Digital Commons Network^™

Understanding The Host Immune Response Against Pseudomonas Aeruginosa To Develop Novel Therapeutics And Vaccines, Emel Sen Kilic

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pseudomonas aeruginosa is a Gram-negative opportunistic bacterium that causes a broad range of acute and chronic infections. The high adaptability and emergence of multidrug-resistant strains of this bacterium pose a significant threat to human health. Particularly, pneumonia caused by this pathogen is associated with high morbidity and mortality in immunocompromised patients. To prevent these infections, we aimed to develop novel vaccine strategies by characterizing the host immune response against P. aeruginosa. During respiratory infections, P. aeruginosa first contacts with epithelial cells along the respiratory tract. Using RNA-sequencing, we were able to characterize transcriptional changes of the epithelial cells in …

Haemonchus Contortus Antigen Identification And Use Of Tropomyosin In Ovine Vaccine Development, Brynnan P. Russ

Graduate Theses, Dissertations, and Problem Reports (ETD)

Globally, the small ruminant industry loses hundreds of millions of dollars due to parasitism by the gastrointestinal nematode Haemonchus contortus. This parasite feeds on blood in the host’s abomasum leading to disease including anemia, hypoproteinemia, lethargy, and death. There have been exhaustive efforts to manage this parasite including different management applications, anthelmintic treatment, and now integration of resistance genetics into a flock. Like antibiotics, overuse of anthelmintics has led to resistance of H. contortus to these drugs. Therefore, other methods of control are the main focuses of many research initiatives. St. Croix are a resistant breed of sheep that mount …

Examining The Use Of Mhealth Technology For Weight Management: A Hybrid Effectiveness-Implementation Trial In Family Medicine, Laurel A. Brabson M.S.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Overweight and obesity are prevalent and problematic conditions in the United States and worldwide, and effective weight management interventions are underutilized. Efforts to improve weight management practices have focused almost exclusively on changing physician behavior, without considering the larger healthcare context or the reciprocal patient-physician relationship. The current study explored the possibility of leveraging technology to improve the implementation of weight management clinical practice guidelines and increase patient-physician weight management discussions. 100 patients of five family medicine physicians were randomly assigned to either complete a weight management mobile application (app) prior to their primary care visit (app condition), or to …

Toward An Understanding Of The Professional Work Of Ncaa Division I Soccer Coaches, Lynda Ellen Bowers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Very little is known about the professional work of NCAA Division I (DI) coaches, which poses challenges for coach educators to create relevant professional development opportunities for coaches in this context. The present study looked at the full scope of work of NCAA DI FBS head women’s soccer coaches. The aim of this project was to explore the coaches’ perspectives of their jobs to improve our understanding of the contextual nuances of working in a DI environment. In this qualitative study, four participants were interviewed in-person utilizing a vignette-style, semi-structured interview guide. Data were analyzed using an interpretivist- constructivist approach …

The Immunological Response To Chronic Stress In Obese Mice, Matthew Corey Parsley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obesity and psychological stress are major risk factors for cardiovascular disease and have a positive correlation with vascular dysfunction. This functional deficit has been linked to a pro-inflammatory milieu, exacerbated by an upregulation of pro-inflammatory cytokines. Febuxostat, a xanthine oxidase inhibitor, has been shown to rescue vascular impairment in these models. The purpose of this study was to examine the individual and combined effects of obesity and chronic stress on neuroimmune cell populations, and assess if febuxostat is able mediate immune alterations.

Male mice (n=48) were assigned into either normal chow (lean) and high-fat diet (obese) groups. The lean animals …

Tree Growth And Water Use Efficiency During The Twentieth Century: From Global Trends To Local Drivers, Justin Michael Mathias

Graduate Theses, Dissertations, and Problem Reports (ETD)

The frequency and severity of extreme environmental conditions will continue to increase under global environmental change. How terrestrial plants respond to prolonged, and often novel environmental stressors, will have profound impacts on, and feedbacks with, the Earth climate system at local to continental scales. Central to these feedbacks are plant stomata, actively regulated pores on the leaves of plants that act as a control valve over the fluxes of carbon dioxide (CO₂) into the leaf during photosynthesis and water vapor (H₂O) out of the leaf during transpiration. Importantly, changes in stomatal aperture do not affect the …

Hepatitis B In At-Risk Groups: Three Studies To Guide Education, Testing, And Vaccination Efforts In West Virginia, Stacy Tressler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives: For over a decade, West Virginia’s (WV) rate of acute hepatitis B has been the highest in the United States (US). In 2017, WV’s rate was 11.7 per 100,000 population, almost 12 times higher than the national rate. The increase in acute hepatitis B cases is largely due to substance misuse, including injection drug use (IDU). Hepatitis B is a vaccine preventable disease yet many at-risk adults remain unvaccinated and susceptible to infection. The objectives of our studies are to 1) describe yearly changes in acute hepatitis B incidence in WV and assess county-level impact of the …

Impact Of Sire Selection And Breed On Parasite Resistance In Sheep, Andrew Ryan Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

Selection within and among breeds are strategies to mitigate the impact of parasitism given failing chemotherapeutics. While Texels have improved marketability compared to other parasite-resistant breeds, the mechanism by which Texels reduce fecal egg count (FEC) is unclear. The immune response to Haemonchus contortus (Hc) was compared in Texel, parasite-resistant St. Croix, and parasite-susceptible Suffolk sheep. Adult worms exposed to St. Croix- and Texel-derived peripheral blood mononuclear cells and serum in vitro had greater binding around the reproductive structures than Suffolk and worm egg release tended to be affected by breed (P = 0.09). Resistance in Texels …

Alternative Media And Mulches In Organic Vegetable Production, Heather R. Griffith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reducing or eliminating waste from organic vegetable production can conserve money as well as resources. Conventional greenhouse production of vegetable transplants often relies on abundant fertigation which produces large amounts of polluted runoff, and the field production of organic vegetables frequently relies on the use of polyethylene mulch. A greenhouse study was conducted to determine if organic transplants can be successfully produced in a greenhouse under reduced soil moisture in order to reduce leaching of nutrients and potential water pollution. To test this, we compared the effect of reduced irrigation volumes to the standard practice of irrigating with 120% of …

Resilience In The Mountains: Exploring The Labor And Motives Of Food-Caregiver Women Repairing Broken Food Systems In West Virginia Communities, Heidi Lynn Gum

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the past four years the Food Justice Lab, now housed within the Center for Resilient Communities at West Virginia University, hosted a series of food access planning workshops across the state of West Virginia. Mobilizing more than 200 participants, the Nourishing Networks workshop training program was designed to build grassroots capacity for food system change. Eighty-percent of workshop participants were women and dialogues recorded at these events revealed how women are disproportionately impacted by food insecurity and disproportionately labor to repair a broken food system. Women in West Virginia are not only growing food, feeding their families, selling it …

A Delphi Study Exploring The Impact And Feasibility Of Entrepreneurial Initiatives In College/University Kinesiology Programs, Cory Breithoff

Graduate Theses, Dissertations, and Problem Reports (ETD)

As public revenue sources dwindle, and society urges institutions to freeze their ever-increasing tuition rates, universities must be creative to increase their internal revenue. While kinesiology programs have begun to discuss such methods, they must also evaluate what is feasible and financially impactful. The purpose of this study was to gain a consensus of opinion regarding the impact and feasibility of entrepreneurial initiatives in higher education and to generate a list of facilitators and barriers that may impact the implementation. A total of 20 expert panelists comprised of higher education kinesiology administrators participated in a two-round Modified Delphi study to …

Evaluation Of Salmonella Enteritidis And Escherichia Coli O157:H7 Volatile Organic Compound Analysis For Food Safety Monitoring: A Preliminary Study, Jessica E. Lemley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Food borne illness has the potential to impact every point of production from producers to consumers. Food product recalls are common due to the presence of food borne illness causing bacteria. Detection of volatile organic compounds (VOC) have the potential to reduce the number of illnesses and recalls through application of additional monitoring of food products like those most commonly recalled. The objective of this research was to 1) explore the use of closed-loop headspace analysis coupled with gas chromatography-mass spectrometry (GC-MS) on food borne illness causing bacteria and 2) examine the VOC profile of Escherichia coli serotype O157:H7 in …

The State And War On Poverty: British Welfare Development And Its Legacies For Malawi, 1930s-1983, Gift Wasambo Kayira

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation documents the struggles and dilemmas that the Malawian state endured as it attempted to achieve its developmental goals from the 1930s to 1983. It contributes to histories of development by focusing on the interventions both the colonial and postcolonial states made to improve the living standards of African rural communities, the ideas which shaped state programs, and the behavior of the state which such interventions reveal. Scholars typically argue that state policy in Malawi was necessarily destructive and limited the economic progress of the local communities. The state deliberately pursued land, market, and other agricultural policies that constrained …

The Host Gatekeeper: Using The Flagellar Pathway To Understand Symbiont Host Adaptation, Adam R. Pollio

Graduate Theses, Dissertations, and Problem Reports (ETD)

The acquisition of microbial partners is a strategy used by a diverse group of arthropods to overcome ecological barriers that might normally make certain niches uninhabitable. The unique phylogenetic opportunities attainable from the natural experiment of the Sodalis-allied clade allow for better understanding of how molecular structures evolve through time. Here, we focus on the evolution of the flagellar synthesis pathway, due to its complexity and ability to diverge in response to ecological pressures. We used this molecular pathway and natural experiment to show that normal evolutionary outcomes associated with symbiosis (i.e., genome reduction) do not explain the predicted conservation …

Root Coverage Predictability In The Treatment Of Gingival Recessions On Mandibular And Anterior Teeth, Michele Agusto

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction. Mandibular anterior teeth are most frequently affected by gingival recessions. However, data regarding mucogingival treatment aimed at root coverage in this specific location is limited.

Aim. To systematically review the literature addressing the focused question: “What is the effectiveness, efficacy and efficiency of different surgical approaches on clinical and patient-related outcomes in the treatment of buccal gingival recessions on mandibular anterior teeth?”

Material methods. Electronic (Medline, Scopus, and Cochrane databases) and hand literature searches were performed for studies including at least one arm involving the use of pedicle flaps and/or free soft tissue grafts in the …

Allopurinol Alters The Expression Of Cytokines And Mediators Of Immune Cells In The Systemic 2 Tissue By Decreasing Plasma Urate., Lundrim S. Marku

Graduate Theses, Dissertations, and Problem Reports (ETD)

Diminished levels of urate have been linked to oxidative stress in birds and mammals. Urate, a major antioxidant that lowers reactive oxygen/nitrogen species (ROS/RNS), is thought to ameliorate the effects. The relationship between reduced urate, the immune system and the pathogenesis of the intestine, the liver or the brain has not been well characterized in avians. Moreover, recent studies have proposed that an increased permeability in the intestine due to some insult can induce inflammation in peripheral organs such as the brain. Allopurinol, a relatively toxic purine analogue that serves as a xanthine oxidase inhibitor, reduces urate levels which can …

A Video Decision Aid For The West Virginia Post: A Randomized, Controlled Trial, Jarred Vicente Gallegos

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Physician Orders for Life Sustaining Treatment (POLST) allows patients to express preferences for end-of-life medical decisions. Although POLST forms are used throughout the United States, evidence is lacking about the quality of the decision-making process of individuals completing these forms. The use of a decision aid developed for the POLST could ensure that patients completing these forms are informed, confident, and certain of their treatment decisions. The purpose of the study was to develop and evaluate a video decision aid for the West Virginia POST. 64 English-speaking, community-dwelling adults (50+), with no evidence of cognitive impairment, were recruited to …

Unbundling The “Big Deal”: One Library’S Experience In Rising To The Occasion, Susan J. Arnold, Traci J. Mays

Faculty & Staff Scholarship

Background:

Budgetary constraints at West Virginia University (WVU) in combination with the impending expiration/renewal of “big deal” contracts forced the WVU Collections Advisory Committee (CAC) to implement the unbundling of three major journal packages. Wiley was the first package unbundled in 2017, followed by Elsevier Science Direct and Springer packages in 2018-19.

Methods:

The CAC determined criteria to use to consider whether or not to keep specific journal titles, including total uses, cost per use, and number of papers published and references used from each title by WVU faculty members over a 3-year period. Journals were ranked for each of …

Childhood Trauma: An Analysis Of Trauma Severity, Symptoms, And Contributing Factors Within A Child Advocacy Center, Hannah Coffey

Munn Scholars Awards

Child abuse and neglect occur at high rates across the United States, with about one out of every seven children having experienced maltreatment in the past year (Centers for Disease Control and Prevention, 2018). Child maltreatment has been associated with a variety of negative outcomes such as developmental difficulties (Hildyard & Wolfe, 2002; Johnson et al., 2002), risky sexual behaviors (Harr et al., 2013; Haydon, Hussey, & Halpern, 2011; Maniglio, 2009; Thompson et al., 2016), criminal behavior (Edinburgh, Pape-Babolil, Harpin, & Saewyc, 2014; Higgins, Ricketts, Griffith, & Jirard, 2013; Makarios, 2007), long-term health problems (Maniglio, 2009; Trickett, Negriff, Ji, & …

Forbidden Flower: How Allowing Medical Cannabis Flower In West Virginia Helps Heal Patients And The State Economy, Jordan Maddy

West Virginia Law Review

No abstract provided.

Drought Management Before During And After The Drought, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

Drought Management Notes, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

Could Your Pasture Possibly Be Too Good., Edward Barrow Rayburn

Faculty & Staff Scholarship

Article discusses how high quality pasture can contain excessive amounts of protein that can reduce animal performance relative to the expected digestible energy in the forage.

Associations Of Adiposity And Diet Quality With Serum Ceramides In Middle-Aged Adults With Cardiovascular Risk Factors, Margaret A. Drazba, Ida Holaskova, Nadine R. Sahyoun, Melissa Ventura Marra

Faculty & Staff Scholarship

Rates of adverse cardiovascular events have increased among middle-aged adults. Elevated ceramides have been proposed as a risk factor for cardiovascular events. Diet quality and weight status are inversely associated with several traditional risk factors; however, the relationship to ceramides is less clear. This study aimed to determine associations of adiposity and diet quality with circulating ceramides in middle-aged adults (n = 96). Diet quality was estimated using the Healthy Eating Index 2015 (HEI-2015). Serum ceramide concentrations were determined by liquid chromatography–mass spectrometry. A ceramide risk score was determined based on ceramides C16:0, C18:0, and C24:1 and their ratios to …

Sex Differences In Lifestyle Behaviors Among U.S. College Freshmen, Melissa D. Olfert, Makenzie L. Barr, Camille C. Charlier, Geoffrey W. Greene, Wenjun Zhou, Sarah E. Colby

Clinical and Translational Science Institute

Within lifestyle behavior research, the sex of populations causes differences in behaviors and outcomes of studies. This cross-sectional study investigated lifestyle behavior patterns in college students, examining sex differences in four areas: Nutrition, physical activity, sleep, and stress. Data from over 1100 college freshmen across 8 United States universities were used for this cross-sectional analysis. Self-reported data assessed fruit and vegetable intake, fat percent intake, physical activity, perceived stress, and sleep quality. Statistical analysis included Pearson chi-squared and Mann–Whitney’s U tests for scores by sex. Likewise, healthy cut-offs were used to determine frequency of participants within range of the five …

Association Between Breastfeeding And Childhood Cardiovascular Disease Risk Factors, Amna Umer, Candice Hamilton, Roger A. Edwards, Lesley Cottrell, Peter Giacobbi Jr., Kim Innes, Collin John, George A. Kelley, William Neal, Christa Lilly

Clinical and Translational Science Institute

Introduction The immediate benefits of breastfeeding are well-established but the long-term health benefits are less wellknown. West Virginia (WV) has a higher prevalence of cardiovascular disease (CVD) and lower breastfeeding rates compared to national averages. There is a paucity of research examining the relationship between breastfeeding and subsequent childhood CVD risk factors, an issue of particular relevance in WV. Methods This study used longitudinally linked data from three cross-sectional datasets in WV (N=11,980). The information on breastfeeding was obtained retrospectively via parental recall when the child was in the fifth grade. The outcome variables included blood pressure measures [systolic blood …

Alkaline Phosphatase: A Potential Biomarker For Stroke And Implications For Treatment, A L. Brichacek, C M. Brown

Clinical and Translational Science Institute

Stroke is the fifth leading cause of death in the U.S., with more than 100,000 deaths annually. There are a multitude of risks associated with stroke, including aging, cardiovascular disease, hypertension, Alzheimer’s disease (AD), and immune suppression. One of the many challenges, which has so far proven to be unsuccessful, is the identification of a cost-effective diagnostic or prognostic biomarker for stroke. Alkaline phosphatase (AP), an enzyme first discovered in the 1920s, has been evaluated as a potential biomarker in many disorders, including many of the co-morbidities associated with stroke. This review will examine the basic biology of AP, and …

Monetary Cost Of The Myplate Diet In Young Adults: Higher Expenses Associated With Increased Fruit And Vegetable Consumption, Rashel L. Clark, Oluremi A. Famodu, Makenzie L. Barr, Rebecca L. Hagedorn, Jane Ruseski, Jade A. White, Caitlin M. Warner, Alexandra M. Morrell, Pamela J. Murray, I Mark Olfert, Joseph W. Mcfadden, Marianne T. Downes, Sarah E. Colby, Melissa D. Olfert

Faculty & Staff Scholarship

Background. Cost is a commonly reported barrier to healthy eating. This is a secondary research analysis designed to examine the food expenditures of young adults on a university campus following the United States Department of Agriculture (USDA) MyPlate guidelines for fruits and vegetables. Methods. Meal receipts and dietary intake were recorded weekly. Anthropometrics and clinical assessments were recorded before intervention. Researchers rated compliance based on the participant’s dietary food log, receipt matching, food pictures, and reports during weekly 1-hour consultations. Results. Fifty-three young adults (18–30 years old) at-risk of, or diagnosed with, metabolic syndrome (MetS) were enrolled in the study, …

Educational Intervention Improves Fruit And Vegetable Intake In Young Adults With Metabolic Syndrome Components, Rashel L. Clark, Oluremi A. Famodu, Ida Holásková, Aniello M. Infante, Pamela J. Murray, I. Mark Olfert, Joseeph W. Mcfadden, Marianne T. Downes, Paul D. Chantler, Matthew W. Duespohl, Christopher F. Cuff, Melissa D. Olfert

Faculty & Staff Scholarship

The FRUVEDomics study investigates the effect of a diet intervention focused on increasing fruit and vegetable intake on the gut microbiome and cardiovascular health of young adults with/at risk for metabolic syndrome(MetS). It was hypothesized that the recommended diet would result in metabolic and gut microbiome changes. The 9-week dietary intervention adhered to the US Department of Agriculture Dietary Guidelines for Americansand focused on increasing fruit and vegetable intake to equal half of the diet. Seventeen eligible young adults with/or at high risk of MetS consented and completed preintervention and postintervention measurements, including anthropometric, body composition, cardiovascular, …

2019 Symposium Brochure

Programs

No abstract provided.