Digital Commons Network^™

The Potential Of Cold Plasma For Safe And Sustainable Food Production, Paula Bourke, Dana Ziuzina, Daniela Boehm, Patrick J. Cullen, Kevin Keener

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In-package decontamination of foods using cold plasma has advanced this technology as a unit process for fresh foods decontamination and shelf-life extension.

Chemical residues of agricultural pesticides of varying structure can be degraded to safe or less-toxic structures using cold plasma.

Cold-plasma-mediated control of contaminants, along with the promotion of seed germination and plant growth, offers alternatives to current pesticides and fertilizers for agriculture.

Controlling plasma reactive species formulations in dry and liquid delivery formats advances the potential for understanding and successful translation to multiple points along the agriculture and food sectors.

Employing predictive microbiology, process optimization tools and a …

Patient Experiences Of A Physiotherapy-Led Multidisciplinary Rehabilitative Intervention After Successful Treatment For Oesophago-Gastric Cancer, Annemarie Bennett, L. O'Neill, D. Connolly, E. M. Guinan, L. Boland, Suzanne Doyle, J. O'Sullivan, J. V. Reynolds, J. Hussey

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Purpose To qualitatively explore the perceived impact of a 12-week rehabilitative intervention for oesophago-gastric cancer survivors on their physical, mental and social wellbeing. Methods Of the 21 participants who completed the intervention, 19 took part in a semi-structured focus group interview. Four audio-taped focus groups were held, ranging in size from two to eight participants. Focus groups were transcribed and analysed using a descriptive qualitative approach. Results At recruitment, participants were 23.5 ± 15.2 months post-surgery and all had suboptimal fitness levels. Participants reported improvements in their physical capacity and ability to carry out activities of daily living during the …

Optimization Of Folic Acid Supplementation In The Prevention Of Neural Tube Defects, Shona Cawley, Daniel Mccartney, Jayne V. Woodside, Mary Rose Sweeney, Robert Mcdonnell, Anne M. Molloy, Michael J. Turner

Articles

Background We examined the relationship between timing and duration of folic acid (FA) supplementation in achieving red blood cell (RBC) folate levels in early pregnancy which are optimal (>906 nmol/l) for the prevention of neural tube defects (NTDs). Methods Clinical, FA supplementation and dietary folate details were computerized at the first antenatal visit. Maternal blood samples were analysed for RBC and serum folate. Results Of the 502 women, 98.2% (n = 493) reported taking FA. There was a positive correlation between duration of supplementation and both RBC folate (r = 0.43, P < 0.001) and serum folate (rho = 0.29, P < 0.001). The optimal RBC folate level was achieved in 80.4% (n = 46) of women who started FA 400 μg 4–8 weeks before their LMP compared with only 53.6% (n = 153) in women who started 4–8 weeks after their LMP (P < 0.001). Conclusions This study provides, for the first …

Microfluidics In Coagulation Monitoring Devices: A Mini Review, Leanne Harris, Anthony J. Killard

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Diagnosis and monitoring of disorders of coagulation and disturbances in haemostasis has been around for many years. Thrombotic and bleeding disorders are amongst the leading causes of mortality in the developed world, thus driving research in this area. The simplicity with which miniaturised devices operate and their application to the study of coagulation and haemostasis offer potential advantages over traditional testing, such as improvements in clinical decision making and ultimately patient care. This review looks at progress in microfluidic device development in the field of haemostasis and coagulation biology.

Comparison At The First Prenatal Visit Of The Maternal Dietary Intakes Of Smokers With Non-Smokers In A Large Maternity Hospital: A Cross-Sectional Study, Emer G. O'Malley, Shona Cawley, Ciara M.E. Reynolds, Rachel A.K. Kennedy, Anne Molloy, Michael Turner

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Using detailed dietary and supplement questionnaires in early pregnancy, we compared the dietary intakes of micronutrients and macronutrients at the first prenatal visit of women who reported continuing to smoke during pregnancy with the intakes of women who were non-smokers.

Raman Spectroscopic Analysis Of High Molecular Weight Proteins In Solution - Considerations For Sample Analysis And Data Pre-Processing, Drishya Rajan Parachalil, Brenda B. Brankin, Jennifer Mcintyre, Hugh Byrne

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This study explores the potential of Raman spectroscopy, coupled with multivariate regression techniques and a protein separation technique (ion exchange chromatography), to quantitatively monitor diagnostically relevant changes in high molecular weight proteins in liquid plasma. Measurement protocols to detect the imbalances in plasma proteins as an indicator of various diseases using Raman spectroscopy are optimised, such that strategic clinical applications for early stage disease diagnostics can be evaluated. In a simulated plasma protein mixture, concentrations of two proteins of identified diagnostic potential (albumin and fibrinogen) were systematically varied within physiologically relevant ranges. Scattering from the poorly soluble fibrinogen fraction is …

Awareness And Prevalence Of Human Milk Sharing And Selling In The United States, Elizabeth O'Sullivan, Sheelagh R. Geraghty, Kathleen Maher Rasmussen

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There are limited data available about the prevalence of human milk (HM) sharing and selling in the general population. We aimed to describe attitudes toward HM selling among participants in a qualitative‐interview study and prevalence of HM sharing and selling among a national sample of U.S. mothers. Mothers (n = 41) in our qualitative‐interview study felt that sharing or donating HM was more common than selling; none had ever purchased or sold HM. Three themes related to HM selling emerged from this work: questioning the motives of those selling HM, HM selling limits access to HM to those with money, …

Assessment Of The Disinfection Capacity And Eco-Toxicological Impact Of Atmospheric Cold Plasma For Treatment Of Food Industry Effluents, Apurva Patange, Daniela Boehm, Michelle Giltrap, Peng Lu, Patrick J. Cullen, Paula Bourke

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Generation of wastewater is one of the main environmental sustainability issues across food sector industries. The constituents of food process effluents are often complex and require high energy and processing for regulatory compliance. Wastewater streams are the subject of microbiological and chemical criteria, and can have a significant eco-toxicological impact on the aquatic life. Thus, innovative treatment approaches are required to mitigate environmental impact in an energy efficient manner. Here, dielectric barrier discharge atmospheric cold plasma (ACP) was evaluated for control of key microbial indicators encountered in food industry effluent. This study also investigated the eco-toxicological impact of cold plasma …

“High” Standards: How The Tide Of Marijuana Legalization Sweeping The Country Ignores The Hidden Risks Of Edibles, Steve Calandrillo, Katelyn Fulton

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As a tide of marijuana legalization sweeps across the United States, there is a surprising lack of scrutiny as to whether the benefits of recreational marijuana outweigh the risks. Notably, marijuana edibles present special risks to the population that are not present in smoked marijuana. States that have legalized recreational marijuana are seeing an increase in edible-related calls to poison control centers and visits to emergency rooms. These negative reactions are especially prevalent in vulnerable populations such as children, persons with underlying preexisting conditions, and out-of-state marijuana novices. Unfortunately, research on edible marijuana is scant and state regulatory regimes are …

Ph-Dependent Silica Nanoparticle Dissolution And 1 Cargo Release, Giorgia Giovaninni, Colin J. Moore, Andrew John Hall, Hugh Byrne, Vladimir Gubala

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The dissolution of microporous silica nanoparticles (NP) in aqueous environments of different biologically relevant pH was studied in order to assess their potential as drug delivery vehicles. Silica NPs, loaded with fluorescein, were prepared using different organosilane precursors (tetraethoxysilane, ethyl triethoxysilane or a 1:1 molar ratio of both) and NP dissolution was evaluated in aqueous conditions at pH 4, pH 6 and pH 7.4. These conditions correspond to the acidity of the intracellular environment (late endosome, early endosome, cytosol respectively) and gastrointestinal tract (‘fed’ stomach, duodenum and jejunum respectively). All NPs degraded at pH 6 and pH 7.4, while no …

Application Of Box-Behnken Experimental Design For The Formulation And Optimisation Of Selenomethionine-Loaded Chitosan Nanoparticles Coated With Zein For Oral Delivery., Giuliana Vozza, Hugh Byrne, Jesus Maria Frias, Minna Khalid Danish, Sinéad M. Ryan

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Selenomethionine is an essential amino acid with a narrow therapeutic index and susceptibility to oxidation. Here it was encapsulated into a nanoparticle composed of chitosan cross-linked with tripolyphosphate for oral delivery. The formulation was optimised using a three-factor Box-Behnken experimental design. The chitosan:tripolyphosphate ratio, chitosan solvent pH, and drug load concentration were independently varied. The dependent variables studied were encapsulation efficiency, particle size, polydispersity index and zeta potential. For optimisation, encapsulation efficiency and zeta potential were maximised, particle diameter was set to 300 nm and polydispersity index was minimised. A 0.15 mg/mL concentration of selenomethionine, chitosan solvent pH of 3, …

Quantifying The Concentration Of Glucose, Urea, And Lactic Acid In Mixture By Confocal Raman Microscopy, Zhengyuan Tang, Sinead Barton, Thomas Ward, John Lowry, Michelle Doran, Hugh Byrne, Bryan Hennelly

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Raman spectroscopy has numerous applications in the field of biology. One such application is the simultaneously measurement of the concentration of multiple biochemical components in low volume aqueous mixtures, for example, a single drop of blood serum. Over twenty years ago, it was shown for the first time that it was possible to estimate the concentration of glucose, urea, and lactic acid in mixture by combining Raman Spectroscopy with Partial Least Squares Regression analysis. This was followed by numerous contributions in the literature designed to increase the number of components and reduce the limits of concentration that could be simultaneously …

Inner Surface Biofilm Inactivation By Atmospheric Pressure Helium Porous Plasma Jet, Peng Lu, Dana Ziuzina, Patrick J. Cullen, Paula Bourke

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We present a helium porous plasma jet based on gas diffuser, designed with the aim of decontaminating inner surfaces of contaminated structures, e.g., pipes. The porous plasma jet operates with three discharge modes in ambient air and inside a vessel. Its decontamination capacity is demonstrated by evaluating the inactivation efficacy of biofilm form of Pseudomonas aeruginosa, adherent to inner surface of a glass vial. Plasma treatments for 5 min with filament mode and double region helium discharge mode reduced bacterial numbers by 2.4 and 2.5 Log₁₀ CFU/ml. Plasma treatment with double region air‐like discharge mode was the most …

Blunted Serum 25(Oh)D Response To Vitamin D3 Supplementation In Children With Autism, Conor Kerley, Basil Elnazir, Peter Greally, David Coghlan

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Introduction: Data suggest a potential role for vitamin D in autism spectrum disorder (ASD) prevention and treatment. It is likely that the serum response to vitamin D supplementation contributes to its effectiveness. Multiple factors affect serum vitamin D 25(OH)D response to supplementation.

Methods: We conducted post-hoc analysis of two double-blind, randomized, placebo-controlled trials (RCT) of vitamin D3 supplementation, one RCT involving children with ASD and another involving children with asthma. Both trials were conducted in the same geographic location (Dublin, Ireland, 53°N), conducted over Winter season and utilized the same vitamin D3 dose (2000 IU/day).

Results: We …

Sarcopenia During Neoadjuvant Therapy For Oesophageal Cancer: Characterising The Impact On Muscle Strength And Physical Performance, E.M. Guinan, Suzanne Doyle, A.E. Bennett, L. O'Neill, J. Gannon, J.A. Elliott, J. O'Sullivan, J.V. Reynolds, J. Hussey

Articles

Purpose Preoperative chemo(radio)therapy for oesophageal cancer (OC) may have an attritional impact on body composition and functional status, impacting postoperative outcome. Physical decline with skeletal muscle loss has not been previously characterised in OC and may be amenable to physical rehabilitation. This study characterises skeletal muscle mass and physical performance from diagnosis to post-neoadjuvant therapy in patients undergoing preoperative chemo(radio)therapy for OC.

Methods Measures of body composition (axial computerised tomography), muscle strength (handgrip), functional capacity (walking distance), anthropometry (weight, height and waist circumference), physical activity, quality-of-life and nutritional status were captured prospectively. Sarcopenia status was defined as pre-sarcopenic (low muscle …

Mitochondrial Dna Replacement: Moral And Halakhic Concerns, J. David Bleich

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Mitochondrial DNA (mtDNA), transmitted from mother to child, have their own genetic code that may cause debilitating genetic diseases. To prevent such unfortunate occurrences, researchers have developed a process enabling them to completely replace an ovum’s mitochondria with mitochondria contributed by a donor. Children born by use of this method have genetic material from both the mitochondrial donor and the birth mother; they are “three-parent babies.” Resultant medical, ethical, legal and theological problems are obvious.

Moreover, this technology may pose significant risks to neonates born of such procedures. Certainly no person has the right to cause harm to a fellow …

Energy Efficient Hybrid Routing Protocol Based On The Artificial Fish Swarm Algorithm And Ant Colony Optimisation For Wsns, Xinlu Li, Brian Keegan, Fredrick Mtenzi

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Wireless Sensor Networks (WSNs) are a particular type of distributed self-managed network with limited energy supply and communication ability. The most significant challenge of a routing protocol is the energy consumption and the extension of the network lifetime. Many energy-efficient routing algorithms were inspired by the development of Ant Colony Optimisation (ACO). However, due to the inborn defects, ACO-based routing algorithms have a slow convergence behaviour and are prone to premature, stagnation phenomenon, which hinders further route discovery, especially in a large-scale network. This paper proposes a hybrid routing algorithm by combining the Artificial Fish Swarm Algorithm (AFSA) and ACO …

Relating Group Size And Posting Activity Of An Online Community Of Financial Investors: Regularities And Seasonal Patterns, P. Racca, R. Casarin, Pierpaolo Dondio, F. Squazzoni

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Group size can potentially affect collective activity and individual propensity to contribute to collective goods. Mancur Olson, in his Logic of Collective Action, argued that individual contribution to a collective good tends to be lower in groups of large size. Today, online communication platforms represent an interesting ground to study such collaborative dynamics under possibly different conditions (e.g., lower costs related to gather and share information). This paper examines the relationship between group size and activity in an online financial forum, where users invest time in sharing news, analysis and comments with other investors. We looked at about 24 million …

Examining The Effects Of A Virtual Character On Learning And Engagement In Serious Games, Vihanga Gamage, Cathy Ennis

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Virtual characters have been employed for many purposes including interacting with players of serious games, with a purpose to increase engagement. These characters are often embodied conversational agents playing diverse roles, such as demonstrators, guides, teachers or interviewers. Recently, much research has been conducted into properties that affect the realism and plausibility of virtual characters, but it is less clear whether the inclusion of interactive agents in serious applications can enhance a user’s engagement with the application, or indeed increase efficacy. In a first step towards answering these questions, we conducted a study where a Virtual Learning Environment was used …

Nano-Strategies To Fight Multidrug Resistant Bacteria—“A Battle Of The Titans", Pedro V. Baptista, Matthew P. Mccusker, Andreia Carvalho, Daniela A. Ferreira, Niamh M. Mohan, Marta Martins, Alexandra R. Fernandez

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Infectious diseases remain one of the leading causes of morbidity and mortality worldwide. The WHO and CDC have expressed serious concern regarding the continued increase in the development of multidrug resistance among bacteria. Therefore, the antibiotic resistance crisis is one of the most pressing issues in global public health. Associated with the rise in antibiotic resistance is the lack of new antimicrobials. This has triggered initiatives worldwide to develop novel and more effective antimicrobial compounds as well as to develop novel delivery and targeting strategies. Bacteria have developed many ways by which they become resistant to antimicrobials. Among those are …

Unprecedented In Vitro Antitubercular Activitiy Of Manganese(Ii) Complexes Containing 1,10- Phenanthroline And Dicarboxylate Ligands: Increased Activity, Superior Selectivity, And Lower Toxicity In Comparison To Their Copper(Ii) Analogs, Pauraic Mccarron, Malachy Mccann, Michael Devereux, Kevin Kavanagh, Ciaran M. Skerry, Petros C. Karakousis, Ana Carolina Aor, Thaís P. Mello, Dos Santos, André Luis Souza Dos Santos, André Luis Souza, Débora Leite Campos, Fernando R. Pavan

Articles

Mycobacterium tuberculosis is the etiologic agent of tuberculosis. The demand for new chemotherapeutics with unique mechanisms of action to treat (multi)resistant strains is an urgent need. The objective of this work was to test the effect of manganese(II) and copper(II) phenanthroline/dicarboxylate complexes against M. tuberculosis. The water-soluble Mn(II) complexes, [Mn2(oda)(phen)4(H2O)2][Mn2(oda)(phen)4(oda)2]·4H2O (1) and ([Mn(3,6,9-tdda)(phen)2]·3H2O·EtOH)n (3) (odaH2 = octanedioic acid, phen = 1,10-phenanthroline, tddaH2 = 3,6,9-trioxaundecanedioic acid), and water-insoluble complexes, [Mn(ph)(phen)(H2O)2] (5), [Mn(ph)(phen)2(H2O)]·4H2O (6), [Mn2(isoph)2(phen)3]·4H2O (7), ([Mn(phen)2(H2O)2])2(isoph)2(phen)·12H2O (8) and [Mn(tereph)(phen)2]·5H2O (9) (phH2 = phthalic acid, isophH2 = isophthalic acid, terephH2 = terephthalic acid), robustly inhibited the viability of M. tuberculosis strains, H37Rv …

Quality Of Fresh And Stored Mares’ Milk, Grażyna Czyżak-Runowska, Jacek Wójtowski, Alicja Niewiadomska, Maria Markiewicz-Keszycka

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Mares’ milk is characterised by unique nutritional profile. In this study, the microbiological analysis of mares’ milk was performed. The presence of total bacteria, total lactic bacteria, Lactobacillus spp., Streptococcus lactis, Salmonella spp. and coliforms was investigated. Moreover, the influence of refrigerated and frozen storage on the total bacteria count, vitamin C, acidity and colour of milk was examined. Pathogenic Salmonella spp. and coliforms were not detected in the raw milk. It was revealed that mares’ milk can be stored for 72 hours under refrigeration at a temperature of +4 °C without reducing its microbiological quality. Most of the physicochemical …

Significance Of A Non-Thermal Plasma Treatment On Ldpe Biodegradation With Pseudomonas Aeruginosa, Laurence Scally, Miroslav Gulan, Lars Weigang, Vladimir Milosavljević, Patrick J. Cullen

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The use of plastics has spanned across almost all aspects of day to day life. Although their uses are invaluable, they contribute to the generation of a lot of waste products that end up in the environment and end up polluting natural habitats such as forests and the ocean. By treating low-density polyethylene (LDPE) samples with non-thermal plasma in ambient air and with an addition of ≈4% CO2, the biodegradation of the samples can be increased due to an increase in oxidative species causing better cell adhesion and acceptance on the polymer sample surface. It was, however, found that the …

Hydrogen Peroxide And Beyond - The Potential Of High-Voltage Plasma Activated Liquids Against Cancerous Cells, Daniela Boehm, James Curtin, Patrick J. Cullen, Paula Bourke

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Abstract: The use of plasma-activated liquids such as PBS, medium or simply plasma-activated water (PAW) has been receiving increasing attention for applications in cancer treatments. Amongst the reactive species contained in these solutions, hydrogen peroxide appears to play a pivotal role in causing cytotoxic effects. While H2O2 concentrations can be correlated with reduced cell viability and growth and used as an indicator of the potential efficacy of a plasma-activated water, comparisons to standard H2O2 kill curves demonstrate a potency in PAW which exceeds H2O2 associated toxicity, indicating that other plasma-generated species play an important role. Using a high-voltage dielectric barrier …

Special Issue On The Use Of Accurate Tissue And Body Models In Rf And Mw Medical Applications, Giuseppe Ruvio, Marta Cavagnaro

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No abstract provided.

Chlorate And Other Oxychlorine Contaminants Within The Dairy Supply Chain, William P. Mccarthy, Tom F. O'Callaghan, Martin Danaher, David Gleeson, Christine O'Connor, Mark A. Fenelon, John Tobin

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The presence of chlorate in milk and dairy products can arise from the use of chlorinated water and chlorinated detergents for cleaning and sanitation of process equipment at both farm and food processor level. Chlorate and other oxychlorine species have been associated with inhibition of iodine uptake in humans and the formation of methemoglobin, with infants and young children being a high‐risk demographic. This comprehensive review of chlorate and chlorine derivatives in dairy, highlights areas of concern relative to the origin and/or introduction of chlorate within the dairy supply chain. This review also discusses the associated health concerns, regulations, and …

Hydra As A Model For Screening Ecotoxicological Effects Of Plasma-Treated Water, Dana Ziuzina, Chaitanya Sarangapani, Daniela Boehm, Paula Bourke

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Atmospheric cold plasma (ACP) has been widely researched to generate functionalized solutions for decontamination of liquids and wastewater treatment. The present investigation demonstrates that Hydra can be used as an additional in vivo tool to monitor the impact of plasma-processed solutions on the aquatic environment.

The N-Acyltransferase Lnt: Structure-Function Insights From Recent Simultaneous Studies, Wei Chang, Declan A. Doyle, Toufic El Arnaout

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Bacterial lipoproteins have been researched for decades due to their roles in a large number of biological functions. There were no structures of their main three membrane processing enzymes, until 2016 for Lgt and LspA, and then 2017 for Lnt with not one but three simultaneous, independent publications. We have analyzed the recent findings for this apolipoprotein N-acyltransferase Lnt, with comparisons between the novel structures, and with soluble nitrilases, to determine the significance of unique features in terms of substrate's recognition and binding mechanism influenced by exclusive residues, two transmembrane helices, and a flexible loop.

Targeting The Folate Receptor: Improving Efficacy In Inorganic Medicinal Chemistry, Pauraic Mccarron, Aisling Crowley, Denis O'Shea, Malachy Mccann, Orla L. Howe, Mary Hunt, Michael Devereux

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The discovery of the high-affinity, high-specificity folate receptor in mamalian kidney cells, coupled with the ability of folate to enter cells by folate receptor-mediated endocytosis and the subsequent elucidation of the folate receptor’s overexpression in specific cancer cell types; heralded the arrival of the area of chemotherapeutic folate targeting. The application of purely organic folate-based small-molecule drug conjugates that selectively target the folate receptor, which is over expressed in several diseases such as cancer, is well established. The application of inorganic folate-targeted drugs offers significant potential to expand and enhance this therapeutic approach. From the data made available to date, …

Smokers And Ex-Smokers Have Shared Differences In The Neural Substrates For Potential Monetary Gains And Losses, Liam Nestor, Ella Mccabe, Jennifer Jones, Luke Clancy, Hugh Garavan

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Despite an increased understanding of nicotine addiction, there is a scarcity of research comparing the neural correlates of non-drug reward between smokers and ex-smokers. Long-term changes in reward-related brain functioning for non-drug incentives may elucidate patterns of functioning that potentially contribute to ongoing smoking behaviour in current smokers. Similarly, examining the effects of previous chronic nicotine exposure during a period of extended abstinence may reveal whether there are neural correlates responsible for non-drug reward processing that are different from current smokers.