Digital Commons Network^™

Exposure Therapy: Stimulus Intensity As A Factor In Treatment Preference, Matthew C. Arias

Graduate Theses, Dissertations, and Problem Reports (ETD)

Currently, a host of treatments are available for treating anxiety disorders, including specific phobia. Treatment of specific phobia includes pharmacological, psychosocial, and combined approaches. Exposure therapy, however, is considered the leading psychological treatment for specific phobia, and has shown to be effective. Exposure therapy challenges held beliefs about feared stimuli/situations and attempts to integrate new learning about the feared object/situation. Despite exposure being effective to treat specific phobia, it is associated with low adherence and high dropout rates. There is a need to examine, therefore, ways to improve patient adherence for exposure. A way to examine this issue is to …

Population Genetics Of A Reintroduced Fisher (Pekania Pennanti) Population In West Virginia, Caroline E. Harms

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fishers (Pekania pennanti) were reintroduced in West Virginia in 1969 with no population wide genetic analysis ever having been conducted. Genetic analysis of reintroduced populations is vital to assess genetic diversity as an indicative of population viability. My objective was to collect fisher samples throughout West Virginia, nearby states where fisher may be migrating (PA and NY), and the New Hampshire source population to provide a full genetic interpretation of the reintroduced West Virginia population. Genetic analysis was used to measure genetic diversity, admixture or delineation of subpopulations, and effective population size. Sample location and genetic data were …

Unconventional Gas Pipeline Right-Of-Way Influence On Wildlife, Samuel C. Knopka

Graduate Theses, Dissertations, and Problem Reports (ETD)

The development of horizontal drilling for natural gas in the Marcellus Shale in 2004 resulted in rapid exploitation of the play in the Appalachian Basin. This “unconventional” gas well drilling accesses resources deeper beneath the surface and allows for multiple wells to be co-located on a single well pad. This results in fewer, but larger, well pads on the landscape than traditional vertical wells. Pipelines are required to transport the volumes of gas produced by unconventional wells to production facilities and market. The effects of gathering pipelines, which transport gas from well pads to larger transport pipelines, are poorly studied …

Various Operational Aspects Of The Extraction Of Critical Minerals From Acid Mine Drainage And Its Treatment By-Product, Zhongqing Xiao

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid mine drainage (AMD) is environmentally hazardous acidic water, which is continuously generated in large volumes at coal mining sites, typically when pyrite is exposed to oxygen and water. As its continuous migration under the geologic conditions, AMD tends to leach metal elements out from the surrounding minerals, leading to high concentrations of metal elements, like iron, aluminum, magnesium, manganese, lead, arsenic, as well as critical cobalt and rare earth elements (REEs). AMD must be treated and monitored to meet the effluent limits, under U.S.C §1251, the Clean Water act, before being discharged to receiving streams.

Recently, the research is …

Examining The Effects Of Background Noise On Contextualized Word Learning, Caitlin Alyssa Ross

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite redundancy in the acoustic speech signal, both children and adults demonstrate difficulty listening and learning in noise. Research has shown that the acoustic parameters of classrooms and common study places, such as libraries and coffee shops, are often exposing students daily to unhealthy levels of background noise and distraction as they attempt to access and retain new information. While younger children may encounter new words via deliberate instruction in a classroom setting, older students are more likely to access new vocabulary indirectly via reading or self-study in noisy environments often of their own choosing. In both scenarios, accurate perception …

A Survey Of Anthelmintic Resistance In Angus Bulls Participating In Centralized Performance Tests In Appalachia, Roger Tyler Rohrbaugh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Resistance to anthelmintics, more commonly referred to as “de-wormers,” has been on the rise in parasites that affect cattle. Parasite infection can have severe impacts on calf productivity resulting in reduced profitability. Therefore, we sought to better understand anthelmintic resistance in Appalachia by surveying Angus bulls entering performance tests in VA and WV. During a four-year period, over 1050 Angus bulls were tested at bull development facilities in Wardensville, WV, Culpeper, VA and Wytheville, VA. Fecal samples were collected at arrival and 14-21 days later for determination of fecal egg count (FEC) reduction. Upon arrival, all bulls received either fenbendazole …

Effect Of Diet, Age, And Genetics On Rainbow Trout Growth And Fillet Quality, Bahareh Adami

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study, analyses were conducted to compare fillet quality attributes of 4 rainbow trout families (40, 102, 159, and 187) fed fish meal (FM) and a fishmeal free diet (FMF), to two age endpoints, 9 and 11 months.

Previous studies with rainbow trout have evaluated the effect of age, diet, and family on growth performance and fillet quality. However, a comprehensive analysis of variables is lacking for rainbow trout, one of the main aquatic foods for domestic and international consumers.

Separable muscle as a percent whole body weight was not affected by diet, family, age, and the interaction of …

Mau Mau’S Moral War: The Sacralization Of Kenya’S Anticolonial Struggle, 1952–1956, Henry Muoki Mbunga

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is a study of the ethical and religious history of the Mau Mau War in Kenya, 1952–1956. It breaks with a historiographical tradition that reduces Mau Mau’s ritual activities to stage props and backdrops on the historical stage. The project investigates two central questions: (1) why did Mau Mau members invent a variety of rituals during the anticolonial struggle in Kenya? and (2) how did the ethical and religious convictions of Mau Mau members shape their military campaign? Using rare archival sources and the oral histories and published memoirs of Mau Mau War veterans, this study argues that …

Standby Lovers: A Typology And Theoretical Investigation Of Back Burner Relational Maintenance, Dana Borzea

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this dissertation was to investigate the connections between relational maintenance behaviors, theoretical factors, and relational characteristics in back burner relationships. A back burner relationship involves at least one individual who is romantically or sexually interested in a target, but they are not currently involved with the target. Given that back burners maintain communication with each other with the possibility of becoming romantically or sexually involved in the future, Study 1 was concerned with inductively identifying the relational maintenance behaviors used in back burner relationships. Following prior typology methods, participants (N = 86) in Study 1 were …

Describing A Putative Corollary Discharge Circuit In Drosophila, Kaitlyn Nicole Boone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Corollary discharge (CD) circuits provide critical information about movement and behavior to provide context to sensory processing. However, to date, there has not been a comprehensive study of CD circuits at a single-cell level. In this thesis, I aimed to resolve the connectivity of ascending histaminergic neurons, consisting of two pairs, the mesothoracic pair (MsAHNs) and metathoracic pair (MtAHNs) at a single-cell level and characterize contexts of activation. Using transgenic techniques, connectomics and transcriptomics, we identify neural populations receiving input from the AHNs and neural populations with significant output to the AHNs. We explored where the AHNs predominantly receive synaptic …

Ecology Of Freshwater Turtles And Other Wetland Wildlife In A North-Central West Virginia Watershed, Darien N. Lozon

Graduate Theses, Dissertations, and Problem Reports (ETD)

The goal of wetland mitigation in the United States is to achieve a no-net-loss of wetlands; however, mitigated wetlands must be monitored to ensure wetland function is comparable to natural wetlands. In this study, relationships between land use practices and freshwater turtle abundance, wetland connectivity, and heavy metal bioaccumulation were investigated. In Chapter 1, a summary of pre-restoration species abundance and diversity is provided for anurans, birds, benthic macroinvertebrates, fishes, small mammals, plants, and turtles in riparian wetlands along Ruby Run, a tributary of Deckers Creek in north-central West Virginia, USA. These data provide a baseline for comparison after mitigation …

Exploring The Perceptions Of Produce Processors Operating In Non-Profit Commercial Kitchens In West Virginia, Megan Christine Govindan

Graduate Theses, Dissertations, and Problem Reports (ETD)

According to the Centers for Disease Control and Prevention, 48 million people get sick, 128,000 are hospitalized and 3,000 die each year from foodborne illness. The Food Safety Modernization Act (FSMA) shifted the focus of the Food and Drug Administration from response, to prevention of foodborne illness. The FSMA identified seven rules related to food safety prevention measures, including inspection and compliance, response and enhanced partnerships to ensure food safety along the food system, and employee-training compliance measures. Increasing access to healthy, local foods has economic, public health, and environmental benefits. Farm to Institution policies are becoming more popular nationally, …

Manipulating The Perineuronal Net In The Deep Cerebellar Nucleus, Deidre E. O'Dell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Perineuronal nets (PNN) are a type of specialized extracellular matrix in the central nervous system. The PNN forms during postnatal development but the ontogeny of the PNN has yet to be elucidated. Studying the PNN in the rat brain may allow us to further understand the PNN’s role in development, learning, and memory. The PNN is fully developed in the deep cerebellar nuclei (DCN) of rats by post-natal day 18. By using enzymatic digestion of the PNN with chondroitinase ABC (ChABC), we studied how digestion of the PNN affects cerebellar-dependent eyeblink conditioning (EBC) and performed electrophysiological recordings from DCN neurons. …

Weed Recognition In Agriculture: A Mask R-Cnn Approach, Sruthi Keerthi Valicharla

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent interdisciplinary collaboration on deep learning has led to a growing interest in its application in the agriculture domain. Weed control and management are some of the crucial tasks in agriculture to maintain high crop productivity. The inception phase of weed control and management is to successfully recognize the weed plants, followed by providing a suitable management plan. Due to the complexities in agriculture images, such as similar colour and texture, we need to incorporate a deep neural network that uses pixel-wise grouping for identifying the plant species. In this thesis, we analysed the performance of one of the most …

Initial And Advanced Stages Of Microbiota Establishment Within The Tsetse Fly, Miguel Eduardo Medina Munoz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Symbiosis is a long-term physical association between two or more species, although little is known regarding its evolutionary origins, particularly at the genetic level. Tsetse flies are the vector of African trypanosomes, causative agents of Human and Animal African Trypanosomiases. Tsetse provide an ideal model for studying initial and advanced stages of symbiosis. Tsetse have a simple digestive tract microbiota primarily consisting of two bacteria; the ancient mutualist Wigglesworthia glossinidia and the recently acquired Sodalis glossinidius. This work presents a chronological study in evolutionary terms of the history of a microbial-insect association. First, I present concepts on symbiosis and …

Bypassing The Blood-Brain Barrier: A Physical And Pharmacological Approach For The Treatment Of Metastatic Brain Tumors, Samuel A. Sprowls

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation (a) provided an in depth literature review of methods to disrupt the BBB/BTB and improve therapeutic distribution to brain tumors, (b) evaluated the use of azacitidine as a single agent therapy for the treatment of brain metastasis of breast cancer and a potential molecular mechanism by which brain tropic cells are sensitized to hypomethylating agents, (c) determined the impact cannabidiol has on P-glycoprotein mediated efflux at the blood-brain barrier and its potential for use as a single agent treatment for metastatic brain tumors, (d) developed a preclinical radiation therapy protocol for use in small animals and in vitro …

Evaluation Of Population Structure, Age, Growth, And Mortality Of Blue Catfish And Flathead Catfish Within The Robert C. Byrd Pool Of The Ohio And Kanawha Rivers, Joseph Vincent Siegel

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Ohio River is continuing to recover from a long history of anthropogenic impacts. Blue Catfish were considered locally extirpated from the West Virginia sections and have been reintroduced through fingerling stockings beginning in 2002. Flathead Catfish are naturally sustaining in the pool and combined with Blue Catfish provide two popular recreational fisheries. The Blue Catfish and Flathead Catfish studies shared similar objectives: (1) evaluating size structure, (2) estimating growth rates, and (3) estimating annual mortality. From 2017 to 2020, we collected catfish annually through low frequency electrofishing and trotline surveys at 10 fixed locations. Electrofishing catch rates for Blue …

The Receptor Basis Of Serotonergic Modulation In An Olfactory Network, Tyler Ryan Sizemore

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neuromodulation is a nearly ubiquitous process that endows the nervous system with the capacity to alter neural function at every level (synaptic, circuit, network, etc.) without necessarily adding new neurons. Through the actions of neuromodulators, the existing neural circuitry can be adaptively tuned to achieve flexible network output and similarly dynamic behavioral output. However, despite their near ubiquity in all sensory modalities, the mechanisms underlying neuromodulation of sensory processing remain poorly understood. In this dissertation, I address three main questions regarding the mechanisms of one modulator (serotonin) within one sensory modality (olfaction). I begin by establishing a "functional atlas" of …

Effect Of Fecal Egg Count Estimated Breeding Value On Antibody Production In Katahdin Lambs, Kelsey L. Bentley

Graduate Theses, Dissertations, and Problem Reports (ETD)

With current pressures to seek out genetic solutions to combat antimicrobial resistance, genetic resistance to the gastrointestinal nematode Haemonchus contortus may reduce need for prophylactic antibiotic use. The Katahdin breed has effectively utilized post-weaning fecal egg count estimated breeding value (PFEC EBV) to reduce parasitism within their flocks, but there still remains significant opportunity for selection within breed. In a previous study, Katahdin sheep sired by low PFEC sires had greater survivability to weaning than lambs sired by high PFEC rams and preweaning was caused by other diseases than parasitism. Those data lead to the hypothesis that selection for PFEC …

Tree Species Influence On Microbial Functional Gene Abundance And N Cycling In Riparian Soil, Elizabeth Matejczyk

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about how above-ground vegetation may differentially influence the below-ground microbial community structure, abundance, and function. Abundance of soil microbial N cycling genes responsible for nitrification (amoA), denitrification (nirK and nirS), and nitrous oxide reduction (nosZ) may vary with tree species and increasing N availability, and these variables may be used to predict production of NO₃^- and N₂O from soil. Variability of nitrification and denitrification rates have also been linked to tree mycorrhizal associations, with soil beneath tree species associated with arbuscular mycorrhizal (AM) exhibiting greater denitrification rates …

Women, Water, And Well-Being: Gendered Experiences Of Household Water Contamination In Parkersburg, West Virginia, Emily Brooke Tingler

Graduate Theses, Dissertations, and Problem Reports (ETD)

DuPont Washington Works chemical plant knowingly pumped hundreds of thousands of pounds of toxic C8 waste into local waterways, water tables, and landfills for over 40 years, contaminating the drinking water for more than 100,000 residents in and around Parkersburg, West Virginia. Drawing on feminist political ecology and the political ecology of water with literature on perceptions of risk in contaminated communities, environmental reproductive justice, modern water, and hydrosocial waterscapes, I examine, through a qualitative case study, the lived experiences of women who have an intimate understanding of C8 water contamination from the DuPont Washington Works chemical plant. Specifically, I …

The Effects Of Genetic Differences In An Innate Immune Receptor On Immune Responses To Haemonchus Contortus In Sheep, Denzel Deshawn Middleton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 …

Timber Bridge Pile Splicing With Fiber Reinforced Polymer Wraps, Drew L. Damich

Graduate Theses, Dissertations, and Problem Reports (ETD)

Timber pile repair using splicing is widely used but little research has been done to determine their strength capacity after repair using this method. Current timber pile splicing mechanisms utilize various steel or wooden components. Fiber Reinforced Polymer (FRP) wraps can be utilized as replacement to conventional materials in splicing of timber piles. This study evaluated the strength capacities of traditional splicing mechanisms in relation to FRP wrap splice mechanisms. Traditional splicing mechanisms consisted of flat steel plate, C-channel steel plate, and wooden plate splices. The FRP wrap splice consisted of unidirectional glass/epoxy composite with three layers of fabric as …

Examining West Virginia's Economic Development: Natural Resources, Development Agencies, And Labor Force Development, Gaillynn Marie Bowman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Examining West Virginia's Economic Development: Natural Resources, Development Agencies, and Labor Force Development Gaillynn Bowman Economic development is well-recognized as being fundamental to facilitating an overall improved quality of life for communities and their residents. Throughout West Virginia’s history, the state has experienced economic hardships caused by boom and bust cycles associated with resource extraction. This dissertation consists of three essays that explore the impacts of economic development activities, including conservation programs, economic development agency initiatives, and workforce development agencies. The first essay explores the relationship nonresident landowners have with the conservation programs within West Virginia, specifically the Natural Resources …

Novel Mechanisms Of Neuronal And Hormonal Regulation Of Large Intestinal Electrolyte Transport, Andrew John Nickerson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The mammalian colon plays a vital role in maintaining overall electrolyte and fluid homeostasis in the body, and it possesses the ability to either secrete or absorb water depending upon the needs of the organism. Secretion or absorption of electrolyte ions, such as Na⁺, Cl^- and K⁺, drives the movement of water across the colonic epithelium – the inner gut lining where all transport takes place. Aberrant absorptive and/or secretory activity within the colonic epithelium is central to the pathogenesis of many gastrointestinal diseases, such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), constipation …

Pandemic Pedagogy: A Zoom Teaching Experiment Using Cognitive Theory Of Multimedia Learning Principles Of Multimedia Design, Kevin C. Knoster

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this dissertation was to conduct an experimental study exploring the applicability of multimedia principles of effective instructional design to Zoom teaching. In the wake of the COVID-19 pandemic, educators across higher education were forced to rapidly transition from traditional face-to-face instruction to online teaching. One of the most common ways in which colleges and universities navigated this transition in the United States was via mass adoption of the video conferencing platform Zoom. However, best practices have not yet been identified to assist instructors inexperienced with online teaching in adapting to remote instruction via Zoom. This dissertation argued …

Targeting The Cerebrovasculature In Sepsis: A Focus On The Brain Microvascular Endothelium, Divine C. Nwafor

Graduate Theses, Dissertations, and Problem Reports (ETD)

The blood-brain barrier (BBB) is a critical interface between the systemic circulation and the brain. It is a specialized multicellular unit composed of brain microvascular endothelial cells (BMECs), pericytes, a basement membrane, and astrocytic end foot processes. BMECs are a principal component of the BBB that provide the structural framework needed for the stringent transport of molecules into the brain. BMEC dysfunction permits the trafficking of neurotoxins from systemic circulation into the brain, which ultimately exacerbates BBB dysfunction and neuroinflammation. Studies have shown that BBB dysfunction is a key determinant of cognitive decline in sepsis. However, there are critical knowledge …

Seriously Doe: Why A Hybrid Approach To Regulating Deer Farms Is Right For West Virginia, Jordan R. Mcminn

West Virginia Law Review

No abstract provided.

China’S Illegal Organ Trade: From Executed Prisoners To Organ Tourism To Falun Gong, Adrienne Thompson

West Virginia University Historical Review

As worldwide populations continue to rise, the constant necessity for life-saving organs for terminally and chronically ill patients has become extremely vital and profitable to medical centers across the globe. Since China’s preliminary debut in the international organ donation and transplantation system in the late 1960s, various scholars, journalists, and health professionals across the globe have demonstrated outright shock at the massive influx and seemingly endless supply of transferable organs emanating from Chinese transplant centers and hospitals. Investigations have shown that China has been actively harvesting organs from recently executed prisoners and incarcerated Falun Gong practitioners. Regardless of numerous outside …

Japanese Economic Aggression, Organized Labor’S Resistance, And The Path To World War Ii, Thomas Miles

West Virginia University Historical Review

This paper examines economic aggression from the Japanese fishing fleet beginning in 1936 and the response from organized labor in America prior to the famous attack on Pearl Harbor. The focus of this is research is primarily from the perspective of American labor, drawing heavily on the Voice of the Federation newspaper, which was a publication owned by the Maritime Federation of the Pacific. The US government was aware of encroachment of Japanese floating canneries in 1936, but took little action against Japan in order to avoid worsening tensions between the two nations. However, in 1937 Harry Stuhr, the head …