Digital Commons Network^™

Development Of A Dna Biosensor Using Crispr/Dcas9 On A Graphene Oxide Surface For Detecting Antibiotic Resistance Genes, Wendy Cecil

Mahurin Honors College Capstone Experience/Thesis Projects

Antibiotic resistance is a rapidly spreading global threat to human health. Previously in our lab, we developed a simple method for facile detection of antibiotic resistance genes (ARGs) in bacteria. This DNA detection system takes advantage of the CRISPR-Cas system (Clustered Regularly Interspaced Short Palindromic Repeat and CRISPRAssociated Protein). We designed CRISPR in complex with nuclease deactivated Cas9 (dCas9) on graphene oxide (GO) sheets conjugated with Cas9 antibodies. Single guide RNA (sgRNA) was customized to program dCas9 for target DNA binding. sgRNA was covalently labeled with fluorescein to provide fluorescence signal. Graphene oxide (GO) is utilized for its quenching properties …

Beyond Sustainable: An Analysis Of The Visual Rhetoric Behind The Branding Of Christy Dawn, Megan Mccormick

Mahurin Honors College Capstone Experience/Thesis Projects

This research project offers insight into the visual rhetorical of design decisions made in the marketing materials of Christy Dawn, a sustainable fashion brand. The primary goal of this research is to identify several motivators, based on prior studies, for the consumption of sustainable fashion and analyze the existing marketing visuals of Christy Dawn’s brand, specifically in how they appeal to these motivators, thus also communicating their sustainable rhetoric to consumers in the process. This project defines these motivators in detail and addresses them in relation to particular elements of design in Christy Dawn’s materials, such as images, color, and …

Antibiotic Resistant Gene Concentrations In Bacteria In Groundwater From Agricultural Waste, Koji Barnaby

Mahurin Honors College Capstone Experience/Thesis Projects

The overuse of antibiotics has led to an increase in antibiotic resistant bacteria since some antibiotics are sent into the wastewater system by waste produced by both humans and agriculture, making exposure to these bacteria more likely. The evolution of these bacteria in groundwater is of particular concern, as groundwater is used as a source of drinking water, and infections by these bacteria would be more difficult to treat. There are no policies in place to monitor or regulate antibiotic resistance bacteria in groundwater, leaving the threat to public health unknown. The study area of Crumps Cave in Smiths Grove, …

Ua68/14 Newsletter, Wku Folk Studies & Anthropology

WKU Administration Documents

Newsletter created by and about WKU Folk Studies & Anthropology.

• Applegate, Darlene. From the Department Head & Anthropology Program Director
• Ferrell, Ann. From the Folk Studies Program Director
• Student Profiles - Georgia Dassler, Camille Acosta
• Student Profiles - Azadeh Najafian, Corbin Pike
• Student Profiles - James Skaggs, Jordan Mansfield
• Student Projects
• This Year's Graduates
• Department Events & Activities
• Faculty/Staff News - Tim Frandy, Darlene Applegate, Ann Ferrell
• Faculty/Staff News - Gwynn Henderson, Erika Brady, Jean-Luc Houle, Kate Hudepohl, David Pollack, Justin Carlson
• Kentucky Folklife Program
• Kentucky Archaeological Survey
• Alumni Updates

Ua1f Kern Alexander Censorship Vertical File, Wku Archives

WKU Administration Documents

Digitized vertical file materials regarding WKU President Kern Alexander's interactions with the College Heights Herald.

Ua19/16/1/5 Hilltopper Golf 2020-21 Season Statistics, Wku Athletic Media Relations

WKU Administration Documents

Statistical analysis of the 2020-21 men's golf team season.

Ua3/1/1 President's Office-Cherry Reports, Wku Archives

WKU Archives Collection Inventories

Reports made by Henry Cherry to the Board of Regents and departmental reports made to Henry Cherry.

Ua25/4 Finance & Administration - Vice President, Wku Archives

WKU Archives Collection Inventories

Records created by and about the Vice President of Finance & Administration.

Ua19/16/1 Lady Topper Basketball Media Guide, Wku Athletic Media Relations

WKU Administration Documents

2021-22 women's basketball media guide produced by WKU Athletic Media Relations, includes athletic records and statistics, photographs, schedule and information regarding opponents.

Ua1f Kern Alexander Vertical File Part 3, Wku Archives

WKU Administration Documents

Digitized vertical file materials regarding WKU President Kern Alexander and his tenure as president 1986-1988.

Ua68/17 Wku Dance: Creating, Thinking, Artists, Wku Theatre & Dance

WKU Administration Documents

Newsletter created by and about the WKU Dance program.

Ua19/16/2 Softball Press Releases, Wku Athletic Media Relations

WKU Administration Documents

Press releases, photos and game statistics for WKU softball team in 2021.

Ua19/16/2 Volleyball Press Releases, Wku Athletic Media Relations

WKU Administration Documents

Press releases, photos and game statistics for WKU volleyball team in 2021.

Ua19/16/1 Volleyball Media Guide, Wku Athletic Media Relations

WKU Administration Documents

Athletic media guide for volleyball team.

Ua12/8 Annual Campus Safety & Security Report, Wku Police

WKU Administration Documents

A statement of current campus policies regarding procedures for students and others to report criminal actions or other emergencies occurring on campus and policies concerning the institution's response to such reports.

Effective Community Engagement Programs In Contemporary Concert Dance Companies, Emily Caldwell

Mahurin Honors College Capstone Experience/Thesis Projects

Arts education is extremely important yet underrated and underfunded in our country today. Some professional dance companies provide educational opportunities in dance and other areas of the arts for youth and others within the community as a means to combat this problem. This project is a compilation and synthesis of my research on how to most effectively create and implement children’s outreach programs in contemporary modern concert dance companies. The purpose of this research is to study how professional dance companies give back to their communities through engagement with children via classes, performances, workshops, etc. and to determine the most …

A Review And Comparison Of U.S. State Wildlife Action Plans For Stonefly (Insecta, Plecoptera) Species Of Greatest Conservation Need, Kathryn Greene

Mahurin Honors College Capstone Experience/Thesis Projects

State Wildlife Action Plans (SWAPs) were created in order to identify Species of Greatest Conservation Need (SGCN) and develop plans to prevent their populations from further decline and the possibility of being listed as threatened or endangered. Which SGCNs are included are decided based on characteristics that determine their vulnerability. As of early 2021, approximately 720stoneflies (Insecta, Plecoptera) species are found in the U.S., but only 143 are listed as SGCN. Only 29 states have stoneflies included on their SGCN lists, but 37 states use EPT (Ephemeroptera + Plecoptera + Trichoptera) metrics when assessing water quality issues in running water …

Autism And The Foster Care System In Kentucky, Caroline Clark

Mahurin Honors College Capstone Experience/Thesis Projects

According to the CDC, one in 59 children are diagnosed with Autism Spectrum Disorder (Centers for Diseases and Prevention, 2020). More than 10% of those in the foster care system also have an autism diagnosis. The purpose of this project was to understand the general public’s knowledge and beliefs about both subjects. I conducted this study to identify any misconceptions, assumed facts, and opinions about both foster care and autism. I collected these data through anonymous surveys. The data were then analyzed and examined to create an app to benefit current and future foster parents who foster individuals with autism.

Using Machine Learning To Interpret Dice Rolls, Hunter Wimsatt, Aarohi Panzade, Kaaustaaub Shankar, Warren Campbell

SEAS Faculty Publications

Gamers use polyhedral dice that come with 4 sides (D4), 6 sides (D6), 8 sides (D8), 10 sides (D10), 12 sides (D12) and 20 sides (D20). All dice are unfair, some more than others. The goal of this project was to develop a machine learning and computer vision solution for the interpretation of dice rolls. When combined with an automated dice roller it would facilitate the study of dice unfairness. In the machine learning literature, Convolutional Neural Networks (CNNs) are the preferred method of computer classification of images. The CNN convolves images with different weights and biases through multiple layers …

Ua12/2/67 Alpha Kappa Alpha, Wku Archives

WKU Archives Collection Inventories

Records created by and about Alpha Kappa Alpha sorority.

Ua12/2/68 Sigma Nu, Wku Archives

WKU Archives Collection Inventories

Records created by and about Sigma Nu fraternity.

Ua42/3 Delo Extended Campuses, Wku Archives

WKU Archives Collection Inventories

Records created by and about the extended campuses.

• Subseries 1 WKU Elizabethtown / Ft. Knox
• Subseries 2 WKU Glasgow
• Subseries 3 WKU Owensboro

Ua42/3/2 Delo Wku Glasgow, Wku Archives

WKU Archives Collection Inventories

Records created by and about the extended campus of WKU Glasgow.

Ua1c11/103 - Wku President's Office Photo Collection, Wku Archives

WKU Archives Collection Inventories

Photographs removed from WKU President's Office papers.

Ua42/1 Delo Academic Outreach, Wku Archives

WKU Archives Collection Inventories

Records created by and about Academic Outreach.

• Subseries 1 Summer Sessions & Winter Term
• Subseries 2 Independent Learning
• Subseries 3 Scholastic Development
• Subseries 4 Community College
• Subseries 5 WKU Online / Online Program Services
• Subseries 6 Distance Learning
• Subseries 7 Cohort Programs
• Subseries 8 Study Away

Dual Credit & Online Program Development / Compliance are part of this unit.

Alfred Russel Wallace Notes 15. Wallace's Many "Hats": What Should We Call Him?, Charles H. Smith

Faculty/Staff Personal Papers

Alfred Russel Wallace (1823−1913) has been referred to by name through the aid of a variety of labels – some thirty or more, in fact – that link him to his emphases of attention. How many of these labels are/were justifiable? The assessment here is that he is probably best remembered as a ‘naturalist,’ ‘geographer,’ ‘evolutionist,’ and ‘social critic.’

Centralizing The Purse: Why A More Centralized National Government Was Crucial For Managing America's Revolutionary War Debt, Ethan Berg

Mahurin Honors College Capstone Experience/Thesis Projects

Research on the American Revolutionary debt has particularly focused on the leadership of American financial figures: most notably Superintendent of Finance Robert Morris and U.S. Treasury Secretary Alexander Hamilton. While scholars affirm their indispensable leadership, they also acknowledge institutional and political conditions that distinguish their tenures. This CE/T seeks to further analyze these conditions. It draws upon scholarly works while evaluating different aspects and time frames of Americas’ public debt, including the initial accumulation of debt, the tenure of Robert Morris, the formation of the U.S. Constitution, and the tenure of Alexander Hamilton. The CE/T also analyzes the papers of …

Hallucinogenic And Sedative Drug Treatment And Its Impact On Mental Health Nursing, Meredith Cherry

Mahurin Honors College Capstone Experience/Thesis Projects

Mental health nursing is a complex and radically changing discipline that must continuously adapt to evidence based practices, including the use of alternative options for the treatment of mental illness. There has recently been increased research into the use of hallucinogenic and sedative drugs for the treatment of mental illness. Hallucinogenic and sedative drugs were examined as potential treatment options for a variety of mental illnesses in the 1960s, although these trials were ceased by the government due to human safety concerns. The United States Food and Drug Administration (FDA) has recently approved trials to study the use of hallucinogenic …

Wind Tunnel Data Acquisition System Development Using Labview, Riley Bishop

Mahurin Honors College Capstone Experience/Thesis Projects

Utilizing an educational-purpose wind tunnel, a data acquisition system through LabVIEW was created to experimentally analyze the aerodynamic forces experienced by objects in uniform external flow by automatically performing trials and interpreting electrical measurement signals. To verify experimental results from pressure distribution data around an object, a force balance was designed to mount objects stationarily in the wind tunnel test section while directly measuring the total lift and drag forces, calculating the pitching moment (for airfoils), and monitoring the angle of attack. The force balance design includes three load cells, one for measuring drag force and two for measuring total …

Public Perceptions Of Language Education In Taiwan: English In A Multilingual Context, Isabel Eliassen

Mahurin Honors College Capstone Experience/Thesis Projects

Language policy in Taiwan has been a focus of the government since the days of the Kuomintang (KMT) dictatorship, when languages other than Mandarin were ruthlessly suppressed. After democratization, local languages were allowed again, but soon English became Taiwan’s top language education priority. However, not all Taiwanese agree with the decision to prioritize English. Despite claims that English is important for economic and diplomatic reasons, my survey found that most Taiwanese did not support English education when it threatened local Taiwanese languages. The survey also found that despite a significant amount of debate in academic literature about the role of …