Digital Commons Network^™

Computational Methods For Single-Cell And Multi-Omic Data Integration And Regulatory Network Inference, Jianlan Ren

Dissertations

Single-cell and multi-omic technologies have transformed the dissection of cellular heterogeneity and regulatory dynamics in health and disease. However, the high dimensionality, technical variability, and biological complexity of these datasets present significant challenges for integration, annotation, and interpretation. In this dissertation, a suite of computational approaches is introduced to address key problems in single-cell and multi-omic data analysis through model-based innovations and applied statistical frameworks.

First, a constrained deep learning framework for single-cell data integration, label transfer, and clustering is proposed. By incorporating biologically motivated constraints into the training process, robust performance is achieved across simulated and benchmark datasets spanning …

Optimizing Hip And Knee Assistance For Walking And Sit-To-Stand Transitions: An Intrinsic Muscle Mechanics Based Predictive Approach, Neethan Ratnakumar

Dissertations

As the global population ages, the demand for wearable assistive technologies continues to rise, driven by their potential to enhance mobility and independence in older adults. Effectively designed controllers for lower-limb exoskeletons to assist sit-to-stand (STS) and walking are crucial for delivering efficient, safe, and comfortable assistance during daily activities. Traditionally, controller optimization involves biomechanical modeling and user-specific customization. Musculoskeletal simulations play a central role in this process by providing insights into human-exoskeleton interaction dynamics, thereby informing and refining control strategies.

This work presents a simulation-driven approach for developing exoskeleton controllers for walking and STS using two distinct methods: optimal …

Turbulence And Energetic Ion Production In The Partially Magnetized Hollow Cathode Plume, Margaret Mary Mooney

Dissertations

Hall effect thrusters (HETs) are the most widely used electric propulsion (EP) device for in-space satellite propulsion. EP devices provide high efficiencies for extended mission lifetimes and low thrust for precision maneuvers. The hollow cathode is a critical HET component and undergoes extensive lifetime testing in addition to the lifetime testing of the full HET. Significant density gradients, non-Maxwellian electron behavior, and magnetic fields sufficiently strong enough to magnetize electrons in hollow cathode plumes create favorable conditions for the formation of plasma instabilities, which have an impact on cathode lifetime. Cathode qualification testing is typically conducted independently and includes a …

Therapeutic Role Of Bioactive Peptides Derived From Hemoglobin, Priya Antony Pereppadan

Dissertations

Bioactive peptides are peptide fragments derived from the proteolytic cleavage or maturation of functional proteins that have shown promising therapeutic potential in addressing major health challenges, including type 2 diabetes, hypertension, and pain management. Diabetes is characterized by high blood glucose levels due to insufficient insulin production or utilization. There are several therapeutic targets for managing diabetes, and one key target is dipeptidyl peptidase IV (DPP IV) due to its role in glucose metabolism. In hypertension, angiotensin-converting enzyme 1 (ACE1) is critical for regulating blood pressure by forming angiotensin II, and its inhibition represents an established treatment strategy. Additionally, the …

The Cellular Trafficking And Targeting Of Angiotensin-Converting-Enzyme-2 (Ace2) And Neutral-Amino-Acid-Transporter (B0at1) Variants: Implications For The Pathogenesis Of Associated Diseases And Therapy, Nesreen Fayyad Alkhofash

Dissertations

Angiotensin-converting enzyme 2 (ACE2) and the neutral amino acid transporter B0AT1, encoded by SLC6A19, are membrane proteins with pivotal roles in human physiology. ACE2 is involved in regulating blood pressure and serves as the cellular entry receptor for SARS-CoV-2, while B0AT1 facilitates amino acid absorption in the intestine. The interplay between ACE2 and B0AT1, particularly their physical interaction and co-expression in the intestine, underscores their relevance in both normal physiology and disease. Dysregulation of these proteins has been implicated in conditions such as hypertension, and Hartnup disease and they have been usurped by SARS-CoV-2 to cause COVID-19. Despite their …

Developing Chemically Defined Serum-Free Medium For Leukemic Blast And Stem Cell Expansion, Carmen Eva Finley

Dissertations

The relapse and chemotherapy resistance are the major challenges of acute myeloid leukemia (AML) treatment, driven by a rare population of leukemia stem cells (LSCs). LSCs are unique types of cells which evade standard chemotherapies and can regenerate AML post-treatment. Targeting LSC therapy remains limited, in part due to the lack of reliable in vitro AML culture systems to grow LSCs for drug discovery. This study aimed to engineer a chemically defined, serum-free 2D in vitro culture condition that supports either the expansion of leukemic blasts (LBs) or primary human LSCs by optimizing current protocols for hematopoietic stem cells (HSCs) …

The Impact Of University Engagement On Psychosocial Factors Among Division I Female Student-Athletes, Ashley A. Cook

Dissertations

Background: Female student-athletes navigate unique psychosocial challenges balancing academics and athletics, yet limited research has explored the impact of university engagement on their social and emotional well-being. This study examines the impact of university engagement on sense of social fit (SSF), nonspecific psychological distress (NPD), and loneliness among Division I female student-athletes.

Method: A cross-sectional survey was conducted among female student-athletes (n = 111) from eight sports at a mid-sized NCAA Division I university in the Southern U.S. Participants completed validated measures using the SSF scale, UCLA Loneliness scale, and Kessler-6 Psychological Distress scale. An exploratory factor analysis was performed …

Derogatory, Racist, And Discriminatory Speech (Drds) In Video Gaming, Sean Woodford

Dissertations

Video games have been examined for their effects on cognition, learning, health, and physiological arousal, yet research on social dynamics within video gaming is limited. Studies have documented the presence of derogation, racism, and discrimination in this anonymous medium. However, gamers‟ firsthand experiences are typically examined qualitatively. Thus, this study aimed to establish a quantitative baseline for the frequency of derogatory, racist, and discriminatory speech (DRDS) in gaming. DRDS frequency, sexual harassment, and hate speech measures were administered to 150 individuals from online forums and social media groups. Descriptive and inferential analyses were used to gauge which factors affected DRDS …

Exploring The Influence Of Integrating Soft Skills Training Into The Undergraduate Stem Curriculum On The Stem Identity Of Students, Candace King

Dissertations

One of the major issues that STEM graduates face is overcoming Imposter Syndrome, and there is a consensus amongst STEM graduates and job recruiters that the current higher education system is missing some elements that could better prepare students for a successful STEM career. A solution to this issue results from recent research on the importance of “soft skills.” In general, “soft skills” or non-technical skills, include communication, critical thinking, and self-motivation. These skills complement “hard skills,” or technical skills, such as skills needed for performing laboratory experiments, like pipetting and Western blotting. The lack of soft skills among recent …

Steminism: Analyzing Factors That Improve Retention Of Women In Stem, Kira Carter, Jane Kelley, Jason Vasser-Elong, Rc Patterson

Dissertations

Our co-authored research ‘Steminism: Analyzing Factors That Improve Retention for Women as STEM Majors’ analyzed factors that contributed to the retention of women in science, technology, engineering, and mathematics (STEM) programs at Missouri University of Science & Technology (Missouri S&T). Women make up half of the US population, and while careers in (STEM) are an integral part of the US economy, women are underrepresented in these career fields. The purpose of our dissertation is to address the underrepresentation of women in STEM majors. Our methodology included homogeneous sampling to collect qualitative data. More specifically, we consulted with academic advisors and …

A Descriptive Exploration Of Quality And Opportunities In Outdoor Urban Play Spaces: The Case Of Chicago Public Schools, Katherine Anne Varey

Dissertations

This study investigated the quality of children’s play spaces, both green and built, in and surrounding urban public schools in Chicago. Given children’s declining access to nature and the outdoors, especially in urban areas, the aim of the study was to both assess the current potential for high-quality outdoor experiences, as well as to uncover additional, low-cost, and low-burden opportunities that may exist for enhancing outdoor experiences. Based on a selected sample of 60 Chicago Public Schools, this mixed methods study employed quantitative ratings of play space and neighborhood quality, geographic information systems (GIS) mapping techniques to show distribution of …

Exploring Relationships Between Practicing Registered Nurses (Rns) Pharmacology Knowledge And Medication Errors, Coleen Kumar

Dissertations

Medication errors result in significant negative patient outcomes and major costs to the health care system. Registered nurses (RNs) assume a major role in the medication administration process demanding high-level knowledge and skills.

This study aimed to determine if there was a relationship between practicing RNs' pharmacology knowledge and medication error occurrence.

Utilizing New Technologies To Measure Therapy Effectiveness For Mental And Physical Health, Jonathan Ossie

Dissertations

Mental health is quickly becoming a major policy concern, with recent data reporting increasing and disproportionately worse mental health outcomes, including anxiety, depression, increased substance abuse, and elevated suicidal ideation. One specific population that is especially high risk for these issues is the military community because military conflict, deployment stressors, and combat exposure contribute to the risk of mental health problems.

Although several pharmacological approaches have been employed to combat this epidemic, their efficacy is mixed at best, which has led to novel nonpharmacological approaches. One such approach is Operation Surf, a nonprofit that provides nature-based programs advocating the restorative …

Investigating Smooth Muscle Myosin Dynamics And Assembly In Physiology And Pathology, Maggie Bennett

Dissertations

Vascular smooth muscle cells (SMCs) surround many blood vessels throughout the body,where they dynamically alter vessel diameter to regulate blood pressure, provide structural integrity, and absorb shock on a beat-to-beat timescale. As smooth muscle function fails, profound vascular disease ensues, often with tragic results- even death. Smooth muscle myosin 2 (SM2) is the dominant motor protein that actuates contractility and allows SMCs to perform these vital functions. To function, SM2 monomers dynamically assemble into filaments, which upon SMC activation, associate with filamentous actin to drive contractility. Despite the critical contribution of SM2 to SMC function, foundational aspects of SM2 assembly …

A Novel Biomaterial Platform For Cardiac Tissue Engineering, Andrew House

Dissertations

Heart disease is one of the leading causes of death in the world. With a growing demand for in vitro cardiac models that can recapitulate the complex physiology of the cardiac tissue, there is a desire to create novel therapies and treatments. However, due to a lack of fundamental understanding of the heart's inflammatory response to myocardial infarction, the development of new therapies is both time consuming and costly. In this work, a novel approach to fabricating a dynamic hydrogel cardiac platform is reported. This platform better mimics the dynamic changes occurring in the native cardiac tissue microenvironment during cardiac …

Impacts Of Intermediate And Long-Term Vitamin D Deficiency On The Murine Gastric Epithelial Cells And Acid Secretion, Shaima Raji Sirajudeen

Dissertations

The biological functions of vitamin D (VD) are diverse. It influences immunological response, cell proliferation and differentiation, and calcium homeostasis. However, over one billion people globally are VD deficient. The VD receptors, VDR and PDIA3, mediate the biological activities of VD. Studies on the biological roles of VD are numerous but very little on the stomach. The stomach is divided into three main parts – forestomach, corpus and antrum. Gastric glands, which make up the functional units of the stomach, are made up of numerous cell lineages and are located in the corpus and antrum, which are the glandular parts …

Structural And Transient Kinetic Analysis On Mechanism-Based Inactivators Of Human Ornithine Aminotransferase, Arseniy Butrin

Dissertations

HCC, Hepatocellular carcinoma, MBI, Mechanism-based inactivator, OAT, Ornithine Aminotransferase

The Roles Of A Probiotic Exopolysaccharide In Breast Cancer, Mai Rachel Nguyen

Dissertations

breast cancer, exopolysaccharide, probiotic

Development Of Smart Wearable Sensory Systems On Flexible And Fabric Platforms For Health Monitoring Applications, Simin Masihi

Dissertations

Wearable sensing technology has recently received an increased attention in both research and commercialization phases and has moved from a vision of science fiction to reality with a wide variety of applications. This incredible growth can be attributed to several factors, such as affordability and ergonomics provided by advances in flexible electronics. The other factors that realized wearable sensing technology include a critical need for more accessible health care services experienced during the recent COVID pandemic, integration to the Internet of Things (IoT), and a growing consumer desire for health awareness. This dissertation presents the fulfillment of three main projects …

Are Natural Killer Cells Novel Mediators Of The Effects Of Cannabidiol On Mental Health, Sleep Quantity, Sleep Quality, And Immune Function?, Jacob Norbert Kisiolek

Dissertations

Cannabidiol (CBD) has increased in popularity since the United States Farm Bill legalized hemp production in 2018 and is now projected to become a $24.4 billion dollar industry by 2025. Since its legalization, CBD is thought to be an antiepileptic, anxiolytic, and antipsychotic, as well as an agent to improve mental health, quality of life, aspects of sleep and immune function. However, many of these claims lack scientific evidence. The purpose of this randomized, double blind, placebo-controlled trial was to determine the effects of an 8-week CBD intervention on measures of mental health, sleep quantity, sleep quality, and natural killer …

Clinical Nursing Faculty Perceptions Of Undergraduate Baccalaureate Nursing Student Stress In The Clinical Environment, Catherine A. Stubin

Dissertations

High stress levels are experienced by student nurses throughout their professional education, with the chaotic ever-changing clinical environment being a predominant source of stress. Clinical nursing faculty have a critical role to recognize the stress their students experience in the clinical environment and understand its significance.

A qualitative descriptive design was used to provide a rich description of clinical nursing faculty perceptions of undergraduate baccalaureate nursing student clinical stress. A purposive sample of 14 clinical nursing faculty with at least 4 semesters of clinical nursing faculty experience from accredited, generic baccalaureate nursing programs in the Northeast region of the United …

A Study Of Relationships Among Three Assessment Methods For Nurse Anesthetists, Nicholas W. Gabriel

Dissertations

The current measures of competency for nurse anesthetists for recertification are continuing education units for each biennial recertification cycle, and records of current practice and state licensure. Recently, the National Board of Certification and Recertification of Nurse Anesthetists (NBCRNA) adopted new standards that include a written examination every 8 years, an increase of 40 continuing education units per year, and completion of four core competency modules each four-year recertification cycle. However, little is known about the validity of these competency measures.

The purpose of this study was to determine relationships between written examination scores, self-assessment scores, and performance scores in …

The Role Of Berberine, A Clinically Relevant Plant Derived Alkaloid, In T Cell-Mediated Immunosuppression, Alexandra Adorno Vita

Dissertations

The primary focus of this research was to determine the effect of berberine (BBR), a clinically relevant plant-derived alkaloid, on follicular T helper (Tfh) cell activation and differentiation, with implications for the generation of T cell-dependent humoral responses. This research revealed a novel function of BBR as a suppressor of Tfh cell proliferation in secondary lymphoid organs of BBR-treated mice that underwent immunization for arthritis induction in a collagen-induced arthritis (CIA) mouse model. Tfh cells provide signaling to B cells that is crucial for the generation of T cell-dependent humoral responses, such as affinity maturation, isotype class switching, and the …

The State Of Missed Nursing Care In A Telemetry Nursing Unit: A Descriptive Case Study, Ann Marie Svolos

Dissertations

Missed nursing care in the acute hospital setting is care left undone. Omitted, missing and even rationed care, are not new concepts in nursing practice. However, in 2006 Dr. Beatrice Kalisch formally identified the type of care that is regularly missed in acute general hospital units and the reasons missed nursing care occurs. Missed nursing care is a problem for nursing and healthcare because it may lead to negative patient and staff outcomes which may result in harm. The purpose of the research was to understand missed nursing care from a telemetry nurse's perspective to learn how it continues to …

The Effectiveness Of Preoperative Preparation Program In Decreasing Parental Preoperative Anxiety, Thao L. Tran

Dissertations

This project aimed to examine the effectiveness of a preoperative preparation program (PPP) in decreasing parental anxiety. When children needed anesthesia to get outpatient procedures completed, the accompanying parents received verbal education about the process on the day of the procedure and were assisted by a support person during and after the child's induction anesthesia. Parental anxiety was measured using the State-Trait Anxiety Inventory (STAI) tool before preoperative preparation and after the induction of anesthesia to assess whether the PPP improved parents' anxiety levels. The parents' decreased anxiety score in post-intervention would suggest that the PPP can be effectively and …

F Plasmids In Escherichia Coli Decrease Permissivity To Coliphage, Cesar Eber Montelongo Hernandez

Dissertations

The urinary tract contains a community of bacteria called the urinary microbiota (urobiota) thatmay be relevant to health; the genomic component of the urobiota is the urinary microbiome (urobiome). Urinary bacteria have been associated with both asymptomatic states and disease conditions, such as urinary tract infection (UTI), overactive bladder (OAB), and urge urinary incontinence (UUI). Some bacteria, such as E. coli, are considered urinary pathogens (uropathogens) but also can be commensals. Bacteriophage (phage) are ubiquitous in nature and likely shape bacterial populations in every niche; thus, phage may be one factor that modulates the urobiota. Phages have a specific host …

Fostering Resilience: A Pilot Study For Mindful Yoga As An Intervention For Adolescents Exposed To Chronic Adversity, Michele Pinellas

Dissertations

The effects of ongoing stress can cause chronic affect dysregulation, destructive behavior against self and others, learning disabilities, dissociative problems, somatization, and distortions in concepts about self and others (Bessel van der Kolk, 1994, p. 259). There may be particular benefits for youth who live in urban, underserved populations as they have experienced social challenges such as poverty, violence, drugs, racism, and immigration. Given the historical tendency of youth to express their emotions externally as a coping strategy, there is rationale to support a physically based treatment, like yoga, as an intervention for this population (Beltran et al., 2016). This …

Participation In Sporting Activities As A Mechanism For Enhancing Science Instruction, Erin Roades

Dissertations

ABSTRACT

After students have taken years of science classes, they still believe science content is disconnected to the “real” world or to their lives. Teaching without attention to relevance results in decreased motivation for learning and negative attitudes toward science. An instructional method incorporating physical, sport-related activities was used to develop relevance and enhance achievement in science compared to typical/traditional science instructional methods. In the typical method, science lessons consisted of front-loading science content through direct instruction followed by a laboratory activity to confirm lesson content. The experimental teaching strategy used was “Relevance Integration for Teaching Science using Sports Exploration” …

Self-Care Domains, Benefits Of Self-Care In Graduate-Level Training Programs, And Ways To Incorporate Self-Care Education And Practices In Graduate-Level Training Programs, Krystel Segovia

Dissertations

A literature review was conducted to examine the concept of self-care as a proactive means to manage the stressors of life, enhance professional competency, and live a well-balanced lifestyle, particularly for graduate-level psychology students. Different levels of distress, including stress, compassion fatigue, and burnout, were researched and discussed. The various domains of self-care were discussed as were the benefits of engaging in regular self-care practices. Finally, various ways to incorporate self-care into already established graduate-level training programs at universities and practicum training sites were discussed to enhance the training experience as well as mitigate burnout in the novel clinician.

Effect Of Sedative Music And Scheduled Rest On Anxiety, Pain, And Myocardial Oxygen Demand During Chair Rest In Adult Postoperative Open-Heart Patients, Jo A. Voss

Dissertations

Open-heart surgery patients in ICU report anxiety and pain with chair rest despite administration of opioid analgesics. In addition, chair rest increases myocardial oxygen demand (MVO2). Non-pharmacological, complementary methods (such as sedative music and scheduled rest) used to reduce anxiety, pain, and MVO2 during chair rest warranted further investigation. The conceptual framework was based on the transactional model of stress and coping. A 3-group pretest-posttest repeated measures experimental design examined the effect of music and rest on anxiety, pain sensation, and pain distress and MVO2 during chair rest in adult postoperative open-heart surgery patients.