Digital Commons Network^™

Urine Cell-Free Dna Multi-Omics To Detect Mrd And Predict Survival In Bladder Cancer Patients, Pradeep S Chauhan, Alexander Shiang, Irfan Alahi, Wenjia Feng, Cayce Nawaf, Kevin Chen, Peter K Harris, Faridi Qaium, Casey Hatscher, Anna Antiporda, Lindsey Brunt, Lindsey R Mayer, Brian C Baumann, Eric H Kim, Melissa A Reimers, Zachary L Smith, Aadel A Chaudhuri, Et Al.

2020-Current year OA Pubs

Circulating tumor DNA (ctDNA) sensitivity remains subpar for molecular residual disease (MRD) detection in bladder cancer patients. To remedy this problem, we focused on the biofluid most proximal to the disease, urine, and analyzed urine tumor DNA in 74 localized bladder cancer patients. We integrated ultra-low-pass whole genome sequencing (ULP-WGS) with urine cancer personalized profiling by deep sequencing (uCAPP-Seq) to achieve sensitive MRD detection and predict overall survival. Variant allele frequency, inferred tumor mutational burden, and copy number-derived tumor fraction levels in urine cell-free DNA (cfDNA) significantly predicted pathologic complete response status, far better than plasma ctDNA was able to. …

Semantic Wikis As Flexible Database Interfaces For Biomedical Applications, Marco Falda, Manfredo Atzori, Maurizio Corbetta

2020-Current year OA Pubs

Several challenges prevent extracting knowledge from biomedical resources, including data heterogeneity and the difficulty to obtain and collaborate on data and annotations by medical doctors. Therefore, flexibility in their representation and interconnection is required; it is also essential to be able to interact easily with such data. In recent years, semantic tools have been developed: semantic wikis are collections of wiki pages that can be annotated with properties and so combine flexibility and expressiveness, two desirable aspects when modeling databases, especially in the dynamic biomedical domain. However, semantics and collaborative analysis of biomedical data is still an unsolved challenge. The …

Epigenetic Resetting In The Human Germ Line Entails Histone Modification Remodeling, Wolfram H Gruhn, Walfred W C Tang, Sabine Dietmann, João P Alves-Lopes, Christopher A Penfold, Frederick C K Wong, Navin B Ramakrishna, M Azim Surani

2020-Current year OA Pubs

Epigenetic resetting in the mammalian germ line entails acute DNA demethylation, which lays the foundation for gametogenesis, totipotency, and embryonic development. We characterize the epigenome of hypomethylated human primordial germ cells (hPGCs) to reveal mechanisms preventing the widespread derepression of genes and transposable elements (TEs). Along with the loss of DNA methylation, we show that hPGCs exhibit a profound reduction of repressive histone modifications resulting in diminished heterochromatic signatures at most genes and TEs and the acquisition of a neutral or paused epigenetic state without transcriptional activation. Efficient maintenance of a heterochromatic state is limited to a subset of genomic …

Differential Effects Of Anti-Cd20 Therapy On Cd4 And Cd8 T Cells And Implication Of Cd20-Expressing Cd8 T Cells In Ms Disease Activity, Koji Shinoda, Anne H Cross, Et Al.

2020-Current year OA Pubs

A small proportion of multiple sclerosis (MS) patients develop new disease activity soon after starting anti-CD20 therapy. This activity does not recur with further dosing, possibly reflecting deeper depletion of CD20-expressing cells with repeat infusions. We assessed cellular immune profiles and their association with transient disease activity following anti-CD20 initiation as a window into relapsing disease biology. Peripheral blood mononuclear cells from independent discovery and validation cohorts of MS patients initiating ocrelizumab were assessed for phenotypic and functional profiles using multiparametric flow cytometry. Pretreatment CD20-expressing T cells, especially CD20

Prm-Reactive Antibodies Reveal A Role For Partially Mature Virions In Dengue Virus Pathogenesis, Kimberly A Dowd, Laura A Vanblargan, Rita E Chen, Bradley M Whitener, Jennifer Govero, Soila Sukupolvi-Petty, Michael S Diamond, Et Al.

2020-Current year OA Pubs

Cleavage of the flavivirus premembrane (prM) structural protein during maturation can be inefficient. The contribution of partially mature flavivirus virions that retain uncleaved prM to pathogenesis during primary infection is unknown. To investigate this question, we characterized the functional properties of newly-generated dengue virus (DENV) prM-reactive monoclonal antibodies (mAbs) in vitro and using a mouse model of DENV disease. Anti-prM mAbs neutralized DENV infection in a virion maturation state-dependent manner. Alanine scanning mutagenesis and cryoelectron microscopy of anti-prM mAbs in complex with immature DENV defined two modes of attachment to a single antigenic site. In vivo, passive transfer of intact …

Conserved Angio-Immune Subtypes Of The Tumor Microenvironment Predict Response To Immune Checkpoint Blockade Therapy, Madhav Subramanian, Ashraf Ul Kabir, Derek Barisas, Karen Krchma, Kyunghee Choi

2020-Current year OA Pubs

Immune checkpoint blockade (ICB) therapy has revolutionized cancer treatment. However, only a fraction of patients respond to ICB therapy. Accurate prediction of patients to likely respond to ICB would maximize the efficacy of ICB therapy. The tumor microenvironment (TME) dictates tumor progression and therapy outcome. Here, we classify the TME by analyzing the transcriptome from 11,069 cancer patients based on angiogenesis and T cell activity. We find three distinct angio-immune TME subtypes conserved across 30 non-hematological cancers. There is a clear inverse relationship between angiogenesis and anti-tumor immunity in TME. Remarkably, patients displaying TME with low angiogenesis with strong anti-tumor …

Posoleucel, An Allogeneic, Off-The-Shelf Multivirus-Specific T-Cell Therapy, For The Treatment Of Refractory Viral Infections In The Post-Hct Setting, Thomas Pfeiffer, Et Al.

2020-Current year OA Pubs

PURPOSE: Viral infections are a major cause of morbidity and mortality following allogeneic hematopoietic cell transplantation (allo-HCT). In the absence of safe and effective antiviral treatments, virus-specific T cells have emerged as a promising therapeutic option. Posoleucel is a multivirus-specific T-cell therapy for off-the-shelf use against six viral infections that commonly occur in allo-HCT recipients: adenovirus, BK virus (BKV), cytomegalovirus, Epstein-Barr virus, human herpes virus-6, and JC virus.

PATIENTS AND METHODS: We conducted an open-label, phase II trial to determine the feasibility and safety of posoleucel in allo-HCT recipients infected with one or more of these viruses. Infections were either …

Entry Receptors - The Gateway To Alphavirus Infection, Ofer Zimmerman, Autumn C. Holmes, Natasha M. Kafai, Lucas J. Adams, Michael S. Diamond

2020-Current year OA Pubs

Alphaviruses are enveloped, insect-transmitted, positive-sense RNA viruses that infect humans and other animals and cause a range of clinical manifestations, including arthritis, musculoskeletal disease, meningitis, encephalitis, and death. Over the past four years, aided by CRISPR/Cas9-based genetic screening approaches, intensive research efforts have focused on identifying entry receptors for alphaviruses to better understand the basis for cellular and species tropism. Herein, we review approaches to alphavirus receptor identification and how these were used for discovery. The identification of new receptors advances our understanding of viral pathogenesis, tropism, and evolution and is expected to contribute to the development of novel strategies …

Glua3 Subunits Are Required For Appropriate Assembly Of Ampar Glua2 And Glua4 Subunits On Cochlear Afferent Synapses And For Presynaptic Ribbon Modiolar-Pillar Morphology, Mark A Rutherford, Atri Bhattacharyya, Maolei Xiao, Hou-Ming Cai, Indra Pal, Maria Eulalia Rubio

2020-Current year OA Pubs

Cochlear sound encoding depends on α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs), but reliance on specific pore-forming subunits is unknown. With 5-week-old male C57BL/6J

Quantification Of Patient-Specific Coronary Material Properties And Their Correlations With Plaque Morphological Characteristics: An In Vivo Ivus Study, Liang Wang, Akiko Maehara, Xiaoguo Zhang, Rui Lv, Yangyang Qu, Xiaoya Guo, Jian Zhu, Zheyang Wu, Kristen L Billiar, Jie Zheng, Lijuan Chen, Genshan Ma, Gary S Mintz, Dalin Tang

2020-Current year OA Pubs

BACKGROUND: A method using in vivo Cine IVUS and VH-IVUS data has been proposed to quantify material properties of coronary plaques. However, correlations between plaque morphological characteristics and mechanical properties have not been studied in vivo.

METHOD: In vivo Cine IVUS and VH-IVUS data were acquired at 32 plaque cross-sections from 19 patients. Six morphological factors were extracted for each plaque. These samples were categorized into healthy vessel, fibrous plaque, lipid-rich plaque and calcified plaque for comparisons. Three-dimensional thin-slice models were constructed using VH-IVUS data to quantify in vivo plaque material properties following a finite element updating approach by matching …

Outcomes Of The 2019 Hydrocephalus Association Workshop, "Driving Common Pathways: Extending Insights From Posthemorrhagic Hydrocephalus", Jason K Karimy, Jessie C Newville, Cameron Sadegh, Jill A Morris, Edwin S Monuki, David D Limbrick, James P Mcallister Ii, Jenna E Koschnitzky, Maria K Lehtinen, Lauren L Jantzie

2020-Current year OA Pubs

The Hydrocephalus Association (HA) workshop, Driving Common Pathways: Extending Insights from Posthemorrhagic Hydrocephalus, was held on November 4 and 5, 2019 at Washington University in St. Louis. The workshop brought together a diverse group of basic, translational, and clinical scientists conducting research on multiple hydrocephalus etiologies with select outside researchers. The main goals of the workshop were to explore areas of potential overlap between hydrocephalus etiologies and identify drug targets that could positively impact various forms of hydrocephalus. This report details the major themes of the workshop and the research presented on three cell types that are targets for new …

Endogenous Retrovirus Rna Expression Differences Between Race, Stage And Hpv Status Offer Improved Prognostication Among Women With Cervical Cancer, Jill Alldredge, Vinay Kumar, James Nguyen, Brooke E Sanders, Karina Gomez, Kay Jayachandran, Jin Zhang, Julie Schwarz, Farah Rahmatpanah

2020-Current year OA Pubs

Endogenous human retroviruses (ERVs) are remnants of exogenous retroviruses that have integrated into the human genome. Using publicly available RNA-seq data from 63 cervical cancer patients, we investigated the expression of ERVs in cervical cancers. Four aspects of cervical cancer were investigated: patient ancestral background, tumor HPV type, tumor stage and patient survival. Between the racial subgroups, 74 ERVs were significantly differentially expressed, with Black Americans having 30 upregulated and 44 downregulated (including MER21C, HERV9-int, and HERVH-int) ERVs when compared to White Americans. We found that 3313 ERVs were differentially expressed between HPV subgroups, including MER41A, HERVH-int and HERVK9. There …

Whole-Exome Sequence Analysis Of Anthropometric Traits Illustrates Challenges In Identifying Effects Of Rare Genetic Variants, Kristin L Young, Ping An, Petra Lenzini, Michael Province, Et Al.

2020-Current year OA Pubs

Anthropometric traits, measuring body size and shape, are highly heritable and significant clinical risk factors for cardiometabolic disorders. These traits have been extensively studied in genome-wide association studies (GWASs), with hundreds of genome-wide significant loci identified. We performed a whole-exome sequence analysis of the genetics of height, body mass index (BMI) and waist/hip ratio (WHR). We meta-analyzed single-variant and gene-based associations of whole-exome sequence variation with height, BMI, and WHR in up to 22,004 individuals, and we assessed replication of our findings in up to 16,418 individuals from 10 independent cohorts from Trans-Omics for Precision Medicine (TOPMed). We identified four …

Long-Term Hematologic Improvement In A Patient With Cytopenic Myelofibrosis Treated With Pacritinib, Abdulraheem Yacoub, Ruben A Mesa, Stephen T Oh

2020-Current year OA Pubs

No abstract provided.

Combining Ivus + Oct Data, Biomechanical Models And Machine Learning Method For Accurate Coronary Plaque Morphology Quantification And Cap Thickness And Stress/Strain Index Predictions, Rui Lv, Liang Wang, Akiko Maehara, Mitsuaki Matsumura, Xiaoya Guo, Habib Samady, Don P Giddens, Jie Zheng, Gary S Mintz, Dalin Tang

2020-Current year OA Pubs

Assessment and prediction of vulnerable plaque progression and rupture risk are of utmost importance for diagnosis, management and treatment of cardiovascular diseases and possible prevention of acute cardiovascular events such as heart attack and stroke. However, accurate assessment of plaque vulnerability assessment and prediction of its future changes require accurate plaque cap thickness, tissue component and structure quantifications and mechanical stress/strain calculations. Multi-modality intravascular ultrasound (IVUS), optical coherence tomography (OCT) and angiography image data with follow-up were acquired from ten patients to obtain accurate and reliable plaque morphology for model construction. Three-dimensional thin-slice finite element models were constructed for 228 …

Students Stress Patterns In A Kenyan Socio-Cultural And Economic Context: Toward A Public Health Intervention, Victoria N Mutiso, David M Ndetei, Esther N Muia, Christine Musyimi, Monicah Masake, Tom L Osborn, Andre Sourander, John R Weisz, Daniel Mamah

2020-Current year OA Pubs

This study aimed at determining the prevalence of stress, different types of stress, their severity and their determinants in Kenyan university, college and high school students. The following tools were administered to 9741 students: (1) Researcher-designed socio-demographic tool, (2) Psychiatric Diagnostic Screening Questionnaire (PDSQ) for psychiatric disorders, (3) WERC Stress Screen for stress, (4) Washington Early Recognition Center Affectivity and Psychosis (WERCAP) screen for psychosis and affectivity, (5) Wealth Index Questionnaire for economic indicators. Descriptive analysis for the prevalence of different types of stress and inferential analysis for stress and independent variables were done. Significant variables (p < 0.05) were fitted into generalized linear model to determine independent predictors. The mean age of the respondents was 21.4 years (range 16-43). Money issues were the commonest stressors while alcohol and drug use were the least. The independent predictors of stress were females, college students and use of gas stove. In conclusion, up to 30% of the students suffer from mild to severe stress. The students experience a wide range of stressors. The most important stressors include money and finances, family related problems and concerns about their future. Our findings suggest a public health approach to create stress awareness in students.

Postibrutinib Relapse Outcomes For Patients With Marginal Zone Lymphoma, Narendranath Epperla, Lauren Shea, Nancy L Bartlett, Et Al.

2020-Current year OA Pubs

No abstract provided.

Gene Regulatory Network Reconfiguration In Direct Lineage Reprogramming, Kenji Kamimoto, Mohd Tayyab Adil, Kunal Jindal, Christy M Hoffmann, Wenjun Kong, Xue Yang, Samantha A Morris

2020-Current year OA Pubs

In direct lineage conversion, transcription factor (TF) overexpression reconfigures gene regulatory networks (GRNs) to reprogram cell identity. We previously developed CellOracle, a computational method to infer GRNs from single-cell transcriptome and epigenome data. Using inferred GRNs, CellOracle simulates gene expression changes in response to TF perturbation, enabling in silico interrogation of network reconfiguration. Here, we combine CellOracle analysis with lineage tracing of fibroblast to induced endoderm progenitor (iEP) conversion, a prototypical direct reprogramming paradigm. By linking early network state to reprogramming outcome, we reveal distinct network configurations underlying successful and failed fate conversion. Via in silico simulation of TF perturbation, …

Discussing Cost And Value In Patient Decision Aids And Shared Decision Making: A Call To Action, Mary C. Politi, Ashley J. Housten, Rachel C. Forcino, Jesse Jansen, Glyn Elwyn

2020-Current year OA Pubs

Direct and indirect costs of care influence patients' health choices and the ability to implement those choices. Despite the significant impact of care costs on patients' health and daily lives, patient decision aid (PtDA) and shared decision-making (SDM) guidelines almost never mention a discussion of costs of treatment options as part of minimum standards or quality criteria. Given the growing study of the impact of costs in health decisions and the rising costs of care more broadly, in fall 2021 we organized a symposium at the Society for Medical Decision Making's annual meeting. The focus was on the role of …

Childhood Adversities And Risk Of Posttraumatic Stress Disorder And Major Depression Following A Motor Vehicle Collision In Adulthood, H N Ziobrowski, S L House, D M Barch, Et Al.

2020-Current year OA Pubs

AIMS: Childhood adversities (CAs) predict heightened risks of posttraumatic stress disorder (PTSD) and major depressive episode (MDE) among people exposed to adult traumatic events. Identifying which CAs put individuals at greatest risk for these adverse posttraumatic neuropsychiatric sequelae (APNS) is important for targeting prevention interventions.

METHODS: Data came from

RESULTS: Most participants (90.9%) reported at least rarely having experienced some CA. Ever experiencing each CA other than emotional neglect was univariably associated with 3-month APNS (RRs = 1.31-1.60). Each CA frequency was also univariably associated with 3-month APNS (RRs = 1.65-2.45). In multivariable models, joint associations of CAs with 3-month …

Dry Eye Disease In Mice Activates Adaptive Corneal Epithelial Regeneration Distinct From Constitutive Renewal In Homeostasis, Joseph B Lin, Xiaolei Shen, Charles W Pfeifer, Fion Shiau, Andrea Santeford, Philip A Ruzycki, Brian S Clark, Qin Liu, Andrew J W Huang, Rajendra S Apte

2020-Current year OA Pubs

Many epithelial compartments undergo constitutive renewal in homeostasis but activate unique regenerative responses following injury. The clear corneal epithelium is crucial for vision and is renewed from limbal stem cells (LSCs). Using single-cell RNA sequencing, we profiled the mouse corneal epithelium in homeostasis, aging, diabetes, and dry eye disease (DED), where tear deficiency predisposes the cornea to recurrent injury. In homeostasis, we capture the transcriptional states that accomplish continuous tissue turnover. We leverage our dataset to identify candidate genes and gene networks that characterize key stages across homeostatic renewal, including markers for LSCs. In aging and diabetes, there were only …

A Cdh3-Β-Catenin-Laminin Signaling Axis In A Subset Of Breast Tumor Leader Cells Control Leader Cell Polarization And Directional Collective Migration, Priscilla Y Hwang, Jairaj Mathur, Yanyang Cao, Jose Almeida, Jiayu Ye, Vasilios Morikis, Daphne Cornish, Maria Clarke, Sheila A Stewart, Amit Pathak, Gregory D Longmore

2020-Current year OA Pubs

Carcinoma dissemination can occur when heterogeneous tumor and tumor-stromal cell clusters migrate together via collective migration. Cells at the front lead and direct collective migration, yet how these leader cells form and direct migration are not fully appreciated. From live videos of primary mouse and human breast tumor organoids in a 3D microfluidic system mimicking native breast tumor microenvironment, we developed 3D computational models, which hypothesize that leader cells need to generate high protrusive forces and overcome extracellular matrix (ECM) resistance at the leading edge. From single-cell sequencing analyses, we find that leader cells are heterogeneous and identify and isolate …

Histopathologic And Proteogenomic Heterogeneity Reveals Features Of Clear Cell Renal Cell Carcinoma Aggressiveness, Yize Li, Yige Wu, Rita Jiu-Hsien Lu, Siqi Chen, Yanyan Zhao, Wagma Caravan, Nataly Naser Al Deen, Matthew A Wyczalkowski, Michael C Wendl, Melissa A Reimers, Russell Pachynski, Brian A Van Tine, Feng Chen, Li Ding, Et Al.

2020-Current year OA Pubs

Clear cell renal cell carcinomas (ccRCCs) represent ∼75% of RCC cases and account for most RCC-associated deaths. Inter- and intratumoral heterogeneity (ITH) results in varying prognosis and treatment outcomes. To obtain the most comprehensive profile of ccRCC, we perform integrative histopathologic, proteogenomic, and metabolomic analyses on 305 ccRCC tumor segments and 166 paired adjacent normal tissues from 213 cases. Combining histologic and molecular profiles reveals ITH in 90% of ccRCCs, with 50% demonstrating immune signature heterogeneity. High tumor grade, along with BAP1 mutation, genome instability, increased hypermethylation, and a specific protein glycosylation signature define a high-risk disease subset, where UCHL1 …

Docosahexaenoic Acid Stability In Ready-To-Use Therapeutic Food, Genevieve James, Kevin Stephenson, Meghan Callaghan-Gillespie, Mohamed Tabita Kamara, Hui Gyu Park, J Thomas Brenna, Mark J Manary

2020-Current year OA Pubs

Ready-to-use therapeutic food (RUTF) is used to treat young children diagnosed with severe acute malnutrition. RUTF with low and balanced linoleic and alpha-linolenic acid, plus omega-3 docosahexaenoic acid (DHA), supports long-term cognitive recovery. DHA is prone to degradation due to peroxidation, possibly exacerbated by the iron inherently in RUTF. Our goals were to prepare benchtop and manufacturing scale of RUTF formulations that include DHA and measure its retention. Twenty-seven RUTF formulas with base ingredients, including oats, high oleic or commodity peanuts, and encapsulated or oil-based DHA at various levels were prepared at benchtop scale, followed by seven months of climate-controlled …

Gene-Folic Acid Interactions And Risk Of Conotruncal Heart Defects: Results From The National Birth Defects Prevention Study, Daniel M Webber, Ming Li, Stewart L Macleod, Xinyu Tang, Joseph W Levy, Mohammad A Karim, Stephen W Erickson, Charlotte A Hobbs, The National Birth Defects Prevention Study

2020-Current year OA Pubs

Conotruncal heart defects (CTDs) are heart malformations that affect the cardiac outflow tract and typically cause significant morbidity and mortality. Evidence from epidemiological studies suggests that maternal folate intake is associated with a reduced risk of heart defects, including CTD. However, it is unclear if folate-related gene variants and maternal folate intake have an interactive effect on the risk of CTDs. In this study, we performed targeted sequencing of folate-related genes on DNA from 436 case families with CTDs who are enrolled in the National Birth Defects Prevention Study and then tested for common and rare variants associated with CTD. …

Fungal Assisted Valorisation Of Polymeric Lignin: Mechanism, Enzymes And Perspectives, Kavya Dashora, Meghana Gattupalli, Gyan Datta Tripathi, Zoya Javed, Shweta Singh, Maria Tuohy, Prakash Kumar Sarangi, Deepti Diwan, Harikesh B. Singh, Vijai Kumar Gupta

2020-Current year OA Pubs

No abstract provided.

Relations Of Current And Past Cancer With Severe Outcomes Among 104,590 Hospitalized Covid-19 Patients: The Covid Ehr Cohort At The University Of Wisconsin, Margaret B Nolan, Li-Shiun Chen, Et Al.

2020-Current year OA Pubs

BACKGROUND: There is mixed evidence about the relations of current versus past cancer with severe COVID-19 outcomes and how they vary by patient and cancer characteristics.

METHODS: Electronic health record data of 104,590 adult hospitalized patients with COVID-19 were obtained from 21 United States health systems from February 2020 through September 2021. In-hospital mortality and ICU admission were predicted from current and past cancer diagnoses. Moderation by patient characteristics, vaccination status, cancer type, and year of the pandemic was examined.

RESULTS: 6.8% of the patients had current (n = 7,141) and 6.5% had past (n = 6,749) cancer diagnoses. Current …

Discovery And Characterization Of A New Class Of O-Linking Oligosaccharyltransferases From The Moraxellaceae Family, Cory J Knoot, Paeton L Wantuch, Lloyd S Robinson, David A Rosen, Nichollas E Scott, Christian M Harding

2020-Current year OA Pubs

Bacterial protein glycosylation is commonly mediated by oligosaccharyltransferases (OTases) that transfer oligosaccharides en bloc from preassembled lipid-linked precursors to acceptor proteins. Natively, O-linking OTases usually transfer a single repeat unit of the O-antigen or capsular polysaccharide to the side chains of serine or threonine on acceptor proteins. Three major families of bacterial O-linking OTases have been described: PglL, PglS, and TfpO. TfpO is limited to transferring short oligosaccharides both in its native context and when heterologously expressed in glycoengineered Escherichia coli. On the other hand, PglL and PglS can transfer long-chain polysaccharides when expressed in glycoengineered E. coli. Herein, we …

Silencing Mir-146a-5p Protects Against Injury-Induced Osteoarthritis In Mice, Haocheng Qin, Cuicui Wang, Yonghua He, Aiwu Lu, Tiandao Li, Bo Zhang, Jie Shen

2020-Current year OA Pubs

Osteoarthritis (OA), the most prevalent joint disease and the leading cause of disability, remains an incurable disease largely because the etiology and pathogenesis underlying this degenerative process are poorly understood. Low-grade inflammation within joints is a well-established factor that disturbs joint homeostasis and leads to an imbalance between anabolic and catabolic processes in articular cartilage; however, the complexity of the network between inflammatory factors that often involves positive and negative feedback loops makes current anti-cytokine therapy ineffective. MicroRNAs (miRNAs) have emerged as key regulators to control inflammation, and aberrant miRNAs expression has recently been linked to OA pathophysiology. In the …

Use Of Serial Smartphone-Based Assessments To Characterize Diverse Neuropsychiatric Symptom Trajectories In A Large Trauma Survivor Cohort, Francesca L Beaudoin, Stacey L House, Deanna M Barch, Et Al.

2020-Current year OA Pubs

The authors sought to characterize adverse posttraumatic neuropsychiatric sequelae (APNS) symptom trajectories across ten symptom domains (pain, depression, sleep, nightmares, avoidance, re-experiencing, anxiety, hyperarousal, somatic, and mental/fatigue symptoms) in a large, diverse, understudied sample of motor vehicle collision (MVC) survivors. More than two thousand MVC survivors were enrolled in the emergency department (ED) and completed a rotating battery of brief smartphone-based surveys over a 2-month period. Measurement models developed from survey item responses were used in latent growth curve/mixture modeling to characterize homogeneous symptom trajectories. Associations between individual trajectories and pre-trauma and peritraumatic characteristics and traditional outcomes were compared, along …