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Identification And Characterization Of Genes Involved In Antioxidant Traits In Local Thai Rice (Oryza Sativa L.), Putut Rakhmad Purnama
Identification And Characterization Of Genes Involved In Antioxidant Traits In Local Thai Rice (Oryza Sativa L.), Putut Rakhmad Purnama
Chulalongkorn University Theses and Dissertations (Chula ETD)
Developing rice (Oryza sativa L) cultivars with high antioxidant activities have become increasingly important since they have nutritional advantages for human health. Hence, the researchers must precisely elucidate the genetic bases controlling antioxidant-related traits in rice grains. In this study, we aimed to identify the genes involved in antioxidant traits in local Thai rice. The first strategy was conducting a genome-wide association analysis (GWAS) using 233 accessions of local Thai rice. A total of 119,541 single nucleotide polymorphic (SNP) markers was used as genotype data. In contrast, the five antioxidant assays, ABTS, DPPH, FRAP, TFC, and TFC, were used as …
Metabolomic Analysis Of Lungs From Breast Cancer Metastatic Mice Models Treated With Thai Traditional Medical Formulary, Kotchanipa Rukruam
Metabolomic Analysis Of Lungs From Breast Cancer Metastatic Mice Models Treated With Thai Traditional Medical Formulary, Kotchanipa Rukruam
Chulalongkorn University Theses and Dissertations (Chula ETD)
Triple-negative breast cancer (TNBC) is a highly invasive subtype, leading to lung metastasis in 40% of patients with TNBC metastasis. Lung metastases significantly reduce median survival compared to other breast cancer types. Hence, there are efforts to investigate TNBC treatments, including modern and alternative therapies. The Thai traditional medical formulary (TTMF) is broadly used as an alternative treatment for Thai cancer patients. However, there are no studies on metabolic changes after treatment in advanced TNBC. Additionally, obtaining metabolites from biological samples is challenging due to the lack of a universal protocol for metabolite extraction. Consequently, this thesis aimed to first …
Contamination Of Antimicrobial-Resistant Escherichia Coli, Salmonella Spp., Enterococcus Faecalis, And Enterococcus Faecium In Commercial Raw Meat-Based Diets For Cats And Dogs, Rusmin Indra
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to investigate the contamination of third-generation cephalosporin (3GC)-resistant Escherichia coli, Salmonella spp., Enterococcus faecalis, and E. faecium in commercial raw meat-based diets (RMBDs) for cats and dogs in Thailand. Fifty RMBD products from ten brands were included for total plate count and bacterial isolation. Antimicrobial susceptibility testing was conducted for the targeted bacteria. Extended-spectrum β-lactamase (ESBL) production and the presence of blaCTX-M and mcr genes were detected. Additionally, E. coli phylogroups and Salmonella serovars were identified. The RMBD samples were contaminated by 3GC-resistant E. coli (80.0%), Salmonella spp. (70.0%), E. faecalis (100%), and E. faecium (84.0%). All …
National Survey Of Human Epigenetic Study In Healthy Aged Population In Four Different Regions Of Thailand, Siwaporn Nilyai
National Survey Of Human Epigenetic Study In Healthy Aged Population In Four Different Regions Of Thailand, Siwaporn Nilyai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Healthy ageing has become a goal to help older people maintain good-quality longevity. Researchers have found that epigenetic, environmental, and behavioural lifestyle factors influence the variation of lifespan and healthspan. This study aims to evaluate the age-associated epigenetic biomarkers among older people living in four major regions of Thailand, along with their association with lifestyle and sociodemographic characteristics. 214 participants passed the criteria and enrolled from the southern, northeastern, central, and northern areas of Thailand. Sociodemographic characteristics were recorded through interviews, and blood samples were collected to evaluate blood chemistry and epigenetic markers. SIRT1 and HMGB1 proteins in plasma were …
Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit
Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Volatile organic compounds (VOCs) are the metabolites of microorganisms. VOCs are low molecular weight and easily evaporate at room temperature, all odorants are VOC. VOCs are different depending on health status and reproductive status. The objective of this study was to profile the VOCs in bitches during estrous cycle, and to compare VOCs between stages of estrous cycle and between pre-ovulatory, ovulation and post-ovulatory periods. A total of 23 normal, healthy bitches requested for ovulation timing as part of the routine breeding management were used in this study. All animals had a previous history of normal estrous cycle without any …
Inhibition Of Chondrocyte Apoptosis Via Fas/Fasl System In Knee Osteoarthritis Patients, Ousakorn Kamenkit
Inhibition Of Chondrocyte Apoptosis Via Fas/Fasl System In Knee Osteoarthritis Patients, Ousakorn Kamenkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Osteoarthritis (OA) is a degenerative rheumatic disease characterized by articular cartilage degeneration. This articular cartilage degradation is positively correlated with chondrocyte apoptosis. The Fas/Fas ligand (FasL) system is part of the extrinsic apoptosis pathway that regulates cell death through the binding of Fas on target cells to FasL on effector cells. Although Fas+ chondrocytes have been identified and the Fas/FasL system has been implicated in this apoptotic process, the apoptosis through chondrocyte-to-FasL+ cell interaction remains unclear. Therefore, this study aims to investigate Fas/FasL-mediated chondrocyte apoptosis focusing on cell-to-cell interactions. In our study, we conducted ex vivo experiments using flow cytometry …
Genome Characterization And Significant Gene Annotation Of Mycobacteria Based On Data From High-Throughput Dna Sequencing Technology, Ajala Prommi
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mycobacteria encompass a variety of species, including pathogenic strains such as Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM). These organisms are responsible for significant health challenges globally and in Thailand, particularly due to tuberculosis (TB) and its drug-resistant forms. This dissertation aims to characterize the whole genome of various Mycobacterium species and annotate significant genes utilizing high-throughput DNA sequencing technology. Key objectives include investigating the mutations in Mycobacterium tuberculosis responsible for resistance to primary anti-TB drugs, specifically isoniazid, and analyzing the resistome of Mycobacterium abscessus, a rapidly growing NTM, isolated from patients in Thailand. The study also seeks to …
Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem
Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem
Chulalongkorn University Theses and Dissertations (Chula ETD)
Animal welfare is a rising topic in the swine industry, resulting in the adoption of alternative housing systems. However, the knowledge of management practices to mitigate the deleterious effects of these systems on reproductive performance in tropical environments is limited. This thesis aims to determine the impacts of alternative housing systems on the reproductive performance of sows and gilts, comprising 4 parts. i) To compare reproductive performance among conventional crates (CC), group housing gestation pen with temporary farrowing crate (PC), and group housing gestation pen and free-farrowing pen (PP). On average, the farrowing rate of sows in the CC group …
Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit
Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit
Chulalongkorn University Theses and Dissertations (Chula ETD)
A new clinical chemistry analyzer using surface plasmon field-enhanced fluorescence spectroscopy (SPFS) has been introduced into veterinary diagnostic laboratories as a point-of-care unit, enabling rapid turnaround times for both diagnosis and follow-up. Consequently, the test results must be accurate, precise, and reliable. This research aims to evaluate the method performance of the analyzer. The evaluation was conducted according to Clinical Laboratory Improvement Amendments (CLIA) guidelines using method validation. The performance evaluation included studies on imprecision (within-day and between-day), method comparison, recovery tests, and linearity for determining progesterone concentration. The method validation results showed good and acceptable precision. Serum spiking concentrations …
Metagenomic Analysis Using Oxford Nanopore For Fungal Identification In Hemoculture Sample, Nattapong Langsiri
Metagenomic Analysis Using Oxford Nanopore For Fungal Identification In Hemoculture Sample, Nattapong Langsiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Currently, with advancements in medical technology, the mortality rate from fungal infections has also increased, particularly since the late 20th century. The incidence of invasive fungal infections, especially bloodstream fungal infections, is rising. However, laboratory diagnosis of fungi from clinical specimens still faces significant limitations. Diagnosing fungi based on morphological characteristics to identify species level can take weeks, which is not aligned with the severity of the disease. Current molecular biology techniques also have limitations, such as PCR success rates and mixed infections. With significant advancements in sequencing technology leading to third-generation sequencing, there are notable benefits. Third-generation sequencing, or …
การพัฒนาตัวชี้วัดเพื่อประเมินคุณภาพพื้นที่สีเขียวในประเทศไทย, ธนโชค วานิชผดุงธรรม
การพัฒนาตัวชี้วัดเพื่อประเมินคุณภาพพื้นที่สีเขียวในประเทศไทย, ธนโชค วานิชผดุงธรรม
Chulalongkorn University Theses and Dissertations (Chula ETD)
การศึกษาในครั้งนี้มีวัตถุประสงค์เพื่อพัฒนาตัวชี้วัดสำหรับประเมินคุณภาพพื้นที่สีเขียวและทดสอบประสิทธิภาพการใช้งาน การศึกษาทำโดย 1) กำหนดวัตถุประสงค์การใช้งานตัวชี้วัดและผู้ที่มีส่วนเกี่ยวข้อง, 2) รวบรวมตัวชี้วัดที่เกี่ยวข้อง, 3) วิเคราะห์และเลือกตัวชี้วัดที่เหมาะสม, 4) จัดทำเป็นแบบประเมิน และ 5) ทดสอบกับพื้นที่สีเขียวตัวอย่างจำนวน 15 แห่ง ได้แก่ ป่าธรรมชาติ/ป่าชุมชน 3 แห่ง สวนสาธารณะ 4 แห่ง พื้นที่ส่วนบุคคล 5 แห่ง และหลังคาเขียว 3 แห่ง ผลการศึกษาได้ตัวชี้วัดคุณภาพพื้นที่สีเขียวแบ่งเป็น 4 ด้าน ได้แก่ สภาพแวดล้อม ความหลากหลายทางชีวภาพ บริการของระบบนิเวศ และการจัดการในพื้นที่ รวม 124 ตัวชี้วัด 370 คะแนน ผลการใช้ตัวชี้วัดพบว่าคะแนนคุณภาพพื้นที่สีเขียวอยู่ในช่วงระหว่าง 296-152 คะแนน ซึ่งเป็นผลจากวัตถุประสงค์การใช้งานและลักษณะพื้นที่ที่มีความหลากหลาย คะแนนแต่ละด้านของพื้นที่สีเขียวแต่ละประเภทส่วนใหญ่มีความสัมพันธ์กับลักษณะพื้นที่ การดูแลและการใช้งานของมนุษย์ ในส่วนของการใช้งานพบว่าผู้ใช้งานมีความพึงพอใจแบบประเมินที่สร้างขึ้นโดยภาพรวมในระดับปานกลางถึงดี (3.73 ± 1.08 คะแนน) โดยเป็นผลจากการออกแบบการประเมินในรูปแบบรวดเร็ว มีการอำนวยความสะดวกในการบันทึกข้อมูลในโปรแกรม Microsoft Excel ใช้เวลาและกำลังคนในการประเมินต่ำ แต่แบบประเมินยังต้องพัฒนาคำอธิบายรายละเอียดและขั้นตอนใช้งานรวมถึงเกณฑ์การให้คะแนนเพิ่มเติม นอกจากนี้ ควรเพิ่มจำนวนพื้นที่สีเขียวที่ประเมินให้มากขึ้นเพื่อทดสอบและปรับปรุงประสิทธิภาพตัวชี้วัด ตลอดจนส่งเสริมให้ผู้มีส่วนเกี่ยวข้องกลุ่มต่าง ๆ นำไปใช้ประโยชน์เพื่อพัฒนาคุณภาพพื้นที่สีเขียวของประเทศต่อไป
Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa
Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Influenza A viruses (IAVs) can infect birds and a wide variety of mammals. Live bird markets (LBMs) and free-grazing ducks (FGDs) are the important reservoirs of influenza A viruses in Thailand. This study aims to gain the overall information of IAVs circulated in LBMs in Bangkok and in FGD flocks in Thailand. IAVs surveillance in LBM in Bangkok was conducted to identified the IAVs in LBM during 2019-2020. The IAV-H9N2 (n=8) and IAV-H9Nx (n=2) were isolated from 1,178 swab samples. The IAVs of the previous CU-EIDAs’ LBM surveillance programs during 2014-2018 were included for overall genetic analysis. Total 6 subtypes …
Molecular Investigation And Genetic Characterization Of Canine Astrovirus And Canine Kobuvirus With Associated Pathology In Domestic Dogs In Vietnam And Thailand, Tin Van Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine astrovirus (CaAstV) and canine kobuvirus (CaKoV) are recently recognized pathogens associated with canine gastrointestinal disease (GID), though their exact role remains poorly understood. In both Vietnam and Thailand, these viruses were detected in dogs with and without diarrhea, particularly high frequency in puppies under 6 months old. Co-infection between CaAstV and CaKoV, as well as with other common canine enteric viruses (canine parvovirus, canine enteric coronavirus, canine distemper virus), were also confirmed. Complete coding sequences (CDS) of 7 CaAstV strains and 9 CaKoV strains from Vietnam, and 2 CaAstV strains and 4 CaKoV strains from Thailand were successfully identified. …
Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit
Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Overpopulation of companion animals, particularly dogs and cats, is a global concern, necessitating effective population control policies. This research investigates the attitudes of veterinarians and pet owners in Thailand regarding dog and cat surgical sterilization and the use of progestins as non-surgical contraception. The study also assesses the effects of intratesticular administration of alpha-mangostin encapsulated in nanostructured lipid carriers (AM-NLC) in male cats, and adverse effects. Furthermore, a novel formulation of AM-NLC for intratesticular administration is also developed. Surveys reveal that decisions on sterilization are influenced by species, sex, COVID-19 impacts, and demographic factors. And anesthesia risks remain a primary …
Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat
Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat
Chulalongkorn University Theses and Dissertations (Chula ETD)
The importance and rationale of this thesis were to address the critical knowledge gap in equine endometrosis by developing a comprehensive 3-dimensional (3D) in vitro model of equine endometrial tissue using advanced technology like 3D bioprinting. Study 1 aimed to develop an in vitro model for studying equine endometrial function by isolating and characterizing equine endometrial epithelial cells (eECs) and endometrial mesenchymal stem cells (eMSCs) using a modified tissue explantation outgrowth technique. Both cell types were successfully cultured in specific medium conditions, maintaining their characteristic features. Notably, eECs retained their function in fetal bovine serum (FBS) and KnockOut™ serum replacement …
Value-Added Natural Fiber As A Biocompatible Substrate For Chemical Sensor Applications, Wisarttra Phamonpon
Value-Added Natural Fiber As A Biocompatible Substrate For Chemical Sensor Applications, Wisarttra Phamonpon
Chulalongkorn University Theses and Dissertations (Chula ETD)
This dissertation focuses on the improvement and value added of natural fiber extracted from the UHT milk carton waste, and raw silk cocoon to fabricate the biocompatible substrate for chemical sensors, principally in colorimetric and electrochemical sensors, which is allocated in 3 main parts. Initially, the background studying of the surface modification and the function of natural fiber based chemical sensor for food quality control and clinical diagnosis will be explained in a review article. Subsequently, the development of recycled cellulose from UHT milk carton waste as a novel substrate of dual lactate sensor for milk spoilage indication will be …
A Comprehensive Study Of African Horse Sickness Outbreak In The Northeastern Region Of Thailand: Outbreak Investigation, Genetic Characterization, And Longitudinal Serological Monitoring, Napawan Bunpapong
Chulalongkorn University Theses and Dissertations (Chula ETD)
African horse sickness (AHS) results from infection with the African horse sickness virus (AHSV). The AHSV is a non-contagious and belongs to genus Orbivirus, family Reoviridae and primarily by Culicoides midges. AHS caused the devastating infection in equine population, including horses, ponies, European donkeys, and mules. AHS maintains its occurrence in southern African countries, it may possess threatening impact to the naïve regions such as Asia or Southeast Asia. The objectives of this study are 1) To investigate the emerging AHS outbreak in the Northeastern region of Thailand, 2) To characterize emerged AHSV in Thailand and 3) To monitor serological …
Effects Of Self-Adhesive Resin Cements On Human Gingival Fibroblasts, Kittipat Termteerapornpimol
Effects Of Self-Adhesive Resin Cements On Human Gingival Fibroblasts, Kittipat Termteerapornpimol
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objectives: To investigate the response of primary human gingival fibroblasts (HGFs) to self-adhesive resin cement (SARC) extracts. Methods: Two types of SARCs, RelyXTM U200 (RX) and Maxcem EliteTM Chroma (MC), were polymerized using various curing methods (light-cured, light-cured through 1- or 2-mm zirconia, and self-cured) (n=9/group). HGFs were exposed to cement extracts at different concentrations. Treated HGFs were assessed for cell viability, vitality, wound healing, and gene expression at different time points. Results: Dual-cured MC left self-cured and MC cured through 2 mm zirconia at high concentration significantly decreased the viability of HGFs. Delayed wound healing was observed in MC …
ผลการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้ที่มีต่อสมรรถนะการเขียนของนักเรียนมัธยมศึกษาตอนปลาย, ชุติมา หนูเกื้อ
ผลการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้ที่มีต่อสมรรถนะการเขียนของนักเรียนมัธยมศึกษาตอนปลาย, ชุติมา หนูเกื้อ
Chulalongkorn University Theses and Dissertations (Chula ETD)
การวิจัยนี้มีวัตถุประสงค์เพื่อ 1) เปรียบเทียบสมรรถนะการเขียนของนักเรียนที่ได้รับการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้ก่อนทดลองและหลังการทดลอง 2) วิเคราะห์การเปลี่ยนแปลงสมรรถนะการเขียนของนักเรียนมัธยมศึกษาตอนปลายระหว่างที่ได้รับการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้ ตัวอย่างวิจัย ได้แก่ นักเรียนมัธยมศึกษาปีที่ 5 จำนวน 40 คน ของโรงเรียนมัธยมศึกษาขนาดใหญ่พิเศษแห่งหนึ่งในสังกัดสำนักงานเขตพื้นที่การศึกษามัธยมศึกษานครศรีธรรมราช เครื่องมือที่ใช้ประกอบด้วย แบบวัดความสามารถในการเขียน แบบวัดเจตคติต่อการเขียน แบบบันทึกสมรรถนะการเขียนรายบุคคล แบบบันทึกการเรียนของนักเรียน และแผนการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้ วิเคราะห์ข้อมูลโดยใช้ค่าเฉลี่ยเลขคณิต ส่วนเบี่ยงเบนมาตรฐาน และการทดสอบค่าที ผลการวิจัย พบว่า 1) นักเรียนมีคะแนนเฉลี่ยสมรรถนะการเขียนทั้งด้านความรู้ ด้านทักษะ และด้านเจตคติหลังการจัดการเรียนการสอนสูงกว่าก่อนจัดการเรียนการสอน อย่างมีนัยสำคัญทางสถิติที่ระดับ .05 2) ผลการวิเคราะห์ข้อมูลเชิงคุณภาพเพื่อศึกษาการเปลี่ยนแปลงสมรรถนะการเขียนของนักเรียนที่ได้รับการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้ จำแนกตามองค์ประกอบ พบว่า นักเรียนที่ได้รับการจัดการเรียนการสอนแบบอิงบริบทร่วมกับเครื่องมือดิจิทัลเพื่อการเรียนรู้มีสมรรถนะการเขียนครั้งหลังดีขึ้นกว่าครั้งก่อนหน้าทุกองค์ประกอบ
Efficacy Of Nanogel From Grafted Hyaluronic Acid Saliva Substitutes In The Treatment Of Dry Mouth, Nongnapat Eakpunyakul
Efficacy Of Nanogel From Grafted Hyaluronic Acid Saliva Substitutes In The Treatment Of Dry Mouth, Nongnapat Eakpunyakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective of this study was to evaluate the efficacy of HA-pNIPAM spray or Hy-N spray in treating dry mouth and to evaluate patient satisfaction with Hy-N spray. This study was a randomized double-blind crossover study conducted on patients with dry mouth. They were given three saliva substitutes: Hy-N, placebo, and Biotène spray, which were randomly allocated in a different order. Each spray was used for 1 week, followed by a 3-day wash-out period. The instruction was to use the sprays four times a day. A total of twenty-nine patients completed the study. A significant increase in unstimulated whole saliva …
Comparing Medium To Long-Term Esthetic, Clinical And Patient-Reported Outcomes Between Freehand And Computer-Assisted Dental Implant Placement, Sun Heng
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective: The aim of the study was to compare medium to long-term clinical and patient reported outcomes between implants placed with Computer-assisted Implant Surgery (CAIS) and freehand protocols. Materials and Methods: Thirty dental implants in the anterior maxillary region with an average of 4 years after loading were assessed by means of Pink Esthetic Scores (PES), Marginal Bone Level (MBL), clinical and patient reported outcomes. Results: CAIS significantly outperformed freehand placement with regards to PES scores (p=0.011). Likewise, implants placed with CAIS showed significantly higher marginal bone levels (p<0.001). Bleeding on Probing, Probing depth and prevalence of mucositis did not differ between the groups, while no peri-implantitis was diagnosed. Conclusions: The use of CAIS lead to superior outcomes in terms of esthetics and marginal bone levels for implants placed in the aesthetic zone as observed in medium to long-term follow-up. No difference was however observed with regards to peri-implant mucosa inflammation.
Comparison Of Learning Curve In Novice And Experienced Operators Between Anterior And Posterior Implant Placement Using Dynamic Computer Assisted-Surgical Implant Placement: Randomized Controlled Trials, Pitsinee Kundaechanont
Comparison Of Learning Curve In Novice And Experienced Operators Between Anterior And Posterior Implant Placement Using Dynamic Computer Assisted-Surgical Implant Placement: Randomized Controlled Trials, Pitsinee Kundaechanont
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objectives: To observe the equivalence of learning curve between anterior and posterior implant placement and between novice and experienced operators using dynamic computer assisted-surgical implant placement (CAIS) with type-III bone model simulated maxilla. Materials and Methods: Eight participants, including four novices and four experienced operators, were randomly assigned in blocks to either anterior or posterior placement group. Prior to the experiment, participants underwent video-training. Each participant performed four single implant placements per session with a 7±1 days wash-out period, totaling five attempts. The operating time, coronal, depth, and angle deviations in each attempt were recorded and analyzed by a blind …
Analysis Of Gene Expression And Histology Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 In Rat Femur, Duangporn Yongyosrungrueng
Analysis Of Gene Expression And Histology Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 In Rat Femur, Duangporn Yongyosrungrueng
Chulalongkorn University Theses and Dissertations (Chula ETD)
The quality of bone healing is an important factor in dental implant treatment. Currently, there is only limited research on using mRNA encoding BMP-2 to improve the quality and quantity of bone. This study aimed to evaluate the effectiveness of bone healing in rat femur defect after delivery of dPBS, rhBMP-2 and mRNA-encoding BMP-2 (BMP-2 mRNA-LNP). Histology approach was used to measure the quantity of newly formed bone. RT-qPCR was used to study gene expression levels of BMP-2, OCN, BSP and TNF-α. Subsequently, RNA-Seq analysis was performed at six weeks post-operation. Histological study revealed that BMP-2 mRNA-LNP group had significantly …
The Effect Of Different Self-Adhesive Resin Cements On Shear Bond Strength Of Hybrid Ceramic And Dentin, Rattawich Hoonsuwan
The Effect Of Different Self-Adhesive Resin Cements On Shear Bond Strength Of Hybrid Ceramic And Dentin, Rattawich Hoonsuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
ActivaTM bioactive is the first developed of dual cured, self-adhesive resin cement. For this in vitro study was to compare and evaluate the three differentsshear bond strength among ActivaTM bioactive, Maxcem EliteTM and RelyXTMU200 cement, when used as adhesive between CAD-CAM hybrid ceramic; Vita EnamicTM and dentin surface. The Vita EnamicTM block was designed and fabricated into forty-two discs shaped with CAD software. Forty-two extracted third maxillary molars was also collected. The all teeth were free of dental caries, restoration and cracked line were mounted vertically in the epoxy resin with exposedthe occlusal surface. Dentin surfaces were flatted surface by …
Micro-Computed Tomography And Mechanical Test Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 With Implant Placement In Rat Femur, Kanoksiri Jriyasetapong
Micro-Computed Tomography And Mechanical Test Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 With Implant Placement In Rat Femur, Kanoksiri Jriyasetapong
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research evaluates the effectiveness of a modified mRNA encoding bone morphogenetic protein-2 (m1Ψ-BMP-2 mRNA) with N1-methylpseudouridine modifications on bone regeneration and integration after implantation into rat femur defects. In the study, twelve femurs from six Sprague-Dawley rats underwent titanium wire insertation. These femur defects were then filled with either 5 μg of m1Ψ-BMP-2 mRNA, 15 μg of m1Ψ-BMP-2 mRNA, or Dulbecco's phosphate-buffered saline (dPBS) in a randomized manner (N=4 each group) Six weeks post-implantation, the rats were euthanized for evaluation using micro-computed tomography and a mechanical push-out test. The Micro-CT analysis focused on several parameters including total bone volume, …
Effect Of Different Implant Angulations And Insertion-Removal Cycles On The Retentive Force Of Locator And Locator R-Tx Attachments, Jidapha Tangtrongmit
Effect Of Different Implant Angulations And Insertion-Removal Cycles On The Retentive Force Of Locator And Locator R-Tx Attachments, Jidapha Tangtrongmit
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aims to assess the implant of varying angulations and insertion-removal cycles on the retentive force of 2 denture attachments. The blocks were produced via 3D printing and divided into 2 groups: the LOCATOR attachment with orange retentive inserts and the LOCATOR R-Tx attachment with pink retentive inserts. Each group was divided into 3 subgroups based on implant angulation at 0-, 15-, and 30-degree implant angulation. The insertion-removal cycles were performed in 37 ±1°C deionized water using an INSTRON E1000 machine. Retentive force tests were conducted using a universal testing machine at baseline, after 270, 540, 810, 1080, 2160, …
The Effect Of Water Sorption And Solubility On Surface Properties Of Cad/Cam Materials, Chatchalanya Wansudon
The Effect Of Water Sorption And Solubility On Surface Properties Of Cad/Cam Materials, Chatchalanya Wansudon
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objective of this research was to examine sorption, solubility, and surface properties of three widely used CAD/CAM materials: resin composite (Cerasmart; CS), polymer-infiltrated ceramic network (Vita Enamic; VE) and lithium disilicate glass ceramic (IPS e.max CAD; LS). 45 rectangular-shaped specimens were prepared and immersed in deionized water for varying durations. These specimens were then subjected to evaluate their water sorption, solubility, surface roughness, and surface hardness. The results showed that immersion time significantly impacted water sorption of the tested materials (P.05). The 2-way ANCOVA revealed that water immersions had significant effects on surface hardness among tested CAD/CAM blocks (P.05). …
The Effect Of Universal Adhesives On Repairability Of Four Cad/Cam Blocks, Tanyaporn Treyasorasai
The Effect Of Universal Adhesives On Repairability Of Four Cad/Cam Blocks, Tanyaporn Treyasorasai
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to assess the repairability among four CAD/CAM blocks repaired with different universal adhesives after aging. The CAD/CAM blocks evaluated were polymer infiltrated ceramic network (VE), zirconium-reinforced lithium silicate ceramic (VS), feldspathic ceramic (VM), and lithium disilicate ceramic (IE). Each original block was prepared to create 120 specimens. After 10,000 cycles of thermocycling, surface pretreatment of each specimen was carried out by grinding and etching with a 9% hydrofluoric acid. Subsequently, specimens were divided into three groups: Clearfil™ TRI-S BOND (CUB), Scotchbond Universal Plus® (SUP), and Monobond N® (MN). Resin composite was then applied. All samples underwent another …
Microleakage Of Class Ii Restoration Using Short-Fiber Reinforced Flowable Resin Composite, Parichat Panyawisitkul
Microleakage Of Class Ii Restoration Using Short-Fiber Reinforced Flowable Resin Composite, Parichat Panyawisitkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective. With short-fiber reinforced resin composite (SFRC), improved mechanical strength and good workability could be achieved within a single material. However, there remains concerns related to microleakage, which could restrict the application of this material in certain clinical situations. Materials and Methods. Class II cavities were prepared in 80 premolars. Each group, consisting of 10 specimens, was restored with different materials: flowable bulk-fill SFRC (EverX Flow, EXF), bulk-fill SFRC (EverX Posterior, EXP), flowable bulk-fill (Tetric N-Flow, TNF), and conventional resin composite (Z350 XT, Z350). The specimens were divided into two subgroups: one underwent thermocycling of 20,000 cycles, while the other …
Gene Expression Of Soft Tissue Covering Bone Grafted With Xenograft In Human, Atithan Termworasin
Gene Expression Of Soft Tissue Covering Bone Grafted With Xenograft In Human, Atithan Termworasin
Chulalongkorn University Theses and Dissertations (Chula ETD)
Soft tissue is one of many factors that influence the esthetic success of dental implants. This study explores soft tissue reaction between soft tissue covering bone grafted with xenograft and soft tissue in normal healing by analyzing the levels of inflammatory cytokine including TNF-α, IL-6, IL-8 and NF-kB1. Soft tissues are collected after 4 to 6 months of healing and analyzed by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The result shows that the relative gene expression of TNF-α, IL-6, IL-8, and NF-kB1 are not significantly different in soft tissue covering xenograft compared to the ones in soft tissue covering …