Digital Commons Network^™

Monocyte Phenotype And Whole Blood Immune Response In Physically Active Chronic Cannabis Users And Non-Users, Jonathon K. Lisano

Dissertations

Some physically active individuals are now advocating for the use of cannabis with exercise for its potential to optimize the immune response. Yet, it remains to be seen whether the chronic use of cannabis products is linked to alterations in immune characteristics, such as monocyte phenotype and function in physically active individuals. The purpose of this cross-sectional study was to assess resting concentrations of c-reactive protein (CRP) and interleukin-6 (IL-6), monocyte phenotype and lipopolysaccharide (LPS) stimulated production of Interleukin-6 (IL-6) following pre-treated with synthetic cannabinoid 2 (CB2) receptor agonist and antagonist in physically active individuals using cannabis products at least …

Cardiovascular Changes During Dialogue, Patricia Liehr

Dissertations

This study was designed to explore the experience of listening, noting how listening was distinct from talking and from silence. There was interest in the potential for listening to engender lowered physiological arousal. Listening was defined within an interpersonal context, as one person listened to another tell a story. Clinical experience has demonstrated that persons who are listening to a clinician share a story often relax. Psychophysiological measures were used to assess the experience of listening. Cardiovascular parameters (mean arterial pressure, systolic blood pressure, diastolic blood pressure, heart rate, skin temperature) and eye contact (videotaped data) were measured during listening. …

Widowhood: The First Anniversary Spousal Bereavement-Related Stress, Coping, And Well-Being In Older Women, Mary Elizabeth Minton

Dissertations

By 2020 approximately one million individuals will experience spousal bereavement annually, primarily older women. However, little is known about the survivor's well-being around the first year death anniversary. This study examined spousal bereavement-related stress, coping, and well-being in widows 65 years and older at months 11, 12, and 13 following the death of the spouse based on Lazarus and Folkman's transactional approach and Schaefer and Moos' model of life crises and transitions. Using a prospective, longitudinal, correlational design, data were collected from 47 widows.

Bioinspired Glycopolymers: Models To Investigate The Effect Of Saccharide Structure And Concentration On Amyloid Beta Aggregation, Pradipta Kumar Das

Dissertations

Aggregation and subsequent deposition of amyloid-β (Aβ) peptide on neuronal cell membranes have been implicated as a cause of Alzheimer’s disease. Gangliosides in their clustered form seed and promote the Aβ aggregation process. However, the effects of the structure and the concentration of ganglioside saccharides on Aβ aggregation are not well understood. We investigated how the specific structure of saccharides (β-D-galactose and β-D-glucose) affect the aggregation pathways, kinetics, and the aggregated structures of Aβ via in vitro experiments. The effects of the local concentration of saccharides on the Aβ aggregation were also investigated.

To mimic the multivalent effect of the …

Biomimetic And Vascularized 3-D Liver Cancer Model, Derek Yip

Dissertations

Anti-angiogenic drugs have failed to show significant extended mortality, except when co-administered with chemotherapy drugs in clinical trials. This should be predicted by in vitro models, and yet 2D in vitro models of liver cancer co-administered with these two types of drugs show increased cell viability, contradicting clinical trials. In vitro models should mimic clinical trials in order to accurately predict drug outcomes. 2D in vitro models fail because they lack features of the cancer environment such as presence of stromal cells and a vasculature.

In order to achieve a biomimetic and vascularized in vitro model that would better recapitulate …

Lnfluences Lmpacting The Development Of Entry-Level Master's Athletic Training Programs, Jennifer E. Deranek

Dissertations

Athletic training is an allied health profession in which individuals receive education in prevention, emergency care, clinical diagnoses, therapeutic intervention and rehabilitation for injuries and medical conditions. Currently, the route for an individual to become a certified athletic trainer is to graduate from an institution accredited by the Commission on Accreditation of Athletic Training Education (CAATE). Most commonly, entry-level education for athletic trainers is gained at the 331 institutions that host a bachelor’s level professional program. However, there is a recent desire to change the educational requirements and to require a professional master’s degree. If the degree change would occur, …

Beyond Harmony: Incorporating Rhythmic Elements Of Jazz Improvisation Through Pedagogy And Curriculum, Jonathan M. Campbell

Dissertations

Jazz studies programs have become established within the realm of formal education systems and currently play an increasingly important role in the development of jazz education as well as providing means for jazz students to study improvisation. Among the array of college-level jazz courses such as jazz history, jazz arranging and composition, jazz theory, and jazz ensembles, jazz improvisation sequences are often the focal point within jazz programs. However, as previous researchers have pointed out, jazz improvisation pedagogy is almost exclusively focused on chord/scale systems and harmonic based elements of improvisation. This has led to a pedagogical model that neglects …

Psychoneuroimmunology And Chronic Pain, Sarah E. Giron

Dissertations

There is increasing evidence linking chronic pain to altered levels of central nervous system (CNS) inflammation and increased levels of perceived pain, anxiety, depression and sleep disturbance. However the inflammatory molecules responsible for physiologic and psychological components of chronic pain still warrant identification and exploration. Using central inflammation as a paramount factor in the creation and maintenance of chronic pain, this study aimed to investigate and describe the physical and psychological aspects of chronic pain associated with CNS inflammation.

Using a cross-sectional correlational design, cerebrospinal fluid (CSF) inflammatory cytokine patterns present in 8 chronic pain participants were compared to inflammatory …

Experimental Investigation On The Relationship Between Lead Exposure And Thyroids In Diabetes, Salah Sumar Ali Al-Zadjali

Dissertations

Lead exposure can cause multiple systemic toxicities, particularly affecting the hematopoietic, nervous and renal systems. However, its effects on the thyroid functions are not well elucidated and the published studies are controversial. In addition, although there are several experimental thyroid models, each one of them has its own limitations. Accordingly, in this dissertation, we investigated the possible relationship between lead exposure, thyroid functions and short-term systemic toxicity in two animal models, namely normal (non-diabetic) and diabetic animals. We also investigated the possibility of developing a hormonal thyroid model. In the non-diabetic model, Wistar rats were divided into five groups and …

Experimental Investigation On The Relationship Between Lead Exposure And Thyroids In Diabetes., Salah Sumar Ali Al-Zadjali

Dissertations

Lead exposure can cause multiple systemic toxicities, particularly affecting the hematopoietic, nervous and renal systems. However, its effects on the thyroid functions are not well elucidated and the published studies are controversial. In addition, although there are several experimental thyroid models, each one of them has its own limitations. According, in this dissertation, we investigated the possible relationship between lead exposure, thyroid functions and short-term systemic toxicity in two animal models, namely normal (non-diabetic) and diabetic animals. We also investigated the possibility of developing a hormonal thyroid model.

In the non-diabetic model, Wistar rats were divided into five groups and …

L30a Mutation Of Phospholemman Mimics Effects Of Cardiac Glycosides On Isolated Cardiomyocytes, Ryan David Himes

Dissertations

In order to determine if mutations made to phospholemman (PLM) could increase PLM binding to the Na/K-ATPase (NKA) and cause positive inotropy we performed scanning mutagenesis of the transmembrane domain of PLM and measured FRET between each mutant and NKA. We observed increased binding to NKA for several PLM mutants compared to WT, including L27A, L30A, and I32A. In isolated cardiomyocytes, overexpression of WT PLM increased the amplitude of the Ca2+ transient compared to GFP control. Ca2+ transient amplitude was further increased by L30A PLM overexpression. L30A mutation also delayed Ca2+ extrusion and increased the duration of the cardiomyocyte contraction. …

Lntegration Of Conventional Lithography And Printing Processes As A Key Enabling Technology For Printed And Flexible Sensing Systems, Binu Baby Narakathu

Dissertations

Sensors, which are used ubiquitously in a wide variety of applications, are revolutionizing the already ever-changing world we live in by providing real-time information about our surroundings. This dissertation focuses on the integration of conventional photolithography and printing processes as a key enabling technology for printed and flexible sensing systems.

Initially, an efficient opto-electrochemical sensing system, for the dual detection of heavy metal compounds was successfully developed. A novel microfluidic flow cell, with a reservoir volume of 25 μl, was designed and fabricated using acrylic. An electrochemical sensor with gold (Au) interdigitated electrodes (IDE) on a glass substrate was photolithographically …

Spectroscopy And Gas-Phase Binding Of Modified Desferrioxamine B Indicator Displacement-Based Sensors, William Scott Jones

Dissertations

A coupling reaction has been used to successfully synthesize two novel probes utilizing coumarin derivatives as fluorophores. The structure, spectroscopy, and thermodynamics of these probes were characterized and studied. These probes, along with a classical probe using a N-methyl-anthranilate fluorophore, are used to take advantage of the ability of siderophores to coordinate Fe³⁺ ions which is coupled with the attractive phosphorescence properties of lanthanide ions. The use of lanthanide metals to create an indicator displacement assays is a novel application of these probes for the detection of Fe³⁺ ions. The use of Tb³⁺ ions as the indicator …

Toward A More Perfect Definition Of Learning: Using Biomarkers To Predict And Assess Learning Performance, Samuel J. Hunt

Dissertations

This study seeks to establish groundwork for a new definition of learning based on neurogenesis capable of guiding future educational policy and practice. The purpose of the research was to: (1) produce separate increases in neurogenesis and intelligence, (2) measure the changes in neurogenesis using protein biomarkers, and (3) correlate increases in levels of the protein biomarkers with increases in intelligence. The study employed a randomized pretest-posttest, control/comparison group research design. Thirty-eight fourth- and fifth-grade students with diverse academic needs were divided into three experimental groups: chess, exercise, and combined; with an additional control group. Pre-post measures included intelligence (RSPM) …

A Methodology For Measuring Elegance In Engineered Artifacts, Stuart Feldman

Dissertations

Elegance has been asserted to be a characteristic of good design. To be useful in design, a measure of elegance that permits the comparison among designs is necessary. A measurement framework is developed for elegance of engineered artifacts using factors of usability and aesthetics. Usability is broken down into individually measurable sub-characteristics that include capability, user experience, and specific functionality. Complex attributes of aesthetics such as the absence of visual excess or a form which masks underlying complexity are quantified through characteristics that include slenderness, smoothness, and symmetry. These factors are based on the techniques and processes for integrating psychophysical …

The Rhapsody/Concerto Of Robert C. Ehle, Chia-Hua Huang

Dissertations

No abstract provided.

Energy Efficient Distributed Wearable Physiological Monitoring : Framework And Implementations, Mladen MilošEvić

Dissertations

Recent technological advances in sensors, wireless networking, mobile and cloud computing promise to fundamentally change the way health care services are delivered and used. The development and proliferation of the wearable physiological monitors enable a shift in healthcare services from centralized hospitals and medical centers to individuals and their homes. Continuous wearable health monitoring has the potential to engage users, improve wellness management, prevent disease by early detection, and assist rehabilitation and treatments. This dissertation presents a general framework and a practical implementation of computing infrastructure (both hardware and software aspects) to support mobile health and wellness monitoring applications as …

Characterization Of Chronic Glucocorticoid Exposure On Natural Killer Cell Function: A Model For Stress Induced Alterations On The Epigenetic Landscape, Justin Lee Eddy

Dissertations

Psychological stress can impact immune function through altered glucocorticoid production. Natural killer (NK) cells and their effector functions are particularly susceptible to both psychological stress as well as exogenous glucocorticoids. The purpose of this study was to assess the impact of chronic glucocorticoid (GC) exposure on NK cells to investigate the immune dysregulation associated with periods of psychological stress.

Chronic in vitro treatment of the NK92 cell line produced a dichotomous NK phenotype with enhanced proinflammatory cytokine production yet reduced NK cell lytic activity (NKCA). This dichotomy was at the individual cell level where mRNA and protein levels for proinflammatory …

Discrete Targeting Of Peptide And Non-Peptide Neurotransmitters By A Single Neuron, Daniel Kueh

Dissertations

The heart excitor (HE) motor neuron in the medicinal leech, Hirudo, releases acetylcholine (ACh) and a peptide, FMRFamide, to regulate the contractile activity of the heart tube and associated side vessels. Consistent with Dale’s Principle, it was assumed that both neurotransmitters were coreleased by all presynaptic varicosities. However, discrete peptide-positive and peptide-negative varicosities can be found consistently at particular sites of innervation on the heart tube. Dual-labeled HE neurons were produced by pressure injecting Neurobiotin into single HE neurons and applied antibodies against FMRFamide on the same preparations. Consistent with initial expectations, peptide-labeled varicosities were numerous and widely distributed …

Biofunctional Coatings Using Direct-Write Fabrication Technique For Surgical Implant Devices, Jessica L. Perkins

Dissertations

Surface modification of biomaterials is of critical value to attain desired functionality of biomedical devices and implants. Many of the conventional manufacturing methods used for the fabrication of thin film coatings lack the ability to precisely dispense biological compounds without compromising its chemical integrity. This research investigates the use of direct-write inkjet technique for the deposition of multi-layer coatings of biodegradable polymers. The Direct Write inkjet method provides selective deposition and patterning capability for depositing multi-material coatings on biomaterials for a vast array of surgical implant devices (e.g. stents for cardiovascular applications and orthopedic implants). In the first phase of …

The Effect Of Conative And Affective Traits On The Development Of Occupational Expertise Of Civilian Department Of Defense Scientists And Engineers, Eric C. Sholes

Dissertations

No abstract provided.

Abstention To Consumption: The Development Of American Vegetarianism, 1817-1917, Adam Daniel Shprintzen

Dissertations

The history of vegetarianism in the United States has long been shrouded in myth, assumption and obfuscation. Vegetarianism as a vital ideological and political movement has often been presented--even by its proponents--as a product of twentieth century modernism, reflecting a rise in ethical consumer awareness. The historical record of the nineteenth century, however, tells a very different story. The notion that dietary choices could be connected with larger social and political goals was formulated during, and changed dramatically in the nineteenth century. This dissertation charts the rise and evolution of vegetarianism in the United States from 1817 until 1917.

This …

Physician's Experiences With Death And Dying: A Phenomenological Study, Deborah Jo Corker

Dissertations

This qualitative phenomenological study attempts to gain the physician's essences, meanings and understanding surrounding death and dying. Starting with themes revealed in literature, in-depth interviews were used to ask selected physicians providing care: How does death affect them? Are physicians receiving adequate training in end-of-life care? Does the medical culture still see death as failure? How do they find meaning in end-of-life care? Using three different groups of physicians: early in career, mid-career, and retired, this research attempted to examine the phenomena of death and dying over apparent time and developmental experiences of physicians. Results: Physicians have not been adequately …

Characterization Of Tram, A Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts With The B. Fragilis Putative Coupling Protein Bcta And The B. Fragilis Relaxase Protein Bmph, Mai Thi Thanh Nguyen

Dissertations

Bacteroides spp. organisms, the most prominent bacteria of the human colon, are reservoirs of antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The B. fragilis clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling protein (CP) that powers DNA translocation, and ORF7 (TraM). In this study, we demonstrated that TraM exhibits characteristics of a required conjugal apparatus protein including 1) upregulated expression under conjugation conditions; 2) localization to the bacterial inner membrane; 3) interaction with the putative coupling protein BctA and the …

A Conceptual Integration Analysis Of Multiple Instructional Metaphors, Robert Johnson

Dissertations

The present study was conducted to investigate some of the practical implications of conceptual integration theory for educators. This research was a mixed-methods study exploring how improvements in understanding—learning—(as measured by a selectedresponse assessment) are reflected in changes in learner language (described in terms of conceptual integration patterns). After a pretest with open-response and selected-response prompts, two groups of young adult participants viewed different videos of a slightly different review lesson in genes, genetics, and heredity, the difference lying in two different metaphor conditions: the experimental group was shown a more “metaphorrich” version of the lesson using two classic metaphors …

Voting In Group Support Systems : Theory, Implementation, And Results From An Exploratory Study, Kung-E Cheng

Dissertations

Group decision making is essential in organizations. Group Support Systems (GSS) can aide groups in making decisions by providing tools and process support. GSS is especially useful for geographically or temporally distributed groups. Researchers of GSS have pointed out that convergence processes are hard to accomplish in GSS. Voting tools in GSS can be a valuable asset in alleviating the difficulty of convergence processes because voting is a concise communication of individual preferences with a well defined procedure that is accepted by group members. In addition, voting results can serve as a group memory of the convergence processes. Field observations …

Central And Peripheral Autonomic Influences : Analysis Of Cardio-Pulmonary Dynamics Using Novel Wavelet Statistical Methods, Anne Marie Petrock

Dissertations

The development and implementation of novel signal processing techniques, particularly with regard to applications in the clinical environment, is critical to bringing computer-aided diagnoses of disease to reality. One of the most confounding factors in the field of cardiac autonomic response (CAR) research is the influence of the coupling of respiratory oscillations with cardiac oscillations.

This research had three objectives. The first was the assessment of central autonomic influence over heart rate oscillations when the pulmonary system is damaged. The second was to assess the link between peripheral and central autonomic control schema by evaluating the heart rate variability (HRV) …

Molecular Analysis Of The Effects Of Atrazine On Xenopus Laevis Frogs, Ronald Edward Celestine

Dissertations

Atrazine, the most commonly used herbicide in the U.S., has been implicated as one of the factors that may be causing the worldwide decline of amphibians. First, I examined effects of atrazine on laboratory raised Xenopus laevis tadpoles, treated from 5 days post fertilization to NF stage 65/66 with a concentrationof 25 ppb of atrazine. This study showed no significant difference in body weight, length and metamorphic rate.

Second, I used DNA microarrays to examine mRNA expression levels of genes in atrazine treated and control Xenopus laevis tadpoles. I found significant changes in gene expression levels of 18 genes. These …

Time-Frequency Investigation Of Heart Rate Variability And Cardiovascular System Modeling Of Normal And Chronic Obstructive Pulmonary Disease (Copd) Subjects, Douglas Alan Newandee

Dissertations

A study has been designed to add insight to some questions that have not been fully investigated in the heart rate variability field and the cardiovascular regulation system in normal and Chronic Obstructive Pulmonary Disease (COPD) subjects. It explores the correlations between heart rate variability and cardiovascular regulation, which interact through complex multiple feedback and control loops. This work examines the coupling between heart rate (HR), respiration (RESP), and blood pressure (BP) via closed-loop system identification techniques in order to noninvasively assess the underlying physiology.

In the first part of the study, the applications of five different bilinear time-frequency representations …

A Study Of The Relationship Between Police Stress And Moral Reasoning, Coping Mechanisms, And Selected Demographic Variables, Harvey J. Burnett

Dissertations

Problem. Past research provides information on stress and its relationship to the law enforcement officer's coping mechanisms. However, there is no research on how the element of moral reasoning relates within the conceptual framework of Police stress and coping. This present study investigated the relationship between police stress and moral reasoning, coping mechanisms, and selected demographic variables among police officers.

Method. The subjects for this study were 71 full-time certified law enforcement officers in Berrien, Cass, and Van Buren counties of Michigan. A brief demographic questionnaire was utilized, followed by the Police Stress Survey (PSS), the Defining Issues Test …