Digital Commons Network^™

The Rate And Process Of Mangrove Forest Expansion On Above- And Belowground Carbon Relations In Coastal Louisiana, Hai Thanh Pham

Dissertations

Global climate change is a major environmental threat to natural and cultural resources in low-lying coastal zones and deltas worldwide. It is expected to have a significant effect on the development of coastal wetlands by changing species range expansion. In fact, recent studies have shown that there has been an increasing mangrove encroachment poleward in the Southern and Northern Hemispheres into temperate saltmarsh zones due to the lack of freeze events. This phenomenon could have a critical impact on the structure and function of tidal wetlands at critical latitudes, especially in carbon sequestration, since mangroves are thought to sequester more …

Direct Writing Of Polymeric Coatings For Corrosion Control And Tunable Release Of Bioactive Materials, Eben Adarkwa

Dissertations

The interface between a medical device and its surrounding tissue can be critical to biocompatibility, performance and therapeutic effectiveness. Careful choice and application of materials at this interface is therefore a key to the success of any medical device. This research employed a novel direct-write inkjet printing technique for polymeric surface modification of bioresorbable AZ31 Mg alloy towards corrosion control and tunable release of bioactive agents. In the first phase of this research, the direct-write inkjet printing technique was successfully used to fabricate thin films of different blends of poly (ester-urethane) urea embedded with taxol coatings on mechanically polished AZ31 …

The Correlation Between The Geographical Region Of A High School And The Enrollment Ratio Of Males To Females In Accelerated College-Preparatory Level Mathematics Courses, Mary Anne Latragna

Dissertations

Females, in this modern age of feminism, have excelled in all fields of study and graduate from college in larger numbers than males. However, few women go into the STEM fields (Hill, Corbett, & St. Rose, 2010). To close this gap in mathematics-related fields, it is paramount that high schools produce female students who are advanced in Mathematics. The problem was the underrepresentation of females in mathematical fields of study, with a more pressing issue of underrepresentation of females in college preparatory mathematics classes at the high school level. The purpose of this study was to determine if the geographical …

Coronavirus Proteases As Therapeutic Targets: Development Of Biosensors To Detect Inhibition Of Protease Activity And Separation Of The Multiple Functions Of Coronavirus Papain-Like Proteases, Andrew Kilianski

Dissertations

Coronaviruses are important human pathogens and have the potential to severely impact public health on an international scale. The emergence of SARS-CoV and MERS-CoV highlight the need for research to identify antivirals and vaccines against coronaviruses. To develop therapeutics against current and potentially emergent coronaviruses, I utilized two approaches targeting the proteases encoded within all coronaviruses. The papain-like protease and 3C-like protease of coronaviruses are responsible for cleaving viral polyproteins early during infection, and this step is required for viral replication. To quantitatively assess the inhibition by small-molecule compounds on MERS-CoV protease activity, I developed a luciferase-based biosensor to monitor …

The Restorative Justice Talking Circle Process With Probation Officers: A Phenomenological Approach, Constance Marie Sheehan

Dissertations

Restorative justice seeks to bring together stakeholders in the justice system by acknowledging harm caused by offending behavior and finding ways to repair that harm. The activities associated with restorative justice create the potential for meaningful and positive outcomes for all stakeholders within the system. Restorative justice asks, what is the harm?, who are the stakeholders?, and, how can the harm best be repaired? A particular restorative practice - the talking circle - is a common practice of restorative justice efforts. The potential for restorative justice practices, like the talking circle, is developing of a new way of understanding for …

Regulation And Targeting Of Fyn In Human Cutaneous Squamous Cell Carcinoma, Sarah Fenton

Dissertations

Fyn, a member of the Src family kinases, is an oncogene in murine epidermis and is associated with cell-cell adhesion turnover and migration. Introduction of active H-Ras(G12V) into the HaCaT human keratinocyte cell line resulted in upregulation of FYN mRNA (200-fold) and protein, but did not increase the expression of other SFKs. We identified two nucleotides 74 bases upstream of the human FYN gene transcription start site as necessary for FYN upregulation in HaCaT-Ras cells. Further studies identified Lef-1 as the transcription factor most likely binding to this site and mediating FYN upregulation.

Transduction of active Ras or Fyn was …

The Role Of Af9 And Af9-Mediated Protein Interactions In Hematopoiesis And Leukemogenesis, Alyson Anne Lokken

Dissertations

The AF9 protein is one of the most common chromosomal translocation partners of the MLL gene in MLL leukemia. Wild-type AF9 is a member of the pTEFb transcription elongation complex, and interacts with gene regulatory proteins such as AF4/AF5q31, DOT1L, Pc3/CBX8 and BCoR. These interactions are retained in the oncogenic MLL-AF9 fusion protein, and may be required for leukemic transformation.

Using bone marrow progenitor cells isolated from conditional Af9 knockout mice, we examined in vitro differentiation of hematopoietic progenitor cells to the erythroid, myeloid and megakaryocytic lineages in the presence or absence of Af9. Based on previously published studies, we …

Traveling Wave Solutions Of A Chemotaxis Model : Existence And Stability, Reem N. Albashaireh

Dissertations

In this dissertation, we investigate the existence and stability of traveling wave solutions of a chemotaxis model system, which consists of two reaction-diffusion equations, of which containing the chemotaxis term. We prove the existence of a family of traveling wave solutions, one for each wave speed c > c_* for some c_* > 0. Due to continuity of these wave solutions on c, they are unstable in the Banach space X := C ( R ̅) × C(R ̅), where C(R ̅) is the Banach space, equipped with the uniform norm, of all continuous functions U on R with both U(±∞) being …

Integrated Consensus Based Frameworks For Unmanned Vehicle Routing And Targeting Assignment, Waleed T. Barnawi

Dissertations

Unmanned aerial vehicles (UAVs) are increasingly deployed in complex and dynamic environments to perform multiple tasks cooperatively with other UAVs that contribute to overarching mission effectiveness. Studies by the Department of Defense (DoD) indicate future operations may include anti-access/area-denial (A2AD) environments which limit human teleoperator decision-making and control. This research addresses the problem of decentralized vehicle re-routing and task reassignments through consensus-based UAV decision-making. An Integrated Consensus-Based Framework (ICF) is formulated as a solution to the combined single task assignment problem and vehicle routing problem. The multiple assignment and vehicle routing problem is solved with the Integrated Consensus-Based Bundle Framework …

Mindful Mending: The Repair Of Thought And Action Amidst Technologies, Bryan Kibbe

Dissertations

My thesis is that the concept and practice of repair, properly understood and circumscribed, can serve to: (1) specify a responsibility to care for individuals who are cognitively dependent on particular configurations of technologies and suffer cognitively significant harms following damage to various technologies, and (2) to act as a standard by which to regulate the design, implementation, and selection of technologies available for human use and appropriation. I begin (Chapters One and Two) by providing a critical investigation of the concept and practice of repair. In Chapters Three and Four, I set forth a proposal to consider what I …

Empowering Homeless Youth In Transitional Living Programs: A Transformative Mixed Methods Approach To Understanding Their Transition To Adulthood, Ashley Etzel Ausikaitis

Dissertations

The purpose of this dissertation is to gain insight into the journeys of homeless youth residing in transitional living programs in the Chicago area. There are multiple factors that can lead youth to homelessness as well as various risks made greater by living on the street that can lead to negative life outcomes. There is a dearth of research on outcomes of youth in transitional living programs, particularly research that includes the perspectives of those receiving services. This researcher partnered with three transitional living sites that serve homeless youth ages 18 to 24. Utilizing both qualitative and quantitative methods, the …

Catalytic Mechanism And Maturation Of The Metalloenzyme Nitrile Hydratase, Natalie I. Gumataotao

Dissertations

Nitrile hydratases are metalloenzymes that catalyze the hydration of nitriles to their corresponding amides in a specific manner at ambient pressures and temperatures at neutral pH. Traditional industrial methods require high temperature and pressure, extreme pH, and heavy metals. NHases are used as biocatalysts in the large scale industrial production of amide precursors to textiles, animal feedstock, and polymers. Notably, NHase is used in the production of ~100,000 tons of acrylamide annually by the Mitsubishi Corporation.

Despite being used extensively in industry, questions remain about NHase. The catalytic mechanism is not defined. Understanding the way in which the nitrile is …

Urban Agriculture And Sustainability In Chicago, Matthew Hoffmann

Dissertations

Sustainability is increasingly informing the organization of urban life. In the past 15 years, cities have sought to alleviate the dual effects of environmental degradation and a changing economy by introducing sustainable development initiatives. Policy makers and environmental activists have often praised these efforts, pointing to the enhancement of quality of life, the mitigation of industrial pollution, and these initiatives' capacity to address poverty in a post-welfare state. Most popular depictions of the experiences of sustainability come from the perspective of white, middle class, college-educated volunteers and, increasingly, social entrepreneurs such as artisans, farmers, and other producers connected to "local" …

Practitioners' Evaluations Of Theraplay As An Effective Tool In Serving Foster And Adopted Children And Their Families, Rana Hong

Dissertations

Several studies have shown that foster and adopted children have high risk of developing severe mental health problems (Blome & Steib, 2004; Golden, 2009; Leslie, Hurlburt, Landsverk, Barth, & Slyman, 2004; McMillen. Scott, Zima, Ollie, Munson, & Spitznagel, 2004). On the other hand, evidence-based models are limited and less accessible for practitioners working with foster and adopted children (Dorsey, Kerns, Trupin, Conover, & Berliner, 2012). The goal for this study is to explore the practitioners' evaluations of Theraplay®, which is a relationship based model for meeting the pervasive clinical needs of foster and adopted children and their families. Exploring Theraplay …

The Symptom Experience Of Patients Receiving Epidermal Growth Factor Receptor Inhibitors, Josephine Ann Howard-Ruben

Dissertations

This study explored the symptom experience of patients receiving epidermal growth factor receptor inhibitors (EGFRIs) for breast, colon, head and neck, and colon cancer. EGFRIs are targeted therapies used at various points along the treatment continuum for these solid tumors, and may be first, second or third-line agents which can be used as single agents or in combination with other therapies. The most common side effect of these agents include dermatologic effects, such as rashes, hair, and nail changes, but they can also contribute to other side effects such as fatigue, anxiety and diarrhea. Most previous work has addressed the …

Host Proteins Interact With The Hiv-1 Core To Facilitate And Restrict, Zana Lukic

Dissertations

Host cell proteins, termed restriction factors, which inhibit viral replication at various stages of the viral life cycle, determine the species-specific tropism of numerous retroviruses. Many members of the TRIM family of proteins act as viral restriction factors. One well-characterized example is the ability of TRIM5á from rhesus macaques (rhTRIM5á) to inhibit human immunodeficiency virus type-1 (HIV-1) soon after viral entry but prior to reverse transcription (RT). It is well established that the restriction requires an interaction between the viral capsid lattice and the B30.2/SPRY domain of TRIM5á. Following the binding of the viral core, TRIM5á mediates an event or …

The Impact Of Insulin Management On Quality Of Life In Type 2 Diabetes, Sandra Ellen Mccormick

Dissertations

Complications of type 2 diabetes (T2DM) are severe but can be minimized through excellent glycemic control, optimally achieved by using intensive, or basal-bolus, insulin management. The quality of life (QOL) effects of basal-bolus insulin management are not fully known. This cross-sectional, observational study was based on the Revised Wilson and Cleary Model for Health Related Quality of Life, which measures five QOL domains (biological function, symptoms, functional status, general health perceptions, and overall QOL). The study aims were to describe, compare, and predict QOL in persons with T2DM based on type of insulin management (oral meds only, basal insulin only, …

Moderating Variables In The Treatment Effects Of Second Step, Gregory Eugene Moy

Dissertations

Youth violence is a problem which has been studied through theoretical frameworks based on social ecological models of development. This has coincided with the development of programs intended to prevent and reduce the occurrence of youth violence and its negative effects. One approach to preventing youth violence has been to deliver social emotional learning programs to children and adolescents in school settings. Social emotional learning programs are designed to provide children with skills and strategies for increasing self awareness, social awareness, emotion management, problem solving, and thoughtful decision making. Increasing students' familiarity and use of these prosocial strategies are thought …

The Backgrounds And Outcomes Of Allopathic Medical School Applicants: Exploring Stratification And Inequality, Sunshine Nakae

Dissertations

A career in medicine remains one of the most rigorous and arduous journeys in higher education. The time and cost to train coupled with the academic and extracurricular requirements have resulted in challenges in diversifying the profession. This study utilizes an HLM and HGLM to model the influences of individual and institutional predictors on acceptance to medical school. Full descriptive analysis of individual and institutional predictors are also included.

Peripubertal Binge Ethanol, Age And Sex Modulate Microrna Expression In The Ventral And Dorsal Hippocampus Of The Adolescent Rat, Sarah Arianne Prins

Dissertations

Adolescent binge ethanol (EtOH) abuse induces long-term changes in gene expression, resulting in an increased risk for the development of adult mood disorders. microRNAs (microRNAs) are small, noncoding RNAs that regulate gene expression by translational repression. microRNA altered in response to EtOH and puberty in the developing brain. The biogenesis of mature 22-24 nucleotide (nt), single-stranded microRNAs involves Drosha and Dicer enzymatic processing of microRNA precursors. A mature microRNA imperfectly base pairs with messenger RNA (mRNA) target genes,which leads to mRNA cleavage or translational repression. Our lab has found that repeated binge EtOH exposure alters gene expression in the hypothalamus …

Extending Students' Practice Of Metacognitive Regulation Strategies In The Undergraduate Chemistry Laboratory And Investigation Of Pb2+ Binding To Calmodulin With Circular Dichroism And Molecular Dynamics Modeling, Mary Twist Van Opstal

Dissertations

The following dissertation was composed of two projects in chemistry education and benchwork/computational biochemistry. The chemistry education research explored students' practice of metacognitive strategies while solving open-ended laboratory problems when engaged in an instructional environment, the Science Writing Heuristic (SWH), that was characterized as supporting metacognitive regulation strategy use. Through in-depth interviews with students, results demonstrated that students in the SWH environment, compared to non-SWH students, used metacognitive strategies to a greater degree and to a greater depth when solving open-ended laboratory problems. As students engaged in higher levels of metacognitive regulation, their elective use of peers became a prominent …

Investigating The Role Of The Pgf2 Alpha/Calcineurin-Signaling Pathway In The Regulation Of Adipogenesis, Damodaran Annamalai

Dissertations

Prostaglandin F2α (PGF2α) is a potent physiological inhibitor of adipocyte differentiation. In previous studies, we demonstrated that PGF2α inhibits adipogenesis via activation of the calcium-regulated protein phosphatase, calcineurin. In this current study, we have now extended these findings to identify the IL-11 cytokine and the Nurr1 orphan nuclear hormone receptor as functionally important downstream transcriptional targets of the PGF2α/calcineurin-pathway involved in the inhibition of adipocyte differentiation. In the case of IL-11, we show that this cytokine acts in an autocrine fashion to inhibit adipogenesis via the essential actions of the gp130 cytokine co-receptor signaling subunit. Further, by using a well-characterized …

Speaking Of Right And Wrong: Black Girls And Moral Development, Tonya Bibbs

Dissertations

Our current approaches to moral development were generalized from the experiences of white males and females. This study draws upon existing theory in order to consider its relevance to Black girls' everyday lives. The study draws upon the moral reasoning gathered from ethnographic dilemma interviews with 8-11 year old girls and their mothers to develop a grounded theory of moral development. The contents of the resulting theory suggest that, while there are aspects of existing moral development theory that appear to be consistent across contexts, the particularity of Black community and middle childhood culminate in a theory of moral development …

Predicting Emotion Regulation In Early Childhood: The Impact Of Maternal Well-Being, Infant Crying, And Dyadic Mutuality, Tiffany Burkhardt

Dissertations

Learning adaptive emotion regulation skills in early childhood has been identified as fundamental to social competence, academic success, and psychological well-being. Because children learn to regulate their emotions through interactions with their caregivers, dyadic mutuality between the mother and infant may influence child emotion regulation capacity more than maternal behavior alone. To better understand the impact of maternal well-being and infant crying on the development of emotion regulation, parenting stress, maternal self-efficacy, maternal depression, and infant crying were examined with dyadic mutuality in the parent-child interaction to predict emotion regulation capacity.

A racially and socioeconomically diverse community sample of 149 …

Psychometric Evaluation Of The Neonatal Pain, Agitation, And Sedation Scale (N-Pass) Tool In Infants And Children Age One To Thirty-Six Months In The Post-Anesthesia Care Unit, Patricia A. Hummel

Dissertations

The purpose of this study was to investigate the Neonatal Pain, Agitation, and Sedation Scale (N-PASS) tool for evidence of validity and reliability in infants and children aged one to thirty-six months in the Post-Anesthesia Care Unit (PACU). This study design was a prospective, non-experimental psychometric evaluation of the N-PASS tool.

The research was conducted at a Midwestern Medical Center. The sample analyzed comprised forty subjects aged one to thirty-six months.

Data collection involved the evaluation of participants every five minutes, utilizing the N-PASS sedation subscale along with the University of Michigan Sedation Scale (UMSS) and the N-PASS pain/agitation subscale …

Adenovirus Vectors As Potent Adjuvants In Vaccine Development, Kathleen Ann Mcguire

Dissertations

Due to their ability to activate the immune system, replication-defective Adenoviruses (Ad) are potential vaccine vectors for several pathogens. The proinflammatory response to Ad contributes to the response to vaccine antigens. We found that reactive oxygen species (ROS) are an important signal in the proinflammatory response to Ad. We identified that serotype 5 adenovirus (Ad5) elicits ROS by inducing mitochondrial membrane damage, a process that is dependent on endosomal membrane rupture and Cathepsin release. This mitochondrial dysfunction contributes to NLRP3 inflammasome- and NFκB-dependent innate immune activation. The ROS-dependent inflammatory response likely contributes to the adaptive immune response by supporting DC …

The Molecular Components Of Estrogen Receptor Beta (Erβ) Signaling In Neuronal Sytems, Natasha Mott

Dissertations

With increasing life expectancy, women are now living upwards of 50 years without circulating estrogens, therefore, it is essential to investigate how the brain is changed by estrogen deprivation and also how aging influences these changes. The Women's Health Initiative (WHI) study spurred rigorous debate regarding estrogen therapy for postmenopausal women due to dichotomous effects of estrogens in menopausal and post-menopausal women. Meta-analyses of the WHI study revealed that after circulating estrogens are depleted for many years re-exposure may cause aberrant, negative health effects, indicating that there is an age-related `switch' in estrogen signaling around menopause. These age-related effects of …

The Affective Consequences Of Expressing Moral Convictions, Lisa Diana Sandberg

Dissertations

This project examined the affective consequences of expressing moral convictions to an opposing majority. It was predicted that moral conviction would function as a buffer to the common negative emotions that occur when speaking out against majority opinion (e.g., fear; Asch, 1956; Berns, et al., 2005). It was also hypothesized that moral conviction would enhance positive feelings among those who speak out (e.g., pride). Two studies were conducting using two different research paradigms. Study 1 used a normative influence paradigm modeled after Hornsey, Smith, and Begg (2007). Participants' opinions and strength of moral conviction about the target issue (torture of …

An Ethnographic Study Of Psychiatric Assistants: The Social Processes Involved In The Subordination Of Workers In A Psychiatric Hospital, Terrence Allison

Dissertations

This research examined how the structure of a modern day psychiatric institution shapes the work experiences of subordinated, non-licensed-workers. I found that that the work experiences of PAs are shaped by their institutional positions. The PA position by default is one of subjugation and servitude, and these carry meanings. PAs are positioned on the ward to service and control patients. In spite of their absolute importance to the day-to-day running of the hospital, frontline PA work is devalued institutionally. This devaluation is internalized rationally (what bureaucracies do) by PAs, who often work understaffed and underappreciated. I found that PAs lack …

Purpose And Design In Organisms And Artifacts: The Search For A Unified Philosophical Theory Of Function, Mark L. Bourgeois

Dissertations

This work examines the concept of function in both biological organisms and designed artifacts. Function is routinely attributed within both kinds of systems, yet it is unclear whether it can mean the same thing within each, and indeed whether function attribution within natural systems is legitimate at all. Function is typically understood to have teleological content; yet in true teleological causation, the end is somehow the cause of its own means. In artifacts the consciously envisioned design of an artifact is taken to be the cause of its parts and their roles. Yet in naturalistic science there is no prior …