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A Novel Molecular Relationship Between Parn And Pld That, When Deregulated, Contributes To The Aggressive Phenotype Of Breast Cancer Cell Lines., Taylor Elaine Miller

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The removal of mRNA transcript poly(A) tails by 3'-5' exonucleases is the rate-limiting step for controlled mRNA decay in eukaryotes. Poly(A)-specific ribonuclease (PARN) is one such exonuclease that degrades poly(A) tails, and although its in vitro activity is well-characterized, PARN’s patho-physiological roles in the cell are not well understood. Prior studies have found a possible role for PARN in cancer, in that PARN expression levels in human breast cancer tissues are often decreased compared to normal control tissues. Indeed, data mined from the ONCOMINE cancer array database showed that PARN is downregulated in patient invasive breast carcinoma samples compared to …

Phospholipase D: Key Player In Macrophage-Mediated Inflammation And Resolution, Ramya Ganesan

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Phospholipase D (PLD) is a cell membrane remodeling and signaling protein implicated in the pathology of chronic inflammation. As PLD is also central to macrophage cell migration, we investigated the molecular basis of PLD's involvement and regulation in macrophage-initiated inflammation (atherosclerosis) and resolution. We have found that PLD is associated with signaling proteins and positively affects cell movement, phagocytosis and NADPH-initiated release of Reactive Oxygen Species (ROS). We found that PLD2 but not PLD1 is important for foam cell formation that causes atherogenesis. We have also found a novel way of inducing macrophage class-switch (polarization) by PLD overexpression. PLD induces …

Proteomic Analysis Of Ischemic Stroke Blood Biomarkers, April Arnold Daubenspeck

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Stroke is a global burden that claims 6 million lives and permanently disables another 5 million each year. The risk of morbidity and mortality following stroke decreases when early diagnosis and treatment are achieved. However there is no blood test to diagnose ischemic stroke because biomarkers to date have not shown adequate sensitivity and specificity to be adopted into hospital protocols. The overall objective of this dissertation was to identify novel blood protein biomarkers of ischemic stroke. We achieved this by using a number of proteomic and statistical techniques to investigate the differential abundance of proteins in the blood of …

Association Of Gut Luminal Metabolites And Allergic Responses, Ghaith Mohammed Fallata

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Allergy is a global health problem, affecting more than 40% of the world population. Allergy is often associated with a dysregulation of the immune system that becomes overactive against allergens. Several genes are known to be important for the proper homeostasis of the immune system in the epithelial tissues, and the loss of function of genes such as IKKß or SOX9 impacts the interaction between external stimuli and the host. Many of the allergic responses are triggered through the regulatory cascade governed by transcriptional factor NF-κB. Regulation of NF-κB activity is complex, involves several alternative routes, and is also influenced …

Central Research Forum Program - 2016, Office Of Research Affairs, Boonshoft School Of Medicine

Central Research Forum

The 22-page program includes a schedule, speaker biographies, and presentation titles from the 2016 Central Research Forum held by the Boonshoft School of Medicine's Office of Research Affairs.

Flight Physician - October, 2016, Civil Aviation Medical Association

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A twenty-seven page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Undergraduate And Graduate Course Descriptions, 2016 Fall, Wright State University

Course Descriptions

Wright State University undergraduate and graduate course descriptions from Fall 2016.

Flight Physician - July, 2016, Civil Aviation Medical Association

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A twenty-one page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

What Motivates High School Students To Take Precautions Against The Spread Of Influenza? A Data Science Approach To Latent Modeling Of Compliance With Preventative Practice, William L. Romine, Tanvi Banerjee, William R. Folk, Lloyd H. Barrow

Kno.e.sis Publications

– This study focuses on a central question: What key behavioral factors influence high school students’ compliance with preventative measures against the transmission of influenza? We use multilevel logistic regression to equate logit measures for eight precautions to students’ latent compliance levels on a common scale. Using linear regression, we explore the efficacy of knowledge of influenza, affective perceptions about influenza and its prevention, prior illness, and gender in predicting compliance. Hand washing and respiratory etiquette are the easiest precautions for students, and hand sanitizer use and keeping the hands away from the face are the most difficult. Perceptions of …

Undergraduate And Graduate Course Descriptions, 2016 Summer, Wright State University

Course Descriptions

Wright State University undergraduate and graduate course descriptions from Summer 2016.

Department Of Surgery Update, June 2016, Wright State University Department Of Surgery

Department of Surgery Updates

A six page newsletter created by the Department of Surgery documenting changes to the department. This issue contains a message from the chair, a list of important dates, committee meetings, scholarly activity, and more.

Microwave Assisted Extraction Of Phenolics From Trachyspermum Ammi (L.) Sprague, Sumera Javad, Maimoona Mubarik, Arusa Aftab, Amina Tariq

Journal of Bioresource Management

Trachyspermum ammi (L.) Sprague is an annual herbaceous plant belonging to the family Apiaceae and is mostly administered for stomach disorders. During the present research work, cold maceration and Microwave Assisted Extraction (MAE) of T. ammi Sprague was done by using methanol, dichloromethane and n-hexane separately as extracting solvents. For cold maceration, 2, 4, 6, 18 and 24 hours of time durations were used for each solvent. Maximum extract (50mg/g) was obtained at 18 hours of cold maceration with methanol, while maximum phenolics were also obtained from the same extract, which were calculated to be 170.89μg/g equivalent of Gallic acid. …

Management Of Sclerotium Rolfssi Through Methanolic Leaf Extract Of Alstonia Scholaris (L.) R. Br. And Azadirachta Indica L., Beenish Zia Butt, Nighat Sana, Saba Butt

Journal of Bioresource Management

Sclerotium rolfsii is a polyphagous soil borne fungus infecting more than 500 plant species across the world that is causing vast losses. Although the fungus is soil and seed borne, soil borne inoculum is most vital in infection-causing and syndrome development. Treating soil borne pathogens with fungicides is not reasonable due to very high costs. Environmental hazards are also involved. Therefore, integrated management of pathogens using biological controlling agents is the paramount alternative. Extracts of higher plants have demonstrated a wide range of activity against plant pathogenic organisms. The present research work was carried out to manage the pathogen and …

Germline Defects Caused By Smed-Boule Rna-Interference Reveal That Egg Capsule Deposition Occurs Independently Of Fertilization, Ovulation, Mating, Or The Presence Of Gametes In Planarian Flatworms, Jessica Kathryne Steiner, Junichi Tasaki, Labib Rouhana

Biological Sciences Faculty Publications

Few animals are known to lay eggs in the absence of ovulation or copulation, as it is presumably energetically wasteful and subjected to negative selection. Characterization of Smed-boule, a member of the DAZ family of germline RNA-binding proteins, revealed that egg capsule (or capsule) production and deposition occurs independently of the presence of gametes in the planarian flatworm Schmidtea mediterranea. Reduction of Smed-boule expression by RNA-interference (RNAi) causes ablation of spermatogonial stem cells and the inability of ovarian germline stem cells to undergo oogenesis. Although animals subjected to Smed-boule RNAi lose their gametes and become sterile, they continue …

Ochratoxicosis In Monograstric Animals - A Review, Nafeesa Qudsia Hanif

Journal of Bioresource Management

Ochratoxin A (OTA) is a mycotoxin produced by several fungi of the genera Aspergillus and Penicillium, principally P. verrucosum in temperate climate and A. ochraceous in warm regions. In poultry feed materials, mycotoxins are found most commonly in cereals and to a lesser degree, in meals. The presence of OTA in animal feed contributes significantly to health disorders and decreased production. In addition to aflatoxins, which is an ubiquitously distributed toxin, OTA is one of the reasons for economic losses in the poultry industry due to poor performance and immunosuppression. Moreover, OTA has also been noted for the carryover …

Wright State University's Celebration Of Research, Scholarship And Creative Activities Book Of Abstracts From Friday, April 15, 2016, Wright State University Office Of Undergraduate Research And Stemm Activities

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Materials

The student abstract booklet is a compilation of abstracts from students' oral and poster presentations at Wright State University's Annual Celebration of Research, Scholarship and Creative Activities on April 15, 2016.

Poster: Embryonic Stem Cell Research, Emillie Partridge

Best Integrated Writing

This poster presentation by Partridge provides information about stem cell research and the controversy surrounding it.

Wsu Research News, Spring/Summer 2016, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A twenty-one page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.

Undergraduate And Graduate Course Descriptions, 2016 Spring, Wright State University

Course Descriptions

Wright State University undergraduate and graduate course descriptions from Spring 2016.

Flight Physician - March, 2016, Civil Aviation Medical Association

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A twenty-five page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Older Adults With Elevated Bmi Are At Greater Risk Of Accelerated Knee Osteoarthritis: Data From The Osteoarthritis Initiative, Kyle D. Wallace

Master of Public Health Program Student Publications

Background: Around 35% of adults with osteoarthritis (OA) have knee osteoarthritis (KOA), which generally progresses over several years; however, some individuals experience accelerated KOA (AKOA), a rapid progression to end-stage disease within 48-months. Purpose: To assess baseline differences among those who develop the different types of KOA, and to determine if baseline characteristics and measures can be used to predict an individual’s KOA status (Common KOA or AKOA) 48 months later.

Methods: Cross-sectional analysis of data from the Osteoarthritis Initiative (OAI) was completed. At baseline, 4,769 participants were enrolled. Data from individuals (n = 1,561) free of radiographic KOA (Kellgren-Lawrence …

A Set Of Smart Practices For Public Health Department Accreditation By Public Health Accreditation Board, Veer Raghava Kumar Marthy

Master of Public Health Program Student Publications

Purpose: The purpose of this research is to determine smart practices that could be used by a public health department to have an efficient, productive and successful accreditation by the Public Health Accreditation Board (PHAB).

Methodology: Accreditation coordinators of eight accredited Ohio-based public health departments were interviewed in 2016. A qualitative study was conducted using semi-structured interviews with funneling. Results were generated using a hybrid model of analysis that included a content analysis to make valid inferences from the interview data.

Results: The accreditation coordinators identified a series of smart practices. Six prominent themes that were identified and labeled as …

Final Report: Storm Water Pollutant Inputs To The Little Miami River Via Yellow Springs And Birch Creeks, Audrey E. Mcgowin

Chemistry Faculty Publications

This report describes the results of research performed with funds from the Sture Fredrik Anliot Grant Fund granted in March 2015. The primary aim of the project was to identify and measure various stormwater pollutant inputs to Yellow Springs Creek and Birch Creek into Glen Helen Nature Preserve and then into the Little Miami River in Greene County, Ohio from Spring 2015 to Winter 2016.

In order to better understand pollutant flow into Glen Helen and subsequently into the Little Miami River, three different types of samples were taken. One type of sample was a “grab” sample taken under normal …

Changes In Cytoskeleton Proteins In Hsv-1 Infection Of J774a.1 Macrophage Phenotype, Riham Abbas Subahi

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The cell cytoskeleton, a unique intracellular matrix found in all eukaryotes, is composed of three main protein structures, microtubules, microlaments and intermediate laments. The cytoskeleton maintains cell shape and internal structures providing mechanical support that facilitates intracellular transport (Parker et al., 2014). Many viruses such as the herpesviruses use the cytoskeleton system of the cell for infectivity (Henry Sum, M. S. 2015). HSV-1 utilizes the cell cytoskeleton in many steps of its life cycle from entry through assembly to egress (Lyman et al., 2008). During infection, HSV-1 viral proteins cause drastic changes and rearrangement of the cellular actin and cell …

Analyses Of Endothelial Cells And Endothelial Progenitor Cells Released Microvesicles By Using Microbead And Q-Dot Based Nanoparticle Tracking Analysis, Jinju Wang, Yun Zhong, Xiaotang Ma, Xiang Xiao, Chuanfang Cheng, Yusen Chen, Ifeanyi Iwuchukwu, Kenneth J. Gaines, Bin Zhao, Shiming Liu, Jeffrey B. Travers, Ji C. Bihl, Yanfang Chen

Pharmacology and Toxicology Faculty Publications

Accurate analysis of specific microvesicles (MVs) from biofluids is critical and challenging. Here we described novel methods to purify and detect MVs shed from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads with fluorescence quantum dots (Q-dots) coupled nanoparticle tracking analysis (NTA). In the in vitroscreening systems, we demonstrated that 1) anti-CD105 (EC marker) and anti-CD34 (EPC marker) conjugated-microbeads had the highest sensitivity and specificity for isolating respective MVs, which were confirmed with negative controls, CD41 and CD235a; 2) anti-CD144 (EC marker) and anti-KDR (EPC marker) conjugated-Q-dots exhibited the best sensitivity and specificity for their respective …

The Trend Of Communicable And Non-Communicable Diseases In East African Community (Eac) Countries: Case Study Of Burundi, Rwanda, And Uganda - 1990 To 2013, Emile Shumbusho

Master of Public Health Program Student Publications

Background: In low and middle-income countries (LMICs), non-communicable diseases (NCDs) are rapidly emerging alongside a well-documented persistence of communicable diseases (CDs) in fragile health care systems. In sub-Saharan Africa, chronic NCDs are expected to overtake CDs as the leading cause of death. The aim of this study was to compare mortality rates due to CDs and NCDs between developed countries, developing countries, Burundi, Rwanda, and Uganda from 1990 to 2013.

Methods: A descriptive study with longitudinal and cross-sectional analyses of mortality rates was conducted using the global burden of disease (GBD) study data. Tableau Public 9.3 software was utilized to …

Identification Of Protein-Protein Interactions Of Amyotrophic Lateral Sclerosis Associated Protein Tdp-43, Hanoor Sharma

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Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease caused by progressive degeneration of upper and lower motor neurons in the brain and spinal cord. Multiple mutations are found in some of the proteins associated with ALS, including superoxide dismutase (SOD1), fused in sarcoma (FUS) and trans-activation response DNA-binding protein (TDP-43). TDP-43 is a DNA and RNA binding protein, well conserved, and ubiquitously expressed in all tissues. TDP-43 resides in the nucleus and sometimes shuttles between nucleus and cytoplasm. Mutations in TDP-43 leads to mislocalization of TDP-43 to the cytosol where it was ubiqutinated and hyperphosphsorylated, ultimately leading to neuronal cell …

Adeno-Associated Virus-Vegf-165 Mediated Modification Of Adipose Derived Stem Cells For Cell Therapy, Upasana Niyogi

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Chronic wounds have become a major clinical and economic burden in our society. New approaches that accelerate wound healing are desperately needed. Angiogenesis and vascularization play a critical role in healing. One of the essential angiogenic factors that promote the formation of vascular beds is vascular endothelial growth factor (VEGF). Moreover, adipose-derived stem cells (ASC), through their regeneration and differentiation properties, may promote healing when transplanted into a wound bed. I have proposed to develop a novel organotypic wound model to facilitate the study of wound healing process. I hypothesize that administration of genetically-modified ASC secreting VEGF via an adeno-associated …

The Expression Of Aryl Hydrocarbon Receptor In Raw 264.7 Macrophages In The Presence Of Socs1 Peptide And Socs3 Peptide Mimetic And Cells Infected With Hsv-1, Maher Salem Alwethaynani

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Macrophages play a crucial role for our immune system and protect our body from infection. Suppressor of cytokine signaling (SOCS) proteins negatively regulate cytokine receptor and TLRs. The aryl hydrocarbon receptor (AhR) also performs an important role in immunity. This study investigated the changes in expression of AhR in RAW 264.7 macrophage cells after the addition of SOCS1 and SOCS3 peptide mimetics and also examined AhR expression in RAW 264.7 macrophage cells before and after the addition of HSV-1 RAW 264.7 murine macrophage cell lines which are from male BALB/c mice were used in this study. The addition of the …

The Evolution Of The Intersection Of Hiv And Breastfeeding In Global Policy, Kristin M. Sinning

Master of Public Health Program Student Publications

Increasing breastfeeding rates and reducing HIV/AIDS are two important global health priorities, and their intersection has led to policies that meet mutual goals of supporting, protecting and promoting breastfeeding and reducing the risk of HIV/AIDS. The World Health Organization has developed and released recommendations, policies, and guidance on infant feeding and the risk of transmission through breastfeeding. This analysis provides a historical, chronological perspective on the global policies for infant feeding in the case of an HIV-positive mother, an analysis of changes, and recommendations for the future. The policies evaluated demonstrate the evolution in knowledge and recommendations for infant feeding …