Exploring The Usage Of Acid Mine Drainage Sludge As A Soil Amendment For Reclaimed Mine Lands, Brady Watters

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid mine drainage (AMD) is a form of water pollution generated when water and oxygen come into contact with sulfide minerals, forming metal ions, sulfuric acid, and sulphates in solution. AMD is characterized by its toxic metals content and low pH, both of which are an environmental concern. The treatment of AMD separates the contaminants into a high-water content sludge. This sludge is a pure waste product under current evaluation for alternative uses as well as improvements to storage and treatment processes. This thesis focused on analyzing the potential for AMD sludge to be used in recently reclaimed lands as …

Gender Inclusivity In Italian: Can Gender Neutrality Be Reached In A Gendered Language? Difficulties, Proposals And Public Perception Of The Phenomenon, Gaia Prunotto

Graduate Theses, Dissertations, and Problem Reports (ETD)

Italian is a gendered language in which reference to grammatical gender has been constant and inevitable. As language reflects and shapes society, grammatical gender reinforces binary views of social gender and the invizibilization of non-binary identities. Movements for social justice have inspired gender-fair linguistic innovations, such as the feminization of job titles and gender-neutral markers, to promote gender equity and challenge hierarchies of power and dominance in society. This research aimed to explore gender-neutral forms in the Italian grammatical system and public perceptions of these innovations through two studies with two groups of participants.

The first group of participants (n=27) …

Effects Of Invasive Species On Ohio River Zooplankton, Samuel John Johnston

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species have the potential to dramatically change both aquatic and terrestrial ecosystems with rates of introduction only expected to increase across the Globe. In the Mississippi River basin filter feeding silver (Hypophthalmichthys molitrix) and bighead carp (Hypophthalmichthys nobilis) collectively known as invasive carp were introduced into aquaculture ponds where they subsequently escaped and spread to interconnected basins. Invasive carp have not yet completely established throughout the entire Ohio River. Managers recognize three distinct phases of carp invasion: establishment front, invasion front and presence front. In the Illinois River invasive carp are very well established and …

Wvu Core Arboretum User Motivations, Demographics, And Satisfaction: A Snapshot From Summer Of 2022 To Spring Of 2023, Bennett F. Phillips

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper presents a comprehensive examination of user motivations, demographics, and satisfaction levels at the West Virginia University (WVU) Core Arboretum during the period spanning from the summer of 2022 to the spring of 2023. Green spaces across the United States have seen an increase in public attention in the wake of the COVID-19 pandemic and are actively seeking to capitalize on the renewed interest. Seeking to aid the WVU Core Arboretum in garnering renewed public interest, this study utilized a survey comprising sections on user perceptions, demographics, satisfaction, and usage that garnered 182 responses.

Demographic analysis of the survey …

Longitudinal Oxygen Imaging In 3d (Bio)Printed Models, Ryan Curtis O'Connell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electron paramagnetic resonance (EPR), and its molecular imaging modality, is a powerful tool to noninvasively map various biological and chemical markers within objects of interest. Reliable data acquisition is a major impeding factor for longitudinal hands-off measurements. Measurements are especially challenging in biomedical applications, as live objects are not static. Frequent changes occur that require constant fine recalibration of the EPR detection system, called the resonator. To enable longitudinal imaging, a technology permitting automatic digital control of resonator coupling, tuning, and EPR data acquisition was developed. Automation was achieved through the utilization of a microcontroller and digital peripheral components such …

Investigation Of Regulator Of G Protein Signaling-12 (Rgs12) As A Modulator Of Monoamine Transporters And Behavioral Responses To Cocaine, Allison Noel White

Graduate Theses, Dissertations, and Problem Reports (ETD)

Stimulant use disorder is a psychiatric illness that is characterized by repeated use of stimulant drugs despite harm to the user. There are currently no Federal Drug Administration (FDA)- approved medications for stimulant use disorder, and the number of stimulant use-associated deaths have increased over the past decade. Given the prevalence of and lack of treatments for stimulant use disorder, there is an urgent need to identify new drug targets and treatments for this illness. Our group is investigating Regulator of G protein Signaling-12 (RGS12) as one such potential drug target for stimulant use disorder. Mice lacking RGS12 are insensitive …

Krill Protein Fortified Extrudates: A Pilot Study, Avery S. Fleeharty

Graduate Theses, Dissertations, and Problem Reports (ETD)

Antarctic krill (Euphausiacea superba) is an abundant and sustainable but underutilized natural resource with well-studied protein isolation methodology using Isoelectric solubilization-precipitation (ISP). The resulting krill protein isolate (KPI) has high yield and sufficient impurity removal in addition to desirable nutritional and functional properties but has yet to be used in food systems. Extrusion is a versatile and scalable food processing method with many benefits; thus, the aim of this study was to determine the impact of KPI as an additive in extrusion processing with the eventual goal of introducing it to human and animal food systems. KPI was …

Evaluating Innovative Methods Of Quantitatively Linking Microbial Community Structure To Ecosystem Function, Jeth Gv Walkup

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microbial functional diversity is the product of community structure and intraspecific trait variation. Due to microbial diversity and limited availability of microbial trait measurements, it has been challenging to quantitatively link community structure to microbial function. Although community-level activity rates vary with community composition, issues in the scale of their measurement inhibit our understanding of their relationship. Quantitative stable isotope probing (qSIP) is a method for quantitatively measuring taxon-specific microbial traits, providing new opportunities to apply trait-based approaches for studying microbial ecology. Quantitative microbial trait data from these experiments enables the functional characterization of microbial communities, potentially enabling the extrapolation …

Outdoor Recreation Participation: Negotiation Strategies For Overcoming Constraints Related To Race, Ethnicity And Gender In Florida Keys National Marine Sanctuary, Larissa Martins

Graduate Theses, Dissertations, and Problem Reports (ETD)

By understanding the demographics of those participating in outdoor recreation, and identifying who is using these resources, managers can effectively create opportunities for underrepresented communities. Previous research had underscored the innumerous benefits related to coastal outdoor recreation activities, from human well-being to local socio-economic benefits. This study is divided into two papers that both took place in Florida Keys National Marine Sanctuary. The purpose of the first paper was to investigate the constraints that minority groups related to gender, race and ethnicity groups face prior participation in coastal activities, along with negotiation strategies that are employed in order to overcome …

Adventures In Flute Playing: A Literature Survey And Anticipated Beginning Flute Method, Sammy Holloman

Graduate Theses, Dissertations, and Problem Reports (ETD)

This Literature Survey will investigate 10 method books used by beginning flute students, aged 6-13, in the United States. In this survey, books will be analyzed to identify which musical concepts are covered and their overall methodical approach. Comprehensive graphs are offered in the Appendix to provide readers brief contextual information on the books analyzed.

The results from this survey have influenced the creation of my beginning flute method, Adventures in Flute Playing, which incorporates exercises that are less commonly found in the method books being used today. Adventures in Flute Playing will consist of a series of online videos …

Methods For Analyzing Physics Student Retention And Physics Curricula, John Darrell Hansen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Retention of students in college has been a concern of academic institutions for many years. In the last two decades, the focus on student retention in STEM fields has intensified. The current graduation rate of students in science, technology, engineering and mathematics (STEM) fields is well below that required to fill the projected need of STEM professionals. The work presented in this dissertation investigates the problem of student retention in physics programs. Four studies were performed. The first identifies the relationships between student retention and pre-college and early-college academic factors at an eastern U.S. university using logistic regression and Bayesian …

Localization Of People In Gnss-Denied Environments Using Neural-Inertial Prediction And Kalman Filter Correction, Lauren N. Cash

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis presents a method based on neural networks and Kalman filters for estimating the position of a person carrying a mobile device (i.e., cell phone or tablet) that can communicate with static UWB sensors or is carried in an environment with known landmark positions. This device is used to collect and share inertial measurement unit (IMU) information — which includes data from sensors such as accelerometers, gyroscopes, and magnetometers — and UWB and landmark information. The collected data, in combination with other necessary initial condition information, is input into a pre-trained deep neural network (DNN) which predicts the movement …

Physical Activity Promotion, Assessment, And Engagement In Clinical Settings In The United States, Kristin A. Grogg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Physical inactivity is an important contributor to morbidity and all-cause mortality and the 2018 Physical Activity Guidelines Advisory Committee recommended that physicians increase their role in physical activity assessment and promotion to combat physical inactivity and related comorbidities. Healthcare providers are increasingly called upon to initiate physical activity promotion with their patients to manage conditions like obesity, diabetes, and cardiovascular diseases. Still, recent reports indicate that less than half of primary care visits include some type of physical activity promotion. Although the National Physical Activity Plan includes some recommendations for clinicians and the healthcare sector on physical activity promotion, it …

Exogenous Factors That Impact Huntingtin Aggregation, Adam Skeens

Graduate Theses, Dissertations, and Problem Reports (ETD)

While expansion of a polyglutamine (polyQ) domain is the immediate cause of huntingtin (htt) aggregation associated with Huntington’s Disease (HD), other cellular factors modify aggregation. These include interactions with cellular membranes, protein biding partners, molecular crowding, and proteinaceous seeds. Here, two important factors are biophysically characterized: 1) the interaction of htt with endomembranes and 2) proteinaceous seeds obtained from a variety of htt-derived peptides. In the first project, the aggregation of htt at bilayer interfaces and in the presence of divalent cations was investigated. A major cellular factor implicated in altered htt aggregation is the binding of lipids. Furthermore, the …

Art-Based Social And Emotional Learning Curriculum In The K-6 Classroom: A Mixed Methods Study, Michelle Fegeley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Preparing elementary school students to succeed in a learning environment that was significantly transformed by a global pandemic will require a major investment in new curricula that focuses more on social and emotional learning and less on standards that primarily emphasize just knowing and doing. This research investigates how the implementation of social emotional learning and SEL-based art activities in the art classroom can help to lower student stress levels in the elementary school art classroom. By providing learners with an opportunity to grow socially and emotionally, teachers may help students understand their feelings and use artmaking as a way …

Using Aromatherapy For Comfort, Ease, And Stress For Adults Being Treated For Substance Use Disorder In North Central Appalachia: A Randomized Controlled Trial, Marian E. Reven

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background:Those in treatment for substance use disorder (SUD) face significant challenges and express the need for enhanced support for comfort to be successful. There is growing interest in integrative modalities such as aromatherapy using essential oils to support health.

Purpose: The purpose of this study was two-fold. First, to see if there was an increase in comfort and ease, and a decrease in stress in those who used an aroma inhaler with Citrus bergamia (Bergamot) essential oil. Second, to determine the feasibility of the intervention.

Method: This study was founded upon an integrated theoretical framework combining the theory of …

Feasibility Of Applying Motion Magnification In Subsurface Defect Detection For Concrete And Fiber-Reinforced Polymer Specimens, Nagavardhani Malineni

Graduate Theses, Dissertations, and Problem Reports (ETD)

Irrespective of size and complexity, every civil infrastructure needs certain scrutiny regarding its structural health to ensure its serviceability during its lifetime. In olden times such scrutiny was done with the aid of destructive testing methods using sensors that required cumbersome and expensive installations and led to the destruction of at least part of the tested specimen. However, in recent times, many non-destructive methods, such as acoustic emission testing, electromagnetic testing, and laser testing methods have emerged, leaving the specified tested specimen undisturbed. With the advancement in sensor technology like motion magnification and with the help of access to high-speed …

Essays On The Economics Of Law And Crime, Zachary J. Porreca

Graduate Theses, Dissertations, and Problem Reports (ETD)

The first chapter examines the connection between gentrification and urban violence. I demonstrate a positive and plausibly causal relationship between urban redevelopment and gun violence in Philadelphia. As the underlying mechanism, I focus on gentrification's displacement effect on local drug markets. Treating the city as a spatial network of city blocks and using two-way fixed effects differences-in-differences estimators, I show the gentrification of one block increases violence across the surrounding neighborhood. I find that some 2,400 (8%) of Philadelphia's shootings between the years 2011 and 2020 can be attributed to spillover effects from the gentrification of drug blocks. This effect …

Development Of A Framework To Support Community-Scale Nutrient Recovery For Local Crop Fertilization And Production, Scott A. Lopez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nutrient and resource recovery (NRR) has become increasingly crucial as regions face deteriorating sanitation infrastructures, limited support, and growing environmental challenges. Implementing region-specific NRR technologies and systems could provide effective circular economy insights into addressing these challenges. Because of the multidisciplinary challenges associated with the design and implementation of NRR strategies, various government and local decision-makers need to collaborate effectively in the decision-making process. Structured decision-making (SDM) methodologies are practical when determining the most appropriate NRR strategy for nutrient-rich waste streams. However, applications of computational SDM limited because of the vast amounts of data that are needed to analyze the …

Artificial Light At Night Disrupts Pain Behavior And Cerebrovascular Structure In Mice, Jacob Raymond Bumgarner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Artificial Light at Night Disrupts Pain Behavior and Cerebrovascular Structure in Mice

Jacob R. Bumgarner

Circadian rhythms are intrinsic biological processes that fluctuate in function with a period of approximately 24 hours. These rhythms are precisely synchronized to the 24- hour day of the Earth by external rhythmic signaling cues. Solar light-dark cycles are the most potent environmental signaling cue for terrestrial organisms to align internal rhythms with the external day. Proper alignment and synchrony of internal circadian rhythms with external environmental rhythms are essential for health and optimal biological function.

The modern human environment on Earth is no longer …

Advancing Medical Technology For Motor Impairment Rehabilitation: Tools, Protocols, And Devices, Matthew Yough

Graduate Theses, Dissertations, and Problem Reports (ETD)

Excellent motor control skills are necessary to live a high-quality life. Activities such as walking, getting dressed, and feeding yourself may seem mundane, but injuries to the neuromuscular system can render these tasks difficult or even impossible to accomplish without assistance. Statistics indicate that well over 100 million people are affected by diseases or injuries, such as stroke, Parkinson’s Disease, Multiple Sclerosis, Cerebral Palsy, peripheral nerve injury, spinal cord injury, and amputation, that negatively impact their motor abilities. This wide array of injuries presents a challenge to the medical field as optimal treatment paradigms are often difficult to implement due …

Outdoor Recreation Benefits In Urban Areas: An Importance-Performance Analysis Of Residents' Perception In Morgantown, West Virginia, Zachary D. Cox

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Outdoor Recreation Benefits in Urban Areas: An Importance-Performance Analysis of Residents’ Perception in Morgantown, West Virginia

Zachary Cox

Since the COVID-19 pandemic, participation in outdoor recreation has surged in primitive, rural, and even urban settings. Similarly, outdoor recreation studies that focus on residents have continually grown post-COVID-19. Through shared activities and a sense of community, residents have a strong voice in the decisions and improvements of their local environmental spaces. The purpose of this study is to assess the benefits of outdoor recreation among residents in the city of Morgantown, West Virginia. To identify these benefits and perceptions, researchers …

Localized Delivery Of Gm-Csf In The Regulation Of Breast Tumor Oxygen And Anti-Tumor Immunity Through Macrophages, Nicole E. Mihalik

Graduate Theses, Dissertations, and Problem Reports (ETD)

GM-CSF has been employed as an adjuvant to cancer immunotherapies in solid tumors due to its ability to drive immune stimulatory macrophages and other myeloid cells that promote anti-tumor responses. However, GM-CSF does not always exert immune stimulatory effects on myeloid cells, and sometimes even drives immune suppressive phenotypes. GM-CSF effects on myeloid cells and the tumor microenvironment (TME) appear to vary across dosages and even cancer types. However, an optimal dosing regimen for GM-CSF has yet to be determined to-date, and this is further complicated by the fact that GM-CSF is often administered via oncolytic viruses or tumor vaccines …

Elucidating Mechanisms Of Proteasome Modulation By Small Molecules To Treat Neurodegenerative Diseases And Cancer, Janelle Joo Yee Chuah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maintaining protein homeostasis, or proteostasis, is crucial for cellular function, and protein degradation through the proteasome plays a pivotal role in this process. Dysregulation of the proteasome can lead to proteostasis collapse and has been linked to the development of neurodegenerative diseases and cancers. Neurodegenerative diseases often exhibit proteasome impairment, whereas proteasome upregulation is frequently observed in cancer. Although the use of proteasome inhibitors as a pharmaceutical approach has shown success in certain cancers, it has yet to demonstrate efficacy in neurodegenerative diseases due to the absence of drug-like small molecules capable of effectively reversing proteasome impairment and/or activating the …

Spatial Processing Of Conspecific Signals In Weakly Electric Fish: From Sensory Image To Neural Population Coding, Oak Everette Milam

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this dissertation, I examine how an animal’s nervous system encodes spatially realistic conspecific signals in their environment and how the encoding mechanisms support behavioral sensitivity. I begin by modeling changes in the electrosensory signals exchanged by weakly electric fish in a social context. During this behavior, I estimate how the spatial structure of conspecific stimuli influences sensory responses at the electroreceptive periphery. I then quantify how space is represented in the hindbrain, specifically in the primary sensory area called the electrosensory lateral line lobe. I show that behavioral sensitivity is influenced by the heterogeneous properties of the pyramidal cell …

Expansion On The Use Of The Novel Compound, Elp-004, Using A Mycobacterium Tuberculosis Experimentation Model, Kendyl Marie Berry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Osteoarticular tuberculosis (OAT) is a bone degenerative disease that results in bone erosion, joint effusion, joint swelling, and sometimes, nerve compression. OAT occurs from the hematogenous dissemination of Mycobacterium tuberculosis (Mtb) bacilli spreading from the primary site of infection to the bones and joints; the primary site of infection tends to be the lungs as pulmonary tuberculosis infections are the most common. OAT can occur as the result of an active or latent infection. Latent infections result in OAT when bacilli are able to escape granulomas. The incidence of OAT is not as high as pulmonary infections, making up about …

Pedagogical Piano Works By Four Contemporary Composers: Emma Lou Diemer, Dianne Goolkasian Rahbee, Karen Tanaka And Chee-Hwa Tan, Manuel Alejandro Molina Flores

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research document aims to help diversify the pedagogical piano repertoire by featuring four contemporary composers living in the United States: Emma Lou Diemer, Dianne Goolkasian Rahbee, Karen Tanaka, and Chee-Hwa Tan. Their commitment to the creation of pedagogical works at the elementary, intermediate, and early-advanced levels gives students the opportunity to develop their piano technique while learning beautifully crafted pieces. The present document covers the life, formal education, important influences, and the compositional style of each composer. Additionally, it includes selected pieces at the late-elementary and early-intermediate levels where a discussion about their pedagogical benefits is included. Lastly, a …

Computational Mechanisms Of Face Perception, Jinge Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

The intertwined history of artificial intelligence and neuroscience has significantly impacted their development, with AI arising from and evolving alongside neuroscience. The remarkable performance of deep learning has inspired neuroscientists to investigate and utilize artificial neural networks as computational models to address biological issues. Studying the brain and its operational mechanisms can greatly enhance our understanding of neural networks, which has crucial implications for developing efficient AI algorithms. Many of the advanced perceptual and cognitive skills of biological systems are now possible to achieve through artificial intelligence systems, which is transforming our knowledge of brain function. Thus, the need for …

Influence Of Maternal Nano-Titanium Dioxide Inhalation Exposure On Cyclooxygenase Metabolites: Maternal Microvascular And Sexually Dimorphic Feto-Placental Outcomes., Julie Anne Griffith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nano-titanium dioxide (nano-TiO₂) is an engineered nanomaterial (ENM), which is widely utilized in diverse products like surface coatings, cosmetics, medical devices, and filters. Inhaled nano-TiO₂ has been demonstrated to induce pulmonary inflammation, impair microvascular function, and hinder generational reproductive endpoints. These outcomes indicate why it is important to explore gestation, as it requires timely vascular adaptations to support maternal and fetal health and development. Therefore, the central hypothesis of my dissertation is: maternal nano-TiO₂ inhalation exposure will impact maternal microvascular function and fetoplacental hemodynamics and function in a sexually dimorphic manner.

The first purpose of …

Motion Planning In Artificial And Natural Vector Fields, Bernardo Martinez Rocamora Junior

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation advances the field of autonomous vehicle motion planning in various challenging environments, ranging from flows and planetary atmospheres to cluttered real-world scenarios. By addressing the challenge of navigating environmental flows, this work introduces the Flow-Aware Fast Marching Tree algorithm (FlowFMT*). This algorithm optimizes motion planning for unmanned vehicles, such as UAVs and AUVs, navigating in tridimensional static flows. By considering reachability constraints caused by vehicle and flow dynamics, flow-aware neighborhood sets are found and used to reduce the number of calls to the cost function. The method computes feasible and optimal trajectories from start to goal in challenging …