Digital Commons Network^™

Genetic Background Of Antimicrobial Resistance In Multiantimicrobial-Resistant Escherichia Coli Isolates From Feces Of Healthy Broiler Chickens In Tunisia, Mohamed Salah Abbassi, Hajer Kilani, Islem Abid, Yolanda Sáenz, Paul Hynds, Sana Lengliz, Noureddine Ben Chehida, Ilhem Boutiba-Ben Boubaker

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Multiantimicrobial-resistant Escherichia coli isolates are a global human health problem causing increasing morbidity and mortality. Genes encoding antimicrobial resistance are mainly harbored on mobile genetic elements (MGEs) such as transposons and plasmids as well as integrons, which enhance their rapid spread. The aim of this study was to characterize 83 multiantimicrobial-resistant E. coli isolates recovered from healthy broiler chickens. Among 78 tetracycline-resistant isolates, the tetA, tetB, and tetC genes were detected in 59 (75.6%), 14 (17.9%), and one (1.2%) isolates, respectively. The sul1, sul2, and sul3 genes were detected 31 (46.2%), 16 (23.8%), and 6 (8.9%) isolates, respectively, among 67 …

Spatiotemporal Dynamics Of Sporadic Shiga Toxin–Producing Escherichia Coli Enteritis, Ireland, 2013–2017, Eiimear Cleary, Patricia Garvey, Paul Mckeown, Jean O'Dwyer, Coilin Oh Aiseadha, Paul Mckeown, Paul Hynds

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The Republic of Ireland regularly reports the highest annual crude incidence rates of Shiga toxin–producing Escherichia coli (STEC) enteritis in the European Union, ≈10 times the average. We investigated spatiotemporal patterns of STEC enteritis in Ireland using multiple statistical tools. Overall, we georeferenced 2,755 cases of infection during January 2013–December 2017; we found >1 case notified in 2,340 (12.6%) of 18,641 Census Small Areas. We encountered the highest case numbers in children 0–5 years of age (n = 1,101, 39.6%) and associated with serogroups O26 (n = 800, 29%) and O157 (n = 638, 23.2%). Overall, we identified 17 space-time …

Spatiotemporal Epidemiology Of Cryptosporidiosis In The Republic Of Ireland, 2008–2017: Development Of A Space–Time “Cluster Recurrence” Index, Martin Boudou

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Approximately 1.5 million individuals in Ontario are supplied by private water wells (private groundwater supplies). Unlike municipal supplies, private well water quality remains unregulated, with owners responsible for testing, treating, and maintaining their own water supplies. The COVID-19 global pandemic and associated non-pharmaceutical interventions (NPIs) have impacted many environmental (e.g., surface water and air quality) and human (e.g., healthcare, transportation) systems over the past 15-months (January 2020 to March 2021). To date, the impact of these interventions on private groundwater systems remains largely unknown. Accordingly, the current study aimed to investigate the impact of a province-wide COVID-19 lockdown (late-March 2020) …

Mapping The Light Fantastic At Newgrange, Frank Prendergast, Clare Tuffy, John Lalor, Claire Breen, Sinéad Gargan

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THE WORLD HERITAGE PROPERTY of Brú na Bóinne attracts thousands of visitors from Ireland and around the globe, many drawn by the remarkable winter solstice phenomenon, when the rising sun’s rays illuminate the burial chamber. During 2020 it became clear that public health measures to combat the global pandemic were going to preclude visitor access to the chamber of the Great Mound of Newgrange, including during the annual winter solstice celebrations. When the government agencies OPW and NMS discussed how to manage Newgrange and the solstice during the restrictions, Clare Tuffy, Manager of Visitor Services at Brú na Bóinne, suggested …

Mammalian Nuclear Trub1, Mitochondrial Trub2, And Cytoplasmic Pus10 Produce Conserved Pseudouridine 55 In Different Sets Of Trna., Shaoni Mukhopadhyay, Manisha Deogharia, Ramesh Gupta

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Most mammalian cytoplasmic tRNAs contain ribothymidine (T) and pseudouridine (Ψ) at positions 54 and 55, respectively. However, some tRNAs contain Ψ at both positions. Several Ψ54-containing tRNAs function as primers in retroviral DNA synthesis. The Ψ54 of these tRNAs is produced by PUS10, which can also synthesize Ψ55. Two other enzymes, TRUB1 and TRUB2, can also produce Ψ55. By nearest-neighbor analyses of tRNAs treated with recombinant proteins and subcellular extracts of wild-type and specific Ψ55 synthase knockdown cells, we determined that while TRUB1, PUS10, and TRUB2 all have tRNA Ψ55 synthase activities, they have different tRNA structural requirements. Moreover, these …

Defining Who Is An Employee After A.B.5: Trading Uniformity And Simplicity For Expanded Coverage, Edward A. Zelinsky

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My assessment of California’s A.B.5 differs from the evaluation advanced by the advocates and opponents of that legislation: I conclude that A.B.5 made a significant but limited expansion of the coverage of California labor law but at a notable cost. Even as A.B.5 broadened the reach of the Golden State’s labor protections, A.B.5 also made the definition of "employee” more complex and less uniform. Those seeking federal or state legislation like A.B.5 confront the same trade-off under which greater coverage is achieved at the expense of more complexity and less uniformity in the definition of who is an employee. The …

The Rise Of Decentralized Autonomous Organizations: Opportunities And Challenges, Aaron J. Wright

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The article explores the rise of Decentralized Autonomous Organizations (DAOs), highlighting their potential to transform traditional organizational structures through blockchain technology and smart contracts. DAOs offer operational efficiencies, such as decentralized governance and transparent decision-making, but face significant legal challenges, including lack of recognition, liability concerns, and regulatory uncertainties. The article argues that U.S. business law, with its emphasis on private ordering, could accommodate DAOs, but legal adaptations are necessary to address their unique characteristics and ensure widespread adoption.

Entitlement To Punishment, Kyron J. Huigens

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This Article advances the idea of entitlement to punishment as the core of a normative theory of legal punishment's moral justification. It presents an alternative to normative theories of punishment premised on desert or public welfare; that is, to retributivism and consequentialism. The argument relies on H.L.A. Hart's theory of criminal law as a "choosing system," his theory of legal rules, and his theory of rights. It posits the advancement of positive freedom as a morally justifying function of legal punishment.

An entitlement to punishment is a unique, distinctive legal relation. We impose punishment when an offender initiates an ordered …

Police Prosecutions And Punitive Instincts, Kate Levine

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This Article makes two contributions to the fields of policing and criminal legal scholarship. First, it sounds a cautionary note about the use of individual prosecutions to remedy police brutality. It argues that the calls for ways to ease the path to more police prosecutions from legal scholars, reformers, and advocates who, at the same time, advocate for a dramatic reduction of the criminal legal system’s footprint, are deeply problematic. It shows that police prosecutions legitimize the criminal legal system while at the same time displaying the same racism and ineffectiveness that have been shown to pervade our prison-backed criminal …

Distinct Chemistries Define The Diverse Biological Effects Of Plasma Activated Water Generated With Spark And Glow Plasma Discharges, Evanthia Tsoukou, Maxime Delit, Louise Treint, Paula Bourke, Daniela Boehm

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The spread of multidrug-resistant bacteria poses a significant threat to human health. Plasma activated liquids (PAL) could be a promising alternative for microbial decontamination, where different PAL can possess diverse antimicrobial efficacies and cytotoxic profiles, depending on the range and concentration of their reactive chemical species. In this research, the biological activity of plasma activated water (PAW) on different biological targets including both microbiological and mammalian cells was investigated in vitro. The aim was to further an understanding of the specific role of distinct plasma reactive species, which is required to tailor plasma activated liquids for use in applications where …

Influence Of Molecular Weight Fractionation On The Antimicrobial And Anticancer Properties Of A Fucoidan Rich-Extract From The Macroalgae Fucus Vesiculosus, Eduarda M. Cabral, Julie Mondala, Márcia Oliveira, Joanna Przyborska, Stephen Fitzpatrick, Dilip K. Rai, Saravana Periaswamy Sivagnanam, Marco Garcia-Vaquero, Denis O'Shea, Michael Devereux, Brijesh K. Tiwari, James Curtin

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The objective of this study was to investigate the antimicrobial and anticancer properties of a fucoidan extract and subsequent fractions isolated from the macroalgae Fucus vesiculosus. The fractions obtained (>300 kDa,kDa,kDa,kDa) could inhibit the growth of B. subtilis, E. coli, L. innocua and P. fluorescens when assayed at concentrations between 12,500 and 25,000 ppm. The bacterial growth was monitored by optical density (OD) measurements (600 nm, 24 h) at 30 °C or 37 °C, depending upon on the strain used. The extracted fractions were also tested for cytotoxicity against brain glioblastoma cancer cells using the Alamar Blue assay for …

Assessing A National Nanotechnology Infrastructure For Enforcing Nanosafety In Consumer Food - Funding The Infrastructure, Gordon Chambers, E. Mccarron

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. Crucial to assessing any national risk assessment infrastructure is the development of keen insights into the funding landscape, the base skill set and expertise levels, risk prioritisation, and stakeholder determination. This paper presents an overview of the first of these criteria with respect to the Irish funding landscape for nanotechnology and nano-agri-food. It examines difficulties with policy enforcement due to a lack of clarity and varying interpretations of the EU definition of nanomaterials and how the funding landscape could potentially facilitate the necessary infrastructure, to underpin regulatory enforcement and risk assessment for nanotechnology in food. In 2008 an assessment …

Rees/Aaee Special Focus On Ethics: Introduction By Guest Editor, Shannon Chance, Shannon Chance

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Today, more than ever, we see the significance of ethics in developing and sustaining the built, social, and natural environments around us. We also recognise the need to develop engineering students’ knowledge, skills, and values regarding engineering, innovation, design, production, and the like. Engineers’ actions can have intended as well as unintended consequences, and we as a community need to become more aware of the outcomes and implications of our work. In response to the many topics presented at the REES 2019, the REEN Board selected ethics as the focus for this special issue and joined with AAEE to publish …

A Call For An Intersectional Feminist Restorative Justice Approach To Addressing The Criminalization Of Black Girls, Donna Coker, Thalia Gonzalez

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No abstract provided.

Effect Of Mutation And Vaccination On Spread, Severity, And Mortality Of Covid-19 Disease, Dr Hossam Zawbaa, Hasnaa Osama, Ahmed El‐Gendy, Haitham Saeed, Hadeer S. Harb, Yasmin M. Madney, Mona Abdelrahman, Marwa Mohsen, Ahmed M.A. Ali, Mina Nicola, Marwa O. Elgendy, Ihab A. Ibrahim, Mohamed E.A. Abdelrahim

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Coronavirus disease 2019 (COVID-19) has had different waves within the same country. The spread rate and severity showed different properties within the COVID-19 different waves. The present work aims to compare the spread and the severity of the different waves using the available data of confirmed COVID-19 cases and death cases. Real-data sets collected from the Johns Hopkins University Center for Systems Science were used to perform a comparative study between COVID-19 different waves in 12 countries with the highest total performed tests for severe acute respiratory syndrome coronavirus 2 detection in the world (Italy, Brazil, Japan, Germany, Spain, India, …

Socio-Economic Factors Associated With The Incidence Of Shiga-Toxin Producing Escherichia Coli (Stec) Enteritis And Cryptosporidiosis In The Republic Of Ireland, 2008–2017, Eiimear Cleary, Martin Boudou, Coilín Oscar Óhaiseadha, Patricia Garvey, Paul Mckeown, Jean O'Dwyer, Paul Hynds

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The Republic of Ireland (ROI) currently reports the highest incidence rates of Shiga-toxin producing Escherichia coli (STEC) enteritis and cryptosporidiosis in Europe, with the spatial distribution of both infections exhibiting a clear urban/rural divide. To date, no investigation of the role of socio-demographic profile on the incidence of either infection in the ROI has been undertaken. The current study employed bivariate analyses and Random Forest classification to identify associations between individual components of a national deprivation index and spatially aggregated cases of STEC enteritis and cryptosporidiosis. Classification accuracies ranged from 78.2% (STEC, urban) to 90.6% (cryptosporidiosis, rural). STEC incidence was …

Climate-Induced Human Displacement And Conservation Lands, Jessica Owley

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As climate change leads to both internal displacement and mass migrations, we need not only new places for people to live but also new locations for infrastructure projects and other public needs. Some of the most attractive areas for these new land uses are currently unoccupied land, including land set aside for conservation. Numerous laws restrict the availability and possible uses of public conservation land. Individual agreements and property restrictions encumber private conservation land, varying in the ease with which the restrictions can be modified. For example, privately protected areas in the United States are often encumbered with perpetual conservation …

Government Speech And First Amendment Capture, Caroline Mala Corbin

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Alarm regarding government speech is not new. In earlier decades, scholars worried that the government's speech might monopolize a marketplace and drown out opposing viewpoints. But today, using a move I term "First Amendment capture," the government need not be the loudest speaker because it can become the only speaker. First Amendment capture has been made possible by the Supreme Court's developing government speech doctrine, which holds that government speech is not subject to the Free Speech Clause. Consequently, once speech is declared governmental, the government may censor viewpoints it does not like. First Amendment capture categorizing contested speech as …

Pandemics And International Law: The Need To Strengthen International Legal Frameworks After The Covid-19 Global Health Pandemic [Sierra Leone], Charles C. Jalloh

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No abstract provided.

The Least Of These: The Case For Nationwide Injunctions In Immigration Cases As A Critical Democratic Institution, Allen Slater, Richard Delgado

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No abstract provided.

Unintended Legislative Inertia, Mirit Eyal-Cohen

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Institutional and political forces create strong inertial pressures that make updating legislation a difficult task. As a result, laws often stagnate, leading to the continued existence of obsolete rules and policies that serve long-forgotten purposes. Recognizing this inertial power, legislatures over the last few decades have increasingly relied on a perceived solution -- temporary legislation. In theory, this measure avoids inertia by requiring legislators to choose to extend a law deliberately.

This Article argues that temporary legislation is a double-edged sword. While some temporary laws ultimately expire, many perpetuate through cycles of extension and reauthorization. Temporary legislation often creates its …

Covid-19 Relief And The Ordinary Inmate Symposium, Jenny E. Carroll

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No abstract provided.

Geocoding Cryptosporidiosis Cases In Ireland (2008–2017)—Development Of A Reliable, Reproducible, Multiphase Geocoding Methodology, Lisa Domegan, Patricia Garvey, Paul Mckeown, Howard Johnson, Paul Hynds, Jean O'Dwyer, Coilín Óhaiseadha

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Background: Geocoding (the process of converting a text address into spatial data) quality may affect geospatial epidemiological study findings. No national standards for best geocoding practice exist in Ireland. Irish postcodes (Eircodes) are not routinely recorded for infectious disease notifications and > 35% of dwellings have non-unique addresses. This may result in incomplete geocoding and introduce systematic errors into studies.

Aims: This study aimed to develop a reliable and reproducible methodology to geocode cryptosporidiosis notifications to fine-resolution spatial units (Census 2016 Small Areas), to enhance data validity and completeness, thus improving geospatial epidemiological studies.

Methods: A protocol was devised to utilise …

Playing At The Crossroads Of Religion And Law: Historical Milieu, Context And Curriculum Hooks In Lost & Found, Owen Gottlieb

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This chapter presents the use of Lost & Found – a purpose-built tabletop to mobile game series – to teach medieval religious legal systems. The series aims to broaden the discourse around religious legal systems and to counter popular depiction of these systems which often promote prejudice and misnomers. A central element is the importance of contextualizing religion in period and locale. The Lost & Found series uses period accurate depictions of material culture to set the stage for play around relevant topics – specifically how the law promoted collaboration and sustainable governance practices in Fustat (Old Cairo) in twelfth-century …

Biomolecules As Model Indicators Of In Vitro And In Vivo Cold Plasma Safety, Caitlin Heslin, Daniela Boehm, Brendan F. Gilmore, Julianne Megaw, Theresa A. Freeman, Noreen Hickok, Patrick J. Cullen, Paula Bourke

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The potential applications for cold plasma in medicine are extensive, from microbial inactivation and induction of apoptosis in cancer cells to stimulating wound healing and enhancing the blood coagulation cascade. The safe bio-medical application of cold plasma and subsequent effect on complex biological pathways requires precision and a distinct understanding of how physiological redox chemistry is manipulated. Chemical modification of biomolecules such as carbohydrates, proteins, and lipids treated with cold plasma have been characterized, however, the context of how alterations of these molecules affect cell behavior or in vivo functionality has not been determined. Thus, this study examines the cytotoxic …

Silver(I) And Copper(Ii) Complexes Of 1,10-Phenanthroline-5,6-Dione Against Phialophora Verrucosa: A Focus On The Interaction With Human Macrophages And Galleria Mellonella Larvae, Marcela Q. Granato, Thaís P. Mello Thaís P. Mello, Renata S Nascimento, Marcos D. Pereira, Thabatta L.S.A. Rosa, Maria C.V. Pessolani, Malachy Mccann Malachy Mccann, Michael Devereux, Marta H. Branquinha

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Phialophora verrucosa is a dematiaceous fungus that causes mainly chromoblastomycosis, but also disseminated infections such as phaeohyphomycosis and mycetoma. These diseases are extremely hard to treat and often refractory to current antifungal therapies. In this work, we have evaluated the effect of 1,10-phenanthroline-5,6-dione (phendione) and its metal-based complexes, [Ag (phendione)2]ClO4 and [Cu(phendione)3](ClO4)2.4H2O, against P. verrucosa, focusing on (i) conidial viability when combined with amphotericin B (AmB); (ii) biofilm formation and disarticulation events; (iii) in vitro interaction with human macrophages; and (iv) in vivo infection of Galleria mellonella larvae. The combination of AmB with each of the test compounds promoted …

Advancing Our Understanding Of Corneal Herpes Simplex Virus-1 Immune Evasion Mechanisms And Future Therapeutics, Emily Greenam, Sophie Gallagher, Rana Khalil, Conor C. Murphy, Joan Ní Gabhann-Dromgoole

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Herpes stromal keratitis (HSK) is a disease that commonly affects the cornea and external eye and is caused by Herpes Simplex Virus type 1 (HSV-1). This virus infects approximately 66% of people worldwide; however, only a small portion of these people will develop symptoms in their lifetime. There is no cure or vaccine available for HSV-1; however, there are treatments available that aim to control the inflammation caused by the virus and prevent its recurrence. While these treatments are beneficial to those suffering with HSK, there is a need for more effective treatments to minimise the need for topical steroids, …

Beyond Plea Bargaining: A Theory Of Criminal Settlement, Richard Lorren Jolly, J.J. Prescott

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Settlement is a term rarely used in criminal law. Instead, people speak almost exclusively of plea bargaining—i.e., enforceable agreements in which a defendant promises to plead guilty in exchange for a prosecutor’s promise to seek leniency in charging or at sentencing. But a traditional plea agreement is just the most visible instance of a much broader class of possible criminal settlement agreements. In terms of their fundamentals, criminal settlements are indistinguishable from their civil counterparts: through either an atomized or comprehensive bargain, parties exchange what they have for what they want, advancing their respective interests in cost minimization, risk mitigation, …

Bridges To A New Era: A Report On The Past, Present, And Potential Future Of Tribal Co-Management On Federal Public Lands, Monte Mills, Martin Nie

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Deep ancestral and traditional connections tie many Native Nations to the federal government’s public lands. The removal of these lands from indigenous control, their acquisition by the federal government, and the federal government’s approach to their management are largely premised upon the erasure or marginalization of those connections. Both physically and legally, Indian tribes have been removed from the landscapes they occupied since time immemorial. Rather than centering, honoring, and using those connections, the current discussion of tribal co-management of federal public lands is mostly bereft of this full legal and historical context.

Compounding these limitations is the considerable discretion …

The Automated Administrative State: A Crisis Of Legitimacy, Ryan Calo, Danielle Keats Citron

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The legitimacy of the administrative state is premised on our faith in agency expertise. Despite their extra-constitutional structure, administrative agencies have been on firm footing for a long time in reverence to their critical role in governing a complex, evolving society. They are delegated enormous power because they respond expertly and nimbly to evolving conditions. In recent decades, state and federal agencies have embraced a novel mode of operation: automation. Agencies rely more and more on software and algorithms in carrying out their delegated responsibilities. The automated administrative state, however, is demonstrably riddled with concerns. Legal challenges regarding the denial …