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Detection Of Arsenite-Oxidizing Bacteria In Groundwater From A Gold Mine Under Different Geochemical Environments, Supeerapat Kraidech
Detection Of Arsenite-Oxidizing Bacteria In Groundwater From A Gold Mine Under Different Geochemical Environments, Supeerapat Kraidech
Chulalongkorn University Theses and Dissertations (Chula ETD)
Millions of people around the world potentially expose to arsenic (As) contaminated groundwater. Besides, the dominant form of As in groundwater is arsenite (As+3) and is more toxic than arsenate (As+5). This study integrated the microbial investigation, geochemical modeling, and multivariate statistical analysis to investigate the arsenite-oxidizing bacteria community from As-contaminated groundwater and its environmental influencing factors in order to understand and further develop the in-situ arsenic bioremediation technology. Microbial investigation was focusing on the detection and identification of native arsenite-oxidizing bacterial community using PCR-DGGE, cloning and sequencing of arsenite oxidase (aoxB) gene amplicons. Nine groundwater samples were collected from …
Characterization Of Carbon Storage Regulator A (Csra) Homolog As A Global Gene Regulator In Leptospira Spp., Theerapat Phoka
Characterization Of Carbon Storage Regulator A (Csra) Homolog As A Global Gene Regulator In Leptospira Spp., Theerapat Phoka
Chulalongkorn University Theses and Dissertations (Chula ETD)
Leptospira spp. consist of non-pathogenic and pathogenic species. The bacteria can survive in diverse environments and therefore need mechanisms of rapid adaptation to environmental changes. However, molecular mechanisms of stress response in Leptospira are not fully understood. Carbon Storage Regulator A (CsrA) is a well-characterized, conserved, post-transcriptional global regulator that plays a critical role in response to environmental changes in many bacteria. CsrA has been reported to regulate several pathways such as glycogen metabolism, motility, biofilm formation, and virulence-associated genes. Based on available genomics data of Leptospira spp., we found putative csrA homolog, approximately 50% similarity with csrA of E. …
Comparative Evaluation Of Mineral Trioxide Aggregate And Acemannam For Partial Pulpotomy In Permanent Teeth With Incomplete Root Formation, Thuy Tien Vu
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mineral trioxide aggregate (MTA), the standard pulp capping material, has disadvantages, including long setting time and high cost. Along this line, acemannan, a polysaccharide extracted from aloe vera, would be a promising biomaterial for vital pulp therapy as it is shown to induce mineralized bridge formation in animal and clinical studies. In this study, the impact of MTA and acemannan sponges on partial pulpotomized permanent teeth has been evaluated. For the research design, fifty immature permanent teeth with caries or accident-induced pulp exposure were collected and assessed. After partial pulpotomy, the teeth were randomly allocated into the treatment group using …
The Differential Dendritic Cell Properties In Response To Candida Albicans And Non-Albican Candida Β-Glucan, Truc Thi Huong Dinh
The Differential Dendritic Cell Properties In Response To Candida Albicans And Non-Albican Candida Β-Glucan, Truc Thi Huong Dinh
Chulalongkorn University Theses and Dissertations (Chula ETD)
Increased mortality and antifungal drug resistance of non-albicans Candidiasis patients have become an alarming in health problems nowadays, whereas profound knowledge of their pathogenesis mechanisms is still limited. The immune responses of innate and adaptive immunity play a crucial role in protecting from Candida infections. Dendritic cells (DCs) are the most notable because of their roles in bridging an innate immunity and specific adaptive immunity to eliminate fungal invasion. It is little known about DC functions in non-albicans Candida (NACs) infections. Besides, there is less study on evaluating the DC properties upon the interaction between DCs and NACs-derived ß-glucans. Therefore, …
Servitization Model For Chemical Industry In Thailand, Tanyaporn Kanignant
Servitization Model For Chemical Industry In Thailand, Tanyaporn Kanignant
Chulalongkorn University Theses and Dissertations (Chula ETD)
Chemical industry traditionally produces and sells tangible goods in large volume and high price cometition. Recently, firms in chemical industry provide additional services to their customers. Several manufacturers change from tangible product suppliers to both product and service providers. This movement is called servitization (Vandermerwe & Rada, 1988). Chemical servitization levels can be classified into 4 categories which are product only, service added to the product, service differential the product and service is the product (Thoben, Eschenbacher, & Jagdev, 2001). The objectives of this paper are to construct servitization framework for chemical suppliers to shift to product service integration and …
Sustainable Business Development Through Sufficiency Economy Practice : A Case Study Of Thai Listed Companies, Veerawin Korphaibool
Sustainable Business Development Through Sufficiency Economy Practice : A Case Study Of Thai Listed Companies, Veerawin Korphaibool
Chulalongkorn University Theses and Dissertations (Chula ETD)
The purpose of the research is to determine the sufficiency economy philosophy (SEP) performance through annual and sustainable development reports and simplify the outcomes by representing through a scoring system. Each of the SEP elements was aligned with each GRI sub-topic to generate a SEP scoring system. Then, the results from the scoring system were verified by testing the relationship between SEP performance and firm-specific risk of 34 Thai listed companies from 2013 to 2018 via panel data analysis. The global reporting initiative (GRI) standards were used as the fundamental scoring system as it's widely used and accepted in academic …
Parasite: A Reflection On Social And Economic Disparities In South Korea, Wilasinee Nichasuk
Parasite: A Reflection On Social And Economic Disparities In South Korea, Wilasinee Nichasuk
Chulalongkorn University Theses and Dissertations (Chula ETD)
The purpose of this research is to analyze a reflection of social problems in "Parasite" film. The study is a qualitative research that uses semiology theory to analyze the social problems in the film through elements such as visual sign, scene, dialogue etc. The results of these analyses show that "Parasite" film reflects the social problems in South Korea through a visual sign, scene, dialogue such as disparity social class problems. These problems that occur in South Korea are partly due to a result of the rapid change in South Korea society and adoption of the capitalist system to develop …
Supply Chain Collaboration For Sustainability In Thailand's Dairy Industry, Virayos Vajirabhoga
Supply Chain Collaboration For Sustainability In Thailand's Dairy Industry, Virayos Vajirabhoga
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study gathered potential 95 variables from a literature review. Through the process of expert review, these were refined into 49 critical variables. Moreover, pilot study, with participation from co-operatives and farmers, aims to develop conceptual frameworks in six areas: performance and commitment, internal and external collaboration, measurement and evaluation, joint operation, sharing and innovation, and negotiation. Furthermore, 26 factors were identified clearly by exploratory factory analysis. The main study was conducted via a paper-based questionnaire with 1,224 respondents nationwide. The proposed 11 hypotheses attempted to identify the structural relationships among the constructs in the model. The result confirmed the …
Neutrophil Extracellular Traps (Nets) Formation Of Fcgr2b Deficient Mice In Lupus Mouse Model With Ischemic Reperfusion Injury, Wilasinee Saisorn
Neutrophil Extracellular Traps (Nets) Formation Of Fcgr2b Deficient Mice In Lupus Mouse Model With Ischemic Reperfusion Injury, Wilasinee Saisorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Acute kidney injury (AKI) caused by renal ischemic reperfusion (I/R) is the most prevalent cause of morbidity and mortality in patients. Neutrophil extracellular traps (NETs) are important for the progression of lupus nephritis after renal I/R injury. Because neutrophils are the first immune cell that respond to tissue damage, neutrophils might be an important cell that determines renal I/R injury. Additionally, NET components, including cell-free DNA, histone, nucleosome, and cytoplasmic protein compartment release the auto-antigens that accelerate lupus disease activity. Therefore, this project aimed to compare NETs formation in several internal organs following renal I/R injury in Fcgr2b deficient (Fcgr2b-/-) …
Assessments Of Hydraulic Responses To Climate Variability Of Dominant Trees In Successional Forests In Khao Yai National Park For Environmental Management, Weerapong Unawong
Assessments Of Hydraulic Responses To Climate Variability Of Dominant Trees In Successional Forests In Khao Yai National Park For Environmental Management, Weerapong Unawong
Chulalongkorn University Theses and Dissertations (Chula ETD)
In Southeast Asia, tropical forests have been increasingly threatened by human disturbances and climate change-induced water stress. However, uncertainty remains as to how these forests would respond to environmental changes under water deficit conditions. Here, we conducted a study of seasonal variation in leaf water status and hydraulic properties of dominant tree species across forest successional stages in Khao Yai National Park, Thailand. Specifically, we measured midday leaf water potential (Ψmd) during the wet and dry season, and assessed branch xylem vulnerability at which 50% is lost (P50). The associations between P50 and maximum xylem hydraulic conductivity (Ksmax) and maximum …
Innovative Zero-Waste System For Building Wastewater Recycling And Food Waste Management, Thammananya Sakcharoen
Innovative Zero-Waste System For Building Wastewater Recycling And Food Waste Management, Thammananya Sakcharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Urbanization leads to concerns about environmental impacts due to the increasing food waste and wastewater generation. The study aims to develop an innovative zero-waste system for food waste and wastewater management in the building using the combination of a single-stage anaerobic digester and the Moving Bed Biofilm Reaction-Membrane Bioreactor (MBBR-MBR). The comprehensive analysis for innovative zero-waste system development is conducted including (1) survey and interview the target market group (universities) to determine the factors affecting the intention to use the zero-waste system ; (2) develop a zero-waste system prototype, and test operation for measuring the performances ; (3) evaluate the …
Over-Expression Of Mir-223 Induces M2 Macrophage Through Glycolysis Alteration And Attenuates Lipopolysaccharide-Induced Sepsis Mouse Model, A Proposal Sepsis Cell-Based Therapy, Dang Phi Cong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Sepsis, a systemic infection with excessive inflammatory symptoms due to dysregulation of immune responses, remains the most concerned health-care problem worldwide with the mortality rate critically beyond the number of death cases in HIV, cancers or stroke. While it is well documented that pathogenesis of sepsis is intimately involved in dysregulation of macrophages, great effort has been made to discover efficacious therapy to control macrophage responses. However, macrophages are the most versatile immune cells with large spectrum of heterogeneous phenotypes and functions. Recently, collection of insights unravel the crucial role of metabolism as an orchestrator of immune regulation, intervention on …
High-Mobility Group Box 1 (Hmgb1) Mediates Dna Methylation Preventing Genomic Instability, Papitchaya Watcharanurak
High-Mobility Group Box 1 (Hmgb1) Mediates Dna Methylation Preventing Genomic Instability, Papitchaya Watcharanurak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Global DNA hypomethylation promotes genomic instability through the accumulation of DNA damage and resulted in an increasing rate of mutation. To stabilize the genome, cells possess an epigenetic mechanism to reduce DNA tension that causes DNA damages, named Replication-Independent Endogenous DNA Double-Strand Breaks (RIND-EDSBs). Due to their biological roles, this type of physiologic EDSBs act as youth-associated genomic-stabilizing DNA gaps (Youth-DNA-GAPs). They are localized within methylated genome and maintained by non-histone HMGB1 and SIRT1 proteins. Reduction of RIND-EDSBs was found in hypomethylated genome, including cancer and aging. Therefore, this study aimed to explore the role of HMGB1-mediated DNA methylation in …
Development Of An Integrated Microfluidic System For Cell Sorting And Trapping—Considering Sorting Efficacy And Cell Viability, Thammawit Suwannaphan
Development Of An Integrated Microfluidic System For Cell Sorting And Trapping—Considering Sorting Efficacy And Cell Viability, Thammawit Suwannaphan
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study is divided into two phases. The first phase is about the investigation of cell viability and damage of white blood cells after sorting in both a spiral microchannel and contraction and expansion array (CEA) at appropriate flow rates for cell separation. Three techniques such as Trypan Blue staining, Scanning Electron Microscopy (SEM) and Wright-Giemsa staining are employed in the experiments. After flowing the sample through a whole setup of spiral microchannel at 2 ml/min, 85% of cells are viable while 30% of cells are significantly deformed and 2% of cells are seriously damaged in their intracellular structures. In …
Effect Of Food Impaction In The Interproximal Of Implant Supported Fixed Restoration Towards Oral Health Related Quality Of Life, Souknilan Chanthasan
Effect Of Food Impaction In The Interproximal Of Implant Supported Fixed Restoration Towards Oral Health Related Quality Of Life, Souknilan Chanthasan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objectives: To investigate the effect of food impaction between dental implant and adjacent teeth to the patients’ quality of life and periodontal/peri-implant tissue conditions. Materials and methods: Patients with implant supported single crown (ISSC) having implant checkup at the Faculty of Dentistry, Chulalongkorn University between July 2019 and July 2020 were recruited. Food impaction was evaluated by either patients’ experience or clinical examination by dentist. Self-administrative Oral impacts on Daily Performance questionnaire was used to assess the effect of food impaction to patients’ quality of life. Clinical and radiographic examination was performed to evaluate periodontal/peri-implant tissue conditions. Results: Totally 178 …
Metabolite Profiling And Gene Expression Analysis In Starch And Sugar Metabolisms Of Ripening Durian Durio Zibethinus Pulp, Lalida Sangpong
Metabolite Profiling And Gene Expression Analysis In Starch And Sugar Metabolisms Of Ripening Durian Durio Zibethinus Pulp, Lalida Sangpong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Durian (Durio zibethinus L.), an economically important fruit of Southeast Asian countries, expresses unique flavor at the edible ripe stage, resulting from metabolic changes during the ripening process. Nevertheless, the study in such modulations is still limited. In the first manuscript, we profiled metabolites from unripe and ripe pulps of durian cv. Monthong using capillary electrophoresis- (CE) and gas chromatography- (GC) time-of-flight mass spectrometry (TOF/MS) and obtained the total 94 ripening-related metabolites from a combination of the two datasets, of which we focused on flavor-related metabolites. For sugar, sucrose is the most abundant sugar that increased during ripening, and the …
Comparison Of The Accuracy To Predict Falls Of The Figure Of 8 Walk Test With And Without Dual-Task In Older Adults, Tidarat Nualyong
Comparison Of The Accuracy To Predict Falls Of The Figure Of 8 Walk Test With And Without Dual-Task In Older Adults, Tidarat Nualyong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: The figure of eight walk test (F8W) is a measurement tool that examines the ability to walk on a curved path. Adding secondary tasks to the F8W test may increase its ability to predict falls in older adults. This research aimed 1.) to examine the accuracy of the F8W test and the F8W test with dual-tasking for predicting future falls in older adults; 2.) to investigate the reliability and the concurrent validity of the F8W test with and without dual-task in older adults. Methods: Eighty older adults between the ages of 60 and 80 were recruited. At the beginning …
Classification Of Organoid Transcriptomic Profiles Unravelling Colorectal Cancer Molecular Subtypes, Pattarin Nuwongsri
Classification Of Organoid Transcriptomic Profiles Unravelling Colorectal Cancer Molecular Subtypes, Pattarin Nuwongsri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Colorectal cancer (CRC) is genetically and transcriptomically heterogeneous disease. Molecular subtyping of colorectal cancer using consensus molecular subtype (CMS) system demonstrated the potential predictive value for tumor progression and treatment response. However, the CMS system was developed from data of whole tissues containing both cancer and non-tumor transcripts components for classification which does not directly represent intrinsic heterogeneity of cancer cells. In this study genetic profiles of CRC organoids were investigated first, and the results indicate chromosomal instability (CIN) and microsatellite instability (MSI) as pathogenic pathways of CRC. Furthermore, the results also revealed diverse patterns of somatic mutations of these …
Clinical Parameters And Biological Markers Associated With Acute Severe Ocular Complications In Stevens-Johnson Syndrome And Toxic Epidermal Necrolysis In Thailand, Rawiphan Panpruk
Chulalongkorn University Theses and Dissertations (Chula ETD)
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe cutaneous adverse drug reactions with high mortality rates. Sequelae such as blindness remained even after recovery. Patients with SJS/TEN should be accurately diagnosed and received appropriate treatment as soon as possible. Therefore, factors for severity prediction are necessary. This study aimed to clarify clinical parameters and biological markers that can predict acute severe ocular complications (SOCs) in SJS/TEN. This retrospective cross-sectional study enrolled forty-seven SJS/TEN patients and divided them into two groups according to ocular severity at the acute onset, non-severe ocular complications group (N = 27) and severe ocular …
Virus Like Particle Displaying The Major Allergen Der P 1 As A Vaccine Candidate Against House Dust Mite Allergy, Sirikarn Jitthamstaporn
Virus Like Particle Displaying The Major Allergen Der P 1 As A Vaccine Candidate Against House Dust Mite Allergy, Sirikarn Jitthamstaporn
Chulalongkorn University Theses and Dissertations (Chula ETD)
House dust mite (HDM) represents one of the most important allergenic sources worldwide. HDM allergens trigger in atopic patients potent Th2-biased inflammatory responses leading to the development of allergic rhinitis/asthma and atopic dermatitis. The HDM allergic response is mediated mainly by the allergen-specific Th2 cells and IgE. Der p 1, a papain-like cysteine protease, is a major HDM allergen from the Dermatophagoides pteronyssinus species, inducing allergic sensitizations in around 80 percent of HDM allergic populations. Allergen-specific immunotherapy (AIT) represents a unique specific treatment capable to revert the house dust mite (HDM) allergic response into immune tolerance through repetitive subcutaneous or …
G6pd Activity, Oxidative Stress, And Alu Methylation In Hbv-Related Hepatocellular Carcinoma, Watcharapong Jugnam-Ang
G6pd Activity, Oxidative Stress, And Alu Methylation In Hbv-Related Hepatocellular Carcinoma, Watcharapong Jugnam-Ang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Hepatocellular carcinoma (HCC) is the third leading caused cancer death in worldwide. The effective diagnosis markers are needed because of the late diagnosis and low survival time. G6PD is the rate-limiting enzyme in pentose phosphate pathway (PPP) that elevated in several cancers including HCC. Alu element is a common repetitive sequence in human genome, that its methylation alteration has been reported in cancers. In this study, we aimed to investigate the level of blood G6PD activity, the expression of G6PD in HCC liver tissues, the level of Alu methylation in PBMCs and clinicopathological parameters of HCC patients. Blood G6PD activity …
Characterization Of Outer Membrane Vesicles From Escherichia Coli Bzb1107 And Bl21-De3 Strains, Sunisa Hankan
Characterization Of Outer Membrane Vesicles From Escherichia Coli Bzb1107 And Bl21-De3 Strains, Sunisa Hankan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vaccine is a prerequisite enhancer of acquired immunity used to protect against pathogen. The outer membrane vesicles (OMVs) are currently used for developing vaccine delivery system. Nevertheless, OMVs also contains outer membrane proteins (OMPs), which may be able to alter immunogenic effects and stability. The main objective of this study was to characterize OMVs for vaccine delivery system. Here, we compared OMV produced from BL21-DE3 and BZB1107 strains of Escherichia coli with or without expression of outer membrane proteins (OMPs), respectively. ClyA-GPF-6xhistidine tag gene was inserted into the vector. GFP was used as a representative antigen and an outer membrane …
Effect Of Erianthridin On Non-Small Cell Lung Cancer Cell Metastasis, Sutthaorn Pothongsrisit
Effect Of Erianthridin On Non-Small Cell Lung Cancer Cell Metastasis, Sutthaorn Pothongsrisit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lung cancer is one of leading cause of deaths worldwide due to cancer metastasis. The metastatic process that initiated by dissociation of cancer cells from the primary tumor and colonization of new tumor at distant organ. Cell migration and invasion play as the key early steps and required actin reorganization and extracellular matrix (ECM) degradation to achieve cell metastasis. The inhibition of these behaviors is an intriguing approach to minimize cancer metastasis. Erianthridin, a phenolic compound isolated from the Thai orchid Dendrobium formosum exhibits several pharmacological activities; however, the effect of erianthridin on lung cancer cell metastasis has not been …
Synthesis And Anti-Psoriatic Activity Of A Mycophenolic Acid-Curcumin Conjugate As A Mutual Prodrug, Yonelian Yuyun
Synthesis And Anti-Psoriatic Activity Of A Mycophenolic Acid-Curcumin Conjugate As A Mutual Prodrug, Yonelian Yuyun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Due to its several mechanisms of action, curcumin (CUR) has been employed as adjuvant therapy in treating psoriasis. Limited oral bioavailability was the problem for the development of CUR for therapeutic purposes. Therefore, many approaches, including the prodrug strategy, have been devised to address Cur's drawbacks. CUR was conjugated with mycophenolic acid (MPA) as a new prodrug in this research. Characterization of MPA-CUR structure was done by FT-IR, 1H-NMR, 13C-NMR, and MS. MPA-CUR showed water solubility at 0.73 μM and log P at 2.33, respectively. MPA-CUR was found more stable in buffer solutions than CUR and MPA. In vitro anti-psoriatic …
Surface-Enhanced Raman Scattering Using Fluorescence-Quenched Carbon Quantum Dots For Mercury Ion Detection, Andaru Dena Prasiwi
Surface-Enhanced Raman Scattering Using Fluorescence-Quenched Carbon Quantum Dots For Mercury Ion Detection, Andaru Dena Prasiwi
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mercury ion (Hg2+) is one of the most challenging problems due to its high toxicity at low concentration and bioaccumulative effects affecting human health. Herein, we propose a strategy to exploit the fluorescence quenching of carbon quantum dots (CQDs) combined with surface-enhanced Raman scattering (SERS) technique to detect Hg2+. CQDs exhibit strong fluorescence emission interfering SERS measurement. However, with the presence of Hg2+, fluorescence from CQDs can be quenched, and the SERS spectrum can be collected. In this work, SERS spectra of rhodamine 6G (R6G) incorporated with CQDs and Hg2+ (0.100‒100 ng/L) were studied. The SERS spectra of R6G was …
Development Of Protective Culture Production From Bacillus Subtilis P5-6 As Food Bio-Preservative, Hoang Truc Anh To
Development Of Protective Culture Production From Bacillus Subtilis P5-6 As Food Bio-Preservative, Hoang Truc Anh To
Chulalongkorn University Theses and Dissertations (Chula ETD)
Halophilic Bacilllus subtilis P5-6 contains bacteriocin encoding genes (subtilin and subtilosin). It has been reported as a potential strain developed to be a protective culture that could reduce viable count of Staphylococcus aureus and prolong the freshness when applied in cheese. The aim of this research was to evaluate bacteriocin generation property of P5-6 and to optimize the bacteriocin production methods for application as food bio-preservative in protective culture form. Through gene assay, only subtilosin gene expression was detected along with housekeeping gene BA-rpoB when co-cultured P5-6 with Staphylococcus aureus. The P5-6 showed low or no inhibitory effect on S. …
Paper-Based Device For Urinary Oxalate Detection, Kanphitcha Mueangdech
Paper-Based Device For Urinary Oxalate Detection, Kanphitcha Mueangdech
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this work, a simple method for urinary oxalate determination was developed using testing paper modified with reddish-brown Fe(III)-curcumin complex. Oxalate in sample interacted with Fe(III) ions on the surface of the testing paper to form a Fe(III)-oxalate complex in solution, resulting in a decrease of the amount of Fe(III)-curcumin complex on the paper surface. The amount of the remaining Fe(III)-curcumin complex on the testing paper was inversely proportional to the oxalate level in the sample solution. A change of the testing paper color from reddish-brown to yellow could be observed and the color intensity was determined by ImageJ program. …
Development Of Paper-Based Analytical Devices For Simultaneous Naked-Eye Detection Of Heavy Metal Ions, Pornphimon Kamnoet
Development Of Paper-Based Analytical Devices For Simultaneous Naked-Eye Detection Of Heavy Metal Ions, Pornphimon Kamnoet
Chulalongkorn University Theses and Dissertations (Chula ETD)
Paper-based analytical device was fabricated by a polymer screen printing and wax printing methods. The various types of device were designed for simultaneous heavy metal ions detection in synthetic unknown and real water samples. The colorimetric method was applied to detect the heavy metal ions using 1-(2-pyridylazo)-2-naphthol, bathophenanthroline, 1, 5-diphenylcarbazide, bathocuproine, dimethylglyoxime, dithizone, and 4-(2-pyridylazo) resorcinol as complexing agents. However, complexing agents require very highly selectivity. To improve the selectivity for heavy metal ion detection, a device was designed with pretreatment zones and the masking agents were applied to remove interfering ions. The proposed method was successfully applied for simultaneous …
Quantitative Trait Loci Analysis For Antioxidant Traits In Rice Oryza Sativa L., Saranyu Thaworn
Quantitative Trait Loci Analysis For Antioxidant Traits In Rice Oryza Sativa L., Saranyu Thaworn
Chulalongkorn University Theses and Dissertations (Chula ETD)
This research was conducted to study the antioxidant traits that contained the total phenolic content (TPC), total flavonoid content (TFC), and the antioxidant capacity (AC), by ABTS assay in the seed of 174 Thai rice cultivars, comprising 152 white, 10 red, and 12 purple pericarp cultivars. The hierarchical cluster analysis was evaluated, and the rice cultivars were classified into four clusters based on the antioxidant. Cluster I comprised of 72 white pericarp cultivars and had the lowest TPC, TFC, and AC. Cluster II contained 80 white cultivars and showed higher TFC values than cluster I. Cluster III consisted of 6 …
A Comparison Of Ketamine Combined With Dexmedetomidine And Ketamine Combined With Xylazine On Analgesia In Rabbits, Aomusa Kuaha
A Comparison Of Ketamine Combined With Dexmedetomidine And Ketamine Combined With Xylazine On Analgesia In Rabbits, Aomusa Kuaha
Chulalongkorn University Theses and Dissertations (Chula ETD)
The effects of ketamine combined with dexmedetomidine and ketamine combined with xylazine on analgesia and cardiopulmonary variables in rabbits were evaluated in eight healthy New Zealand White rabbits. The experiment used a randomized double-crossover design. The rabbits were randomly divided into two groups, Group KD (n=4: 2 males/2 females) receivied 35 mg kg -1of ketamine and 0.5 mg kg -1of dexmedetomidine SC, while Group KX (n=4: 2 males/2 females) receivied 35 mg kg -1of ketamine and 5 mg kg -1of xylazine SC. The physiological parameters included HR, RR, PR, SpO2, SBP and RT were determined every 5 minutes and continuously …