Digital Commons Network^™

Controlling Nonspecific Adsorption Of Proteins At Bio-Interfaces For Biosensor And Biomedical Applications, Harshil D. Dhruv

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Partitioning of poly(ethyleneglycol) (PEG) molecules in 2-D and 3-D systems is presented as a self-assembly approach for controlling non-specific adsorption of proteins at interfaces.

Lateral restructuring of multi-component Langmuir monolayers to accommodate adsorbing proteins was investigated as a model 2-D system. Ferritin adsorption to monolayers containing cationic, nonionic, and PEG bearing phospholipids induced protein sized binding pockets surrounded by PEG rich regions. The number, size, and distribution of protein imprint sites were controlled by the molar ratios, miscibility, and lateral mobility of the lipids.

The influence of PEG chain length on the ternary monolayer restructuring and protein distribution was also …

Sustaining The Allideghi Grassland Of Ethiopia: Influences Of Pastoralism And Vegetation Change, Almaz Tadesse Kebede

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Allideghi Wildlife Reserve in the Amibara District of Afar Regional State, Ethiopia, has international significance for harboring endangered Grevy's Zebra and other wildlife dependent on grasslands. The reserve is increasingly used by pastoral people and their herds. Impacts of livestock on native vegetation include direct effects of grazing and indirect effects from livestock-facilitated dispersal of an invasive plant, Prosopis juliflora. The main research objective was to determine effects of pastoralism and vegetation change on prospects for sustaining the Allideghi Wildlife Reserve as grassland habitat for Grevy's Zebra. Methods included use of driving surveys to quantify resource use by …

Hydrologic Information Systems: Advancing Cyberinfrastructure For Environmental Observatories, Jeffery S. Horsburgh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Recently, community initiatives have emerged for the establishment of large-scale environmental observatories. Cyberinfrastructure is the backbone upon which these observatories will be built, and scientists' ability to access and use the data collected within observatories to address research questions will depend on the successful implementation of cyberinfrastructure. The research described in this dissertation advances the cyberinfrastructure available for supporting environmental observatories. This has been accomplished through both development of new cyberinfrastructure components as well as through the demonstration and application of existing tools, with a specific focus on point observations data. The cyberinfrastructure that was developed and deployed to support …

Predicting Rna Secondary Structures By Folding Simulation: Software And Experiments, Joel Omni Gillespie

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

We present a new method for predicting the secondary structure of RNA sequences. Using our method, each RNA nucleotide of an RNA Sequence is represented as a point on a 3D triangular lattice. Using the Simulated Annealing technique, we manipulate the location of the points on the lattice. We explore various scoring functions for judging the relative quality of the structures created by these manipulations. After near optimal configurations on the lattice have been found, we describe how the lattice locations of the nucleotides can be used to predict a secondary structure for the sequence. This prediction can be further …

Vegetation Characteristics Of Wyoming Big Sagebrush Communities Historically Seeded With Crested Wheatgrass In Northeastern Great Basin, Usa, Justin Rodney Williams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Crested wheatgrass (Agropyron cristatum [L.] Gaertn.) is one of the most commonly seeded grass species in the western United States and dominates thousands of hectares in the Great Basin. Although many degraded Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis) plant communities have been seeded with crested wheatgrass, successional pathways, influence of soil attributes, and cultivation history on the vegetation of these communities have not been fully characterized. I sought to identify community phases, vegetative differences, and soil attributes that explain variation among 35 Wyoming big sagebrush communities historically seeded with crested wheatgrass. All communities were more than …

Cognitive Inference And Resulting Behaviors In Response To Ambiguous Threat In The Coyote, Canis Latrans, Sarah Shawnee Dawson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

While antipredator strategies have been a focus of behavioral research for decades, scientists generally study the responses of prey toward overt, explicit threat. However, risk can also be significant when a threat is covert, such as when an ambush predator may be nearby or a secondary threat remains after a predator's departure. Little is known about the mechanism that prey use to assess risk in a predator's absence. Tests were conducted to determine the manner in which coyotes respond to these ambiguous threats. Specifically, I tested whether coyotes respond to prior anthropogenic activity that has occurred near their only food …

Mechanisms Of Induced Cell Death In Bluetongue Virus Challenged Human Cell Lines, Justin Darrel Hoopes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bluetongue virus (BTV) is a pathogenic member of the Reoviridae family. BTV does not cause disease in humans, but is capable of selectively infecting and killing certain transformed human cell lines. Understanding BTV's oncotrophism may lead to new therapeutics for treating cancer. This study focused on the underlying mechanisms of BTV-induced cell death in carcinoma cell lines. It was our hypothesis that BTV infects human carcinoma transformed cells, produces mRNA and protein, induces a strong inflammatory response, induces mitogen activated protein kinase (MAPK)-based pro-apoptotic signaling, inhibits PKB-based signaling, and eventually kills the cell by inducing apoptosis.

Three carcinoma cell lines …

Social Acceptability Of Conifer Control And Sagebrush Restoration In The Northern Rocky Mountain Region, Cameron G. Nay

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In the past two centuries, woody plant species have increased in density and extent throughout the rangelands of North America. This encroachment generally has undesirable effects on hydrological function, forest resources, and plant community composition. Encroachment of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa) into sagebrush communities is occurring in the Northern Rocky Mountain region. Removal and restoration treatments are often proposed to manage this issue, mainly prescribed fire, mechanical destruction, and/or herbicide use. Several contextual factors may affect public level of acceptability for such treatments. The issue frames used to present this problem to …

Application Of Electrified Fladry To Decrease Risk Of Livestock Depredation By Wolves (Canis Lupus), Nathan J. Lance

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wolf (Canis lupus) predation on livestock can cause economic and emotional hardships for livestock producers, complicating the balance of wolf conservation with other human interests. New management tools that decrease risk of predation may offer additional flexibility or efficiency for both livestock producers and management agencies. I examined 1) the efficacy of electrified fladry compared to fladry at protecting a food source from wolves in captivity, 2) the efficacy of electrified fladry for reducing wolf use of pastures and preventing depredations, and 3) the applicability of electrified-fladry. In captivity I tested the reaction from 15 groups (46 wolves) …

Taste Sensitivity To 6-N-Propylthiouracil (Prop) As A Biological Marker For Vulnerability To Stress In Mothers And Children, Deann Jones

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Some people are genetically more vulnerable to stress than others, leading them towards poorer outcomes following stressful events. Mothers' vulnerability to stress may, in turn, influence their children, leading their children towards poor outcomes as well. A biological marker of vulnerability to stress may indicate mothers who are at greater risk for experiencing parenting stress, depression, and less support of the infant's emotional development, and infants who are at greater risk for development of poor emotion regulation and behavior problems. Taste sensitivity to propylthiouracil (PROP) is proposed as a biological marker of stress vulnerability in mothers and children. This research …

Assessing The Effects Of The Holidays On Body Composition And Weight Change Using Air Displacement Plethysmography, Jessyka Nicholle Larson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to determine the change in weight and body composition over a 6-week holiday period. Change in body composition was measured using air displacement plethysmography technology (Bod Pod^®). Thirteen men and 21 women ranging in age from 23-61 years were participants for this study. Baseline testing occurred the week of Thanksgiving Day (November 24 or 25), and the post-holiday assessment was the week after Utah State University's holiday break (January 5 or 6). Results from a nutrition and exercise questionnaire revealed a significant difference between pre-holiday and post-holiday consumption of vegetables (8.6 ± 8.3 vs. …

Evaluation Of Macroinvertebrates As A Food Resource In The Assessment Of Lotic Salmonid Habitat, Nicholas P. Weber

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Criteria used to characterize lotic salmonid habitat are often based on observed correlations between physical habitat characteristics and salmonid abundances. A focus on physical habitat features ignores other habitat components, such as an adequate supply of food that set the physiological limitations on salmonid growth and survival. This study outlines the development of a habitat assessment approach that focuses on how invertebrate food availability interacts with stream temperatures to determine salmonid growth potentials. Abundances of benthic and drifting invertebrate communities, stream temperatures, and juvenile steelhead trout (Onchorhynchus mykiss gairdneri) summer growth rates and abundances were measured within 10 …

Biological And Economic Effects Of Grazing Spring-Calving Cow-Calf Pairs On Improved Irrigated Pastures Using Creep Supplementation, Adam F. Summers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Recent trends to develop farmland into improved irrigated pastures raise questions regarding the profitability of creep supplementing terminal-sired calves on these production systems. This study was initiated to answer these questions. Two previously established adjacent sprinkler-irrigated plots were separated into 2 paddocks. One plot (3.4 ha) consisted of a monoculture of Seine tall fescue while the other plot (3.9 ha) consisted of a mixture of Seine tall fescue, AC Grazeland Alfalfa, and Norcen birdsfoot trefoil. The mixture of the second plot consisted of 50% tall fescue, 37.5% alfalfa, and 12.5% birdsfoot trefoil. Plots were designated as monoculture no-creep supplement (MONOC) …

Production Performance And Profiles Of Milk Fatty Acids Of Lactating Dairy Cows Fed Whole Safflower Seed Containing High Fat And Low Fiber, Christopher M. Dschaak

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Oil seeds are natural sources of fat and protein in diets for lactating cows, and are usually fed whole or crushed. A recently released variety of safflower seed, 'Nutrasaff,' contains high fat (47% crude fat) and low fiber (26% NDF), and has a potential to be effectively used as a fat supplement for lactating dairy cows. Therefore, a lactating dairy cow trial was conducted to assess production performance of dairy cows when fed graded levels of whole Nutrasaff safflower seed (NSS), to determine the optimum level of NSS supplementation in the diet and to identify its impact on milk fat …

Design And Evaluation Of Oligonucleotide Microarrays For The Detection Of Bovine Pathogens, Ryan Weldon Black

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two microarray designs were developed and produced to screen for multiple bovine pathogens commonly found in the cattle industry today. The first microarray was designed, built, and processed in-house using conventional material and equipment and targeted Pasteurella multocida, Manheimia haemolytica, Histophilus somni, and Arcanobacterium pyogenes. For each pathogen, 12 perfect-match oligonucleotide probes, which were also designed in-house, targeted different sections of the respective 16S ribosomal genes, and were coupled with 12 corresponding mismatched probes for background. These arrays were able to produce distinct hybridization patterns for each pathogen that were easily visible without the need for …

The Use Of Reverse Genetics To Clone And Rescue Infectious, Recombinant Human Parainfluenza Type 3 Viruses, Jason Peter Roth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Reverse genetics is a discipline that involves the use of genetic manipulation and modification to study an organism's altered phenotype. In this study, infectious recombinant viruses were rescued from altered cDNA clones encoding the antigenome of human parainfluenza virus type 3 and the resulting phenotypes were examined. In one clone, the gene for the enhanced green fluorescent protein was inserted into the virus antigenome to be expressed during viral replication, resulting in infected cells emitting green fluorescence. Viral titers, mRNA replication, and genomic replication for the virus expressing the enhanced green fluorescent protein were reduced when compared to the human …

Punta Toro Virus Infection In Mice: Strain Differences In Pathogenesis And Regulation Of Interferon Response Pathways, Michelle Mendenhall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Adames strain of Punta Toro virus (PTV-A) causes acute hepatic disease in hamsters and mice similar to that seen in natural Rift Valley fever virus (RVFV) infection, while the Balliet strain (PTV-B) is apathogenic. The ability of PTV-A to suppress the interferon (IFN) response has been demonstrated in hamsters and is thought to be a contributing factor to PTV-A's pathogenicity in hamsters. PTV-B is not assumed to exhibit this IFN-antagonistic activity, as it stimulates production of significantly higher IFN-β levels. To elucidate the role of IFN in resistance of mice to PTV-B infection, we utilized mice deficient in a …

School-Based Services For Children With Attention-Deficit/Hyperactivity Disorder, Tonya M. Tree

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was designed to present descriptive data from a survey of 201 school psychologists. Psychologists completed a survey addressing current practices for determining placement for students with ADHD including the role of school psychologists in determining placement, how often and who monitors services, where students are served, and what services are provided in each setting. Findings indicated that psychologists were involved less frequently in placement decisions and evaluation for Section 504 than in Special Education. Students with 504 plans received less frequent follow-up than students in Special Education. Results indicated that schools were generally following federal guidelines and recommendations …

Lessons In Humanity: A Memoir, Chelsi Joy Sutton-Linderman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In the opening pages of his work, Dog Years; A Memoir, Mark Doty explains: Love for a wordless creature, once it takes hold, is an enchantment, and the enchanted speak, famously, in private mutterings, cryptic riddles, or gibberish. This is why I shouldn't be writing anything about the two dogs that have been such presences for sixteen years of my life. How on earth could I stand at the requisite distance to say anything that might matter? (1)

In this thesis I argue that Doty, among other respected contemporary writers, is saying something that matters when he writes of …

Evaluation Of An Interactive Health Communication Trans Fat Website, Tara Banks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In order to evaluate the short-term educational and behavioral impact of the American Heart Association's "Face the Fats" web application had upon college students, a study involving 116 Utah State University undergraduate students was conducted. A one-group pre-posttest design was utilized to answer eight research questions focusing on: health risks associated with trans fat, general trans fat knowledge, ability to identify foods containing trans and saturated fats, food label information, healthy alternatives to trans fat, and readiness to change trans fat intake. Participants completed an online pretest survey and then viewed the "Face the Fats" website at their own pace. …

Evolutionary Genetics Of Tetrodotoxin (Ttx) Resistance In Snakes: Tracking A Feeding Adaptation From Populations Through Clades, Chris R. Feldman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Understanding the nature of adaptive evolution has been the recent focus of research detailing the genetic basis of adaptation and theoretical work describing the mechanics of adaptive evolution. Nevertheless, key questions regarding the process of adaptive evolution remain. Ultimately, a detailed description of the ecological context, evolutionary history, and genetic basis of adaptations is required to advance our understanding of adaptive evolution. To address some of the contemporary issues surrounding adaptive evolution, I examine phenotypic and genotypic changes in a snake feeding adaptation.

Adaptations can arise through fixation of novel mutations or recruitment of existing variation. Some populations of the …

Treatment Of Saltcedar (Tamarix Spp.): Economics And Feasibility, Christopher L. Thompson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The invasive species Saltcedar is affecting water and land resources throughout the western states of America. Because of great water use capabilities and other ecosystem detriments, Saltcedar has been targeted for treatment.

For successful management of Saltcedar, individual landowners need to be aware of the costs and benefits of treating Saltcedar. Eleven of the most commonly reported treatment methods were evaluated for firm level economic feasibility. Evaluated on the basis of treatment cost, treatment effectiveness, Saltcedar water-use, and re-vegetation water-use, a production plan of ten years was created for each treatment method. Some treatment methods required re-treatment and were evaluated …

Self-Medicative Behavior Of Sheep Experiencing Gastrointestinal Nematode Infections And The Postingestive Effects Of Tannin, Larry D. Lisonbee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Diet selection and self-medication are fundamental to the survival of all species. The abilities to choose healthy foods in response to past consequences are basic elements of evolution. This study explores self-medication regarding tannins both as a medication and as a dietary challenge. In the first study, sheep with natural parasite infections were offered a low quality supplement containing a dose of tannins considered to be therapeutic (medicine), while the control infected lambs received the same supplement without tannins (placebo). This study included a group of parasite-free lambs. The parasitized lambs ate more of the tannin containing supplement than non-parasitized …

A Study Of Utah Teachers' Developmentally Appropriate Beliefs And Practices As Related To Perceptions Of Kindergarteners' Successful School Entry, Mary Mceuen Darnell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was an exploration of 450 Utah kindergarten teachers' perceptions of problems children face at the time of kindergarten entry, as well as an examination of the teachers' developmentally appropriate beliefs and practices. Consistent with previous research, teachers' beliefs were found to be more developmentally appropriate than their reported practices. This study also investigated the relationship between both teacher and classroom/school demographics and teachers' developmentally appropriate beliefs and practices. Further, the relationship between teachers' perceptions of children's transition to kindergarten and beliefs, practices, teacher demographics, and classroom/school demographics was studied.

Study findings indicated that teachers perceive 20% of kindergarten …

Agroforestry Practice Adoption Among Solomon Island Women On The Island Of Malaita, Etta K. Sechrest

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The goal of agricultural training is the adoption and diffusion of introduced agriculture techniques. New subsistence agricultural techniques have been introduced mainly to the male population in many developing countries, even though most subsistence farmers are women. Therefore, an understanding of how new subsistence agricultural techniques can be introduced and adopted by women would be important to achieve. This study focuses on women's adoption of agricultural techniques. It takes place on the island of Malaita, in the Solomon Islands. The study looks at the adoption of agroforestry and several other subsistence techniques that were introduced under a joint program by …

A Conceptual Framework For Understanding Effects Of Wildlife Water Developments In The Western United States, Randy T. Larsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Free water can be a limiting factor to wildlife in arid regions of the world. In the western United States, management agencies have installed numerous, expensive wildlife water developments (e.g. catchments, guzzlers, wells) to: 1) increase the distribution or density of target species, 2) influence animal movements, and 3) mitigate for the loss of available free water. Despite over 50 years as an active management practice, water developments have become controversial for several species. We lack an integrated understanding of the ways free water influences animal populations. In particular, we have not meshed understanding of evolutionary adaptations that reduce the …

Synthetic Complexes Of Relevance To Ni(Ii)-Containing Enzymes, Katarzyna Rudzka

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The work outlined herein presents an investigation of synthetic model complexes of relevance to the active sites of Ni(II)-containing enzymes, particularly urease, glyoxalase I, and acireductone dioxygenase. The research focuses on studying the structural and reactivity features of nickel complexes with biologically relevant substrates. The anion of acetohydroxamic acid is a well-known inhibitor of urease enzymes, including those isolated from Klebsiella aerogenes and Bacillus pasteurii. A precursor to the acetohydroxamate coordination in ureases is proposed to be an interaction between Ni(II) and acetohydroxamic acid. By using a novel supporting chelate ligand capable of secondary hydrogen bonding interactions a novel …

Family Involvement In Three Utah Adolescent Residential Treatment Centers, Wesley W. Larson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Family participation in residential treatment for disturbed adolescents has increased over time. A general sense of this movement is that this is beneficial. However, there are no common descriptions of family involvement in residential treatment in the literature. In order to be able to better understand which components of family involvement are most beneficial, we need to first understand how residential treatment centers (RTCs) define and describe family involvement. This study compiled data from interviews with nine participants, one each from administration, therapy staff, and direct care staff level of three northern Utah RTCs that claimed family involvement in youth …

A Qualitative Study Of Seminary Principals For The Church Of Jesus Christ Of Latter-Day Saints, Eric W. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This qualitative study investigates how leadership is embodied within the role of seminary principals in released-time seminaries for The Church of Jesus Christ of Latter-day Saints. This study utilized a grounded theory approach and constant comparative analysis while triangulating the data obtained from personal interviews, participant observations, and analysis of documents. The primary sources of data came from the personal experiences and perspectives of four principals, eight teachers, and one area administrator that are analyzed through biographical interviews.

Analyses of the data were completed to determine common themes of leadership that were embodied by principals in released-time seminaries for The …

Reported Mental Health Issues And Marital Quality: A Statewide Survey, Joseph Ruben Smart

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study included a representative random sample of 886 married individuals in Utah. This sample was surveyed to discover the relationship between demographic variables, reported mental health issues, and marital quality. In addition, this study sought to discover models, using demographic variables and reported mental health issues, to predict for separate dimensions of marital quality. This survey was a replication of a study completed primarily in Oklahoma, with the addition of questions about the participants' mental health.

Spearman's rho, Pearson's R, and multiple regression were used to analyze the data. The results of the study show that: religious beliefs …