Digital Commons Network^™

The Statistical Optimisation Of Recombinant Β-Glucosidase Production Through A Two-Stage, Multi-Model, Design Of Experiments Approach, Albert Uhoraningoga, Gemma K. Kinsella, Jesus Maria Frias, Gary T. Henehan, Barry J. Ryan

Articles

β-glucosidases are a class of enzyme that are widely distributed in the living world, with examples noted in plants, fungi, animals and bacteria. They offer both hydrolysis and synthesis capacity for a wide range of biotechnological processes. However, the availability of native, or the production of recombinant β-glucosidases, is currently a bottleneck in the widespread industrial application of this enzyme. In this present work, the production of recombinant β-glucosidase from Streptomyces griseus was optimised using a Design of Experiments strategy, comprising a two-stage, multi-model design. Three screening models were comparatively employed: Fractional Factorial, Plackett-Burman and Definitive Screening Design. Four variables …

Dignity And Civility, Reconsidered, Leah Litman

Articles

People often talk about the Chief Justice, Justice Kagan, and Justice Breyer as the institutionalists on the modern Supreme Court. And that’s true, they are. Those Justices care about the Court as an institution and the Court’s reputation. They do not want people to look at the Court as a set of politicians in robes; and they do not want people to see judges as having ideological or partisan agendas. That is how they think of themselves, and they are willing to make compromises to maintain that image of the Court, and to set aside their personal beliefs in order …

Grants, Nicholson Price Ii

Articles

Innovation is a primary source of economic growth and is accordingly the target of substantial academic and government attention. Grants are a key tool in the government’s arsenal to promote innovation, but legal academic studies of that arsenal have given them short shrift. Although patents, prizes, and regulator-enforced exclusivity are each the subject of substantial literature, grants are typically addressed briefly, if at all. According to the conventional story, grants may be the only feasible tool to drive basic research, as opposed to applied research, but they are a blunt tool for that task. Three critiques of grants underlie this …

Scrutinizing Anticompetitive State Regulations Through Constitutional And Antitrust Lenses, Daniel A. Crane

Articles

State and local regulations that anticompetitively favor certain producers to the detriment of consumers are a pervasive problem in our economy. Their existence is explicable by a variety of structural features—including asymmetry between consumer and producer interests, cost externalization, and institutional and political factors entrenching incumbent technologies. Formulating legal tools to combat such economic parochialism is challenging in the post-Lochner world, where any move toward heightened judicial review of economic regulation poses the perceived threat of a return to economic substantive due process. This Article considers and compares two potential tools for reviewing such regulations—a constitutional principle against anticompetitive parochialism …

Mindfulness, Mental Health, And Wellness, Scott L. Rogers

Articles

No abstract provided.

Should Automakers Be Responsible For Accidents?, Kyle D. Logue

Articles

Motor vehicles are among the most dangerous products sold anywhere. Automobiles pose a larger risk of accidental death than any other product, except perhaps opioids. Annual autocrash deaths in the United States have not been below 30,000 since the 1940s, reaching a recent peak of roughly 40,000 in 2016. And the social cost of auto crashes goes beyond deaths. Auto-accident victims who survive often incur extraordinary medical expenses. Those crash victims whose injuries render them unable to work experience lost income. Auto accidents also cause nontrivial amounts of property damage—mostly to the automobiles themselves, but also to highways, bridges, or …

The Science Of Mindfulness And The Practice Of Law, Scott L. Rogers, Amishi P. Jha

Articles

No abstract provided.

Neglecting Nationalism, Gil Seinfeld

Articles

Federalism is a system of government that calls for the division of power between a central authority and member states. It is designed to secure benefits that flow from centralization and from devolution, as well as benefits that accrue from a simultaneous commitment to both. A student of modern American federalism, however, might have a very different impression, for significant swaths of the case law and scholarly commentary on the subject neglect the centralizing, nationalist side of the federal balance. This claim may come as a surprise, since it is obviously the case that our national government has become immensely …

Pka, Caspase 1 And Hsp40 Induced Apoptosis Under Fungi Starvation, Tobiloba Sojinrin, Kangze Liu, Daxiang Cui, James Curtin, Furong Tian

Articles

To investigate the influence of starvation on the biochemical response of Aspergillus niger. The biochemical impact of starvation was determined by morphological observation, immunofluorescent analysis, High-performance liquid chromatography (HPLC) and western blot over 8 days. Results showed that starvation can inhibit fungi survival rate in a time-dependent manner. A. niger exhibited active responses to starvation such as secretion of some 40 kDa proteins to manage changes in water balance. Conidiophores disintegrated from lack of nutrient. The immunofluorescent analysis demonstrated elevated ROS accumulation in starved cells (PA. niger growth by inducing cell apoptosis.

Whistleblowing In The Irish Military: The Cost Of Exposing Bullying And Sexual Harassment, John Hogan, Sharon Feeney, Grace Flynn

Articles

Whistleblowing has gained increasing media attention over the past 40 years, as incidents of abuse and wrongdoing associated with businesses, religious institutions, the media and politics have come to light. In this article, we investigate the consequences of a military whistleblower’s actions for both himself and the military institution that he was a part of. The case concerns former army officer Dr. Tom Clonan and his findings concerning the bullying and sexual harassment of female personnel in the Irish Defence Forces at the turn of the century. As these revelations came to light over 17 years ago we are able …

Analysis Of Human Mitochondrial Dna Content By Southern Blotting And Nonradioactive Probe Hybridization., Joel H Wheeler, Carolyn K J Young, Matthew J Young

Articles

A single cell can contain several thousand copies of the mitochondrial DNA genome or mtDNA. Tools for assessing mtDNA content are necessary for clinical and toxicological research, as mtDNA depletion is linked to genetic disease and drug toxicity. For instance, mtDNA depletion syndromes are typically fatal childhood disorders that are characterized by severe declines in mtDNA content in affected tissues. Mitochondrial toxicity and mtDNA depletion have also been reported in human immunodeficiency virus-infected patients treated with certain nucleoside reverse transcriptase inhibitors. Further, cell culture studies have demonstrated that exposure to oxidative stress stimulates mtDNA degradation. Here we outline a Southern …

Interactions Between Diet, Physical Activity, And The Sociocultural Environment For Older Adult Health In The Urban Subarctic., Britteny M. Howell

Articles

No abstract provided.

Reputation As A Disciplinarian Of International Organizations, Kristina Daugirdas

Articles

As a disciplinarian of international organizations, reputation has serious shortcomings. Even though international organizations have strong incentives to maintain a good reputation, reputational concerns will sometimes fail to spur preventive or corrective action. Organizations have multiple audiences, so efforts to preserve a “good” reputation may pull organizations in many different directions, and steps taken to preserve a good reputation will not always be salutary. Recent incidents of sexual violence by UN peacekeepers in the Central African Republic illustrate these points.

Gne: A Deep Learning Framework For Gene Network Inference By Aggregating Biological Information, Kishan Kc, Rui Li, Feng Cui, Qi Yu, Anne R. Haake

Articles

BACKGROUND: The topological landscape of gene interaction networks provides a rich source of information for inferring functional patterns of genes or proteins. However, it is still a challenging task to aggregate heterogeneous biological information such as gene expression and gene interactions to achieve more accurate inference for prediction and discovery of new gene interactions. In particular, how to generate a unified vector representation to integrate diverse input data is a key challenge addressed here. RESULTS: We propose a scalable and robust deep learning framework to learn embedded representations to unify known gene interactions and gene expression for gene interaction predictions. …

Deploying Mindfulness To Gain Cognitive Advantage: Considerations For Military Effectiveness And Well-Being, Amishi P. Jha, Scott L. Rogers, Eric Schoomaker, Edward Cardon

Articles

Mindfulness involves paying attention to present moment experience without discursive commentary or emotional reactivity. Mindfulness training (MT) programs aim to promote this mental mode via introduction to specific mindfulness exercises, related in-class discussion, and ongoing engagement in mindfulness exercises. MT is being increasingly offered to high-demand, high-stress military/uniformed and civilian cohorts with a wide array of reported benefits. Herein, we begin by discussing recent theoretical models regarding MT’s mechanisms of action from a cognitive training/cognitive neuroscience perspective, which propose that MT engages and strengthens three key processes [e.g., 1]. These are: 1) attentional orienting, which is the ability to select …

Barriers To The Advancement Of Women Of Color Faculty In Stem: The Need For Promoting Equity Using An Intersectional Framework, Maya Corneille, Anna Lee, Sherrice Allen, Jessica Cannady, Alexia Guess

Articles

Purpose–The purpose of this paper is to highlight critical issues facing women of color (WOC) faculty and to synthesize the research literature in order to offer recommendations for action to address inequities using an intersectionality framework.

Design/methodology/approach–The authors conducted a qualitative meta-analysis. Relevant articles were obtained through a search of the EBSCO and Google Scholar databases entering in combinations of specific keywords. In order to be included in this review, the manuscripts had to be published between the years 2001 and 2017; in a peer-reviewed journal; and available through the university library system.

Findings–The majority of manuscripts …

Disparate Impact, Unified Law, Nicholas Stephanopoulos

Articles

No abstract provided.

Patents, Property, And Prospectivity, Jonathan Masur, Adam Mortara

Articles

No abstract provided.

Assessing The Empirical Upside Of Personalized Criminal Procedure, Matthew B. Kugler, Lior Strahilevitz

Articles

No abstract provided.

Norming In Administrative Law, Jonathan Masur, Eric A. Posner

Articles

No abstract provided.

Not Such A Fixed Star After All: West Virginia State Board Of Education V. Barnette, And The Changing Meaning Of The First Amendment Right Not To Speak, Genevieve Lakier

Articles

No abstract provided.

Pharmaceutical Industry Support Of U.S. Patient Advocacy Organizations: An International Context, So Yeon Kang, Ge Bai, Laura Karas, Gerard F. Anderson

Articles

No abstract provided.

An Investigation Of The Detection Capability Of Pulsed Wave Duplex Doppler Of Low Grade Stenosis Using Ultrasound Contrast Agent Microbubbles – An In-Vitro Study, Jacinta Browne, Deirdre King, Andrew J. Fagan, Deepa Chari, Carmel Moran

Articles

Objective: The objective of the study was to investigate whether clinically used ultrasonic contrast agents improved the accuracy of spectral Doppler ultrasound in the detection of low grade (< 50%) renal artery stenosis. Low grade stenoses in the renal artery are notoriously difficult to reliably detect using Doppler ultrasound due to difficulties such as overlying fat and bowel gas.

Methods: A range of anatomically-realistic renal artery phantoms with varying low degrees of stenosis (0, 30 and 50%) were constructed and peak velocity data was measured from within the pre-stenotic and mid-stenotic regions in each phantom, for both unenhanced and contrast-enhanced spectral Doppler data acquisitions. The effect of a 20mm overlying fat layer on the ultrasound beam distortion and phase aberration, and hence on the measured peak velocity data, was also …

Tribes, Cities, And Children: Emerging Voices In Environmental Litigation, Nina A. Mendelson

Articles

an environmental nongovernmental organization ("NGO") on behalf of a neighbor or hiker.1 The NGO would allege that the individual faced health risks, that her property was contaminated, or that she could no longer hike, fish, swim, or view wildlife such as the endangered Nile crocodile, as in the well-known case of Lujan v. Defenders of Wildlife.

International Law And Political Philosophy: Uncovering New Linkages, Steven R. Ratner

Articles

The legal regime regulating cross-border investment gives key rights to foreign investors and places significant duties on states hosting that investment. It also raises distinctive moral questions due to its potential to constrain a state’s ability to manage its economy and protect its people. Yet international investment law remains virtually untouched as a subject of philosophical inquiry. The questions of international political morality surrounding investment rules can be mapped through the lens of two critiques of the law – that it systemically takes advantage of the global South and that it constrains the policy choices of states hosting investment. Each …

Monitoring Meaningful Activities Using Small Low-Cost Devices In A Smart Home, Jordan Tewell, Dympna O'Sullivan, Neil Maiden, James Lockerbie, Simone Stumpf

Articles

A challenge associated with an ageing population is increased demand on health and social care, creating a greater need to enable persons to live independently in their own homes. Ambient assistant living technology aims to address this by monitoring occupants’ ‘activities of daily living’ using smart home sensors to alert caregivers to abnormalities in routine tasks and deteriorations in a person’s ability to care for themselves. However, there has been less focus on using sensing technology to monitor a broader scope of so-called ‘meaningful activities’, which promote a person’s emotional, creative, intellectual, and spiritual needs. In this paper, we describe …

The Security Field: Forming And Expanding A Bourdieusian Criminology, Matt Bowden

Articles

Recent scholarly contributions have sought to integrate Bourdieusian sociology with criminology, centring for example, on the ‘street’ field as a symbolic and narrative space occupied by players within criminal justice. This article complements this broad objective by focusing on the changes in contemporary police and security governance that are pointing towards an emerging security field. Such a change can be read from the literature on plural policing and crime control, and involves the morphology of policing into nodes or assemblages of security producers. While there has been some attention to the formation of security networks, further empirical work is required …

Ly379268 Does Not Have Long-Term Procognitive Effects Nor Attenuate Glutamatergic Signaling In Aβpp/Ps1 Mice., Kevin N Hascup, Jesse Britz, Caleigh Findley, Shelley Tischkau, Erin R. Hascup

Articles

No abstract provided.

The Americans With Disabilities Act: Legal And Practical Applications In Child Protection Proceedings, Joshua B. Kay

Articles

Parents with disabilities, particularly those with intellectual disability and/or mental illness, are disproportionately represented in the child protection system.1 Once involved in the system, they are far more likely than parents without disabilities to have their children removed and their parental rights terminated. The reasons for this are many. Parents with disabilities are relatively likely to experience other challenges that are themselves risk factors for child protection involvement. In addition, child protection agencies, attorneys, courts, and related professionals often lack knowledge and harbor biases about parents with disabilities, increasing the likelihood of more intrusive involvement in the family. Yet research …

Effect Of Fermentation On Enhancing The Nutraceutical Properties Of Arthrospira Platensis (Spirulina), Elena Demarcocastro, Emer Shannon, Nissreen Abu-Ghannam

Articles

Abstract:Arthrospira platensis(spirulina), a filamentous fresh-water planktonic cyanobacterium,possesses diverse biological activities and a unique nutritional profile, due to its high contentof valuable nutrients. This study aimed to further improve the bioactive profile of spirulina, byfermenting it with the lactic acid bacteriumLactobacillus plantarum.In vitrocomparison of thetotal phenolic content (TPC), C-phycocyanin, free methionine, DPPH radical scavenging capacity,ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC) and proteinfragmentation via SDS-PAGE in untreated versus 12 to 72 h fermented spirulina is reported here.After 36 h fermentation, TPC was enhanced by 112%, FRAP by 85% and ORAC by 36%. After 24 h,the DPPH radical scavenging …