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A Profile Of The Victim Of Father-Daughter Incest: Testing Hypotheses From The Literature, Lawrence Manley Wilcox
A Profile Of The Victim Of Father-Daughter Incest: Testing Hypotheses From The Literature, Lawrence Manley Wilcox
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The purpose of this paper was to develop a profile of the victim of father-daughter incest. One descriptive profile was constructed from the literature, using a content analysis, and one profile was developed utilizing 25 subjects' responses to a questionnaire and a Minnesota Multiphasic Personality Inventory. These two profiles were compared. The items on the profiles with the greatest amount of predictive value were identified. A high point profile of the 4-8/8-4 scales on the MMPI was also identified in both the subjects' responses and in the literature. The final profile is designed for use by clinicians who work with …
Hormonal Control Of Phloem Loading In Celery (Apium Graveolens L.), Mark Watts
Hormonal Control Of Phloem Loading In Celery (Apium Graveolens L.), Mark Watts
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Previous research has indicated that phloem loading may be regulated by plant growth regulators. This conclusion, however, is based on circumstantial evidence in that previous experiments implemented complex tissue such as leaf discs or petioles to simulate phloem loading. Celery is a favorable system to measure phloem loading because it utilizes pure vascular tissue and/or isolated phloem tissue. Pure phloem tissue has not been used to date in phloem loading research.
It was demonstrated that the plant growth regulators gibberellic acid (GA3) and indole acetic acid (IAA) caused an enhancement of sucrose-loading in vascular bundles and in isolated …
Pantothenic Acid Status Of Adolescents, Brenda Ringer Eissenstat
Pantothenic Acid Status Of Adolescents, Brenda Ringer Eissenstat
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Information on human needs for pantothenic acid is limited and no recommended daily allowance has been established; although a safe and adequate level of 4-7 mg/day has been suggested for adults and adolescents. The adolescent population is often at risk for nutritional problems because of unusual eating patterns and major physiological changes which occur at this time. Pantothenic acid levels in urine, whole-blood and erythrocytes were determined in a healthy adolescent population using radioimmunoassay techniques. Dietary intakes were calculated from 4 day diet records and evaluated using a computer data base developed at Utah State University in conjunction with USDA. …
Manufacture Of White Soft Cheese From Ultrafiltered Whole Milk Retentate, Khalid Mohamed Shammet
Manufacture Of White Soft Cheese From Ultrafiltered Whole Milk Retentate, Khalid Mohamed Shammet
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Manufacture of white soft cheese from ultrafiltered whole cows• milk involved acidification of pasteurized homogenized whole milk to pH 6.0 with phosphoric or citric acid. The preacidified milk was ultrafiltered at 54 °C until 60% of original milk weight was removed as permeate, diafiltered with deionized water equal to 38.5% of the original milk and concentrated by UF (4.8 fold) to pre-cheese (38% total solid). The pre-cheese was heated to 76.7°C/16 sec, 71 .l°C/l6 sec (HTST) and 7l.l°C/l5 min (controlled water bath), inoculated with 2% starter culture (Streptococcus cremoris), renneted (10 ml/100 lb retentate) and placed in one …
A Neuropsychological Approach For Differentiating The Residual Effects Of Neonatal Intraventricular Hemorrhage, Glenn Thomas Goodwin
A Neuropsychological Approach For Differentiating The Residual Effects Of Neonatal Intraventricular Hemorrhage, Glenn Thomas Goodwin
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
It is well documented in the literature that low-birth-weight (LBW) and prematurity are associated with a variety of developmental disabilities. Within this population of LBW children it is estimated that at birth, up to 45% of them experience intraventricular hemorrhage (IVH). Only recently has pediatric research begun to look at the potentially unique effects of IVH, and attempt to separate these out form the effects of LBW in general. The purpose of this study was to investigate the neuropsychological differences that may occur in children with a history of mild or sever IVH, who are now approaching school age. The …
Effect Of Spring Grazing By Cattle On Growth And Survival Of Shrub Seedlings Interplanted In Crested Wheatgrass, Lynn M. Mcconville
Effect Of Spring Grazing By Cattle On Growth And Survival Of Shrub Seedlings Interplanted In Crested Wheatgrass, Lynn M. Mcconville
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
A short duration-high intensity grazing trial was conducted in spring of 1985 to determine whether shrub establishment in crested wheatgrass pastures could be enhanced through spring grazing by cattle, or by fertilization of shrub seedlings. Data was collected on diet selection by cattle during spring grazing, and on subsequent shrub growth and survival. Shrubs studied were transplants of sagebrush, Artemisia tridentata, fourwing saltbush, Atriplex canescens, bitterbrush, Purshia tridentata, and one year old bitterbrush seedlings.
Cattle did not browse sagebrush or fourwing saltbush transplants, but utilization of bitterbrush transplants and seedlings averaged 15 and 42 percent, respectively. Shrub …
Habit Reversal Treatment Of Bulimia Nervosa, Ken Small
Habit Reversal Treatment Of Bulimia Nervosa, Ken Small
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Although several writers have emphasized the habit-like characteristics of bulimia nervosa, no study has investigated the extent to which bulimia responds to treatment specifically for habit disorders. Habit reversal is a general treatment plan which teaches individuals to regain lost awareness of all aspects of a habit. The habit is then interrupted at the earliest movement in the chain by a physically competing response. Habit reversal (Azrin & Nunn, 1973) has been effective in treating other habits (e.g., stuttering, tics, bruxism). In the present study, habit reversal was adapted for the treatment of bulimia.
In response to newspaper advertisements, ten …
Factors Affecting Feeding Injury To Grasses By Adult Billbugs (Coleoptera: Curculionidae), Dale C. Nielson
Factors Affecting Feeding Injury To Grasses By Adult Billbugs (Coleoptera: Curculionidae), Dale C. Nielson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Factors associated with feeding injury to grass plants by two species of adult billbugs, Sphenophorus gentilis and S. parvulus, were evaluated. Early season tests utilized adult bluegrass billbugs while later studies involved wildrye billbugs. Types of feeding injury were determined and preferred feeding locations on host plants were identified for each billbug species. Greenhouse and field studies compared different species of grasses, individual plants within a species, and plants from different locations, for billbug susceptibility. The effect of grass plant age and stem size were also tested using bluegrass billbugs.
Using analysis of variance and multiple comparison tests, significant …
Cellular Immunity In Children With Down Syndrome (Trisomy-21), Roger Lee Noble
Cellular Immunity In Children With Down Syndrome (Trisomy-21), Roger Lee Noble
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Individuals with Down syndrome (US) suffer from increased incidence of respiratory infections and lymphoblastic leukemia, and a death rate that is particularly high in the first 5 years of life. Relatively few studies have probed immune parameters in young US children. Primary immune defects in DS may be masked by a degree of immune maturity in adults, and hygienic factors may have an effect on immune capability throughout the years. A study of young children can give clearer evidence of the actual primary immune defects in DS.
Blood samples were drawn from 20 DS children under 6 years old and …
Slow Rate Sand Filtration With And Without Clinoptilolite: A Comparison Of Water Quality And Filtration Economics, Gordon P. Foreman
Slow Rate Sand Filtration With And Without Clinoptilolite: A Comparison Of Water Quality And Filtration Economics, Gordon P. Foreman
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Slow rate sand filtration (SSF) amended with a 20 cm surface layer of clinoptilolite, a natural zeolite, was compared to SSF with no amendment in a field scale SSF facility treating 85 m3/d of water. Parameters examined included turbidity, coliforms, and ammonium removal. The control filter with sand and the experimental filter amended with the zeolite were also compared with respect to duration of filter cycle, cold weather operation, and economics.
Amended and unamended filters were approximately equivalent with respect to ammonium and coliform removal at 10° C. The zeolite amended cell was superior to the unamended cell …
Growth And Water Relations Of Mountain Big Sagebrush On Reclaimed Mine Soils In Southwestern Wyoming, Alan T. Carpenter
Growth And Water Relations Of Mountain Big Sagebrush On Reclaimed Mine Soils In Southwestern Wyoming, Alan T. Carpenter
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Mined-land reclamation practices in shrub-steppe ecosystems can be augmented by planting seedlings of locally dominant shrubs, e.g., mountain big sagebrush. Dispersion pattern could affect sagebrush performance by influencing amounts of windborne snow, soil and litter which accumulate around shrubs and by influencing water withdrawal by roots. Mountain big sagebrush seedlings were planted in plots on a reclaimed coal strip mine in two dispersion patterns: singly and in clumps of four at the same overall density.
Performance of mountain big sagebrush was monitored during two growing seasons. Measures included plant survival, end-of-growing season aboveground biomass, leaf water potential components, soil water …
Chemical Weathering Of Pyrite In Soils, Aaron D. Brown
Chemical Weathering Of Pyrite In Soils, Aaron D. Brown
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The products of pyrite oxidation, including solution phase Fe2+, Fe3+, S2O32-, S4O62-, SO32- and SO42- and solid phase Fe(OH)3, were measured under controlled conditions in order to investigate the behavior of pyrite in calcareous and alkaline soils.
The distribution of sulfur oxidation products is pH dependent and can be interpreted in terms of metastable equilibrium among thiosulfate, disulfane disulfonate and sulfite. Thiosulfate and sulfite predominate in the pH range greater than about pH 7 or 8. Sulfane disulfonates are more …
Effect Of Iron On Residual Nitrite Level In Ground Pork And Model Systems, Changmin Kim
Effect Of Iron On Residual Nitrite Level In Ground Pork And Model Systems, Changmin Kim
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The effects of iron, temperature, and presence of botulinal spores or sodium ascorbate on depletion of nitrite level were determined in meat and model systems. Higher temperature and presence of botulinal spores definitely increased nitrite depletion in a meat system. Added hemoglobin also significantly increased nitrite depletion, while ferrous iron and ferric iron did not significantly decrease nitrite level in a meat system. High temperature and presence of sodium ascorbate increased nitrite depletion in a model system. Only ferrous iron significantly decreased nitrite level in the absence of ascorbate, while ferric iron, heme iron, and ferritin iron did not decrease …
Seasonal Feeding Behavior And Forage Selection By Goats In Cleared And Thinned Deciduous Woodlands In Northeast Brazil, Roberto Cesar Magalhaes Mesquita
Seasonal Feeding Behavior And Forage Selection By Goats In Cleared And Thinned Deciduous Woodlands In Northeast Brazil, Roberto Cesar Magalhaes Mesquita
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The seasonal feeding behavior, forage preferences and body weight responses of goats were studied under three densities of woodland (called caatinga), and under three stocking rates. The experiment was located in the semi-arid tropics of northeastern Brazil at 3 42' South latitude, and 40 21' West longitude at an elevation of 75 meters. Mean annual precipitation of the area is 832 mm.
Removing the shrubs and trees increased yields of herbaceous only on partially-cleared sites. Goats gained body weight (kg BW/ha) during the wet season, with the cleared treatment showing the best body weight response per unit of land. However …
Optimal Forest Rotation: Decisions Under Conditions Of Certainty And Uncertainty, Rabindra Nath Bhattacharyya
Optimal Forest Rotation: Decisions Under Conditions Of Certainty And Uncertainty, Rabindra Nath Bhattacharyya
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The existing literature determining the optimal rotation period of a forest stand under conditions of certainty, as well as under uncertainty, lacks the genera 1 scope to be useful. A forest provides timber of commercial value, a flow of recreational services, and other valuable environmental services. Providing goods and services involves benefits as well as costs. Relevant management decisions depend on the net values that can be obtained. The present work developes a more general model for determining an optimal rotation period incorporating various fixed and variable costs associated with timber production and recreational services in an environment of certainty …
Decisions Of Pregnant Adolescents As They Affect Later Well-Being, Myra Lynch
Decisions Of Pregnant Adolescents As They Affect Later Well-Being, Myra Lynch
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The purpose of this study was to examine the quality of family life presently experienced by women who were premaritally pregnant as teenagers. Self-esteem and general life satisfaction were measured in order to evaluate the results of pregnancy decisions made five to 10 years earlier. A survey design was used to (1) obtain demographic data, (2) analyze the relationships between these women and their parents, (3) determine the level and type of education completed by the women, (4) compare mothers who married before the birth of the child, single mothers, and mothers releasing their children for adoption on many variables …
A Comparison Between Desensitization And Relaxation Training In The Treatment Of Primary Dysmenorrhea, Susan Myrna Jones Carcelli
A Comparison Between Desensitization And Relaxation Training In The Treatment Of Primary Dysmenorrhea, Susan Myrna Jones Carcelli
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The use of relaxation, desensitization, and relaxation plus desensitization in the treatment of primary dysmenorrhea was investigated in this study. Subjects were 45 university women who experienced either congestive or spasmodic dysmenorrhea. Each subject was individually treated in four, one-hour sessions during the first 20 days of her menstrual cycle. Subjects were divided into three groups: Group 1 obtained four hours of progressive relaxation training, group 2 was asked to self-relax while being administered scenes from a standardized menstrual hierarchy, and group 3 obtained both relaxation training and desensitization. Type of dysmenorrhea was assessed by the Menstrual Symptom Questionnaire (MSQ). …
The Relationship Of Type A And Type B Coronary Behavior Patterns And Achievement Striving, Jolene Lowry Adams
The Relationship Of Type A And Type B Coronary Behavior Patterns And Achievement Striving, Jolene Lowry Adams
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The purpose of this study was to conduct a systematic replication of the experiment of Burnam, Pennebaker, and Glass (1973) using an adult population and the Jenkins Activity Survey. Additionally, this study attempted to address the issue of whether the previous results would be substantiated when a non-college sample was used.
The subjects consisted of40 females and 40 males who volunteered to participate in the study. All subjects were given the Jenkins Activity Survey and randomly assigned to Condition I, the No Deadline condition, or Condition II, the Deadline condition. The subjects in the No Deadline condition were given arithmetic …
Production Of Monoclonal Antibodies Specific For The Microgametocytes Of Eimeria Tenella, Marc A. Laxer
Production Of Monoclonal Antibodies Specific For The Microgametocytes Of Eimeria Tenella, Marc A. Laxer
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The objective of this study was to produce a monoclonal antibody specific for the microgametocytes of Eimeria tenella, examine the site and stage specificity of the antibody, and investigate the immunopotency of the antibody. BALB/c mice were immunized with antigen containing Eimeria tenella microgametocytes isolated from in vitro systems. After three intraperitoneal immunizations with the antigen and one booster immunization administered by tail vein injection, the mice were sacrificed and their spleen cells fused with SP2/0 mouse myeloma cells using polyethylene glycol as a fusing agent. Resultant hybridomas were screened by immunoelectrophoresis, indirect immunofluorescent antibody assay, and immunoelectron microscopy …
A Comparison Of Eight Selected Sports Bras: Biomechanical Support, Overall Comfort Ratings And Overall Support Ratings, Lajean R. Lawson
A Comparison Of Eight Selected Sports Bras: Biomechanical Support, Overall Comfort Ratings And Overall Support Ratings, Lajean R. Lawson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The purpose of this paper was to evaluate eight currently marketed sports bras for differences in control of vertical displacement of the breast, overall comfort scores, and overall support scores for A, B, C and D cup sizes. An additional goal was to determine and compare differences in the vertical displacement of the nude breast among the four cup size groups. To determine differences in vertical displacement, subjects were filmed while jogging on a treadmill, and film data were analyzed frame by frame. To determine differences in overall comfort and support ratings, subjects tested each of the eight bra styles …
Energetics Of The American Kestrel (Falco Sparverius) During Three Seasons In Northern Utah, Lucinda Haggas
Energetics Of The American Kestrel (Falco Sparverius) During Three Seasons In Northern Utah, Lucinda Haggas
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Behavioral activities and predatory behavior of 18 American Kestrels (Falco sparverius, 9 males and 9 females) were observed for 350+ hours during 3 seasons (nonbreeding = Jan-Feb , breeding = mid-Mar-Apr, and postbreeding = late-Aug-Sept) in northern Utah. Daily energy expenditure (DEE) of male and female kestrels was estimated with a model that incorporated flight activity data from free-living birds and laboratory measurements on daytime and nighttime metabolic rates and energy costs of tissue production derived from captive kestrels. Production costs were included in the DEE for breeding and postbreeding kestrels.
The energy cost of gonadal growth for males (0.02 …
Predicting Digestibilities Of Alfalfa Hays With Near Infrared Reflectance Spectroscopy, David H. Clark
Predicting Digestibilities Of Alfalfa Hays With Near Infrared Reflectance Spectroscopy, David H. Clark
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Forty-four alfalfa hays from different cuttings, maturities, and locations were fed to sheep in a digestion study. Subsamples of the hays along with corresponding fecal samples were ground and analyzed for dry matter, (DM), organic matter (OM), crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and permanganate lignin. In vivo digestibility (IVDMD) were also determined fro each hay.
The hay and fecal samples were scanned with a near infrared reflectance spectrophotometer. Chemical and digestible data for each hay and fecal sample were entered into the computer and separate multiple regression equations developed.
Fifteen other alfalfa hays with …
Behavioral Response Of Desert Bighorn Sheep To Human Harassment: A Comparison Of Disturbed And Undisturbed Populations, Michael M. King
Behavioral Response Of Desert Bighorn Sheep To Human Harassment: A Comparison Of Disturbed And Undisturbed Populations, Michael M. King
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Desert bighorn sheep (Ovis canadensis nelsoni) response to human disturbance was evaluated in southeastern Utah from 1981-1983. Bighorn response was compared between the Red Canyon area, an area with relatively high levels of human disturbance, and the White Canyon area, an area with relatively low levels of human disturbance. Bighorn were deliberately harassed by vehicles and hikers and immediate response and distance fled were recorded. When bighorn remained in the presence of the harassing stimuli, actual time spent in and proportion of animals engaged in various behaviors were recorded to determine group wariness and activity budgets under harassed …
Parameters Controlling Sediment Composition Of Modern And Pleistocene Jamaican Reefs, Stephen K. Boss
Parameters Controlling Sediment Composition Of Modern And Pleistocene Jamaican Reefs, Stephen K. Boss
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Recent carbonate sediments from Jamaican north coast fringing reefs display variation in constituent composition, texture, and mineralogy related to their location on the reef. Samples were collected along lines which traversed the back reef and fore reef (0.5m to 70m).
The sediment is dominated by highly comminuted coral fragments, plates of the calcareous green alga, Halimeda, coralline algae, and the encrusting Foraminifera, Homotrema rubrum, with lesser amounts of other taxonomic groups (Foraminifera; molluscs; echinoderms). Relative abundances of these biotic components vary between sites. Q-mode cluster analysis indicates that constituent composition can be used to delineate the different reef …
Phosphorus Dynamics In Dingle Marsh, Idaho, Rex C. Herron
Phosphorus Dynamics In Dingle Marsh, Idaho, Rex C. Herron
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Phosphorus mass-balance studies of Dingle Marsh, Idaho indicated that the marsh was an annual net sink for total suspended sol ids and all forms of phosphorus under consideration: total phosphorus, particulate phosphorus, total dissolved phosphorus, dissolved organic phosphorus, and orthophosphate phosphorus. During some months, however, more phosphorus was exported from the marsh than entered.
Phosphorus mass-balance dynamics were compared between night and day. Total suspended solids and particulate phosphorus dynamics were controlled by sedimentation of particulate material, a physical process, and were unaffected by light conditions. Dissolved organic and ortho-phosphorus were affected by light conditions indicating, at least indirectly, that …
An Autecological Study Of Dyers Woad (Isatis Tinctoria L.) On Utah Rangeland, Ann T. Fuller
An Autecological Study Of Dyers Woad (Isatis Tinctoria L.) On Utah Rangeland, Ann T. Fuller
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Northern Utah rangelands have become infested with dyers woad (Isatis tinctoria L.) and control is needed. Mechanical and chemical control on rangelands produce undesirable effects. A possible alternative is biological control, but information is not available on plant response to this control method. This autecological study and simulation of control methods examined dyers woad phenology, seed endurance and response to simulated grazing, hand rogueing and plowing. The phenology study documented dyers woad growth patterns from May 1982 to November 1983. During the first year, 65 percent of the seedlings died while the remaining 35 percent grew rosette leaves. During …
The Acarau Valley In Northeast Brazil: Vegetation, Soils And Land Use., Joao Stacishin De Queiroz
The Acarau Valley In Northeast Brazil: Vegetation, Soils And Land Use., Joao Stacishin De Queiroz
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The purpose of this study was to describe and characterize the principal kinds of land in the mid-portion of the Acarau Valley, Ceara State, Northeast Brazil. The specific aims were: (1) to develop a specific-purpose soil classification; (2) to investigate vegetation-environment relationships; (3) to develop hypotheses about short-term vegetation dynamics; and (4) to evaluate the usefulness of computer processed multispectral-scanner (MSS) data for mapping rangeland resources in Northeast Brazil.
Fifty-one soil profiles were described and ocular estimates of vegetation cover by species were made at each subjectively selected site. Two Landsat scenes were used to assess the usefulness of MSS …
Temporal And Spatial Partitioning Of The Soil Water Resource Between Two Agropyron Bunchgrasses And Artemisia Tridentata, Halldor Thorgeirsson
Temporal And Spatial Partitioning Of The Soil Water Resource Between Two Agropyron Bunchgrasses And Artemisia Tridentata, Halldor Thorgeirsson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Dynamics of soil water use by two cool-season Agropyron bunchgrasses during the warm season depletion of soil water reserves were monitored for two years in experimental plots in the field. Agropyron desertorum, an introduced, competitive species from Eurasia, extracted more water from the deeper ( > 50 cm) soil layers than the native, less competitive Agropyron spicatum. Agropyron desertorum both extracts this water earlier and to lower soil water potentials than Agropyron spicatum. From the water extraction dynamics of the grasses in monocultures and in their two-way (50:50) mixtures with a shrub they commonly co-occur with, Artemisia tridentata …
The Isolation, Property Analysis And Determination Of In Planta Growth Ability Of Extracellular Polysaccharide Mutants Of Corynebacterium Michiganense Pv Insidiosum, Mary C. Paschke
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Corynebacterium michiganense pv insidiosum, the organism responsible for bacterial wilt of alfalfa, produces an extracellular polysaccharide (EPS) that has been linked to its virulence. The EPS is composed of three different-sized molecules (components I, II and III). It has been demonstrated that this EPS accumulates in the vascular system of infected plants and causes disruption of the flow of water.
The importance of EPS to the survival and pathogenicity of C.m. pv insidiosumis not known. In order to begin assessing the value of EPS, mutant strains that are EPS-deficient were isolated after UV mutagenisis. Analysis by gel filtration …
Iron Requirement Of Clostridium Botulinum Type A And Characterization Of Iron-Sulfur Proteins In Nitrite Treated And Untreated Botulinal Cells, Divya Shree A. Reddy
Iron Requirement Of Clostridium Botulinum Type A And Characterization Of Iron-Sulfur Proteins In Nitrite Treated And Untreated Botulinal Cells, Divya Shree A. Reddy
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The effect of added iron on the growth of Clostridium botulinum type A in a chemically defined medium was studied. Growth of C. botulinum was supported by an iron level of 0.05 ug/ml with maximum growth observed at a level of 3 ug iron/ml.
Electron paramagnetic resonance (EPR) studies were conducted to detect the presence of iron-sulfur centers and iron-nitric oxide complexes in untreated and nitrite treated cell-free extracts of C. botulinum type A. Untreated extracts of C. botulinum exhibited EPR signals in the oxidized and reduced states characteristic of a "HiPiP-type" iron-sulfur center (g=2.02) in the oxidized state and …