Digital Commons Network^™

Neuromuscular Performance In Strength Or Endurance Trained Individuals: Construct Validity Of Mcpet Variables, Jeff Moore, Paolina Chavez Ibarra, Daniel T. Cannon, Harry B. Rossiter Facsm, Carrie Ferguson

International Journal of Exercise Science: Conference Proceedings

The musculo-cardiopulmonary exercise test (mCPET) assesses neuromuscular performance during a standard ramp-incremental cycle ergometer exercise test using the addition of brief (5s), maximal effort isokinetic cycling power (Piso) measurements at baseline and intolerance. mCPET quantifies 4 additional variables: maximum voluntary isokinetic power (Baseline Piso); the fraction of Baseline Piso achieved at V̇O₂peak (Aerobic Power Index, API); neuromuscular fatigability between baseline and intolerance Piso (Fatigue Index, FI); and the difference between peak ramp power and Piso at intolerance (Power Reserve, PR), reflecting the degree neuromuscular performance limits the exercise. PURPOSE: To determine construct validity of the mCPET using …

User Experience On Virtual Reality On Motor Learning And Real World Skill Transfer Of A Novel Forearm And Hand Prosthesis, Kenneth M. Mazariegos, Jacob Hinkel-Lipsker

International Journal of Exercise Science: Conference Proceedings

Virtual Reality is a medium that growing as a means for rehabilitation practices, allowing patients to receive personalized training scenarios with minimal equipment and space requirements. However, there is still a need to understand how different aspects of VR design such as physical fidelity (visual realism), cognitive fidelity (psychological realism), and adaptivity (whether the challenge of the VR task scales to user proficiency) influence learning and enhancement of motor skills. PURPOSE. To evaluate the impact of physical and cognitive fidelity in a VR environment on motor learning and real-world skill transfer of a reaching task using a novel arm prosthesis. …

Time-Of-Day And Sex-Dependent Effects Of Exercise On Cardiometabolic Health In Mice With Social Jetlag, Graham R. Mcginnis

International Journal of Exercise Science: Conference Proceedings

Physiology has adapted to function on a 24 hour time scale, referred to as the circadian rhythm. Operating outside of this time scale leads to circadian rhythm disruption, which invariably leads to pathological outcomes. Social jetlag (SJL), a common form of circadian rhythm disruption, occurs when an individual’s sleep/wake schedule differs throughout the week (i.e. – staying up late on the weekends), and has been associated with obesity and cardiovascular disease. Exercise is a potential intervention to prevent SJL-induced impairments, but the time of day at which exercise is most effective is unknown.

PURPOSE: Determine if early or late phase …

Heart Rate Differences Between Elite And Casual Super Smash Bros. Ultimate Players Across Tournament Phases, Bailee Costa, Briant Jacobo-Ceja, Dillon Harris, Zachary R. Mcfadden, Steven B. Machek

International Journal of Exercise Science: Conference Proceedings

Super Smash Bros Ultimate (SSBU) is a prominent title in the expanding Esports industry, whereby players are potentially placed under unique neurophysiological demands that have been partially attributed to enhanced blood flow. Moreoever, the influence of player skill and tournament-specific situations on these outcomes are sparsely described. PURPOSE: to examine the within- and between-group differences in heart rate (HR) between elite-level (5 ES; internationally-ranked) and casual (6 CAS; plays > 4 hr/wk) SSBU players throughout their participation in double-elimination tournaments. METHODS: After screening and baseline assessments, participant HRs were fitted with an H10 Polar chest-based monitor and individual HR …

Evaluating Takeoff Mechanics Between Dancers And Non-Dancers During Bilateral Jumps, Danielle Serrano, Nixom Alfaro, Ricardo Pineda, Stephana Powell, John Reed, Ricardo Tolentino, Danielle Jarvis

International Journal of Exercise Science: Conference Proceedings

Many athletes in sports that consist of movements with sharp turns, such as women's basketball and soccer, experience a high amount of anterior cruciate ligament (ACL) injuries in proportion to dancers, who experience low rates of ACL injuries. There has been minimal research on how jump takeoff mechanics differ between dancers and other active individuals. Some previous work has indicated that dancers use greater motion and forces at the ankle joint compared to non-dancers in jumping movements. PURPOSE: To determine the kinetic and kinematic differences between non-dancers and dancers during bilateral squat jump takeoffs. METHODS: Nineteen healthy female …

Acute Sleep Reduction Impairs Heart Rate Variability And Heart Rate Recovery Following Aerobic Exercise In Healthy Adults, Jocelyn Kaveney, Gracie Dupuis, Eric Lessard, Braden Wilson, Grahm Mcginnis, Ryan Perkins

International Journal of Exercise Science: Conference Proceedings

INTRODUCTION: According to the CDC, ~35% of American adults are receiving an inadequate amount of sleep (/night). PURPOSE: This study aimed to investigate the effects of acute sleep reduction on heart rate variability (HRV) and heart rate recovery (HRR) following aerobic exercise. METHODS: Healthy, active adults (n=11; 26.4±5.4 yr; 45% female) were screened to ensure eligibility (i.e., meeting the CDC’s sleep recommendations of 7-9hr/night, apparently healthy, recreationally active, and non-smoking), characterize body composition via DXA and measure maximal cardiovascular capacity (VO2max). Utilizing a within subjects, repeated measures design, participants completed an initial 7d period of habitual sleep (CON) followed …

Landing Mechanic Differences Between Dancers And Non-Dancers During Vertical Jumps, John Reed, Jenelle Idian, Jasmin De La Cruz, Andrea Rodriguez, Aysha W. Cunningham, Ricardo Tolentino, Danielle Jarvis

International Journal of Exercise Science: Conference Proceedings

Dancers endure training that results in an increased range of motion and flexibility. Such training can impact risk of injury and produce overall differences in movement. Despite these potential impacts, there is minimal research comparing landing mechanics between dancers and non-dancers. PURPOSE: To explore kinetic and kinematic differences between dancers and non-dancers when landing from a vertical jump. It was hypothesized that dancers would demonstrate greater peak ankle dorsiflexion and ankle net joint moments during landing compared to non-dancers. METHODS: A sample of ten dancers and nine non-dancers, all healthy females, were equipped with reflective markers and performed …

Effect Of Load On Lower Extremity Joint Torques In Rear Foot-Elevated Split Squats, Daniel R. Trounday, Miranda Chan, Connor Dominici, Michele Leblanc Facsm

International Journal of Exercise Science: Conference Proceedings

Joint torques provide information regarding the net contribution of each joint during a movement, which is useful to know in training and rehabilitation settings. PURPOSE: To investigate the effect of load on joint torques during rear foot-elevated split squats (RFESS) relative to body mass and to total mass (body mass + weights). METHODS: Fourteen injury-free males, ages 18-25, who regularly performed lower body resistance training participated. A 1RM test was conducted in a previous session to determine each subject’s RFESS 1RM. Each participant stepped on a force plate with their right leg, with their left leg supported …

Comparison Of Data Cleaning Procedures For Gastrointestinal Temperature In An Occupational Setting, Hillary A. Yoder, Anne M. Mulholland, Jonathan E. Wingo, Lyndsay K. Krisher, Yaqiang Li

International Journal of Exercise Science: Conference Proceedings

Gastrointestinal temperature (Tgi) is measured in occupational settings to monitor heat stress, but Tgi telemetric pill sensors are often ingested without sufficient time to exit the stomach, increasing the likelihood of errors and thereby necessitating data cleaning. It is unknown if data cleaning method affects the interpretation of heat stress. PURPOSE: The purpose of this study was to compare 2 recently published methods of cleaning Tgi data. METHODS: Tgi was measured once every 5 min during work from 6:00 am – 2:30 pm in 19 people (13 on Day 1 and 17 on Day 2). Data were cleaned using the …

The Effect Of Prolonged Bed Rest On Muscle Oxidative Capacity And Muscle O₂ Diffusive Sensitivity In Young And Elderly Participants, Alessio Marciano, Andrea Pilotto, Alessandra Adami, Harry B. Rossiter Facsm, Simone Porcelli

International Journal of Exercise Science: Conference Proceedings

Whole body maximum oxygen uptake (V̇O_2MAX) is dependent on the capacity for convective and diffusive oxygen transport and skeletal muscle O₂ extraction. V̇O_2MAX declines after bed rest (BR), accompanied by reductions in convective O₂ transport and muscle oxidative capacity. The effect of BR on diffusive O₂ transport limitation is unknown.

PURPOSE: To determine vastus lateralis (VL) oxidative capacity and limitation in O₂ diffusion at skeletal muscle level in young (Y) and elderly (E) participants after 21 and 10 days of BR, respectively.

METHODS: 9 Y (23±4 yrs) and 10 E (69±3 …

Effect Of Caffeine On Acute Kidney Injury Markers During Simulated Occupational Work In The Heat, Jennifer M. Raef, Jonathan W. Specht, Marissa R. Ramirez, Amber Quintana, Alyssa R. Bailly, Ariel Pena, Ethan Schmidt, Flavio De Castro Magalhaes, Zachary J. Mckenna, Zachary J. Schlader Facsm, Fabiano T. Amorim

International Journal of Exercise Science: Conference Proceedings

Caffeine is a stimulant widely used by workers in labor-intensive occupations to enhance mood and concentration. Previous research shows that caffeine has a diuretic effect and may increase heat production during activity in the heat, potentially contributing to dehydration and hyperthermia. The prevalence of dehydration and hyperthermia is high in workers who are exposed to hot conditions for extended durations, putting them at risk for acute kidney injury (AKI). Despite the high prevalence of caffeine use, the possible effect on AKI is unknown. PURPOSE: To investigate the effect of caffeine on markers of kidney function and AKI risk during …

Validation Of Polar Sense Monitor Over Tattooed Skin, Rodolfo I. Mejia

International Journal of Exercise Science: Conference Proceedings

The prevalence of tattoos has increased over the years, and, correspondingly, wearable technology, especially heart rate wearables. The signal from the heart rate wearables may be affected by the permanent ink on the skin because of previous validation studies excluding people with tattoos. PURPOSE: Examine the validity of heart rate monitors on people with arm tattoos. METHODS: 20 participants were recruited for this study. There were three conditions in the study that included heart rate measurements at rest, self-paced walking, and self-paced jogging. The Polar H10 and the Polar Verity Sense armband were used as the heart rate monitors. The …

Effects Of Booties On Foot Skin Temperature During Surfing, Juan Leyva, Richard Armenta, Jeff A. Nessler, Sean Newcomer

International Journal of Exercise Science: Conference Proceedings

Cold water exposure during surfing can induce cold stress which can potentially impact comfort and performance. Neoprene booties have been marketed to mitigate cold stress within the feet, but there is limited information regarding their effectiveness PURPOSE: The purpose of this study was to characterize dorsal and plantar foot skin temperatures between two bootie models (strapped vs. strapless) during sixty minutes of recreational surfing in cold water. METHODS: Thirty-six recreational surfers (25 males and 11 females) with foot sizes between 7 and 10 each wore one strapped and one strapless 3 mm neoprene bootie that were randomized between left and …

Perceived Menstrual Cycle-Related Symptoms Throughout Four Cycles In Elite South American Futsal Athletes., Abigail Larson, Catalina Gamboa

International Journal of Exercise Science: Conference Proceedings

ABSTRACT

Previous research suggests menstrual cycle (MC) phases have minimal impact on exercise performance; however, less is known about the influences of MC phases on day-to-day training variables, particularly those that are subjective in nature. Mobile apps designed to track MCs are becoming increasingly popular for exercise planning but the utility of these tools for capturing MC-related changes in training/performance variables has not been fully explored. PURPOSE: This study aimed to identify how the four phases of the MC (menstrual, follicular, ovulatory, and luteal), predicted by the Fitr Woman App, influence the frequency of daily, self-reported perceptions of decreased …

Exploring The Physiological Limits Of Aging: A Case Study Of The Male 50-Km 80+ World Record Holder, Andrea M. Pilotto, Enrique Higueras-Liébana, Massimiliano Ansaldo, Marianna Neri, Letizia Giusti, Ivan Baltasar-Fernandez, Ángel Buendía-Romero, Pedro Luis Valenzuela, Ignacio Ara, Julian Alcazar, Simone Porcelli

International Journal of Exercise Science: Conference Proceedings

In literature, age-related changes in cardiorespiratory fitness and endurance performance are questioned due to potential confounding effects from modifications in exercise training. World-class master athletes serve as exceptional models for understanding high-level physical training impact in older age.

PURPOSE: To examine physiological and performance profiles of a male 81-year-old master runner who in 2025 set a new 80+ world record in the 50-km race (4h47m39s, 10.5 km·h^-1).

METHODS: Two weeks after the new world record, maximal O₂ uptake (V̇O_2max), the fractional utilization of V̇O_2max (lactate threshold - LT) and running economy (RE) …

Phase Angle As An Indicator Of Cellular Health And Body Composition In Active Older Adults, Paxton Castillon, Heather Feeny, Abby Hansen, Alyssa Mcnutt, Matthew Densley, Elizabeth Cafferty, Ben Taylor, Nicolas Clark, Andrew Creer, Dale Wagner Facsm

International Journal of Exercise Science: Conference Proceedings

BACKGROUND: Phase angle (PhA), derived from bioelectrical impedance analysis, reflects cellular health and membrane integrity. It is influenced by muscle mass, hydration, and cell membrane function, making it particularly relevant for assessing physiological status in older adults.

PURPOSE: The purpose of this study was to examine relationships between PhA and body composition variables in active older adults participating in the Huntsman Senior Games.

METHODS: This observational descriptive cross-sectional study included 504 participants (307 women, 197 men; mean age 66.3 ± 7.9 years). Data were collected using the InBody 770 multifrequency body composition analyzer at the Huntsman Senior Games in St. …

Examining Muscle Synergy Deficits In Rotator Cuff Tendinopathy, Nicholas K. Odani, Chethan N. Reddy, Daniel C. Mcpherson, Kayla D. Seymore, Lori A. Michener

International Journal of Exercise Science: Conference Proceedings

Neuromuscular deficits observed through electromyography (EMG) of individual muscles are common in rotator cuff (RC) tendinopathy. However, given that muscles act in coordination, muscle synergy analyses can identify altered neuromuscular patterns that can inform specific interventions to address these deficits.

PURPOSE: Characterize shoulder muscle synergies in RC tendinopathy.

METHODS: 54 individuals with RC tendinopathy (32M, 29.4 ± 8.9 yr) and 54 healthy controls (matched for age, sex, height, weight, and hand dominance) completed four velocity-controlled shoulder elevation tasks with seven repetitions of scaption, flexion, forward reaching, and contralateral reaching. During each task, muscle activity was collected from the …

Who Chooses To Move? A Snapshot Of University Students Enrolled In Physical Activity Courses, Zakkoyya H. Lewis, Ami Rollins, Madeline Gutierrez, Emily Condon, Nicolas Ovalle

International Journal of Exercise Science: Conference Proceedings

Physical activity rates of emerging adults in the United States declines significantly during the transition to university. The university environment can introduce new barriers that prevent students from prioritizing their physical fitness; however, there are other students that make physical activity a priority and embed it into their academic curriculum. PURPOSE: The purpose of this study is to provide the characteristics of a sample of university students enrolled in a physical activity course. METHODS: Students registered for a Weight Training and Kickboxing activity course voluntarily enrolled in a study evaluating the impact of enjoyment on physical activity. Participants completed a …

Implementing Exercise Is Medicine At Byu, Destiny Rose Romero, Allen C. Parcell, Ron Hager, Mandy Christensen

International Journal of Exercise Science: Conference Proceedings

Recognizing the disconnect between the knowledge of exercise’s utility in medicinal treatment and the lack of exercise prescription in practice, multiple researchers have conducted studies to evaluate practitioner attitudes and experience towards discussing physical activity. Implementing Exercise is Medicine at BYU Student Health Center (IRB2024 – 294) is one such study directed towards Brigham Young University’s integrated health center. PURPOSE: The purpose of the study was to investigate the likelihood of implementing Exercise is Medicine at the Student Health Center (SHC) with the intent of identifying barriers to implementation and brainstorming actionable solutions. METHODS: The electronic survey titled …

Upper Extremity Biomechanical Analysis Of Defensive Flag Pulling In Flag Football, Rianne L. Aguilar, Claudia De La Cruz, Travis J. Peterson

International Journal of Exercise Science: Conference Proceedings

PURPOSE: This project aimed to determine upper extremity techniques during defensive flag pulls in women’s flag football. METHODS: Eleven healthy, female flag football players with at least high school varsity experience volunteered to participate in this study. Participants were instructed to pull the flag(s) of a stationary dummy placed 3 yards away as fast as possible. Trials were conducted in flags facing forward (F), angled 45° left (L), and angled 45° right (R) of the flag puller. Motion capture (Vicon, 120 Hz) and video analysis were used to analyze technique. The Waterloo Handedness Survey was used to indicate hand preference. …

Influence Of Virtual Reality Cognitive Fidelity On Biomechanical Adaptation And Motor Strategies With A Custom Prosthesis And Foot Controller., Marek R. Kwasek

International Journal of Exercise Science: Conference Proceedings

In an age of digitization, rehabilitation is embracing newer technologies, particularly VR, to enhance motor development and learning skills with real-world applications. With its practical replicability, VR’s visual (i.e., how visually realistic a virtual environment is) and cognitive fidelity (i.e., how well a virtual environment mimics the cognitive demands of real-world tasks) has the potential to influence motor strategies and, ultimately, a person’s functional biomechanics when learning a new skill. PURPOSE: To examine how different levels of visual and cognitive fidelity impact users’ reaching biomechanics when controlling a prosthetic arm using a foot controller. METHODS: Participants completed 12 …

Nanomechanical Effects Of Microgravity On Murine Tendon Fibrils, Patrick Bialy, Andrew S. Ichimura, Kent A. Lorenz, Ryan T. Scott, James R. Bagley

International Journal of Exercise Science: Conference Proceedings

Microgravity, characterized by a weak gravitational pull, poses physiological risks to astronaut health and performance. While its impact on muscle and bone is well documented, effects on connective tissue, such as tendon, remain understudied. PURPOSE: This study aimed to 1) quantify the effects of 37 days of microgravity on the compressive nanomechanics of individual collagen fibrils and 2) compare these findings to existing literature and previously collected lab data. We hypothesized that spaceflight (SF) fibrils would exhibit a lower Young’s Modulus (YM) than ground controls (GC). METHODS: Female C57BL/6J mice were exposed to 37 days of spaceflight aboard the International …

The Effect Of Birkenstock® Arizona Sandals On Reducing Gait Changes Linked To Plantar Fasciitis For Level And Incline Walking, Julian D. Macdonald, Christiane R. O'Hara, Scott Hazelwood

International Journal of Exercise Science: Conference Proceedings

Birkenstock^® Arizona sandals have seen a surge in popularity among college aged students, though limited research is available regarding their effect on foot biomechanics. Understanding the changes to biomechanical parameters caused by footwear choice is valuable information for these footwear users. PURPOSE: The purpose of this study was to analyze specific biomechanical parameters linked to the development of plantar fasciitis that result from wearing Birkenstock^® Arizona sandals. These parameters included ankle dorsiflexion, eversion, compressive force, anterior displacement of the foot center of pressure, and lower leg muscle activation. METHODS: 6 healthy college aged male participants were recruited to …

Emg Analysis Of The Upper Body During Different Cycling Positions, Nicholas G. Cariquitan, Amara Dumas, Christiane R. O'Hara

International Journal of Exercise Science: Conference Proceedings

Elite, novice, and beginner cyclists all encounter varying positions on the bicycle to meet comfort and/or performance needs. These positions must take into account the complex relationship between muscular activation, flexibility, and the limits of human biomechanics. PURPOSE: To investigate three bicycle positions and the effect of the rider’s upper body muscular activation and comfort. The bicycle position was defined by hip angles: normal (120-130 degrees), aggressive (110-115 degrees), and upright posture (140-150 degrees), while maintaining a constant knee angle (28-32 degrees). METHODS: 10 college-aged participants with cycling experience from Cal Poly San Luis Obispo and the surrounding area were …

Effects Of Acute Sleep Reduction And Aerobic Exercise On Metabolic Function In Healthy Adults, Eric Lessard, Gracie Dupuis, Jocelyn Kaveney, Braden Wilson, Grahm Mcginnis, Ryan Perkins

International Journal of Exercise Science: Conference Proceedings

INTRODUCTION: The number of adults who receive insufficient sleep is progressively increasing, impacting the established relationship between sleep and metabolic function. PURPOSE: The purpose of this study was to explore the effects of acute sleep reduction on metabolic variables at rest and during aerobic exercise. METHODS: Healthy, active adults (n=11; 26.4±5.4 yr; 45% female) were screened to ensure eligibility (i.e., meeting the CDC’s sleep recommendations of 7-9hr/night, apparently healthy, recreationally active, and non-smoking), characterize body composition via DXA and measure maximal cardiovascular capacity (VO2max). Utilizing a within subjects, repeated measures design, participants completed an initial 7d period of …

Correlational Analysis Between Fat-Free Mass And Physical Performance In College Students, Rhea Misra, Giselle Murillo, Fraz Tahir, Tianou Zhang

International Journal of Exercise Science: Conference Proceedings

Fat-free mass (FFM), the body’s metabolically active tissue excluding fat, plays a critical role in strength, endurance, and overall physical performance. PURPOSE: The objective of this study is to investigate the association between FFM and physical performance in college students. METHODS: FFM was evaluated using the BodPod in a sample of 21 male and 12 female students. Various performance metrics were measured, including handgrip strength, aerobic capacity (via VO₂max test), anaerobic capacity (peak power and peak force through Wingate test), vertical jump height, and broad jump distance. Spearman correlations were used to evaluate correlations between FFM and performance …

Interleukin-6 As A Mediator Of Circadian And Sex Differences In Exercise-Induced Stat3 And Autophagy Signaling, Srushti Wagh, Ariana Khzarjyan, Joshua Corpuz, Elias M. Malek, Charli Aguilar, Michael Dial, Paula Martinez, Graham Mcginnis

International Journal of Exercise Science: Conference Proceedings

Circadian rhythms are highly conserved across tissues and play an important role in regulating how the heart responds to physiological and pathological stress. Exercise is known to be cardioprotective, yet the impact of exercise timing on molecular signaling is still being explored. Interleukin-6 (IL-6) is an exercise-induced cytokine which has been shown to be important to cardioprotection and metabolism. It is currently unknown how exercise timing modulates the IL-6 signaling pathway in the heart.

PURPOSE: To investigate how time-of-day and IL-6 influence exercise-induced activation of STAT3 and its downstream signaling pathways in male and female mouse hearts.

METHODS: Wild-type and …

Comparison Of Lower Limb Sagittal Plane Kinematics Measured Using Opencap And Traditional Mocap During Single Limb Squat Analysis In Patients Post-Aclr, Alexander Chen, Jiaqi Wang, Susan Sigward, Willa Ma, Grace Choi

International Journal of Exercise Science: Conference Proceedings

OpenCap (Stanford University) uses standard smartphone cameras and computer vision algorithms to estimate joint kinematics without markers. With affordable equipment and reasonable setup procedures, it may provide an alternative to visual assessment of movement impairments in the clinic. Subtle alterations in joint kinematics can be relevant to the interpretation of recovery progress following musculoskeletal injury or surgery such as anterior cruciate ligament reconstruction (ACLr). It is important to understand the level of agreement between new tools for assessment and gold standard kinematic measures. PURPOSE: To determine concurrent validity of kinematic data collected using OpenCap and gold standard technologies during …

The Effect Of Fatigue On Hip, Knee, And Ankle Kinetics During Rear Foot-Elevated Split Squats, Sara C. Mills, Jaime Alvarado, Tyler Lindholm, Michele Leblanc Facsm

International Journal of Exercise Science: Conference Proceedings

Rear foot-elevated split squats (RFESS) are a widely used movement to increase lower body strength, but little is known about how fatigue alters joint torque demands across the ankle, knee, and hip. Previous research has shown that fatigue changes kinematics during RFESS, increasing hip and knee motion while reducing ankle movement, but the effect on joint torques remains unclear. PURPOSE: To examine the effects of fatigue on ankle, knee, and hip torque in the sagittal and frontal planes during the eccentric and concentric phases of the RFESS in active females aged 18–25. METHODS: Participants performed repeated body weight RFESS until …

High-Dose Sodium Supplementation On Hydration And Thermoregulatory Responses While Cycling In The Heat, Melani Kelly

International Journal of Exercise Science: Conference Proceedings

Sodium is commonly included in sports beverages to promote hydration and support carbohydrate (CHO) absorption, typically at concentrations of ~270-400mg. Recently, high-dose sodium supplements, often approaching 1000mg and lacking CHO, have entered the market with minimal evidence to support their use. PURPOSE: Investigate how hydration and thermoregulatory responses change during cycling in the heat when consuming a supplement containing 810mg of sodium. METHODS: Twenty recreationally active participants (15 males, 5 females; age = 23.4 ± 2.8) completed a 2-h bout of cycling in an environmental chamber (37.3℃, 59.1 ± 4.4% relative humidity) on two occasions separated by > 7 …