Digital Commons Network^™

Il-10 And Tgf-Beta Increase Connexin-43 Expression And Membrane Potential Of Hl-1 Cardiomyocytes Coupled With Raw 264.7 Macrophages, Cora B. Cox

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Cardiomyocytes and macrophages have been found to interact via connexin-43 hemichannels. The role of connexin-43, however, is not fully understood. This study shows that these interactions aid in increasing the membrane potential of cardiomyocytes allowing contraction of the cells. HL-1 cardiomyocytes and RAW 264.7 macrophages in coculture increased expression of connexin-43 compared to cardiomyocytes alone. Co-cultures also increased the fluorescence of Di-8-ANEPPS potentiometric dye indicating an increase in cardiomyocyte membrane potential. Treatment with IL-10 and TGF-beta further increased connexin-43 expression and membrane potential. Treatment with SOCS3 inhibited the effects of TGF-beta and IL-10 while having no effect on its own. …

Il-10 And Tgf-Beta Increase Connexin-43 Expression And Membrane Potential Of Hl-1 Cardiomyocytes Coupled With Raw 264.7 Macrophages, Cora B. Cox

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Cardiomyocytes and macrophages have been found to interact via connexin-43 hemichannels. The role of connexin-43, however, is not fully understood. This study shows that these interactions aid in increasing the membrane potential of cardiomyocytes allowing contraction of the cells. HL-1 cardiomyocytes and RAW 264.7 macrophages in coculture increased expression of connexin-43 compared to cardiomyocytes alone. Co-cultures also increased the fluorescence of Di-8-ANEPPS potentiometric dye indicating an increase in cardiomyocyte membrane potential. Treatment with IL-10 and TGF-beta further increased connexin-43 expression and membrane potential. Treatment with SOCS3 inhibited the effects of TGF-beta and IL-10 while having no effect on its own. …

Investigating Streptococcus Pneumoniae And Adenovirus Co-Infections Of Lung Epithelial Cells, Mark Nicholas Calabro

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Co-infection is common among viruses and bacteria in the human respiratory system. Adenovirus (AdV) and Streptococcus pneumoniae are clinically relevant respiratory pathogens that cause morbidity and mortality in a variety of patient populations with the highest morbidity occurring among immunocompromised individuals, but also prevalent in infants and the elderly. Acute respiratory distress syndrome may become severe in healthy individuals when co-infection with S. pneumoniae and AdV occurs due to synergistic effects of the pathogens on the host. I hypothesized that S. pneumoniae infection decreases AdV transduction of airway epithelia. To test this hypothesis, we utilized the polarized immortalized airway epithelial …

Investigating Streptococcus Pneumoniae And Adenovirus Co-Infections Of Lung Epithelial Cells, Mark Nicholas Calabro

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Co-infection is common among viruses and bacteria in the human respiratory system. Adenovirus (AdV) and Streptococcus pneumoniae are clinically relevant respiratory pathogens that cause morbidity and mortality in a variety of patient populations with the highest morbidity occurring among immunocompromised individuals, but also prevalent in infants and the elderly. Acute respiratory distress syndrome may become severe in healthy individuals when co-infection with S. pneumoniae and AdV occurs due to synergistic effects of the pathogens on the host. I hypothesized that S. pneumoniae infection decreases AdV transduction of airway epithelia. To test this hypothesis, we utilized the polarized immortalized airway epithelial …

Capparis Decidua Edgew (Forssk.) Stem Extract Alleviates The Water Stress Perturbations In Wheat (Triticum Aestivum L.) At Early Growth Stage, Muhammad Nawaz, Qasim Ali, Muhammad Azeem, Fehmina Asghar, Hasnain Ishaq, Naeem Iqbal, Sajid -Ur-Rahman

Journal of Bioresource Management

Water shortage is one of the major limiting factors of crop productivity worldwide. This study was planned to explore the impact of Capparis decidua stem extract on wheat varieties (FSD-2008, 12066 and 13348) under water stress condition. Water deficit condition negatively impacted the seedling germination, growth, photosynthetic pigments and ions uptake capacity. Water limited condition significantly (p≤0.05) increased time to attain 50% germination, lipid peroxidation, H₂O₂ and enhanced the antioxidant mechanism to overcome the oxidative stress. Different concentrations of C. decidua extract i.e. Not Soaking (NS), 10%, 20%, 30% and 40% were used for seed …

Quantitative Analysis Of Phytochemical Compounds In Barks And Leaves Of Okoubaka Aubrevillei Collected From Iwo, Southwestern Nigeria, Oluwatoyin Opeyemi Akintola, Adeboyin Funmi Aderounmu, Isaac Oluwaseyi Abiola, Kolawole Emmanuel Abodurin, Tunde Adeniran, Festus Agboola, Oluwayemisi Samuel Olokeogun

Journal of Bioresource Management

Okoubaka aubrevillei is an indigenous and sacred tropical tree in Africa. It is rare with allelopathic properties and has relatively little information available in terms of what is responsible for its usage for medicinal and traditional usage. The phytochemical screening and quantitative analysis of the compounds in the barks and leaves of Okoubaka aubrevillei was determined to ascertain and establish its earlier claimed usage as traditional and modern medicine. The most important classes of secondary metabolites (phytochemical compounds) specifically alkaloids, flavonoids, tannins, saponins, anthraquinnes, mucilages, oses, holosides, coumarin and glycosides were detected in both, the leaves and barks of the …

Prevalence Of Tuberculosis In District Haveli, Azad Jammu And Kashmir, Abu Ul Hassan Faiz, Ghazal Tariq, Ayesha Faiz, Lariab Zahra Faiz

Journal of Bioresource Management

Tuberculosis is an infectious disease with a common occurrence in population of Azad Jammu and Kashmir. The current study was designed to find out the prevalence of TB among the general population of a less developed District Haveli. Samples were collected from different hospitals and TB centers located in district Haveli and tested through sputum smear microscopy for conformation of positive for TB. The three-year (2015 to 2018) study was conducted to record prevalence of tuberculosis in selected area of Azad Jammu and Kashmir. The trend line of prevalence was made by using (SPSS software). Results produced linear trends yearly …

The Guardian, Week Of September 14, 2020, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the week of September 14, 2020. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.

Human Botfly Infestation: Furuncular Myiasis, Rachel Rhee, Christina E. Borchers, Samia Borchers, Gregory Maupin, Jeffery Weinstein

Dermatology Faculty Publications

Dermatobia hominis is one of many species of botflies whose larvae is parasitic to humans and is uniquely indigenous to Central and South America. The larvae of the human botfly gain nutrients to survive by feeding underneath mammal skin. As a consequence, within 24 hours, small 2- 3mm painful, erythematous, draining, and purulent papules with a classical air-pore develop as the initial cutaneous sign. At first, the lesions resemble that of an insect bite, but over time they enlarge to 10-35mm in diameter with a surrounding area of induration. The most common areas where the larvae are cutaneously deposited include …

Current Scenario Of Covid-19 With Epidemiological And Phylogenetic Analysis Of Pakistani Coronavirus: A Review, Asma Altaf, Wafa Iqbal, Sidra Younis, Farah Deeba

Journal of Bioresource Management

A novel corona virus named as 2019 n-CoV was identified to be the actual cause of an outbreak of respiratory illness in Wuhan, China beginning in late December 2019. This respiratory disease was named as COVID-19 by WHO. There are many countries affected by COVID-19 including Pakistan. In this review we have provided a brief introduction of COVID-19 and discussed epidemiological and phylogenetic analysis of Pakistani novel corona virus strain. Our epidemiological analysis of data till 16^th May 2020 showed rapidly increasing number of new cases, while at the same time a higher recovery rate than number of deaths …

The Guardian, Week Of September 7, 2020, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the week of September 7, 2020. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.

The Guardian, Week Of August 24, 2020, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the week of August 24, 2020. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.

Provost Newsletter - August 14, 2020, Office Of The Provost, Wright State University

Office of the Provost Newsletters and Announcements

The Newsletter from the Office of the Provost regarding specifics about the plans and process in place for the 2020 Fall Semester.

Seroprevalence, Biochemical Investigation And Risk Factor Assessment For Hbv & Hcv Infection In Hospital Based Patients Of Islamabad, Pakistan, Narmeen Adnan Rana, Bushra Munir, Nazeer Hussain, Nazia Imtiaz, Muhammad Asif Gondal Dr., Fahed Parvaiz Dr.

Journal of Bioresource Management

Viral hepatitis poses a serious threat to mankind. Hepatitis B and C Virus are blood borne pathogens that affect millions of individually globally. This study was conducted on a hospital-based population in Islamabad, Pakistan over a period of 4 months, utilizing ELISA as the diagnostic technique which suggested a higher seroprevalence rate for both HBV and HCV i.e. 2.07% and 8.24% respectively. A correlational analysis of the biochemical parameters of these individuals with HBV and HCV infection was carried out and the results indicated a positive correlation of HBV with Alkaline Phosphatase (ALP), HCV with Total Bilirubin (TBil) and both …

The Psychology Of Trauma And Covid-19, Jeremiah Schumm, Craig Woolley, Laura M. Luehrmann, Cheryl L. Meyer

School of Professional Psychology Faculty Publications

This is the third installment in the Shelter in Place (SiP) Lecture series. This installment deals with the impact and implications of the Shelter in Place order in terms of Psychological Trauma.

Wsu Research News, Spring 2020, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A thirty-two page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.

Anthrax Event Detection Using Twitter: Analysis Of Unigram And Bigrams For Relevant Vs Non-Relevant Tweets, Michele Miller, William L. Romine

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Materials

Due to the lack of anthrax attacks in recent times, researchers have used naturally occurring events to assess their anthrax detection models, but these provide little information on how the models will perform in the context of an unannounced, intentional release of a bioterrorism agent, like anthrax. Therefore, it is important to develop a detection model using data surrounding real anthrax scares and events.We develop a methodology to detect an anthrax-related event on Twitter. We describe a process to separate the tweets concerning anthrax-related events from those not related so experts can address misconceptions and fears in real-time.Most tweets were …

Enhanced Expression Of Receptor Tyrosine Kinase Mer (Mertk) On Socs3-Treated Polarized Raw 264.7 Anti-Inflammatory M2c Macrophages, Sankhadip Bhadra

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Materials

Macrophages are phagocytic cells located in tissues, organs and even circulated within our body as white blood cells. Based on the local cytokine milieu in tissue sites, macrophages may be polarized into pro-inflammatory M1 or anti-inflammatory M2 phenotypes. Receptor tyrosine kinase Mer (MERTK) helps in clearing dead neutrophils and other apoptotic cells from damaged tissue sites preventing chronic inflammation and autoimmune disorders. MERTK aids in the maintenance of tissue homeostasis and wound healing. Phosphatidylserine (PtdSer) present on the surface of apoptotic cells release “eat me” signals which are recognized by the two “bridging ligands” of MERTK receptor, Gas6 and ProS. …

Provost Newsletter - March 9, 2020, Office Of The Provost, Wright State University

Office of the Provost Newsletters and Announcements

The Newsletter from the Office of the Provost with a message from Interim Provost Leaman addressing some COVID-19 concerns as well as the University's new travel guidelines.

The Guardian, Week Of March 9, 2020, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the week of March 9, 2020. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.

The Guardian, Week Of February 3, 2020, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the week of January 6, 2020. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.

Mandatory And Permissive Reporting Laws: Obligations, Challenges, Moral Dilemmas, And Opportunities, Joel M. Geiderman, Catherine A. Marco

Emergency Medicine Faculty Publications

The duty to report certain conditions to public health or law enforcement authorities is one that falls on all physicians and other health care workers as part of their duty to protect the public from harm. In an open society, others, such as teachers, clergy, police officers, or simply neighbors, share the responsibility of protecting individuals at risk, often by reporting them to authorities. The emergency physician and others in the emergency department are uniquely positioned to identify people at risk or who pose a risk, and to report them as required or allowed under the law. In some circumstances, …

Potential Role Of Ahr In Antibody Production, Mili Bhakta

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Aryl hydrocarbon receptor (AhR) mediates the immunosuppressive effects of 2,3,7,8 -tetrachlorodibenzodioxin (TCDD) in murine B cells. The effects of AhR activation on the regulation of expression of human immunoglobulin isotypes (μ, γ1-4, α1-2 and ε) and Ig secretion is unclear. Our previous results using CL-01 cell-line originating from a Burkitt’s lymphoma patient, demonstrated an inhibitory effect of TCDD on IgG expression but a surprising and marked loss of IgG secretion when the AhR was knocked out by siRNA or CRISPR/Cas9 gene editing. To determine if the AhR is a critical mediator of IgG expression, current study is focused on characterizing …

The Effects Of Foodborne Bacteria On Outbreaks, Illnesses, And Hospitalizations In The U.S., Henrietta Ofori-Sampong

Scholarship in Medicine - All Papers

Objective: To investigate the effects of foodborne bacteria on outbreaks, and outbreak-associated illnesses and hospitalizations in the United States (U.S.).

Methods: Aggregate data was obtained from the CDC WONDER (Wide-ranging Online Data for Epidemiologic Research) database showing the number of foodborne outbreaks (1993-2017), and outbreak-associated illnesses (1993-2017) and hospitalizations (1998-2017) caused by foodborne bacteria. Species of bacteria were grouped into 13 genera and 1 other. An Analysis of variance (ANOVA) test was used to compare the mean outbreaks, and outbreak-associated illnesses and hospitalizations for each of the years using the data by species for each year. Results: There was no …

A Discrete Age Structured Model Of Hantavirus In A Rodent Reservoir In Paraguay, Megan A. Rúa

Biological Sciences Faculty Publications

Many rodent-borne hantaviruses are zoonotic pathogens that can cause disease in humans through inhalation of aerosolized rodent excreta. To evaluate the prevalence of Jaborâ virus (JABV) over time within its rodent reservoir, Akodon montensis, we formulated a mathematical model with multiple rodent age classes and unique infection class progression features. We then parameterized the model with data collected from a survey of JABV harbored by Akodon montensis in the Mbaracayú Reserve in Paraguay. Our model incorporates three types of infection over the lifetime of the rodent as well as a recovered class. A new feature of the model allows transition …

Impact Of Different Factors On Premature Deaths In Rural And Urban Virginian Counties, Christian Randazzo

Scholarship in Medicine - All Papers

The goal of this investigation is to analyze data that influences premature death rates in Virginian counties. Specifically, I am looking at how the rates compare between rural and urban Virginian counties in 2020. In addition, I am analyzing how the premature death rates correlates with low birthweights, with air pollution, with preventable hospital stays, and with children in poverty and see how these variables predict the variance in premature death rates by county. I am using data from the County Health Rankings website. They gathered their data via surveys, the National Vital Statistics System, the CDC health tracking network, …

The Persisting Threats Of Cholera: A Cyclical Public Health Problem In Ghana, Rita Laryea Amediavor

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The prevalence of communicable diseases continues to be one of the continent's leading causes of deaths. Cholera is a waterborne disease triggered by toxigenic strains of the Gram-negative bacteria Vibrio Cholerae O1 strain and less common O139 strain. with symptoms such as severe acute watery diarrhea and vomiting leading to dehydration, progressing to hypovolemic shock and death if not treated timely due to its short incubation period (Pasetto et al., 2018). West African countries are largely portrayed as endemic to cholera, though the dynamics of outbreaks in these developing countries remain largely uncertain. The purpose of the study is to …

Anthrax Event Detection: Analysis Of Public Opinion Using Twitter During Anthrax Scares, The Mueller Investigation, And North Korean Threats, Michele E. Miller

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When people allow fear to drive their decision making, they often make decisions that do more harm than good. Examples of this include stocking up on ciprofloxacin, flooding doctors’ offices and buying black market antibiotics after the anthrax attacks of 2001. Therefore, it is important to be able to address what people are saying when another anthrax attack occurs. Supervised and unsupervised machine learning methodologies can be utilized to detect an event, classify the tweets by event, and to determine the main topics of discussion. Over the period of data collection, twenty events were detected. Three of these events concerned …

Host-Parasite Interactions: Comparative Analyses Of Population Genomics, Disease-Associated Genomic Regions, And Host Use, Sara Rose Seibert

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Birds are major vectors and reservoir hosts of pathogens across our globe. Pathogens can be spread through the dispersal and seasonal movements of migratory and resident wild birds. Although biogeographic barriers have the potential to restrict completely or in part gene flow in wild birds, variation in ecological drivers of dispersal likely influences the effectiveness of these barriers amongst different species. This dissertation seeks to enhance our knowledge of Southern Hemisphere waterfowl species’ dispersal, population genomics, and disease ecology using population genomic techniques (double-digest restriction-site associated DNA sequencing (ddRADseq)) and multi-variate statistics. First, we investigated the roles of dispersal behavior …

Vital Signs, Fall 2019, Boonshoft School Of Medicine

Vital Signs

A thirty-two page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles, alumni profiles, class notes, and more.