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The Effectiveness Of Asiaticoside On Osteogenic Differentiation Ability In Aging Human Periodontal Ligament Cells In Vitro, Adisa Suthirathikul
The Effectiveness Of Asiaticoside On Osteogenic Differentiation Ability In Aging Human Periodontal Ligament Cells In Vitro, Adisa Suthirathikul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Retaining teeth is essential for the quality of life of the aging population. However, aging has biological effects in periodontal ligament cells with significant changes in their proliferative rate and regenerative function. Asiaticoside, extracted from Centella asiatica, was found to have potential in osteogenic differentiation and mineralization in human periodontal ligament cells (HPDLCs). The aim of this study is to investigate the effect of asiaticoside on the aging HPDLCs osteogenic differentiation and mineralization. Aging HPDLCs, retrieved from patients age 60 and above, were confirmed aging status using Senescence-Associated ß -Galactosidase assay. The cells were incubated with various concentrations of asiaticoside …
Investigation Of Copper And Zinc Contaminations In Bioponic Systems Using Chicken Manure Fertilizer, Satja Aksorn
Investigation Of Copper And Zinc Contaminations In Bioponic Systems Using Chicken Manure Fertilizer, Satja Aksorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The bioponic system is a system that utilizes biological waste as a nutrient-rich source such as chicken manure fertilizer for plant growth integrated with aquaponic. At present, chicken manure fertilizer is contaminated with Cu and Zn may have the potential health effects through vegetable consumption. The objective of this study was to evaluate nitrogen transformation, nitrogen recovery, and plant growth in chicken manure-based bioponics at Cu (50–150 mg/kg) and Zn (200–600 mg/kg) supplementation and investigate the effects of Cu and Zn supplementations on plant bioaccumulation, root microbial community, and dietary health risk. Cu and Zn concentrations were analyzed using inductively …
Ecological Risk Assessment Of Microplastics And Heavy Metals In Central Industrial Wastewater Treatment Plant, Thamonwan Phuprasert
Ecological Risk Assessment Of Microplastics And Heavy Metals In Central Industrial Wastewater Treatment Plant, Thamonwan Phuprasert
Chulalongkorn University Theses and Dissertations (Chula ETD)
Microplastics become a big concern in environment. Their small particle size can absorb a variety of pollutants from the nearby environment when discharged into the environment, especially heavy metals. Central industrial wastewater treatment plant (WWTP) is an important source of microplastics in the environment. In this study, wastewater and sludge were collected from two industrial estates in Thailand. Wastewater was collected from 4 points i.e., influent, post grit chamber, post aeration tank, and post sedimentation tank while sludge was collected from sludge storage. Concentration of microplastics, heavy metals and heavy metals on microplastics were examined to evaluate ecological risk assessment. …
Queering The Migrant Experience: Gay Filipino Workers’ Performance Of Gender And Sexual Identities In Bangkok, Hans Kevin Madanguit
Queering The Migrant Experience: Gay Filipino Workers’ Performance Of Gender And Sexual Identities In Bangkok, Hans Kevin Madanguit
Chulalongkorn University Theses and Dissertations (Chula ETD)
The Philippine migration scholarship heavily focuses on heteronormative issues that depict the migrant workers as heterosexuals. Due to this trend, there is a scarcity of studies on overseas Filipino workers who identify as part of the Lesbian, Gay, Bisexual, Transgender, and Queer (LGBTQ+) Community. Hence, this qualitative research focuses on eleven documented Filipino workers in Thailand who are males assigned at birth and identify as gay homosexuals. Individual informal interviews were employed to investigate how they practice their non-normative gender and sexuality, given their multiple yet intersectional social identities in a country with vibrant queer cultures. It also probes the …
A Study Of Covid-19 Crisis Communication In Myanmar (January 2020-January 2021) : Divergences Between Ministry Of Health And Sports And State Counsellor, Aye Pa Pa Moe
Chulalongkorn University Theses and Dissertations (Chula ETD)
Effective crisis communication is critical in a country like Myanmar, which has a vulnerable population and a newly democratically elected government. During the COVID-19 pandemic, Myanmar's government established multi communication channels to interact directly with its citizens and other actors, including government agencies, allowing for active stakeholder participation in crisis management. The purpose of this research is to identify the Myanmar government's crisis communication strategy in managing the COVID issue at the national level from January 2020 to January 2021. The study will take a three-stage approach to crisis management, with pre-crisis, crisis, and post-crisis stages. It also explored Myanmar's …
Ecological System And Psychosocial Well-Being Of Myanmar Migrant Workers During The Covid-19 Pandemic "A Case Of Mahachai", Susan Moe Kyaw
Ecological System And Psychosocial Well-Being Of Myanmar Migrant Workers During The Covid-19 Pandemic "A Case Of Mahachai", Susan Moe Kyaw
Chulalongkorn University Theses and Dissertations (Chula ETD)
The complex interactions in each ecological setting influence the psychosocial well-being of a person. Migrant workers’ well-being is highly influenced by their working conditions, security, and social relationship. They encounter not only work-related stress, and discrimination, but are also caused by socio-economic changes during the global crisis such as the Covid-19 outbreak. Prior to the COVID-19 outbreak in Thailand, Mahachai has been a place of many studies and research as it hosts thousands of migrant workers, and its major seafood production, fishery, metal, and other factories in its area. It draws the attention of migration scholars, policymakers, and researchers to …
Does South Korea's Melon Chart Ranking Influence K-Pop Artists' Youtube Views?, Farsai Tipsotikul
Does South Korea's Melon Chart Ranking Influence K-Pop Artists' Youtube Views?, Farsai Tipsotikul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Although K-pop has been diffused throughout the world by YouTube and become globalized, artist’s success is still evaluated by their reputation within the domestic market. Amidst the intense competition in the South Korean music industry, it’s inevitable that artists would hope their songs perform well on the domestic chart as it’s a way to gain public attention. Thus, it leads to the question; “Are some artists putting too much stress on the domestic performances when the global market is also an opportunity?”. To answer the question, this study analyzes the data by quantitative approach to examine whether Melon chart ranking, …
Factors Influencing On Successfulness Of Thailand's Banning Policy On Single-Use Plastic Cups And Straws, Natcha Stithyudhakarn
Factors Influencing On Successfulness Of Thailand's Banning Policy On Single-Use Plastic Cups And Straws, Natcha Stithyudhakarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The present research aims to find out factors influencing on successful implementation of Thailand's SUP cups and straws banning policy which has been implementing since January 2022. Target groups of the study are consumers in Bangkok and vicinity (Nonthaburi, Pathum Thani, Samut Prakan, Samut Sakhon and Nakhon Pathom). As serious Covid-19 pandemic situation during the data collection period (April 18-June16, 2022), the questionnaire survey was conducted via Google form online survey platform, but additional onsite survey at a school in Bangkok was conducted on June 16, 2022, where the pandemic situation has become better. Total 718 respondents in the study …
Factors Affecting The Achievement Of Eps Test Of Proficiency In Korean Of Thai Workers : Focusing On Korean Language Schools In Udon Thani, Puttarak Kamsing
Factors Affecting The Achievement Of Eps Test Of Proficiency In Korean Of Thai Workers : Focusing On Korean Language Schools In Udon Thani, Puttarak Kamsing
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research investigated the important factors affecting the achievement of EPS Test of Proficiency in Korean of Thai workers. The objectives of this research were 1) to explore Thai workers’ problems in Korean language learning, and 2) to investigate the factors affecting the achievement of EPS Test of Proficiency in Korean of Thai workers. The samples consisted of 214 Thai workers studying in 3 Korean language schools in Udon Thani, Thai workers who are working in South Korea, and 4 teachers who taught the Korean language to Thai workers in 3 Korean language schools in Udon Thani. This research employed …
Contributing Factors In Aircraft Accidents Triggering Fatalities, Prakayphet Chalayonnawin
Contributing Factors In Aircraft Accidents Triggering Fatalities, Prakayphet Chalayonnawin
Chulalongkorn University Theses and Dissertations (Chula ETD)
Air transport has always been considered the safest way to travel. It has contributed numerous economic and social benefits, for instance, connecting multimodal modes of transportation, increasing employment, driving global and local economic activities, reducing travel time and generating trade, tourism, and services, connecting people and countries, and supporting humanitarian activities in a remote rural area. Historical records on the fatality ratio suggested that air transportation has the highest likelihood of accidents (occurrences with fatalities) when compared to all other transportation modes, including highway, railroad, and water. Then, when an accident occurs, it largely affects various stakeholders. This study aims …
The Healthcare Information For Patient Referral, Veerawan Aumpanseang
The Healthcare Information For Patient Referral, Veerawan Aumpanseang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Despite the cooperative sharing of Health Information Exchange (HIE), various distinct limitations and barriers are found, i.e., substantial time and resources are being used to achieve health information. This paper aims to investigate the limits of healthcare information sharing policy implementation and assorts the critical and shareable set of relevant healthcare information to patient referral systems in Thailand. Mixed-methods research methodology, both quantitative and qualitative mechanisms are conducted. The study results present the correlation between the current healthcare information exchange among the hospitals in patient referral systems and the limitations of implementing the HIE policy composed of technical, economic, political, …
Development Of Novel Point-Of-Care-Testing For Oxalate Detection In Urine Based On Oxalate-Binding Ligands, Naruerat Joybumrung
Development Of Novel Point-Of-Care-Testing For Oxalate Detection In Urine Based On Oxalate-Binding Ligands, Naruerat Joybumrung
Chulalongkorn University Theses and Dissertations (Chula ETD)
Kidney stone disease is the third most common urinary tract disease worldwide that is more prevalent in the tropics. In Thailand, the highest stone prevalence up to 17% is reported in the Northeast. The disease is highly recurrent with a recurrence rate of about 50% within 5-10 years. The most common type of kidney stones is calcium oxalate (CaOx), and an increased urinary oxalate excretion is an important risk factor. Most of kidney stone patients have a hyperoxaluria condition (urinary oxalate >40 mg/day or >0.46 mmol/day). Therefore, urinary oxalate measurement has been widely used for estimating the risk of CaOx …
Epigenetic Regulation Of Reactive Oxygen Species-Induced Tumor Progression In Hepatocellular Carcinoma, Suchittra Phoyen
Epigenetic Regulation Of Reactive Oxygen Species-Induced Tumor Progression In Hepatocellular Carcinoma, Suchittra Phoyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Oxidative stress is a consequence of an imbalance of antioxidants and reactive species. The most common form of reactive species is derived from oxygen, called reactive oxygen species (ROS). ROS involve in pathogenesis of several diseases including cancers. Cancer genesis and progression are contributed through both genetic and epigenetic mechanisms. Histone modification, a post-translational modification at histone tails, is one of the epigenetic mechanisms known to participate in carcinogenesis and tumor progression. Although the ROS-induced histone modification alteration has been demonstrated in some cancers, the change in histone methylation and gene expression by ROS in hepatocellular carcinoma (HCC) is scarcely …
Development Of A Human Viable Heavy Chain Antibody Against The Alpha Folate Receptor As A Molecule For Targeted Application By Phage Display Technology, Nattihda Parakasikron
Development Of A Human Viable Heavy Chain Antibody Against The Alpha Folate Receptor As A Molecule For Targeted Application By Phage Display Technology, Nattihda Parakasikron
Chulalongkorn University Theses and Dissertations (Chula ETD)
Alpha folate receptor (FRα) is currently under investigation as a target for the treatment of patients with non-small-cell lung cancer (NSCLC), since it is highly expressed in tumor cells but is largely absent in normal tissue. In this study, a novel human variable domain of a heavy-chain (VH) antibody fragment specific to FRα was enriched and selected by phage bio-planning. The positive phage clone (3A102 VH) is specifically bound to FRα and also cross-reacted with FRβ, as tested by ELISA. Clone 3A102 VH was then successfully expressed as a soluble protein in an E. coli shuffle strain. The obtained soluble …
Designing An Extracorporeal Plasma Filtration Column With Specific Binding To Dengue Virions, Sasiwimon Thonghong
Designing An Extracorporeal Plasma Filtration Column With Specific Binding To Dengue Virions, Sasiwimon Thonghong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Dengue infection is one of the major public health concerns and no specific treatment is currently available. An extracorporeal system is one of the alternative treatment in severe dengue patients with impending shock. However, the commercial immunoadsorption devices were size-exclusive, non-selective designed for multipurpose thus not specific for dengue syndrome. This study focuses on establishing the flavivirus-specific (4G2) antibody entrapped into the alginate bead. Results showed that the viral titer reduced more than 90 % after 3 h running on a simulated circuit quantified by reverse transcription qPCR and plaque titration assay. The experiments were repeated with two biological replicates …
Impact Of Bacterial Dna In Severity Of Sepsis, Warerat Kaewduangduen
Impact Of Bacterial Dna In Severity Of Sepsis, Warerat Kaewduangduen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Bacterial sepsis can be caused by intestinal damage, leading to the passage of viable bacteria and their components into the circulatory system. Bacterial-free DNA in the blood during sepsis can be derived from bacterial death and intestinal translocation of free DNA in the gut contents. The presence of bacterial DNA in combination with lipopolysaccharide (LPS) can lead to more serious outcomes of sepsis. To explore the impact of bacterial-free DNA in sepsis, animal and macrophage experiments were performed. Firstly, the appearance of bacterial-free DNA and bacteriome in the blood was presented in mice with cecal ligation and puncture sepsis. Secondly, …
Dosimetric Impact Of Interplay Effects Instereotactic Body Radiation Therapy (Sbrt) Lung Cancer, Vanida Poolnapol
Dosimetric Impact Of Interplay Effects Instereotactic Body Radiation Therapy (Sbrt) Lung Cancer, Vanida Poolnapol
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lung cancer patient breathing induced interplay effects in the volumetric modulated arc therapy (VMAT) together with stereotactic body radiation therapy (SBRT), which lead to non-uniform doses within the target volume and unwanted dose to the surrounding tissues. The purpose of this study was to investigate the dosimetric impact of interplay effects in 6 MV FFF of photon beams for lung cancer and to calculate the novel interplay effects factor (IEF) for VMAT-SBRT lung cancer. This study performed the static and dynamic measurements using two dimensions (2D) robotic platform and diode array for 3D-conformal radiation therapy (3D-CRT) plans, while using three …
Alteration Of Extracellular Matrix Components In Anterior Pituitary Gland Of Neonatal Rats Induced By Maternal Bisphenol A (Bpa) Diet During Pregnancy, Bumpenporn Sanannam
Alteration Of Extracellular Matrix Components In Anterior Pituitary Gland Of Neonatal Rats Induced By Maternal Bisphenol A (Bpa) Diet During Pregnancy, Bumpenporn Sanannam
Chulalongkorn University Theses and Dissertations (Chula ETD)
Bisphenol A (BPA) is a well-known endocrine-disrupting chemical that is widely used in the resin and plastic manufacturing sectors. Prenatal exposure to this chemical results in changes of pituitary hormone-producing cells. However, there have been no reports of the alterations of extracellular matrix (ECM) which play a role in controlling cell activities in the pituitary gland. In the present study, a daily oral dose of 5,000 µg/kg BW of BPA was administered to pregnant rats. ECM-producing cells, i.e., pericytes and folliculostelate cells, ECM products, and ECM regulators were investigated in the neonatal anterior pituitary at day 1 at the histology …
Modified Pre-Pcr Protocol For Detection Of Bacteria In Blood Samples Of Patients With Sepsis, Phanni Wanthong
Modified Pre-Pcr Protocol For Detection Of Bacteria In Blood Samples Of Patients With Sepsis, Phanni Wanthong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Sepsis and septic shock are leading causes of deaths in hospitals. The most common cause of blood stream infection is bacteria. To reduce mortality, a rapid and accurate bacterial detection in sepsis patients is essential. It is necessary to identify pathogens based on their species for appropriate choice of antibiotics. In this study, we aimed to improve the pre-PCR procedure for detection of bacteria in blood samples and to compare the performance between real-time PCR, conventional PCR and blood culture. Blood samples from 16 sepsis patients were separated into whole blood, plasma, buffy coat and white blood cell. Bacterial nucleic …
Effects Of Strongyloides Stercoralis Antigens On Immune Responses In Dendritic Cells, Vittawin Sutaveesup
Effects Of Strongyloides Stercoralis Antigens On Immune Responses In Dendritic Cells, Vittawin Sutaveesup
Chulalongkorn University Theses and Dissertations (Chula ETD)
Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis. Infection in the immunocompromised hosts such as organ transplants, SLE patients, or HIV and HIPV infected patients can cause severe strongyloidiasis. Infected patients have high levels of Th2, and Treg related cytokines, including IL-4, IL-5, IL-9, IL-10, IL-13, and TGF-β. These cytokines are then decreased to normal levels after the treatments. Parasites have several mechanisms to suppress or evade the host’s immune response, such as suppressing the expression of co-stimulatory molecules on antigen-presenting cells (APCs), stimulating apoptosis of APCs, or stimulating Th2 and Treg responses. Dendritic cells (DCs) play a crucial …
Attenuation Of Chronic Kidney Disease (Ckd) And Uremia Induced Gut-Leakage By Lactobacillus Rhamnosus L34, A Probiotic Derived From Thai Population, In 5/6 Nephrectomy Model Mice; An Experimental Study, Somkanya Tungsanga
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Although pathogenic gut microbiota causes gut leakage, increases translocation of uremic toxins into circulation, and accelerates CKD progression, the local strain of Lactobacillus rhamnosus L34 (L34) might attenuate gut leakage. We explored the effects of L34 on kidney fibrosis and levels of gut-derived uremic toxins (GDUTs) in 5/6-nephrectomy (5/6 Nx) mice. Methods: At 6 weeks post-5/6 Nx in mice, either L34 (1x106 CFU) or phosphate buffer solution (as 5/6 Nx control) were daily fed for 14 weeks. In vitro, the effects of L34-conditioned media with or without indoxyl sulfate (a representative GDUT) on inflammation and cell integrity (transepithelial electrical …
Development Of A Personalized Workplace Learning Program To Enhance The Cultural Intelligence Of Cabin Crew From International Airlines, Dech-Siri Nopas
Development Of A Personalized Workplace Learning Program To Enhance The Cultural Intelligence Of Cabin Crew From International Airlines, Dech-Siri Nopas
Chulalongkorn University Theses and Dissertations (Chula ETD)
This thesis study employed R&D as a research methodology, and employ a qualitative method to collect the data. It aimed to 1) explore the background of cabin crew from the international airlines concerning cultural intelligence from seven key informants; 2) develop personalized workplace learning programs to enhance the cultural intelligence of cabin crew from the international airlines with seven key informants of the cabin crew and the researcher as a facilitator, and 3) propose a guideline for developing a personalized workplace learning program to enhance the cultural intelligence of cabin crew from the international airlines by developing all the findings …
Predictors Of Time To Hospital Among People With Colorectal Cancer, Saimai Tumwijit
Predictors Of Time To Hospital Among People With Colorectal Cancer, Saimai Tumwijit
Chulalongkorn University Theses and Dissertations (Chula ETD)
This retrospective, cross-sectional study aims to describe time to hospital, relating factors, and determine predictors of time to hospital among people with colorectal cancer. Multistage random sampling was used to yield a probability of 240 people with colorectal cancer visiting medical and surgical outpatient departments, oncology units, radiology units, endoscopic units, and inpatient departments. Research instruments consisted of personal information sheet, time to colorectal cancer diagnosis questionnaire, knowledge about colorectal cancer questionnaire (KR-20 = .786), The modified illness perception questionnaire-revised (a = .674 - .836), health care seeking behavior questionnaire (a = .706), and perceived seriousness of warning signs and …
Effect Of Different Placement Methods On Voids Formationin Class Ii Cavity Restored With Bulk-Fill Resin Composite, Parinthorn Siyoputhawong
Effect Of Different Placement Methods On Voids Formationin Class Ii Cavity Restored With Bulk-Fill Resin Composite, Parinthorn Siyoputhawong
Chulalongkorn University Theses and Dissertations (Chula ETD)
The aim of this study was to evaluate void formation by micro-computed tomography in two-surface Class II cavities restored with two bulk-fill resin composites using different placement methods. Standardized Class II cavities were prepared in forty intact human maxillary first premolar teeth. The teeth were randomly divided into four groups and restored with (n=10): 1) one bulk placement with hand instrument; 2) two-bulk placement with hand instrument; 3) one bulk placement with injectable dispenser; 4) one bulk placement with injectable dispenser and sonic-activated handpiece. Percentage of void formation in the entire restoration was evaluated. Welch's ANOVA and Games-Howell post hoc …
Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi
Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi
Chulalongkorn University Theses and Dissertations (Chula ETD)
In the clinical situation, pulp exposure can happen during caries removal procedures. Inflammation can occur on the pulp of the exposed area because of infiltration of bacteria from caries or as a result of pulp reaction to caries removal drilling. When the pulp was exposed during the caries removal procedure, traditional methods to preserve the pulp such as pulp capping and pulpotomy were performed. The ideal treatment outcome of the pulp exposure is to regain the primary structure of tubular dentin as well as maintain the vitality and healthiness of the dental pulp Thus, good dental materials or drugs used …
Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak
Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak
Chulalongkorn University Theses and Dissertations (Chula ETD)
The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …
Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn
Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The complete regeneration of periodontal tissues following current periodontal therapy remain challenging and unpredictable. Nucleoside-modified messenger RNA (mRNA) technology can be a promising novel platform in regenerative medicine. The aims of this study were to evaluate whether pseudouridine modified mRNA encoding vascular endothelial growth factor (VEGF) could induce VEGF production in human periodontal ligament cells (PDLCs) and this translated protein function by promoting in vivo blood vessel formation using chorioallantoic membrane (CAM) assay. Isolated PDLCs from healthy periodontal tissue were transfected with modified mRNA encoding VEGF (VEGF mRNA) complexed with a transfecting agent, Lipofectamine 2000 (L2000) and L2000 alone (control). …
Sustainable Two-Step Minlp Heat Exchanger Network Synthesis With Practical Detailed Design, Thanawat Boonvaerojkul
Sustainable Two-Step Minlp Heat Exchanger Network Synthesis With Practical Detailed Design, Thanawat Boonvaerojkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Due to sustainable development that focuses on energy conservation, decreasing emissions and environmental effect, the research of heat exchanger network synthesis (HENS) is still necessary and challenging. Most academic studies in the subject of HENS aim on reducing total annual cost (TAC) through optimal topology design without the detailed design costs of each heat exchanger in the network, resulting in conceptual HEN designs. Hence, the detailed design of shell and tube heat exchangers is recalculated for TAC and corrected area costs, including the pumping costs from overall pressure drop of heat exchangers. For a shell and tube heat exchanger, its …
Cholesterol-Lowering And Probiotic Properties Of Selected Lactic Acid Bacteria, Engkarat Kingkaew
Cholesterol-Lowering And Probiotic Properties Of Selected Lactic Acid Bacteria, Engkarat Kingkaew
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ninety strains of lactic acid bacteria (LAB) isolated from fermented foods and bark of Tamarindus indica were screening cholesterol-lowering effects, selected and identified based on the phenotypic, chemotaxonomic characteristics and 16S rRNA gene sequence analysis. They were identified as Companilactobacillus farciminis (5 strains), C. formosensis (10 strains), C. futsaii (6 strains), C. pabuli (4 strains), Enterococcus thailandicus (2 strains), Lactiplantibacillus argentoratensis (3 strains), Lactiplantibacillus pentosus (10 strains), Lactiplantibacillus plantarum subsp. plantarum (17 strains), Lactococcus lactis subsp. lactis (2 strains), Lentilactobacillus buchneri (8 strains), Limosilactobacillus fermentum (2 strains), P. pentosaceus (12 strains) and each of En. durans, En. hirae, En. lactis, …
Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri
Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Fine particulate matter (PM2.5) can cause skin damage through the induction of oxidative stress in epidermis. Antioxidants help counteract with cellular oxidant species and maintain cell homeostasis. This study aims to examine the protective effect of Thunbergia laurifolia lindl. ethanolic extract on PM2.5-mediated oxidative stress in epidermal keratinocytes. High-performance liquid chromatography with diode array detection was conducted for quantitative analysis of rosmarinic acid content in the extract. Cell viability was detected by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. Intracellular ROS was monitored using 2’,7’-Dichlorodihydrofluorescein diacetate. Proteins related to p62-Keap1-Nrf2 signaling pathway were examined by western blot analysis. Expressions of Nrf2 and p62 …