cancer is the leading cause of cancer death in the U.S. and worldwide. The most frequent type of lung cancer is non-small cell lung cancer (NSCLC). NSCLC is mostly diagnosed at advanced stages (stage IIIB 18% of cases, stage IV 40% of cases) due to the lack of effective early detection methods. Thus, the discovery of alternative therapeutic strategies is of extreme importance.

Others and we have previously found that Notch signaling plays a crucial role in NSCLC. Our preliminary results indicate that Notch-1 provides necessary survival signals to NSCLC cells by positively regulating IGF-1R to activate the Akt-1 …

Feeling Like A Holy Warrior: Western Authors' Attributions Of Emotion As Proof Of Motives For Violence Among Christian Actors In Military Conflicts, Tenth Through Early Twelfth Centuries, Jilana Ordman

Dissertations

This dissertation explores two areas of human experience that have been criticized as potentially dangerous and uncontrollable almost consistently since Late Antiquity: violence and those who engage in it, and emotions. However, it will be seen that in the Western Mediterranean and Southern and Central Western Europe, from Late Antiquity through the early-Twelfth Century, these areas were carefully controlled and directed by complex philosophical and religious systems.

Polytheist Roman, and later patristic Christian, authors who wrote within classical and late antique philosophical and religious systems created the accepted norms for the undertaking of organized violence - that which was fought …

Effectiveness Of Graduate Training In School Psychology: Perspectives Of Graduate Practitioners, Mary S. Satchwell

Dissertations

While trends in graduate training in school psychology indicate a movement towards an increasing emphasis on consultation and decreasing emphasis on assessment (Anton-LaHart & Rosenfield, 2004), there remains a gap between training and practice for professional school psychologists (Harrison, et al., 2004). The present study provided an examination of practitioner views for graduates of both specialist and doctoral training programs from 2006 through 2011 at a single NASP-approved, NCATE-accredited university setting. The primary purpose of this study was to examine alumni practitioners' perspectives of the effectiveness of their coursework and fieldwork experiences in preparing them for their professional roles as …

The Effects Of Acculturation, Health, Socioeconomic Status, And Perceived Respect On Older Adult Depression: Analysis Of Korean American Older Adults In Chicago, Kyungsoo Sim

Dissertations

This study explored the extent to which acculturation level, health condition and SES influence depressive symptoms mediated or moderated by perceived respect and current geographical background of 121 Korean-American older adults in the Chicago metropolitan and its suburban area.

A multi-method approach was imported to analyze self-collected quantitative survey data from two groups of 80 participants in Chicago and 41 in the suburbs, qualitative interview data from 4 focus groups, 2 in-depth interviews and GIS (Geographic Information Systems). In the survey, Suinn-Lew Asian Stress-Identity Acculturation Scale, Geriatric Depression Scale-30, Functional Assessment of Chronic Illness Therapy-Fatigue, Health Perception Questionnaire-36, 13-item questions …

Characterization Of Chronic Glucocorticoid Exposure On Natural Killer Cell Function: A Model For Stress Induced Alterations On The Epigenetic Landscape, Justin Lee Eddy

Dissertations

Psychological stress can impact immune function through altered glucocorticoid production. Natural killer (NK) cells and their effector functions are particularly susceptible to both psychological stress as well as exogenous glucocorticoids. The purpose of this study was to assess the impact of chronic glucocorticoid (GC) exposure on NK cells to investigate the immune dysregulation associated with periods of psychological stress.

Chronic in vitro treatment of the NK92 cell line produced a dichotomous NK phenotype with enhanced proinflammatory cytokine production yet reduced NK cell lytic activity (NKCA). This dichotomy was at the individual cell level where mRNA and protein levels for proinflammatory …

Ex Vivo Induction Of Human Regulatory T Cells, Kathleen Elizabeth Jaeger

Dissertations

Regulatory T cells (Tregs) are indispensable in the human immune system in the maintenance of self-tolerance and immune homeostasis. The dysregulation of Treg number and/or function plays a critical role in the pathogenesis of cancer, allergy and human autoimmune pathologies such as multiple sclerosis, psoriasis, rheumatoid arthritis and diabetes. The primary aim of this research was to determine the molecular mechanisms and cellular interactions which promote human Treg induction. From umbilical cord blood (UCB) samples, we found that antigen receptor stimulation of UCB T cells via a CD14+ monocyte population leads to a predominant induction of Tregs.

Further studies identified …

The Effects Of An Experiential Learning And Mentorship Program Pairing Medical Students And Persons With Cognitive Impairment: A Qualitative Content Analysis, Darby J. Morhardt

Dissertations

The United States population is aging rapidly and with it a tremendous rise in the number of people with dementia. In the future, as now, physicians and other health care professionals will likely provide the majority of health care for older people and those with dementia. The non-geriatric trained workforce must have the attitudes, knowledge and skills needed to provide high quality care for this aging and increasingly cognitively impaired population. There is evidence that prevailing stigma regarding older adults can be modified with experiential opportunities early in students' career. Few of these programs are aimed at persons with dementia. …

Defining A Role For Inducible Heat Shock Protein 70 (Hsp70i) In Mediating Autoimmune Vitiligo, Jeffrey Alan Mosenson

Dissertations

Vitiligo is an autoimmune disease characterized by destruction of melanocytes, leaving 0.5% of the population with progressive depigmentation. Current treatments offer limited efficacy. Observations that heat shock proteins (HSPs) can serve as adjuvants in antitumor vaccines first suggested to us a link between HSPs and stress-induced vitiligo. Such proteins are marvelously well conserved throughout evolution, which has placed them in the spotlight for helping to understand the intriguing relationship between infection and immunity. Intracellularly, inducible heat shock protein 70 (HSP70i) is upregulated by stress and helps protect cells from undergoing apoptosis. In times of stress, melanocytes will secrete antigen bound …

The Religion Of Consumption And Christian Neighbor Love, Christopher Porter

Dissertations

Loyola University Chicago

THE RELIGION OF CONSUMPTION AND CHRISTIAN NEIGHBOR LOVE

Consumerism is a word frequently used in various disciplines to express the variety of attitudes, motivations, and practices found among the middle and upper classes. It drives the global economy and influences individuals' socio-psychological perceptions. Some have gone so far as to call consumerism a religion, yet they have not substantiated this claim. This dissertation offers a framework that accounts for consumerism as a religion both as a person's ultimate concern and as a structuralized belief system. As such, it prescribes moral values that shape how people respond to …

Structure And Mission Effectiveness: A Study Focused On Seventh-Day Adventist Mission To Unreached People Groups Between 1980 And 2010, Abraham Guerrero

Dissertations

The present study examines the impact of the Seventh-day Adventist Church's structure on mission effectiveness in taking the gospel to unreached people groups between 1980 and 2010. A historical descriptive study, this dissertation's theory base includes structure from an anthropological perspective; structure, mission, and effectiveness from an organizational perspective; and church structure and mission effectiveness in Christian history.

The impact of structure on mission effectivenessis evaluated in the present work by looking for patterns in history in which the structure has been either a facilitator or a hindrance for establishing churches among those who have not been reached with the …