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Purification And Properties Of Amycolatopsis Mediterranei Dsm 43304 Lipase And Its Potential In Flavour Ester Synthesis, Dharmendra Dheeman, Gary Henehan, Jesus Maria Frias Jan 2010

Purification And Properties Of Amycolatopsis Mediterranei Dsm 43304 Lipase And Its Potential In Flavour Ester Synthesis, Dharmendra Dheeman, Gary Henehan, Jesus Maria Frias

Articles

An extracellular thermostable lipase from Amycolatopsis mediterranei DSM 43304 has been purified to homogeneity using ammonium sulphate precipitation followed by anion exchange chromatography and hydrophobic interaction chromatography. This protocol resulted in 398 fold purification with 36% final recovery. The purified A. mediterranei DSM 43304 lipase (AML) has an apparent molecular mass of 33 kDa. The N-terminal sequence, AANPYERGPDPTTASIEATR, showed highest similarity to a lipase from Streptomyces exfoliatus. The values of and for p-nitrophenyl palmitate (p-NPP) under optimal temperature (60°C) and pH (8.0) conditions were 0.10 ± 0.01 mM and 2.53 ± 0.06 mmol/minmg, respectively. The purified …


Ozone Inactivation Of Acid Stressed Listeria Monocytogenes And Listeria Innocua In Orange Juice Using A Bubble Column, Sonal Patil, Vasilis Valdramidis, Jesus Maria Frias, Patrick J. Cullen, Paula Bourke Jan 2010

Ozone Inactivation Of Acid Stressed Listeria Monocytogenes And Listeria Innocua In Orange Juice Using A Bubble Column, Sonal Patil, Vasilis Valdramidis, Jesus Maria Frias, Patrick J. Cullen, Paula Bourke

Articles

Orange juice inoculated with Listeria monocytogenes strains ATCC 7644, NCTC 11994 and Listeria innocua NCTC 11288 (106 CFU/ml) as challenge microorganisms was treated with direct ozone at 0.098mg/min/ml for different time periods (0-8 min) using an ozone bubble column. Ozone treatment of mild acid stressed and mild acid stress-habituated (pH 5.5) cells of L. monocytogenes resulted in higher inactivation times compared to control non-acid stressed cells. Additionally acid stressed cells habituated in orange juice (ATCC 7644 & NCTC 11288), showed higher inactivation times during ozonation by comparison with the control as well as the mild-acid stressed cells. Overall the …


Postharvest Hardness And Color Evolution Of White Button Mushrooms (Agaricus Bisporus)., Debabandya Mohapatra, Zuberi M. Bira, Joseph Kerry, Jesus Maria Frias, Fernanda A.R. Oliveira Jan 2010

Postharvest Hardness And Color Evolution Of White Button Mushrooms (Agaricus Bisporus)., Debabandya Mohapatra, Zuberi M. Bira, Joseph Kerry, Jesus Maria Frias, Fernanda A.R. Oliveira

Articles

The quality evaluation of mushrooms was studied by storing fresh white button mushroom (Agaricus bisporus) for 6-8 days, at various controlled temperature conditions (3.5 -15°C) and measuring the instrumental textural hardness and color of the mushroom cap for different product batches. A non linear mixed effect weibull model was used to describe mushroom cap texture and color kinetics during storage considering the batch variability into account. Storage temperature was found to play a significant role in controlling texture and colour degradation. On lowering storage temperature i) the extent of the final browning extent in the mushroom after storage …


Substrate Profiling And Aldehyde Dismutase Activity Of The Kvβ2 Subunit Of The Mammalian Kv1 Potassium Channel, A. Kumari, Barry Ryan, J. Dolly, Gary Henehan Jan 2010

Substrate Profiling And Aldehyde Dismutase Activity Of The Kvβ2 Subunit Of The Mammalian Kv1 Potassium Channel, A. Kumari, Barry Ryan, J. Dolly, Gary Henehan

Articles

Voltage-dependent potassium channels (Kv) are involved in various cellular signalling processes by governing the membrane potential of excitable cells. The cytosolic face of these α subunit-containing channels is associated with β subunits that can modulate channel responses. Surprisingly, the β subunit of the mammalian Kv1 channels, Kvβ2, has a high level of sequence homology with the aldo-keto reductase (AKR) superfamily of proteins. Recent studies have shown that Kvβ2 can catalyze the reduction of aldehydes and, most significantly, that channel function is modulated when Kvβ2-bound NADPH is concomitantly oxidized. As a result, the redox chemistry of this subunit is crucial to …


Effect Of Ultrasonic Processing On Food Enzymes Of Industrial Importance, Paula Bourke, Brijesh Tiwari, Colm O Donnell, Patrick J. Cullen Jan 2010

Effect Of Ultrasonic Processing On Food Enzymes Of Industrial Importance, Paula Bourke, Brijesh Tiwari, Colm O Donnell, Patrick J. Cullen

Articles

In the last decade power ultrasound has emerged as an alternative processing option to conventional thermal approaches for pasteurisation and sterilisation of food products. While sonication alone is not adequate for inactivation of various spoilage and harmful enzymes present in food, ultrasound in combination with mild heat treatment and/or pressure has shown potential for both enzyme and pathogen inactivation. Numerous studies have investigated ultrasound for inactivating enzymes such as pectinmethylesterase, polyphenoloxidases and peroxidases responsible for deterioration of fruit & vegetable juice and various enzymes pertinent to milk quality. The efficacy of ultrasound for the inactivation of enzymes in food is …


Rapid Microwave Synthesis Of Mesoporous Tio2 For Electrochromic Displays, Suresh Pillai, Declan Mccormack, Pradeepan Periyat, Nigel Leyland, David Corr, John Colreavy Jan 2010

Rapid Microwave Synthesis Of Mesoporous Tio2 For Electrochromic Displays, Suresh Pillai, Declan Mccormack, Pradeepan Periyat, Nigel Leyland, David Corr, John Colreavy

Articles

No abstract provided.


Enhancement Of The Antibacterial Properties Of Silver Nanoparticles Using Beta-Cyclodextrin As A Capping Agent, Swarna Jaiswal, Brendan Duffy, Niall Stobie, Patrick Mchale, Amit Jaiswal Jan 2010

Enhancement Of The Antibacterial Properties Of Silver Nanoparticles Using Beta-Cyclodextrin As A Capping Agent, Swarna Jaiswal, Brendan Duffy, Niall Stobie, Patrick Mchale, Amit Jaiswal

Articles

Silver nanoparticles (AgNPs) were synthesized by reducing silver salts using NaBH4 followed by capping with varying concentrations of β-cyclodextrin (β-CD) and were physically characterised. Antibacterial activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was determined by a microtitre well method. The AgNPs were spherical under TEM while DLS showed average diameters of capped particles to be smaller (4-7 nm) than their uncapped equivalents (17 nm). Capped particles demonstrated superior photostability when exposed to intense UV radiation for 4 hours and a significantly (p


Corrosion Protection Of Aa 2024-T3 Aluminium Alloys Using 3, 4-Diaminobenzoic Acid Chelated Zirconium-Silane Hybrid Sol-Gels, P.C. Rajath Varma, John Colreavy, John Cassidy, Mohamed Oubaha, Colette Mcdonagh, Brendan Duffy Jan 2010

Corrosion Protection Of Aa 2024-T3 Aluminium Alloys Using 3, 4-Diaminobenzoic Acid Chelated Zirconium-Silane Hybrid Sol-Gels, P.C. Rajath Varma, John Colreavy, John Cassidy, Mohamed Oubaha, Colette Mcdonagh, Brendan Duffy

Articles

Organic-inorganic polymers formed by hydrolysis/condensation reactions of alkoxide precursors, such as organically modified silanes (Ormosils) have found applications as electronic, optical and protective coatings. Such coatings possess important characteristics such as chemical stability, physical strength and scratch resistance. Further performance improvement is achieved through the incorporation of zirconium and titanium based nanoparticles, also formed through the sol-gel process. However due to the inherent difference in the reactivity of the precursors, the hydrolysis of each precursor must be carried out separately before being combined for final condensation. Zirconium precursors are commonly chelated using acetic acid or acetyl acetonate prior to hydrolysis, …


Single Step Morphology-Controlled Synthesis Of Silver Nanoparticles, Vinodkumar Etacheri, Reenamole Georgekutty, Michael Seery, Suresh Pillai Jan 2010

Single Step Morphology-Controlled Synthesis Of Silver Nanoparticles, Vinodkumar Etacheri, Reenamole Georgekutty, Michael Seery, Suresh Pillai

Articles

Silver nanoparticles having different size and plasmon resonances were synthesized through a single step aqueous based method. The current procedure was based on the reduction of silver ions by ascorbic acid in the presence of sodiumborohydride and trisodium citrate. Triangular colloidal nanoparticles having different plasmon resonances (and hence different size and colours) were synthesized by varying only the concentration of ascorbic acid. These nanoparticles were found to be stable without using any surfactants or polymers. This study revealed a strong correlation between particle growth and concentration of constituent chemicals. Crystallinity and phase purity of the silver samples were investigated through …


Media Literacy And Communication Rights: Ethical Individualism In The New Media Environment, Brian O'Neill Jan 2010

Media Literacy And Communication Rights: Ethical Individualism In The New Media Environment, Brian O'Neill

Articles

The dominant discourse of media literacy policy espouses an ethical individualism within the digital media environment in which the source of moral values and principles, and the basis of ethical evaluation, is the individual. In this perspective, even vulnerable citizens such as children and young people, who tend to be in the vanguard of new media adoption, are required to negotiate the risks and opportunities of the online world with diminishing degrees of institutional support from trusted information sources. Noticeably absent from this discourse is any consideration of the notion of communication rights. Examining an alternative conceptualization of media literacy …


Current Policy Developments In European Media Literacy, Brian O'Neill Jan 2010

Current Policy Developments In European Media Literacy, Brian O'Neill

Articles

In August of 2009, the European Commission issued a formal recommendation on media literacy adding to a series of initiatives underway since 2006 to build a comprehensive European media literacy policy (Commission of the European Communities 2009). Media literacy is now a well-established concept referring to the ability to critically understand and use media in a variety of contexts (see Aufderheide 1997). Recognising that the way people use media is changing and that the volume of information received is enormous, the Commission notes that all citizens need to have the ability to access, analyse and evaluate images, sounds and texts …


Child Sexual Abuse, Links To Later Sexual Exploitation/High-Risk Sexual Behavior, And Prevention/Treatment Programs, Kevin Lalor, Rosaleen Mcelvaney Jan 2010

Child Sexual Abuse, Links To Later Sexual Exploitation/High-Risk Sexual Behavior, And Prevention/Treatment Programs, Kevin Lalor, Rosaleen Mcelvaney

Articles

This paper reviews the literature on the nature and incidence of child sexual abuse, explores the link between child sexual abuse and later sexual exploitation, and reviews the literature on prevention strategies and effective interventions in child sexual abuse services. Our understanding of the international epidemiology of child sexual abuse is considerably greater than it was just 10 years ago, and studies from around the world are examined. Childhood sexual abuse can involve a wide number of psychological sequelae, including low self-esteem, anxiety, and depression. Numerous studies have noted that child sexual abuse victims are vulnerable to later sexual revictimization, …


Single Channel Vocal Separation Using Median Filtering And Factorisation Techniques, Derry Fitzgerald, Mikel Gainza Jan 2010

Single Channel Vocal Separation Using Median Filtering And Factorisation Techniques, Derry Fitzgerald, Mikel Gainza

Articles

This paper deals with the problem of the extraction of vocals from single channel audio signals containing both vocals and other instruments, including both pitched instruments and percussion instruments. A novel median filtering-based approach for the extraction of vocal tracks is described, which is simple and efficient to implement. Further improvements in separation quality are then obtained by the application of tensor factorisation techniques to further extract residual instruments from the vocal mix. Finally, a novel use of non-negative partial matrix cofactorisation is demonstrated as a means of further improving separation quality. Here the original single channel mixture is partially …


The Thinking Hand: Book Review, Jim Roche Jan 2010

The Thinking Hand: Book Review, Jim Roche

Articles

In this new book Juhani Pallasmaa continues his phenomenological exploration begun in ‘The Eyes of the Skin (2005)’, with the ‘Thinking Hand’ here proffered as a metaphor for his contention that all our senses, have innate imbedded crucial skills which help us perform the most basic daily tasks – and to create inspired works of art and architecture.


Site Value Tax, Tom Dunne Jan 2010

Site Value Tax, Tom Dunne

Articles

Tom Dunne discusses some of the issues surrounding property taxation in Ireland


The Construction Of Locative Situations: Locative Media And The Situationist International, Recuperation Or Redux?, Conor Mcgarrigle Jan 2010

The Construction Of Locative Situations: Locative Media And The Situationist International, Recuperation Or Redux?, Conor Mcgarrigle

Articles

A trend exists within locative media art of invoking the practices of the Situationist International (SI) as an art historical and theoretical background to contemporary practices. It is claimed that locative media seeks to re-enchant urban space though the application of locative technologies to develop novel and experimental methods for navigating, exploring and experiencing the city. To this end, SI concepts such as psychogeography and the techniques of detournement and the de ́rive (drift) have exerted considerable influence on locative media practices, but questions arise as to whether this constitutes a valid contemporary appropriation or a recuperative co-option, serving to …


Threshold Lives: Exploring The Liminal Consumption Of Tweens, Kevina Cody, Katrina Lawlor, Pauline Maclaren Jan 2010

Threshold Lives: Exploring The Liminal Consumption Of Tweens, Kevina Cody, Katrina Lawlor, Pauline Maclaren

Articles

The intervening spaces of socio-cultural organisation have proved sources of fascination and powerful theory development in the fields of sociology, psychology and anthropology (e.g. Douglas, 1966; Van Gennep, 1961; Freud, 1950, Foucault, 1977). Consumer culture research has hitherto acknowledged the potency of studying transitional phenomena, spaces and places and the interactions of varied ontologies with the consumption experiences of the individuals or group in flux and evolution (e.g. Davies and Fitchett, 2004; Maldonado and Tansuhaj, 1999; Gentry, 1997; Schouten, 1991). But what of those whose reality is the threshold between two ordered and defined worlds, the centre of nowhere? This …


Simulation Optimisation Methods In Supply Chain Applications: A Review, Amr Arisha, Waleed Abo-Hamad Jan 2010

Simulation Optimisation Methods In Supply Chain Applications: A Review, Amr Arisha, Waleed Abo-Hamad

Articles

The competitiveness and dynamic nature of today’s marketplace is due to rapid advances in information technology, short product life cycles and the continuing trend in global outsourcing. Managing the resulting supply chain networks effectively is challenged by high levels of uncertainty in supply and demand, confl ict objectives, vagueness of information, numerous decision variables and constraints. With such levels of complexity, supply chain optimisation has the potential to make a signifi cant contribution in resolving these challenges. In this paper, a literature review – based on more than 100 peer-reviewed articles – of state-of-the-art simulation-based optimisation techniques in the context …


Living Separate And Apart: Solving The Problem Of Putative Community Property In Louisiana, Casey E. Faucon Jan 2010

Living Separate And Apart: Solving The Problem Of Putative Community Property In Louisiana, Casey E. Faucon

Articles

No abstract provided.


Bailouts And Credit Cycles: Fannie, Freddie, And The Farm Credit System, Julie Andersen Hill Jan 2010

Bailouts And Credit Cycles: Fannie, Freddie, And The Farm Credit System, Julie Andersen Hill

Articles

In September 2008 the United States government seized mortgage giants Fannie Mae and Freddie Mac Since that time the government has pumped 111 billion of new capital into these governmentsponsored enterprises Yet the future of these companies postbailout is far from clear As policymakers consider the future of Fannie and Freddie it is useful to remember that this is not the first significant bailout of a governmentsponsored enterprise The government also rescued the Farm Credit System in the 1980s This Article examines the historical cycles in which Fannie Freddie and the Farm Credit System have funded loans They fund more …


Living History Interview Comment, Richard Delgado, Jean Stefancic Jan 2010

Living History Interview Comment, Richard Delgado, Jean Stefancic

Articles

No abstract provided.


Gender Rules, Meredith M. Render Jan 2010

Gender Rules, Meredith M. Render

Articles

Sex stereotypes are of perennial concern within antidiscrimination law and theory, yet there is widespread disagreement about what constitutes a "sex stereotype." This Article enters the debate surrounding the correct understanding of "stereotype" and posits that the concept is too thin to serve as a criterion for distinguishing "discriminatory" gender generalizations from non-discriminatory, probabilistic descriptions of behavior. Instead, "stereotype" is a heuristic that has been used by courts and commentators to crudely capture judgments about the justness of applying sex-respecting rules. In this light, the Article argues that the stereotype heuristic should be abandoned in favor of a rule-centered analysis …


The Argot Of Equality: On The Importance Of Disentangling Diversity And Remediation As Justifications For Race-Conscious Government Action, Ronald J. Krotoszynski Jr. Jan 2010

The Argot Of Equality: On The Importance Of Disentangling Diversity And Remediation As Justifications For Race-Conscious Government Action, Ronald J. Krotoszynski Jr.

Articles

The rules governing "benign" forms of race-conscious government action are easy to state but very difficult to apply in practice. A great deal of the difficulty arises from the lack of precision associated with the use of terms of art, such as "diversity," "remediation," and "affirmative action." Each of these terms should have a concrete and separate meaning, but in reality often serve as mere synonyms; this lack of precision in nomenclature is not always accidental. Although broad majorities support efforts to increase "diversity," race-conscious government action aimed at remediating past racial discrimination enjoys much more limited popular support. The …


The Shot (Not) Heard Round The World: Reconsidering The Perplexing U.S. Preoccupation With The Separation Of Legislative And Executive Powers, Ronald J. Krotoszynski Jr. Jan 2010

The Shot (Not) Heard Round The World: Reconsidering The Perplexing U.S. Preoccupation With The Separation Of Legislative And Executive Powers, Ronald J. Krotoszynski Jr.

Articles

Since the drafting of the U.S. Constitution in 1787, the document has served as a model for constitutional design for many other democratic polities. Core elements of U.S. constitutionalism, including adoption of a written constitution, entrenched and judicially enforceable human rights, and federalism, have become commonplace in other nations' constitutions. One key element of U.S. constitutional structure, however, has failed to find a receptive audience abroad: the separation of legislative and executive powers. Most modern democracies have broken with the British model of parliamentary supremacy in favor of some system of judicial enforcement of entrenched human rights, but nevertheless have …


Talk The Talk, But Walk The Walk: A Comment On Joan Williams's Reshaping The Work-Family Debate Crowdsourcing The Work-Family Debate: A Colloquy, Jean Stefancic Jan 2010

Talk The Talk, But Walk The Walk: A Comment On Joan Williams's Reshaping The Work-Family Debate Crowdsourcing The Work-Family Debate: A Colloquy, Jean Stefancic

Articles

No abstract provided.


Rodrigo's Reconsideration: Intersectionality And The Future Of Critical Race Theory, Richard Delgado Jan 2010

Rodrigo's Reconsideration: Intersectionality And The Future Of Critical Race Theory, Richard Delgado

Articles

No abstract provided.


Transparency, Accountability, And Competency: An Essay On The Obama Administration, Google Government, And The Difficulties Of Securing Effective Governance Symposium: What Change Will Come: The Obama Administration And The Future Of The Administrative State, Ronald J. Krotoszynski Jr. Jan 2010

Transparency, Accountability, And Competency: An Essay On The Obama Administration, Google Government, And The Difficulties Of Securing Effective Governance Symposium: What Change Will Come: The Obama Administration And The Future Of The Administrative State, Ronald J. Krotoszynski Jr.

Articles

No abstract provided.


Freedom Meador Lecture Series 2009-2010: Freedom, Kenneth M. Rosen Jan 2010

Freedom Meador Lecture Series 2009-2010: Freedom, Kenneth M. Rosen

Articles

No abstract provided.


Predatory Bundling And The Exclusionary Standard, J. Shahar Dillbary Jan 2010

Predatory Bundling And The Exclusionary Standard, J. Shahar Dillbary

Articles

Recent decisions--all relying on a stylized example first provided by the Ortho court--hold that a multi-product seller that uses a bundled discount in a way that excludes an equally or more efficient competitor engages in predatory bundling. According to these decisions, a bundle can be considered “predatory” even when the price of the bundle exceeds its cost. This Article shows that the Ortho court's stylized example and its monopoly leveraging theory are erroneous. This Article further demonstrates that even when a bundle's price excludes more efficient competitors and even when a component in the bundle is priced below cost, and …


Public Interest(S) And Fourth Amendment Enforcement, Alexander A. Reinert Jan 2010

Public Interest(S) And Fourth Amendment Enforcement, Alexander A. Reinert

Articles

Fourth Amendment events generate substantial controversy among the public and in the legal community. Yet there is orthodoxy to Fourth Amendment thinking, reflected in the near universal assumption by courts and commentators alike that the amendment creates only tension between privately held individual liberties and public-regarding interests in law enforcement and security. On this account, courts are faced with a clear choice when mediating Fourth Amendment conflicts: side with the individual by declaring a particular intrusion to be in violation of the Constitution or side with the public by permitting the intrusion. Scholarly literature and court decisions are accordingly littered …