Digital Commons Network^™

Co-Colonisation With Aspergillus Fumigatus And Pseudomonas Aeruginosa Is Associated With Poorer Health In Cystic Fibrosis Patients: An Irish Registry Analysis, Emma Reece, Ricardo Segurado, Abaigeal Jackson, Siobhan Mcclean, Julie Renwick, Peter Greally

Articles

Pulmonary infection is the main cause of death in cystic fibrosis (CF). Aspergillus fumigatus (AF) and Pseudomonas aeruginosa (PA) are the most prevalent fungal and bacterial pathogens isolated from the CF airway, respectively. Our aim was to determine the effect of different colonisation profiles of AF and PA on the clinical status of patients with CF.

The Influence Of Match Outcome On Running Performance In Elite Gaelic Football, Shane Mangan, Shane Malone, Martin Ryan, Jason Mcgahan, Cian O'Neill, Con Burns, Joe Warne, Denise Martin, Kieran Collins

Articles

Introduction: Match outcome has been linked to running performance in many sports; however, the association has not yet been examined in Gaelic football. Methods: GPS technologies (4-Hz, VX Sport, Lower Hutt, New Zealand) were used with 5 elite Gaelic football teams over a period of 5 years (2012–2016), amounting to 95 matches. In total, 780 full match data sets were collected. Game movements were divided into total distance and high-speed distance (≥17 km h−1). Match outcomes were divided into big losses (≥6 points), small losses (≤5points), draws, small wins (≤5 points) and big wins (≥6 points). Results: Team (P …

The Electrochemical Deposition Of Zn-Mn Coating From Choline Choloride-Urea Deep Eutectic Solvent, Mihael Bucko, David Culliton, Tony Betts, Jelena Bajat

Articles

Electrochemical and microscopic techniques were used for characterization of Zn˗Mn coatings electrodeposited from choline chloride˗urea deep eutectic solvent. Cyclic voltammograms show that there was no discernible Mn reduction peak when only Mn2+ was present in DES solution. The distinct Mn peak developed only upon addition of Zn2+ to the solution, probably due to previous Zn nucleation on the steel substrate. It was found that 22-27 wt.% Mn, was deposited at current densities of 3-8 mA cm-2, amounts significantly higher than in aqueous electrolytes. Since higher deposition current densities resulted in the formation of a porous surface consisting of clusters of …

Doxorubicin Kinetics And Effects On Lung Cancer Cell Lines Using In-Vitro Raman Micro-Spectroscopy: Binding Signatures, Drug Resistance And Dna Repair, Hugh Byrne, Franck Bonnier, Alan Casey, Orla L. Howe, Zeineb Farhane

Articles

Raman micro-spectroscopy is a non-invasive analytical tool, whose potential in cellular analysis and monitoring drug mechanisms of action has already been demonstrated, and which can potentially be used in pre-clinical and clinical applications for the prediction of chemotherapeutic efficacy. To further investigate such potential clinical application, it is important to demonstrate its capability to differentiate drug mechanisms of action and cellular resistances. Using the example of Doxorubicin (DOX), in this study, it was used to probe the cellular uptake, signatures of chemical binding and subsequent cellular responses, of the chemotherapeutic drug in two lung cancer cell lines, A549 and Calu-1. …

Vibrational Spectroscopy As A Tool For Studying Drug-Cell Interaction: Could High Throughput Vibrational Spectroscopic Screening Improve Drug Development, Lauren E. Jamieson, Hugh Byrne

Articles

Vibrational spectroscopy is currently widely explored as a tool in biomedical applications. An area at the forefront of this field is the use of vibrational spectroscopy for disease diagnosis, ultimately aiming towards spectral pathology. However, while this field shows promising results, moving this technique into the clinic faces the challenges of widespread clinical trials and legislative approval. While spectral pathology has received a lot of attention, there are many other biomedical applications of vibrational spectroscopy, which could potentially be translated to applications with greater ease. A particularly promising application is the use of vibrational spectroscopic techniques to study the interaction …

Prediction Of Viral Loads For Diagnosis Of Hepatitis C Infection In Human Plasma Samples Using Raman Spectroscopy Coupled With Partial Least Squares Regression Analysis, Hugh Byrne, Franck Bonnier, H. Nawaz, N. Rashid, M. Saleem

Articles

Raman spectroscopy has been used to identify the biochemical changes associated with the presence of the Hepatitis C virus (HCV) in infected human blood plasma samples as compared with healthy samples, as control. The aim of the study was to establish the Raman spectral markers of hepatitis infection, which could be used for diagnostic purposes. Moreover, multivariate data analysis techniques, including Principal Component Analysis (PCA), coupled with Linear Discriminant Analysis (LDA), and Partial Least Square Regression (PLSR) are employed to further demonstrate the diagnostic capability of the technique. The PLSR model is developed to predict the viral loads of the …

Linking Atr-Ftir And Raman Features To Phenolic Extractability And Other Attributes In Grape Skin, Hugh Byrne, Abigail Rooney, Julio Nogales-Bueno, Jose Miguel Hernandez-Hierro, Francisco Jose Heredia

Articles

The importance of wine phenolics on the sensory characteristic of red wines is well-known. Therefore, it is necessary to control the extractability of phenolic compounds from grape skins, which depends significantly on grape ripeness and hence, on cell wall degradation.

In the present study, attenuated total reflectance Fourier transform infrared (ATR-FTIR) and Raman spectra of grape skin have been recorded. Then, these spectral matrices have been studied and the main spectral features have been linked to extractabilities of phenolic compounds (anthocyanins, flavanols and total phenols). Moreover, spectral differences between external and internal grape skin surfaces also have been studied.

It …

Determination Of Spectral Markers Of Cytotoxicity And Genotoxicity Using In Vitro Raman Microspectroscopy: Cellular Responses To Polyamidoamine Dendrimer Exposure, Esen Efeoglu, Alan Casey, Hugh Byrne

Articles

Although consumer exposure to nanomaterials is ever increasing, with potential increased applications in areas such as drug and/or gene delivery, contrast agents and diagnosis, determination of cyto- and geno- toxic effect of nanomaterials on human health and the environment still remains challenging. Although many techniques have been established and adapted to determine the cytotoxicity and genotoxicity of nano-sized materials, these techniques remain limited by the number of assays required, total cost, use of labels and they struggle to explain the underlying interaction mechanisms. In this study, Raman microspectroscopy is employed as an in vitro label free high content screening technique …

A Natural, Calcium-Rich Marine Multi-Material Complex Preserves Bone Structure, Composition And Strength In An Ovariectomized Rat Model Of Osteoporosis, Orlaith Brennan, Joseph Sweeney, Brian O'Meara, Amro Widaa, Franck Bonnier, Hugh Byrne, Denis O'Gorman, Fergal O'Brien

Articles

Calcium supplements are used as an aid in the prevention of osteopenia and osteoporosis and also for the treatment of patients when used along with medication. Many of these supplements are calcium carbonate based. This study compared a calcium-rich, marine multi-mineral complex (Aquamin) to calcium carbonate in an ovariectomised rat model of osteoporosis in order to assess Aquamin’s efficacy in preventing the onset of bone loss. Animals were randomly assigned to either non-ovariectomy control (Control), ovariectomy (OVX) plus calcium carbonate, ovariectomy plus Aquamin or ovariectomy plus Aquamin delay where Aquamin treatment started 8 weeks post OVX. At the end of …

Design Of A Compact, Fully-Autonomous 433 Mhz Tunable Antenna For Wearable Wireless Sensor Applications, John Buckley, Kevin Mccarthy, Domenico Gaetano, Loizos Loizou, Brendan O'Flynn, Cian O'Mathuna

Articles

The authors present the design of a tunable 433 MHz antenna that is tailored for wearable wireless sensor applications. This study first presents a detailed analysis of the measured impedance characteristics of a chosen antenna under test (AUT) in varying proximity to a human test subject. Instead of limiting the analysis to the head and hand only, this analysis measures the AUT impedance at varying distances from 11 different body positions. A novel antenna equivalent circuit model is then developed that enables both the free-space and total on-body AUT impedance variation to be rapidly computed using a circuit simulator instead …

Investigating Industry Expert Discourses On Aspirational Csr Communication, Lisa Koep

Articles

Purpose – The purpose of this paper is to investigate industry expert discourses on aspirational corporate social responsibility (CSR) communication. Analysing CSR managers’ and communication consultants’ talk about aspirational talk as constitutive of aspirational CSR communication, the data provide valuable insights into the dominant discourses, and draw attention to the manifold elements in the process of aspirational CSR communication. Design/methodology/approach – Data gathered during 11 in-depth, qualitative interviews with food industry experts in CSR and CSR communication roles in Ireland, the UK and the USA are studied. Findings – The analysis of industry expert discourses suggests that communicating CSR, and …

How To Use Analogies For Creative Business Modelling, Ryan Rumble, Niall Minto

Articles

Purpose

This paper aims to present a method for interpreting and reinterpreting business models as analogies to support the creation of new business model ideas.

Design/methodology/approach

The authors use the literature on cognitive frames and attention to demonstrate the often-overlooked potential of analogies. From this, the authors derive practical recommendations for the use of analogies in creative business model design.

Findings

Managers can design creative business models by seeking multiple interpretations of the way other businesses create and capture value.

Originality/value

Business model frameworks are commonplace, but there is little discussion on how to use them effectively. Furthermore, while analogies …

Health Care For All In Ireland? The Consequences Of Politics For Health Policy, Vivienne Byers

Articles

The Irish health-care system is a complicated mix of public and private providers, with inequitable and unclear routes for health-service users to access and navigate the system. In 2011, the Irish Government committed to significant health reform to develop a universal single-tier health system. In line with other European nations this was to be underpinned by the principle of social solidarity, with equitable access based on need rather than ability to pay. The road to this reform and its recent collapse highlights the practical implications of political and policy choices in health care, and has implications for financing and delivery, …

A Six Stage Operational Framework For Individualising Injury Risk Management In Sport, Mark Roe, Shane Malone, Catherine Blake, Kieran Collins, Conor Gissane, Fionn Buttner, John C. Murphy, Eamonn Delahunt

Articles

Managing injury risk is important for maximising athlete availability and performance. Although athletes are inherently predisposed to musculoskeletal injuries by participating in sports, etiology models have illustrated how susceptibility is influenced by repeat interactions between the athlete (i.e. intrinsic factors) and environmental stimuli (i.e. extrinsic factors). Such models also reveal that the likelihood of an injury emerging across time is related to the interconnectedness of multiple factors cumulating in a pattern of either positive (i.e. increased fitness) or negative adaptation (i.e. injury).

Hypoxia Reduces The Pathogenicity Of Pseudomonas Aeruginosa By Decreasing The Expression Of Multiple Virulence Factors, Bettina Schaible, Javier Rodriguez, Amaya Garcia, Alexander Von Kriegsheim, Siobhan Mcclean, Caitríona Hickey, Ciara E. Keogh, Eric Brown, Kirsten Schaffer, Alexis Broquet, Cormac T. Taylor

Articles

Our understanding of how the course of opportunistic bacterial infection is influenced by the microenvironment is limited. We demonstrate that the pathogenicity of Pseudomonas aeruginosa strains derived from acute clinical infections is higher than that of strains derived from chronic infections, where tissues are hypoxic. Exposure to hypoxia attenuated the pathogenicity of strains from acute (but not chronic) infections, implicating a role for hypoxia in regulating bacterial virulence. Mass spectrometric analysis of the secretome of P. aeruginosa derived from an acute infection revealed hypoxia-induced repression of multiple virulence factors independent of altered bacterial growth. Pseudomonas aeruginosa lacking the Pseudomonas prolyl-hydroxylase …

Macrophage Migration Inhibitory Factor Enhances Pseudomonas Aeruginosa Biofilm Formation, Potentially Contributing To Cystic Fibrosis Pathogenesis, Aisling Tynan, Leona Mawhinney, Michelle Armstrong, Ciaran O'Reilly, Sarah Kennedy

Articles

Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator that we have previously shown to be associated with an aggressive clinical phenotype in cystic fibrosis. It possesses unique tautomerase enzymatic activity. However, to date, no human-derived substrate has been identified that has the capacity to interact with this cytokine's unique tautomerase activity. This led us to hypothesize that MIF may have the capacity to interact with external substrates. We describe for the first time how Pseudomonas aeruginosa can utilize human recombinant MIF (rMIF) to significantly (P < 0.01) enhance its endogenous biofilm formation. Our in vivo studies demonstrate that utilizing a small-molecular-weight inhibitor targeting MIF's tautomerase activity (SCD-19) significantly reduces the inflammatory response in a murine pulmonary chronic P. aeruginosa model. In addition, we show that in in vitro experiments, pretreatment of P. aeruginosa with rMIF is associated with reduced bacterial killing by tobramycin. Our novel findings support the concept of an anti-MIF strategy that targets this enzymatic activity as a potential future antibacterial therapeutic approach.-Tynan, A., Mawhinney, L., Armstrong, M. E., O'Reilly, C., Kennedy, S., Caraher, E., Jülicher, K., O'Dwyer, D., Maher, L., Schaffer, K., Fabre, A., McKone, E. F., Leng, L., Bucala, R., Bernhagen, J., Cooke, G., Donnelly, S. C. Macrophage migration inhibitory factor enhances Pseudomonas aeruginosa biofilm formation, potentially contributing to cystic fibrosis pathogenesis.

Effects Of Carbohydrate Mouth Rinse And Caffeine On High-Intensity Interval Running In A Fed State, Simon Devenney, Shane Mangan, Marcus Shortall, Kieran Collins

Articles

The current study aims to identify if mouth rinsing with a 6% carbohydrate mouth-rinse (CMR) solution and mouth rinsing and ingestion of caffeine (CMR+CAFF) can affect exercise performance during steady-state (SS) running and high-intensity intervals (HIIT) in comparison with a 0% control solution (PLA) when in a fed state. Eight recreationally trained males completed 3 trials (CMR, CMR+CAFF, and PLA) of 45 min SS running and an HIIT protocol (90% peak treadmill velocity) until fatigue in a double blinded, repeated-measures study. Participants ingested a capsule of either CAFF or PLA before and after SS. Participants received a 25-mL bolus of …

Sequential Burkholderia Cenocepacia Isolates From Siblings With Cystic Fibrosis Show Increased Lung Cell Attachment, Louise Cullen, Andrew O'Connor, Pavel Drevinek, Kirsten Schaffer, Siobhan Mcclean

Articles

No abstract available

Isolation And Spectroscopic Characterization Of Zn(Ii), Cu(Ii), And Pd(Ii) Complexes Of 1,3,4-Thiadiazole-Derived Ligand, Dariusz Karcz, Arkadiusz Matwijczuk, Bozena Boron, Bernadette Creaven, Leszek Fiedor, Andrzej Niewiadomy, Mariusz Gagos

Articles

A series of complexes incorporating Zn(II), Cu(II), and Pd(II) ions, and 4-(5-heptyl-1,3,4-thiadiazol-2-yl)benzene-1,3-diol (L1) as model ligand, was synthesized in order to examine the nature of potential interactions between biologically active ligands, 1,3,4-thiadiazoles and metal ions with proven biological relevance. The structures of the compounds isolated were characterized using a number of spectroscopic methods including IR, Uv–vis, AAS, steady state and time-resolved fluorescence (TRF). The results obtained suggest that the L1-Zn(II) and L1-Pd(II) complexes consist of one molecule of L1 and one acetate ion acting as ligands, while the L1-Cu(II) complex adapts a 2:1 (L1: metal) stoichiometry. The coordination of L1 …

Effect Of Game Design, Goal Type, And Player Numbers On The Physiological And Physical Demands Of Hurling-Specific Small-Sided Games, Shane Malone, Kieran Collins

Articles

The current study examined the effect that game design modification, goal type, and player numbers on the running performance and physiological demands of small-sided hurling games (SSG). Forty-eight hurling players (age, 25.5 ± 3.2 years; height, 178.9 ± 3.2 cm; body mass, 78.5 ± 4.5 kg) performed 4 types of SSG (possession [P], normal play [NP], regular goals [RG] and small goals [SG]) in 4-a-side, 5-a-side, and 6-a-side formats. Heart rate (Polar Electro Oy) and global positioning system technology (VX Sport, 4-Hz, Lower Hutt) were used to analyze the physical and physiological differences between SSG. Total distance (m), high-speed running …

Are Small-Sided Games An Effective Training Methodology For Improving Fitness In Hurling Players? A Comparative Study Of Training Methodologies, Shane Malone, Brian Hughes, Kieran Collins

Articles

The current investigation compared the effects of specific small-sided games training versus generic continuous aerobic training on team sport related exercise performances. Forty-eight hurling players (age: 25 ± 6.4 years; height: 180.2 ± 20.4 cm; mass: 80.5 ± 3.2 kg; V·O2max" role="presentation" style="display: inline; line-height: normal; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; padding: 0px; margin: 0px; position: relative;">V⋅O2maxV·O2max: 58.78 ± 3.05 ml·kg·min⁻¹) completed either traditional continuous aerobic training (n = 24) or team sport-specific small-sided game training (n = 24), consisting …

Monitoring Player Fitness, Fatigue Status And Running Performance During An In-Season Training Camp In Elite Gaelic Football, Shane Malone, Brian Hughes, Mark Roe, Kieran Collins, Martin Buchheit

Articles

Objectives: To examine selected perceptual and physiological measures to monitor fitness, fatigue and running performance during an in-season training camp in elite Gaelic football. Methods: Twenty-two elite Gaelic football players were monitored for training load, perceived ratings of wellness, heart rate variability (HRV; LnSD1), heart rate recovery (HRR), exercise heart rate (HRex), lower limb muscular power (CMJ) and global positioning system (GPS) variables. The Yo-Yo intermittent recovery test level 1 (Yo-YoIR1) was assessed pre and post the training camp. With specific small-sided games (SSG) used as a measure of running performance. Results: During the camp, HRex decreased (−12.2%), HRR increased …

The Running Performance Profile Of Elite Gaelic Football Match-Play, Shane Malone, Barry Solan, Kieran Collins

Articles

The current study examined (a) the match running performance of Gaelic football and (b) the decrement in match running performance with respect to position. Global positioning satellite system technologies (4-Hz; VX Sport) were used with 3 elite intercounty teams across 3 full seasons with 250 full game data sets collected. Game movements were classified according to game actions and distance covered across speed zone thresholds (total distance [TD], high-speed running distance [HSRD; ≥17 km·h], sprint distance [SD; ≥22 km·h]; accelerations [n]; peak speed [km·h]). The influence of running performance in each quarter on the subsequent quarter was analyzed across all …

The Relationship Between Technical Performance Indicators And Running Performance In Elite Gaelic Football, Shane Mangan, Martin Ryan, Simon Devenney, Aidan Shovlin, Jason Mcgahan, Shane Malone, Cian O'Neill, Con Burns, Kieran Collins

Articles

The aim of the current study was to assess whether technical performance relates to positional running demands of elite Gaelic football players. Over a period of three years (2014–2016), four elite Gaelic football teams were monitored using GPS technologies. Four hundred and thirty-two individual player samples were collected from 52 competitive matches. Video analysis software was used to code technical performance indicators in all games. Pearson’s correlations were performed between all technical variables and total distance (m) and high-speed distance (≥17 km h−1). Total ball in play time had small to medium effects (r = .233 to .390, p …

A Contemporary Multi-Modal Mechanical Approach To Training Monitoring In Elite Professional Soccer, Adam Owen, Leo Djaoui, Matthew Newton, Shane Malone, Bruno Mendes

Articles

Objectives: Understanding movements and mechanical demands of elite soccer players during training and competitive stressors is important for the support provision of player performance. Continued appreciation to quantify and monitor training load (TL) is apparent, however reporting multi-modal approaches in-line with competitive match-play demands remain limited. The investigation aimed to highlight a multimodal training monitoring method and its relationship to match-play. Subjects: 29 elite European soccer players participated were assessed (26.7 ± 4.07 years, height 183.4 ± 5.87 cm, body mass 78.4 ± 8.03 kg, 57.55 ± 5.32 ml.kg⁻¹.min⁻¹ and body composition 54.12 ± 13.65 mm) with daily …

The Patient Survey Programme: Transforming The Patient Experience In Irish Healthcare, Vivienne Byers, D. Fahey, C. Mullins, C. Roe

Articles

For more than a decade, health policy in Ireland has pushed the centrality of the patient in the delivery and management of healthcare services, including the development of a National Strategy for Service User Involvement in the Health Service 2008–2013 (Health Service Executive, 2008). More recently, the need to provide a ‘patientcentred’ (Department of Health, 2012, 2013, 2014) or ‘person-centred’ (Department of Health, 2016) service has been a guiding principle of health policy documents. The National Service Plan 2017 (Health Service Executive, 2016) also outlines the importance of increasing the rates of service user engagement and feedback across the health …

Aphonic: Adaptive Thresholding For Noise Cancellation In Smart Mobile Environments, Ruairí De Fréin

Articles

We propose a signal-channel, adaptive threshold selection technique for binary mask construction, namely APHONIC, (AdaPtive tHreshOlding for NoIse Cancellation) for smart mobile environments. Using this mask, we introduce two noise cancellation techniques that perform robustly in the presence of real-world interfering signals that are typically encountered by mobile users: a violin busker, a subway and busy city square sounds. We demonstrate that when the power of the time-frequency components of the voice of a mobile user does not significantly overlap with the components of the interference signal, the threshold learning and noise cancellation techniques significantly improve the Signal-to-Interference Ratio (SIR) …

Subjective Usability, Mental Workload Assessments And Their Impact On Objective Human Performance, Luca Longo

Articles

Self-reporting procedures and inspection methods have been largely employed in the fields of interaction and web-design for assessing the usability of interfaces. However, there seems to be a propensity to ignore features related to end-users or the context of application during the usability assessment procedure. This research proposes the adoption of the construct of mental workload as an additional aid to inform interaction and web-design. A user-study has been performed in the context of human-web interaction. The main objective was to explore the relationship between the perception of usability of the interfaces of three popular web-sites and the mental workload …

Longer-Term Impact Of Highand Low Temperature On Mortality: An International Study To Clarify Length Of Mortality Displacement, Ben Armstrong, Michelle Bell, Micheline De Sousa Zanotti Stagliorio Coelho, Yue Liang Leon Guo, Yuming Guo, Patrick J. Goodman, Masahiro Hashizume, Yasushi Honda, Ho Kim, Eric Lavigne, Paola Michelozzi, Paulo Hilario Nascimento Saldiva, Joel Schwartz, Matteo Scortichini, Francesco Sera, Aurelio Tobias, Shilu Tong, Chang Fu Wu, Antonella Zanobetti, Ariana Zeka, Antonio Gasparrini

Articles

In many places, daily mortality has been shown to increase after days with particularly high or low temperatures, but such daily time-series studies cannot identify whether such increases reflect substantial life shortening or short-term displacement of deaths (harvesting).

Vitamin D Improves Selected Metabolic Parameters But Not Neuropsychological Or Quality Of Life Indices In Osa: A Pilot Study, Conor Kerley, Katrina Hutchinson, Jessica Branham, Aisling Mcgowan, John Faul, Liam Cormican

Articles

Study Objectives: Our group and others have reported a high rate of vitamin D deficiency in obstructive sleep apnea (OSA), where vitamin D levels (25(OH) D) correlate negatively with OSA severity and some of its associated metabolic alterations. Data regarding vitamin D supplementation in OSA are lacking. We wanted to evaluate the effect of vitamin D3 supplementation on OSA symptoms and metabolic parameters. Methods: We conducted a pilot, double-blind, randomized, placebo-controlled trial of daily supplementation with 4,000 IU vitamin D3 (D3) or placebo (PL). We studied 19 Caucasian adults (14 male, mean age 55 y, mean body mass index [BMI] …